Short-term Experiments Research Articles

Intestinal flora variation reflects the short-term damage of microplastic to the intestinal tract in mice

The potential toxicity of microplastic (MPs) to organisms has attracted extensive attention. However, due to the subacute toxicity of MPs, the biological effect is hard to verify in short-term exposure experiment. Here, by tracking the dynamics of gut microbes, mice model was utilized to evaluate the toxicity of compositional MPs (PE, PET, PP, PS and PVC). After 7 days digestive exposure, the physiological indicators were normal as the control group that the body weight and serum cholesterol levels were insignificant change. Whereas, through histopathological examination, all the treatment groups suffered colon tissue damage, among which PS had the most inflammatory cells. Moreover, the high-throughput sequencing results revealed great variation of intestinal flora in treated mice. The ratio of Bacteroidetes and Firmicutes in PE, PET and PP treatment groups heighten, and the relative abundance of Ruminococcaceae and Lachnospiraceae increased significantly at family levels. At the genus level, Alistipes bacteria in PS treatment group significantly decreased that is associated with obesity risk. It indicated that MPs induced inflammatory response would further interfere the dynamics of intestinal flora causing health effect in living organisms. This work shed light on MPs toxicity in short-term exposure and supplied research paradigm of MPs health risk assessment.

Sleep postures monitoring based on capacitively coupled electrodes and deep recurrent neural networks

BackgroundCapacitively coupled electrode (CC electrode), as a non-contact and unobtrusive technology for measuring physiological signals, has been widely applied in sleep monitoring scenarios. The most common implementation is capacitive electrocardiogram (cECG) that could provide useful clinical information for assessing cardiac function and detecting cardiovascular diseases. In the current study, we sought to explore another potential application of cECG in sleep monitoring, i.e., sleep postures recognition.MethodsTwo sets of experiments, the short-term experiment, and the overnight experiment, were conducted. The cECG signals were measured by a smart mattress based on flexible CC electrodes and sleep postures were recorded simultaneously. Then, a classifier model based on a deep recurrent neural network (RNN) was proposed to distinguish sleep postures (supine, left lateral and right lateral). To verify the reliability of the proposed model, leave-one-subject-out cross-validation was introduced.ResultsIn the short-term experiment, the overall accuracy of 96.2% was achieved based on 30-s segment, while the overall accuracy was 88.8% using one heart beat segment. For the unconstrained overnight experiment, the accuracy of 91.0% was achieved based on 30-s segment, while the accuracy was 81.4% using one heart beat segment.ConclusionsThe results suggest that cECG could render valuable information about sleep postures detection and potentially be helpful for sleep disorder diagnosis.

Modeling nitrous oxide emissions from agricultural soil incubation experiments using CoupModel

Abstract. Efforts to develop effective climate mitigation strategies for agriculture require methods to estimate nitrous oxide (N2O) emissions from soil. Process-based biogeochemical models have been often used for field- and large-scale estimates, while the sensitivity and uncertainty of model applications to incubation experiments are less investigated. In this study, a process-oriented model (CoupModel) was used to simulate N2O and CO2 fluxes and soil mineral nitrogen (N) contents in a short-term (43 d) factorial incubation experiment (16 treatments). A global sensitivity analysis (GSA) approach, “Morris screening”, was applied to quantify parameter sensitivity. The GSA suggested that a higher number of sensitive parameters was associated with N2O flux estimates and that inter-treatment variations in parameter sensitivities were distinguished by soil moisture levels or NO3- content and residue types. Important parameters regarding N2O flux estimates were linked to the decomposability of soil organic matter (e.g., organic C pool sizes) and the denitrification process (e.g., Michaelis constant and denitrifier respiratory rates). After calibration, the model better captured temporal variations and magnitude of gas fluxes and mineral N in unamended soils than in residue-amended soils. Low-magnitude daily and cumulative N2O fluxes were well simulated with mean errors (MEs) close to zero, but the model tended to underestimate N2O fluxes, as observed daily values increased by over 0.1 g N m−2 d−1, in which the major mismatch was due to limited success of the model to describe the high emissions during the first few days after crop residue addition. A larger uncertainty was also seen in the magnitude of pulse emissions by the posterior simulations. We also evaluated ancillary variables regarding N cycling, which indicated that more frequent measurements and additional types of observed data such as soil oxygen content and the microbial sources of emitted N2O are required to further evaluate model performance and biases. The major challenges for calibration were associated with high sensitivities of denitrification parameters to initial soil abiotic conditions and the instantaneous residue amendment. Model structure uncertainties and improved modeling practices in the context of incubation experiments were discussed.

DFT Analysis of Ethanol Electro-Oxidation on Fe(110) and Fe3c(110) and Its Correlation with the Stress Corrosion Cracking of Carbon Steel

Stress Corrosion Cracking (SCC) of carbon steel in fuel-grade ethanol is typically intergranular in service, but it is nearly always transgranular, in short-term laboratory experiments. While this might be considered an inconvenience, it provides an important clue about the SCC mechanism. The important point is that the transgranular cracking occurs under much milder mechanical conditions than any known form of hydrogen embrittlement in ordinary carbon steel. For this and other reasons, hydrogen is not considered to be the cause of this form of SCC. Transgranular SCC of carbon steel occurs in a very particular set of environments. Anhydrous liquid ammonia, anhydrous ammonia-methanol and CO-CO2-H2O are the key systems to be considered, apart from alcohols. High-temperature water containing oxygen also causes transgranular SCC, but is a less severe environment, probably because a magnetite film imposes a compressive stress that partially opposes any embrittlement effect. In our view, the important factor connecting these examples is embrittlement by interstitials. Just as hydrogen can embrittle iron, so can nitrogen or carbon (or oxygen). The main distinction is the diffusivity of the interstitial in iron. Ammonia can be oxidized to N, and CO can be reduced to C (or, in the language of surface chemistry, this could be dissociative adsorption). Still, at ambient temperature, those interstitials can only diffuse a few nm in relevant times of seconds. But we know little about the effect of such near-surface embrittlement on the propagation of a crack, and research on such surface effects is very important. Striking mechanical and morphological similarities of the SCC of carbon steel in ethanolic media with those governed by a cleavage-like mechanism in CO-CO2 aqueous solutions prompted the investigation of the possibility of ethanol electrochemical oxidation into CO on ferrite (Fe) and cementite (Fe3C) surfaces. Density functional theory computations on (110) surfaces revealed that the catalytic activity of Fe and Fe3C through the α dehydrogenation pathway can significantly reduce the energy barrier of electro-oxidation of ethanol and production of CO to 0.575 and 0.480 eV, respectively. These first principle calculations indicate that at the anodic potentials applied during potentiostatic slow strain rate testing, ethanol electro-oxidation to CO is thermodynamically viable on carbon steel, giving further credit to the involvement of cleavage type SCC of carbon steel in ethanolic environments.

Historical Antibiotic Stress Changed the Effects of Sulfamethoxazole and Trimethoprim on Activated Sludge: ARGs and Potential Hosts

The co-exposure of antibiotics has important effects on antibiotic resistance genes (ARGs) and microbial community aggregation in wastewater treatment plants (WWTPs). However, it is unclear whether differences in historical antibiotic exposure stress can determine responses of microbes and ARGs to combined antibiotics. By selecting a high concentration (30 mg·L-1) of sulfamethoxazole (SMX) and trimethoprim (TMP) as historical exposure stress conditions, the effects of SMX and TMP-combined pollution on ARGs, bacterial communities, and their interactions were explored in short-term experiments. Based on high-throughput quantitative PCR, a total of 13 ARGs were detected, and the absolute abundance was 2.21-5.42 copies·μL-1 (logarithm, DNA, the same below). Among them, sul2, ermB, mefA, and tetM-01 were the main subtypes in the samples, and the absolute abundance was between 2.95 and 5.40 copies·μL-1. The combined contamination of SMX and TMP could cause the enrichment of ARGs and mobile genetic elements (MGEs); however, their effects on each subtype were different, and the historical legacy effect of SMX was higher than that of TMP. Under the different exposure histories, the co-occurrence and co-exclusion patterns existed between ARGs. Moreover, MGEs (especially intI-1) were significantly correlated with sulfonamides (sul1 and sul2), tetracyclines[tet(32)], and macrolide-lincosamide-streptogramin (MLSB) resistance genes (ermB). Based on the full-scale classification of microorganisms, it was found that the microbial community structure of various groups responded differently to combined pollution, and the conditionally abundant taxa (CAT) were obviously enriched. Thauera, Pseudoxanthomonas, and Paracoccus were the dominant resistant bacterial genera. Furthermore, a total of 31 potential hosts of ARGs were identified with network analysis, which were dominated with conditionally rare taxa (CRT). Particularly, Candidatus_Alysiosphaera and Fusibacter were positively correlated with most of the ARGs, being the common protentional hosts. Importantly, some rare genera (RT, Variibacter, Aeromonas, Cloacibacterium, etc.) were potential hosts of transposon IS613, which played an important role in the proliferation and spread of ARGs. In conclusion, this study revealed the legacy effects of historical antibiotic stress on ARGs and their hosts, which could provide new ideas and theoretical basis for reducing ARGs pollution in WWTPs.

Experimental methodologies to perform accelerated deactivation studies of hydrotreating catalysts

A comprehensive review of the scientific literature over the last two decades is conducted to have a clear understanding of the factors that accelerate the deactivation of hydrotreating (HDT) catalysts. The reported experimental methodologies employed at laboratory and pilot plant scales focused on achieving accelerated deactivation of HDT catalysts are reviewed and discussed. The different methodologies attempt to reproduce industrial long-term catalysts deactivation by using different operating conditions (mainly high reaction temperature and low H2-to-oil ratio) or feedstock with high content of compounds that cause coke and metal deposition, in short-term experiments.

Effect of Short-Term Phosphorus Supply on Rhizosphere Microbial Community of Tea Plants

Microbes play an important role in rhizosphere phosphorus (P) activation and root P absorption in low P-available soils. However, the responses of the rhizosphere microbial community to P input and its effects on P uptake by tea plants have not been widely reported. In this study, the high-throughput sequencing of the 16S rRNA gene and the ITS2 region was employed to examine the responses of tea rhizosphere microbiomes to different P input rates (low-P, P0: 0 mg·kg−1 P; moderate-P, P1: 87.3 mg·kg−1 P; high-P, P2: 436.5 mg·kg−1 P). The results showed that the P input treatments significantly reduced the soil C: N ratio and C: P ratio compared to the P0 treatment (p < 0.05). Moreover, the P2 treatment significantly increased the soil available P, plant biomass and P content of the tea plant compared to the P0 and P1 treatments (p < 0.05). Both bacterial and fungal communities revealed the highest values of alpha diversity indices in the P1 treatment and the lowest in the P2 treatment. The dominant phyla of the bacterial community were Proteobacteria, Actinobacteria and Acidobacteria, while in the fungal community they were Ascomycota and Mortierellomycota. In addition, P input enriched the relative abundance of Actinobacteria and Proteobacteria but decreased the relative abundance of Acidobacteria. The Mantel correlation analysis showed that the fungal community was influenced by P input, whereas bacterial community was affected by the soil TC and C: N ratio. Furthermore, the P input treatments enhanced the TCA cycle, amino and nucleotide glucose metabolism, starch and sucrose metabolism, and phosphotransferase system expression, which could promote C and N cycling. On the contrary, the P input treatments negatively affected the growth of arbuscular mycorrhizal fungi. The PLS-PM model revealed that the rhizosphere bacterial and fungal communities, respectively, negatively and positively affected the P content by affecting the biomass. Meanwhile, rhizosphere microbial function profiles affected the P content of tea plants directly and positively. In summary, moderate P input favors the rhizosphere microbial diversity and functions in the short-term pot experiment. Therefore, we suggest that moderate P input should be recommended in practical tea production, and a further field test is required.

Innovative geocomposite for dredged sediments depollution

In France, sediments from river dredging are considered as waste. Without any special treatment, they must be stored in landfills. The dredged sediments of our area of study (North of France) are polluted with trace metals (antimony and zinc are above the French regulation threshold). The main objective of this study is to develop a new method of active depollution and dewatering treatment in order to enable the reuse of treated sediments. This treatment is carried out through ponds equipped with innovative geocomposite. This innovative geocomposite has two typical passive functions (filtration and drainage). In addition, it is functionalized by an innovative electrokinetic technology to confer it active roles (depollution and dewatering). Electrokinetic consists in applying a difference of electrical potential to a porous medium. We present here the results of a preliminary laboratory study that simulates this new method on a short-term (3 weeks) experiment. The results show that the electrokinetic treatment has an impact on the chemical and physical properties of sediments. The treatment reduces by 50% the concentration of zinc in the leachate after lixiviation.

Ultramicroelectrode Based Approaches to Diagnose Utility of Redox Electrolytes in Flow Batteries

The proposed redox electrolyte system, concentrated solutions of Keggin type silicotungstic acid (H4SiW12O40), have been tested using the microelectrode methodology to determine mass-transport (effectively diffusional) coefficients for charge propagation and homogeneous (electron self-exchange) rates of electron transfers. Silicotungstic acid (H4SiW12O40) acts as proton conductors, and undergoes fast, reversible, multi-electron electron transfers leading to the formation of highly conducting, mixed-valence (tungsten(VI,V) heteropoly blue) compounds. To develop useful electroanalytical diagnostic criteria, electroanalytical approaches utilizing ultramicrodisk electrodes have been adapted to characterization of redox electrolytes. Combination of two parallel ultramicroelectrode-based experiments performed under radial (long-term experiment; e.g., slow scan rate) and linear (short-term experiment; e.g., fast scan rate) diffusion conditions permits determination of both effective concentration of redox centers and transport (diffusion) coefficient parameters, which are crucial to evaluation of the utility of a redox electrolyte for charge storage.

TOXICOLOGICAL AND HYGIENIC ASSESSMENT OF THE NEW ACTIVE INGREDIENT OF THE AVERSECTINS CLASS - MILBEMECTIN AND THE FORMULATION BASED ON IT MILBEKNOK 1%, CE

Relevance. The priorities of modern agricultural production are the application of new pesticides that are highly effective, safe for humans and do not have a negative impact on environmental objects. The most promising is the synthesis of substances from natural materials or their synthetic analogues (pesticides of biological origin). These include avermectin class substance – milbemectin.
 Objective of the work was the toxicological and hygienic evaluation of the new active ingredient of the aversectins class - milbemectin and the formulation based on it Milbeknok 1%, CE, substantiation of the acceptable daily dose (ADD) of milbemectin.
 Materials and methods. Expert-analytical, toxicological and hygienic methods, analysis of literature data were used during the research. The hygienic assessment of the toxicity of milbemectin and the formulation based on it was carried out in accordance with State Standard 8.8.1.002-98 “Hygienic classification of pesticides by degree of hazard”.
 Results. The analysis of the acute toxicity of the formulation and its active ingredient showed that Milbeknok 1%, CE, according to State Standard 8.8.1.002-98, belongs to the 3rd class of hazard, and milbemectin – to the 2nd class, according to the limiting criterion – acute inhalation toxicity.
 In short-term and long-term experiments with rats, mice and dogs, the main effect of milbemectin exposure was general toxicity and hepatotoxicity. According to carcinogenicity, reproductive and embryotoxicity, milbemectin is pertained to the 3rd class of hazard.
 Substantiating the ADD of milbemectin we used the smallest NO(A)EL value of 3 mg/kg, established for the general toxic effect in the experiment to study the chronic effect on dogs and safety factor of 1000 (taking into account the lack of a study of developmental neurotoxicity and limited data on reproductive toxicity of the compound): ADD = 3/1000 = 0.003 mg/kg.
 Conclusions. It was established that the Milbeknok 1%, CE pertains to the 3rd class, and its active substance - to the 2nd class of hazard, according to the limiting criterion – acute inhalation toxicity. The substantiated ADD value of milbemectin is 0.003 mg/kg.

Co-composting of cattle manure with biochar and elemental sulphur and its effects on manure quality, plant biomass and microbiological characteristics of post-harvest soil.

Improvement of manure by co-composting with other materials is beneficial to the quality of the amended soil. Therefore, the manure was supplied with either biochar, elemental sulphur or both prior to fermentation in 50 L barrels for a period of eight weeks. The manure products were subsequently analyzed and used as fertilizers in a short-term pot experiment with barley fodder (Hordeum vulgare L.). The experiment was carried out under controlled conditions in a growth chamber for 12 weeks. The sulphur-enriched manure showed the lowest manure pH and highest ammonium content. The co-fermentation of biochar and sulphur led to the highest sulphur content and an abundance of ammonium-oxidizing bacteria in manure. The biochar+sulphur-enriched manure led to the highest dry aboveground plant biomass in the amended soil, whose value was 98% higher compared to the unamended control, 38% higher compared to the variant with biochar-enriched manure and 23% higher compared to the manure-amended variant. Amendment of the sulphur-enriched manure types led to the highest enzyme activities and soil respirations (basal, substrate-induced). This innovative approach to improve the quality of organic fertilizers utilizes treated agricultural waste (biochar) and a biotechnological residual product (elementary sulphur from biogas desulphurization) and hence contributes to the circular economy.

Responses of Mytilus galloprovincialis to challenge with environmental isolates of the potential emerging pathogen Malaciobacter marinus

Bacteria of the Arcobacter-like spp. represent emerging foodborne zoonotic pathogens in humans and animals. Their increasing presence in seafood, suggesting higher occurrence in seawater due to marine pollution, is raising some environmental concern. Although Arcobacter is frequently detected in diseased oysters and stressed bivalve species, no data are available so far on its potential pathogenicity or interactions with the immune system of the bivalve host.In this work, responses to challenge with two strains of Malaciobacter marinus IRTA-19-131 and IRTA-19-132, R1 and R2), isolated from adult Crassostrea gigas during a mortality event in 2019 in Spain, were investigated in the mussel Mytilus galloprovincialis.In vivo experiments were performed in larvae (48 h post-fertilization), and in adult mussels at 24 h post-injection, in order to evaluate the pathogenicity for early developmental stages, and the hemolymph immune responses, respectively. Both R1 and R2 were moderately pathogenic to early larvae, with significant decreases in the development of normal D-veligers from 104 and 103 CFU/mL, respectively. In adults, both strains decreased hemocyte lysosomal membrane stability (LMS), and stimulated extracellular defense responses (ROS production and lysozyme activity).The interactions between mussel hemocytes and M. marinus were investigated in in vitro short-term experiments (30–90 min) using the R1 strain (106–108 CFU/mL). R1 decreased LMS and induced lysosomal enlargement, but not cell detachment or death, and stimulated extracellular ROS production and lysozyme release, confirming in vivo data. Moreover, lysosomal internalization and degradation of bacteria were observed, together with changes in levels of activated mTor and LC3, indicating phagocytic activity. Overall, the results indicate the activation of both extracellular and intracellular immune defenses against M. marinus R1. Accordingly, these responses resulted in a significant hemolymph bactericidal activity, with a large contribution of hemolymph serum.The results represent the first data on the potential pathogenicity of Arcobacter isolated from a shellfish mortality to bivalve larvae and adults, and on their interactions with the immune system of the host.

S6.3c Adaptive dynamics in experimental populations of Aspergillus nidulans

S6.3 Fungal adaptation and evolution, September 22, 2022, 4:45 PM - 6:15 PM A total of 12 replicate populations initiated with a laboratory strain of the ascomycete fungus Aspergillus nidulans evolved on synthetic minimal glucose agar medium for 1 year, using weekly transfers of 1% of the produced asexual spores to fresh medium. This Aspergillus short-term evolution experiment (ASEX) was designed to understand how filamentous fungi adapt to growth on limited carbon in a spatially structured environment. We observed no systematic improvement in the fitness components tested and neither in the competitive fitness relative to the ancestor. Instead, we observed the repeated evolution of at least two morphotypes, with a fluffy-like (FL) or an ancestor-like (AL) colony morphology, leading to non-transitive fitness interactions among isolates in two selected populations. The genomic analyses of clones from all 12 populations at an early (week 10) and the final time point (week 52), show a clear role of natural selection during ASEX. We also observed a shared genetic basis and different timing of adaptation of AL and FL types. In addition, in most populations, both morphotypes do not form monophyletic groups, but they frequently disappear and re-evolve from ancestral forms of both types. Reduction in asexual spore yield, the most evident parallel phenotypic change found in all our evolved populations, is not due to the direct selection of genes involved in asexual reproduction. Instead, we argue that reduced spore yield is a pleiotropic effect of adaptive changes in metabolism.

Continuous Formation of Limonene Carbonates in Supercritical Carbon Dioxide.

We present a continuous flow method for the conversion of bioderived limonene oxide and limonene dioxide to limonene carbonates using carbon dioxide in its supercritical state as a reagent and sole solvent. Various ammonium- and imidazolium-based ionic liquids were initially investigated in batch mode. For applying the best-performing and selective catalyst tetrabutylammonium chloride in continuous flow, the ionic liquid was physisorbed on mesoporous silica. In addition to the analysis of surface area and pore size distribution of the best-performing supported ionic liquid phase (SILP) catalysts via nitrogen physisorption, SILPs were characterized by diffuse reflectance infrared Fourier transform spectroscopy and thermogravimetric analysis and served as heterogeneous catalysts in continuous flow. Initially, the continuous flow conversion was optimized in short-term experiments resulting in the desired constant product outputs. Under these conditions, the long-term behavior of the SILP system was studied for a period of 48 h; no leaching of catalyst from the supporting material was observed in the case of limonene oxide and resulted in a yield of 16%. For limonene dioxide, just traces of leached catalysts were detected after reducing the catalyst loading from 30 to 15 wt %, thus enabling a constant product output in 17% yield over time.

Promotion of microwave discharge over carbon catalysts for CO2 reforming of CH4 to syngas

The discharge (microplasma) of carbon materials in microwave fields can activate reaction molecules and promote the reaction process. This paper studied the microwave discharge characteristics under different carbon materials, particle sizes, particle structures, and microwave electric/magnetic fields. Combining microwave short-term pulse reaction experiments and spectroscopic experiments, the effect of discharge on reforming reactions was analyzed. The results show that: different carbon materials had different discharge intensities, but they all produced star-shaped sparks. Particles with cis-fiber structure and tips placed in opposition to each other were easier to discharge in microwave electric fields. In the same type of activated carbon, the 0.6 mm coconut shell activated carbon has many discharge sites and high discharge frequency, so the reaction effect was the best. The reactant conversions reached more than 80 %, the syngas yield was 92 %, and the H2/CO was about 1.1. The discharge could promote the activation and decomposition of reaction gas and improve the conversion of reactants.

Persistence of phenotypic responses to short-term heat stress in the tabletop coral Acropora hyacinthus.

Widespread mapping of coral thermal resilience is essential for developing effective management strategies and requires replicable and rapid multi-location assays of heat resistance and recovery. One- or two-day short-term heat stress experiments have been previously employed to assess heat resistance, followed by single assays of bleaching condition. We tested the reliability of short-term heat stress resistance, and linked resistance and recovery assays, by monitoring the phenotypic response of fragments from 101 Acropora hyacinthus colonies located in Palau (Micronesia) to short-term heat stress. Following short-term heat stress, bleaching and mortality were recorded after 16 hours, daily for seven days, and after one and two months of recovery. To follow corals over time, we utilized a qualitative, non-destructive visual bleaching score metric that correlated with standard symbiont retention assays. The bleaching state of coral fragments 16 hours post-heat stress was highly indicative of their state over the next 7 days, suggesting that symbiont population sizes within corals may quickly stabilize post-heat stress. Bleaching 16 hours post-heat stress predicted likelihood of mortality over the subsequent 3-5 days, after which there was little additional mortality. Together, bleaching and mortality suggested that rapid assays of the phenotypic response following short-term heat stress were good metrics of the total heat treatment effect. Additionally, our data confirm geographic patterns of intraspecific variation in Palau and show that bleaching severity among colonies was highly correlated with mortality over the first week post-stress. We found high survival (98%) and visible recovery (100%) two months after heat stress among coral fragments that survived the first week post-stress. These findings help simplify rapid, widespread surveys of heat sensitivity in Acropora hyacinthus by showing that standardized short-term experiments can be confidently assayed after 16 hours, and that bleaching sensitivity may be linked to subsequent survival using experimental assessments.

The bulk parameterizations of turbulent air–sea fluxes in NEMO4: the origin of sea surface temperature differences in a global model study

Abstract. Wind stress and turbulent heat fluxes are the major driving forces that modify the ocean dynamics and thermodynamics. In the Nucleus for European Modelling of the Ocean (NEMO) ocean general circulation model, these turbulent air–sea fluxes (TASFs) can critically impact the simulated ocean characteristics. This paper investigates how the various bulk parameterizations used to calculate turbulent air-sea fluxes in NEMOv4 can lead to substantial differences in the estimation of sea surface temperatures (SSTs). Specifically, we study the contributions of different aspects and assumptions of the bulk parameterizations in driving the SST differences in the NEMO global model configuration at 1/4∘ of horizontal resolution. These aspects include the use of the skin temperature instead of the bulk SST in the computation of turbulent heat flux components and the estimation of wind stress and turbulent heat flux components, which vary in each parameterization due to different bulk transfer coefficients. The analysis of a set of short-term sensitivity experiments where the only change is related to one of the aspects of the bulk parameterizations shows that parameterization-related SST differences are primarily sensitive to wind stress differences and to the implementation of skin temperature in the computation of turbulent heat flux components. In addition, in order to highlight the role of SST–turbulent heat flux negative feedback at play in ocean simulations, we compare the TASF differences obtained using the NEMO ocean model with the estimations by Brodeau et al. (2017), who compared the different bulk parameterizations using prescribed SSTs. Our estimations of turbulent heat flux differences between bulk parameterizations are weaker than those found by Brodeau et al. (2017).

Sulfate availability and soil selenate adsorption alleviate selenium toxicity in rice plants

Selenium (Se) is a micronutrient for humans and other animals; however, it can cause severe toxicity at high concentrations. Selenium and sulfur (S) present a strict relationship in plants and soils, affecting their uptake and accumulations. Thus, addressing selenium-sulfur interaction is important to understand selenium nutrition and toxicity in plants, which could influence crop composition and production, and the toxicity risk to humans and animals. Here, we aimed to evaluate the impact of selenium exposure on rice plants grown under different sulfur supplies at tillering and grain ripening phases. We studied the effects of selenate and sulfate doses on rice plants grown hydroponically or in soils with varying clay contents. In the hydroponic experiment (short-term experiment), rice plants were grown until tillering stage under combinations of two concentrations of Se (0 and 20 µM Se) and S (0.1 and 0.5 mM S) in the nutrient solution. In the long-term, rice plants were grown until the ripening stage in two Oxisols with different clay contents (240 and 620 g kg−1 clay) under combinations of five doses of Se (0; 0.5; 1.0; 2.0 and 4.0 mg dm−3 Se) and three doses of S (0; 45; and 90 mg dm−3 S). We also performed sorption assays to evaluate the influence of soil clay content (240 and 620 g kg−1 clay) and S doses (0; 45; and 90 mg dm−3 S) on selenate adsorption and desorption at exposure to 4.0 mg kg−1 Se. Sulfate supply alleviated selenate toxicity in both short-term and long-term experiments. Selenate treatment up-regulated the expression of sulfate transporters (OsSULTR1;1 and OsSULTR1;2), leading to increased sulfur contents in rice seedlings, which enhanced the antioxidant system (catalase and ascorbate peroxidase activities and glutathione content) and alleviated selenate toxicity. However, this enhanced mechanism is absent in seedlings grown under a low sulfur supply, presenting severe Se toxicity. Moreover, soil clay contents strongly influenced selenate availability. The higher clay content promoted a high selenate adsorption capacity (67% of Se added), resulting in lower selenium contents in shoots and grains and the absence of toxicity symptoms. In contrast, a lower clay content presented a low selenate adsorption capacity (24% of Se added), increasing the Se availability, which can favor the biofortification of crops. However, high selenate doses caused growth and yield impairment in rice cultivated in the soil with lower selenate adsorption, which exhibited higher Se concentrations in shoots and grains, increasing the risk of Se toxicity for humans and animals.

Ebullition-facilitated mobilization of trapped dense non-aqueous phase liquid at residual saturation from sandy sediments.

Gas ebullition can mobilize dense non-aqueous phase liquids (DNAPLs) from sediments to the overlying water column, increasing the DNAPL-impacted area and posing serious challenges to the remediation and management of contaminated sediments. Despite this, there have been few laboratory studies focused on gas ebullition-facilitated transport of DNAPL. In this study, bubble-facilitated transport was investigated by injecting gas (air or nitrogen) at 1mL/min through a creosote source zone (∼25% saturation) capped with sand layers of different thicknesses. Three short-term experiments (8.3-8.7h) were capped with 11.4, 7.0 or 4.5cm of sand to estimate DNAPL flux. One long-term experiment (30 days) was capped with 8cm of sand to investigate DNAPL removal. Heptane placed on a layer of water above the sand was used as a solvent trap and analyzed for petroleum hydrocarbons (PHCs). Results showed that creosote travelled as thin coatings and films surrounding gas bubbles migrating out of the source zone. Gas invasion was dominated by capillarity in the 11.4cm-thick sand layer and by fracturing in the 7.0 and 4.5cm-thick sand layers. Migration through these fractures often led to the formation of creosote tails on mobilized bubbles that drained towards the rear end of the bubble. The mass released decreased exponentially with sand cap thickness. In the long-term experiment, images showed significant depletion of the source zone in 30 days. Linear regression analysis showed that relationships with high predictive capabilities for ebullition-facilitated fluxes of hydrophobic organic contaminants can be obtained by incorporating gas ebullition flux and source strength, based on results from this study along with others from the field and laboratory. To our knowledge, this is the first study to compile and integrate data collected from laboratory and field studies to develop an assessment tool to facilitate the management of contaminated sediments affected by gas ebullition.

A new catheter-integrated drug-delivery system for controlled intravesical mitomycin C release

Intravesical treatment of bladder cancer is preferred over systemic administration. However, the efficacy of intravesical instillations is challenged by the periodic voiding that flushes out the instilled drug and ultimately reduces drug exposure to the bladder epithelium. Here, we demonstrate a new catheter-integrated drug-delivery concept that utilizes a silicone-based interpenetrating polymer network (IPN) as material for the catheter balloon, to facilitate continuous release of the bladder cancer adjuvant, Mitomycin C, from a balloon-reservoir to the urinary bladder. Long-term release properties and anti-carcinoma cell efficacy of released drug was investigated in vitro. Short-term release experiments were performed in live pigs to evaluate the IPN prototype catheter in a physiological relevant environment in vivo. Sustained zero-order release of Mitomycin C was achieved for 12 days in vitro without refilling the balloon. Mitomycin C was released from the IPN-balloons into the urinary bladder of live pigs in concentrations adequate to inhibit carcinoma cell growth. The IPN catheter represents a new drug-delivery concept for prolonged Mitomycin C delivery to the urinary bladder.