Short Life Research Articles

PELATIHAN PENGOLAHAN “PAYA BITES”, CEMILAN SEHAT BERBAHAN PEPAYA, BAGI ANGGOTA FKIF UNNES

<em>Papaya is a seasonal fruit that is rich in water content and has a relatively short shelf life. Nowadays, people's interest in consuming fruit directly after it is ripe is relatively low because some of them don't like fruit. For this reason, it is necessary to innovate fruit-based products, as an effort to overcome the problem of lack of fruit consumption. This article discusses "PAYA BITES", an entrepreneurial venture that presents papaya crackers as an innovative snack product made from papaya with a variety of contemporary flavors. In this activity, the method used was training (socialization) aimed at 41 members of the Islamic Physics Study Forum (FKIF) at Semarang State University. This activity aims to be a form of education and training for FKIF UNNES members in processing papaya fruit into papaya crackers and has the potential to be marketed to the general public. The result of this training is to provide knowledge and convenience for FKIF UNNES members in processing papaya fruit into papaya crackers which have so far been underutilized so that they become a more valuable commodity</em>

Effects of Drying Methods on the Phytochemical Contents, Antioxidant Properties, and Anti-Diabetic Activity of Nasturtium officinale R.Br. (Betong Watercress) from Southern Thailand.

Nasturtium officinale R.Br. (Betong watercress) contains high levels of secondary metabolites that offer health benefits. However, fresh-cut watercress has a short shelf life. This study aimed to assess the effect of drying methods on the phytochemical contents, antioxidant activity, and anti-diabetic activity of Betong watercress. The watercress was dried using three methods: roasting (R, 50 °C, 40 min); roasting and drying (RD, 40 min roasting at 50 °C and 1 h drying at 80 °C); and blanching, roasting, and drying (BRD, 30 s blanching at 80 °C, 20 min roasting at 50 °C, and 1 h drying at 80 °C). Aqueous extracts from each drying method were analyzed for total phenolic content, total flavonoid content, total glucosinolate content, antioxidant activities (FRAP, DPPH, and ABTS assays), and α-amylase enzyme inhibition. From the results, the R method provided the highest level of total phenolic, total flavonoid, and total glucosionolate content compared to the RD and BRD methods. Similarly, antioxidant activities and α-amylase enzyme inhibition were highest in the R method, followed by the RD and BRD methods. Our results demonstrate that roasting of Betong watercress without the addition of blanching or drying effectively preserves the phytochemical contents, antioxidant activities, and anti-diabetic activity.

Implementing Optogenetic-Controlled Bacterial Systems in Drosophila melanogaster for Alleviation of Heavy Metal Poisoning.

Drosophila melanogaster (fruit fly) is an animal model chassis in biological and genetic research owing to its short life cycle, ease of cultivation, and acceptability to genetic modification. While the D. melanogaster chassis offers valuable insights into drug efficacy, toxicity, and mechanisms, several obvious challenges such as dosage control and drug resistance still limit its utility in pharmacological studies. Our research combines optogenetic control with engineered gut bacteria to facilitate the precise delivery of therapeutic substances in D. melanogaster for biomedical research. We have shown that the engineered bacteria can be orally administered to D. melanogaster to get a stable density of approximately 28,000 CFUs/per fly, leading to no detectable negative effects on the growth of D. melanogaster. In a model of D. melanogaster exposure to heavy metal, these orally administered bacteria uniformly express target genes under green light control to produce MtnB protein for binding and detoxifying lead, which significantly reduces the level of oxidative stress in the intestinal tract of Pb-treated flies. This pioneering study lays the groundwork for using optogenetic-controlled bacteria in the model chassis D. melanogaster to advance biomedical applications.

Optimization of High-Pressure Processing for Microbial Inactivation in Pigmented Rice Grass Juice and Quality Impact Assessment during Refrigerated Storage.

Pigmented rice grass juice (RGJ) is a good source of bioactive compounds, but fresh juice has a relatively short shelf life of only 7 days at 4 °C. The objectives of this study were to determine the optimal growth stage of pigmented rice grass, investigate the optimal condition of high-pressure processing (HPP) for bacterial inactivation in inoculated RGJ using response surface methodology (RSM), and evaluate quality changes in uninoculated HPP-treated juice during storage at 4 °C compared with heat-treated (85 °C/10 min) and untreated samples. Results revealed that the optimal growth stage of rice grass was 9 days with the highest total anthocyanin content of 158.92 mg/L. The optimal condition of HPP was determined to be 612 MPa, 11 min, and 36 °C, and inactivated Escherichia coli K12 and Listeria innocua with 6.43 and 5.02 log reductions, respectively, meeting FDA regulations. The lethality of bacteria after HPP treatment can be explained by damage to the cell membrane and the leakage of intracellular constituents such as protein and nucleic acid. During 12 weeks of storage at 4 °C, total plate counts and yeast and mold counts in uninoculated HPP-treated juice were not detected. Moreover, HPP did not significantly change phytochemical properties (p < 0.05), caused a minor impact on physicochemical properties of RGJ, and maintained the durability of juice samples during storage. Analysis of the phytochemical profile revealed that HPP treatment could preserve most of the phenolic compounds in RGJ and especially increase the contents of protocatechuic acid, 4-hydroxybenzoic acid, syringic acid, transcinnamic acid, isorhamnetin-3-o-glucoside, quercetin, and cyanidin-3-glucoside (p < 0.05). Overall, HPP is a potential pasteurization technique for microbial inactivation and nutritional preservation for rice grass juice.

Some Observations on the Strength of Viking Ship Rudders

Technical papers which attempt to analyse historic and ancient ship structures using modern techniques appear only spasmodically in today’s learned engineering journals. There could be many good reasons for this; the lack of commercial incentives, the paucity of accurate engineering data and perhaps a certain reluctance to cross into an area which is rightly the province of the nautical archaeologist. This, in the author’s view is a pity since simplified analytical techniques of the type routinely used in design offices can offer useful cost-effective insights into the physical behaviour of historic ships and craft, without endangering the physical reconstruction or its crew. In addition as the input data are often only loosely defined, less sophisticated methods are ideal for conducting “data-sensitivity” investigations. One such paper (Loscombe, 2022) described efforts to apply modern local and global structural design methods to Viking-age ships with a view to establishing plausible operational factors of safety for comparison with modern requirements. This follow-up contribution continues with the theme by focusing on the rudder. Rudder failure can endanger any ship, ancient, medieval or modern but the Viking ship rudder has a number of structural features not found on modern vessels which invite retrospective stress analyses. One component in particular, the lower bearing in modern terminology, appears to have a very short operational life, measured in days rather than years as is the expectation for today’s marine vehicles and hence the Viking ship rudder is a good candidate for such simplified numerical analyses.

Application of Response Surface Methodology Experimental Design to Optimize Tribological Lubrication Characteristics

Total Knee Replacement (TKR) has become a standard operation for patients with joint disorders. Despite the fact that the number of procedures is increasing all the time, the short service life of implants remains a persistent concern for researchers. Understanding lubrication may aid in explaining tribological processes that lead to replacements that last well into the third decade of service. Likewise, wear and friction in total knee replacement (TKR) components are among the most common causes of implant failure. As a result, this study will evaluate the feasibility of using the polymer Poly Lactic Acid (PLA) for cartilage replacement in Total Knee Replacement (TKR) using plant-based oils as lubricants. Furthermore, the modifier will be added to plant-based oils as an additive to make them analogous to human bodily fluids. The present paper is applying the Box-Behnken design to optimize the performance and mechanical responses of bio-lubricants toward Poly Lactic Acid (PLA) as a tibial insert for cartilage replacement in Total Knee Replacement (TKR). The main objective of this paper is to develop an optimized method for the selection of plant-based oil parameters using Response Surface Methodology (RSM). A three-level three-factor Box-Behnken design (BBD) was used to investigate the interactions between the essential factors comprising load (45 kg to 90 kg), speed (60 rpm, 90 rpm and 360 rpm), and concentration (0ml, 5ml and 10ml) of bio-lubricants to accomplish the indicated prospect of using polymer for cartilage replacement. Canola oil, castor oil, and sunflower seed oil are considered vegetable oils, whereas Hyaluronic Acid (HA) is the friction modifier. The parameters are used to create a Box-Behnken design for predicting lubricant anti-wear qualities stated in terms of coefficient of friction, wear rate and frictional force as determined by the pin-on-disk experimental procedure. As a result, the optimization using RSM successfully interpreted the experimental data, according to the analysis of variance, with coefficients of determination of R2 = 0.91 and adjusted R2 = 0.77. The Coefficient of Friction (CoF) and wear rate were investigated following tribological testing. Castor oil had a lower coefficient of friction than canola and sunflower seed oil, according to the findings. In terms of friction reduction, castor oil surpasses canola and sunflower seed oil.

INVESTIGATION OF THE RHEOLOGICAL, NUTRITIONAL, AND SENSORY PROPERTIES OF THE GLUTEN-FREE BREAD PRODUCED FROM RICE, BEAN, AND CHICKPEA FLOUR

In recent years, the demand for gluten-free products has been constantly increasing due to the increase in the number of people suffering from celiac disease. Bread is the main product consumed in our country; therefore, gluten-free bread for celiac patients on the market is mostly of poor quality and low nutritional value, while gluten-free flour has a very high price. The purpose of this work was to investigate the rheological properties of dough, the nutritional value of bread, as well as the sensory properties of gluten-free bread produced from mixtures of raw materials such as rice, chickpea, and bean flour, corn starch, and egg powder. The results showed that the gluten-free bread had different rheological properties, and only mixes M5 and M6 had similar rheological properties, such as dough development, stability, and degree of softening, to the control bread M1. Gluten-free bread from mixtures M2 and M3 had higher contents of protein, fat, cellulose, and minerals, while bread from mixtures M5 and M6 had medium contents. Bread M2 and M3 had acidity levels above the allowed limits which made them unusable or had a short shelf life. Sensory analyses showed that bread M5 had similar sensory properties to the control bread M1 and fell into the same quality category; also, bread from the M5 mixture has a much better taste and aroma. For consumption, we recommend using gluten-free bread from the M5 mixture, which in its composition has 80% rice flour, 7.5% chickpea flour, 5% bean flour, and 7.5% egg powder.

An Investigation of AI-Enabled Comprehensive Survey of Phishing Attacks detection techniques

Phishing is an online criminal activity which traps regular online customers to reveal their sensitive information into fake destinations by disguising themselves as original website. Because of the short life expectancy of phishing sites and the fast progression of phishing systems the current anti- phishing solutions are either unfit to manage the rising changes or in fit for assuming a successful part against this attack.Natural language handling and machine learning – these are the centre usage of the multi-stage approach proposed to identify phishing attacks and to perceive the substance/association that has been misused by the attackers to execute the phishing attacks. In this technique the primary stage is the revelation of named elements (names of areas, individuals and associations) and afterward the disclosure of concealed topics and for this the strategies that backings both phishing and non-phishing information therefore, Conditional Random Field (CRF) and Latent Dirichlet Allocation (LDA) is utilized. Next stage is the AdaBoost arrange where the named elements and the concealed subjects are dealt as features and the messages are ordered into phishing or non-phishing. proposed techniques are planned to alleviate the effect of phishing attack. The principal strategy proposes is to identify Wi-Fi phishing utilizing Association Rule mining. A Wi-Fi hotspot which associates such hand held gadgets has turned into a transitory archive of delicate data, in this way giving an open door for programmers to gather money related gain .Consequently data security arrangements tending to Wi-Fi hotspots (Wi-Fi phishing attack) has turned into the need of great importance. The conceivable approaches to attack the security of Wi-Fi hotspots and counter-attack methodologies have been tended to in this strategy. Key works: Phishing, LDA,AI

Internal Disorders of Mango Fruit and Their Management-Physiology, Biochemistry, and Role of Mineral Nutrients.

Mango (Mangifera indica L.) is a popular fruit grown in tropical and subtropical regions. Mango has a distinctive aroma, flavour, and nutritional properties. Annual global mango production is >50 million tonnes. Major producers of mango include India, Bangladesh, China, Mexico, Pakistan, Indonesia, Brazil, Thailand, and the Philippines, and it is shipped worldwide. Harvested mango fruit are highly perishable, with a short shelf life. Physiological disorders are among the major factors limiting their postharvest quality and shelf life, including when fruit need phytosanitary treatments, such as hot water treatment, vapour heat treatment, and irradiation. This review focuses on problematic physiological disorders of mango flesh, including physiology and biochemistry. It considers factors contributing to the development and/or exacerbation of internal disorders. Improved production practices, including pruning, nutrient application, and irrigation, along with monitoring and managing environmental conditions (viz., temperature, humidity, and vapour pressure deficit), can potentially maintain fruit robustness to better tolerate otherwise stressful postharvest operations. As demand for mangoes on international markets is compromised by internal quality, robust fruit is crucial to maintaining existing and gaining new domestic and export consumer markets. Considering mango quality, a dynamic system, a more holistic approach encompassing pre-, at-, and post-harvest conditions as a continuum is needed to determine fruit predisposition and subsequent management of internal disorders.

Effective measures to control eimeriosis in poultry in the Republic of Dagestan

The most common disease of young poultry in commercial farms of the Russian Caspian region is eimeriosis. In most cases, after convalescence from coccidiosis caused by one of Eimeria species poultry remains susceptible to other species. This parasite has a very short life cycle and immense reproductive capacity that is why it can cause large-scale outbreaks of the disease in commercial poultry houses. To control avian eimeriosis, various coccidiostats are used in combination with probiotics and vitamins. Frequent and long-term use of the same drugs against this infection can potentially result in the emergence of resistant Eimeria populations. This suggests that this coccidiosis control requires rotation of eimeriocidal drugs. Studies on eimeriosis prevalence were performed in the laboratory of the Caspian Regional Research Veterinary Institute and in different poultry farms of the Republic of Dagestan. Swabs of the floor, litter, equipment, droppings, feedstuffs, cecum smears from dead poultry were used for testing. High infection rate with eimerias was established in floor-housed poultry in the plain and piedmont zones of the Republic (Khasavyurtovsky and Karabudakhkentsky raions), where the infection rates were 81.6 and 82.4%, respectively. In batter-cage system poultry farms of the mountain and mountain valley zones (Khunzakhsky and Gergebilsky raions) the infection rates were significantly lower – 61.2 and 58.0%, respectively. The comparative efficacy study of two eimeriocidal drugs showed that “Robenidine”, used daily from the first day of life during the entire rearing period at a dose of 33 g per 1 ton of feedstuffs controls coccidiosis in poultry. At the same time, the survival rate of the experimental young poultry during the observation period was 98.0% compared with “Sarucoxum 12%” group (96.7%).

Educational Poets of Uzbekistan and Indonesia: Usman Nasir and Chairil Anwar.

The subjects of our research are Usman Nasir (1913-1944) and Chairil Anwar (1922-1949). Comparative study of Uzbek poet Usman Nasir and Indonesian poet Chairil Anwar allows us to uncover hidden truths of both history and literature. Comparative study can explore the remarkable similarities and features much more deeply than studying just one thing. In addition, not only will the literature of the two countries be renewed, but literary cooperation between the two countries will be strengthened. During the short lives of these two poets — about thirty years apart — they left behind original works that contributed to the rich literary heritage of their country. They were able to capture the suffering of their people in writing. They were also punished for using such bitter words. However, they continued to create until the very end of their lives.

Sabellaria wilsoni Lana and Gruet, 1989 on the Brazilian Amazon coast: Fast growth with high mortality, production, and turnover rate

Given the enormous ecological importance of sabellariid (Annelida: Sabellariidae) reefs for marine environments, data on the life history traits of these reef builders are essential for understanding ecosystem function. We assessed the functional parameters of a population of Sabellaria wilsoni (Annelida: Sabellariidae) on the Brazilian Amazon coast, where this polychaete builds extensive reef systems. The species had fast growth, a relatively short life span (1.30 years), and a high mortality rate (3.87 year−1). The mean annual biomass (58 g AFDM m−2) and production (233 g AFDM m−2 year−1) were very high and resulted in a high production-to-biomass ratio (P/B ratio: 4.02 year−1). The months with the highest productivity were those with the greatest reef coverage and density of worms, which coincided with the periods of reef growth. The high P/B ratio indicates the rapid replacement of biomass in this tropical population. These data support the hypothesis that S. wilsoni is an r-strategist, which is favored by the elevated temperatures and abundance of feeding resources and grains (for tube building) found on the Amazon coast. The present study is the first to produce systematic data on the population dynamics and production of a sabellariid species in the Western Atlantic Ocean. These data provide a baseline for understanding how shallow-water trophic webs function, as well as for monitoring these coastal habitats in the tropics.

Low voltage electrostatic field combined with ice-temperature to improve the quality of litchi during storage

Litchi is popular among consumers due to its delicious taste, however, the extremely short shelf life limits its commercial value. Few studies have been conducted to develop new technologies to extend the shelf life of litchi. Therefore, this study applied a novel technique (low voltage electrostatic field, LVEF) combined with ice-temperature (0 °C) treatment on litchi and evaluated its quality characteristics during storage. The results demonstrated that low voltage electrostatic field with ice-temperature storage (LVEF-IT) decreased malondialdehyde content, relative electrolyte leakage, and polyphenol oxidase and peroxidase activities, successfully delayed the start of peel browning. Additionally, it preserved the fruit pulp’s excellent quality, which included high concentrations of soluble solids, ascorbic acid, and soluble protein. These findings imply that LVEF-IT treatment may be a novel technique for extending the storage time of litchi.

Effects of Cultivar Factors on Fermentation Characteristics and Volatile Organic Components of Strawberry Wine.

Strawberry wine production is a considerable approach to solve the problem of the Chinese concentrated harvesting period and the short shelf life of strawberries, but the appropriative strawberry cultivars for fermentation are still undecided. In this study, the strawberry juice and wines of four typical strawberry cultivars named Akihime (ZJ), Sweet Charlie (TCL), Snow White (BX), and Tongzhougongzhu (TZ) were thoroughly characterized for their physicochemical indicators, bioactive compounds, and volatile organic components (VOCs) to determine the optimal strawberry cultivars for winemaking. The results showed that there were significant differences in the total sugar content, pH, total acid, and other physicochemical indexes in the strawberry juice of different cultivars, which further affected the physicochemical indexes of fermented strawberry wine. Moreover, the content of polyphenols, total flavonoids, vitamin C, and color varied among the four strawberry cultivars. A total of 42 VOCs were detected in the strawberry juice and wines using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS), and 3-methyl-1-butanol, linalool, trans-2-pinanol, hexanoic acid, and hexanoic acid ethyl ester were the differential VOCs to identify the strawberry wine samples of different cultivars. Overall, strawberry cultivar ZJ had a relatively high VOC and bioactive compound content, indicating that it is the most suitable cultivar for strawberry wine fermentation. In addition to determining the relatively superior fermentation characteristics of cultivar ZJ, the results may provide a theoretical basis for the raw material quality control and quality improvement of strawberry wine.

Dynamic pricing for perishable goods: A data-driven digital transformation approach

In supermarkets, rapid pricing adjustments are crucial due to the short shelf life of products. Adopting a multi-case study approach, this study examines the application of dynamic pricing strategies for perishable goods through the lens of a data-driven digital transformation (DD-DT) approach. We introduce a three-phase model of DD-DT for dynamic pricing: initiation, facilitation, and strategic adaptation. In the initiation phase, we identify essential frameworks for robust data collection and analytical processes, which form the backbone of informed pricing decisions. During the facilitation phase, the study integrates sophisticated algorithms and real-time analytics to process and interpret the collected data, facilitating its seamless integration into pricing strategies. The strategic adaptation phase is critical as it focuses on the ongoing refinement and enhancement of pricing strategies, enabling supermarkets to adapt swiftly to market fluctuations and consumer behaviour changes. By presenting a comprehensive DD-DT framework, this research significantly augments the existing literature on dynamic pricing and offers actionable insights for practitioners seeking to optimize pricing strategies in a digitally transforming marketplace.

3D self-supporting core-shell silicon-carbon nanofibers-based host enables confined Li+ deposition for lithium metal battery

Three-dimensional (3D) porous hosts play pivotal roles in realizing dendrite-free lithium metal anodes (LMAs) owing to their high specific area. However, uneven local electric field and lack of lithiophilic sites on the reactive interface cause nonuniform lithium ion (Li+) deposition, leading to Li dendrite growth and parasitic reactions. These issues will inevitably incur short cycling life and severe safety hazards of lithium metal batteries. Herein, a 3D self-supporting host composed of hollow carbon nanofibers incorporated with silicon (Si) nanoparticles inside (Si-HCF) is constructed as Li metal host by a scalable coaxial electrospinning technique. During the first cycle, the Si nanoparticles is alloyed with Li+ to form lithiophilic Li-Si alloy, contributing to deliver a low nucleation overpotential and homogeneous surface potential confirmed by Kelvin probe force microscopy, finally guiding Li+ flux homogeneously throughout the fibrous mat. As expected, the Li metal can percolate through Si-HCF host reversibly without uncontrollable Li dendrites and the Si-HCF exhibits a stable cycle life of Li plating/stripping over 1400 h. Additionally, the full cell combined with LiFePO4 cathodes presents steady cycling over 400 cycles with a high average coulombic efficiency of 99.4 %. This work provides new insights for the synthesis of 3D hosts with controllable nucleation sites to homogenize Li+ deposition in LMAs.

Time preference shifts in medical decision-making after serious illness

This study explores the impact of serious illnesses, such as cancer, on patients' time preferences in medical decision-making. Specifically, we assess how patients value extending their lifespan by one year under varying survival prognoses through three experimental studies. The findings reveal that patients exhibit a higher Subjective Discount Rates (SDR) in their medical decisions after a serious illness diagnosis. Notably, this difference in individual health also affects the time preferences of their family members. Additionally, the subjective contextual setting of the illness can also increase an individual's SDR levels. The research highlights a tendency for patients and families facing a potential short life expectancy to focus more on immediate concerns, leading to potentially shortsighted and irrational medical choices. This behavior often results in regret during the end-of-life stage. These insights are vital for healthcare professionals in optimizing treatment plans and for policymakers in understanding patient behaviors more comprehensively. The study emphasizes the need for considering psychological and behavioral changes in patients grappling with severe health challenges.

Composite Coatings of Gellan Gum and Inulin with Lactobacillus casei: Enhancing the Post-Harvest Quality of Guava

Guava is a highly sought-after tropical fruit in the market due to its high content of vitamins, minerals, antioxidants, and other phenolic compounds. However, due to its climacteric nature, it has a short post-harvest shelf life. The aim of this study was to develop coatings based on gellan gum (GG) and inulin (IN) incorporating Lactobacillus casei, which were tested for their potential ability to extend the post-harvest shelf life of whole guava fruit. The coatings were prepared using the following formulations: 0.5 GG/1.0 IN (w/v), 0.8 GG/5.0 IN (w/v), 0.5 GG/1.0 IN(w/v), and 0.8 GG/5.0 IN (w/v). The coated and uncoated (control) fruits were stored at 25 °C for 12 days, and various quality attributes were evaluated (including respiration rate, soluble solids, titratable acidity, weight loss, total phenol content, and color). The results indicated that the application of the coatings reduced weight loss, color change, and respiration rate in the fruits. However, the 0.8 GG/5.0 IN (w/v) formulation provided the best maintenance of post-harvest quality for the fruit evaluated. The coatings with a higher inulin content showed the highest growth of L. casei, which could enhance the antimicrobial effect of the coating. Therefore, the combined application of L. casei and inulin in coatings based on gellan gum can be considered an effective treatment to extend the shelf life and preserve the quality of guava fruits.

Lithium fluoride additive helps construct stable electrode-electrolyte interfaces to boost the performance of Mg-S batteries

Most magnesium electrolytes react with the Mg anode surface to form a non-favorable passive layer that inhibits shuttling of the Mg ions and results in the short cycle life of the Mg cells. In this work, we probe LiF as an effective electrolyte additive to address the challenges of electrolyte degradation, Mg anode passivation, and polysulfide shuttle. The key to our strategy lies in modifying the halogen-free electrolytes (HFE) electrolyte-based Mg(NO3)2·6H2O salt dissolved in acetonitrile (ACN) and tetra ethylene glycol dimethyl ether (G4) with LiF additives, which play a crucial role in engineering stable electrolyte interfaces. These additives alter the chemical composition of the solid electrolyte interface (SEI) layer on the surface of the Mg anode, enabling low overpotential of Mg2+ extraction/insertion, improving the Mg2+ transference, and enhancing the electrochemical performance of the electrolyte. The Mg-S cell with modified electrolyte delivers a high discharge capacity of over 400 mAh g−1 for >10 cycles, compared with the short cycle life in the case of using a blank electrolyte. This remarkable performance underscores the potential of our findings in advancing magnesium sulfur battery technology.

Influence of Soursop Fruit Tea Concentration on the Fermentation and Quality Attributes of Water Kefir Beverages

Soursop is a seasonal and highly perishable fruit with a short shelf life after harvest. Therefore, fermentation with water kefir could be a great combination to develop a novel probiotic and dairy-free alternative beverage with distinctive flavors all year round. Therefore, this study aimed to formulate and characterize fermented beverages utilizing soursop fruit tea and water kefir microorganisms. An evaluation of physical parameters, antioxidant capacity, microbiological profiles, and sensory attributes was conducted after the fermentation process of the soursop water kefir. The result indicated that pH and Brix values remained in the acceptance range for consumption. According to microbiological results, all water kefir samples provided a high number of lactic acid bacteria and yeasts, although no significant differences were observed in microbial counts. Furthermore, the organoleptic acceptability of the fruit water kefirs was encouraging, particularly at a 1% initial soursop fruit tea concentration, with favorable ratings for taste and overall acceptability.