Significant Differences In Blood Glucose Research Articles

Dextrose-containing intraoperative fluid in neonates: a randomized controlled trial.

Glucose requirement in neonates during surgery and the impact of glucose supplementation on neonatal metabolism remain unclear. This study was designed to identify an appropriate perioperative fluid regimen in neonates which maintains carbohydrate and lipid homeostasis. Forty-five neonates undergoing primary repair of a trachea-esophageal fistula were randomly allocated into three groups. During surgery, the neonates received either 1% dextrose in Ringer lactate (RL) (group D1) at 10 ml·kg(-1) ·h(-1) , or 2% dextrose in RL (group D2) at 10 ml·kg(-1) ·h(-1) , or 10% dextrose in N/5 saline at 4 ml·kg(-1) ·h(-1) and replacement fluid with 6 ml·kg(-1) ·h(-1) of RL (group D4). Glucose homeostasis, electrolyte balance, acid-base status, and endocrine and metabolic parameters were compared among the groups during the perioperative period. Blood glucose increased in all the three groups at the end of surgery, with no significant difference in blood glucose and incidence of hyperglycemia (BG > 150 mg·dl(-1) ) among them. At 24 h after surgery, blood glucose and incidence of hyperglycemia was significantly higher in Group D1 compared to Group D4. Base excess, bicarbonate, lactate, and pH showed a significant fall in Group D1. There was no significant difference in serum-free fatty acids, serum beta-hydroxy butyrate, and serum cortisol in three groups. At the end of surgery, serum insulin was significantly lower and glucagon : insulin (G : I) ratio was higher in Group D1 compared to Group D4. All three solutions, when infused at 10 ml·kg(-1) ·h(-1) , are equally effective in maintaining glucose homeostasis, but 1% dextrose-containing fluid promotes catabolism, insulin resistance, rebound hyperglycemia, and acidosis. Therefore, 2-4% dextrose-containing fluids is more suitable compared to 1% dextrose-containing fluids for use during major neonatal surgeries requiring average fluid infusion rate of 10 ml·kg(-1) ·h(-1) .

Comparative effect of intraduodenal and intrajejunal glucose infusion on the gut-incretin axis response in healthy males.

The region of enteral nutrient exposure may be an important determinant of postprandial incretin hormone secretion and blood glucose homoeostasis. We compared responses of plasma glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP), insulin and glucagon, and blood glucose to a standardised glucose infusion into the proximal jejunum and duodenum in healthy humans. Ten healthy males were evaluated during a standardised glucose infusion (2 kcal min−1 over 120 min) into the proximal jejunum (50 cm post pylorus) and were compared with another 10 healthy males matched for ethnicity, age and body mass index who received an identical glucose infusion into the duodenum (12 cm post pylorus). Blood was sampled frequently for measurements of blood glucose and plasma hormones. Plasma GLP-1, GIP and insulin responses, as well as the insulin:glucose ratio and the insulinogenic index 1 (IGI1) were greater (P<0.05 for each) after intrajejunal (i.j.) than intraduodenal glucose infusion, without a significant difference in blood glucose or plasma glucagon. Pooled analyses revealed direct relationships between IGI1 and the responses of GLP-1 and GIP (r=0.48 and 0.56, respectively, P<0.05 each), and between glucagon and GLP-1 (r=0.70, P<0.001). In conclusion, i.j. glucose elicits greater incretin hormone and insulin secretion than intraduodenal glucose in healthy humans, suggesting regional specificity of the gut–incretin axis.

Considerations for the design and interpretation of fishing release mortality estimates

To generate mortality estimates for fish that are captured and released in recreational and commercial fisheries, it is common to temporarily hold fish in captivity. Typically, captured fish are placed in some form of pen, cage or tank with control individuals, yet little is known about how the type of holding environment influences fish condition or mortality. Here we captured freshwater fish (bluegill; Lepomis macrochirus) via angling and fyke net and retained them in one of four holding environments; a round flow-through tank on shore [TANK], a knotless nylon pen with natural substrate in the lake [PEN], a knotless nylon floating cage with a rigid structure [RCAGE], and a knotless nylon floating cage that lacked rigid structure [CAGE]. Mortality was low (1%) across both capture techniques and holding environments during the 14-day retention period. All mortalities were associated with capture by fyke net. A chronic stress indicator, blood glucose, was determined for a subset of fish on day 5. Although there were significant differences in blood glucose between angled RCAGE and angled PEN (Tukey, P=0.047) and angled RCAGE and fyke PEN (Tukey, P=0.015), the observed levels were generally quite low (range: 1.0–3.9mmolL−1) and the differences were likely associated with differences in feeding; fish in the PEN group with access to substrate (and presumably the most food) had slightly higher glucose levels. At the conclusion of the study Fulton's condition factor was similar among all groups (ANOVA, P>0.05, all terms). However, fish held in the TANK treatment had the highest levels of external protozoan parasite infection by Ichthyopthirius (Tukey, P<0.05). This study illustrates that in situ holding environments (rather than tanks) may help reduce mortality, stress, and disease during studies that estimate post-release mortality. We encourage additional research to explore how the holding environment can influence inferences made about post-release mortality and sublethal impacts of fishing.

Ginsenoside Rb1 improves energy metabolism in the skeletal muscle of an animal model of postoperative fatigue syndrome

BackgroundPostoperative fatigue syndrome (POFS) is a common clinical complication followed by almost every major abdominal surgery. Ginsenoside Rb1 (GRb1), a principle ginsenoside in ginseng, could exert a potent anti-fatigue effect on POFS. However, the mechanism is still unknown. Previous studies revealed that alterations in the energy metabolism in the skeletal muscle may play a vital role in the development and progression of fatigue. In the present study, we investigate the effect of GRb1 on energy metabolism in the skeletal muscle of a rat model of POFS induced by major small intestinal resection. MethodsGRb1 (10 mg/kg) was intraperitoneally administrated once daily for 1, 3, 7, and 10 d from the operation day, respectively. The locomotor activity was recorded every day, and total food intake was calculated starting from 24 h after surgery. After GRb1 treatment was completed, blood and skeletal muscle were sampled. The level of blood glucose was determined by an automatic biochemical analyzer. The content of adenosine triphosphate (ATP) in skeletal muscle was determined by high-performance liquid chromatography. The activity of energy metabolic enzymes Na+-K+-ATPase, pyruvate kinase, and succinate dehydrogenase (SDH) was assessed by commercially available kits. ResultsThe results revealed that GRb1 could increase locomotor activity of POFS rats and significantly increase their total food intake postoperatively (P < 0.05). Furthermore, GRb1 also significantly increased ATP content in the skeletal muscle of POFS rats (P < 0.05). Meanwhile, the activity of Na+-K+-ATPase and SDH in the skeletal muscle of POFS rats was enhanced by GRb1 (P < 0.05). However, no significant differences in blood glucose and pyruvate kinase were found between the POFS and GRb1 treatment rats (P > 0.05). ConclusionsThese results suggest that GRb1 may improve skeletal muscle energy metabolism in POFS, and the underlying mechanism may be associated with an increase in the content of ATP and an enhancement in the activity of energy metabolic enzymes such as Na+-K+-ATPase ATPase and SDH in the skeletal muscle.

Abstract 9895: Metformin Attenuates Atherosclerosis and Vascular Aging Beyond Glycemic Control

Background: Cardiovascular disease due to atherosclerosis, the main cause of morbidity and mortality in most of the world, can be exacerbated by other age-related changes in blood vessels, including arterial stiffness mediated by angiotensin II (Ang II). Thus, strategies that can target atherosclerosis and vascular aging concomitantly are of special interest. Metformin, the first line medication for type 2 diabetes, was shown to attenuate all-cause mortality and myocardial infarction compared with other standard diabetes medications, suggesting that metformin has protective effects in the cardiovascular system beyond its glucose control activities. However, the exact mechanism(s) of action for metformin that are beyond its antihyperglycemic effects are still unknown. Methods and Results: We found that metformin even at concentrations as low as 20 μM can decrease Ang II-induced senescence of vascular smooth muscle cells. Using the ApoE-Knockout mice, we further tested whether metformin can diminish the progression of atherosclerosis and vascular aging and in response to Ang II treatment or high fat diet (HFD). We found that both Ang II induced hypertension and vascular aging were almost completely abolished by metformin treatment. Moreover, metformin treated animals had significantly less atherosclerotic plaque area compared with control groups. Similar vasculoprotective effects were observed when metformin was given to the mice fed HFD (Fig. 1). However, the vasculoprotective findings in both models were not accompanied by significant differences in blood glucose or cholesterol levels. Based on our ongoing studies, these effects of metformin could be explained, at least in part, by its role in modulation of the mTOR pathway that plays a major role in response to nutrition status. Conclusion: Metformin can be useful as a primary or secondary preventive therapy for patients at risk of suffering cardiovascular disease even in the absence of diabetes.

A Community-Based Pilot Study of a Diabetes Pharmacist Intervention in Latinos: Impact on Weight and Hemoglobin A1c

Type 2 diabetes disproportionately affects Latinos increasing their risk of diabetes-related complications. This study used a randomized controlled design with a community-based approach to evaluate the impact of a culturally tailored pharmacist intervention on clinical outcomes in Latino diabetics. The intervention included a focused discussion and two individual pharmacist counseling sessions on medication, nutrition, exercise, and self-care to promote behavior changes. Sessions were culturally adapted for language, diet, family participation, and cultural beliefs. Clinical outcomes were measured at baseline and three months. Nineteen intervention and 24 control participants completed the study. Mean BMI reduction was greater for intervention than for control group participants (-0.73 ± 0.07 kg/m2 versus + 0.37 ± 0.02 kg/m2 p<.009 respectively). Hemoglobin A1c was significantly reduced by 0.93 ± 0.45% in the intervention group only. There was no significant difference in blood glucose, blood pressure, or lipid levels. An innovative culturally-sensitive pharmacist intervention improved selected clinical outcomes among Latino diabetics.

Development of Polyvinyl Alcohol Bioartificial Pancreas with Rat Islets and Mesenchymal Stem Cells

To improve the function of the polyvinyl alcohol (PVA) bioartificial pancreas, we focused on bone marrow–derived mesenchymal stem cells (MSCs). We examined whether the function of PVA-encapsulated rat islets could be improved by coencapsulation with syngeneic MSCs. We macroencapsulated 1,500 rat islet equivalents (IEQ) with or without 1 × 106 MSCs with the use of 3% PVA solution before implantation intraperitoneally into diabetic BALB/c mice. We evaluated the function of the device in vitro (the residual rate, viability, and insulin-releasing function of the islets) and in vivo assessments (blood glucose and serum C-peptide changes after transplantation and glucose tolerance test). Although cultured islets also were destroyed, the shapes of the islets cocultured with MSCs were preserved but not different from encapsulated islets without MSCs. At 96 hours after culture the residual rates of islet recovery among those cocultured with versus without MSCs were 66% versus 39.5%, respectively, (P = .03). On the other hand, there was no significant difference between encapsulated islets with versus without MSCs. Furthermore, the stimulation index of the islets was improved by coculture with MSCs (2.6 ± 0.6 vs 1.4 ± 0.1; P = .03), but no beneficial effects were observed between islets encapsulated with versus without MSCs. The viability of islets cocultured with MSCs was significantly better than that without MSCs (84.2 ± 2.5 vs 73.3 ± 0.9; P = .037), but MSCs did not improve the viability of encapsulated islets. There were no significant differences in blood glucose or serum C-peptide between islets encapsulated with versus without MSCs. The histologic findings showed many degenerative islets and MSCs soon after transplantation. In conclusion, further studies are necessary to develop a novel PVA bioartificial pancreas that can be used with MSCs.

Using the T2DN rat as a model to determine therapeutic efficacy of Serelaxin (recombinant human relaxin‐2) for Diabetic Nephropathy

Recent studies demonstrate renoprotective and antifibrotic effects of relaxin in non‐diabetic kidney disease. To test the hypothesis that relaxin protects against diabetic renal injury, we infused Serelaxin (RLX, 4ug/h, Corthera, s.c. minipump) or vehicle into 10–12 month old male T2DN rats for 3 weeks (n=10/group). An ELISA confirmed delivery of RLX (21±2 ng/mL plasma, nondetectable in control). Baseline protein excretion (142±27 vs 144±39 mg/d) and blood glucose (322±32 vs 296±31 mg/dL) were similar in RLX and untreated T2DN, respectively. After treatment, RLX and control groups showed no significant differences in blood glucose (313±18 vs 288±23 mg/dL), MAP (130±8 vs 118±7 mmHg), or proteinuria (212±48 vs 199±63 mg/d). Masson's trichrome staining of kidney sections revealed significant glomerular and tubulointerstitial injury in both groups, with no remarkable changes following RLX treatment. There were no differences between the RLX and control groups (n=5) in renal blood flow (6.0±1.7 vs 6.9±2.6 mL/min) or glomerular filtration rate (3.2±1.3 vs 3.3±1.2 mL/min). We conclude that the T2DN rat is a suitable model for diabetic nephropathy as it does exhibit progressive proteinuria and renal injury similar to human diabetic nephropathy; however, 3 weeks of RLX treatment is not sufficient to reverse established renal injury present in the T2DN rat. Earlier and/or longer treatment with RLX may be required.

Effect of cinnamon on blood glucose, satiety and caloric intake in middle‐aged obese adults

The objective of this study was to examine the effect of cinnamon on postprandial blood glucose, satiety and subsequent caloric intake in middle‐aged obese adults. Using a crossover design, subjects consumed 50 g available carbohydrate as a breakfast drink with or without 6 g ground cassia cinnamon. Blood glucose and satiety were evaluated at 0, 15, 30, 45, 60, 90 and 120 minutes. Ad libitum caloric intake was estimated from a meal served at 240 minutes. Twenty‐six adults (9 males and 17 females), aged 40–63 years, completed the study. Paired t‐test, with Bonferroni correction, assessed differences in blood glucose, satiety and caloric intake between the control and cinnamon conditions. There were no significant differences in blood glucose and satiety values at any time point between the two conditions (p&gt;;0.05). Subsequent caloric intake was similar in the two conditions (p&gt;;0.05). The lack of agreement with earlier research may be related to differences in study populations and/or study foods.

Mineral and hormonal profile in acyclic and cyclic Murrah buffaloes during summer in Chhattisgarh

The experiment was conducted to study the incidence of cyclicity, biochemical profile (blood glucose, non esterified fatty acid (NEFA), macro minerals viz Ca, Pi, Mg, micro minerals viz Cu, Co, I, Zn, Mn, hormones, viz. progesterone and insulin in acyclic and cyclic Murrah buffaloes during summer (March to June 2009) in Chhattisgah. Out of 108 Murrah buffaloes 49 (45.37%) were found without palpable follicles and corpus luteum at per rectal examinations performed twice at 12 days apart, indicating true anestrus. The biochemical and hormonal profile were estimated in 6 cyclic and 24 acyclic Murrah buffaloes during summer. There was no significant difference in blood glucose and NEFA level in cyclic and acyclic buffaloes. The level of inorganic phosphorus and Mg were significantly lower in acyclic buffaloes than cyclic buffaloes. There was no significant difference in the level of calcium, however there was significant difference in Ca: P in cyclic and acyclic buffaloes. The micro minerals Zn, Fe, Cu, Co were significantly lower in acyclic buffaloes, however, there was no significant difference in the level of Mn and iodine. The serum progesterone level was significantly higher in cyclic than acyclic buffaloes. There was no significant difference in the level of insulin.

Effect of Ground Cinnamon on Postprandial Blood Glucose Concentration in Normal-Weight and Obese Adults

In healthy normal-weight adults, cinnamon reduces blood glucose concentration and enhances insulin sensitivity. Insulin resistance, resulting in increased fasting and postprandial blood glucose and insulin levels, is commonly observed in obese individuals. The objective of the study was to compare declines in postprandial glycemic response in normal-weight and obese subjects with ingestion of 6 g ground cinnamon. In a crossover study, subjects consumed 50 g available carbohydrate in instant farina cereal, served plain or with 6 g ground cinnamon. Blood glucose concentration, the main outcome measure, was assessed at minutes 0, 15, 30, 45, 60, 90, and 120. Repeated-measures analysis of variance evaluated the effects of body mass index (BMI) group, dietary condition, and time on blood glucose. Paired t-test assessed blood glucose at individual time points and glucose area under the curve (AUC) between dietary conditions. Thirty subjects between the ages of 18 and 30 years, 15 with BMIs between 18.5 and 24.9 and 15 with BMIs of 30.0 or more, completed the study. There was no significant difference in blood glucose between the two BMI groups at any time point. However, in a combined analysis of all subjects, the addition of cinnamon to the cereal significantly reduced 120-minute glucose AUC (P=0.008) and blood glucose at 15 (P=0.001), 30 (P<0.001), 45 (P<0.001), and 60 (P=0.001) minutes. At 120 minutes, blood glucose was significantly higher with cinnamon consumption (P<0.001). These results suggest cinnamon may be effective in moderating postprandial glucose response in normal weight and obese adults.

Effects of Astragalosides on Cultured Islets After Cryopreservation in Rats

Objective To explore the effects of AST (astragalosides) on cultured rat islet yield, purity, and function after cryopreservation in rats. Methods Pancreatic islets were isolated from 30 Sprague-Dawley rats using the standard technique of collagenase P digestion and discontinuous Ficoll gradient purification. After thaw, the islets were randomly divided into AST group and control group ( n = 15). Next, the islet cells were cultured in AST-containing medium or standard medium for 7, 14, and 21 days after cryopreservation and thaw. The quantity, purity, and survival rate were calculated in the two groups before and after culture. Then the in vitro and in vivo function was observed in diabetic rats after islet transplantation. Results The quantity and purity of islets had no difference between the two groups before culture ( P > .05) while the difference after culture was significantly ( P < .05). The survival rate of islets was 48% in AST group and 32% in the control group 21 days after thaw ( P < .05). After 3 days, there was significantly a higher simulation index in the AST group than in the control group ( P < .05). There was a significant difference in blood glucose and insulin concentrations between the groups after 3 days ( P < .05). Conclusion AST can be added to the culture medium to reduce the loss of islet cryopreservation and be intravenously injected to improve culture islet function in vitro and prolong islet graft survival in diabetic rats.

Developmental exposure to di(2-ethylhexyl) phthalate impairs endocrine pancreas and leads to long-term adverse effects on glucose homeostasis in the rat

-Di(2-ethylhexyl) phthalate (DEHP), a typical endocrine-disrupting chemical (EDC), is widely used as plasticizer. DEHP exposure in humans is virtually ubiquitous, and those undergoing certain medical procedures can be especially high. In this study, we investigated whether developmental DEHP exposure disrupted glucose homeostasis in the rat and whether this was associated with the early impairment in endocrine pancreas. Pregnant Wistar rats were administered DEHP (1.25 and 6.25 mg·kg(-1)·day(-1)) or corn oil throughout gestation and lactation by oral gavage. Body weight, glucose and insulin tolerance, and β-cell morphometry and function were examined in offspring during the growth. In this study, developmental DEHP exposure led to abnormal β-cell ultrastructure, reduced β-cell mass, and pancreatic insulin content as well as alterations in the expression of genes involved in pancreas development and β-cell function in offspring at weaning. At adulthood, female DEHP-exposed offspring exhibited elevated blood glucose, reduced serum insulin, impaired glucose tolerance, and insulin secretion. Male DEHP-exposed offspring had increased serum insulin, although there were no significant differences in blood glucose at fasting and during glucose tolerance test. In addition, both male and female DEHP-exposed offspring had significantly lower birth weight and maintained relatively lower body weight up to 27 wk of age. These results suggest that developmental exposure to DEHP gives rise to β-cell dysfunction and the whole body glucometabolic abnormalities in the rat. DEHP exposure in critical periods of development can be a potential risk factor, at least in part, for developing diabetes.

Effect of inborn pancreatic islet deficit in the Munich Wister Frömter rat

The total mass of pancreatic islet cells is a critical factor in glucose metabolic control. The aim of the present study was to investigate whether in the Munich Wistar Frömter (MWF) rat, beside a reduction in the number of nephrons, there are also alterations in the number of pancreatic islets and of β cell mass. We also examined glucose metabolism, both in normal conditions and following intravenous glucose injection. The number of islets per pancreas, estimated by morphometrical analysis, was significantly lower in MWF rats than in Wistar rats (3,501±1,285 vs. 7,259±2,330 islet/rat, respectively). Also the mean number of islets per gram of body weight was significantly lower in MWF rats than in Wistar rats (18±7 in MWF rats vs. 28±10 islets/g bw in Wistar rats). Morphometric analysis of β cell mass showed an average of 77.1±7% islet cells staining for insulin in MWF rats and 83.9±2.1% in the control Wistar rats. Despite the lower number of islets and β cells, MWF and Wistar rats had comparable fasting blood glucose levels but significant differences in blood glucose following an intraperitoneal glucose tolerance test. In summary, pancreatic islets of MWF and Wistar rats showed a marked difference in morphometrical characteristics. While this difference is not associated with blood glucose levels, glucose metabolism after IPGTT between MWF and Wistar rats is significantly different. These data suggest that an inborn deficit in β cell mass of about 60% is responsible for altered glucose metabolism and could favor the development of diabetes.

Prolongation of Islet Graft Survival Using Concomitant Transplantation of Islets and Vascular Endothelial Cells in Diabetic Rats

Background The transplantation of isolated islets of Langerhans is nearing acceptance as treatment of type 1 diabetes mellitus. Because the arterial and venous connections of the pancreas are disrupted during islet isolation, islets must be revascularized after transplantation. Objective To observe whether increased numbers of vascular endothelial cells in islets can affect the angiogenesis and function of the grafts. Materials and Methods Rats with streptozocin-induced diabetes were divided into 3 groups. The rats in group 1 received islet grafts under the capsule of the left kidney; rats in group 2 received combined vascular endothelial cell and islet transplants; and rats in group 3 served as controls. After the transplantation procedure, blood glucose and insulin concentrations were evaluated daily. Hematoxylin-eosin and immunohistochemical staining was used to detect expression of vascular endothelial growth factor antibodies in the diabetic rat kidneys. The mean microvascular density was also calculated. Results At 3 days posttransplantation, blood glucose and insulin concentrations returned to normal in group 2, however, they declined only slightly in group 1, and moderate hyperglycemia was present. There was a significant difference in blood glucose and insulin concentrations between the 2 groups after 3 days ( P < .05). The mean (SD) microvascular density in group 2 was markedly higher than that in group 1 (12.58 [1.81] vs 10.38 [0.97] P = .04). Conclusion This study suggests that concomitant transplantation of isolated islets with endothelial cells can prolong islet graft survival in diabetic rats.

Anti-diabetic effect of camel milk in alloxan-induced diabetic dogs: a dose-response experiment

This study was conducted to evaluate the effect of camel milk in alloxan-induced diabetic dogs and to follow this effect at three doses of milk. Firstly, three groups of dogs were used: two groups composed each of four diabetic dogs and receiving raw camel milk (treatment 1) or cow milk (treatment 2), and four healthy dogs getting raw camel milk (treatment 3) were used as control. Each animal was treated with 500 ml of milk daily. Secondly, we compared the effects of three amounts of camel milk: 100 ml, 250 ml and 500 ml to treat the diabetic dogs. After week 3, the dogs treated with camel milk showed a statistically significant decrease in blood glucose (from 10.88 +/- 0.55 to 6.22 +/- 0.5 mmol/l) and total protein concentrations (from 78.16 +/- 2.61 g/l to 63.63 +/- 4.43 g/l). For cholesterol levels, there was a decrease from week 2 (from 6.17 +/- 0.5 mmol/l to 4.79 +/- 0.5 mmol/l). There were no significant difference in blood glucose, cholesterol or total protein concentrations in dogs drinking 250 and 500 ml of camel milk. The dogs treated with 100 ml of camel milk did not show any significant decrease in blood glucose levels, and cholesterol and total protein concentrations. The investigation was not limited to the improvement in glycemic balance, lipids and proteins control in diabetic dogs getting camel milk, but we also noted a stability of this state after the dogs stopped to drink milk. This effect depended on the quantity of camel milk used to treat diabetic dogs.

The effect of seamustard on blood lipid profiles and glucose level of rats fed diet with different energy composition

Recently, Korean people are consuming seaweeds almost 3.5 times more now than three decades ago. It is well known that seaweeds contain lots of soluble dietary fiber in addition to micronutrients such as β-carotene, iodine and some bioactive components. Seaweeds are considered to be effective for preventing chronic diseases including obesity, diabetes mellitus, atherosclerosis, cancer or constipation. This study was conducted to investigate the effect of seamustard intake on body weight gain, blood glucose level and lipid profiles in rats fed diets with different energy nutrient composition. Male Sprague-Dawley rats (average initial weight 103.7 g) were divided into groups for two experiments as follows; Control, M2.5 & M5 groups (Exp. I) and M5, M10, HCM5, HCM10, HFM5 & HFM10 groups (Exp. II). The rats were fed diet and water ad libitum for 4 weeks. In general, there was no significant difference in blood glucose and triglyceride concentration among groups. In Exp. I, serum LDL-cholesterol level of rats fed diet with 5% seamustard powder (M5) was significantly lower than that of control group, while HDL-cholesterol level, TC/LDL ratio and weight of adrenal gland were higher. In Exp. II, food intake, body weight gain and EER of high fat diet with 10% seamustard group (HFM10) were the lowest among groups. Except gastrocnemius muscle, all organ weights of HFM10 group were the lowest. Fecal cholesterol excretion and serum LDL-cholesterol concentration of HFM10 group were the highest, while serum HDL-cholesterol level was the lowest among groups. Interestingly, HDL-cholesterol concentration was the highest in HCM5 group among groups. From these results, it was suggested that seamustard intake might be more effective for body weight control, but not for improving blood lipid profiles in high fat diet than in high carbohydrate diet.

Equivalence of Earlobe Site Blood Glucose Testing With Finger Stick

This study determines if there are significant differences in blood glucose sampled at the earlobe relative to fingertip sites. A 50-participant cross-sectional comparative design was conducted at a 480-bed community hospital. Four trained data collectors obtained demographic information and blood glucose samples from both the fingertip and earlobe sites. Data analysis included descriptive statistics, paired t test, and the Bland Altman technique, assessing agreement between two methods of clinical measurements. Results indicate that the mean finger stick glucose result was statistically significantly greater than the mean earlobe glucose result. The Bland Altman plot did not demonstrate clinical significance. Results suggest that the earlobe may be a viable alternative to exclusive fingertip site when frequent sampling is necessary in acutely ill patients and demonstrate the importance of the role of the clinical bedside nurse in implementing evidence-based nursing practice related to diabetes treatment and management.

화학적 변성 및 생감자 전분이 고지방식이로부터 유도된 비만 흰쥐의 지질대사 및 항산화계에 미치는 영향

AIN-93G 식이조성을 기본으로 corn starch를 potato starch로 100% 대치한 고지방식이를 42일간 rat에 급여하여 비만을 유도 후, GPS군, SPS군, EZ군, H40군으로 나누어 70일 급여한 후 지질대사에 미치는 영향을 살펴보았다. 그 결과, 체중증가량은 GPS, EZ, H40군에서 차이가 없었고 식이효율 또한 유의적으로 차이가 없었다. 혈중 혈당농도, TC, LDL-cholesterol 농도는 변성 전분 섭취 군(EZ, H40군)에서 높았고, atherogenic index는 H40이 다른 군에 비교해 높게 측정되어 H40군이 비만, 동맥경화에 미치는 영향이 크게 나타났다. 간과 신장 조직의 MDA 생성량은 H40군이 혈청 MDA 생성량이 다른 군에 비해 매우 높았고, 간과 신장조직에서는 EZ, H40군이 낮았다. Glutathione 함량은 각 조직별로 경향이 달랐으나, GP-x활성은 SPS군에서 낮았다. 본 연구에서 전분을 달리하여 첨가한 고지방 식이가 비만한 식이에 미치는 영향을 살펴본 결과, 알감자 전분을 100% 대치하여 제조한 SPS군 및 효소 처리한 변성전분(EZ)을 100% 대치하여 급여한 군에서 비만억제 효과가 나타났다. 따라서 변성전분의 심혈관계 질환 및 혈중 지질 패턴 변화에 관련된 기전 연구가 지속적으로 필요하며, 생리활성이 규명된 후 다양한 가공식품 개발이 이루어져야 할 것으로 사료된다.

The effect of salatrim, a low-calorie modified triacylglycerol, on appetite and energy intake

BackgroundSalatrim is modified triacylglycerol that is rich in short-chain fatty acids and stearic acid. It is used as a lower-calorie fat replacer. In addition, it has been hypothesized that salatrim's reduced absorption in the small intestine may lead to greater amounts of fat in the gastrointestinal tract, which may decrease appetite and energy intake through the release of appetite-regulating gastrointestinal hormones. ObjectiveWe aimed to compare the effects of salatrim and traditional fat on appetite, ad libitum energy intake, and gastrointestinal hormones. DesignTwenty-two healthy, young, normal-weight men participated in a randomized, double-blind, crossover study. Test meals were a traditional fat meal and a salatrim meal with a mixture of traditional fat and salatrim. Visual analogue scales were used to record appetite and well-being every 30 min, and blood was sampled frequently. An ad libitum lunch was served 4.5 h after the test meal. ResultsThe salatrim meal increased fullness (P = 0.04) and decreased hunger (P = 0.06) significantly more than did the traditional fat meal. The traditional fat meal increased well-being (P = 0.02). There was no significant difference in ad libitum energy intake or overall energy intake between the 2 test days. No significant differences in blood glucose, insulin, triacylglycerol, ghrelin, cholecystokinin, glucagon-like peptide-1, or peptide YY concentrations were found. A significantly (P = 0.01) smaller increase in free fatty acids was observed after the salatrim meal than after the traditional fat meal. ConclusionsSalatrim had a modestly more suppressive effect on appetite than did a traditional fat. Gastrointestinal hormones did not seem to be involved.