Sheep Research Articles

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries).

Pigment production and distribution are controlled through multiple proteins, resulting in different coat color phenotypes of sheep. The expression distribution of vimentin (VIM) and transthyretin (TTR) in white and black sheep skins was detected by liquid chromatography-electrospray ionization tandem MS (LC-ESI-MS/MS), gene ontology (GO) statistics, immunohistochemistry, Western blot, and quantitative real time polymerase chain reaction (qRT-PCR) to evaluate their role in the coat color formation of sheep. LC-ESI-MS/MS results showed VIM and TTR proteins in white and black skin tissues of sheep. Meanwhile, GO functional annotation analysis suggested that VIM and TTR proteins were mainly concentrated in cellular components and biological process, respectively. Further research confirmed that VIM and TTR proteins were expressed at significantly higher levels in black sheep skins than in white sheep skins by Western blot, respectively. Immunohistochemistry notably detected VIM and TTR in hair follicle, dermal papilla, and outer root sheath of white and black sheep skins. qRT-PCR results also revealed that the expression of VIM and TTR mRNAs was higher in black sheep skins than in white sheep skins. The expression of VIM and TTR were higher in black sheep skins than in white sheep skins and the transcription and translation were unanimous in this study. VIM and TTR proteins were expressed in hair follicles of white and black sheep skins. These results suggested that VIM and TTR were involved in the coat color formation of sheep.

Domestication and Genetic Diversity Analysis of Several Sheep Breeds in China

Sheep is the main livestock species in northern China. Under diverse climatic conditions, a gene pool of local sheep resources is now forming. There are many sheep breeds in northern China, including local breeds and hybrid breeds. However, little is known about the genetic background and genetic variation of local sheep populations in northern China. Therefore, our aims is to provide a characterization of the population structure and genetic relationship within sheep breeds in northern China, and to study its genetic relationship with other sheep breeds. We collected blood samples from 390 sheep for genetic diversity analysis using Reduced-Representation Genome Sequencing (RRGS). This study used bioinformatics methods to analyze linkage disequilibrium, population structure, evolutionary tree, selective sweep etc. The mixed model revealed in this study may be formed in the process of sheep breeding, partially overlapping with historical and archaeological findings. New information on the domestication and genetic variety of sheep is provided by this study, which will help sheep in the future be improved through genomics.

Cardioprotective properties of quercetin in fescue toxicosis-induced cardiotoxicity via heart-gut axis in lambs (Ovis Aries)

The present study investigated whether quercetin mitigated fescue toxicosis-induced cardiovascular injury via the heart-gut axis. Twenty-four commercial Dorper lambs were stratified by body weight and assigned randomly to diets in one of four groups: endophyte-free without quercetin (E-,Q-), endophyte-positive without quercetin (E+,Q-), endophyte-positive plus 4 g/kg quercetin (E+,Q+) or endophyte-free plus 4 g/kg quercetin (E-,Q+) for 42 days. Body weight and average daily feed intake (ADFI) of lambs fed the endophyte-positive diets showed significant decreases. However, in the groups treated with quercetin, there were significant alterations of cardiac enzymes. Furthermore, reduced fescue toxicosis-induced histopathological lesions of heart and aorta were demonstrated in the E+,Q+ lambs. Results also suggested quercetin eased cardiovascular oxidative injury by inhibiting the increase of oxidative metabolites, and enhancing the levels of antioxidases. Quercetin reduced the inflammation response through suppressing NF-κB signaling pathway activation. Additionally, quercetin ameliorated fescue toxicosis-induced mitochondria dysfunction and improved mitochondrial quality control through enhancing PGC-1α-mediated mitochondrial biogenesis, maintaining the mitochondrial dynamics, and relieving aberrant Parkin/PINK-mediated mitophagy. Quercetin enhanced gastrointestinal microbial alpha and beta diversity, alleviated gut microbiota and microbiome derived metabolites-SCFAs dysbiosis by fescue toxicosis. These findings signified that quercetin may play a cardio-protective role via regulating the heart–gut microbiome axis.

The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes.

The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.

Comprehensive investigation of milk oligosaccharides in different mammalian species and the effect of breed and lactation period on sheep milk oligosaccharides

Milk oligosaccharides (MOs) have unique health benefits for newborns, and MOs are important components in mammalian milk. The present study was conducted to provide a comprehensive analysis of MOs in important domestic animals, including goats, cows, camels and sheep. The comparison with human MOs was conducted simultaneously. Furthermore, analysis of the relative abundance of sheep MOs among different breeds (Hu sheep, East Friesen sheep, East Friesen-Hu crossbred sheep) and lactation periods (colostrum, mature milk) was performed. In general, 35, 24 19, 26, and 16 MOs were identified in human, goat, bovine, camel and sheep milk, respectively. The type of sheep MOs was not greatly influenced by the breeds and lactation period. Hu sheep colostrum had the highest abundance of MOs among six sheep milks, followed by East Friesen sheep colostrum, while East Friesen-Hu crossbred sheep mature milk had the lowest abundance of MOs. These findings provide evidence for the potential value of MOs from domestic animal milk for the commercial applications.

Sustainability of Animal Stocks in Traditional Pastoral Systems of the High-Altitude Andean Altiplano

The main traditional livelihood in the high Central Andes Plateau, locally known as the Puna, is pastoralism. This system is based on llamas (Lama glama) and sheep (Ovis aries), which graze on natural vegetation (shrub steppe, grasslands, and peatlands). So, the livelihood of Andean communities is strongly influenced by the spatial and temporal dynamics of vegetation. Most of the existing models on herbivory used to estimate sustainable livestock loads require information on the nutritional values and primary production of vegetation, which is lacking for the Argentine Puna. This work aimed to fill the gap by implementing a carrying capacity model using data on annual vegetation growth and herbivore feeding strategies collected in the field in different plant communities. The hypothesis that guided this work was that environmental heterogeneity affects management decisions and should be taken into account to model sustainable animal stocks in Andean pastoralist systems. We estimated carrying capacity based on the MSY logistic model, considering biomass and aboveground primary production of vegetation communities, climatic features, and herbivore selective responses. We compared the estimated carrying capacity with real animal stocks in four production units within the study site in 2013, 2014, and 2017. The results indicate that the carrying capacity presents high spatial and temporal variability. In three of the production units assessed, the livestock—mixed herds of sheep and llamas—were above or near the estimated carrying capacity, even when the stocking rates were reduced during dry periods. The high stocking rates, along with evidence of land degradation, may indicate moderate to severe overgrazing. This work demonstrates the usefulness of estimating the carrying capacity to monitor the pastoral systems at fine space and time scales. These monitoring techniques can facilitate decision making among Puna herders in a biocultural context of knowledge dialog that may foster sustainability in pastoralist livelihoods.

Gastro-intestinal Parasites of Sheep (Ovis aries, Linnaeus, 1758) in Laxmipur VDC, Dang, Nepal

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Effect of feeding different levels of forages and concentrate ration on production performance, serum biochemical and hematological profile in Ghaljo sheep (Ovis aries)

Effect of feeding different levels of forages and concentrate ration on production performance, serum biochemical and hematological profile in Ghaljo sheep (Ovis aries)

Neolithic faunal resources in the center of the Iberian Peninsula. New evidence from El Mar (Meco, Spain)

The zooarchaeological evidence available for the Neolithic in the Iberian Peninsula is mostly condensed in the Mediterranean area, from Catalonia to Cádiz, and the Cantabrian area. This leaves an extensive region with little known evidence. The entire central zone of the Iberian Peninsula, specifically the Middle Tagus Valley, is a region for which there is not much information on the fauna uses. The reasons behind this gap are diverse, so any discovery in this region is relevant to expanding the available data. This paper presents the findings of a new archaeological site discovered during the last few years. El Mar, located in Meco (Madrid) offers evidence of cow and sheep domestication. And, thus, joins the existing zooarchaeological record for other nearby sites in the Middle Tagus Valley in the center of the Iberian Peninsula.

Wild sheep on St Kilda.

Veterinary RecordVolume 192, Issue 11 p. 450-452 Animal Welfare Wild sheep on St Kilda First published: 02 June 2023 https://doi.org/10.1002/vetr.3145Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. References 1Jewell PA, Milner C, Morton Boyd J. Island Survivors: The Ecology of the Soay Sheep on St Kilda. London, Athlone, 1974 2Clutton-Brock J, Dennis-Bryan K, Armitage P, et al. Osteology of the Soay sheep. https://bit.ly/42X1hMc (accessed 16 May 2023) 3Kijas JW, Lenstra JA, Hayes B, et al. Genomic analysis of the world's sheep breeds reveals high levels of historic mixture and strong recent selection. PLoS Biol 2012; 10:e1001258 4Chessa B, Pereira F, Arnaud F, et al. Revealing the history of sheep domestication using retrovirus integrations. Science 2009; 324: 532– 6 5 Home Office. Animals (Scientific Procedures) Act 1986. Working with animals taken from the wild. https://tinyurl.com/4pkyd2f4 (accessed 24 May 2023) 6Fleming A. Soay sheep: the back story. Cam Archaeol J 2021; 31: 419– 36 7Harman M. An Isle called Hirte: History and Culture of the St Kildans to 1930. Maclean Press, 1997 8Clutton-Brock TH. The causes and consequences of instability. In: TH Clutton-Brock, JM Pemberton eds. Soay Sheep: Dynamics and Selection in an Island Population. Cambridge University Press, 2004: 304, 307 9Buckland D, Charlesworth G. PE2021: Ensure the definition of protected animals in the Animal Health and Welfare (Scotland) Act 2006 applies to the sheep on St Kilda. https://tinyurl.com/276skns9 (accessed 24 May 2023) Volume192, Issue113/10 June 2023Pages 450-452 ReferencesRelatedInformation

Discrimination of lamb (Ovis aries), emu (Dromaius novaehollandiae), camel (Camelus dromedarius) and beef (Bos taurus) binary mixtures using a portable near infrared instrument combined with chemometrics.

Consumers demand safe and nutritious foods at accessible prices; where issues associated with adulteration, fraud, and provenance have become important aspects to be considered by the modern food industry. There are many analytical techniques and methods available to determine food composition and quality, including food security. Among them, vibrational spectroscopy techniques are at the first line of defence (near and mid infrared spectroscopy, and Raman spectroscopy). In this study, a portable near infrared (NIR) instrument was evaluated to identify different levels of adulteration between binary mixtures of exotic and traditional meat species. Fresh meat cuts of lamb (Ovis aries), emu (Dromaius novaehollandiae), camel (Camelus dromedarius) and beef (Bos taurus) sourced from a commercial abattoir were used to make different binary mixtures (95% %w/w, 90% %w/w, 50% %w/w, 10% %w/w and 5% %w/w) and analysed using a portable NIR instrument. The NIR spectra of the meat mixtures was analysed using principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA). Two isosbestic points corresponding to absorbances at 1028nm and 1224nm were found to be consistent across all the binary mixtures analysed. The coefficient of determination in cross validation (R2) obtained for the determination of the per cent of species in a binary mixture was above 90% with a standard error in cross validation (SECV) ranging between 12.6 and 15%w/w. Overall, the results of this study indicate that NIR spectroscopy can determine the level or ratio of adulteration in the binary mixtures of minced meat.

Isolation and Molecular Characterization of Clostridium perfringens Toxinotypes F & G in Diarrhoeic Sheep (Ovis aries) Flocks in Southeast of Iran.

Clostridial enteric diseases, called enterotoxemia, are caused by Clostridium perfringens toxinotypes in sheep and other ruminants. This study aimed to describe the molecular characterization of C. perfringens isolates in diarrhoeic sheep (Ovis aries) flocks in the southeast of Iran. Fecal/intestinal samples were collected from diarrhoeic (n=116), dead (n= 13), and healthy (n=63) sheep over four years (2016-2020) and subjected to bacteriological and molecular examinations. The C. perfringens isolates were typed by polymerase chain reaction targeting genes, namely 16SrRNA, CPA, CPB, ETX, IAP, CPE, and NetB. The overall prevalence of C. perfringens was 28.6% among the studied sheep, and there was a significant relationship between its isolation rate and diarrhea (P<0.001). The C. perfringens isolation rate also decreased with animal age (P=0.012) and was significantly higher in late winter and spring (P=0.000). The most prevalent toxinotypes were types A (52.4%), D (22.2%), and F (18.5%), in that order. Moreover, C, G, and B types were found in 4.2%, 1.6%, and 1.1% of the isolates, respectively, and no type E was detected. The CPE gene was detected in 32.3% of all isolates, and the diarrhoeic sheep were most likely to yield CPE+ strains of C. perfringens (93.1%). These findings highlight the importance of CPE+ strains of C. perfringens in sheep enteritis and suggest that the high presence of type F needs to be considered in new clostridial vaccines containing this toxinotype. It is noteworthy that the present study reported the isolation of C. perfringens type F, type G, and the CPE+ strains of type B from diarrhoeic sheep for the first time.

The Discovery of a Bone in the Anterior Fontanelle of the Skull of the Sheep (Ovis aries)

The Discovery of a Bone in the Anterior Fontanelle of the Skull of the Sheep (Ovis aries)

Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze

The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to Polymerase Chain Reaction (PCR) for amplification of Cytochrome b and COI genes using appropriate primers. PCR products were sequenced and analyzed by MEGA X software. The phylogeny analysis categorized Ovis aries including Mundri sheep into three and two groups for Cytochrome b and COI genes respectively. It showed Mundri sheep as a separate group and thus as a separate breed from all other local sheep breeds. Hence the study validates based on Cytochrome b and COI that Mundri sheep is a distinctive breed from the rest of the local sheep breed.

An effective droplet digital PCR method for identifying and quantifying meat adulteration in raw and processed food of beef (Bos taurus) and lamb (Ovis aries)

Meat adulteration caused by economic intentions has long been concerning food safety. Accurate quantification of meat products can distinguish between artificial adulteration and unintentional contamination during its processing or packaging. After determining the species-specific constant and the correlation between mass fraction and copy number of each species, we developed an effective approach-based droplet digital PCR (ddPCR) platform that can identify target species with high detection sensitivity: 13 copies of beef, 6 copies of lamb, 13 copies of pork, 13 copies of chicken, 6 copies of duck, and 6 copies of turkey. Using this method, a level as low as 1% of the adulterated ingredients blended in beef and lamb was accurately quantified. Following the addition of reference species, several quantitative equations were constructed for simultaneous analysis of different species in commercial processed products; even the animal components with a minimum content of 0.5% can be quantified to judge whether the label ingredients are fraudulent. This suggests the feasibility of the proposed strategy for the accurate identification and quantification of animal-derived adulteration according to the processing degree and food commodity.

Molecular Detection of Tick-Borne Bacterial and Protozoan Pathogens in Haemaphysalis longicornis (Acari: Ixodidae) Ticks from Free-Ranging Domestic Sheep in Hebei Province, China.

Ticks and tick-borne pathogens significantly threaten human and animal health worldwide. Haemaphysalis longicornis is one of the dominant tick species in East Asia, including China. In the present study, 646 Ha. longicornis ticks were collected from free-ranging domestic sheep in the southern region of Hebei Province, China. Tick-borne pathogens of zoonotic and veterinary importance (i.e., Rickettsia, Anaplasma, Ehrlichia, Borrelia, Theileria, and Hepatozoon spp.) were detected in the ticks using PCR assays and sequence analysis. The prevalence rates of these pathogens were 5.1% (33/646), 15.9% (103/646), 1.2% (8/646), 17.0% (110/646), 0.15% (1/646), and 0.15% (1/646), respectively. For Rickettsia spp., R. japonica (n = 13), R. raoultii (n = 6), and Candidatus R. jingxinensis (n = 14) were detected for the first time in the province, while several Anaplasma spp. were also detected in the ticks, including A. bovis (n = 52), A. ovis (n = 31), A. phagocytophilum (n = 10), and A. capra (n = 10). A putative novel Ehrlichia spp. was also found with a prevalence of 1.2% in the area. The present study provides important data for effectively controlling ticks and tick-borne diseases in the Hebei Province region of China.

Association and Polymorphism of the APOA5 Gene Related to Carcass Characteristics and Lamb Quality in Sheep

The apolipoprotein A-V (APOA5) gene is a candidate gene that plays an important role in carcass characteristics and lamb quality. This study aimed to association and polymorphism of the APOA5 gene with carcass characteristics and lamb quality in sheep. The breed of sheep were used 100 sheep consisting of Thin Tailed Sheep (TTS) (n=55), Compass Agrinac Sheep (CAS) (n=10), Garut Sheep (GS) (n=18), Garut Composite Sheep (GCS) (n=8), and Barbados cross sheep (BCS) (n=9). Identification of APOA5 gene polymorphism was performed using PCR-RFLP method and association study was performed using GLM analysis. The results showed that the APOA5 (g.26929941 C&gt;T) gene polymorphism was polymorphic in TTS, CAS, GS, and GCS, while BCS was monomorphic. The APOA5 gene has three genotypes (CC, CT, and TT). Association showed that the APOA5 was a significantly (P&lt;0.05) associated with carcass percentage and carcass length. The polymorphism of the APOA5 (g.26929941 C&gt;T) was also significant (P&lt;0.05) associated with lamb quality of the pH dan tenderness. The TT genotype showed greater carcass length and pH than the CC and CT genotypes. The CT genotype showed a greater percentage of carcass and tenderness than the CC and TT genotypes. The APOA5 gene helps improve sheep carcass characteristics and lamb quality.

Augmenting workload drives T-tubule assembly in developing cardiomyocytes.

Contraction of cardiomyocytes is initiated at subcellular elements called dyads, where L-type Ca2+ channels in t-tubules are located within close proximity to ryanodine receptors in the sarcoplasmic reticulum. While evidence from small rodents indicates that dyads are assembled gradually in the developing heart, it is unclear how this process occurs in large mammals. We presently examined dyadic formation in fetal and newborn sheep (Ovis aries), and the regulation of this process by fetal cardiac workload. By employing advanced imaging methods, we demonstrated that t-tubule growth and dyadic assembly proceed gradually during fetal sheep development, from 93days of gestational age until birth (147days). This process parallels progressive increases in fetal systolic blood pressure, and includes step-wise colocalization of L-type Ca2+ channels and the Na+/Ca2+ exchanger with ryanodine receptors. These proteins are upregulated together with the dyadic anchor junctophilin-2 during development, alongside changes in the expression of amphiphysin-2 (BIN1) and its partner proteins myotubularin and dynamin-2. Increasing fetal systolic load by infusing plasma or occluding the post-ductal aorta accelerated t-tubule growth. Conversely, reducing fetal systolic load with infusion of enalaprilat, an angiotensin converting enzyme inhibitor, blunted t-tubule formation. Interestingly, altered t-tubule densities did not relate to changes in dyadic junctions, or marked changes in the expression of dyadic regulatory proteins, indicating that distinct signals are responsible for maturation of the sarcoplasmic reticulum. In conclusion, augmenting blood pressure and workload during normal fetal development critically promotes t-tubule growth, while additional signals contribute to dyadic assembly. KEY POINTS: T-tubule growth and dyadic assembly proceed gradually in cardiomyocytes during fetal sheep development, from 93days of gestational age until the post-natal stage. Increasing fetal systolic load by infusing plasma or occluding the post-ductal aorta accelerated t-tubule growth and hypertrophy. In contrast, reducing fetal systolic load by enalaprilat infusion slowed t-tubule development and decreased cardiomyocyte size. Load-dependent modulation of t-tubule maturation was linked to altered expression patterns of the t-tubule regulatory proteins junctophilin-2 and amphiphysin-2 (BIN1) and its protein partners. Altered t-tubule densities did not influence dyadic formation, indicating that distinct signals are responsible for maturation of the sarcoplasmic reticulum.

Whole genome level analysis of the DEATH protein superfamily in sheep (Ovis aries) and their coordination relationship in regulating lactation

BackgroundSheep milk is a nutritional and health-promoting food source for humans. The DEATH superfamily is a conserved protein family, and some of its members are closely related to lactation. Systematic studies of the members of the DEATH superfamily are important for further understanding its functions in the mammary gland during lactation; however, there studies are currently lacking.ResultsHerein, 74 members of the DEATH superfamily were identified in sheep, and phylogenetic analyses indicated that four subfamilies were strongly correlated in evolution. The Ka/Ks calculations demonstrated that negative selection was the primary pressure acting on DEATH members; however, the immune-related gene IFI203 was undergoing strong positive selection in sheep. Furthermore, in the late pregnancy and lactation period, these DEATH genes exhibited similar expression patterns under different nutritional conditions in the mammary gland, and four subfamilies were positively correlated in expression patterns. Additionally, half or more DEATH genes were upregulated in the lactation period, which implied their crucial roles in the lactation of sheep.ConclusionsThe current research contributes to a better understanding of the evolutionary characteristics of the DEATH superfamily and their roles in sheep lactation, and it also provides potential target genes for the molecular breeding of dairy sheep.Graphical

Morphometric analysis of the mandible of ram and ewe romanov sheep (Ovis aries) with 3D modelling: A CT study.

2D images view hardly measurement points due to the overlap of anatomical features. This challenge is overcome by 3D modelling. In particular, images obtained by computed tomography are converted into 3D models through certain software. In sheep breeds with high polymorphism, some changes have occurred in their morphology due to both environmental and genetic factors. In this context, determining the osteometric measurements of sheep and revealing breed-specific characteristics provide very important data for forensic, zooarchaeological, and developmental sciences. Mandibular reconstruction measurements are used to reveal differences between species and between sexes and for treatment and surgery in many fields of medicine. In the present study, morphometric characteristics were determined by 3D modelling from computed tomography images obtained from mandibles of Romanov ram and ewe. For this purpose, mandibles of 16 Romanov sheep (eight females and eight males) were used. They were scanned using a 64-detector MDCT device at 80 kV, 200 MA, 639 mGY, and 0.625 mm slice thickness. CT scans were recorded in DICOM format. Reconstructions of the images were made using a special software program. Volume and surface area measurements were made with 22 osteometric parameters of the mandible. GOC-ID had a statistically significant positive correlation with GOC-ID, PC-ID, GOC-MTR, GOC-PTW, GOC-FMN, PMU, MDU, PDU, DU, GOV-PC, GOV-IMD, MTR-MH, MO-MH, FMN- ID, BM, MG, and CG (p < 0.01). GOC-ID had a statistically significant correlation with MTR-ID, GOV-CR, PTW-MH and SI (p < 0.05). When the CR-PC measurement point was examined, it was observed that it had no statistically significant correlation with all measurement points (p > 0.05). As a result of the measurement, it was found that the volume and surface areas were higher in rams than in ewes. The morphometric data obtained would be a reference income in the fields of zoo-archaeology, anatomy, forensics, anaesthesia, surgery, and treatment.