Shortgrass Prairie Research Articles

Interpreting floodplain heterogeneity: Using field data to understand unsupervised floodplain classifications

The dynamic environment of natural river floodplains creates spatial heterogeneity that influences floodplain functions. Diverse human activities have homogenized natural floodplains and reduced their functions across many river networks in the temperate latitudes. Consequently, quantification of floodplain heterogeneity is needed to understand patterns of spatial heterogeneity on diverse floodplains and to inform floodplain restoration. We use a novel approach of spatially connecting field and remotely sensed data in order to interpret the output of, and build upon, a previous unsupervised classification workflow. We apply the method to three rivers in the US Pacific Northwest and the Altamaha River in the southeastern US and compare our results to a previous study. We find that field classifications, relative topography, and NDVI are useful for interpreting results from the unsupervised classification workflow. The interpretations are visually interesting, but we propose that it is the heterogeneity within the groups that is vital to floodplain functioning. Natural floodplains in the Pacific Northwest and coastal Southeast have moderate to high evenness, moderate to high intermixing, and moderate aggregation; and aggregation and evenness similar to rivers in Colorado and Oklahoma, USA, but lower intermixing. We attribute lower intermixing at the Altamaha River to slower rates of lateral channel migration, and lower intermixing at the Hoh River to the different hydrologic and sediment regimes and less stable braided planform. The results show that the larger rivers in this study (Altamaha, Hoh, and Sol Duc Rivers) have spatial heterogeneity similar to beaver-modified and shortgrass prairie rivers in Colorado, whereas the more inland and smaller river (Lookout Creek) has spatial heterogeneity similar to the tallgrass prairie site (Sand Creek). From the results of an ad hoc sensitivity analysis, we suggest using the highest spatial resolution topographic data available, using aerial imagery/mosaics from the same sensor, and removing largest patch index from the suite of comparable indices. The metrics reveal similarities and differences between rivers in the United States, and indicate that discernable trends may arise from a meta study comparing heterogeneity from more rivers across the country.

Temporal changes in genetic diversity reveal small-scale invasion dynamics of the eastern redcedar (Juniperus virginiana var. virginiana) in the Lakeside Daisy State Nature Preserve in Ohio

AbstractEastern redcedar (Juniperus virginiana L. var. virginiana; hereafter ERC) is a native species currently invading open areas and grasslands outside of its original range in the United States. We studied ERC’s invasion patterns in the Lakeside Daisy State Nature Preserve (LDSNP), a short grass prairie located on the Marblehead Peninsula in Ohio, examining the changes in the genetic diversity and structure of the encroaching population. We investigated the relative importance of long-distance dispersal versus diffusion in the invasion of this short grass prairie by ERC. We use eight microsatellite marker loci to infer gene flow from external sources versus within-population recruitment. We found that the older trees in this preserve were less than 50 yr old, indicating that the population was established between 1970 and 1980. When we grouped trees into five age categories of 10-yr increments, we found that the allelic diversity, as indicated by the average number of alleles per locus, increased as the age of the trees decreased. We also found that not all loci were in Hardy-Weinberg equilibrium, probably due to the arrival of new variants in the preserve. Moreover, heterozygosity remained high, with an excess of heterozygotes in all age groups (F = −0.163 ± 0.046). Principal coordinate analysis showed two distinct groups of trees in the LDSNP. Analysis of the cryptic population structure of the ERC trees using STRUCTURE revealed four ancestral clusters in the ERC population. All ancestral clusters are present in all age groups, suggesting that all trees sampled are derived from an admixed population. Furthermore, the high observed heterozygosity and lack of inbreeding in this dioecious species maintained all ancestral clusters over time. Overall, our findings indicate that ERC encroachment of the LDSNP results from multiple and reiterated gene flow events from the edge of the range through animal-mediated seed dispersal.

Boys and Oil: Growing Up Gay in a Fractured Land by Taylor Brorby

Reviewed by: Boys and Oil: Growing Up Gay in a Fractured Land by Taylor Brorby O. Alan Weltzien and emeritus Taylor Brorby, Boys and Oil: Growing Up Gay in a Fractured Land. New York: Liveright Publishing. 335 pp. Hardcover, $27.95. Montana novelist Thomas Savage, a gay man heterosexually married, threw his overtly gay novel manuscript into the Atlantic (ca. 1964) when his literary agent advised him it couldn’t be published let alone widely read. As we all know by now, his character, repressed gay Phil Burbank (The Power of the Dog), who contains strands of Savage, meets a painful death as he tries to come out again. In his friend Annie Proulx’s best-known short story, “Broke-back Mountain,” Ennis angrily asks his lover, “This happen a other people? What the hell do they do?” and Jack responds, “It don’t happen in Wyoming and if it does I don’t know what they do, maybe go to Denver.” As you might remember, a year after this short story was published, Matthew Shepard, a Wyomingite, didn’t make it that far. Even now, how much safe space exists for sexual minorities in the rural West away from the coastal region? That question arguably represents the central prompt behind Taylor Brorby’s beautifully written memoir. He reminds us that it’s unsafe, outside some urban precincts, for a man to hold another man in public. Brorby’s story shows him inexorably torn between his western North Dakota home and exile. He insistently and lyrically paints his native topography in the country’s least visited state. He’s a poet of a landscape few of us know let alone cherish, and his love runs deep. At the same time, as his title and subtitle clarify, this memoir tells an all-too-familiar story of environmental degradation and destruction, of callous extractive industries that strip-mine surfaces, gouge the depths, and leave scarred ground that’s the short-grass prairie equivalent of Pacific Northwest clear-cutting. He knows the Bakken oil patch and the Dakota Access Pipeline personally and painfully, as his anthology Fracture (2016) attests. He records his Iowa arrest for protesting the Dakota Access Pipeline (260–61), about which he’s written elsewhere. Brorby knows fracking means fucking the ground beneath: yet another example of Rob Nixon’s slow violence. North Dakota means [End Page 80] a horizontal place defined by a five-hundred-foot smokestack and a dragline and endless derricks; it is a “story of self-destruction. Everything leaves North Dakota full and comes back empty. The only way I’ve understood my home is by getting out, escaping its crushing weight, watching the destruction, now from the outside” (7). Our endless pursuit of oil leaves only wreckage in its wake—no new news—and the kind of boys (and girls) drawn to oil patches pose direct threats to the Native boy who, by adolescence, in familiar fashion, discovers himself a sexual minority subject to insult, ostracism, and worse. Brorby begins, “Center [North Dakota] is a place where people only end up” (5). His memoir makes a strong case for escape. He’s escaped to Bismarck by high school and St. Olaf’s College then the Twin Cities as he struggles through identity formation. It’s the old American story of getting the hell out of Dodge particularly if one doesn’t conform to local majority preferences, including heterosexuality. Brorby lives through extensive pain and seasons of tears as he negotiates his emerging self between his roots, his family and place, and new, more tolerant and cosmopolitan geographies. What a courageous story of redefining what and where one belongs. The most painful part of Brorby’s story concerns his parents who provided him and his older sister a range of creature comfort but who turned their backs when he came out to them. In such a memoir, coming out, unsurprisingly, forms a main theme and Brorby sketches several scenes in which his older sister, his grandfathers, and ultimately his two older nephews, affirm him and their unqualified love. Those embraces make his parents’ silent hostility all the more excruciating (they’ve not spoken in years). The...

Grassland ecosystem type drives AM fungal diversity and functional guild distribution in North American grasslands.

Nutrient exchange forms the basis of the ancient symbiotic relationship that occurs between most land plants and arbuscular mycorrhizal (AM) fungi. Plants provide carbon (C) to AM fungi and fungi provide the plant with nutrients such as nitrogen (N) and phosphorous (P). Nutrient addition can alter this symbiotic coupling in key ways, such as reducing AM fungal root colonization and changing the AM fungal community composition. However, environmental parameters that differentiate ecosystems and drive plant distribution patterns (e.g., pH, moisture), are also known to impact AM fungal communities. Identifying the relative contribution of environmental factors impacting AM fungal distribution patterns is important for predicting biogeochemical cycling patterns and plant-microbe relationships across ecosystems. To evaluate the relative impacts of local environmental conditions and long-term nutrient addition on AM fungal abundance and composition across grasslands, we studied experimental plots amended for 10 years with N, P, or N and P fertilizer in different grassland ecosystem types, including tallgrass prairie, montane, shortgrass prairie, and desert grasslands. Contrary to our hypothesis, we found ecosystem type, not nutrient treatment, was the main driver of AM fungal root colonization, diversity, and community composition, even when accounting for site-specific nutrient limitations. We identified several important environmental drivers of grassland ecosystem AM fungal distribution patterns, including aridity, mean annual temperature, root moisture, and soil pH. This work provides empirical evidence for niche partitioning strategies of AM fungal functional guilds and emphasizes the importance of long-term, large scale research projects to provide ecologically relevant context to nutrient addition studies.

Next-generation technologies unlock new possibilities to track rangeland productivity and quantify multi-scale conservation outcomes

Historically, relying on plot-level inventories impeded our ability to quantify large-scale change in plant biomass, a key indicator of conservation practice outcomes in rangeland systems. Recent technological advances enable assessment at scales appropriate to inform management by providing spatially comprehensive estimates of productivity that are partitioned by plant functional group across all contiguous US rangelands. We partnered with the Sage Grouse and Lesser Prairie-Chicken Initiatives and the Nebraska Natural Legacy Project to demonstrate the ability of these new datasets to quantify multi-scale changes and heterogeneity in plant biomass following mechanical tree removal, prescribed fire, and prescribed grazing. In Oregon's sagebrush steppe, for example, juniper tree removal resulted in a 21% increase in one pasture's productivity and an 18% decline in another. In Nebraska's Loess Canyons, perennial grass productivity initially declined 80% at sites invaded by trees that were prescriptively burned, but then fully recovered post-fire, representing a 492% increase from nadir. In Kansas' Shortgrass Prairie, plant biomass increased 4-fold (966,809 kg/ha) in pastures that were prescriptively grazed, with gains highly dependent upon precipitation as evidenced by sensitivity of remotely sensed estimates (SD ± 951,308 kg/ha). Our results emphasize that next-generation remote sensing datasets empower land managers to move beyond simplistic control versus treatment study designs to explore nuances in plant biomass in unprecedented ways. The products of new remote sensing technologies also accelerate adaptive management and help communicate wildlife and livestock forage benefits from management to diverse stakeholders.

Reference genome of the California glossy snake, Arizona elegans occidentalis: A declining California Species of Special Concern.

The glossy snake (Arizona elegans) is a polytypic species broadly distributed across southwestern North America. The species occupies habitats ranging from California's coastal chaparral to the shortgrass prairies of Texas and southeastern Nebraska, to the extensive arid scrublands of central México. Three subspecies are currently recognized in California, one of which is afforded state-level protection based on the extensive loss and modification of its preferred alluvial coastal scrub and inland desert habitat. We report the first genome assembly of A. elegans occidentalis as part of the California Conservation Genomics Project (CCGP). Consistent with the reference genome strategy of the CCGP, we used Pacific Biosciences HiFi long reads and Hi-C chromatin-proximity sequencing technologies to produce a de novo assembled genome. The assembly comprises a total of 140 scaffolds spanning 1,842,602,218 base pairs, has a contig NG50 of 61 Mb, a scaffold NG50 of 136 Mb, and a BUSCO complete score of 95.9%, and is one of the most complete snake genome assemblies. The A. e. occidentalis genome will be a key tool for understanding the genomic diversity and the basis of adaptations within this species and close relatives within the hyperdiverse snake family Colubridae.

Sustainable reclamation practices for a large surface coal mine in shortgrass prairie, semiarid environment (Wyoming, USA): case study

Sustainable reclamation practices for large surface coal mines in USA semiarid environment contribute to the quality of the environmental on a long term basis where environmental resources are protected for future generation. Land, after reclamation, must be suitable for the previous use of greatest economic or social values to the community area. In the semiarid climate of USA, non-developed land is mainly utilized for crops, grazing, and wildlife. Completion of various stages of the reclamation processes includes verification and approval of reclamation criteria and performance standards created by state agencies. The sustainable reclamation practices were investigated at the USA’s largest surface coal mine of the semiarid environment in Wyoming. These practices include building post-mining topography to the approximate original contour and reestablish a stable hydrologic system to drain surface water. All available spoil material is backfilled and graded to achieve the post-mining topography which closely resembles the pre-mining topography. No overburden material or other coal waste material is left in stockpiles at the mine. Detailed planning until the end of mining, the knowledge of available volumes of suitable backfill material and soil is necessary for sustainable management practices. Diverse and permanent vegetation capable of stabilizing soil surfaces and capable of self-regeneration is established. Sustainable management of the reclamation effort is achieved by enforcement processes developed by the state and federal agencies. Monthly inspections of mining and reclamation operations and reviews of annual reports submitted by the operator help determine if the reclamation processes are occurring according to the permit plan.

Land enrolled in the Conservation Reserve Program supports roosting ecology of the lesser prairie-chicken

All animals must select sites to rest and may spend a large portion of their lives doing so. Despite the importance of this period in their daily activity budget, we lack information about rest/roost ecology for most animals, including the imperiled lesser prairie-chicken ( Tympanuchus pallidicinctus ; hereafter “LEPC”). Therefore, we sought to identify how landcover, anthropogenic features, and human policy (i.e., presence of the Conservation Reserve Program [CRP]) influenced roost site selection and movement patterns of the LEPC. From March to May 2013–2015, we captured and fitted GPS transmitters to 106 LEPCs ( n = 72 males; n = 34 females) within Beaver County, Oklahoma and recorded two nocturnal locations per 24-hour period, annually. We used discrete choice models and generalized linear mixed effects models to determine how vegetation cover, CRP patches, and anthropogenic features influenced roost site selection and movements to roosting sites, respectively. We found that roost sites were closer to CRP, leks, and croplands than would be expected at random. Conversely, roost sites were located farther away from shortgrass prairie, roads, and transmission lines than expected. The probability of a LEPC roosting in a location increased by 15% and 4.5% for every 36-meter decrease in distance to a CRP patch and distance to their lek of capture, respectively. Similarly, individuals roosting in CRP patches would travel shorter distances to get to roost sites if their last diurnal location was near CRP. Bird’s movements to roosts in CRP were 1.4 times shorter for every meter decrease in the distance of their last diurnal location to a CRP patch, indicating that individuals modify their movement based on CRP presence. Our results indicate that CRP is influential to roosting ecology of the LEPC within this region and may be meeting critical cover requirements.

Breeding Season Space Use by Lesser Prairie-Chickens (Tympanuchus Pallidicinctus) Varies Among Ecoregions and Breeding Stages

Large-scale declines of grassland ecosystems in the conterminous United States since European settlement have led to substantial loss and fragmentation of lesser prairie-chicken (Tympanuchus pallidicinctus) habitat and decreased their occupied range and population numbers by ∼85%. Breeding season space use is an important component of lesser prairie-chicken conservation, because it could affect both local carrying capacity and population dynamics. Previous estimates of breeding season space use are largely limited to one of the four currently occupied ecoregions, but potential extrinsic drivers of breeding space use, such as landscape fragmentation, vegetation structure and composition, and density of anthropogenic structures, can show large spatial variation. Moreover, habitat needs vary greatly among the lekking/prelaying, nesting, brood-rearing, and postbreeding stages of the breeding season, but space use by female lesser prairie-chickens during these stages remain relatively unclear. We tested whether home range area and daily displacement (the net distance between the first and last location of each day) of female lesser prairie-chickens varied among ecoregions and breeding stages at four study sites in Kansas and Colorado, U.S.A., representing three of the four currently occupied ecoregions. We equipped females with very-high-frequency (VHF) or Global Positioning System (GPS) transmitters, and estimated home range area with kernel density estimators or biased random bridge models, respectively. Across all ecoregions, breeding season home range area averaged 190.4 ha (±19.1 ha se) for birds with VHF and 283.6 ha (±23.1 ha) for birds with GPS transmitters, whereas daily displacement averaged 374.8 m (±14.3 m). Average home range area and daily displacement of bird with GPS transmitters were greater in the Short-Grass Prairie/ Conservation Reserve Program Mosaic and Sand Sagebrush Prairie Ecoregions compared to sites in the Mixed-Grass Prairie Ecoregion. Home range area and daily displacement were greatest during lekking/prelaying and smallest during the brood-rearing stage, when female movements were restricted by mobility of chicks. Ecoregion- and breeding stage-specific estimates of space use by lesser prairie-chickens will help managers determine the spatial configuration of breeding stage-specific habitat on the landscape. Furthermore, ecoregion- and breeding stage-specific estimates are crucial when estimating the amount of breeding habitat needed for lesser prairie-chicken populations to persist.

Activity and Overlap Among Birds and Mammals Scavenging A Bison Carcass in Shortgrass Prairie

Bison serve as keystone species in prairie ecosystems of North America, yet few studies have evaluated the effects of bison carcasses on other animals. To determine which species forage on bison carcasses and whether such carcasses catalyze intraspecific and interspecific interactions, we used wildlife cameras to observe scavenger visits and activity over a 6-wk period following the death of an adult female bison from a conservation herd in northern Colorado. We captured more than 45 000 photos of avian and mammalian scavengers. Photos most often included coyote (51% of photos) and common raven (50%), followed by black-billed magpie (14%) and golden eagle (0.11%). Most photos (86%) captured individuals of the same species (intraspecific activity) visiting the carcass, while 14% had two or more different species (interspecific activity) at the carcass simultaneously. The species most often photographed together included the common raven and black-billed magpie (64%), followed by coyote and black-billed magpie (25%) and coyote and common raven (7%). Less than 1% of photos captured dominance behaviors among individuals. Our study demonstrates that bison carcasses have the potential to provide key food resources in the winter for shortgrass prairie animals. The role of bison carcasses in supporting grassland animal communities is likely to become increasingly important as efforts gain momentum to restore bison across their historic range in North America.

Mapping shifts in spatial synchrony in grassland birds to inform conservation planning.

Spatial synchrony, defined as the correlated fluctuations in abundance of spatially separated populations, can be caused by regional fluctuations in natural and anthropogenic environmental population drivers. Investigations into the geography of synchrony can provide useful insight to inform conservation planning efforts by revealing regions of common population drivers and metapopulation extinction vulnerability. We examined the geography of spatial synchrony and decadal changes in these patterns for grassland birds in the United States and Canada, which are experiencing widespread and persistent population declines. We used Bayesian hierarchical models and over 50 years of abundance data from the North American Breeding Bird Survey to generate population indices within a 2° latitude by 2° longitude grid. We computed and mapped mean local spatial synchrony for each cell (mean detrended correlation of the index among neighboring cells), along with associated uncertainty, for 19 species in 2, 26-year periods, 1968-1993 and 1994-2019. Grassland birds were predicted to increase in spatial synchrony where agricultural intensification, climate change, or interactions between the 2 increased. We found no evidence of an overall increase in synchrony among grassland bird species. However, based on the geography of these changes, there was considerable spatial heterogeneity within species. Averaging across species, we identified clusters of increasing spatial synchrony in the Prairie Pothole and Shortgrass Prairie regions and a region of decreasing spatial synchrony in the eastern United States. Our approach has the potential to inform continental-scale conservation planning by adding an additional layer of relevant information to species status assessments and spatial prioritization of policy and management actions. Our work adds to a growing literature suggesting that global change may result in shifting patterns of spatial synchrony in population dynamics across taxa with broad implications for biodiversity conservation.

Livestock grazing is associated with seasonal reduction in pollinator biodiversity and functional dispersion but cheatgrass invasion is not: Variation in bee assemblages in a multi-use shortgrass prairie.

Livestock grazing and non-native plant species affect rangeland habitats globally. These factors may have important effects on ecosystem services including pollination, yet, interactions between pollinators, grazing, and invasive plants are poorly understood. To address this, we tested the hypothesis that cattle grazing and site colonization by cheatgrass (Bromus tectorum) impact bee foraging and nesting habitats, and the biodiversity of wild bee communities, in a shortgrass prairie system. Bee nesting habitats (litter and wood cover) were marginally improved in non-grazed sites with low cheatgrass cover, though foraging habitat (floral cover and richness, bare soil) did not differ among cattle-grazed sites or non-grazed sites with low or high cheatgrass cover. However, floral cover was a good predictor of bee abundance and functional dispersion. Mean bee abundance, richness, diversity and functional diversity were significantly lower in cattle-grazed habitats than in non-grazed habitats. Differences in bee diversity among habitats were pronounced early in the growing season (May) but by late-season (August) these differences eroded as Melissodes spp. and Bombus spp. became more abundant at study sites. Fourth-corner analysis revealed that sites with high floral cover tended to support large, social, polylectic bees; sites with high grass cover tended to support oligolectic solitary bees. Both cattle-grazed sites and sites with high cheatgrass cover were associated with lower abundances of above-ground nesting bees but higher abundance of below-ground nesters than non-grazed sites with low cheatgrass cover. We conclude that high cheatgrass cover is not associated with reduced bee biodiversity or abundance, but cattle grazing was negatively associated with bee abundances and altered species composition. Although floral cover is an important predictor of bee assemblages, this was not impacted by cattle grazing and our study suggests that cattle likely impact bee communities through effects other than those mediated by forbs, including soil disturbance or nest destruction. Efforts aimed at pollinator conservation in prairie habitats should focus on managing cattle impacts early in the growing season to benefit sensitive bee species.

23 Current progress in the Agricultural Research Service Beef Grand Challenge: A large-scale genetics by environment by management evaluation project

Abstract The Agricultural Research Service (ARS) Beef Grand Challenge is a cooperative, multidisciplinary effort evaluating differences in performance of genetic lines across production environments representative of different geographical regions. Weaned spring-born calves (n = 120 per location), representing natural service matings to Angus, Hereford, Simmental, Charolais, or indicus-composite (Beefmaster or Brangus) bulls from the U.S. Meat Animal Research Center Germplasm Evaluation program in south central Nebraska, are sent to wheat pasture (central Oklahoma) and winter range (eastern Montana), and weaned fall-born calves (n=40 per location) are sent to summer grazing on shortgrass prairie (northeastern Colorado) and southern mixed-grass rangeland (western Oklahoma). All cattle are fed a finishing ration representative of the region that approximately matches energy content across locations. Each calving season has a matching counterpart of calves that remain in Nebraska on a calf-fed drylot program (receiving ration followed by longer finishing ration). Breeds and sires are represented equally, to the extent possible, at each location. To detect differences in breed effects at each location and average over yearly variation, the study is being replicated for 4 years. Weights, stress measures, carcass composition (marbling, yield grade, quality grade, etc.), steak tenderness and steak fatty acid composition are collected from each location. Additionally, rumen metagenomic composition, metagenomic samples, preharvest food safety samples, and feed intake measures are collected at some locations. Grazing impacts and supplemental range feeding are also being evaluated. One year of sampling has been completed, with numeric differences observed for marbling and tenderness as well as growth performance among locations. Statistical differences will be evaluated when replicate years are collected. The USDA is an equal opportunity provider and employer.

Plant traits related to precipitation sensitivity of species and communities in semiarid shortgrass prairie.

Understanding how plant communities respond to temporal patterns of precipitation in water-limited ecosystems is necessary to predict interannual variation and trends in ecosystem properties, including forage production, biogeochemical cycling, and biodiversity. In North American shortgrass prairie, we measured plant abundance, functional traits related to growth rate and drought tolerance, and aboveground net primary productivity to identify: species-level responsiveness to precipitation (precipitation sensitivity Sspp ) across functional groups; Sspp relationships to continuous plant traits; and whether continuous trait-Sspp relationships scaled to the community level. Across 32 plant species, we found strong bivariate relationships of both leaf dry matter content (LDMC) and leaf osmotic potential Ψosm with Sspp . Yet, LDMC and specific leaf area were retained in the lowest Akaike information criterion multiple regression model, explaining 59% of Sspp . Most relationships between continuous traits and Sspp scaled to the community level but were often contingent on the presence/absence of particular species and/or land management at a site. Thus, plant communities in shortgrass prairie may shift towards slower growing, more stress-resistant species in drought years and/or chronically drier climate. These findings highlight the importance of both leaf economic and drought tolerance traits in determining species and community responses to altered precipitation.

Impacts of holistic planned grazing with bison compared to continuous grazing with cattle in South Dakota shortgrass prairie

We assess holistic planned grazing outcomes in shortgrass prairie of the Northern Great Plains of North America. We compared key ecosystem functions on the ranch managed using adaptive multi-paddocks (AMP) grazing by bison with those on neighboring ranch paddocks managed using set stocked light continuous (LCG) and heavy continuous grazing (HCG) grazed by cattle. Sites on the neighboring ranches in each grazing category were paired for sampling by soil type and landscape position. In all paddocks, management practices had been constant for more than a decade. Positive results with AMP grazing include increased fine litter cover (P < 0.05), improved water infiltration (P < 0.06), two to three times the available forage biomass (P < 0.001), improved plant composition (P < 0.05), decrease in invasive plants (P < 0.05), and decrease in bare ground (P < 0.05). Higher infiltration occurred with AMP on soils having higher permeability but not on soils having a high clay content. Differences were greatest between AMP and HCG management with LCG being intermediate. Counterintuitively, herbaceous biomass in LCG was less than that of the more heavily stocked HCG (P < 0.05). This was due to decades of heavy continuous grazing resulting in HCG being dominated by invasive herbaceous plants of no forage value in contrast to LCG paddocks that had a greater proportion of palatable forages. The HCG paddocks were dominated by unpalatable invasive plants that were avoided by cattle. Soil carbon stocks increased under the AMP grazing but not on all soils. Total carbon stocks (TC), summing organic carbon and inorganic carbon, were not different between the AMP and LCG grazing strategies (P > 0.63) but both had higher TC values across all soils than HCG (P < 0.001). There were no differences in TC among grazing treatments on the different soils (P > 0.46) except on the Norrest silty clay loam soil that had the highest permeability. On this soil there were differences between AMP and HCG (P < 0.0001) and LCG and HCG (P < 0.0001). There were significantly lower TC levels at all soil depths with HCG than with AMP and LCG (P < 0.05). Using holistic planned grazing protocols with AMP grazing effectively limited overstocking and overgrazing by adjusting animal numbers to match available forage amounts and grazing for short periods followed by adequate recovery after grazing. This study indicated ecological improvements by AMP grazing on the 777 Bison Ranch compared to HCG pastures is contributing to improvements in this semi-arid short grass ecosystem.

The Carnivores of Quarry 3, Agate Fossil Beds National Monument

At the beginning of the twentieth century, in the remote upper reaches of the Niobrara River valley, paleontologists discovered an unbelievable trove of fossil bones eroding from two adjacent hillsides. Under these two “fossil hills” were buried entire skeletons of extinct animals that previously had been known only by fragmentary remains. Over the next two decades, leading museums of the day expended great effort exposing and then extracting whole portions of this bonebed for public exhibition and for research purposes. Many years later, scientists from the University of Nebraska–Lincoln reopened the old quarries to search for clues, long overlooked, that might explain why rhinoceroses, chalicotheres, and entelodonts had perished together millions of years ago at this particular place. In the process, they uncovered carnivore dens unrecognized by the first excavators. Today, the displays at Agate Fossil Beds National Monument lead visitors through the forensic evidence that discloses a sequence of events culminating in the demise of many hundreds of animals. The exhibits at the visitor center also include murals by artist Mark Marcuson and a diorama that uses full skeletal mounts, all illustrating the fascinating story of the Agate bonebed. In addition, the park is situated in a 3,000-acre preserve of shortgrass prairie and tells the story of rancher James Cook, who first noticed the bones in the 1880s, and his friendship with the scientists who came to excavate at Agate. Visitors can follow several trails to view the historic fossil sites, where wayside exhibits explain the paleontological investigations, and reconstruct the landscape of a dryland paleoriver valley and its animals, 22 million to 23 million years ago.

Ecological and social consequences of bison reintroduction in Colorado

Bison were instrumental in shaping North America's Great Plains. Interest in restoring this iconic species and their ecological role in grassland ecosystems is rapidly gaining momentum. To evaluate the potential for bison to enhance habitat quality for wildlife and catalyze public engagement in grassland conservation, we assessed both the ecological and social effects of a recent bison reintroduction (2015) to northern Colorado. Specifically, we explored the effect of bison reintroduction on: (a) bird density and habitat use, (b) mammal habitat use, (c) vegetation composition and structure, and (d) visitor connectedness, known as place attachment, to a shortgrass prairie. We predicted that bison reintroduction would reduce cover and height of some grasses and shrubs, which would increase density and habitat use for obligate shortgrass prairie birds, and increase habitat use for coyote and lagomorphs. In addition, we predicted that visitors would express stronger place attachment to this grassland once bison were reintroduced. To measure ecological and social responses, we surveyed birds, mammals, and plants; and conducted structured visitor surveys before and after bison reintroduction. We found few short‐term effects of bison on grassland bird density and habitat use, mammal habitat use, and vegetation composition and structure. However, we measured a significant increase in visitor place attachment to the grassland site 1 year after bison reintroduction. Our results suggest that a new bison reintroduction may have immediate positive benefits for connecting people to conservation, and that the ecological and social effects may unfold over different time scales. We recommend that future bison reintroduction efforts monitor ecological and social outcomes to advance reintroduction biology.

Parasitism of Black-Tailed Prairie Dogs by Linognathoides cynomyis (Phthiraptera: Polyplacidae).

The following study investigates louse parasitism of black-tailed prairie dogs (Cynomys ludovicianus (Ord, Rodentia: Sciuridae)) on 20 plots at 13 colonies in the short-grass prairie of New Mexico, USA, June-August, 2011-2012. Among 124 lice collected from 537 prairie dogs during 1,207 sampling events in which anesthetized animals were combed for ectoparasites, all of the lice were identified as Linognathoides cynomyis (Kim, Phthiraptera: Polyplacidae). Data were analyzed under an information-theoretic approach to identify factors predicting louse parasitism. Lice were most prevalent on plots with high densities of prairie dogs. At the scale of hosts, lice were most abundant on prairie dogs in poor body condition (with low mass:foot ratios) and prairie dogs harboring large numbers of fleas (Siphonaptera, mostly Oropsylla hirsuta (Baker, Siphonaptera: Ceratophyllidae) and Pulex simulans (Baker,Siphonaptera: Pulicidae)). Lice have been implicated as supplemental vectors of the primarily flea-borne bacterium Yersinia pestis (Yersin, Enterobacteriales: Yersiniaceae), a re-emerging pathogen that causes sylvatic plague in prairie dog populations. Coparasitism by lice and fleas, as found herein, might enhance plague transmission. L. cynomyis deserves attention in this context.

Organic carbon storage in floodplain soils of the U.S. prairies

AbstractTwo data sources, field‐collected samples and values in the NRCS SSURGO database, were used to estimate organic carbon concentration (%) and stock (Mg C/ha) in floodplain soils along rivers of the tallgrass and shortgrass prairie within the United States. Field sampling of 6 sites in the tallgrass prairie and 6 sites in the shortgrass prairie (total sample size of 370 vertical cores) indicates that percent organic carbon within a planar cross section through floodplain sediment at a site is spatially heterogeneous and does not decline systematically with depth, but statistical analyses indicate that soil organic carbon is randomly distributed. The median values of organic carbon concentration at both field‐sampled and sites remotely sampled based on soil maps in the tallgrass prairie are significantly higher than those of the shortgrass prairie. Median values of organic carbon stock are not significantly different between those obtained from forested sites for comparison and either shortgrass or tallgrass prairie sites but are significantly higher at tallgrass than at shortgrass prairie sites. These results are surprising because upland net primary productivity in prairies is lower than in forested sites. We infer that the historical abundance of floodplain wetlands in river corridors of the tallgrass prairie results in high organic carbon stocks in tallgrass prairie river corridors. This implies that management designed to enhance carbon sequestration should focus on floodplain soils, especially in the tallgrass prairie region, as well as on upland forests.

Surface coal mine permit application for successful reclamation, semi-arid shortgrass prairie (Wyoming, USA)

Approximately 40% of USA coal originates in an ecologically sensitive area of semi-arid shortgrass prairie in Wyoming. Before a surface coal mine can begin operation in the USA, it must secure a mining permit and comply with regulations and performance standards under the USA Surface Mining Control and Reclamation Act (SMCRA), other federal environmental acts, and state programs. The Wyoming Department of Environmental Quality (WDEQ)/Land Quality Division (LQD) administers Wyoming’s coal regulatory program. The permit application and bonding process for the largest surface coal mine permit in the USA, North Antelope Rochelle Mine (NARM) located in short grassland prairie in the northeast Wyoming, is discussed. The permit application process begins with the collection of baseline environmental data that characterizes premining conditions of the permit area. The permit application includes adjudication information, baseline information, mine and operation plans, and reclamation plans. Fulfillment of permit commitments and requirements of rules and regulations are inspected monthly by the LQD’s representative in the field. Before a mine permit is issued, the mine operator must submit a reclamation bond to secure the performance of reclamation obligations that is later revised annually. In Wyoming, four reclamation bond release phases indicate the completion of various stages of the reclamatikon process. NARM’s specific bond release verification cirteria, performance standards, and field verificatoins of bond release phases are discussed. The Bond Release Geodatabase (a GIS/GPS approach) was developed for this mine to monitor progress in meeting criteria and performance standards for incremental bond release. The Bond Release Geodatabase significantly reduces the time needed to track bond release progress, reach agreement between operator and regulator, and improve the state inspector’s ability to assess reclamation adequacy and progress.