After labelling with rhodaminated α-bungarotoxin, acetylcholine receptors in cutaneous pectoris muscles of normal adult frogs ( Rana temporaria) appear as brightly fluorescent straight bars, usually extending over the whole gutter. Here we investigated first whether receptor bars can undergo changes and secondly whether they would provide a structural correlate for the strength of a junction. Bars of low fluorescence intensity, as well as short or discontinuous receptor bars consisting of two or three segments, suggest plasticity at the receptor/active zone level. In order to elucidate this notion, receptor bars were studied at different seasons which have previously been shown to be associated with structural changes. In two groups of frogs kept under laboratory conditions simulating wintertime and summertime, respectively, the length and number of receptor bars and the amount of discontinuous bars were investigated. Synaptic contact length, which is the summed length of labelled synaptic branches, and the number and total length of receptor bars did not differ significantly. A clear difference between Group I (“winter” frogs) and Group II (“summer” frogs) was found in the number of discontinuous bars, which was almost twice as high in Group I compared with Group II (6.4 ± 3.3% S.D. vs 3.4 ± 1.3% S.D., n = 8 and 7 muscles, respectively, P < 0.05). In addition, the average length of individual bars was slightly longer in Group I frogs (2.16 ± 0.7 gmm S.D. vs 2.07 ± 0.12 /gmm S.D.,0.1 < P < 0.05). Transmitter release has been shown to be different in these two groups—as determined from endplate potential measurements in tubocurarine-containing bathing solutions—although it was equal when measured in low Ca 2+/high Mg 2+ [Dorlöchter M. et al. (1991) Pflügers Arch. 418, Suppl. 1, R31]. We also investigated whether receptor bars would be a reasonable structural correlate of synaptic function by comparing different measures of transmitter release with different structural parameters in 19 identified junctions. The mean quantal content) m) of a junction was positively correlated with the number and total length of receptor bars, but not with synaptic contact area or length. Amplitudes of the first, maximum, and plateau endplate potentials (corrected for a common resting potential and apparent input resistance) at tetanic nerve stimulation (40 Hz for 2 s) in tubocurarine block were strongly correlated with both synaptic contact length and total receptor bar length ( r = 0.90 for maximum endplate potential); correlations between m and any structural measure were significantly worse. In junctions with a small first endplate potential, facilitation, expressed as the ratio of the maximum/first endplate potential, was higher and the maximum endplate potential was reached later in the train than in those with a large first endplate potential. In contrast, depression was less pronounced or absent in junctions with a small first endplate potential; the ratio of plateau to first endplate potential was larger, and the decline of endplate potential amplitude to below the value of the first endplate potential occurred later in the train or did not occur within 80 impulses. Thus, plateau endplate potentials were more similar among junctions than the first endplate potential; the coefficient of variation was 0.48 for plateau and 0.58 for first endplate potentials. We concluded that fluorescent acetylcholine receptor bars undergo redistribution in the course of environmental changes. Furthermore, their close correlation, especially with the maximum endplate potential in a train, makes them excellent parameters for comparisons of structure and function. The considerable variability in transmitter release among junctions is counterbalanced to some degree by the processes of facilitation and depression.
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