Shifts In Landscape Research Articles

Evolving National Identity in Korea (2012–2021) and Implications for Social Sustainability

National identity in Korea has undergone significant changes in recent years, reflecting broader shifts in social and political landscapes. Traditionally rooted in ethnocultural and ethnic frameworks, the concept of national identity is increasingly influenced by civic and pluralistic values, reflecting Korea’s growing diversity and globalization. This study aims to explore the transformation of national identity in Korea between 2012 and 2021 and its implications for social sustainability, with particular attention paid to Sustainable Development Goal 16. Using latent class analysis, this research examines survey data collected from Korean adults over the specified period. The analysis reveals a decline in ethnocultural and ethnic identities, with a corresponding rise in civic-oriented and flexible identity groups, such as Non-Territorial Ethnocultural Civic, Pluralistic, and Territorial-Cultural groups. These new categories illustrate a more complex understanding of national belonging, shaped by multiple dimensions. The results suggest that this evolving identity landscape promotes greater inclusivity and adaptability, crucial for fostering long-term social cohesion in a diverse society. The study highlights the need for policies, educational reforms, and media narratives that embrace this dynamic understanding of national identity. The findings offer important insights for policymakers and educators as Korea continues to navigate the challenges of diversity and globalization, ensuring a sustainable social future.

Systemic Therapy for Atopic Dermatitis in Children and Adolescents: A US Expert Consensus.

Atopic dermatitis (AD) is a chronic, type-2 mediated, inflammatory skin disease characterized by intense pruritus, disruption of skin barrier function, and immune dysregulation. Management strategies for AD are routinely determined based on disease severity. First-line treatment begins with basic skin care and topical anti-inflammatory medication, which is typically sufficient for the management of mild-to-moderate disease. For those patients with moderate-to-severe disease, systemic therapy is often required. This can involve off-label treatment with conventional immunosuppressant medications. However, this approach is limited by a lack of robust clinical trial data and safety concerns that necessitate close monitoring. The emergence of novel targeted biologics and small molecules to treat AD presents an opportunity to optimize AD management and patient outcomes by offering greater efficacy than traditional immunosuppressants and a favorable safety profile. As the treatment landscape shifts, clinicians can benefit from a standardized process of patient assessment and treatment, along with resources to help maintain contemporary knowledge of available therapeutic options. This US-based, expert-led consensus used a modified Delphi process to develop core recommendations for the use of systemic medications for the management of pediatric patients <18 years of age with moderate-to-severe AD.

Unveiling Kerala’s Distinct Urbanisation: A Comparative Analysis within India

Urbanisation is a pivotal demographic transformation that characterised the twentieth century, serving as a crucial indicator of modernisation intimately intertwined with economic growth and development. Significantly, in its journey as a developing nation, India is witnessing a rapid surge in urbanisation, a trend that surpassed expectations, as evidenced by the 2011 Census. The surge in India’s urban population is primarily attributed to a combination of net rural–urban reclassification and rural-to-urban migration. It is essential to recognise the distinctive nature of Kerala in this context, as the state diverges significantly from the patterns observed in other regions. The urbanisation unfolding in Kerala is not confined solely to designated towns and cities; instead, the entire state portrays a nuanced urban–rural continuum. In this study, an exploration is undertaken to dissect the trends and patterns of urbanisation in Kerala, delving into its unique characteristics and associated challenges based on data provided by the Census of India. Regression analysis is used to determine the factors that contribute to Kerala’s urbanisation, which shows that urbanisation process is influenced by a complex interplay of financial, economic and demographic factors. Over the decade, banking has become a crucial driver of urbanisation, highlighting its growing role in supporting urban growth and development. In contrast, the significance of investment in small-scale industries/micro, small and medium enterprises has diminished by 2011, suggesting shifts in the economic landscape and pointing to the rising importance of services and remittances as sources of urban expansion. Household dynamics, consistently significant in both years, emphasise the critical role of population changes and family structures in shaping urbanisation trends. Despite the continuous rise in urbanisation rates, there persists a noticeable sluggishness in development, especially regarding the provision of essential amenities. This aspect necessitates focused policy attention to address the challenges accompanying Kerala’s evolving urban landscape. JEL Codes: O18, R23, R12

Preparing Physician-Scientists for the Future of Academic Medicine.

The changing field of academic medicine presents unique challenges for physician-scientists, who intricately weave the complexities of research and patient care. These challenges have significantly lengthened the time needed for scientific discoveries to be applied in clinical practice. In response to these escalating demands, the training trajectory for physician-scientists has notably expanded over recent decades. In anticipation of and preparation for future training requirements, the National Pediatrician-Scientist Collaborative Workgroup facilitated a series of convenings with a diverse array of stakeholders vested in physician-scientist training. The framework Strategic Foresight was used to explore possible future scenarios and develop strategic plans. Seven pivotal themes, termed "performance zones," emerged from this endeavor: (1) revolution in education through technology-enhanced learning, (2) educational outcomes beyond content mastery, (3) artificial intelligence-empowered research portfolios and expansive networks, (4) evolution of high-performance inclusive, equitable, distributed, and agile teams, (5) evolution of antifragile systems, (6) a multiparadigmatic program of research, and (7) interdependence and commitments to a common agenda for collective impact. These identified zones underscore the imperative for physician-scientists to acquire novel skill sets essential for navigating the impending shifts in the health care landscape. These findings are poised to steer policy initiatives and educational advancements, fortifying the foundation for future physician-scientist training needs.

Lessons from the Pilot Project of Korean ETS on the Local Landscape of Economy

For a sustainable landscape of local economies, many researchers have emphasized the importance of field-oriented differentiation in government policies. In particular, the Paris Agreement, based on the bottom-up approach, aims to maximize the participation of all economic agents, in contrast to the top-down approach of the Tokyo Protocol. In response to these global paradigm shifts in the local landscape, local governments in Korea have made significant efforts to adapt to sustainable development during the pilot phase of emission trading scheme (ETS), during the period from 2015 to 2020. This study evaluates the performance of these local government policies in the transition to a carbon-zero economy. Using the general non-radial directional distance function (GNDDF), we found that Gyeongsang Province demonstrated enhanced environmental total factor productivity (TFP) during the pilot project, whereas the Seoul metropolitan area lagged behind due to a lack of governance. As the economic center of Korea, Seoul showed poor environmental performance because of the arbitrary elimination of green belt areas and unchecked land development, resulting in environmental degradation, a trend common in many developing countries facing climate adaptation challenges. To address these urbanization issues, this study concludes that a balanced approach combining stricter regulations with market-oriented promotional incentives is essential for optimizing the transition of local economies to a sustainable landscape.

The emergence of multipolarity in global higher education: the Belt and Road Initiative and African students’ motivations to pursue postgraduate education in China

ABSTRACT The past decade has witnessed an unprecedented transformation of China into an important “receiving” nation for international student mobility (ISM), underscored by the Belt and Road Initiative (BRI)-tied African countries becoming the main import markets. The emerging China-bound ISM, driven by contextually-specific motivations, necessitates a re-theorisation of the Western-centric, neo-liberal-driven push–pull model within the broader context of multipolarity in higher education. Through semi-structured in-depth interviews with eight international students from BRI African countries pursuing postgraduate programmes in Beijing, this micro study explores the dynamic macro–micro interface of push and pull factors. These narratives of how the push (e.g. the under-developed higher education system in the home country) and pull factors (i.e. host nation’s financial support for international students, economic and political links between the home and host countries, and host nation’s exponential growth) intersected with individuals’ desire, reflect the multipolar shifts in global higher education landscapes. By extending the macro-focused push–pull model to incorporate these complexities, this study echoes the need to recognise contemporary multipolarity when explaining ISM in diverse contexts.

Adaptive strategies and sustainable investments: navigating organizations through a VUCA environment in and after COVID-19

This study delves into the resilience and adaptability of employees within the volatile, uncertain, complex, and ambiguous (VUCA) business environment, examining their readiness to manage effectively and the organizational agility in navigating change, alongside the impact of sustainable investment practices. Employing quantitative methods, a survey was conducted among employees at two pivotal moments: during and after the COVID-19 pandemic restrictions. Factorial analysis revealed a strong preference for participatory work styles and highlighted the critical need for employee involvement in significant decision-making processes. Although the value of sustainable investments was recognized, a noticeable gap was found in employees’ understanding and adaptability towards these investments. The use of the Wilcoxon test illuminated the significant impact of external disruptions, such as the pandemic, on organizational operations and preparedness. The findings underscore the imperative for organizations to champion continuous learning and training, enabling strategic and innovative responses to the challenges unique to the VUCA world. By aligning adaptive interventions with the demands of the VUCA environment, organizations can define a clear trajectory towards sustainable growth and enhance their proactive stance against sudden shifts in the business landscape.

A game of snakes and ladders: the world of complex health and social care data linkage

Objective and ApproachResearch on social care services requires large, comprehensive, routine datasets. Launched in November 2019, Developing resources And minimum data set for Care Homes’ Adoption (DACHA) study aims to develop a prototype minimum dataset as proof of concept and to propose implementation. We describe our experience since 2021 of identifying, applying for, and linking care home, local, integrated care system (ICS) and national datasets, including direct-care software-provider data, GP and NHS England (NHSE) datasets. ResultsOur key challenges: Risk aversion and fragmented information governance within and between organisations. Complex information governance structures must be understood before participants are recruited. Shifts in the data landscape: data ownership moved from clinical commissioning groups to ICSs; NHSE merged with NHS Digital. Research funding: research is required of ICSs, but it is not a system priority, so processes and funding aren’t in place, and research burdens an already strained system. Our key lessons: Formalising agreements early on and obtaining, and maintaining, senior, committed buy-in. Studies should value the data sharing process through the inclusion of data controllers and information governance staff in the system in research project budgets. The facilitation of GP and social care data collection nationally must be balanced with its administrative burden. ImplicationsThe DACHA study overlapped with the COVID-19 pandemic and could only engage a limited number (n=3) of ICSs within project resources. Nevertheless, the complexity of the system and the plurality of actors delay or even block legitimate research interests.

Reviewing Demand Response for Energy Management with Consideration of Renewable Energy Sources and Electric Vehicles

This review paper critically examines the role of demand response (DR) in energy management, considering the increasing integration of renewable energy sources (RESs) and the rise in electric vehicle (EV) adoption. As the energy landscape shifts toward sustainability, recognizing the synergies and challenges offered by RESs and EVs becomes critical. The study begins by explaining the notion of demand response, emphasizing its importance in optimizing energy usage and grid stability. It then investigates the specific characteristics and possible benefits of incorporating RESs and EVs into DR schemes. This assessment evaluates the effectiveness of DR techniques in leveraging the variability of renewable energy generation and managing the charging patterns of electric vehicles. Furthermore, it outlines important technological, regulatory, and behavioral impediments to DR’s mainstream adoption alongside RESs and EVs. By synthesizing current research findings, this paper provides insights into opportunities for enhancing energy efficiency, lowering greenhouse gas emissions, and advancing sustainable energy systems through the coordinated implementation of demand response, renewable energy sources, and electric vehicles.

Unexpected hydrologic response to ecosystem state change in tallgrass prairie

In grasslands around the world, climate change is occurring in tandem with woody encroachment (the spread of woody vegetation in grass-dominated ecosystems). State transitions from grassland to shrub/woodland have been identified aboveground via changes in species cover and composition, but the hydrological impact of these transitions is not well understood. Shifts from grass- to woody-dominance have the potential to impact evapotranspiration, soil moisture dynamics, and groundwater recharge. Therefore, it is possible that the consequences of aboveground vegetation change may be observable in the hydrological system. We leveraged long-term hydrological records from a tallgrass prairie site in northeastern Kansas, USA, to examine how concurrent changes in climate and land-cover have altered hydrological dynamics over the last century. Stream discharge has declined in recent decades despite > 100 years of climate wetting. The relationship between incoming precipitation and streamflow has weakened over the last 40 years, suggesting that shifts in the physical landscape are altering patterns of hydrological connectivity. Long-term isotope records show a divergence in the isotopic composition of precipitation (no change in δ18O) and stream/groundwater (decreasing δ18O) over the last decade. These results suggest that woody encroachment is accelerating the hydrological cycle, potentially by decreasing groundwater recharge (via increased evapotranspiration) and/or increasing infiltration rates (via creation of macropores). Holistically, these changes illustrate the interdependence of above- and below-ground processes in the local hydrological cycle, and the cascading long-term consequences (decades to centuries) for critical zone function once woody encroachment has occurred.

A Comparative Study To Assess The Attitude Of Working Men And Women (Age 20-40 Years) Towards Marriage In Delhi NCR

Marriage is a cornerstone of society, playing a significant role in shaping individuals' lives and contributing to the fabric of communities. Over the past few decades, profound shifts in societal norms, cultural values, and economic landscapes have reshaped perceptions and attitudes towards marriage, particularly among working individuals, whose professional lives often intersect with their personal aspirations and relationship dynamics. For Nursing Personnel, understanding marital status is important because of its influence on psychological and physical well-being. A comparative study was undertaken to assess the Attitude of working Men and Women (Age 20-40 years) towards Marriage in Delhi NCR. A Comparative survey design was adopted for the study. The data was collected, using a 7-point Likert Scale via Google Forms. 100 men and 100 women were selected from MNC, School, Hospital and Society of Delhi NCR. A standardized tool, “The Marital Scale” (Park and Rosen) was used. The data was tabulated and analyzed using descriptive and inferential statistics. The findings of the study showed that a predominance of respondents in the 26-30 age, with a considerable educational attainment of graduates (46%) and postgraduates (41%). Hindu (54%) and Christian (36%) affiliations were most common, with nuclear families (48%) prevailing over joint ones (33%). In the private sector, 64% were employed, with a majority (61%) earning below Rs. 50,000 monthly. Preference for partners of the same caste or religion was evident in 59% of respondents. The study revealed that men have more positive attitude towards marriage as compared to women. Men displayed a slightly higher mean attitude score (174.8) than women (167.31), with a significant mean difference of 7.56. However, no significant association was found between attitudes towards marriage and demographic variables

Late-glacial and Holocene shifts in the mountain landscapes of the Cantabrian range (northern Spain) in response to changing climate, fire occurrence and land use

A new well-dated (18 14C dates on short-lived terrestrial plant macrofossils, 210Pb and 137Cs dating) fine-resolution palaeoecological sequence (pollen, coprophilous fungi, conifer stomata, macrofossils, charcoal) from Lago de Isoba (1400 m a.s.l.) spanning the past c. 17,000 years provides novel insights into the long-term dynamics of the high-elevation Cantabrian landscape in response to climate variability and human activities. Vegetation changes were mainly climate-driven during the Late-glacial and the Early Holocene. Thus, steppe-like environments prevailed during the Greenland Stadial 2.1a (GS-2.1a ≈ ‘Oldest Dryas’). Warmer and moister conditions during the Greenland Interstadial 1 (GI-1 ≈ ’Bølling-Allerød’) enabled successive expansions of Juniperus, Betula and Pinus. Steppe-like vegetation re-expanded during the cooler and drier Greenland Stadial 1 (GS-1 ≈ ‘Younger Dryas’). Afterwards, the Early Holocene warming triggered the rapid spread of mixed pine-broad-leaved deciduous mountain forests. Pollen, stomata and macrofossils indicate continuous, long-lasting (c. 10,850–1250 cal yr BP) local occurrence of Pinus sylvestris stands. The records of Cerealia and Plantago lanceolata pollen types (first Holocene occurrences dated at c. 5100 cal yr BP) evidence increased land use during the Late Holocene, which triggered substantial changes in forest ecosystems. Following a phase of intensified arable and pastoral farming, which involved the use of fire to create and maintain forest clearings, pinewoods expanded and reached their maximum development between c. 3600 and 3300 cal yr BP. Pinus probably outcompeted temporarily other woody taxa thanks to its resilience to low-intensity disturbances. Later, pinewoods underwent a stepwise, human-induced decline ending in their final massive collapse after c. 1150 cal yr BP. The highest intensity of agricultural and pastoral farming took place during the Middle Ages, following maximum fire occurrence. We conclude that the present-day open landscape has been shaped by millennia of land use that partly blurred the impact of climate variability.

Application of metal oxide/porous carbon nanocomposites in electrochemical capacitors: A review

In the past decade, electrochemical capacitors (ECs) have emerged as innovative energy storage devices, attracting considerable interest from both industrial and academic sectors due to their prolonged cyclic stability and impressive power density. Recently, there has been a focus on advancing high-performance ECs by developing electrode material composed of metal oxide incorporated with porous carbon. Integrating metal oxides into a porous carbon structure enhances capacitance, charge storage, and cyclic stability. Furthermore, the porous structure inherent in carbon materials offers efficient ion diffusion and large surface area, thereby augmenting the overall effectiveness of the nanocomposites. Efficient electron and ion transport within the electrode's bulk is achieved through the interaction between electrodes and electrolytes occurring at the electrode-electrolyte interface, subsequently increasing the capacitance. This paper summarizes the latest developments in ECs, focusing on key performance metrics such as cyclic stability and capacitance. It also summarizes the recent advancements made in electrode materials, emphasizing the utilization of sustainable feedstocks. As the research landscape shifts towards more environmentally friendly solutions, the incorporation bio-materials into electrode design has gained significant attention. It particularly highlights underlying mechanisms governing the performance of metal oxides/porous carbon nanocomposites in ECs and outlines future research directions aimed at maximizing their potential for next-generation energy storage devices. Additionally, the paper delves into the possibilities and challenges associated with the preparation, and optimization of the structure of metal oxide electrodes incorporating porous carbon to improve energy storage performance in ECs.

Interrogating Internal Temperature Evolution in Li-Ion Cells

Li-ion batteries have become indispensable today as the energy landscape shifts towards more sustainable options through electrification. A critical aspect of enhancing their cycle life while maintaining safety and performance lies in the effective thermal management of the battery. Currently, most battery management systems (BMS) rely on external temperature sensors to estimate internal temperatures. However, rapid internal heat generation can lead to a significant disparity between external and internal temperatures, particularly during extreme operating conditions. This study delves into operando monitoring of internal temperatures during cycling of Li-ion cells at various operating temperatures and C-rates. This offers insights into the evolution of internal temperatures throughout cycling and the difference between external and internal temperatures. This information can be instrumental in developing computational models that can predict internal temperatures more accurately using non-invasive techniques in the future.

Migration and erosion in tidal and river channels in Bangladesh

Environmental stressors and natural disasters are changing the physical landscapes in many countries worldwide. In this paper, we ask whether and how erosion affects internal and international migration in Bangladesh. Building on prior studies, we use data from 3,600 households in 18 research sites to investigate how erosion in tidal and river channels is related to the risk of making a first internal or international trip, net of extreme weather conditions and other relevant attributes. Findings reveal that the relationship between erosion and the likelihood of making a first domestic or international trip is moderated by livelihood type and landownership. As erosion worsens, the odds ofmaking a first domestic trip rise for non-agricultural non-landowning household heads and decline for landowners working in agriculture. Estimated lifetime probabilities of making a first domestic trip are higher than those of making a first international trip, with non-agricultural non-landowners having the highest probabilities and agricultural landowners having the lowest. Together, the evidence suggests that shifts in physical landscapes, especially erosion, are tightly linked to out-migration through ties to land.

Abstract Tu037: Unraveling the Cellular Mechanisms of Cardiogenic Liver Disease

Background: Right heart failure (RHF) is associated with chronically elevated central venous pressures which can lead to hepatic dysfunction, fibrosis, and ultimately cirrhosis. Despite the prognostic importance of liver disease in RHF little is understood about the cellular and molecular changes that define cardiogenic liver disease. Filling this knowledge gap has the potential to lead to better diagnostic and therapeutic approaches for patients with heart failure and liver dysfunction. Aims: 1) To evaluate the key clinical and pathologic features of cardiogenic liver disease in humans 2) To explore shifts in the cellular landscape in the congested liver using human samples and a mouse model congestive hepatopathy. Methods: We identified 34 patients with heart failure who underwent liver biopsy due to concern for cardiogenic liver disease. These biopsy samples were stained for numerous cellular markers (macrophage subsets, activated stellate cells, endothelial cells, biliary cells) and compared to normal controls. Clinical data was also collected for the included patients. To model cardiogenic liver disease in a mouse we performed partial IVC ligation (pIVCL) to induce liver congestion and then assessed the liver response using flow cytometry, tissue imaging and mRNA analysis. Results: Liver biopsies from patients with cardiogenic liver disease demonstrated increased liver fibrosis, macrophage aggregates, and biliary metaplasia. The severity of biliary metaplasia was closely associated with the extent of liver congestion. Using multi-marker staining the metaplastic cells were found to be of hepatocyte origin. Interestingly, the liver niche containing metaplastic biliary cells was also enriched for recruited macrophages and activated stellate cells. Similar pathologic features were observed in the mouse model of liver congestion. Conclusions: In this study we identified biliary metaplasia and macrophage recruitment as key pathologic features of cardiogenic liver disease. Ongoing investigation of the interplay between macrophages and metaplastic biliary in humans and animal models of congestive hepatopathy may lead to the discovery of novel disease mechanisms and biomarkers for liver dysfunction in heart failure.

Iran’s Foreign Policy from Non-Alignment to ‘Look to the East’: Between Ideology and Pragmatism

In the wake of the 1979 Revolution, the newly established Islamic Republic of Iran aimed to chart an autonomous foreign policy distinct from the Cold War’s binary dynamics, proclaiming a stance of ‘neither East, nor West’. From the outset, this non-alignment aspiration exposed the many diverse interpretations of this political concept, which served to accentuate Iranian particularism and factionalism. Despite the Islamic Republic’s commitment to the principles of non-alignment over decades, shifts in the global landscape and domestic economic imperatives have gradually pushed Tehran toward more conciliatory approaches with nations beyond the Non-Aligned Movement (NAM), leading it to increasingly align itself with ‘the East’, particularly Russia and China. Since the latter half of the 1980s, Tehran has actively sought to bolster its foreign relations with countries beyond the NAM, culminating in recent developments such as the 2015 multilateral agreement on Iran’s nuclear program and the forging of the respective 20-year cooperation pacts with Russia and China. This article explores the transformation of Iranian non-alignment, examining the various political and economic factors that have compelled the Islamic Republic to uphold its revolutionary ideology of self-determination and independence, while at the same time pursuing more pragmatic policies seemingly at odds with the principles of non-alignment.

Aging-associated Alterations in the Gene Regulatory Network Landscape Associate with Risk, Prognosis and Response to Therapy in Lung Adenocarcinoma.

Aging is the primary risk factor for many individual cancer types, including lung adenocarcinoma (LUAD). To understand how aging-related alterations in the regulation of key cellular processes might affect LUAD risk and survival outcomes, we built individual (person)-specific gene regulatory networks integrating gene expression, transcription factor protein-protein interaction, and sequence motif data, using PANDA/LIONESS algorithms, for both non-cancerous lung tissue samples from the Genotype Tissue Expression (GTEx) project and LUAD samples from The Cancer Genome Atlas (TCGA). In GTEx, we found that pathways involved in cell proliferation and immune response are increasingly targeted by regulatory transcription factors with age; these aging-associated alterations are accelerated by tobacco smoking and resemble oncogenic shifts in the regulatory landscape observed in LUAD and suggests that dysregulation of aging pathways might be associated with an increased risk of LUAD. Comparing normal adjacent samples from individuals with LUAD with healthy lung tissue samples from those without LUAD, we found that aging-associated genes show greater aging-biased targeting patterns in younger individuals with LUAD compared to their healthy counterparts of similar age, a pattern suggestive of age acceleration. This implies that an accelerated aging process may be responsible for tumor incidence in younger individuals. Using drug repurposing tool CLUEreg, we found small molecule drugs with potential geroprotective effects that may alter the accelerating aging profiles we found. We also observed that, in contrast to chronological age, a network-informed aging signature was associated with survival and response to chemotherapy in LUAD.

Genome-wide methylation landscape during somatic embryogenesis in Medicago truncatula reveals correlation between Tnt1 retrotransposition and hyperactive methylation regions.

Medicago truncatula is a model legume for fundamental research on legume biology and symbiotic nitrogen fixation. Tnt1, a retrotransposon from tobacco, was used to generate insertion mutants in M. truncatula R108. Approximately 21 000 insertion lines have been generated and publicly available. Tnt1 retro-transposition event occurs during somatic embryogenesis (SE), a pivotal process that triggers massive methylation changes. We studied the SE of M. truncatula R108 using leaf explants and explored the dynamic shifts in the methylation landscape from leaf explants to callus formation and finally embryogenesis. Higher cytosine methylation in all three contexts of CG, CHG, and CHH patterns was observed during SE compared to the controls. Higher methylation patterns were observed in assumed promoter regions (~2-kb upstream regions of transcription start site) of the genes, while lowest was recorded in the untranslated regions. Differentially methylated promoter region analysis showed a higher CHH methylation in embryogenesis tissue samples when compared to CG and CHG methylation. Strong correlation (89.71%) was identified between the differentially methylated regions (DMRs) and the site of Tnt1 insertions in M.truncatula R108 and stronger hypermethylation of genes correlated with higher number of Tnt1 insertions in all contexts of CG, CHG, and CHH methylation. Gene ontology enrichment and KEGG pathway enrichment analysis identified genes and pathways enriched in the signal peptide processing, ATP hydrolysis, RNA polymerase activity, transport, secondary metabolites, and nitrogen metabolism pathways. Combined gene expression analysis and methylation profiling showed an inverse relationship between methylation in the DMRs (regions spanning genes) and the expression of genes. Our results show that a dynamic shift in methylation happens during the SE process in the context of CG, CHH and CHG methylation, and the Tnt1 retrotransposition correlates with the hyperactive methylation regions.

Corporate governance and firm performance: Evidence from political instability, political ideology, and corporate governance reforms in Pakistan

AbstractWe examine impact of corporate governance on firm performance following the implementation of the changes to the Code of Corporate Governance of Pakistan in 2012. Our sample period from 2008 to 2022 include periods of political instability and shifts in Pakistan's political landscape, providing an opportunity to examine the effectiveness of corporate governance mechanisms in enhancing accounting‐ and market‐based firm performance measures. We find significant improvements attributed to reforms in the regulatory framework surrounding corporate governance practices particularly from expanded scope and composition of boards and audit committees. This led to broader capabilities and effective controls, thus improving firm performance and investor confidence more so during periods of political instability and changes in political ideology.