Background: The ankle joint is among the most vulnerable areas for injuries during daily activities and sports. This study focuses on individuals with chronic ankle instability (CAI), comparing the biomechanical characteristics of the lower limb during side-step cutting under various conditions. The aim is to analyze the impact of kinesiology tape (KT) length on the biomechanical properties of the lower limb during side-step cutting, thereby providing theoretical support and practical guidance for protective measures against lower-limb sports injuries. Methods: Twelve subjects with CAI who met the experimental criteria were recruited. Each subject underwent testing without taping (NT), with short kinesiology tape (ST), and with long kinesiology tape (LT), while performing a 45° side-step cutting task. This study employed the VICON three-dimensional motion capture system alongside the Kistler force plate to synchronously gather kinematic and kinetic data during the side-step cutting. Visual 3D software (V6.0, C-Motion, Germantown, MD, USA) was utilized to compute the kinematic and kinetic data, while OpenSim 4.4 software (Stanford University, Stanford, CA, USA) calculated joint forces. A one-way Analysis of Variance (ANOVA) was conducted using SnPM, with the significance threshold established at p < 0.05. The Origin software 2021 was used for data graphic processing. Results: KT was found to significantly affect joint angles, angular velocities, and moments in the sagittal, frontal, and transverse planes. LT increased hip and knee flexion angles as well as angular velocity, while ST resulted in reduced ankle inversion and increased knee internal rotation. Both types of KT enhanced hip abduction moment and knee adduction/abduction moment. Additionally, LT reduced the ankle joint reaction force. Conclusions: These findings suggest that the application of KT over a short duration leads to improvements in the lower-limb performance during side-step cutting motions in individuals with CAI, thus potentially decreasing the risk of injury.
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