Short Electric Field Pulses Research Articles

One- and two-photon excitation dynamics using semiclassical electron force field model.

We have extended the semiclassical-based electron force-field simulation by introducing field-electron interaction to enable us to describe linear and nonlinear electronic excitation dynamics of a condensed matter system with low computational cost. To verify the simulation method, as a first step, numerical examples of interaction dynamics of simple systems (H atom, SiH4 molecule, and Si crystalline solid) with applied short electric field pulse as well as the obtained absorbed energies by the one- and two-photon excitations have been reported along with comparison with quantum dynamics calculations as reference.

Cardiomyocytes Permeabilization and Electrotransfection by Unipolar and Bipolar Asymmetric Electric Field Pulses.

Short electric field pulses represent a novel potential approach for achieving uniform electroporation within tissue containing elongated cells oriented in various directions, such as electroporation-based cardiac ablation procedures. In this study, we investigated how electroporation with nanosecond pulses with respect to different pulse shapes (unipolar, bipolar, and asymmetric) influences cardiomyocyte permeabilization and gene transfer. For this purpose, rat cardiomyocytes (H9c2) were used. The efficacy of the pulsed electric field protocols was assessed by flow cytometry and electrogene transfer by fluorescent and holotomographic microscopy. The response of the cells was assessed by the metabolic activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide[MTT] assay), F-actin distribution in cells by confocal microscopy, and muscle atrophy F-box (MAFbx) marker. We show nano- and microsecond pulse protocols, which are not cytotoxic for cardiac muscle cells and can be efficiently used for gene electrotransfection. Asymmetric nanosecond pulsed electric fields were similarly efficient in plasmid delivery as microsecond and millisecond protocols. However, the millisecond protocol induced a higher MAFbx expression in H9c2 cells.

Electroporation Pulse Generator for Biomedical Applications With Improved Output Voltage Ripple and Reduced Bus Capacitor

Electroporation is a promising cancer treatment based on applying short electric field pulses. In this context, highly versatile pulse generators are required to generate high-voltage low-ripple pulses in the µs range. In the past, several multi-level converters have been proposed to achieve this aim. Achieving constant output voltage and, consequently, electric field is essential to obtain predictable treatments and minimize the risks of tumor relapse. However, significant bus capacitance is required to achieve the required performance, leading to bulky equipment with serious safety implications due to the stored energy. In this letter, a high-performance pulse generator for electroporation is proposed that takes advantage of a multi-level structure with linear voltage regulation to achieve the desired performance with reduced bus capacitance. The proposed converter has been designed and tested experimentally during both laboratory and operation room treatments.

Impact ionization and multiple photon absorption in the two-dimensional photoexcited Hubbard model

We study the non-equilibrium response of a 4x3 Hubbard model at U=8 under the influence of a short electric field pulse, with the main focus on multiple photon excitations and on the change of double occupancy after the pulse. The behavior mainly depends on the driving frequency of the electric field. The largest change of double occupancy occurs during the pulse. For frequencies below the Mott gap, we observe multiphoton excitations at large field intensities. For frequencies beyond the gap energy, there is a region where Auger recombination reduces the double occupancy after the pulse. Impact ionization (Multi Exciton Generation), namely a growing double occupancy after the pulse, occurs for frequencies larger than twice the Mott gap. From the Loschmidt amplitude we compute the eigenstate spectrum of the quantum state after the pulse, observing multiple distinct photon excitation peaks, in line with expectations from a quasiparticle picture. We introduce a technique with which we analyze the time evolution of double occupancy in each peak individually. The long-term behavior of the double occupancy almost only depends on the absorbed energy, and we explore the connection of this property to the Eigenstate Thermalization Hypothesis.

Transport and Spectroscopy in Conjugated Molecules: Two Properties and a Single Rationale.

In the context of electron dynamics simulations, when the charge density of a molecule is subject to a perturbation in the form of a short electric field pulse, density fluctuations develop in time. In the absence of dissipation, these oscillations continue indefinitely, reflecting the resonances of the electronic system; as a matter of fact, from the Fourier transform of the time dependent dipole arising from them, the absorption spectrum of the molecule can be calculated. Since these oscillations are the result of the electrons moving through the molecular structrure, it seems plausible that they carry information on the transport properties of the system. This is the idea explored in the present article for the case of conjugated polymers. Specifically, we depart from a nonequilibrium state with the charge concentrated on the ends of the molecule, and estimate the currents flowing back and forth during the evolution of electron dynamics simulations. These show that the charge oscillates between the sides of the polymer with the predominance of a frequency that is coincident with one of the main bands in the absorption spectrum, which can be ascribed to a charge transfer transition. Thus, from the charge transfer band frequency appearing in the absorption spectrum, the molecular conductance of a conjugated molecule can be calculated. Also interestingly, we find that, while a perturbation excites all resonances of an electronic system, the form in which this perturbation is applied can be manipulated to determine the relative manifestation of the response. The electric field pulse excites all resonances according to the transition dipole moment and is then appropriate to produce the absorption spectrum. A charge separated initial state, however, specifically stimulates the charge transfer mode and is then suitable to calculate transport properties. This allows us to propose a simple approach to obtain molecular conductances and tunneling decay constants in agreement with results from much more demanding electronic structure techniques.

Impedance spectroscopy study of the retinal pigment epithelium: Application to the monitoring of blue light exposure effect on A2E-loaded in-vitro cell cultures

In age-related macular degeneration, the retinal pigment epithelium can be damaged by light acting on photosensitizers like N-retinylidene-N-retinylethanolamine (A2E). In this paper, the underlying cellular mechanism of lesion at the cell layer scale is analyzed by impedance spectroscopy. Retinal pigment epithelium (RPE) cells are cultured on top of custom-made electrodes capable of taking impedance measurements, with the help of a custom-made electronic setup but without the use of any chemical markers. An incubator is used to house the cells growing on the electrodes. An electrical model circuit is presented and linked to the constituents of the cell layer in which various electrical elements have been defined including a constant phase element (CPE) associated to the interface between the cell layer and the electrolyte. Their values are extracted from the fitted model of the measured impedance spectra. In this paper, we first investigate which parameters of the model can be analyzed independently. In that way, the parameter's evolution is examined with respect to two different targeted changes of the epithelium: 1. degradation of tight junctions between cells by extracellular calcium sequestration with Ethylenediaminetetraacetic acid (EDTA); 2. application of high amplitude short length electric field pulses. Based on the results obtained showing a clear relation between the model and the physiological state of the cell layer, the same procedure is applied to blue light exposure experiment. When A2E-loaded cells are exposed to blue light, the model parameters indicate, as expected, a clear degradation of the cell layer opposed to a relative stability of the not loaded ones.

Ultrafast polarization switching of (BaSr)TiO3 thin film by a single-period terahertz pulse in a vicinity of phase transition

We report here an experimental and theoretical study on ultrafast switching of dielectric polarization in (Ba0.8Sr0.2)TiO3 thin films by a strong electric field of a nearly single-cycle THz pulse at different temperatures. Optical second harmonic generation (SHG) is used as a probe of the polarization in the terahertz pump-optical probe experiment. We describe an approach to interpretation of SHG response from the ferroelectric polydomain film excited by a short electric field pulse. We show that dielectric polarization induced by the THz field decreases while approaching the Curie temperature. Simulations with the help of the Duffing equation reproduce the experimental observations.

Nanopore occlusion: A biophysical mechanism for bipolar cancellation in cell membranes

Extraordinarily large but short electric field pulses are reported by many experiments to cause bipolar cancellation (BPC). This unusual cell response occurs if a first pulse is followed by a second pulse with opposite polarity. Possibly universal, BPC presently lacks a mechanistic explanation. Multiple versions of the “standard model” of cell electroporation (EP) fail to account for BPC. Here we show, for the first time, how an extension of the standard model can account for a key experimental observation that essentially defines BPC: the amount of a tracer that enters a cell, and how tracer influx can be decreased by the second part of a bipolar pulse. The extended model can also account for the recovery of BPC wherein the extent of BPC is diminished if the spacing between the first and second pulses is increased. Our approach is reverse engineering, meaning that we identify and introduce an additional biophysical mechanism that allows pore transport to change. We hypothesize that occluding molecules from outside the membrane enter or relocate within a pore. Significantly, the additional mechanism is fundamental and general, involving a combination of partitioning and hindrance. Molecules near the membrane can enter pores to block transport of tracer molecules while still passing small ions (charge number ±1) that govern electrical behavior. Our extension of the standard model accounts for key BPC behavior.

Observation of a strong-coupling effect on electron-ion collisions in ultracold plasmas.

Ultracold plasmas (UCPs) provide a well-controlled system for studying multiple aspects in plasma physics that include collisions and strong-coupling effects. By applying a short electric field pulse to an UCP, a plasma electron center-of-mass oscillation can be initiated. For accessible parameter ranges, the damping rate of this oscillation is determined by the electron-ion collision rate. We performed measurements of the oscillation damping rate with such parameters and compared the measured rates to both a molecular dynamics (MD) simulation that includes strong-coupling effects and a Monte Carlo binary collision simulation designed to predict the damping rate including only weak-coupling considerations. We found agreement between the experimentally measured damping rate and the MD result. This agreement did require including the influence of a previously unreported UCP heating mechanism whereby the presence of a dc electric field during ionization increased the electron temperature, but estimations and simulations indicate that such a heating mechanism should be present for our parameters. The measured damping rate at our coldest electron temperature conditions was much faster than the weak-coupling prediction obtained from the Monte Carlo binary collision simulation, which indicates the presence of a significant strong-coupling influence. The density averaged electron strong-coupling parameter Γ measured at our coldest electron temperature conditions was 0.35(8).

Damping of electron center-of-mass oscillation in ultracold plasmas

Applying a short electric field pulse to an ultracold plasma induces an electron plasma oscillation. This manifests itself as an oscillation of the electron center of mass around the ion center of mass in the ultracold plasma. In general, the oscillation can damp due to either collisionless or collisional mechanisms, or a combination of the both. To investigate the nature of oscillation damping in ultracold plasmas, we developed a molecular dynamics model of the ultracold plasma electrons. Through this model, we found that depending on the neutrality of the ultracold plasma and the size of an applied DC electric field, there are some parameter ranges where the damping is primarily collisional and some primarily collisionless. We conducted experiments to compare the measured damping rate with theory predictions and found them to be in good agreement. Extension of our measurements to different parameter ranges should enable studies for strong-coupling influence on electron-ion collision rates.

The electron transport within bulk wurtzite zinc oxide in response to strong applied electric field pulses

ABSTRACTStrong short electric field pulses are used to generate broadband terahertz radiation. Understanding the transport properties under such conditions is very important for the understanding of numerous terahertz photonic and electronic devices. In this paper, we report on transport simulations of the electrons within bulk wurtzite zinc oxide for pulsed high electric fields, with pulse durations of up to 400 fs. We focus on how key electron transport characteristics, namely the drift velocity and the corresponding average energy, vary with time since the onset of the pulse. For sufficiently high-field strength selections, we find that both of these parameters exhibit peaks. In addition, an electron drift velocity undershoot is observed following this peak. A contrast with the case of gallium nitride is considered; undershoot is not observed for the case of this material. Reasons for these differences in behavior are suggested.

A microfluidic device with removable packaging for the real time visualisation of intracellular effects of nanosecond electrical pulses on adherent cells

The biological mechanisms induced by the application of nanosecond pulsed electric fields (nsPEFs: high electrical field amplitude during very short duration) on cells remain partly misunderstood. In this context, there is an increasing need for tools that allow the delivering of such pulses with the possibility to monitor their effects in real-time. Thanks to miniaturization and technology capabilities, microtechnologies offer great potential to address this issue. We report here the design and fabrication of a microfluidic device optimized for the delivery of ultra short (10 ns) and intense (up to 280 kV cm(-1)) electrical pulses on adherent cells, and the real time monitoring of their intracellular effects. Ultra short electric field pulses (nsPEFs or nanopulses) affect both the cell membrane and the intracellular organelles of the cells. In particular, intracellular release of calcium from the endoplasmic reticulum was detected in real time using the device, after exposure of adherent cells to these nsPEFs. The high intensity and spatial homogeneity of the electric field could be achieved in the device thanks to the miniaturization and the use of thick (25 μm) electroplated electrodes, disposed on a quartz substrate whose transparency allowed real time monitoring of the nsPEFs effects. The proposed biochip is compatible with cell culture glass slides that can be placed on the chip after separate culture of several days prior to exposure. This device allows the easy exposure of almost any kind of attached cells and the monitoring in real time while exposed to nsPEFs, opening large possibilities for potential use of the developed biochips.

Generation of electrostatic solitary waves in the plasma sheet boundary layer

[1] Generation of electrostatic solitary waves (ESWs) in the plasma sheet boundary layer in terms of electron-acoustic solitons and double layers is proposed. The plasma sheet boundary layer is treated as a multicomponent magnetized plasma consisting of background electrons, counter-streaming electron beams and ions. The model is based on the multifluid equations and the Poisson equation, and uses the Sagdeev pseudo-potential technique. For the plasma parameters at the time of broadband electrostatic noise in the plasma sheet boundary layer observed by Cluster on 22 September 2004, the model predicts solitons/double layer with electric field ∼(0.01–30) mV/m with time durations ∼(0.1–4.5) ms. Such short electric field pulses, when Fourier transformed to the frequency domain, can appear as broadband electrostatic noise in the frequency range of ∼220 Hz to 10 kHz. It is proposed that the model can be a good candidate for explaining the generation of broadband electrostatic noise in the plasma sheet boundary layer.

Creating and Manipulating Vortices in Atomic Wave Functions with Short Electric Field Pulses

We demonstrate the creation of vortices in the electronic probability density of an atom subject to short electric field pulses, how these vortices evolve and can be manipulated by varying the applied pulses, and that they persist to macroscopic distances in the spectrum of ejected electrons. This opens the possibility to use practical femtosecond or shorter laser pulses to create and manipulate these vortex quasiparticles at the atomic scale and observe them in the laboratory. Within a hydrodynamic interpretation we also show, since the Schrödinger equation is a particular instance of the Navier-Stokes equations, that for compressible fluids vortices can appear spontaneously and with a certain time delay, which is not expected to occur from the conventional point of view, illustrating applicability of the present study to vortex formation more broadly.

Electrical modeling of the influence of medium conductivity on electroporation

Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. Experimental data show that the amount of permeabilization depends on the conductivity of the extracellular medium. If medium conductivity decreases then it is necessary to deliver a pulse of larger field amplitude in order to achieve the same effect. Models that do not take into account the permeabilization effect on the membrane conductivity cannot reproduce qualitatively the experimental observations. Here we employ an exponential function for describing the strong dependence of membrane conductivity on transmembrane potential. Combining that model with numerical methods we demonstrate that the dependence on medium conductivity can be explained as being the result of increased membrane conductance due to electroporation. As experimentally observed, extracellular conductivities of about 1 and 0.1 S m(-1) yield very similar results, however, for lower conductivities (<0.01 S m(-1)) the model predicts that significantly higher field magnitudes will be required to achieve the same amount of permeabilization.

Granulomatous reaction after an aesthetic electroporation procedure

Auteur(s) : Clara De Simone, Teresa Sisto, Selenia Vallone, Angelo Carbone, Maurizio Rotoli, Pierluigi Amerio Department of Dermatology, Catholic University of the Sacred Heart, Largo Gemelli 8, 00168 Rome Italy Electroporation produces transient hydrophilic pores across cell membranes through application of short electric field pulses. The pores allow the passage of macromolecules through a combination of diffusion, electrophoresis and electro-osmosis. Skin electroporation with [...]

Decoherence suppression in a resonant driving field

Resonant radio frequency (rf) control fields have been employed to suppress decoherence in single quantum bits (qubits) encoded in the probability amplitudes of np fine-structure states in Li Rydberg atoms. As described previously [1], static electric-field tuning of the spin and orbital angular momentum composition of the fine-structure eigenstates enables qubit storage in an approximate decoherence-free subspace in which phase errors due to small stray electric and magnetic fields are strongly suppressed. In addition, it was found that sequences of short electric field pulses could be utilized in a ‘bang-bang’ dynamic decoupling scheme to improve coherence times. We now show that a continuous resonant rf field can also suppress decoherence in this system. The rf-dressed fine-structure states form a more robust basis in which the energy splitting between the component qubit levels is locked to the drive frequency, and decoherence is essentially eliminated. Measurements of the operational range of rf frequency and field strength required to achieve decoherence suppression are in agreement with the predictions of a two-level model.

Resonant properties in the paddlefish electrosensory system caused by delayed feedback

The paddlefish electrosensory system consists of receptor cells in the skin that sense minute electric fields from their prey, small water fleas. The receptors thereby measure the difference of the voltage at the skin surface against the voltage inside the animal. Due to a high skin impedance, this internal voltage is considered to be relatively fixed. We found, however, that this internal voltage can fluctuate. It shows damped oscillations to a single short electric field pulse and changes, with some time delay, according to the previous history of stimulation, and shows resonance at a certain frequency. Computer simulations show that these phenomena can be explained by the presence of delayed feedback where the internal voltage is part of the feedback loop.

Dynamics of the molecular orientation field coupled to ions in two-dimensional ferroelectric liquid crystals

Molecular orientation fluctuations in ferroelectric smectic liquid crystals produce space charges, due to the divergence of the spontaneous polarization. These space charges interact with mobile ions, so that one must consider the coupled dynamics of the orientation and ionic degrees of freedom. Previous theory and light scattering experiments on thin free-standing films of ferroelectric liquid crystals have not included this coupling, possibly invalidating their quantitative conclusions. We consider the most important case of very slow ionic dynamics, compared to rapid orientational fluctuations, and focus on the use of a short electric field pulse to quench orientational fluctuations. We find that the resulting change in scattered light intensity must include a term due to the quasistatic ionic configuration, which has previously been ignored. In addition to developing the general theory, we present a simple model to demonstrate the role of this added term.

Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary.

A549 cells were briefly exposed to Thioglo-1, which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50-300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte-filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on the conditions. Longer pulses and a shorter cell-capillary tip distance led to a greater decrease in the cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell-capillary tip distances were more deadly. An optimum condition exists at a cell-capillary tip distance of 3.5-4.5 microm and a pulse duration of 120-150 ms. At these conditions, >90% of the cells are permeabilized and 80-90% survive.