Signs Of Deficiency Research Articles

Non-ceruloplasmin copper and urinary copper in clinically stable Wilson disease: Alignment with recommended targets

Background and AimsWilson disease (WD) is caused by accumulation of copper primarily in liver and brain. During maintenance therapy of WD with D-penicillamine, current guidelines recommend on-treatment ranges of urinary copper excretion (UCE) 200-500 μg/24h and serum non-ceruloplasmin bound copper (NCC) 50-150 μg/L. We compared NCC measured by two novel assays and UCE from clinically stable WD patients on D-penicillamine therapy with these recommendations. MethodsThis is a secondary analysis of data from the Chelate Trial (NCT03539952) that enrolled physician selected clinically stable WD patients on D-penicillamine maintenance therapy with unaltered dose for at least four months. We analyzed laboratory samples from the first screening visit, prior to interventions. NCC was measured by either protein speciation (NCC-Sp) using anion exchange HPLC protein speciation followed by copper determination with ICP-MS or as exchangeable copper (NCC-Ex). NCC-Sp was also analyzed in healthy controls (n=75). ResultsIn 76 WD patients with 21.3±14.3 average treatment-years, NCC-Sp (Mean±SD: 56.6±26.2 μg/L) and NCC-Ex (Mean±SD: 57.9±24.7 μg/L) were within the 50-150 μg/L target in 61% and 54%, respectively, below in the remaining. In addition, 36% and 31%, respectively, were even below the normal ranges (NCC-Sp: 46-213 μg/L, NCC-Ex: 41-71 μg/L). NCC-Ex positively correlated with NCC-Sp (r2=0.66, P<0.001) but with systematic deviation. UCE was outside the 200-500 μg/24H target in 58%. Only 14/69 (20%) fulfilled both the NCC-Sp and UCE targets. Clinical or biochemical signs of copper deficiency were not detected. ConclusionClinically stable WD patients on maintenance D-penicillamine therapy frequently have lower NCC-Sp or higher UCE than current recommendations without signs of overtreatment. Further studies are warranted to identify appropriate target ranges of NCC-Sp, NCC-Ex and UCE in treated WD. Impact and implicationsChelator treatment of patients with Wilson Disease (WD) is currently guided by measurements of non-ceruloplasmin bound copper (NCC) and 24 h urinary copper excretion (UCE) but validation is limited. In 76 adults with ≈21 years history of treated WD and clinically stable disease on D-penicillamine therapy, NCC was commonly found to be below normal values and recommended target ranges whether measured by protein speciation (NCC-Sp) or as exchangeable copper (NCC-Ex) while UCE values were above recommended target range in 49%. Common wisdom would suggest overtreatment in these cases but no clinical or biochemical signs of copper deficiency were observed. Exploratory analysis of liver enzymes suggested that NCC below levels seen in controls may be beneficial, while the relation to UCE was less clear. The data calls for critical re-evaluation of target ranges for treatment of WD, specific for drug and laboratory methodology. Clinical trial number(NCT03539952)

EVALUATION OF PHYSICAL DEVELOPMENT IN CHILDREN WITH BRONCHIAL ASTHMA

Among the diseases capable of negatively affecting the course of bronchial asthma in children and adolescents, disruptions in nutritional status play an important role, including not only excess body weight and obesity, but also insufficient body weight. Since children with the consequences of perinatal central nervous system damage often exhibit signs of protein-energy deficiency, the aim of the research conducted was to assess the indicators of nutritional status of children with bronchial asthma depending on accompanying neurological symptoms by evaluating the body mass index. 126 children with bronchial asthma were examined, divided into two subgroups: the main group included 65 children (51.6%) with the disease occurring against the background of consequences of perinatal central nervous system damage; the comparison group included 61 patients (48.4%) without accompanying neurological symptoms. The average age of the children was 11.4±2.9 years. According to the results obtained, in 50.8% of observations, children in the main group had insufficient body weight and a decrease in body mass index, while in the comparison group, excess body weight was observed in 37.7% of cases. KEYWORDS: children, bronchial asthma, anthropometry, body mass index, nutritional status, central nervous system

Smart Agriculture Automation System using ML

Abstract: The "Smart Agriculture Automation System using Machine Learning" project envisages a future where farming transcends manual labor and embraces data-driven automation. In response to the escalating demand for food amidst a burgeoning global population, traditional farming methods are rendered insufficient. However, Smart Agriculture Automation Systems offer a transformative solution by enhancing crop production efficiency and maintaining yield quality. Climate changeinduced challenges such as erratic weather patterns and heightened pest attacks further underscore the necessity for innovation in agriculture. At the heart of this system lies real-time sensor data, enabling precision farming practices that optimize resource utilization while fostering environmental sustainability. Leveraging Machine Learning algorithms, the system not only predicts and mitigates agricultural challenges but also detects early signs of crop diseases and nutrient deficiencies. This proactive approach ensures higher yields minimizes manual tasks and minimizes wastage. The integration of IoT technology enables remote monitoring and seamless data exchange, unlocking capabilities such as precise crop prediction, weather forecasting, and fertilizer recommendations. By analyzing historical data alongside current environmental factors, these systems accurately forecast crop yields and offer insights for informed decision-making in planting and harvesting cycles. Smart Agriculture Automation Systems embody adaptability, sustainability, and accessibility, heralding a new era of intelligent and efficient modern farming. This project integrates the Internet of Things (IoT) sensors and an innovative marketplace to revolutionize the agricultural landscape. Through this system, we aim to empower farmers with data-driven insights,

An assessment of the nutritional status of internally displaced school children in the West and Littoral Regions of Cameroon.

Malnutrition remains a major public health challenge among children in developing countries, especially those experiencing civil wars and political unrest. It is imperative to ascertain the level of malnutrition, particularly in displaced children, to develop and effectively implement interventions. This study was a cross-sectional survey conducted to assess the nutritional status of 657 internally displaced school-aged children (5-15 years) enrolled in primary schools in the West and Littoral Regions of Cameroon. The height, weight, and mid-upper arm circumference of the children were measured using standard measurements. Clinical examinations were also made on the children. Other parameters that affect nutritional status, such as morbidity and health-seeking practices, were assessed using pre-tested, structured interviewer questionnaires. An analysis of the data revealed that stunting was the most prevalent form of malnutrition (27.1%), followed by wasting (23%), thinness (21.6%), and underweight (20.1%). About 44.5% of the children had low serum iron, and 35.7% of them had poor protein status. The prevalence of anemia was 30.0%. The most common signs of protein, vitamin A, and iron deficiencies observed were thin, dry, or sparse hair (5.2%), pallor (7.3%), and xerosis (3.3%), respectively. About 32% of the children had been sick within the previous month of the study, and the most common illness was the common cold (19.8%). Most of them (>60%) had good health-seeking practices. The high prevalence of stunting, wasting, thinness, underweight, anemia, protein, and iron deficiency calls for interventions such as nutrition education and supplementation to prevent malnutrition and diet-related diseases among children.

Abstract 2198: Poverty, food insecurity, and nutritional inflammatory mediators of allostatic load

Abstract Background: Though widely recognized in cancer pathogenesis, inflammatory pathways of allostatic load remain vaguely understood. A partial explanation may be found within the realm of food insecurity and micronutrient inadequacies, defined as nutrient intake less than the estimated average requirement. Many micronutrient deficiencies contribute to pro-inflammatory and immunocompromised milieus. Inadequacies, however, which may present without overt symptoms, are not currently well studied. The purpose of this study was to examine relationships between known inflammation-associated nutritional biomarkers of micronutrients and biomarker variables of allostatic load. Methods: Using NHANES 2017/2018 surveys, adults &amp;gt; 18 years without renal or hepatic disorders, pregnancy, alcohol, or supplement use were included. Plasma levels of vitamins A, B9, C, D, E, calcium (Ca), ferritin (Fe), phosphorus (P), potassium (K) and sodium (Na) were grouped into deficiency, inadequate and adequate groups based on clinically recognized standards. Micronutrient prevalence was assessed and variables of allostatic load [blood pressure, glucose, albumin, creatinine, red cell distribution width (RDW), high density lipoprotein (HDL), low density lipoprotein (LDL), cholesterol and triglycerides] were analyzed for mean differences among micronutrient groups, food security and poverty. Results: From 3,317 unique participants, inadequacy prevalence percentages were quantified as: vitamins A (0.4%), B9 (12.2%), C (58.3%), D (39.6%), E (21.0%), P (11.4%), K (3.4%), Na (24.2%), Ca (59.5%), and Fe (37.4%) [p &amp;lt; 0.001]. Statistically significant differences were identified in age [C, D, E, Ca, Fe, P, Na (p &amp;lt; 0.004)], race/ethnicity [B9, C, D (p &amp;lt; 0.006)], gender [C, E, Ca, Fe, P, K, Na (p &amp;lt; 0.018)], poverty [A, B9, C, D, E, Fe, K (p &amp;lt; 0.025)], and food security [B9, C, D, E, P (p &amp;lt; 0.032)]. Associations to allostatic load were discovered in: albumin [A, C, D, Ca, Fe, P, Na (p &amp;lt; 0.025)], glucose [C, E, Fe, P, Na (p &amp;lt; 0.009)], HDL [C, D, E, Fe, P, K (p&amp;lt;0.045)], LDL [E, Ca, Fe, Na (p&amp;lt;0.028)], creatinine [C, D, Ca, Fe (p&amp;lt;0.001)], triglycerides [B9, D, E, Fe (p&amp;lt;0.033)], cholesterol [C, D, E, Ca, Fe, Na (p&amp;lt;0.006)], B/P [C, E, Fe, P, Na (p &amp;lt; 0.035)], and RDW [C, D, Ca, Fe (p &amp;lt; 0.001)]. Poverty was significantly associated with all variables of allostatic load (F = 2.85, p = 0.002). Food security was significantly correlated with albumin (p &amp;lt; 0.001), glucose (p = 0.005) and HDL (p &amp;lt; 0.001). Conclusion: Allostatic load, poverty, and food security were significantly associated with subclinical micronutrient statuses, which may not present with signs of deficiency and are not currently well assessed. Similarly, poverty was significantly associated with all variables of allostatic load, though food security was only identified to be associated with albumin, glucose, and HDL, suggesting that food security may not be currently adequately defined for surveillance. Citation Format: Jennifer M. Crook, Eunkyung Lee, Opeyemi Bolajoko, Rui Xie. Poverty, food insecurity, and nutritional inflammatory mediators of allostatic load [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2198.

Evaluation of anti-Fel d 1 IgY ingredient for pet food on growth performance in kittens.

The domestic cat (Felis catus) is one of the most common pets. Worldwide, approximately one in five adults are sensitive to cat allergens. The major cat allergen is the secretoglobulin Fel d 1, which is primarily produced in the salivary and sebaceous glands. Chickens produce IgY antibodies, which are similar in structure to mammalian IgG. When chickens are exposed to Fel d 1, anti-Fel d 1-specific IgY (AFD1) is produced and is naturally concentrated in egg yolk. The aim of this study was to evaluate the tolerability, effects on growth and food consumption, and potential adverse effects of a chicken egg product ingredient containing AFD1 in kittens. This was a blinded, controlled study. Twenty-seven (27) eight-week old kittens were randomly assigned to three feeding groups containing 0 ppm AFD1 (Group 0), 8 ppm AFD1 (Group 1), and 16 ppm AFD1 (Group 2) for 84 days. Veterinary exams and bloodwork were performed on Day 42 and Day 84, and body weight and body condition score (BCS) were monitored weekly. Throughout the study, there were no signs of nutritional deficiency or adverse clinical events in any of the subjects. Administration of a chicken egg product ingredient containing AFD1 in the diet (whether in coating or combination of coating and top dress) had no significant effect on body weight nor food consumption, and all subjects maintained a healthy Body Condition Score (BCS) throughout the study. Moreover, there were no biologically significant differences in the mean clinical chemistry and hematology parameters. This study demonstrated that a diet formulated to contain up to 16 ppm AFD1, included in the coating and the top-dress of dry kitten food, was well tolerated, promoted adequate growth, and exhibited no adverse effects.

N2-Acetylornithine deacetylase functions as a Cys-Gly dipeptidase in the cytosolic glutathione degradation pathway in Arabidopsis thaliana.

Glutathione (GSH) is required for various physiological processes in plants, including redox regulation and detoxification of harmful compounds. GSH also functions as a repository for assimilated sulfur and is actively catabolized in plants. In Arabidopsis, GSH is mainly degraded initially by cytosolic enzymes, γ-glutamyl cyclotransferase, and γ-glutamyl peptidase, which release cysteinylglycine (Cys-Gly). However, the subsequent enzyme responsible for catabolizing this dipeptide has not been identified to date. In the present study, we identified At4g17830 as a Cys-Gly dipeptidase, namely cysteinylglycine peptidase 1 (CGP1). CGP1 complemented the phenotype of the yeast mutant that cannot degrade Cys-Gly. The Arabidopsis cgp1 mutant had lower Cys-Gly degradation activity than the wild type and showed perturbed concentrations of thiol compounds. Recombinant CGP1 showed reasonable Cys-Gly degradation activity in vitro. Metabolomic analysis revealed that cgp1 exhibited signs of severe sulfur deficiency, such as elevated accumulation of O-acetylserine (OAS) and the decrease in sulfur-containing metabolites. Morphological changes observed in cgp1, including longer primary roots of germinating seeds, were also likely associated with sulfur starvation. Notably, At4g17830 has previously been reported to encode an N2-acetylornithine deacetylase (NAOD) that functions in the ornithine biosynthesis. The cgp1 mutant did not show a decrease in ornithine content, whereas the analysis of CGP1 structure did not rule out the possibility that CGP1 has Cys-Gly dipeptidase and NAOD activities. Therefore, we propose that CGP1 is a Cys-Gly dipeptidase that functions in the cytosolic GSH degradation pathway and may play dual roles in GSH and ornithine metabolism.

Clinical case of delayed diagnosis of celiac enteropathy

Clinical case of delayed diagnosis of celiac enteropathy

Assessment of health status, dental hygiene, and cognitive learning of primary school children: a health-camp survey reported by a clinical pharmacist and their impact on community healthcare

Background: School health surveys serve as a cost-effective and comprehensive means to screen a large number of pediatric children, facilitating primary and secondary prevention initiatives simultaneously. Methods: A prospective cohort study was conducted in a primary school in Pune to assess the physical development of children aged 5 to 17 years. Data was collected using a pre-designed questionnaire and accurate methods. Height, weight, and BMI were measured, along with clinical assessments for dyslexia, dysgraphia, and signs of vitamin deficiency. Anthropometric measurements were taken using precise instruments. Eye and tongue examinations were conducted, and hydration status was assessed. The data was analyzed using Microsoft Excel-2022. Proper consent was obtained from school authorities and parents. Results: A total of 322 participants were assessed, the age distribution revealed 49.69% males and 50.31% females. Anthropometric measurements showed 8.70% of children with a height of 90-110 cm, 34.78% with 111-130 cm, 41.61% with 131-150 cm, and 14.91% with a height ≥150 cm. In terms of weight, 44.72% weighed 15-25 kg, 29.81% weighed 26-35 kg, 16.15% weighed 36-45 kg, and 9.32% weighed ≥45 kg. BMI analysis showed 86.96% as underweight, 11.18% within the normal range, and 1.86% as severely obese. Eye conditions included 8.7% with redness, 12.4% with irritation, 24.8% with watery eyes, and 12.4% with blurred vision. Dysgraphia risk was observed in 83.23% of participants. Conclusions: Health check-up camps can result in help in the diagnosis of major morbidities in school children and enable better practice among government schools.

The implementation of the SPAD-502 Chlorophyll meter for the quantification of nitrogen content in Arabica coffee leaves

The utilization of a non-destructive SPAD-502 chlorophyll meter, which enables the measurement of nitrogen status in plant leaves, has gained popularity in agronomic crops. Its application to horticultural crops like coffee remains relatively uncommon. The device provides quick and real-time measurements, helping to provide on-time nitrogen fertilizer to coffee plants before deficiency signs occur. Coffee leaves are characterized by thick and waxy leaves, together with many layers of tree crown. Therefore, the objective of this study was to develop a method for measuring nitrogen levels in coffee plants using the SPAD-502 Chlorophyll meter for an appropriate nitrogen fertilizer application rate in Arabica coffee plants.•Coffee trees were separated into upper, middle and lower levels. Data on SPAD values and total nitrogen were analyzed.•Pearson Correlation Coefficient (R), Coefficient of Determination (R2) and linear regression were calculated for different three levels of both SPAD-502 and total nitrogen values.•The results revealed a strong correlation (R2 = 0.63) between the SPAD readings of coffee leaves obtained from the upper canopy and their nitrogen content.These findings can provide a good concept of which coffee crown level will be a better part for measuring N content using a SPAD-502 Chlorophyll meter.

Skin and mucosal lesions in the course of HIV infection

Introduction:&#x0D; Dermatological conditions are prevalent expressions of HIV and AIDS, often aiding in initial diagnoses due to their visibility on the skin and mucous membranes. Given HIV's primary transmission through sexual contact, dermatologists play a crucial role in identifying and managing these infections. Understanding the range of skin and mucosal lesions occurring throughout the course of the disease is vital, particularly as these symptoms can manifest in atypical ways or even precede other signs of immune deficiency. While most associated skin disorders can be treated in outpatient settings, some severe conditions significantly impact health outcomes.&#x0D; Aim of the study:&#x0D; The study aims to consolidate knowledge on the typical manifestations of HIV on the skin and mucous membranes, providing a comprehensive overview of these disease entities. The most common skin and mucosal symptoms associated with HIV infection are summarized and described.&#x0D; Materials and methods:&#x0D; The literature available in the PubMed database was reviewed using the following keywords: “HIV”, “AIDS”, “Skin lesions”, “Mucosal lesions”.&#x0D; Conclusions:&#x0D; Skin conditions are widespread in HIV-infected individuals, often displaying heightened severity compared to the general population. While many of these dermatological issues aren't unique to this group, their manifestations can be more pronounced. HIV-related skin problems vary widely, including specific eruptions linked to AIDS, opportunistic infections, and AIDS-related cancers. Although some HIV-associated skin diseases have decreased due to antiretroviral therapy, there's been a noticeable increase in drug reactions and other non-infectious skin conditions.

COMPARING NUTRIENT MANAGEMENT STRATEGIES IN DECOUPLEDAQUAPONICS SYSTEMS FOR GROWING Cannabis sativa

Aquaponics is an integrative aquaculture system that uses waste produced by farmed aquatic animals to supply nutrients to a hydroponic system for growing plants. The growth of Cannabis sativa in aquaponic systems displays the potential for well-managed aquaponic systems to produce a variety of crops. In this study, we examined the impact of nutrient management techniques on the growth of Cannabis sativa in a decoupled aquaponic system. Four treatments were assessed: (1) recirculating treatment using aquaponic system effluent plus nutrient supplementation (AqRN), (2) flow-through treatment with aquaponic system effluent (AqFT), (3) flow-through hydroponic treatment with aquaponic effluent supplementation (10% by volume) (AqHydro), and (4) flow-through hydroponic control treatment (Hydro). The results indicated that the AqFT and AqHydro systems provided comparable yields to the hydroponic system, while the AqRN treatment exhibited reduced trimmed yield with signs of plant stress and nutrient deficiency appearing toward the end of the trial. AqFT produced fewer terpenes than the other treatments and may benefit from micronutrient supplementation. AqHydro and Hydro treatments displayed no significant difference in water quality or yield. Notably, AqHydro treatment showed reduced water consumption, underscoring the potential for aquaculture effluent supplementation to cost-effectively enhance fertigation in hydroponic systems.

46,XX males with congenital adrenal hyperplasia: a clinical and biochemical description.

Congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21OHD) or 11-hydroxylase deficiency (11OHD) is characterized by underproduction of cortisol and overproduction of adrenal androgens. These androgens lead to a variable degree of virilization of the female external genitalia in 46,XX individuals. Especially in developing countries, diagnosis is often delayed and 46,XX patients might be assigned as males. This study aims to describe the clinical and biochemical characteristics of a unique cohort of untreated male-reared 46,XX classic CAH patients from Indonesia and discusses treatment challenges. Nine untreated classic CAH patients with 46,XX genotype and 21OHD (n=6) or 11OHD (n=3), aged 3-46 years old, were included. Biometrical parameters, clinical characteristics, and biochemical measurements including glucocorticoids, renin, androgens, and the pituitary-gonadal axis were evaluated. All patients had low early morning serum cortisol concentrations (median 89 nmol/L) without significant increase after ACTH stimulation. Three patients with salt wasting 21OHD reported one or more periods with seizures and/or vomiting in their past until the age of 6, but not thereafter. The remaining patients reported no severe illness or hospitalization episodes, despite their decreased capacity to produce cortisol. In the 21OHD patients, plasma renin levels were elevated compared to the reference range, and in 11OHD patients renin levels were in the low-normal range. All adult patients had serum testosterone concentrations within the normal male reference range. In 21OHD patients, serum 11-oxygenated androgens comprised 41-60% of the total serum androgen concentrations. Glucocorticoid treatment was offered to all patients, but they refused after counseling as this would reduce their endogenous androgen production and they did not report complaints of their low cortisol levels. We describe a unique cohort of untreated classic 46,XX male CAH patients without overt clinical signs of cortisol deficiency despite their cortisol underproduction and incapacity to increase cortisol levels after ACTH stimulation. The described adolescent and adult patients produce androgen levels within or above the normal male reference range. Glucocorticoid treatment will lower these adrenal androgen concentrations. Therefore, in 46,XX CAH patients reared as males an individual treatment approach with careful counseling and clear instructions is needed.

A deep learning-based algorithm for crop Disease identification positioning using computer vision

Food security is fundamental to a country. As the main risk factors, pests and diseases seriously restrict the normal growth of crops and the quality and safety of agricultural products. With the intensification of climate change and the continuous adjustment of farming methods, crop diseases and pests have become more frequent in recent years. Therefore, the agricultural production mode has gradually moved from family production to large-scale agricultural planting, and the production equipment has become more automated and intelligent. Agricultural intelligent robots can reduce labor costs in the process of agricultural production and improve the standardization of agricultural production. The application of computer vision in agriculture is rapidly becoming an important aspect of modern agricultural technology, especially in crop positioning and management. Through the use of advanced image processing algorithms and pattern recognition technology, computer vision systems are able to accurately identify and locate various crops in the field, enabling automated and precise management. This technology shows great potential for crop health monitoring, pest identification, and maturity assessment. For example, by analyzing images of plants, computer vision systems can spot signs of lesions or nutrient deficiencies in time and guide farmers to treat them accordingly. In addition, this technology can also be used to guide automated agricultural machinery, such as driverless tractors and harvesters, to improve the efficiency of crop harvesting and reduce labor costs. In general, the combination of computer vision and crops provides new technical means for the development of modern precision agriculture, which helps to improve the efficiency and sustainability of agricultural production.

EFFECT OF HUMIC FEED ADDITIVE ON METABOLIC PROCESSES AND PRODUCTIVITY OF BEEF CATTLE

The use of made in Kazakhstan organic and mineral feed additives to in-crease the meat productivity of cattle is important for the agro-industrial complex of the Republic. During the study, qualitative and quantitative analysis of raw materials was made. Quality control of the finished feed additive was carried out following the requirements of the State Pharmacopoeia of the Republic of Kazakhstan. The studies were conducted in accordance with the requirements of the Technical Regulations approved by the Decree of the Government of the Republic of Kazakhstan dated April 23, 2008 No. 380, Regulation No. 7-1/625 dated 11/28/2014 and Regulation (EC) No. 1831/2003 of the European Parliament and of the Council of September 22, 2003, on additives for use in animal feeding. Kazakh potassium humate of the Maykubensky deposit is sterile, non-pyrogenic, non-toxic, and harmless, with an average pH of 11.0-1.0, and has a biological effect on laboratory animals (white mice). The effectiveness analysis in the production experiment allowed us to identify the positive effect of feed additives on metabolic processes and increasing meat productivity in bulls. Regardless of age, on the 7th day of taking the feed additive of potassium humate, the calves' coats acquired a noticeable shine and a bright color, and shedding was completed with-in a month. Clinical signs of metabolic disorders and vitamin deficiency, detected in the farm at the time of the experiment in some animals, pass through 20-30 days from the beginning of the application of the feed additive. The optimal scheme for the use of feed additives was tested on the Kazakh meat breed Akbas, and the concentration of humic substances was selected for the maximum mani-festation of the biologically active effect. Animals became mobile and active, their rumination became more active, they grew noticeably, and the average daily weight gain increased by 7.6-8.9%.

Folate - a scoping review for Nordic Nutrition Recommendations 2023.

Folate is an essential micronutrient for normal development and metabolic function, and folate deficiency is associated with an increased risk of cancer, cardiovascular disease, mental dysfuntion and negative pregnancy outcomes. When estimating folate requirements, one must consider different bioavailability and functionality between synthetic folic acid and dietary folate, together with increased needs of folate in women of fertile age, pregnant and lactating women, preterm and small for gestational age weight infants and individuals who are homozygote for the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism. In order to achieve an adequate metabolic status based on the metabolic marker total homocysteine, and not merely the absence of clinical signs of folate deficiency, the recommended intake of folate differs according to age, pregnancy and lactation. According to the World Health Organization, a decision limit for folate deficiency in adults is serum folate level below 10 nmol/L, and in women of fertile age a red blood cell folate level below 906 nmol/L in order to prevent neural tube defects. Qualified systematic reviews along with identified relevant literature have been used for this scoping review prepared for the Nordic Nutrition Recommendations 2023.

Assessing nutrition status, sarcopenia, and frailty in adult transplant recipients.

The assessment of nutrition status, sarcopenia, and frailty holds significant relevance in the context of adult transplantation, as these factors are associated with an unfavorable prognosis; thus, transplant candidates must undergo a full nutrition assessment. Screening tools may be used to prioritize patients, this can be done using the Nutrition Risk Screening 2002 or Royal Free Hospital-Nutritional Prioritizing Tool. Subsequently, a thorough nutrition-focused physical examination should be conducted to evaluate clinical signs of nutrition deficiencies, fat and muscle loss, and fluid overload; dietary history and current intake must also be assessed. Apart from physical examination, specific testing for sarcopenia and frailty are recommended. For sarcopenia assessment, specifically for muscle quantification, the gold standard is the cross-sectional measurement of the muscle at L3 obtained from a computed tomography scan or magnetic resonance imaging; dual-energy x-ray absorptiometry is also a good tool especially when appendicular skeletal muscle index is calculated. Other more readily available options include phase angle from bioelectrical impedance or bioimpedance spectroscopy. In the sarcopenia assessment, muscle function evaluation is required, handgrip strength stands as the primary test for this purpose; this test is also part of the subjective global assessment and is included in some frailty scores. Finally, for frailty assessment, the Short Physical Performance Battery is useful for evaluating physical frailty, and for a multidimensional evaluation, the Fried frailty phenotype can be used. Specifically for liver transplant candidates, the use of Liver Frailty Index is recommended.

Molybdenum - a scoping review for Nordic Nutrition Recommendations 2023.

Molybdenum is an essential element in the form of the molybdenum cofactor (Moco). In humans, Moco is required for four enzymes: xanthine oxidase (XO), aldehyde oxidase, sulfite oxidase (SO), and mitochondrial amidoxime-reducing component (mARC). The enzymes are involved in the oxidation of purines to uric acid, metabolism of aromatic aldehydes and heterocyclic compounds, and in the catabolism of sulfur amino acids. Molybdenum cofactor deficiency is a rare autosomal recessive syndrome due to a defective synthesis of Moco, resulting in a deficiency of all the molybdoenzymes. There are no reports on clinical signs of dietary molybdenum deficiency in otherwise healthy humans. Water-soluble molybdate is efficiently absorbed from the digestive tract. The body retention is regulated by urinary excretion. Plasma molybdenum reflects long-term intake and 24-h urinary excretion is related to recent intake. There are no biochemical markers of molybdenum status. Cereal products are the main contributors to molybdenum dietary intake, estimated to 100-170 μg/day in Nordic studies. Little data are available on molybdenum toxicity in humans. A tolerable upper intake level of molybdenum has been based on reproductive toxicity in rats, but the effects have not been reproduced in more recent studies. The U.S. Institute of Medicine (IOM, present National Academy of Sciences, Engineering, and Medicine; NASEM) established a Recommended Dietary Allowance of 45 μg/day in adult men and women in 2001, based on a small study reporting urinary excretion in balance with intake at 22 μg/day. The European Food Safety Authority (EFSA) considered in 2013 the evidence to be insufficient to derive an Average Requirement and a Population Reference Intake, but proposed an Adequate Intake of 65 μg/day for adults.

Assessment of risk factors for osteopenia development in premature babies

Neonatal osteopenia (KB 61.3 (ICD-11)) – violation of mineral exchange of bone tissue (BT) of premature babies (PB). Predicting and detection of osteopenia is the key to its successful control.&#x0D; Aim. The aim of the article was to determine risk factors influencing the formation of neonatal osteopenia of PB and during the term of intensive care / postintensive nursing.&#x0D; Materials and methods. Ultrasound densitometer Sunlight Omnisense 9000 was used to measure the ultrasound speed in BT (SOS, m/s) with estimation Z-score (SD) according to gestational age of 56 PB and 20 mature babies (control group) in the early neonatal age. Verification of osteopenia was carried out under the WHO guidelines for radiation methods by Z-score indicator less than SD by -1.0. Estimation of Z-score -2.0 SD and less was attributed to ultra-low indicators. The characteristics of obstetric and gynecological, somatic anamnesis and pregnancy course of premature babies’ mothers, factors of PB postnatal development that influence the formation of BT were studied.&#x0D; Results. Children born at 33 weeks or earlier are 3.23 times more likely (OR = 3.23; CI 95 % [1.08; 9.70]) to develop BT demineralization by the corresponding term of birth than other PB. The chances of ultra-low SOS by Z-score are 14.22 times higher (OR = 14.22; CI 95 % [3.29; 61.57]) in PB born at 32 weeks of gestation and earlier. Extragenital diseases, clinical signs of calcium deficiency in mothers, and women’s intake of calcium and vitamin D3 during pregnancy did not have a statistically significant difference (p &gt; 0.05) in the studied PB and control group. The mother’s preeclampsia increases the chances of insufficient BT mineralization by 5.47 times (OR = 5.47; CI 95 % [1.07; 27.93]), second parity pregnancy (and subsequent ones) – by 4.51 times (OR = 4.51, CI 95 % [1.38; 14.80]). The factors of the ratio of the duration of mechanical ventilation relative to the total time of PB treatment, the duration of parenteral feeding have an inverse correlation (moderate (r = -0.42, р &lt; 0.05) and significant (r = -0.51, р &lt; 0.05) respectively) with a decrease in SOS to low Z-score.&#x0D; Conclusions. In PB born at 32 weeks of gestation and earlier, the chances of ultra-low SOS indicators by Z-score are 14.22 times higher (OR = 14.22; CI 95 % [3.29; 61.57]). The following factors predicts insufficient mineralization of BT according to SOS indicators according to the Z-score: mother’s second parity pregnancy (and subsequent ones), preeclampsia, parenteral feeding of PB for more than 8 days (Se = 75.00 %, Sp = 71.87 %, p &lt; 0.0001), duration of mechanical ventilation, which is more than 6.48 % of the total time of PB treatment (Se = 86.67 %, Sp = 59.38 %, p &lt; 0.046).

Features of the immune status and comorbidities in children with alopecia areata

Increased incidence of alopecia has been noted in children at the present time. Participation of autoimmune (immunopathological) mechanisms in pathogenesis of this disease necessitates additional study of immune status and characteristics of comorbid pathologies. The aim of our study was to specify the features of immune status and comorbidities in children with alopecia areata. The observation group consisted of children with various types of alopecia areata (n = 57), a comparison group included children without clinical manifestations of alopecia or a history of alopecia (n = 157). We performed a comparative evaluation of major lymphocyte subpopulations (CD3+, CD3+CD4+, CD3+CD8+, CD3+CD19+), interleukins (IL-4, IL-6), immunoglobulins (IgA, IgM, IgG), parameters of phagocytic activity (absolute phagocytosis, percentage of phagocytosis, phagocytic number and phagocytic index) and IgE to house dust and cat hair. Analysis of comorbidities was also performed. Statistical processing was carried out with Jamovi software. We have found that the focal clinical form of alopecia prevailed over the subtotal and total forms by 1.8 times (p = 0.033) in the observation group rather than in comparison group, with common variable immunodeficiency being more often (1.4-fold), chronic tonsillitis (3.9-fold), allergic rhinitis (3.9-fold) and autoimmune thyroiditis, which was absent in the comparison group. Among the children with alopecia, disturbances of the T-cell link were revealed, i.e., a higher median levels of the relative and absolute numbers of CD3+CD4+ cells (p = 0.001 to 0.003), larger proportions of elevated values for IgA (4.1-fold), IgM (7.3-fold), IgG (13.2-fold) with p-levels of 0.0001 to 0.0008; increased IL-4 (8.1-fold) and IL-6 (4.6-fold), with p = 0.002-0.004, along with medium and relatively strong correlations with alopecia. In children with alopecia, we have determined a 3.3-fold proportion of reduced values of absolute phagocytosis and 3.7-fold reduced percentage of phagocytosis (p = 0.0012 to 0.028), with an sufficient correlation (weak to moderate strength) with alopecia, as well as lower values of the median phagocytic index and phagocytic number in the observation group (p &lt; 0.001) associated with a average-strength correlation. Hence, an imbalance of the immune system components was revealed in children with alopecia which manifested with signs of immune hyperfunction, characteristic, e.g., of autoimmune and allergic processes, accompanied by more frequent registration of autoimmune thyroiditis and allergic rhinitis. Moreover, distinct signs of immune deficiency, are found, characterized by a decrease in phagocytic activity and higher incidence of common variable immunodeficiency and chronic tonsillitis.