Shelter Grounds Research Articles

Molecular Evidence for Transstadial Transmission of Ehrlichia canis by Rhipicephalus sanguineus sensu lato Under Field Conditions.

This study investigated possible transstadial transmission of Ehrlichia canis by Rhipicephalus sanguineus sensu lato collected from shelter dogs and the shelter grounds in Diyarbakır Province of south-eastern Turkey. Totally 225 engorged nymphs were collected from eight infected dogs with E. canis and incubated at 28°C for moulting. Unfed ticks from the shelter grounds comprising 1,800 larvae, 3,100 nymphs, and 85 adults were sorted according to sampling origin, life stage, and sex into 116 pools and screened by 16S rRNA PCR. Nine out of 26 pools of unfed adult ticks were positive for E. canis, with overall infection rate maximum likelihood estimation (MLE) of 4.83 (CI 2.39-8.87). E. canis was detected in three of 12 male pools (MLE 3.22, CI 0.86-8.83) and six of 14 female pools (MLE 6.16, CI 2.59-12.90). No adult pools collected from the shelter grounds were positive. Among 62 unfed nymph pools collected from the shelter, six were infected with E. canis (MLE 0.20, CI 0.08-0.42). No E. canis DNA was detected in any of the larva pools. Our results revealed molecular evidence for transstadial transmission of E. canis by R. sanguineus s.l. both from larva to nymph and from nymph to adult. We found no evidence of transovarial transmission.

Molecular evidence for trans-stadial transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato under field conditions.

This study investigated the transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae). Engorged nymphs (n = 404) removed from A. platys-infected dogs were incubated at 28 °C until moulting. Unfed adults were obtained and divided into 48 pools. Unfed ticks collected from the grounds of the dog shelter, comprising 1800 larvae (n = 18 pools), 3100 nymphs (n = 62 pools) and 85 adults (n = 10 pools, including three male and seven female pools) were sorted into 90 pools. All pools were screened by polymerase chain reaction for the 16S rRNA gene of A. platys. Of 48 pools of unfed adults obtained from engorged nymphs, 12 were positive for A. platys; the infection rate maximum likelihood estimate (MLE) was 3.36 [95% confidence interval (CI) 1.84-5.68]. Anaplasma platys was detected in five of 23 male pools (MLE 2.82, 95% CI 1.06-6.20) and seven of 25 female pools (MLE 3.83, 95% CI 1.72-7.57). Of seven pools of unfed adult females collected from the shelter grounds, one was positive for A. platys (MLE 1.74, 95% CI 0.11-8.22). Among 62 unfed nymph pools, eight were infected with A. platys (MLE 0.27, 95% CI 0.13-0.52). No A. platys DNA was detected in the larva pools. The present results reveal molecular evidence for the trans-stadial transmission of A. platys by R. sanguineus s.l.

The Tribal Economy in Western Madhya Pradesh: Environmental Perspectives and Changes in Livelihood Patterns

Economy is the base of all activities in human life. It provides food which is the foremost important need of any living organism on earth. Food and related activities to acquire food vary from region to region and community to community. Hence, tribals form the hunters, gatherers, primitive agriculturists and so many other categories on the basis of their economy. Forests have remained the usual abode of the tribals in most parts of the world, particularly in India. Hilly tracts with the forest covers have become permanent shelter grounds for them due to obvious and non-apparent politico-economic reasons. Forest provides a suitable hunting ground and definitely a gatherers’ paradise for most of the hunting and food-gathering tribes of India. It is the call of the time to have sensible exploitation of the forest resources along with substantial forestation in the same area for keeping the balance of means and consumption in the forest. About a couple of decades ago the population was less and the forests were plenteous in quantity and variety both. Now, the situation is almost reverse. Forest reserves have gone down, while the population has increasingly gone up. The tribals are still the greatest friends of the forests and coexistence of both is essential. The Bhilala and the Patelia of some areas of Western Madhya Pradesh are deemed to be the good cultivators in the region due to their hard work, adoption of new agricultural techniques and use of fertilisers.