Showed Inhibition Activity Research Articles

Synthesis, crystal structure, molecular docking studies and biological evaluation of aryl substituted dihydroisoquinoline imines as a potent angiotensin converting enzyme inhibitor

The different functionalities of imine group found in many compounds are of important biological activity. Since, the development of novel imines is biologically encouraged, this study aims to develop a straightforward synthesis pathway of dihydroisoquinoline imines that could reveal antihypertensive effects. Starting from 2-méthyl-1-phényl-2-propanol, four different dihydroisoquinoline imines compounds (2a-d) were obtained and their structures were analyzed by mass spectrum, elemental analysis and NMR spectral studies. The crystal structure of 2b was determined from single crystal X-ray diffraction data.The newly synthesized compounds were evaluated for angiotensin I-converting enzyme (ACE) inhibition, using an enzymatic in vitro assay. The results were compared to Captopril as a reference drug. Compounds 2a, 2b and 2d showed inhibition activity with IC50 values of 0.15, 0.13 and 0.40 mg/ml, respectively. The docking of chemical compounds in the ACE active site explained the higher inhibitory capability of compounds 2a and 2b on the catalytic activity of the enzyme, indicating a potential anti-hypertensive effect of these compounds.

Antimicrobial activities of crude solvent extract and synthesized silver nanoparticles of Opuntia ficus-indica L. Cladodes

Opuntia ficus-indica cladodes was examined for their antimicrobial activities by using crude extract and synthesized silver nanoparticles against human pathogenic microbial strains, namely Staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis and Candida albicans. All extracts demonstrated moderate activity against the tested microbes in a range between 0.53 and 2.33 cm. The maximum inhibition activity was found to be against K. pneumoniae while the lowest against S. aureus. Synthesized silver nanoparticles (Ag-NPs) showed inhibition activities between 1.82 and 2.03 cm. The maximum activity was recorded against K. oxytoca, while the lowest activities against P. mirabilis. In context of antimicrobial activity, there was no significant difference either solvent crude extract or synthesized silver nanoparticles against tested microbes. Therefore, crude extracts of O. ficus-indica cladodes or Ag-NPs could be used as an alternative natural drug.

Screening antibiofilm and quorum-quenching activity of Actinomycetes isolates against aquatic pathogens

Background: Indonesia aquatic industry continually experience the same challenges, namely bacterial outbreaks which result in economic losses and could also endanger food security of Indonesia. This condition is exacerbated by the increasing prevalence of resistance of pathogenic bacteria to various antibiotics. The formation of biofilms structure is one important aspect in the emergence of the nature of resistance in several bacteria. In this case, the formation of this structure is regulated by communication system owned by bacteria, namely the quorum sensing system. Therefore, many researchers are now using this approach through this system to both inhibit and destroy the structure of biofilms, hoping to reduce the prevalence of bacterial resistance to various antibiotics. In this study, we attempted to screen and characterize bioactive compounds isolated from isolates of Actinomycetes which perform antibiofilm and quorum quenching (anti-quorum sensing) activity against aquatic pathogen. Methods and materials: Actinomycetes isolates were grown in yeast malt extract agar +1% calcium carbonate as their differential media. In this study, screening of bioactive compound of Actinomycetes isolates were performed by using liquid-liquid extraction. In addition in this study, we also performed antibiofilm activity assay in two different categories based on its mechanisms (inhibition and destruction activity) against biofilm structure forming by Vibrio harveyi, Aeromonosa hydrophila, and Streptococcus agalactiae ATCC 27956. We also characterized bioactive compounds of these isolates which showed quorum quenching activity. Results: We found sixteen isolates of Actinomycetes which showed quorum quenching activity, based on the characterization of quorum quenching activity we found that there were several isolates, such as 16PM and 18PM which showed protein as its major quorum quenching activity. In addition, these isolates were screened for their antibiofilm activity against biofilm structure forming by Vibrio harveyi, Aeromonas hydrophila, and Streptococcus agalactiae. We found that SW03 isolates showed inhibition activity against biofilm structure of V. harveyi for up to 77%, whereas 12AC isolates showed destruction activity against biofilm structure of V. harveyi for up to 59%. Conclusion: This study revealed antibiofilm and quorum quenching activity Actinomycetes isolates potentially to be applied in the prevention and treatment of infectious disease in aquatic environment.

Preliminary Report on the Effect of Savanna Plants Leucaena leucocephala, Parkia platycephala and Senna alata against Eggs and Immature Stages of Trichostrongylid Nematodes In Vitro.

The current study evaluated the anthelmintic effect of different extracts of Leucaena leucocephala, Parkia platycephala, and Senna alata on trichostrongylid eggs and infective larvae and determined the potential active components of each plant. Dried and macerated plant material was concentrated using rotaevaporation to obtain the crude extract (CE), followed by solvent partitioning to obtain hexanic (HexE), acetatic (AcE), and butanolic (BuE) extracts used for phytochemical analysis and anthelmintic efficacy testing in vitro. All the crude and partitioned extracts tested showed inhibition activity in the hatching of trichostrongylid eggs. Larvicidal efficacy was observed at CE concentrations of 2.5, 5.0, and 7.5 mg/mL for P. platycephala and S. alata. However, L. leucocephala CE did not significantly reduce the number of living larvae in the tested concentrations. Chromatographic analysis revealed several active metabolites; gallic acid, ellagic acid, naringin, morin, and kaempferol on AcE of P. platycephala; gallic acid, rutin, and ellagic acid on BuE of P. platycephala; and gallic acid and naringin on BuE of L. leucocephala. The extracts of P. platycephala, L. leucocephala, and S. alata leaves showed egg hatching inhibition and larvicidal activity, probably produced by tannins and flavonoids, which may act alone or by synergism.

N-methoxy-β-carboline alkaloids with inhibitory activities against Aβ42 aggregation and acetylcholinesterase from the stems of Picrasma quassioides

Nine new N-methoxy-β-carboline alkaloids (NMCAs) (1a/1b–3a/3b and 4–6) and two known NMCAs (7 and 8) were isolated from the stems of Picrasma quassioides. Their structures were elucidated by spectroscopic data analyses, quantum chemical calculations, and single-crystal X-ray crystallographic data. An analysis of the 13C NMR chemical shifts of the N-methoxy groups in these NMCAs and 41 gathered known compounds reveals the phenomenon that the chemical shifts of all these N-methoxy groups are greater than δC 62, which can be used to recognize the N-methoxy group rapidly. In addition, the acetylcholinesterase (AChE) and Aβ42 aggregation inhibitory activities of 1–8 were evaluated. Compounds 1, 2, 7, and 8 displayed AChE inhibitory activity with IC50 values of 14.9, 13.2, 17.6, and 43.9 μM, respectively. Compound 2 showed inhibition activity against Aβ42 aggregation with an IC50 value of 10.1 μM.

Physico-chemical characterization, metabolomic profile and in vitro antioxidant, antihypertensive, antiobesity and antidiabetic properties of Andean elderberry (Sambucus nigra subsp. peruviana)

BACKGROUND: The consumption of fruits, mainly berries, has been associated with beneficial health properties. These properties would be related to the quantity and quality of the metabolites present. Some native berries from the Andes, such as Andean elderberry, would be an important source of metabolites with bioactive properties. OBJECTIVES: To evaluate at the physico-chemical and metabolomic level the characteristics of the elderberry from four different high Andean regions, and to determine their bioactive potential through in vitro tests. RESULTS: Significant differences were found in the evaluated characteristics according to the Region of procedence of the fruit. Glucose was the main sugar for all berry samples, followed by fructose. Among the most important organic acids, citric and malic acid were found. Elderberry from Apurímac presented the highest content of total sugars and lower content of total acids in comparison to the samples from other procedences. UPLC-PDA results indicate that Junín elderberries presented the highest value of total phenolic compounds with high contents of flavonols, hydroxycinnamic acids and anthocyanins. Andean elderberry presented cyanidin 3-O-glucoside and cyanidin 3-O-sambubioside as main anthocyanins as well as their respective isomers. All samples showed inhibition activities against the different evaluated enzymes. Elderberries from Apurímac and Junín, displayed the lowest values of IC50 for the inhibition of α-amylase and α-glucosidase, while for the inhibition of lipase and angiotensin converting enzyme (ACE-I) elderberries from Junín displayed the lowest IC50 values. CONCLUSIONS: The results of this study suggest that Andean elderberry could be considered as an important source of bioactive compounds with properties that could be highlighted by the nutraceutical and functional food industries.

Identification of HDAC6 selective inhibitors: pharmacophore based virtual screening, molecular docking and molecular dynamics simulation

HDAC6 regulates the expression and activity of various tumor-related proteins, but currently there is no selective inhibitor targeting HDAC6 for clinical application. In order to discover novel HDAC6 inhibitors, virtual screening methods comprised of pharmacophore based virtual screening, molecular docking and molecular dynamics (MD) simulations were employed. 15 molecules were obtained after virtual screening. After in vitro bioassays, two of the hits showed inhibition activity against HDAC6, among which the inhibition activity of G1 to HDAC6 reached 81% at concentration of 20 μM. In addition, the inhibitory activity against HDAC1 and HDAC10 demonstrated that G1 and G10 were highly selective to HDAC6. The analysis of the binding modes of G1 and G10 provides a reference for further development of highly active HDAC6 inhibitors. Communicated by Ramaswamy H. Sarma

IMPACT OF ENDOPHYTIC LIPOPEPTIDES USE AGAINST SOME PLANT PATHOGENIC MICROORGANISMS

Twelve dissimilar plant associated bacterial colonies were isolated from five different tomato seedlings, the Non Ribosomal Lipopeptides genes were detected by degenerated primers technique in the twelve tomato endophytic isolates, only the long rode bacilli endophytic strains were found to harbour the lipopeptides synthetases genes, these three strains were given laboratory names of (BMG100, BMG101 and BMG102) for further pursuit. Partial sequencing of 16s RNA gene and protein profile analysis for the three bacilli isolates showed that all isolates belonged to Bacillus subtilis. The lipopeptides production by HPLC analysis revealed that all the endophytic isolates from tomato stems had the ability to produce lipopeptides except the third isolate named B. subtilis BMG102 showed no production. different levels of volumetric oxygen transfer coefficient (KLa) were used as cursors for lipopeptides production. The maximum concentration of surfactin type was about 1431, 529 and 437 mg.L-1, the plipastitin (fengycin type ) was 360 198 mg.L-1 and zero while the Mycosubtilin (iturin type) was zero, 228 and 414 mg.L-1 for the strains B. subtilis ATCC21332, BMG100 and BMG101, respectively. Surfactin production is visibly optimal at high oxygen transfer (kLa) and showed more than 0.07 S-1, while the optimum production of the fengycin and iturin could be observed at mild values of O2 transfer (kLa) which equal 0.01 and 0.015 S-1 respectively. The antibacterial - antifungal effects of the three B.subtilis extracts collected during lipopeptides productivity regimes showed inhibition activity against Pseudomonas syringae, Ralstonia solanacearum and Erwinia amylovora, as will as the antifungal activity against Aspergillus niger, Phanerochaete chrysosporium and Alternaria alternata. These results indicate potential use of B.subtilis strains as biocontrol agents, especially when considering commercial implementation. Studies regarding the lipopeptides applications, should also be considered as this will influence the efficacy of the lipopeptides against the target organisms.

Reinforced ZnONPs/ rosemary essential oil-incorporated zein electrospun nanofibers by κ-carrageenan

In this study, the reinforced ZnONPs/ rosemary essential oil-incorporated zein nanofibers with κ-carrageenan (Z/KC/ZnONPs/RE) were fabricated using the electrospinning technique for application in food packaging. The SEM images of Z/KC/ZnONPs/RE nanofiber displayed bead-free homogeneous morphology that its average fiber diameter was 672 ± 240 nm. The formation of new hydrogen bonds by addition of κ-carrageenan and active agents was approved by FT-IR and DSC structural conformations. The Z/KC/ZnONPs/RE nanofiber exhibited satisfactory thermal and mechanical properties, as well as high surface hydrophobicity. In addition, the Z/KC/ZnONPs/RE sample showed inhibition activity against S. aureus (18.5 ± 1.9 mm) and E. coli (14.7 ± 1.5 mm) bacteria. The DPPH scavenging activity of Z/KC/ZnONPs/RE nanofiber was 54.5 ± 3.6 %. Additionally, the fabricated nanofibers showed no cell cytotoxicity, indicating their good biocompatibility. Thus, we believe that the fabricated Z/KC/ZnONPs/RE electrospun nanofiber is a potential candidate for using as an active layer in food packaging system.

Identification and partial purification of thuricin 4AJ1 produced by Bacillus thuringiensis.

Thuricin 4AJ1, produced by Bacillus thuringiensis strain 4AJ1, showed inhibition activity against Bacillus cereus 0938 and ATCC 10987. It began to appear in the stationary phase and reached its maximum activity level of 209.958 U at 18h against B. cereus 0938 and 285.689 U at 24h against B. cereus ATCC 10987. Tricine-SDS-PAGE results showed that the partly purified thuricin 4AJ1 was about 6.5kDa. The molecular weights of the known B. thuringiensis bacteriocins and the ones obtained by the two mainstream websites for predicting bacteriocins were inconsistent with the size of the thuricin 4AJ1, indicating that the bacteriocin obtained in this study may have a novel structure. Based on the biochemical properties, the thuricin 4AJ1 activities increased after treatment with proteinase K and lipase II, and were not affected by a-amylase, catalase, α-chymotrypsin VII and α-chymotrypsin II. It was heat tolerant, being active up to 90ºC. In the pH 3-10 range, it maintained most of its activity. Finally, the sensitivity of the strain 4AJ1 to commonly used antibiotics was tested. In view of its stability and antibacterial activity, thuricin 4AJ1 may be applied as a food biopreservative.

Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado

The emergence of drug resistant and multidrug-resistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better anti-infective drug.. This study was aim to screen potential antiinfective extracts of sponges collected from Bunaken, Manado and to report on their antibacterial and antifungal properties. Testing for antiinfective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coli and Salmonella typhi were used as the testing bacteria and Candida albicans as the testing fungi. Chloramphenicol was used as positive control for anti bacterial activity and Ketokonazol for antifungi. The two acetone extracts tested (MD-01aceton and MD-02aceton), all of them showed inhibition activities. Following partition with chloroform and methanol, all chloroform extracts inhibited the growth of both bacteria and fungi. These extracts then are considered to be potential candidates for further isolation and characterization as antiinfective agents.

Antibacterial activity of different crude extracts of Suaeda maritima used traditionally for the treatment of hepatitis

Traditionally, plants and their products are used as a folk medicine for the treatment of curable and incurable diseases for a long time in many areas of the world. However, there is a lack of systematic study of the antibacterial activity of Suaeda maritima (S. maritima). The present study is to estimate the antibacterial activity of various extracts of newly discovered species S. maritima plant which is collected from Oman. The methanol extract was prepared by the Soxhlet method and it was fractionation by different solvents to give different crude extracts. The antibacterial activity was assessed by using the agar disc diffusion method in which the extracts at different concentrations were applied to the disc by putting them in sterile filter paper of about 6 mm. The agar discs were incubated for 24 h and measure the inhibition zone of antibacterial activity. The range of the inhibition zone was between 7 and 12 mm. Overall all concentrations of each extract of S. maritima showed inhibition activity against the applied bacterial strains. The results found that the hexane extract at all concentrations was possessed the highest activity against the used two Gram-positive and two Gram-negative bacteria strains. In conclusion, the highest antibacterial activity hexane extract from the S. maritima could be used for the treatment of infectious diseases. In addition, the observed antibacterial activity of the selected plant could be due to the presence of high contents various group bioactive compounds.

In vitro anti-diabetic activity of Tribulus terrestrisL. fruits extracts

PurposeThe purpose of this paper is to evaluate the inhibition activity of Tribulus terrestris L. (T. terrestris) fruits extracts with solvents of increasing polarity against α-glucosidase and α-amylase, and to determine the inhibition mode of the most effective extract against both enzymes.Design/methodology/approachHexane, acetone, ethanol and aqueous extracts of T. terrestris fruits were prepared using ultrasonic sequential extraction and analyzed for their α-amylase and α-glucosidase inhibitory activities by specific assay for each enzyme. The modes of inhibitions were detected using Lineweaver–Burk plots.FindingsT. terrestris fruits extracts showed inhibition activity against α-glucosidase and α-amylase which was in the dose-dependent manner. Hexane extract had the highest α-glucosidase inhibition activity (IC50 = 27.28 μg/ml, p = 0.003), followed by acetone and ethanol extracts (IC50 = 60.58 μg/ml and IC50 = 84.21 μg/ml, respectively). The inhibition mode of hexane extract was noncompetitive. While acetone extract showed the highest inhibition activity against α-amylase (IC50 = 6.18 mg/ml, p = 0.002), hexane and ethanol extracts showed no significant difference (IC50 = 13.04 mg/ml and IC50 = 14.20 mg/ml, respectively, p = 0.09). The inhibition mode of acetone extract was competitive.Originality/valueT. terrestris fruits extracts had strong inhibition activity against α-glucosidase and α-amylase, and they can be used as a promising anti-diabetic agent.

Occurrence of Enterocin Genes in Enterococci from Slovak Milk Product Žinčica

Abstract Žinčica is a popular Slovak dairy product made from ewes’ milk. It is a by-product resulting during ewes’ lump cheese processing. Microbiota in Žinčica have rarely been studied, especially enterococci; however, they can produce beneficial substances – bacteriocins. In this study, the presence of Enterocins (Ents) genes were analysed in enterococci from Žinčica and partially also the inhibition activity. Samples of Žinčica were collected from different agrofarms producing ewes’ lump cheese (34) in Central Slovakia. In the enterococci tested, Ent P gene was the most frequently detected (in 6 out of 7 enterococci), followed by Ent A and Ent L50B genes. Ent B gene was detected only in E. faecium 30E1. On the other hand, E. faecalis 31E2 did not contain Ent genes, although it showed inhibition activity against the indicator strains Enterococcus avium EA5, Staphylococcus aureus SA5, Listeria monocytogenes CCM4699 (inhibition zone sizing up to 20 mm). E. faecium 30E1 contained genes of four Ents; however, it showed no inhibition activity. Growth of the four indicators was inhibited due to the antimicrobial activity of E. faecium 32E1 with Ent P gene detection. This is the first study reporting on the occurrence of Ent genes in enterococci from Žinčica.

Synthesis, Characterisation, Crystal Structure and Anti-corrosion Studies of an Organotin(IV) Complex of 2-acetylpyridine 4-ethyl-3-thiosemicarbazone (LH): n-BuSn(L)Cl2

This study explored the possibility of Schiff-base ligand compound, 2-acetylpyridine 4-ethyl-3-thiosemicarbazone (LH) and its organotin(IV) complex (BuSn(L)Cl2) as a corrosion inhibitor for mild steel in 1M hydrochloric acid (HCl) medium. The chemical structures of the synthesised compounds were confirmed by performing elemental analysis, FT-IR, UV-Vis, NMR spectroscopy, and X-ray crystallography diffraction study. The structure showed the LH served as a tridentate (N, N’, S) donor to tin through its pyridyl, azomethine nitrogen, and thiolate sulphur. The corrosion inhibition characteristics of the free Schiff-base ligand and its organotin complex were studied by the standard weight loss method. They showed inhibition activity through adsorption, and this phenomenon was found to obey Langmuir adsorption isotherm. The inhibition efficiency of both compounds was found to increase as their concentration was increased from 1 to 3mM, but the efficiency achieved with the tin complex was greater, being 98.98% at 3mM concentration.

Meroterpenoids with diverse ring systems and dioxolanone-type secondary metabolites from Phyllosticta capitalensis and their phytotoxic activity

Twenty meroterpenoids with diverse ring systems including five new ones (1, 2, 5, 6, and 15), together with two new (23 and 24) and two known dioxolanone-type secondary metabolites were isolated from Phyllosticta capitalensis, an endophytic fungus from Cephalotaxus fortunei Hook. Compound 1 was the first example with a 9,14-seco ring A and a five-membered ring B in guignardone derivatives. Compound 2 represented a novel guignardone derivative possessing a 5/7/6/5 ring system with CH2-7 attached to C-4 rather than C-6 in ring D. The structures of all new compounds were elucidated using spectroscopic data analyses and electronic circular dichroism comparison. The phytotoxic effects of compounds 1–24 on Lactuca sativa and Lolium perenne were evaluated. Compound 22 showed inhibition activity on the shoots growth of L. sativa and L. perenne, as well as the roots growth of L. perenne.

Flavanoids derivatives from the root bark of Broussonetia papyrifera as a tyrosinase inhibitor

Materials developed from Broussonetia papyrifera have been widely used in food and paper industries, but this root bark of the plant has been overlooked despiteits potentially economic values. Thus, the aim of the present study was to develop the root bark of B. papyrifera as a source of bioactive compounds. Chemical investigation of the bark of B. papyrifera led to the isolation of nine flavonoids derivatives (1-9) and two coumarins (10-11), which includes five new compounds (1–4 and 11). The structures of isolates were elucidated based on spectroscopic analyses. All the isolates were evaluated for their biological effects including tyrosinase inhibition, cytotoxicity, and anti-inflammatory activity. Compound 3 showed inhibition activity against tyrosinase with an IC50 value of 9.29 μM (positive control, kojic acid: 30.56 μM). Molecular docking was used to support their inhibition activities which indicated that hydrogen bond formation played an important role in the ligand-enzyme complex. In addition, compound 4 exhibited cytotoxic activity against three cancer cell lines (NCI-H1975, HepG2, and MCF-7) and the structure-activity relationship of flavonoids (1-9) were established. Results from anti-inflammatory assay showed that compound 1 inhibited the production of IL-2 in Jurkat cells induced by phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA), with immunosuppressive activity. Overall, our study suggests that flavonoids from root bark of B. papyrifera can be used as a valuable source of bioactive compounds and support its use as a crop benefits to local economic.

Antidiabetic and Antioxidant Activities of Bay, Pandan, Citrus Leaves and Their Combination in Vitro

The study aimed to evaluate the effects of bay (B), pandan (P), citrus leaves (C) and their combinations against starch hydrolysis enzymes (α-glucosidase and α-amylase enzymes) and antioxidant activity and to examine the role of polyphenol compounds in enzyme inhibition and antioxidant activity. Three single leaves extracts and five of their combinations were applied to inhibit α-glucosidase hydrolyzing p-nitrophenyl-α-D-glucopyranosyde or α-amylase hydrolyzing starch solution as well as to scavenge free radicals. The leaf extracts and their combination showed inhibition activities against α-glucosidase and α-amylase enzymes with range of inhibition activities were between 17.63% to 26.04% and 20.14% to 35.30% respectively. There is no significantly differ among the extracts in modulation of α-glucosidase activity, but each extract exhibited different effect on α-amylase or antioxidant activities. Mixing P with B and C increases the inhibitory activity of the extract against α-amylase as seen that percent of inhibition of BPC is significantly higher than P, eventhough their total phenolic content was not different. The synergism or antagonism effect was not observed when the extracts were combined as the enzyme inhibition or antioxidant activities are not depend on the proportion of the extract in the mixtures. The role of polyphenol compounds on inhibition of the starch digestion enzymes and on antioxidant activity was not observed. Further study is required to fully elucidate the effect of the leaf or their combinations on diabetic animal models or diabetic patients.

Lactobacillus pentosus isolated from Muntingia calabura shows inhibition activity toward alpha-glucosidase and alpha-amylase in intra and extracellular level

Lactobacillus pentosus is one of the most prevalence lactic acid bacterium which available in plant-based fermentation. Here, we reported that the cell extract and cell-free supernatant of L. pentosus strain TL 2.7, TL 5.8 and TL 7.8 isolated from Kersen (Muntingiacalabura L.) showed potential anti-diabetic properties by inhibition of alpha-glucosidase and alpha-amylase activity in vitro. All isolated also enhanced the antioxidant capacity through DPPH in vitro test. This initial study will be beneficial for screening and application of functional food with anti-diabetic properties.

Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita

Abstract. Wiratno, Syakir M, Sucipto I, Pradana AP. 2019. Isolation and characterization of endophytic bacteria from roots of Piper nigrum and their activities against Fusarium oxysporum and Meloidogyne incognita. Biodiversitas 20: 682-687. The endophytic bacteria from spices plants have potential as biocontrol agents. Nevertheless, their potential has not been explored. Pepper plant (Piper nigrum L.) is linked with promising endophytic bacteria as biocontrol agents. This study aimed to obtain isolates of endophytic bacteria from root tissues of pepper plant. The isolation of endophytic bacteria was done using surface-sterilization method and using Tryptone Soya Agar (TSA) medium. The result showed that 10 endophytic bacteria were successfully isolated. We found that a total of 9 bacteria were safe for plants and mammals and used for subsequent steps. The selected bacteria showed inhibition activity of Fusarium oxysporum (18.6 to 43.7%). Furthermore, the secondary metabolites of the endophytic bacteria also promoted lethal effects on Meloidogyne incognita (16.6 to 65.8%). The physiological activity also showed that seven isolates were able to produce chitinase, and four (4) isolates were able to produce protease. Additionally, 55.6% of isolates were also able to dissolve phosphorus and fix nitrogen. This study provided fundamental information related to the biocontrol properties of endophytic bacteria isolated from pepper plant roots.