Short Exposure Research Articles

Quantifying structural changes in organised biomineralized surfaces using synchrotron Polarisation-Induced Contrast X-ray Fluorescence.

The quantitative characterization of the structure of biomineral surfaces is needed for guiding regenerative strategies. Current techniques are compromised by a requirement for extensive sample preparation, limited length-scales, or the inability to repeatedly measure the same surface over time and monitor structural changes. We aim to address these deficiencies by developing Calcium (Ca) K-edge Polarisation Induced Contrast X-ray Fluorescence (PIC-XRF) to quantify hydroxyapatite (HAp) crystallite structural arrangements in high and low textured surfaces. Minimally prepared human dental enamel was used as an exemplar to quantify initial surface structures, and the disruption caused by short dietary acid exposures. By measuring surfaces at different rotational angles relative to a polarised focused (2×2 µm) monochromatic X-ray source (at either 4049.2 and 4051.1 eV) it was possible to discriminate the principal and secondary orientations of surface crystallites, along with their texture. It was also possible to quantify the organisation of crystallites in both low (enamel cross-sections) and highly textured (facial enamel) surfaces including the identification of crystallites aligned perpendicular to the surface-a challenge for other synchrotron techniques. Surface modifications following short term acid erosion (affecting <20 µm of the enamel surface depth) were detected as significant shifts in principal crystallite orientation (p < 0.001) and as a marked reduction in surface texture (p < 0.001). Findings suggest preferential dissolution of HAp based on crystallite angular orientation. We demonstrate that PIC-XRF is a powerful tool to quantify biomineral surfaces, with minimal sample preparation that enables monitoring of surface structural changes through repeated measurements. STATEMENT OF SIGNIFICANCE: This study introduces Calcium (Ca) K-edge Polarisation Induced Contrast X-ray Fluorescence (PIC-XRF) as a method for quantifying the structure of biomineral surfaces, addressing limitations in existing techniques that require extensive sample preparation and cannot repeatedly measure the same surface. By using minimally prepared dental enamel, PIC-XRF successfully discriminated between principal and secondary orientations of hydroxyapatite crystallites, including end-on crystallites-a challenge for other synchrotron methods. Additionally, PIC-XRF detected significant structural changes due to short-term acid erosion. This technique's potential to repeatedly and non-invasively analyze biomineral surfaces offers new opportunities for understanding surface dynamics and guiding regenerative treatments.

Impact of under-assisted ventilation on diaphragm function and structure in a porcine model.

Long-term controlled mechanical ventilation (CMV) in intensive care unit (ICU) induces ventilatory-induced-diaphragm-dysfunction (VIDD). The transition from CMV to assisted mechanical ventilation is a challenge that requires clinicians to balance over-assistance and under-assistance. While the effects of over-assistance on the diaphragm are well known, we aimed to assess the impact of under-assistance on diaphragm function and structure in piglet model with pre-existing VIDD (after long-term CMV) or without VIDD (short-term CMV). Twenty-two Large-White female piglets were anesthetized, ventilated, and separated into two groups: a VIDD group (n=10) with long-term 72-hour CMV, and a no-VIDD group (n=12) with short-term 2-hour CMV. After sedation reduction at the end of CMV period, each piglet was switched to under-assisted ventilation for 2 hours. Diaphragm function (supramaximal diaphragm pressure-generating capacity assessed by negative tracheal pressure after transvenous phrenic nerve stimulation) and diaphragm structure (mini-invasive in vivo biopsies) were assessed before and after under-assisted ventilation. In VIDD group, supramaximal diaphragm pressure-generating capacity decreased by 22% from 69.9±12.7 to 54.9±19.7 cmH2O (p=0.04) after 72 hours of CMV evidencing VIDD, then dropped by a further 29% from 54.9±19.7 to 38.9±15.5 cmH2O (p<0.01) after 2 hours of under-assisted ventilation. Diaphragm pressure-generating capacity remains stable from 55.3±22.7 to 58.2±24 cmH2O (p=0.24) in no-VIDD group. Diaphragm structure showed sarcomeric injuries increase from 13±10% to 24±19% (p<0.01) and lipid droplets decrease from 14±8% to 11±6% (p=0.03) of the total micrograph area after 2 hours of under-assisted ventilation in the VIDD group. Sarcomeric injuries and lipid droplets accounted respectively for 17±16% and 2±3% of the total micrograph area after under-assisted ventilation in the no-VIDD group. In this porcine model, a short two-hour exposure of under-assisted ventilation induces impairment of diaphragm function with damage to the diaphragm structure in ICU condition with pre-existing VIDD.

Effects of short and long-term exposure to benzene, toluene, ethylbenzene, and xylenes (BTEX) in indoor environment air on human health: A systematic review and meta-analysis

Today, because of the increasing level of people's need to improve well- being in social and individual life, air pollutants have been released that have Pollution harms both human health and the environment. This research examined Benzene, Toluene, Ethylbenzene, and Xylenes (BTEX) levels in indoor air across different global locations from 1963 to 2023. The investigation employed both; a systematic review and meta-analysis method. The health risks associated with long and short-terms exposure to benzene, toluene, ethylbenzene, and xylene were assessed. That average concentration benzene was 23.07 μg⁄m3, toluene was 131.60, ethylbenzene was 28.91, and xylene was 63.87. Also, the health risk assessment based on a 95% confidence level showed that the pollutants in question play a role in causing diseases such as lung neoplasm, stomach neoplasm, colon neoplasm, liver neoplasm, headache, dizziness, nausea, insomnia, etc. Consequently, it is crucial to implement stringent measures aimed at lowering the levels of these contaminants in indoor spaces.

Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis

PurposeImmune checkpoint blockades (ICBs) are promising, however they do not fit all types of tumor, such as those lack of tumor antigens. Induction of potent anti-tumor T cell immunity is critical for cancer therapy. In this study, we investigated the efficacy of immunotherapy via the immunogenic cell death (ICD) dying tumor cells in mouse models of lung metastasis and tumorigenesis.MethodsICD was induced by short exposure to lethal dose of chemotherapeutic drug doxorubicin (Dox), which initiated an irreversible ICD program in tumor cells. We immunized mice with ICD dying tumor cells in prevention, therapy in lung metastasis models, and Gprc5a-knockout (ko) model of lung tumorigenesis. T cells and macrophages isolated from lymph nodes or tumor tissues were analyzed by flow cytometry. Cytokines were analyzed by ELISA or Q-PCR analysis.ResultsImmunization with these live but ICD dying tumor cells induced potent tumor-specific anti-tumor T cell immunity, which not only protected host from challenge by these tumor cells in prevention and therapy in mouse model of lung metastasis, but also prevented tumors development in Gprc5a-ko mouse model of lung tumorigenesis. The lymphocytes from lymph nodes and tumor tissues exhibited greatly enhanced activities of Th1 cells and M1 macrophages.ConclusionImmunization with the ICD dying tumor cells evokes potent tumor-specific T cell immunity, which provides a novel approach for cancer immunotherapy.

Early developmental effects of propofol exposure in different stages of zebrafish embryos.

The mechanism of action of propofol, a common intravenous anaesthetic, in early life stages is not well understood with contradictory studies showing neurotoxic and neurogenic effects in the developing brain. Zebrafish early life stages have been established as an alternative model for animal experimentation with propofol toxicological effects reported following chronic exposure. Yet, the acute exposure to other anaesthetics has been shown to induce early life stage-dependent toxicological outcomes. Therefore, the present study aimed to evaluate the teratogenic effects of propofol at the 256-cell, 50 % epiboly and 1-4 somite stages following a 20 min exposure. Embryos were exposed after primarily assessment of propofol acute toxicity (24h-LC50=9.82 μg mL-1) and absorption at different developmental stages by chromatography. Embryos (2 hours post-fertilization, hpf) were treated with an anaesthetic and toxicological concentration of propofol (2.5 and 10 μg mL-1, respectively) for 20-min. Mortality and developmental toxicity were then evaluated until 144 hpf, when the behaviour and oxidative-stress-related biomarkers were assessed. Exposure at the 256-cell stage resulted in a concentration-dependent increased number of abnormalities in head, fins and tail and a decreased body length as well as in changes in ATPase activity for the lowest concentration. On the other hand, exposure at later stages resulted in a decreased survival while no significant malformations were detected. Yet, exposure during the 50 % epiboly stage resulted in the increase of ROS levels as well as glutathione (GST and GSSG) levels while exposure at 1-4 somite stage resulted in increased DNA damage and ATPase alterations. The behaviour of zebrafish was similar among treatments. Overall, these findings show highlight the stage-dependent teratogenic potential of short propofol exposures during zebrafish early development. The alterations observed may be linked to the activation of the zygotic transcription in embryos, requiring further studies to delve into the molecular changes underlying the observed effects.

Multi-generational exposure of Daphnia magna to pharmaceuticals: Effects on colonization, reproduction, and habitat selection behavior.

The presence of pharmaceuticals in the aquatic environment is increasing due to their growing use for human health. Although most studies are based on short exposures to these contaminants, the present study has emerged from the need to study pharmaceuticals in aquatic organisms over a long-term exposure to understand any multi-generational chronic effects and alterations regarding habitat selection. Therefore, this study shows: (1) the ability of Daphnia magna to colonize environments contaminated with caffeine, ibuprofen and fluoxetine, and (2) the effect of these pharmaceuticals on reproduction and habitat selection (under two scenarios: with and without food) after a long-term exposure period of three generations. It was observed that caffeine shortened the time between generations and caused an increase in the number of neonates per female. The opposite was observed with ibuprofen: the time to reach the third F3 generation was double when compared to those exposed to caffeine. Fluoxetine did not alter the reproduction, nor was repellent/attractive for daphnids. In the habitat selection tests, organisms cultivated in clean water preferred the compartment with caffeine, highlighting its attractive effect. Caffeine was also attractive for daphnids in the colonization test. Apart from this, no chemical showed any attractive or repulsive effect in the absence of food during the habitat selection tests. Our findings show that the presence of some pharmaceuticals could cause alterations in distribution and habitat selection patterns, and a significant effect on the reproduction of this species. underlining the importance of studying the effects of contamination by long-term exposure.

Analysing the interplay of regional economic structures and COVID-19's impact on Irish regions

ABSTRACT This paper analyses the regional economic impacts of COVID-19 in Ireland. The results show regional labour market impacts were related to pre-existing economic structures rather than infection rates. Drivers of the employment shock were found to be regional specialisms in tourism focused sectors. Cushions were provided by regional specialisms in agriculture, healthcare and the knowledge intensive service sectors. For more rural regions that appeared more resilient to the labour market shock, cushions were likely provided by employment in slower growth potential sectors. During the recovery phase, these cushions can become anchors contributing to regional divergence. Consequently, some regions less severely impacted by the pandemic shock may, in fact, face more difficult structural challenges in the longer term. The pandemic has thus exacerbated pre-existing structural issues that are likely related to the observed regional economic divergence and findings of regional development traps since the recovery from the previous recessionary period of the 2010s. The broad implication is that that the regional recovery from the pandemic will not be based purely on short term COVID-19 exposure. In general terms, regional policy should look beyond the initial pandemic shocks to the key underlying development issues. A central point here is that there are no quick transformational fixes rather long-term structural imbalances need to be met. The key policy challenge, in the Irish case, is to avoid the trend from the previous economic crisis of regional divergence during the recovery phase.

Do Endangered Glacial Relicts Have a Chance for Effective Conservation in the Age of Global Warming? A Case Study: Salix lapponum in Eastern Poland.

The abiotic stresses to which plants are exposed, especially in times of climate change, can result in the disruption of natural plant physiological processes. Sudden atmospheric phenomena may increase the risk of failure in protecting rare and extinction-threatened plant species by translocation. This study aimed to determine the effect of extreme ambient temperatures on the condition and physiological response of Salix lapponum plantlets used for their reintroduction into the natural habitat. Salix lapponum plants obtained by micropropagation methods at different stages of growth under laboratory conditions were subjected to a biological experiment. Plants were exposed for 12 h to temperature extremes (0 °C and 30 °C), after which the values of selected markers of the biochemical response were determined, such as photosynthetic pigments and anthocyanin content, guaiacol peroxidase and catalase activity, the presence of ROS and the RWC value. The study showed that plants at early growth stages were sensitive to low-temperature stress. In contrast, older ones showed a stronger response to high temperature, marked by an increased anthocyanin content and guaiacol peroxidase activity. It was also found that a short exposure to temperature extremes did not change the photosynthetic pigment content or catalase activity. The results of the study may be an important indication for the optimization of plant acclimatization methods in the process of their active protection by species translocation.

Exogenous acetate attenuates inflammatory responses through HIF-1α-dependent glycolysis regulation in macrophage

Cytokine storm is a hallmark for acute systemic inflammatory disease like sepsis. Intrinsic microbiome-derived short-chain fatty acid (SCFAs) like acetate modulates immune cell function and metabolism has been well studied. However, it remains poorly investigated about the effects and the underlying mechanism of exogenous acetate in acute inflammation like sepsis. Here, we observed that serum acetate accumulates in patients undergoing abdominal gastrointestinal surgery and in septic mice. Short exposure to high-dose exogenous acetate protects mice from sepsis by inhibiting glycolysis in macrophages, both in vivo and in vitro. Hypoxia-inducible factor 1 subunit alpha (HIF-1α) stabilization or overexpression reverses the decreased glycolysis and pro-inflammatory cytokine production in macrophages and abrogates acetate’s protective effect in septic mice. Meanwhile, we also found acetyl-CoA synthetase-2, but not GPR41 or GPR43, plays a key role in acetate’s immunosuppressive effect. Acetate transiently increases acetyl-coenzyme A production, promoting histone acetylation and decreasing acetyl-transfer to NF-κB p65. These findings suggest that short exposure to mM-level acetate inhibits macrophage immune response linked to HIF-1α-dependent glycolysis. Taken together, we demonstrate short-term exposure of exogenous acetate could regulate inflammatory responses through attenuating HIF-1α-dependent glycolysis.

Assessment of total mercury content in fish muscle tissue from the middle basin of the Pastaza River, Ecuador.

One of the most toxic metals is mercury, which exhibits high toxicity during short exposure periods. This study aimed to determine the concentration of total mercury (THg) in fish muscle tissue from various species captured from two locations in the middle basin of the Pastaza River in Ecuador, which the locals use in their weekly diet. The individuals captured belong to the following families: Loricariidae, Cetopsidae, Heptapteridae, Cichlidae, Parodontidae, Characidae, Prochilodontidae, Curimatida, Siluriformes, Cichliformes, Characiformes, Erythrinidae and Curimatidae. Carnivorous species Charax sp. and Cetopsis plumbea, had THg concentrations were 0.241± 0.018 and 0.116 ± 0.045 mg kg-1, respectively. Herbivorous species Hypostomus sp. had a lower of THg. Carnivorous species Hoplias malabaricus exhibited THg concentration of 0.160±0.033 and 0.020±0.007 mg kg-1 for the omnivorous species Creagrutus sp. Carnivorous species accumulated higher levels of Hg compared to non-carnivorous species. Concentration of total mercury in the collected fish did not exceed the maximum permissible limits set by legislative standards (Ecuadorian Institute of Standardization (INEN as per the acronym in Spanish), FAO/WHO and European Commission); and the objective hazard quotient was less than 1, indicating that the consumption of this fish may pose no risk to human health. Considering the mean concentrations of methylmercury, for all the fish species analysed, the results for daily consumption rate were between 6-199 g and 29-928 g for children and adults, respectively.

The Motility of Mouse Spermatozoa Changes Differentially After 30-Minute Exposure Under Simulating Weightlessness and Hypergravity.

Research into the mechanisms by which gravity influences spermatozoa has implications for maintaining the species in deep space exploration and may provide new approaches to reproductive technologies on Earth. Changes in the speed of mouse spermatozoa after 30 min exposure to simulated weightlessness (by 3D-clinostat) and 2 g hypergravity (by centrifugation) were studied using inhibitory analysis. Simulated microgravity after 30 min led to an increase in the speed of spermatozoa and against the background of an increase in the relative calcium content in the cytoplasm. This effect was prevented by the introduction of 6-(dimethylamino) purine, wortmannin, and calyculin A. Hypergravity led to a decrease in the speed of spermatozoa movement, which was prevented by sodium orthovanadate and calyculin A. At the same time, under microgravity conditions, there was a redistribution of proteins forming microfilament bundles between the membrane and cytoplasmic compartments and under hypergravity conditions-proteins forming networks. The obtained results indicate that even a short exposure of spermatozoa to altered gravity leads to the launch of mechanotransduction pathways in them and a change in motility.

Short Exposure to Full Moonlight Has a Long-Term Impact on Brassica juncea Cell Activity and Growth.

Lunar farming, often regarded as a myth, is regularly practiced in many places around the world (e.g., India) where framers organized their agricultural activities according to moon phases. Early and recent work showed that exposure to moonlight affects the life cycle of plants, from seed germination and vegetative growth to fruit maturation and dispersal. Here we addressed the long-term effect of short exposure to full moonlight (FML) on cellular activities in Brassica juncea by analyzing protein and metabolite profiles immediately after 3-night-exposure (3NE) or 7 and 15 days after exposure (DAE) to FML. This study shows an increase in nuclear size following 3NE to FML, which was accompanied by changes in protein and metabolite profiles. We identified significant alterations in protein and metabolite profiles between FML and dark-treated plants in conjunction with developmental stages, which persisted long after exposure to FML. Most notable are the changes in composition of metabolite interconversion enzymes (MIEs) at various developmental stages which were intensified in FML-treated plants. Changes in MIEs were accompanied by significant alterations in metabolite composition and level, particularly at 15DAE, including branched-chain amino acids (e.g., valine, leucine), multiple sugars (raffinose, glucose, sucrose) as well as the tricarboxylic acid (TCA) cycle intermediates malic acid and citric acid. Thus, our results show that short-term exposure to FML triggers a developmental switch resulting in a long-term impact on plant performance that brings about an increase in cell activities and consequently enhanced growth. Our results call for meticulous research on this lunar phenomenon and its potential to enhance crop plant growth and development.

Changes in Plasma Levels of Prestin and Otolin-1 in Dental Students.

Dentists and dental professionals report a high prevalence of noise-induced hearing loss (NIHL) and related symptoms. Chronic exposure to high-frequency dental instrument sounds, which can damage the outer hair cells (OHCs) of the cochlea, is strongly linked to their NIHL. Similarly, dental students in teaching clinics often report symptoms associated with NIHL. In this study, we measured plasma levels of prestin and otolin-1, two biomarkers associated with inner ear health, in first-year (D1) and third-year (D3) dental students. D1 students were selected for their relatively short exposure to dental clinics, while D3 students represented a group with substantial cumulative exposure. First-year (M1) medical students, who have no exposure to dental instruments, served as the negative controls, while dental faculty, many of whom are diagnosed with NIHL or self-report hearing problems, served as the positive controls. Thirty-one students (D1=11, D3=8, M1=12) and 10 dental faculty volunteered for the study. Participating students completed an online survey about their hearing health, exposure to excessive sounds within and outside school, and use of hearing protection. Sound levels of medical Osteopathic Manipulative Medicine (OMM) and dental Simulation (SIM) labs while in session were recorded with a calibrated sound meter. Plasma levels of prestin and otolin-1 were quantitated using commercial ELISA kits. The sound level of in-session OMM lab was significantly higher (72.64 ± 1.69 dBA) than SIM lab (65.37 ± 3.97 dBA). Both medical and dental students experienced similar exposure to outside school noises; however, dental students had significantly longer exposure to high-frequency dental instrument sounds in the SIM lab (12.3 hours/week; 74.93-80.51 dBA). Plasma prestin levels were lowest in D3 students (559.88 ± 24.91 ng/ml), followed by D1 students (576.27 ± 71.44 ng/ml). Negative control M1 students had higher levels (675.73 ± 90.23 ng/ml), while the positive control dental faculty group exhibited the highest prestin levels (727.71 ± 128.65 ng/ml). Prestin levels in D3 students were significantly lower than those in M1 students, and the dental faculty group had significantly higher prestin levels than both D1 and D3 students. No differences in otolin-1 levels were observed among the groups. Our finding that D3 students, with greater experience using dental instruments in the SIM lab, exhibited the lowest plasma prestin levels may indicate a protective mechanism by the OHCs, downregulating its expression to reduce the risk of NIHL. This is in line with the findings in the dental faculty, who self-reported NIHL or hearing problems and exhibited the highest prestin levels. The lack of changes in plasma otolin-1 levels across groups, combined with students self-reporting excellent hearing health, may offer alternate view of subclinical inner ear damage among dental students. Future studies incorporating audiometry and otoacoustic emission tests along with the inner ear biomarkers may provide better understanding of NIHL in dental students.

Molecular and Functional Characterization of the Peritoneal Mesothelium, a Barrier for Solute Transport.

Peritoneal dialysis (PD) is an increasingly needed, life-maintaining kidney replacement therapy; efficient solute transport is critical for patient outcome. While the role of peritoneal perfusion on solute transport in PD has been described, the role of cellular barriers is uncertain, the mesothelium has been considered irrelevant. We calculated peritoneal blood microvascular endothelial (BESA) to mesothelial surface area (MSA) ratio in human peritonea in health, chronic kidney disease, and on PD, and performed molecular transport related gene profiling and single molecule localization microscopy in two mesothelial (MC) and two endothelial cell lines (EC). Molecular-weight dependent transport was studied in-vitro, ex-vivo and in mice. Peritoneal BESA is 1-3-fold higher than MSA across age groups, and increases with PD, while the mesothelium is preserved during the first two years of PD. Tight junction, transmembrane and transcytotic transporter expression are cell-type specifically expressed. At nanoscale, tight junction anchoring protein Zonula occludens-1 is more abundant and more continuously expressed along the MC than the EC. Ionic conductance is 3-fold lower across the MC than human microvascular EC, as is the permeability for creatinine, 4- and 10-kDa, but not for 70-kDa dextran. MC removal from sheep peritoneum abolishes ionic barrier function. Short term intraperitoneal LPS exposure in mice selectively affects peritoneal mesothelial integrity and increases transperitoneal solute transport. We provide molecular correlates and consistent functional evidence for the mesothelium as a barrier for peritoneal solute transport, i.e., essential information on peritoneal transport modelling, and for interventions to improve PD efficiency and biocompatibility, and beyond.

Neuroeffects of Microwave EMF Range of Non-Thermal Intensity and Short Exposure

The article presents a generalization and analysis of the results of many years of research by the author of the neuroeffects of weak electromagnetic effects in order to answer the questions: do they exist, what are their phenomenology, neurophysiological mechanism, ways of modification? Using electrophysiological and behavioral research methods, the line of effects of weak EMF from the neuron to the brain, the central nervous system (as a system) and the body is shown. In animal experiments and studies with the participation of volunteer testers, their phenomenology, dependence on parameters, modulation mode, and EMF organization are statistically substantiated. As a result, it is concluded that this is a common stimulus of the central nervous system, which in its influence on the body obeys the laws of physiology about the biological significance of the response, ways of its modification and adaptation mechanisms, which is advisable to take into account.

Researches on projectile muzzle velocity measurement system based on high-speed camera binocular intersection measurement and laser illumination

Abstract In order to reduce the impact of muzzle flame and smoke blurring on the flight trajectory imaging quality of projectile when measuring muzzle velocity in current weapon testing systems, a projectile velocity measurement system based on sequential pulsed laser illumination was studied. The wavelength of the pulsed laser is 532nm and the narrow-band filters with the same center wavelength were put in front of the camera lens which was used to suppress the influence of the flame. The pulsed laser and the filter helped the system to capture the images of the muzzle projectile wrapped in flames. The spatial movement distance of the projectile was calculated based on the principle of binocular intersection. The flying time of the projectile was determined by the high-speed camera shooting rate and the image frames intervals. The system was used to test the muzzle velocity of the projectile fired from a 12.7mm ballistic gun. The experiment results show that laser illumination effectively suppressed the influence of muzzle flames on projectile imaging. According to the calibration and binocular vision imaging algorithms, the muzzle velocity of the projectile was calculated with an accuracy of better than 0.3%. The method can measure the initial velocity of the projectile with the help of sequential pulsed laser illumination and short exposure time (as short as 1.1μs), which has great application value for range testing.

Angiotensin II Exposure In Vitro Reduces High Salt-Induced Reactive Oxygen Species Production and Modulates Cell Adhesion Molecules' Expression in Human Aortic Endothelial Cell Line.

Background/Objectives: Increased sodium chloride (NaCl) intake led to leukocyte activation and impaired vasodilatation via increased oxidative stress in human/animal models. Interestingly, subpressor doses of angiotensin II (AngII) restored endothelium-dependent vascular reactivity, which was impaired in a high-salt (HS) diet in animal models. Therefore, the present study aimed to assess the effects of AngII exposure following high salt (HS) loading on endothelial cells' (ECs') viability, activation, and reactive oxygen species (ROS) production. Methods: The fifth passage of human aortic endothelial cells (HAECs) was cultured for 24, 48, and 72 h with NaCl, namely, the control (270 mOsmol/kg), HS320 (320 mOsmol/kg), and HS350 (350 mOsmol/kg). AngII was administered at the half-time of the NaCl incubation (10-4-10-7 mol/L). Results: The cell viability was significantly reduced after 24 h in the HS350 group and in all groups after longer incubation. AngII partly preserved the viability in the HAECs with shorter exposure and lower concentrations of NaCl. Intracellular hydrogen peroxide (H2O2) and peroxynitrite (ONOO-) significantly increased in the HS320 group following AngII exposure compared to the control, while it decreased in the HS350 group compared to the HS control. A significant decrease in superoxide anion (O2.-) formation was observed following AngII exposure at 10-5, 10-6, and 10-7 mol/L for both HS groups. There was a significant decrease in intracellular adhesion molecule 1 (ICAM-1) and endoglin expression in both groups following treatment with 10-4 and 10-5 mol/L of AngII. Conclusions: The results demonstrated that AngII significantly reduced ROS production at HS350 concentrations and modulated the viability, proliferation, and activation states in ECs.

Rapid changes in stress-related gene expression after short exposure of Arabidopsis leaves to cold plasma

Cold Atmospheric Plasma (CAP) technology has emerged as a promising tool in various biological applications due to its ability to generate a composite signal comprising reactive oxygen and nitrogen species, ultraviolet radiation, and electromagnetic fields, all while maintaining a stable temperature. Although CAP treatments have demonstrated significant effects on seed germination and plant growth, the direct molecular responses of plants to CAP exposure remain poorly understood. In this study, young Arabidopsis thaliana leaves were exposed to a brief 5- or 30-s localized CAP treatment, resulting in rapid and localized tissue damage without causing lethal effects on the entire plant. Molecular analyses conducted on the entire plant rosette revealed a notable increase in hydrogen peroxide levels, along with the upregulation of stress-related genes, akin to a wound response. Of particular interest, the activation of RelA/SpoT Homolog (RSH) genes encoding proteins that regulate the synthesis of the stress marker (p)ppGpp, also known as alarmone, and playing a major role in the energic regulation of photosynthesis, occurred shortly after CAP exposure. The expression of RSH genes was up-regulated after 5s CAP exposure, while the wound stress marker ZAT12 remained unaffected, highlighting a specific signalling pathway to activate RSH genes. This finding suggests the potential involvement of the alarmone signalling pathway in the plant's response to CAP exposure, thereby opening avenues for further exploration of metabolic pathways and signalling cascades induced by CAP treatment.

Changes in Wood Plastic Composite Properties After Natural Weathering and Potential Microplastic Formation

Wood plastic composites (WPCs) have recently gained attention as alternatives to traditional wood materials for outdoor use, thanks to their enhanced moisture resistance and durability, which extends their service life. Discolouration as well as surface erosion has been observed during weathering for both WPCs with untreated and heat-treated wood. However, aspects such as changes in surface hydrophobicity, chemistry, and erosion in terms of microplastic formation have received less attention; this research aimed to evaluate these factors during natural weathering. Four types of WPC samples, consisting of 50% wood particles (untreated and heat-treated) and 50% polypropylene, were naturally weathered in Latvia for two years. The samples measured 240 mm × 240 mm × 5 mm. Results showed rapid colour changes, microcracks, and exposed wood particles, suggesting microplastic formation. ATR-FTIR analysis showed increased absorption at 1715 cm⁻¹ (carbonyl groups) and at 3410 cm−1 and 3460 cm−1, typical of wood, indicating chemical changes on the surface. These changes influenced surface hydrophobicity, roughness, and water penetration. In a relatively short exposure time, WPCs without proper additives undergo significant changes in their aesthetic and physical properties, leading to surface erosion and potential microplastic formation. This could challenge the perception of WPCs as environmentally friendly materials.

The Posterior Dominant Rhythm Remains Within Normal Limits in the Microgravity Environment.

Electroencephalogram (EEG) biomarkers with adequate sensitivity and specificity to reflect the brain's health status can become indispensable for health monitoring during prolonged missions in space. The objective of our study was to assess whether the basic features of the posterior dominant rhythm (PDR) change under microgravity conditions compared to earth-based scalp EEG recordings. Three crew members during the 16-day AXIOM-1 mission to the International Space Station (ISS), underwent scalp EEG recordings before, during, and after the mission by means of a dry-electrode self-donning headgear designed to support long-term EEG recordings in space. Resting-state recordings were performed with eyes open and closed during relaxed wakefulness. The electrodes representative of EEG activity in each occipital lobe were used, and consecutive PDR oscillations were identified during periods of eye closure. In turn, cursor-based markers were placed at the negative peak of each sinusoidal wave of the PDR. Waveform averaging and time-frequency analysis were performed for all PDR samples for the respective pre-mission, mission, and post-mission EEGs. No significant differences were found in the mean frequency of the PDR in any of the crew subjects between their EEG on the ISS and their pre- or post-mission EEG on ground level. The PDR oscillations varied over a ±1Hz standard deviation range. Similarly, no significant differences were found in PDR's power spectral density. Our study shows that the spectral features of the PDR remain within normal limits in a short exposure to the microgravity environment, with its frequency manifesting within an acceptable ±1 Hz variation from the pre-mission mean. Further investigations for EEG features and markers reflecting the human brain neurophysiology during space missions are required.