Objective To explore the application of body position intervention combined pronase in gastric mucosal cleaning in painless gastroscopy. Methods A total of 200 patients who underwent painless gastroscopy from July 2016 to July 2017 in the digestive endoscopy center were selected as the subjects. According to the random digital table method, the patients were randomly divided into the experimental group and the control group of 100 cases. In the experimental group, before the gastroscope was examined, pronase plus Dimethicone Powder and lidocaine mucilage was used, and then the body position intervention (right supine 5 min-supine 5 min-left lying position 5 min) was examined, and the control group was taken Dimethicone Powder and Lido before the intensive examination. The caking mortar was then placed on the left side of the examination bed 15 min for examination. The upper gastrointestinal tract visual field definition and endoscopic operation time were compared between the two groups under magnifying endoscopy under white light and narrowband imaging. Results In the experimental group, 72.0% (72/100), 20.0% (20/100), 6.0% (6/100) and 2.0% (2/100) of A, B, C, D grade of the visual field clarity of mucosa under white light were better than 32.0% (32/100), 30.0% (30/100), 13.0% (13/100) and 25.0% (25/100) of the control group, respectively. The difference was statistically significant (χ2= 39.54, P < 0.05). There were 0, 6, 29 and 65 cases of 1, 2, 3, 4 scores of microvascular visual field intelligibility scores under magnifying endoscopy combined with narrow band imaging in the experimental group, which were better than those in the control group (11, 31, 28 and 30 cases respectively). The difference between the two groups was statistically significant (Z=-6.07, P < 0.05). The examination time of the experimental group was (10.64 ± 3.83) minutes, which was lower than that of the control group (11.67 ± 4.89) minutes, and the difference was statistically significant (t=1.978, P < 0.05). Conclusions The effect of pronase as an anti mucilage agent combined with body position is obvious, and the effect of dispelling the mucus and removing the mucus is comprehensive, and it can effectively shorten the time of examination. It is worthy of clinical application. Key words: Posture intervention; Painless gastroscopy; Pronase; Visual clarity