The alcohol consumption of C57BL/Crgl mice decreased significantly when animals of this strain were injected with a solution of 0.2 mg of hydrocortisone acetate (a glucocorticoid) every second day. No such significant decrease in alcohol consumption occurred when C3H/Crgl/2 mice were injected with the same solution daily. When administered a solution of 0.5 mg of deoxycorticosterone acetate (a mineralocorticoid), neither the C57BL/Crgl injected daily or every other day nor the C3H/Crgl/2 injected daily displayed a significant decrease in alcohol consumption. The results indicate possible involvement of glucocorticoids, rather than mineralocorticoids, in the alcohol preference of mice.