Signal Level Research Articles

NOE analysis using dual injection DNP‐NMR for studies of solvent–solute interactions at low concentrations

AbstractDynamic nuclear polarization (DNP) offers significant signal enhancement compared to conventional nuclear magnetic resonance (NMR) in measuring the nuclear Overhauser effect. Owing to this enhancement, DNP enables the quantitative analysis of solvent–solute interactions, which are typically challenging to assess using standard NMR techniques. However, current experimental setups generally require the use of samples with relatively high solute concentrations because of large dilution factors. This limits the studies on solvent–solute interactions for bio‐related molecules, particularly those with low solubility. In such cases, the concentration of solute post‐dilution often falls below the detection limits. Herein, we introduce a novel dual injection system designed to considerably increase the dilution factor, thereby enabling the use of samples with lower solute concentrations while maintaining detectable signal levels. The effectiveness of this system was demonstrated in experiments using trifluoroacetic acid at concentrations 50 times lower than those used in the conventional method.

Submissive behaviour is affected by group size in a social fish.

For social groups to form and be stable over time, animals must develop strategies to cope with conflict among group members. Animals may behave submissively either by fleeing from an aggressor, or by signalling submission. The use of these two submissive responses may vary depending on the social and ecological context. Group size is a key aspect of social context for group living animals, as individuals in smaller groups may respond to aggression differently than those from larger groups. Here, we examine the relationship between group size and submissive behaviour in a cooperatively breeding fish, the daffodil cichlid (Neolamprologus pulcher). We found that subordinate fish showed similar levels of submission signals in response to dominant aggression in larger and smaller groups, however, subordinates from larger groups were less likely to flee from dominant aggression than those in smaller groups. Subordinates in larger groups also showed more digging behaviour which may be also used to avoid conflict with the dominant group members. Our data show that social context affects submissive behaviour in a cooperatively breeding fish.

Abstract 2027: Enhancing Cell-based Therapies With Peptide-modified Thermoresponsive Citrate-based Biomaterials For Treatment Of Critical Limb Ischemia

Introduction: Critical limb ischemia (CLI) causes severe morbidity and mortality with limited treatment options. Cell-based therapy has potential to advance treatment outcomes but faces challenges in cell survival and engraftment during delivery. We hypothesize that an antioxidant niche that promotes cell adhesion and spreading will improve cell engraftment and survival rate, with the goal of limb salvage through improved perfusion. Methods: Thermoresponsive poly(polyethylene glycol citrate-co-N-isopropylacrylamide) (PPCN) was synthesized and conjugated with laminin-derived (A5G81) or VEGF-mimic (QK) peptides. Human umbilical vein endothelial cells (HUVECs) expressing human sodium iodine symporter (hNIS) were mixed with materials and intramuscularly injected into nude mice hindlimbs and monitored with Single Photon Emission Computed Tomography with Computed Tomography (SPECT/CT) for cell survival. Next, a hindlimb ischemia model was used to assess the efficacy of cell delivery by evaluating limb perfusion, tissue loss, functional impairment and histological examination. Results: Cells delivered with PBS or PPCN demonstrated poor cell survival, with over 90% cell loss by day 7 ( Figure 1a ). In contrast, cells delivered with PPCN-A5G81 and PPCN-QK exhibited robust SPECT signal levels for up to four weeks ( Figure 1a,b ). In the hindlimb ischemia model, materials or cells alone did not demonstrate improvement in any of the parameters over the PBS control. However, cells delivered with PPCN-A5G81 and PPCN-QK showed significantly higher blood perfusion ( Figure 1c-e ), with improved tissue salvage and motor functions ( Figure 1f-i ) for both male and female mice. Conclusion: PPCN modified with A5G81 and QK promotes cell spreading, proliferation, survival, and tissue regeneration, with potential for more effective cell-based therapies for CLI.

Investigating the Utility of a Compact Loudspeaker Array for Audiometric Testing.

This project addressed the uses of a loudspeaker array for audiometric measurements. It sought to evaluate a prototype compact array in terms of the reliability of test results across sound booths. A seven-loudspeaker array was developed to deliver sounds from -60° to +60° on an arc with a radius of 0.5 m. The system was equipped with a head position sensing system to maintain the listener's head near the optimal test position. Three array systems were distributed to each of the two test sites for within-subject assessments of booth equivalence on tests of sound localization, speech reception in noise, and threshold detection. A total of 36 subjects participated, 18 at each test site. Results showed excellent interbooth consistency on tests of sound localization using speech and noise signals, including conditions in which one or both ears were covered with a muff. Booth consistency was also excellent on sound field threshold measurements for detecting quasi-diffuse noise bands. Nonequivalence was observed in some cases of speech-in-noise tests, particularly with a small one-person booth. Acoustic analyses of in situ loudspeaker responses indicated that some of the nonequivalent comparisons on speech-in-noise tests could be traced to the effects of reflections. Overall, the results demonstrate the utility and reliability of a compact array for the assessment of localization ability, speech reception in noise, and sound field thresholds. However, the results indicate that researchers and clinicians should be aware of the reflection effects that can influence the results of sound field tests in which signal and noise levels from separate loudspeakers are critical.

Effectiveness of Yijinjing on cognitive and motor functions in patients with Parkinson's disease: study protocol for a randomized controlled trial.

Parkinson's disease (PD) is a common neurodegenerative disorder that affects motor and non-motor functions, significantly reducing patients' quality of life. No effective drug-based treatments are known to solve this problem. Non-drug therapies such as Yijinjing exercise have shown potential in improving cognitive and motor functions in PD patients. However, solid evidence must still be provided to support their clinical efficacy. This study aims to evaluate the clinical efficacy of Yijinjing exercise interventions in PD patients and explore the underlying mechanisms between the cognitive and motor functions in PD. This is a single-center randomized controlled trial in which 96 eligible PD patients will be randomly assigned to receive either Yijinjing exercise group or brisk walking group or control group in a ratio of 1:1:1. Interventions (Yijinjing exercise or brisk walking training, 40 min per session) will be provided in 3 sessions per week (Monday, Wednesday, Friday) for 12 weeks, with a total of 36 sessions. After the treatment, there will be a 1-month follow-up period. The primary outcomes will be measured using the Montreal Cognitive Assessment (MoCA) and the Unified Parkinson's Disease Rating Scale motor section (UPDRS-III). Secondary outcomes include balance function, executive function, walking function, sleep quality, and quality of life. Additionally, the prefrontal cerebral and sensorimotor cortex blood oxygen signal level will be collected to explore the underlying mechanisms. All outcomes will be assessed at baseline, at the end of 12 weeks of treatment and after an additional 1-month follow-up period. The results of the study protocol will provide high-quality evidence for the potential of intervention measures based on the Yijinjing exercise to improve the cognitive and activity levels of Parkinson's disease patients. We envision the Yijinjing exercise as a non-pharmacological family activity that can provide a new and more effective method for the treatment of Parkinson's disease patients or those at risk. This study was approved by the Ethics Committee of the Second Rehabilitation Hospital of Shanghai (2020-05-01). The trial has been registered in the China Clinical Trials Registry (ChiCTR2200055636).

Isoglutaminyl Cyclase Overexpression Enhances KYSE30 Cancer Cell Proliferation and Migration via the MAPK Signaling Pathway.

To understand how upregulated isoglutaminyl cyclase (isoQC) is involved in the initiation of diseases such as cancer, we developed a human KYSE30 carcinoma cell model in which isoQC was stably overexpressed. GO and KEGG analysis of the DEGs (228) and DEPs (254) respectively implicated isoQC on the proliferation invasion and metastasis of cells and suggested that isoQC might participate in the regulation of MAPK, RAS, circadian rhythm, and related pathways. At the functional level, isoQC-overexpressing KYSE30 cells showed enhanced proliferation, migration, and invasion capacity. Next, we decided to study the precise effect of isoQC overexpression on JNK, p-JNK, AKT, p-AKT, ERK, p-ERK, and PER2, as RNA levels of these proteins are significantly correlated with signal levels indicated in RNA-Seq analysis, and these candidates are the top correlated DEPs enriched in RT-qPCR analysis. We saw that only p-ERK expression was inhibited, while PER2 was increased. These phenotypes were inhibited upon exposure to PER2 inhibitor KL044, which allowed for the restoration of p-ERK levels. These data support upregulated isoQC being able to promote cancer cell proliferation and migration in vitro, likely by helping to regulate the MAPK and RAS signaling pathways, and the circadian protein PER2 might be a potential mediator.

An ANALYSIS OF DVB-T2 TV BROADCAST TESTING DRIVE TEST AND FIELD STRENGTH METHODS IN THE JAKARTA SERVICE AREA

Indonesia has adopted DVB-T2 as the technology standard for terrestrial digital television broadcasting. This research aims to test and analyze the reception quality of DVB-T2 TV broadcasts in the DKI Jakarta service area. The research was conducted using drive test and field strength methods to test and evaluate the quality of digital television broadcasts. Tests were conducted in various locations in the DKI Jakarta service area using the Gsertel Hexylon TV Analyzer technical device to test DVB-T2 TV broadcast signals. The field strength test results were more accurate and higher values were generated than the drive test. Several parameters such as signal level and MER are evaluated and analyzed to find out what affects their values. The transmitter parameter settings such as modulation, FEC code rate, FFT Mode, Guard Interval, and Pilot Pattern greatly affect the signal level and MER values. The optimization performed on CH 24 also managed to maintain its signal level value of 18.19 dB and its MER of 55.01 dBµV to maintain broadcast stability during a technical failure of the transmitter on CH 1.

Microwave re-excitation of femtosecond laser tagging for highly flexible velocimetry.

Molecular tagging velocimetry is typically species specific and limited by excited state/species lifetimes. We utilize laser-generated ionization, long-lived anions, and a time-delayed microwave pulse to monitor the tagged region up to several milliseconds. This non-resonant excitation and microwave interaction is demonstrated in a range of gas mixtures. Signal levels show up to 1000-fold improvement, and the flexibility in interrogation time allows for velocity measurements over a large dynamic range (1-100 m/s) with single-shot precision of <5%. This approach has the potential for wide application over a range of relevant gas compositions, temperatures, and pressures.

Tumor phylogeography reveals block-shaped spatial heterogeneity and the mode of evolution in Hepatocellular Carcinoma

Solid tumors are complex ecosystems with heterogeneous 3D structures, but the spatial intra-tumor heterogeneity (sITH) at the macroscopic (i.e., whole tumor) level is under-explored. Using a phylogeographic approach, we sequence genomes and transcriptomes from 235 spatially informed sectors across 13 hepatocellular carcinomas (HCC), generating one of the largest datasets for studying sITH. We find that tumor heterogeneity in HCC segregates into spatially variegated blocks with large genotypic and phenotypic differences. By dissecting the transcriptomic heterogeneity, we discover that 30% of patients had a “spatially competing distribution” (SCD), where different spatial blocks have distinct transcriptomic subtypes co-existing within a tumor, capturing the critical transition period in disease progression. Interestingly, the tumor regions with more advanced transcriptomic subtypes (e.g., higher cell cycle) often take clonal dominance with a wider geographic range, rejecting neutral evolution for SCD patients. Extending the statistical tests for detecting natural selection to many non-SCD patients reveal varying levels of selective signal across different tumors, implying that many evolutionary forces including natural selection and geographic isolation can influence the overall pattern of sITH. Taken together, tumor phylogeography unravels a dynamic landscape of sITH, pinpointing important evolutionary and clinical consequences of spatial heterogeneity in cancer.

Do government performance signals affect citizen satisfaction?

AbstractPrevious studies have confirmed the causal effect of performance information on citizen satisfaction, but they were primarily conducted in survey experimental settings that featured hypothetical and abstract scenarios and primed respondents to look at certain aspects of performance information. Whether the causal effects hold in the real world, which is a much more complex information environment, is questionable. We address the gaps by employing a regression discontinuity design to identify the impact of public schools’ performance grades on parents’ satisfaction with teachers and overall education in New York City. We find that performance signals have independent and lasting effects on citizens’ satisfaction. However, the effects are nonlinear, depending on the levels of performance signals. Parents’ responses are muted at the A/B performance grade cutoff, but their satisfaction increases significantly at the B/C and C/D cutoffs if their schools earn relatively higher grades.

Network pharmacology and experimental evidence: ERK/CREB/BDNF signaling pathway is involved in the antidepressive roles of Kaiyu Zhishen decoction

Ethnopharmacological relevanceMajor Depressive Disorder (MDD) emerges as a complex psychosomatic condition, notable for its considerable suicidality and mortality rates. Increasing evidence suggests the efficacy of Chinese herbal medicine in mitigating depression symptoms and offsetting the adverse effects associated with conventional Western therapeutics. Notably, clinical trials have revealed the adjunctive antidepressant potential of Kaiyu Zhishen Decoction (KZD) alongside Western medication. However, the standalone antidepressant efficacy of KZD and its underlying mechanisms merit in-depth investigation. Aim of the studyThis research aims to elucidate the impact of KZD on MDD and delineate its mechanistic pathways through integrated network pharmacological assessments and empirical in vitro and in vivo analyses. Materials and methodsTo ascertain the optimal antidepressant dosage and mechanism of KZD, a Chronic Unpredictable Mild Stress (CUMS)-induced depression model in mice was established to evaluate depressive behaviors. High-Performance Liquid Chromatography (HPLC) and network pharmacological approaches were employed to predict KZD's antidepressant mechanisms. Subsequently, hippocampal samples were subjected to 4D-DIA proteomic sequencing and validated through Western blot, immunofluorescence, Nissl staining, and pathway antagonist applications. Additionally, cortisol-stimulated PC12 cells were utilized to simulate neuronal damage, analyzing protein and mRNA levels of MAPK-related signals and cell proliferation markers. ResultsThe integration of network pharmacology and HPLC identified kaempferol and quercetin as KZD's principal active compounds for MDD treatment. Proteomic and network pharmacological KEGG pathway analyses indicated the MAPK signaling pathway as a critical regulatory mechanism for KZD's therapeutic effect on MDD. KZD was observed to mitigate CUMS-induced upregulation of p-ERK/ERK, CREB, and BDNF protein expressions in hippocampal cells by attenuating oxidative stress, thereby ameliorating neuronal damage and exerting antidepressant effects. The administration of PD98059 counteracted KZD's improvements in depression-like behaviors and downregulated p-ERK/ERK and BDNF protein expressions in the hippocampus. ConclusionsThis investigation corroborates KZD's pivotal, dose-dependent role in antidepressant activity. Both in vivo and in vitro experiments demonstrate KZD's capacity to modulate the ERK-CREB-BDNF signaling pathway by diminishing ROS expression induced by oxidative stress, enhancing neuronal repair, and thus, manifesting antidepressant properties. Accordingly, KZD represents a promising herbal candidate for further antidepressant research.

An "All-In-One" Immunomodulator-Engineered Clinical Translatable Immunotherapy of Advanced Hepatocellular Carcinoma.

Clinical treatment of advanced hepatocellular carcinoma (HCC) remains a significant challenge. Utilizing1-bromoacetyl-3,3-dinitroazetidine (RRx-001) to downregulate the expression of innate immune checkpoint molecule, cluster of differentiation 47 (CD47), provides a powerful means for treating advanced HCC containing abundant immunosuppressive macrophages. Herein engineering of a previously optimized Doxorubicin (DOX)-delivery nanoplatform based on sodium alginate is reported to further co-deliver RRx-001 (biotinylated aldehyde alginate-doxorubicin micelle prodrug nanoplatform, BEA-D@R) for efficient immunotherapy of advanced HCC. Thisgroundbreaking technique reveals the "all-in-one" immunotherapeutic functionalities of RRx-001. Besides the previously demonstrated functions of downregulating CD47 expression and increasing reactive nitrogen species (RNS) generation, another key function of RRx-001 for downregulating the expression of the adaptive immune checkpoint molecule programmed cell death 1 ligand 1 (PDL1) is first uncovered here. Combined with the reactive oxygen species (ROS) generation and an upregulated "eat me" signal level of DOX, BEA-D@R collectively increases RNS generation, enhances T-cell infiltration, and maximizes macrophage phagocytosis, leading to an average of 40% tumor elimination in a mice model bearing an initial tumor volume of ≈300 mm3 that mimics advanced HCC. Overall, the "all-in-one" immunotherapeutic functionalities of a clinical translatable nanoplatform are uncovered for enhanced immunotherapy of advanced HCC.

400 Brain AVMs-Related MicroRNAs: Machine Learning Algorithm for Expression Profiles of Target Genes

INTRODUCTION: microRNAs (miRNAs) are a class of non-coding RNAs playing a myriad of important roles in regulating gene expression. Of note, recent work demonstrated a critical role of miRNAs in the genesis and progression of brain arteriovenous malformations (bAVMs). METHODS: A PRISMA-based literature review was performed using PubMed/Medline database with the following search terms: "brain arteriovenous malformations", "cerebral arteriovenous malformations", "microRNA", and "miRNA". All preclinical and clinical studies written in English, regardless of date, were selected. For our bioinformatic analyses, miRWalk and miRTarBase machine learning algorithms were employed; the Kyoto Encyclopedia of Genes and Genomes (KEGG) database was quired for associated pathways/functions. RESULTS: 4 studies were ultimately included in the final analyses. Sequencing data consistently revealed the decreased expression of miR-18a in bAVM-endothelial cells, resulting in increased levels of vascular endodermal growth factor (VEGF), Id-1, matrix metalloproteinase, and growth signals. Our analyses also suggest that the downregulation of miR-137 and miR-195* within vascular smooth muscle cells (VSMCs) may foster the activation of inflammation, aberrant angiogenesis, and phenotypic switching. In the peripheral blood, the overexpression of miR-7-5p, miR-629-5p, miR-199a-5p, miR-200b-3p, and let-7b-5p may contribute to endothelial proliferation and nidus development. The machine learning algorithms employed confirmed associations between miRNA-related target networks, vascular rearrangement, and bAVM progression. CONCLUSIONS: miRNAs expression appears to be critical in managing bAVMs' post-transcriptional signals. Targets of microRNAs regulate canonical vascular proliferation and reshaping. Although additional scientific evidence is needed, the identification of bAVM miRNA signatures may facilitate the development of novel prognostic/diagnostic tools and molecular therapies for bAVMs.

Ultracompact Lab-on-a-Chip Device for Surface-Enhanced Coherent Anti-Stokes Raman Scattering.

This paper introduces an ultracompact lab-on-a-chip device with a size of [Formula: see text] for surface-enhanced coherent anti-Stokes Raman scattering. This device comprises of a unique hybrid plasmonic-photonic vertical coupler, for light-coupling between the device and a light source, and a heptamer plasmonic nanotweezer for trapping and manipulation of nanoparticles. The coupler with its nanoscale size of [Formula: see text] offers maximum coupling efficiency and directivity of -4.2 dB and 17.8 dB with a 3 dB bandwidth across the wavelength range of [Formula: see text]. Based on a finite element method, it is theoretically shown that the tear-drop based nanotweezer can empower the device to yield a very high Raman gain of ≥ 1017 , making the detection at the single-molecule level possible. The proposed device can exhibit low damage to bioparticles through the use of near infrared wavelengths range with lower energy photons. Due to its short optical path, this device is expected to improve the Raman signal to noise ratio by reducing the level of background in-coherent Raman signals. Finally, thanks to its configuration, this device can enable the creation of parallel measurement channels, paving the way toward the development of high-throughput and mass-produced biosensors.

Mitigation of the Brighter-fatter Effect in the LSST Camera

Thick, fully depleted charge-coupled devices are known to exhibit nonlinear behavior at high signal levels due to the dynamic behavior of charges collecting in the potential wells of pixels, called the brighter-fatter effect (BFE). The effect results in distorted images of bright calibration stars, creating a flux-dependent point-spread function that if left unmitigated, could make up a large fraction of the error budget in Stage IV weak-lensing (WL) surveys such as the Legacy Survey of Space and Time (LSST). In this paper, we analyze image measurements of flat fields and artificial stars taken at different illumination levels with the LSST Camera (LSSTCam) at SLAC National Accelerator Laboratory in order to quantify this effect in the LSSTCam before and after a previously introduced correction technique. We observe that the BFE evolves anisotropically as a function of flux due to higher-order BFEs, which violates the fundamental assumption of this correction method. We then introduce a new method based on a physically motivated model to account for these higher-order terms in the correction, and then we test the modified correction on both data sets. We find that the new method corrects the effect in flat fields better than it corrects the effect in artificial stars, which we suggest is the result of sub-pixel physics not included in this correction model. We use these results to define a new metric for the full-well capacity of our sensors and advise image processing strategies to further limit the impact of the effect on LSST WL science pathways.

Safety hysteresis comparator design for transient overvoltage detection

This research introduces a safety hysteresis comparator that detects transient overvoltage in the track circuit relay interlocking of railway signaling system. This overvoltage is caused by voltage faults transmitted through the electric conductor on the track feed unit to the receiver equipped with the track relay, which acts as the occupied track circuit controller. The circuit was designed using safety system design principles and concepts. Findings illustrated that the transient overvoltage detection of the safety hysteresis comparator in the track circuit activated when the input voltage (Vin) was higher than the high hysteresis signal level (Vhyst_H). When Vin was less than low hysteresis signal level (VVhyst_L), the output voltage (Vo) state was low. Otherwise, it was high. The hysteresis voltage was 4.4 V. The installation of the transient overvoltage detector in the track circuit was to monitor the transient overvoltage fault in the track circuit and to confirm that the hysteresis comparator was in the safety failure mode, which was the safety function for the track circuit to be compliant with the IEC 61800-5-2 standard to ensure the system stability and reliability. It would maximize the performance of the controlling function and commanding of train arrangement of State Railway of Thailand (SRT).

Characterization and Optimization of Skipper CCDs for the SOAR Integral Field Spectrograph

We present results from the characterization and optimization of Skipper charge-coupled devices (CCDs) for use in a focal plane prototype for the Southern Astrophysical Research Integral Field Spectrograph (SIFS). We tested eight Skipper CCDs and selected six for SIFS based on performance results. The Skipper CCDs are 6k × 1k, 15 μm pixels, thick, fully depleted, p-channel devices that have been thinned to ∼250 μm, backside processed, and treated with an anti-reflective coating. We demonstrate a single-sample readout noise of <4.3 e− rms pixel−1 in all amplifiers. We optimize the readout sequence timing to achieve a readout noise of 0.5 e− rms pixel−1 after 74 non-destructive measurements, which can be accomplished in a region covering 5% of the detector area in a readout time of <4 minutes. We demonstrate single-photon-counting in all 24 amplifiers (four amplifiers per detector) with a readnoise of σ N ∼ 0.18 e− rms pixel−1 after N samp = 400 samples, and we constrain the degree of nonlinearity to be ≲1% at low signal levels (0 e− to 50 e−). Clock-induced charge (CIC) remains an important issue when the Skipper CCD is configured to provide a large full-well capacity. We achieve a CIC rate of <1.45 × 10−3 e− pixel−1 frame−1 for a full-well capacity of ∼900 e−, which increases to a CIC rate of ∼3 e− pixel−1 frame−1 for full-well capacities ∼40,000–65,000 e−. We also perform conventional CCD characterization measurements such as charge transfer inefficiency (3.44 × 10−7 on average), dark current (∼2 × 10−4 e− pixel−1 s−1), photon transfer curves, cosmetic defects (<0.45% “bad” pixels), and charge diffusion (point-spread function < 7.5 μm) to verify that these properties are consistent with expectations from conventional p-channel CCDs used for astronomy. Furthermore, we provide the first measurements of the brighter-fatter effect and absolute quantum efficiency (≳80% between 450 and 980 nm; ≳90% between 600 and 900 nm) using Skipper CCDs.

Prediction of Radio Signal Failures of Communication Based Train Operating Systems by Machine Learning Methods

The use of rail systems in urban public transportation has become a necessity for reasons such as time saving, travel comfort and operating costs, especially in cities with high population and road traffic. Communication based train operating systems (CBTC) are used for the safe use of urban rail systems and the maximum capacity of the railway line. In this study, in line with the data collected from the trains on a railway line operated with CBTC, the status of the radio signals that enable the wireless communication of the trains with the trackside signaling equipment was evaluated by machine learning methods, and the situations that may have negative effects on the train operations of the problems at the signal level were evaluate. The problems on the antennas which receives signals from trackside above trains, the poor connection related with fiber optical and LAN cables, the trackside transmitter antenna orientation problems causes decrease on signal levels. It is aimed that to take actions about the problematic signal levels without any negative impact on the passenger comfort and the operation yet. The radio signal losses cause unexpected trains stops and delays. A decision support model has been developed that will offer early solution suggestions to system maintainers in order to intervene first. In conclusion, since it is the first study related with failure prediction by using radio signal levels data on railway signaling system, this study presents an important innovation in terms of literature.

Analysis and Simulation of Current Balancer Circuit for Phase-Gain Correction of Unbalanced Differential Signals

The phase and gain imbalance of a balun output can be adjusted by a differential current balancer (DCB) circuit. The performance of DCB circuit, for correcting the phase (gain) imbalance, is analyzed for a wide range of input signal level, and the accuracy is verified with circuit simulation. To illustrate the phase-error/gain-error (PE/GE) correction, a 30–40[Formula: see text]GHz DCB circuit is designed and simulated in a 180-nm CMOS process. The DCB is examined for input PE, [Formula: see text], of [Formula: see text] and input GE, GA, of [Formula: see text][Formula: see text]dB. Analysis and simulation illustrate an output phase error [Formula: see text] of [Formula: see text] and output gain error [Formula: see text] of [Formula: see text][Formula: see text]dB, over frequency range of 20–50[Formula: see text]GHz. The results of DCB circuit for PE (GE) compensation are compared to that of phase-correction technique (PCT) circuit, illustrating the superior phase (gain) imbalance correction of the DCB circuit with lower NF and DC power consumption.

Performance Enhancement in a Few-Mode Rayleigh-Brillouin Optical Time Domain Analysis System Using Pulse Coding and LMD Algorithm

Rayleigh Brillouin optical time domain analysis (BOTDA) uses the backscattered Rayleigh light generated in the fiber as the probe light, which has a lower detection light intensity compared to the BOTDA technique. As a result, its temperature-sensing technology suffers from a low signal-to-noise ratio (SNR) and severe sensing unreliability due to the influence of the low probe signal and high noise level. The pulse coding and LMD denoising method are applied to enhance the performance of the Brillouin frequency shift detection and temperature measurement. In this study, the mechanism of Rayleigh BOTDA based on a few-mode fiber (FMF) is investigated, the principles of the Golay code and local mean decomposition (LMD) algorithm are analyzed, and the experimental setup of the Rayleigh BOTDA system using an FMF is constructed to analyze the performance of the sensing system. Compared with a single pulse of 50 ns, the 32-bit Golay coding with a pulse width of 10 ns improves the spatial resolution to 1 m. Further enhanced by the LMD algorithm, the SNR and temperature measurement accuracy are increased by 5.5 dB and 1.05 °C, respectively. Finally, a spatial resolution of 1.12 m and a temperature measurement accuracy of 2.85 °C are achieved using a two-mode fiber with a length of 1 km.