Fatty Acid Signatures Research Articles

Prolonged heavy rainfall and land use drive catchment sediment source dynamics: Appraisal using multiple biotracers

Excessive sediment loss degrades freshwater quality and is prone to further elevation and variable source contributions due to the combined effect of extreme rainfall and differing land uses. To quantify erosion and sediment source responses across scales, this study integrated work at both field and catchment scale for two hydrologically contrasting winters (2018–19 and 2019–20). Sediment load was estimated at the field scale (grassland-arable conversion system). Sediment source apportionment work was undertaken at the catchment scale (4.5 km2) and used alkanes, and both free and bound fatty acid carbon isotope signatures as diagnostic fingerprints to distinguish sediment sources: arable, pasture, woodland and stream banks. Sediment source apportionment based on bound fatty acids revealed a substantial shift in contributions, from stream banks dominating (70 ± 5%) in winter 2018–19, to arable land dominating (52 ± 7%) in the extreme wet winter 2019–20. Increases in sediment contributions from arable (∼3.9 times) and pasture (∼2.4 times) land at the catchment outlet during the winter 2019–20 were consistent with elevated sediment losses monitored at the field scale which indicated that low-magnitude high frequency rainfall alone increased sediment loss even from pasture by 350%. In contrast, carbon isotope signatures of alkanes and free fatty acids consistently estimated stream banks as a dominant source (i.e., ∼36% and ∼70% respectively) for both winters regardless of prolonged rainfall in winter 2019–20. Beyond quantifying the shifts in field scale sediment load and catchment scale sediment sources due to the changes in rainfall patterns, our results demonstrate valuable insight into how the fate of biotracers in soil and sediment manifests in the δ13C values of homologues and, in turn, their role in information gain for estimating sediment source contributions. Discrepancies in the estimated sediment source contributions using different biotracers indicate that without a careful appreciation of their biogeochemical limitations, erroneous interpretation of sediment source contributions can undermine management strategies for delivering more sustainable and resilient agriculture.

A Tailored Lipid Supplement Restored Membrane Fatty Acid Composition and Ameliorates In Vitro Biological Features of Human Amniotic Epithelial Cells.

Cell culture conditions influence several biological and biochemical features of stem cells (SCs), including the membrane lipid profile, thus limiting the use of SCs for cell therapy approaches. The present study aims to investigate whether the in vitro culture may alter the membrane fatty acid signature of human Amniotic Epithelial Cells (hAECs). The analysis of the membrane fatty acid composition of hAECs cultured in basal medium showed a loss in polyunsaturated fatty acids (PUFA), in particular in omega-6 (ω-6) content, compared to freshly isolated hAECs. The addition to the basal culture medium of a chemically defined and animal-free tailored lipid supplement, namely Refeed®, partially restored the membrane fatty acid signature of hAECs. Although the amelioration of the membrane composition did not prolong hAECs culture lifespan, Refeed® influenced cell morphology, counteracted the onset of senescence, and increased the migratory capacity as well as the ability of hAECs to inhibit Peripheral Blood Mononuclear Cell (PBMC) proliferation. This study provides new information on hAEC features during culture passages and demonstrates that the maintenance of the membrane fatty acid signature preserved higher cell quality during in vitro expansion, suggesting the use of lipid supplementation for SC expansion in cell-based therapies.

Occurrence and Distribution of Persistent Organic Pollutants (POPs) in Amphibian Species: Implications from Biomagnification Factors Based on Quantitative Fatty Acid Signature Analysis.

Contaminants pose a great threat to amphibian populations, but the bioaccumulation and distribution of contaminants in amphibians are still unclear. Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) had median concentrations of 468-3560 ng/g lipid weight (lw) and 206-2720 ng/g lw in the muscle of amphibians, respectively. BDE 209 was the predominant PBDE congener, while CBs 118, 138, 153, and 180 were the main PCB congeners. The diet compositions of amphibians were estimated by quantitative fatty acid signature analysis (QFASA). Dragonfly contributed the most to the diet of amphibians. Biomagnification factors (BMFs) based on quantitative amphibian/insect relationships showed more credible results than BMFs based on amphibian/each insect or amphibian/combined prey relationships. BMFs derived from QFASA declined with log KOW from 5 to 6.5 and then showed a parabolic relationship with log KOW greater than 6.5. BMFs of PCBs were significantly influenced by the elimination capacity of PCBs in amphibians. Less-hydrophobic PCBs preferentially accumulated in the skin than in muscle, which was probably due to the dermal exposure of less-hydrophobic PCBs for amphibians. The biomagnification and distribution of contaminants may be affected by multiple exposure pathways and the toxicokinetics of contaminants in various life stages of amphibians.

A critical review of the use of lipid signature molecules for the quantification of arbuscular mycorrhiza fungi

Tools for accurate measurements of microbial biomass/abundance are imperative in soil ecology. This perspective was prompted by a lack of consistency in how the neutral lipid fatty acid (NLFA) and phospholipid fatty acid (PLFA) 16:1ω5 are used to estimate arbuscular mycorrhizal (AM) fungal abundance, which we argue can lead to erroneous conclusions within studies and complicate among-study comparisons. We show that more than half of all studies published to date use PLFA 16:1ω5 to quantify AM fungal biomass without proper controls. This is problematic because AM fungi cannot be separated from the bacterial contribution to this fatty acid signature. We provide a set of recommendations for future research and specifically urge researchers to extract and analyze NLFA 16:1ω5 for more accurate and sensitive assessments of AM fungal biomass as this can be done without doubling effort.

Marine and terrestrial nitrifying bacteria are sources of diverse bacteriohopanepolyols

Hopanoid lipids, bacteriohopanols and bacteriohopanepolyols, are membrane components exclusive to bacteria. Together with their diagenetic derivatives, they are commonly used as biomarkers for specific bacterial groups or biogeochemical processes in the geologic record. However, the sources of hopanoids to marine and freshwater environments remain inadequately constrained. Recent marker gene studies suggest a widespread potential for hopanoid biosynthesis in marine bacterioplankton, including nitrifying (i.e., ammonia- and nitrite-oxidizing) bacteria. To explore their hopanoid biosynthetic capacities, we studied the distribution of hopanoid biosynthetic genes in the genomes of cultivated and uncultivated ammonia-oxidizing (AOB), nitrite-oxidizing (NOB), and complete ammonia-oxidizing (comammox) bacteria, finding that biosynthesis of diverse hopanoids is common among seven of the nine presently cultivated clades of nitrifying bacteria. Hopanoid biosynthesis genes are also conserved among the diverse lineages of bacterial nitrifiers detected in environmental metagenomes. We selected seven representative NOB isolated from marine, freshwater, and engineered environments for phenotypic characterization. All tested NOB produced diverse types of hopanoids, with some NOB producing primarily diploptene and others producing primarily bacteriohopanepolyols. Relative and absolute abundances of hopanoids were distinct among the cultures and dependent on growth conditions, such as oxygen and nitrite limitation. Several novel nitrogen-containing bacteriohopanepolyols were tentatively identified, of which the so called BHP-743.6 was present in all NOB. Distinct carbon isotopic signatures of biomass, hopanoids, and fatty acids in four tested NOB suggest operation of the reverse tricarboxylic acid cycle in Nitrospira spp. and Nitrospina gracilis and of the Calvin-Benson-Bassham cycle for carbon fixation in Nitrobacter vulgaris and Nitrococcus mobilis. We suggest that the contribution of hopanoids by NOB to environmental samples could be estimated by their carbon isotopic compositions. The ubiquity of nitrifying bacteria in the ocean today and the antiquity of this metabolic process suggest the potential for significant contributions to the geologic record of hopanoids.

Distinct gut microbiomes in two polar bear subpopulations inhabiting different sea ice ecoregions

Gut microbiomes were analyzed by 16S rRNA gene metabarcoding for polar bears (Ursus maritimus) from the southern Beaufort Sea (SB), where sea ice loss has led to increased use of land-based food resources by bears, and from East Greenland (EG), where persistent sea ice has allowed hunting of ice-associated prey nearly year-round. SB polar bears showed a higher number of total (940 vs. 742) and unique (387 vs. 189) amplicon sequence variants and higher inter-individual variation compared to EG polar bears. Gut microbiome composition differed significantly between the two subpopulations and among sex/age classes, likely driven by diet variation and ontogenetic shifts in the gut microbiome. Dietary tracer analysis using fatty acid signatures for SB polar bears showed that diet explained more intrapopulation variation in gut microbiome composition and diversity than other tested variables, i.e., sex/age class, body condition, and capture year. Substantial differences in the SB gut microbiome relative to EG polar bears, and associations between SB gut microbiome and diet, suggest that the shifting foraging habits of SB polar bears tied to sea ice loss may be altering their gut microbiome, with potential consequences for nutrition and physiology.

Association between fatty acid signature and growth rate of larval Pacific bluefin tuna in two major spawning grounds

Fatty acid composition and total fatty acids of field-caught Pacific bluefin tuna (PBF) Thunnus orientalis larvae were investigated to identify relationships between spawning ground, physiological condition, and growth rate. Multivariate statistical analyses revealed that both growth rate and environmental conditions at spawning grounds were substantially associated with variations in fatty acid compositions. Fast-growing larvae typically contained more α-linolenic acid and 22:5n-3, which are important as metabolic precursors of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Larval PBF fatty acid compositions differed by spawning ground; larvae caught around the Nansei Islands contained more 15:0, 17:0, 19:0, and 22:5n-6, and less EPA and arachidonic acid (ARA) than those from the Sea of Japan. Differences in larval odd-numbered fatty acid compositions might indicate different degrees of dependence on microbial loop energy supply between spawning grounds. Environments subject to sudden changes, such as those in water temperature and prey density in the Sea of Japan, might cause variability in fatty acid profiles, including extremely low %DHA. We suggest that continuous food intake and subsequent fatty acid catabolism for energy generation would be needed to facilitate fast growth.

Fecal DNA metabarcoding shows credible short-term prey detections and explains variation in the gut microbiome of two polar bear subpopulations

This study developed and evaluated DNA metabarcoding to identify the presence of pinniped and cetacean prey DNA in fecal samples of East Greenland (EG) and Southern Beaufort Sea (SB) polar bearsUrsus maritimussampled in the spring of 2015-2019. Prey DNA was detected in half (49/92) of all samples, and when detected, ringed sealPusa hispidawas the predominant prey species, identified in 100% (22/22) of EG and 81% (22/27) of SB polar bear samples with prey DNA detected. Bearded sealErignathus barbatusDNA was found in 19% (5/27) of SB polar bear samples for which prey DNA was detected. Prey DNA detection frequencies and relative abundances were compared to estimates from quantitative fatty acid signature analysis (QFASA) for a subset of SB polar bears. Ringed seal and bearded seal were the main prey identified by both methods, but QFASA also identified 2 cetacean prey species not found by prey DNA. Differences in DNA metabarcoding vs. QFASA results were likely related to the different dietary timescales captured by each approach, i.e. short-term vs. long-term diet, respectively. Prey DNA detection, sex/age class, and subpopulation significantly explained variation in polar bear gut bacterial composition. Polar bear samples with prey DNA detected were associated with higher abundances of the bacterial classes Clostridia and Bacilli and lower abundances of Negativicutes. Fecal DNA metabarcoding is thus useful for identifying recent prey of polar bears, complementing quantitative and likely longer-term QFASA estimates, and may help understand variation in the polar bear gut microbiome.

First evaluation of quantitative fatty acid signature analysis (QFASA) in dolphins

Accurate diet estimation of marine mammals is fundamental to investigate their ecological roles in marine habitats, but it remains a pervasive challenge to researchers due to the constraints associated with conventional methods based on stomach contents or feces analysis, which are imprecise, limited to last meal and often biased. Quantitative fatty acid signature analysis (QFASA), an emerging method for diet estimations, has been demonstrated to be reliable in reconstructing long-term diets of many marine mammal predators; however, its application has not been seriously evaluated in dolphins. To this end, we compared the QFASA estimates obtained using 8 pre-existing calibration coefficient sets, 3 fatty acid subsets, and 3 prey library subsets to the true diet of a captive Indo-Pacific humpback dolphin (Sousa chinensis) that was fed a long-term diet of mostly gizzard shad (Clupanodon thrissa) with smaller proportions of Japanese seabass (Lateolabrax japonicus), pumpkinseed (Lepomis gibbosus), roughscale tonguesole (Cynoglossus lida), and Chinese herring (Ilisha elongate). The diet of the dolphin was best reconstructed using the calibration coefficients derived from captive feeding trials of American mink (Mustela vison) that were fed herring with the “main” fatty acid subset; this process correctly identified gizzard shad as the main prey source of the dolphin with the highest actual diet proportions and lowest predator signature proportions outside the range of the mean prey proportions. In addition, high accuracy and consistency were acquired in the diet estimations obtained using the calibration coefficients derived from minks fed herring and herring plus seal oil even though pseudo-prey were included in our model, revealing the high robustness and reliability of QFASA in reconstructing the dolphin diet. We recommend caution in selecting fatty acid subsets when using fatty acid signatures derived from different studies. Overall, QFASA offers a promising tool to quantify the long-term diets of humpback dolphins and other odontocetes.

Quantitative fatty acid signature analysis (QFASA) in indoor dust: Implication for tracking indoor source accumulation of organic pollutant exposure.

Indoor dust has been used as a proxy for estimating human indoor pollutant exposure risks, yet source identification remains challenging. This study tentatively investigated whether quantitative fatty acid signature analysis (QFASA) of dust, could be applied to indicate sources and their respective contributions for a major class of indoor organic pollutants organophosphate flame retardants (OPFRs). We observed significant correlations between OPFR concentrations and lipid content (p<0.05) in house dusts. Using 15 signature fatty acids (FAs) in various indoor sources and the QFASA model, we found that clothing (39.1% in Australia and 36.5% in China) was the predominant contributing vector of dust OPFR followed by cooking oil and pet hair. Among these sources, clothing materials were proposed to be important vectors introducing organic pollutants to the indoor environment. Our QFASA contribution estimation analyses allowed for accurate prediction of most OPFR concentrations in clothing, validating our findings that clothing materials may serve as important carrier for OPFRs in indoor migration. This is the first study attempting to identify sources of organic pollutants using QFASA in an indoor setting and will provide important insight into the transfer of organic pollutants in indoor environment.

Assessing the migratory histories, trophic positions, and conditions of lake sturgeon in the St. Croix and Mississippi Rivers using fin ray microchemistry, stable isotopes, and fatty acid profiles

BackgroundReproducing populations of invasive carps (Hypophthalmichthys spp.) could alter aquatic food webs and negatively affect native fishes in the Mississippi National River and Recreation Area (MISS) and the St. Croix National Scenic Riverway (SACN). However, proposed invasive carp barriers may also threaten populations of native migratory fishes by preventing movements of fish between rivers that are necessary for life history requirements. In this study, nonlethal chemical techniques were used to provide baseline data related to the condition, trophic position, and migratory histories of lake sturgeon (Acipenser fulvescens) captured in the Mississippi and St. Croix Rivers.ResultsFish length and weight measurements and age estimates determined from pectoral fin rays demonstrated that lake sturgeon from the Mississippi River had greater lengths-at-age compared to sturgeon from the St. Croix River. However, length–weight relations were similar for sturgeon from the Mississippi and St. Croix Rivers. Lake sturgeon captured from different locations had distinguishable fatty acid signatures, and stable isotope analyses demonstrated that lake sturgeon from the Mississippi River generally feed at a higher trophic level than those in the St. Croix River. Strontium-to-calcium ratios (Sr:Ca) from fin ray cross sections indicated that sturgeon captured from the Mississippi River had higher Sr:Ca values than sturgeon captured from the St. Croix River, and natal origins and capture locations were not significantly different among sturgeon captured within individual rivers. Most sturgeon were captured in water with a similar Sr:Ca signature as their natal waters, indicating that there is some separation between populations of lake sturgeon in the St. Croix and Mississippi Rivers. However, Sr:Ca data indicated substantial variation in movement patterns among individual lake sturgeon, indicating that populations interact through migrations of individual fish between rivers.ConclusionsStudy results provide baseline condition and food web structure index data for assessing changes in lake sturgeon populations should invasive carps become established in these areas of the Mississippi and St. Croix Rivers. Controlled-exposure and telemetry studies would help verify and enhance the relations between Sr:Ca signatures in water and lake sturgeon pectoral fin rays to further assess mixing of sturgeons between rivers.

Evaluating the Lipid Quality of Yellowfin Tuna (Thunnus albacares) Harvested from Different Oceans by Their Fatty Acid Signatures.

The aim of this study was to evaluate the influence of the fishing location on yellowfin tuna’s (YFT; Thunnus albacares) white muscle total lipid (TL) content and fatty acid profile. The comparison included 45 YFT loins, equally divided between the Atlantic, Indian and Pacific Oceans. The ocean had no significant influence on YFT TL content, total PUFA and total n-3 PUFA (p > 0.05), averaging 1.09 g/100 g of muscle, 229.2 and 192.8 mg/100 g of muscle, respectively. On the other hand, the YFT harvested on the Indian Ocean displayed significantly higher contents of both SFA and MUFA totals than the Atlantic Ocean counterparts (p < 0.05), while the YFT harvested in the Pacific Ocean presented intermediate values, not differing significantly from the other two origins. The YFT from the Indian and Pacific oceans held twice the n-6 PUFA content recorded in the Atlantic YFT (44.1 versus 21.1 mg/100 g of muscle). Considering the recommended daily intake of EPA plus DHA is 250 mg, the consumption of 100 g of YFT from the Atlantic, Indian and Pacific Oceans would provide 149.2 mg, 191.8 mg or 229.6 mg of EPA plus DHA, representing 59.7%, 76.7% or 91.8% of the recommended daily intake, respectively.

Fatty acid ratio analysis identifies changes in competent meroplanktonic larvae sampled over different supply events

Planktonic communities are a cornerstone of ocean food webs. Early benthic performance of meroplanktonic organisms is shaped by their life stages in planktonic communities. Fatty acid profiles of marine invertebrates are a good indicator of their nutritional state and allow inferring how dietary regimes experienced during larval pelagic life may drive their pre- and post-metamorphosis performance. Fatty acid profiles of Carcinus maenas megalopae were analysed during four larval supply events in two consecutive years to better understand the variability in their nutritional state at settlement. The logratio analysis of fatty acids showed differences between the four larval supply events, with five ratios explaining 83.1% of the variance. The ratios that contributed to separate larval supply events presented a combination of essential, de novo synthetized and diet origin fatty acids (e.g., phytanate/20:4 n-6, 16:0/18:2 n-4). The high fatty acid signature dispersion found within the same supply event suggests that larvae settling at Ria de Aveiro (Portugal) developed through different planktonic feeding zones and experienced contrasting feeding regimes. The fatty acid profile of megalopae demonstrated a high contribution of diatoms, flagellates and bacteria in the larval diet of C. maenas. The present study demonstrated differences between supply events, although a high variability of larval phenotypes was recorded within the same supply event.

Assessing the Reliability of Quantitative Fatty Acid Signature Analysis and Compound-Specific Isotope Analysis-Based Mixing Models for Trophic Studies.

The study of the trophic relationships of aquatic animals requires correct estimates of their diets. We compared the quantitative fatty acid signature analysis (QFASA) and the isotope-mixing model IsoError, based on the compound-specific isotope analysis of fatty acids (CSIA-FA), which are potentially effective models for quantitative diet estimations. In a 21-day experiment, Daphnia was fed a mixture of two food items, Chlorella and Cryptomonas, which were supplied in nearly equal proportions. The percentages and isotope values of the FAs of the algal species and Daphnia were measured. The IsoError based on CSIA-FA gave an estimation of algae consumption using only one FA, 18:3n-3. According to this model, the proportion of consumption of Chlorella decreased while the proportion of consumption of Cryptomonas increased during the experiment. The QFASA model was used for two FA subsets—the extended-dietary subset, which included sixteen FAs, and the dietary one, which included nine FAs. According to both subsets, the portion of consumed Chlorella decreased from Day 5 to 10 and then increased at Day 21. The comparison of the two model approaches showed that the QFASA model is a more reliable method to determine the contribution of different food sources to the diet of zooplankton than the CSIA-based mixing model.

Mysterious jellyfish diet gets a fresh look

Jellyfish have been drifting around the planet for more than 500 million years, yet relatively little is known about their lifestyles. Now, scientists at the University of British Columbia’s Institute for the Oceans and Fisheries are using biochemical tools to unravel a key part of the jellyfish mystery: their diet ( J. Exp. Mar. Biol. Ecol. 2021, DOI: 10.1016/j.jembe.2021.151631 ). Scientists frequently use the unique stable isotope and fatty acid signatures present in living things to investigate what predators eat. To calibrate these values for jellyfish, the team, led by Jessica Schaub, performed controlled feeding experiments at the Vancouver Aquarium. Docile moon jellies were fed frozen brine shrimp, which they unexpectedly rejected, and live krill. Voracious Japanese sea nettle jellies were fed frozen brine shrimp and the moon jellies. After analyzing the isotope and fatty acid signatures, the researchers were surprised to find that jellyfish appear to biosynthesize their own

Consistent gut bacterial and short-chain fatty acid signatures in hypoabsorptive bariatric surgeries correlate with metabolic benefits in rats.

The study aimed at comparing how changes in the gut microbiota are associated to the beneficial effects of the most clinically efficient hypoabsorptive bariatric procedures, namely Roux-en-Y gastric bypass (RYGB), biliopancreatic diversion with duodenal switch (BPD-DS) and single anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S). Diet-induced obese (DIO) male Wistar rats were divided into seven groups. In addition to the groups subjected to RYGB, BPD-DS and SADI-S, the following four control groups were included: SHAM-operated rats fed a high-fat diet (SHAM HF), SHAM fed a low-fat diet (SHAM LF), SHAM HF-pair-weighed to BPD-DS (SHAM HF-PW) and sleeve-gastrectomy (SG) rats. Body weight, food intake, glucose tolerance, insulin sensitivity/resistance, and L-cell secretion were assessed. The gut microbiota (16 S ribosomal RNA gene sequencing) as well as the fecal and cæcal contents of short-chain fatty acids (SCFAs) were also analyzed prior to, and after the surgeries. The present study demonstrates the beneficial effect of RYGB, BPD-DS and SADI-S on fat mass gain and glucose metabolism in DIO rats. These benefits were proportional to the effect of the surgeries on food digestibility (BPD-DS > SADI-S > RYGB). Notably, hypoabsorptive surgeries led to consonant microbial signatures characterized by decreased abundance of the Ruminococcaceae (Oscillospira and Ruminococcus), Oscillospiraceae (Oscillibacter) and Christensenellaceae, and increased abundance of the Clostridiaceae (Clostridium), Sutterellaceae (Sutterella) and Enterobacteriaceae. The gut bacteria following hypoabsorptive surgeries were associated with higher fecal levels of propionate, butyrate, isobutyrate and isovalerate. Increases in the fecal SCFAs were in turn positively and strongly correlated with the levels of peptide tyrosine-tyrosine (PYY) and with the beneficial effects of the surgery. The present study emphasizes the consistency with which the three major hypoabsorptive bariatric procedures RYGB, BPD-DS and SADI-S create a gut microbial environment capable of producing a SCFA profile favorable to the secretion of PYY and to beneficial metabolic effects.

Assessment of Arabian Gulf Seaweeds from Kuwait as Sources of Nutritionally Important Polyunsaturated Fatty Acids (PUFAs).

The fatty acid (FA) compositions of ten seaweeds representative of Chlorophyta, Rhodophyta, and Ochrophyta from Kuwait in the Arabian Gulf region were determined and are discussed in the context of their potential nutritional perspectives for seaweed valorization. All the seaweeds had higher saturated fatty acid (SFA) and lower monounsaturated (MUFA) and polyunsaturated fatty acid (PUFA) contents than those typical of tropical environments. Palmitic, myristic, stearic, oleic, linoleic, α-linolenic, and stearidonic acids were the major FAs detected. Arachidonic, eicosapentaenoic, and docosahexaenoic acids were detected in minor amounts. Conserved fatty acid patterns revealed phylogenetic relationships among phyla, classes, and orders matching the molecular phylogenies at higher taxonomic ranks. Hierarchical clustering analyses clearly segregated different seaweeds (except Codium papillatum and Iyengaria stellata) into distinct groups based on their FA signatures. All but one species (Chondria sp.) had health-beneficial n6/n3 PUFAs (0.33:1–2.94:1) and atherogenic (0.80–2.52) and thrombogenic indices (0.61–5.17). However, low PUFA/SFA contents in most of the species (except Ulva spp.) may limit their utilization in the formulation of PUFA-rich functional foods. Ulva spp. had substantially high PUFAs with PUFA/SFA > 0.4, n6/n3 (0.33–0.66) and atherogenic (0.80–1.15) and thrombogenic indices (0.49–0.72), providing substantial potential for their utilization in food and feed applications.

Polar bear diet composition reveals spatiotemporal distribution of Arctic marine mammals across Nunavut, Canada

Climate warming and associated physical and biological changes will likely force widespread species redistribution, particularly in polar environments. However, tracking such distributional shifts is difficult. The dietary habits of apex predators, like polar bears (Ursus maritimus), may provide early signals of distributional change in prey populations. We used harvest-based sampling to investigate the spatial feeding patterns of polar bears across Nunavut from 2010 to 2018 (n = 1570) and identify spatiotemporal clusters of different prey based on predator diet estimates. Quantitative fatty acid signature analysis and the Getis-Ord Gi* statistic identified spatial clusters of high or low dietary proportions (i.e., “hot spots” and “cold spots”) reflecting seasonal and spatial availability of prey. Ringed seal (Pusa hispida) was the primary prey of bears throughout Nunavut followed by bearded seal (Erignathus barbatus), although proportional consumption varied spatially. A consistent ringed seal consumption hot spot was found in Gulf of Boothia indicating the importance of year-round availability of ringed seals. Spatial clusters of bearded seal and Atlantic walrus (Odobenus rosmarus rosmarus) throughout Foxe Basin suggested overlapping seasonal distributions and high regional abundance. Bears had consistently high dietary levels of harbour seal (Phoca vitulina) around Southampton Island and along the western coast of Hudson Bay suggesting a possible year-round concentration of this prey. Hot spots of harp seal (Pagophilus groenlandicus) consumption were evident throughout Davis Strait and a spring-summer hot spot around Jones Sound was consistent with harp seal migratory patterns. Year-round beluga whale (Delphinapterus leucas) hot spots were found along eastern Baffin Island and southern Viscount Melville Sound providing new knowledge of local conditions that promote polar bear predation or scavenging. Narwhal (Monodon monoceros) were less susceptible to predation with only one spatial cluster of high consumption appearing during spring-summer in Barrow Strait. Bowhead whale (Balaena mysticetus) hot spots occurred around south-western Foxe Basin and seasonally in southern Viscount Melville Sound suggesting carcasses are locally accessible to bears and may act as a supplemental food source in particular areas and seasons. The congruence of polar bear feeding habits and known prey distribution suggests polar bears serve as ecological indicators and ongoing monitoring of their diets may reveal regional and broad-scale changes in prey population distributions and Arctic ecosystem functioning.

Fatty acid signatures of sediment microbial community in the chronically polluted mangrove ecosystem

Phospholipid fatty acid (PLFA) analysis was used to examine variation in the distribution of microbial communities in heavily polluted mangrove sediments of Thane creek, west coast of India. A total of 40 individual PLFAs representing 11 functional groups were identified in the sediment and were mainly dominated by saturated fatty acids (anaerobic prokaryotes) >50%. Significant dominance of PUFA, 16:3 ω6c (34.2%) indicators of micro-eukaryotes, in subsurface depth (p < 0.05) suggests input from the remnants of marine microalgae. Declined mean relative abundance of fungi (<6%) and actinomycetes (<1%) were detected in the sediment indicating their sensitivity to anthropic stressors. Homogenous profile of microbial diversity indicating active bioturbation. Cumulative metabolic stress evident from SAT/MUFA (>1), B/F (>1) and G+/G– (<1) ratio and prolonged hypoxia to be prevalent in the creek during the study. In conclusion, PLFA signatures can thus be used as potential biomarkers of environmental monitoring and proxy for interpreting ecosystem health.

Individual and population dietary specialization decline in fin whales during a period of ecosystem shift

This study sought to estimate the effect of an anthropogenic and climate-driven change in prey availability on the degree of individual and population specialization of a large marine predator, the fin whale (Balaenoptera physalus). We examined skin biopsies from 99 fin whales sampled in the St. Lawrence Estuary (Canada) over a nine year period (1998–2006) during which environmental change was documented. We analyzed stable isotope ratios in skin and fatty acid signatures in blubber samples of whales, as well as in seven potential prey species, and diet was quantitatively assessed using Bayesian isotopic models. An abrupt change in fin whale dietary niche coincided with a decrease in biomass of their predominant prey, Arctic krill (Thysanoessa spp.). This dietary niche widening toward generalist diets occurred in nearly 60% of sampled individuals. The fin whale population, typically composed of specialists of either krill or lipid-rich pelagic fishes, shifted toward one composed either of krill specialists or true generalists feeding on various zooplankton and fish prey. This change likely reduced intraspecific competition. In the context of the current “Atlantification” of northern water masses, our findings emphasize the importance of considering individual-specific foraging tactics and not only population or group average responses when assessing population resilience or when implementing conservation measures.