Significance Of Superoxide Dismutase Research Articles

ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ КОМПОНЕНТОВ СИСТЕМЫ АНТИОКСИДАНТНОЙ ЗАЩИТЫ КАК МАРКЕРОВ ОРГАНИЧЕСКОГО ПОРАЖЕНИЯ ЦНС В НЕОНАТАЛЬНОМ ПЕРИОДЕ У НЕДОНОШЕННЫХ НОВОРОЖДЕННЫХ ДЕТЕЙ

Objective of the study: to assess the significance of superoxide dismutase (SOD) and glutathione as markers of severe CNS damage in premature infants. Materials and methods of research: a onestage comparative study with a control group – a part of an observational prospective longitudinal non-randomized multicenter study. The main group – 60 premature infants with gestational age (GA) less than 36 weeks inclusive (32 boys (53%) and 28 girls (47%), the control group – 25 fullterm newborns (15 boys (60%) and 10 girls (40%). In the process of dynamic observation, the main group was divided into subgroups depending on the diagnosed in the neonatal period according to instrumental methods of examination of CNS lesions. Subgroup 1 included 25 children with CNS lesions – intraventricular hemorrhages (IVH) of varying severity, periventricular leukomalacia – PVL; 14 boys (56%), 11 girls (44%). Subgroup 2 included 35 preterm infants without CNS damage – 18 boys (51%), 17 girls (49%) The indicators of oxidative stress, antioxidant capacity, blood levels of SOD and glutathione enzymes in reduced (GSH) and oxidized (GSSG) states were studied once in the early neonatal period. Results: statistically significant differences were found when comparing the control group, 1st and 2nd subgroups by body weight (BW) (K–V, p<0.0001). BW at birth in premature infants with CNS lesions was statistically significantly less than in premature infants without CNS lesions (M–U, p=0.0019). When analyzing GA, statistically significant differences were also found when comparing the three groups with each other (K–U, p<0.0001). The gestational period of premature infants with CNS lesions was statistically significantly lower than in premature infants without CNS lesions (M–U, p=0.0013). All three groups differed statistically significantly in terms of SOD (C–V, p=0.0045). Premature infants with CNS lesions in the neonatal period had a statistically significantly lower SOD value than children without CNS lesions (M–U, p=0.0021). Statistically significant differences were found when comparing the three groups in terms of GSSG content (K–V, p<0.0001). Also, in premature infants with CNS lesions, the GSSG values were statistically significantly lower than in premature infants without CNS lesions in the neonatal period (M–V, p=0.0001). The GSH level in all studied groups was statistically significantly different (C–C, p<0.0001). In premature infants with CNS lesions, the GSH level was statistically significantly lower than in infants without CNS lesions in the neonatal period (М–У, p=0.0001). When determining the TAS/TAC (antioxidant capacity), statistically significant differences were found when comparing the three groups (K–V, p=0.0002). In premature infants with CNS damage, TAS/TAC was statistically significantly lower than in the group of premature infants without CNS damage in the neonatal period (M–U, p=0.0001). In all cases of comparison of the studied parameters, the control group and premature babies without CNS damage did not differ statistically significantly. When comparing premature infants with and without damage to the CNS with GA of more than 31 weeks, it was revealed that the groups differed statistically significantly in all the studied parameters: in the group of children with CNS lesions, the studied parameters were lower (SOD, M–U, p=0.0249; GSSG, M–U, p=0.0055; GSH M–U, p=0.0055; TAS/TAC, p=0.0019). The study revealed that premature infants with CNS damage experience greater oxidative stress than infants without CNS damage in the neonatal period (Fisher's exact test, two-sided, p=0.0012, Infinity [NaN; Infinity]). Conclusions: Premature infants differ in antioxidant protection; children with lower antioxidant status are prone to the development of severe organic lesions of the central nervous system in the neonatal period. SOD and glutathione can be used in clinical practice as markers of organic lesions of the central nervous system in the neonatal period in premature infants.

Manganese Superoxide Dismutase Expression Correlates with a Poor Prognosis in Gastric Cancer

Objective: The biologic significance of superoxide dismutase (SOD) in transformed gastric carcinoma cells is unclear. The aim of this study was to examine the role of SOD as a prognostic indicator of gastric carcinomas and its association with other clinicopathological factors. Methods: Expression of MnSOD and Cu/ZnSOD was evaluated immunohistochemically in gastric carcinomas and adenomas using tissue-array methods. The correlation between SOD immunoreactivity and clinical outcome or clinicopathological factors was investigated. Results: MnSOD expression was associated with a poor patient prognosis and was strong in advanced gastric cancer, in the well-differentiated type and in the presence of lymphoid stroma (p < 0.05). On the other hand, there was no association between Cu/ZnSOD expression and patient survival. Cu/ZnSOD immunoreactivity was strong in advanced gastric cancer and in the presence of lymphoid stroma (p < 0.05) and was weak in signet ring cell type. Conclusion: MnSOD immunoreactivity is significantly associated with poor outcome in gastric carcinoma patients although both MnSOD and Cu/ZnSOD have a connection with several clinicopathological parameters with some overlap.

Significance of superoxide dismutase (SOD) in human colorectal cancer tissue: correlation with malignant intensity.

The significance of superoxide dismutase (SOD) activity in colorectal cancer tissue was determined from the aspect of the antioxidant defense system. SOD activity and thiobarbituric acid reactive substance were measured in the tumor, in tissues adjacent to the tumor, and in regions that appeared normal, and the results were analyzed in terms of various histopathological factors (stage of disease, depth of invasion, venous invasion, etc.). DNA ploidy pattern and cell proliferation in cancer tissue were also measured, and the results analyzed in relation to SOD activity. SOD activity in cancer tissue was higher than in the other two regions. SOD activity in cancer tissue increased with the progression of stage, and changed with the depth of invasion. There was a significant difference in SOD activity between patients with venous invasion and those in whom this was absent. Stepwise regression analysis suggested that venous invasion was the most significant factor influencing SOD activity. The proliferation index was high in cancer tissue with low SOD activity. The incidence of aneuploidy was high in cancer with high SOD activity, whereas the incidence of diploidy was high in cancer with low SOD activity. These results suggest that elucidation of the antioxidant system in cancer tissue can provide us with a better strategy for cancer treatment.