Two sulfuric acid speleogenesis (SAS) cave sites in northern Wyoming host speleogenetic byproducts that have been dated using the uranium-series method and thus provide the timing of the SAS events. Gypsum crusts that formed as byproducts of SAS in Lower Kane Cave, exposed by Bighorn River incision into the Little Sheep Mountain anticline, yield uranium-series dates of 20.5 ± 0.2 ka to 16.2 ± 0.2 ka that represent the timing of late stage speleogenesis of that cave. A calcite mammillary engulfed by elemental sulfur that formed during SAS of Shoshone Canyon Conduit Cave, exposed by Shoshone River incision of the homonymous canyon, yielded a uranium-series age of 174 ± 5 ka and represents late stage speleogenesis of that cave, although the process continued after the cave was elevated above the water table until at least 100 ± 5 ka. In addition to the timing of speleogenesis, the U-series results provide incision rates for these two canyons. The rate of canyon incision for the last 200 kyr by the Bighorn River in Little Sheep Mountain Canyon is measured as 190 ± 50 m/Ma, and for the Shoshone River in Shoshone Canyon as 400 ± 40 m/Ma. Using the Bighorn River incision rate, Upper Kane Cave located 33 m above Lower Kane Cave has a projected age of 170 ± 60 ka. We suggest that climatically driven hydrologic mechanisms rather than magmatic ones likely produced the pulses of SAS at these two cave sites.