Sheep Dung Research Articles

Effect of larval density and substrate quality on the wing geometry of Stomoxys calcitrans L. (Diptera: Muscidae)

BackgroundIn insects, oviposition decisions may lead to egg deposition in substrates with different larval density and nutritional levels. Individuals developing in such substrates may present plasticity in their phenotype. Here, we investigated the effect of two factors related to oviposition decisions, namely larval density and substrate quality, on the wing size and wing shape of the stable fly, Stomoxys calcitrans L. (Diptera: Muscidae).MethodsWe reared S. calcitrans larvae at different densities (5, 15 and 25) and on different substrates (camel, cow, donkey and sheep dung). For each fly that emerged, we recorded body weight, and detached, slide-mounted and photographed the right wing. Next, we collected 15 landmarks on each photographed wing, and applied geometric morphometric analysis to assess variation in wing size and wing shape of S. calcitrans across the different larval densities and substrate types.ResultsWe observed that wing size and wing shape of S. calcitrans were affected by larval density and the nature of the developmental substrate. Flies reared in a group of 5 had larger wing centroid size, wing length, wing width, wing area and wing loading compared with those reared in a group of 25. Also, flies developed in donkey and sheep dung had larger wing centroid size, wing length, wing width, wing area and wing loading in comparison with those grown in camel and cow dung. Canonical variate analysis followed by discriminant analysis revealed significant wing shape variation in S. calcitrans across the different densities and substrates. Wing size had a significant but weak positive effect on wing shape.ConclusionsThis study demonstrates the high sensitivity of S. calcitrans wings to variation in larval density and developmental substrate, and that use of landmark-based geometric morphometric analysis could improve our understanding of how flies of veterinary importance respond to environmental variability.

Larval experience of stable fly, Stomoxys calcitrans Linneaus, 1758 (Diptera: Muscidae) does not influence oviposition preference in gravid females

1. Hopkins' host selection principle (HHSP) proposes that, in holometabolous insects, gravid females prefer to oviposit on their developmental substrate. This hypothesis is widely explored in phytophagous insects, but few studies have considered blood‐feeding insects. In this study, the HHSP was tested using the haematophagous stable fly, Stomoxys calcitrans (Diptera: Muscidae). Using no‐choice and two‐choice tests, the study first sought to demonstrate whether S. calcitrans larvae recognise their rearing substrate.2. It was found that when a rearing substrate is offered to S. calcitrans larvae singly (no‐choice) or associated with a non‐rearing substrate (two‐choice), they were able to recognise the substrate in which they developed. This ability disappeared when larvae were transferred to another substrate for 5 h.3. Next, using oviposition bioassays, it was investigated whether information gathered by pre‐imaginal stages during their development can persist into the adult stages after metamorphosis. It was hypothesised that gravid females emerging from clean and uncleaned pupae reared on sheep and camel dung would still prefer to lay on their rearing substrate. The results revealed that gravid females did not prefer to oviposit on their developmental substrate. In addition, failure to recognise and prefer their developmental substrate was found in gravid females that had emerged from puparia that were cleaned as well as those contaminated with sheep dung.4. It is concluded that S. calcitrans adult preference is not influenced by their offspring's experience. Overall, the HHSP was not supported by our study, suggesting that this principle may not apply to generalist insects characterised by high plasticity in oviposition substrate acceptance.

Egg-laying decisions based on olfactory cues enhance offspring fitness in Stomoxys calcitrans L. (Diptera: Muscidae)

Selection of oviposition substrate is critical in holometabolous insects. Female stable flies, Stomoxys calcitrans, locate and select vertebrate herbivore dung in which they lay their eggs. However, the preference for vertebrate herbivore dung by S. calcitrans females, its fitness consequences for offspring, and the semiochemicals used to locate and select oviposition substrates remain unclear. Using oviposition choice tests and life table bioassays we found that gravid female S. calcitrans prefer to oviposit on donkey and sheep dung, which also improves the performance of their offspring. GC-MS analysis followed by random forest classification identified β-citronellene and carvone as the most important predictive volatile organic compounds of donkey and sheep dung, respectively. In multiple choice oviposition bioassays, S. calcitrans laid more eggs in wet sand containing β-citronellene and carvone than in other treatments. The attractiveness of these compounds was confirmed in a field trial, with traps baited with β-citronellene and carvone catching more S. calcitrans. We conclude that gravid female S. calcitrans use semiochemical cues to choose oviposition substrates that maximise offspring fitness.

Potentiality of Indigenous Earthworms (Perionyx excavatus, Perrier and Lampito mauritii, Kinberg) in Managing Lignocellulosic Wastes for Organic Farming

The lignocellulosic wastes, cashew leaf litter (CLL) admixed with various animal dungscowdung (CD), sheepdung (SD) and horsedung (HD) have been subjected to vermicomposting by using indigenous earthworms, Perionyx excavatus and Lampito mauritii and to produce value added vermicompost. Four different combinations of each [100% dung alone, 3 : 1 (75% dung + 25% CLL), 2 : 2 (50% dung + 50% CLL) and 1 : 3 (25% dung + 75% CLL)] vermibeds were prepared for vermicomposting process. After 60 days, the vermicompost and worm-unworked normal compost were harvested and characterized and growth (biomass), reproductive performance of earthworms-cocoon production, hatchling number and recovery of vermicompost were also studied. The obtained results clearly showed that vermicompost from CLL admixed with CD at 2 : 2 ratio had lower pH, organic carbon, C–N ratio, C–P ratio, lignin, cellulose, hemicellulose and phenol content, and higher nitrogen, phosphorus, potassium, dehydrogenase and humic acid content than the raw substrates and normal compost. Also pronounced and better earthworm activity was found in the above combination for both species. Hence organic farming practices using vermicompost promise an alternative to the existing practices and proved as a sustainable approach.

Response of vegetation to sheep dung addition in a degraded Cerrado area

ABSTRACT Cerrado is the second largest biome in Brazil and is classified as a biodiversity hotspot. The establishment of hydroelectric power stations in Brazil originated degraded areas due to the removal of soil for construction of dams, in which native vegetation sometimes fails to reestablish due to the bad soil conditions. Sheep excrete most of the nutrients they ingest, such as phosphorus, calcium, potassium and nitrogen. This study aimed at investigating whether sheep dung contributes to the improvement of soil quality and stimulates the process of ecological succession in areas affected by the construction of the hydroelectric power station of Ilha Solteira. Four areas were selected, located at the Experimental Farm of Unesp Ilha Solteira/SP, and ten plots of 1 m2 each were established. From October 2014 to December 2015, five replicates received 150 g fresh sheep dung every 15 days. In December 2015 soil sample from top 0.10 m was collected for the determination of soil attributes. Aboveground biomass was also collected, separated into four functional groups, oven-dried and weighed. Data were analysed to check the effect of dung addition upon soil and vegetation variables. Dung addition stimulated the development of several functional groups and changed soil nutrient concentrations in all four studied areas.

Effects of grazing season and stocking rate on seed bank in sheep dung on the semiarid Loess Plateau

Endozoochorous dispersal of seeds by livestock has long attracted the attention of grassland scientists, but little is known about seed dispersal via Tan sheep dung on the dry grasslands of the Loess Plateau. We investigated the composition of dung seed bank of Tan sheep under summer and winter rotational grazing regimes at different stocking rates (2.7, 5.3 and 8.7sheepha–1), and assessed the relationships between seed mass and shape, egested seedling density and species richness and diversity. We also assessed the effects of stocking rate and grazing season on seedling density and species richness and diversity of the dung seed bank. Seeds of 10 pasture species germinated from Tan sheep dung with only two being annual species (Eragrostis pilosa and Chenopodium glaucum). The mean seed mass (±s.e.) was 2.59±0.23mg, ranging from 0.10mg (Cleistogenes songorica) to 10.59mg (Thermopsis lanceolata), and the mean seed shape index (±s.e.) was 0.10±0.02, ranging from 0.03 (Lespedeza bicolor) to 0.19 (Stipa bungeana). Species richness and diversity and seedling density decreased with increasing seed mass and shape index, suggesting that small, round seeds are most suitable for endozoochorous dispersal. Stocking rate had no effect on the number of germinated seeds that collected from summer or winter grazing pastures. Species richness and diversity and seedling density were greater in winter grazing pastures than in summer grazing pastures. Jaccard coefficients of similarity between the Tan sheep dung bank and aboveground vegetation were <0.5 for all stocking rates, indicating their weak relationship, but were significantly higher for winter than summer grazing. Plant seeds on the Loess Plateau could disperse through Tan sheep grazing, endozoochory can increase the heterogeneity of rotationally grazed plant communities, and this dispersal mode is an adaptation of plants to the harsh environment of the semiarid areas.

Intraspecific variation in litter palatability to macroarthropods in response to grazing and soil fertility

Abstract Clarifying the functional consequences of intraspecific trait variability in response to interacting trophic levels would provide a significant improvement in our understanding of above‐ground–below‐ground linkages. In particular, the effects of grazing on plant traits may translate into altered litter quality, with potentially important consequences for litter‐feeding decomposers. Plant and litter variability in response to grazing is expected to depend on soil fertility levels, with tolerance and defensive strategies more commonly expressed on fertile and poorer soils, respectively. However, how grazing and fertility interactively alter litter quality and palatability to detritivores has not been explored yet. We conducted a cafeteria experiment with three common millipede (Diplopoda) species feeding on leaf litter from two plant species, the grass Bromopsis erecta and the forb Potentilla verna. Each millipede was offered a binary choice between litter types produced by the same plant species, but sampled in plots with distinct herbivory and fertilization status: litter originating from grazed areas or from 1‐year sheep exclosures, both in native areas and in adjacent paddocks that received chemical N and P fertilization, as well as litter from a 25‐year sheep exclosures in the native area. We found that fertilization and herbivore exclusion interactively affected Bromopsis litter quality and palatability, whereas Potentilla was much less affected. Bromopsis litter palatability was not affected by grazing when litter was collected in native plots, except for the long‐term exclosure which led to low palatability. In contrast, and in line with our expectations, herbivory was associated with much higher palatability in fertilized plots. The changes in palatability were associated with important alterations of litter quality. Overall, our study demonstrates that intraspecific variation in litter can have profound consequences for soil functioning. It emphasizes the role of grazing as a key, but plant species‐specific factor controlling litter intraspecific variability, and its complex interaction with soil fertility level. Moreover, our results advocate for a better understanding of the response of the different organisms involved in the decomposition process, in particular litter‐feeding macro‐detritivores. We encourage future studies aiming at disentangling the various and complex relationships between above‐ground processes such as herbivory and soil functioning.

Microbial pathways for nitrous oxide emissions from sheep urine and dung in a typical steppe grassland

The aim of this study was to determine the responses of nitrifiers and denitrifiers to understand microbial pathways of nitrous oxide (N2O) emissions in grassland soils that received inputs of sheep excreta. Sheep dung and synthetic sheep urine were applied at three different rates, simulating a single, double, or triple overlapping of urine or dung depositions in the field. Quantitative PCR and high-throughput sequencing were combined with process-based modeling to understand effects of sheep excreta on microbial populations and on pathways for N2O production. Results showed that emissions of N2O from urine were significantly higher than from dung, ranging from 0.12 to 0.78 kg N2O-N ha−1 during the 3 months. The N2O emissions were significantly related to the bacterial amoA (r = 0.373, P < 0.001) and nirK (r = 0.614, P < 0.001) gene abundances. It was autotrophic nitrification that dominated N2O production in the low urine-N rate soils, whereas it was denitrification (including nitrifier denitrification and heterotrophic denitrification) that dominated N2O production in the high urine-N rate soils. Nitrifier denitrification was responsible for most of the N2O emissions in the dung-treated soils. This study suggests that nitrifier denitrification is indeed an important pathway for N2O emissions in these low fertility and dry grazed grassland ecosystems.

Potentiality of indigenous earthworms (Perionyx excavatus, perrier and lampito mauritii, kinberg) in managing lignocellulosic wastes for organic farming

The lignocellulosic wastes, cashew leaf litter (CLL) admixed with various animal dungscowdung (CD), sheepdung (SD) and horsedung (HD) have been subjected to vermicomposting by using indigenous earthworms, Perionyx excavatus and Lampito mauritii and to produce value added vermicompost. Four different combinations of each [100% dung alone, 3 : 1 (75% dung + 25% CLL), 2 : 2 (50% dung + 50% CLL) and 1 : 3 (25% dung + 75% CLL)] vermibeds were prepared for vermicomposting process. After 60 days, the vermicompost and worm-unworked normal compost were harvested and characterized and growth (biomass), reproductive performance of earthworms-cocoon production, hatchling number and recovery of vermicompost were also studied. The obtained results clearly showed that vermicompost from CLL admixed with CD at 2 : 2 ratio had lower pH, organic carbon, C–N ratio, C–P ratio, lignin, cellulose, hemicellulose and phenol content, and higher nitrogen, phosphorus, potassium, dehydrogenase and humic acid content than the raw substrates and normal compost. Also pronounced and better earthworm activity was found in the above combination for both species. Hence organic farming practices using vermicompost promise an alternative to the existing practices and proved as a sustainable approach.

Comparative Study on the Efficacy of Biogas Production from Mixture of Animal Waste and Pistia stratioles (Water Lettuce)

Biogas was produced from the mixture of Sheep dung and Water lettuce (Pistia stratioles) in the ratio of 2:1 and 1:2 over a period of 77 days. Proximate analysis was done to determine the physicochemical parameters of each dried sample. Daily temperature of the laboratory, initial and final pH of the digesters containing slurry was recorded. This work was done to investigate the production of biogas from the mixture of sheep dung and water lettuce in other to improve the biogas production. Proximate analysis was done according to the methods described by Khanadainal and Malidi (1981) and AOAC (1990). Sheep dung and water lettuce was mixed in the ratio of 2:1 and 1:2 each in 900g empty milk tin and mixed with water to form slurry. Initial pH of each slurry was determined by inserting a digital pH meter. Final pH was determined after digestion. The gas was collected through a pipe connected to the digester which was inserted in to a measuring cylinder filled with water and inverted in a container with water. Physical parameters determined for both sheep dung and water lettuce respectively includes: Moisture (11% and 8.5%), Volatile Solid (6.2% and 8.7%) and Ash (19.5% and 44.5%). Initial and final pH of each digester was (7.30 and 7.20), and (6.67 and 6.38) for 2:1 and 1:2 ratio respectively. A total biogas of 17983.33cm3 and 8546.67cm3 were recorded by 2:1 and 1:2 ratio of sheep dung and water lettuce respectively. The production temperature ranges from 28oC to 36oC. Nature of substrates affect biogas production as revealed in this research.&#x0D;

Methane production through anaerobic co-digestion of sheep dung and waste paper

A large amount of sheep dung (SD) produced on farms in China is a potential feedstock for production of clean energy in the form of biogas. Similarly, waste paper can be a notable energy source that is worth exploiting. In this study, we assessed the feasibility of co-digesting nitrogen-rich SD with the carbon-rich corrugated board (CB) or waste office paper (OP) in varying volatile solids (VS) ratios, to produce methane. Synergistic effect of co-digesting SD with CB and SD with OP on methane production was found in this study. The highest methane yields of 151.62 and 198.85mL/g-VS were obtained during the co-digestions of SD with CB at 4:1 ratio (SDCB) and SD with OP at 2:3 ratio (SDOP), respectively. High-throughput 16S rRNA gene sequencing demonstrated that the microbial diversity and richness in SDCB and SDOP co-digests were higher than in SD and OP mono-digests, respectively. Characteristic bacteria and archaea in the digests were strongly substrate-related and might contribute to methane production. The validated results indicated that methane production through anaerobic co-digestion of SD and waste paper can be an efficient way that could not only reduce environmental pollution but also contribute to methane production.

Going native, going local: revegetating eroded soils on the Falkland Islands using native seeds and farmland waste

AbstractRemote island ecosystems are vulnerable to human disturbance and habitat destruction, yet they often have limited capacity to revegetate degraded habitats, especially with native species. To revegetate degraded island habitats, practitioners often rely on importing non‐native species, thereby increasing the number of introduced species on islands. In this study, we investigated the effectiveness of sowing wild collected native seeds and locally sourced treatments for revegetating different eroded soil types (clay, peat, and sand) across the Falkland Islands. A seed mixture of 15 native species was sown with different supportive treatments (sheep dung, sheep dags [woolly off‐cuts], and geotextile matting [coir]) and their combinations. After 1 year, native seeds provided up to 70% plant cover and accrued 1.98 kg/m2 in biomass. Three key native species Elymus magellanicus, Poa flabellata, and Poa alopecurus occurred in 64, 50, and 50% of all sown plots. However, supportive treatments equally facilitated the colonization and establishment of non‐native species. At the same time, there was no difference in native plant cover and biomass across different treatments or soil types, although in the absence of supportive treatments there was little to no revegetation. Thus, locally sourced treatments (i.e. sheep dung and dags) may provide an equally effective but low‐cost alternative to imported treatments (i.e. geotextiles). We further discuss challenges of integrating revegetation using native seeds and livestock grazing on the Falkland Islands. Our study demonstrates that native species and local treatments can provide a rapid approach to revegetating degraded island habitats.

Low Input Improvement of Degraded Grassland in Semiarid Area of China : 1. Evaluation of sheep dung as a water retention agent

Low Input Improvement of Degraded Grassland in Semiarid Area of China : 1. Evaluation of sheep dung as a water retention agent

Increasing crop diversity increased soil microbial activity, nitrogen-sourcing and crop nitrogen, but not soil microbial diversity

The relationships between crop diversity, soil microbial diversity and agroecosystem functioning are not well understood. Soils under wheat monoculture, wheat–medic, and wheat–medic plus clover rotations from a 19-year-old wheat rotation trial in South Africa were measured for soil microbial functional and genetic diversity using community-level physiological profiling and automated rRNA intergenic spacer analysis. A 15N–13C dual isotope mixing model determined the nitrogen (N) sourcing when wheat was supplied with N from fertiliser and sheep dung (monoculture), or fertiliser, diazotrophy from one or two legumes, and sheep dung (wheat in rotation). Historical wheat yields and foliar [N] were 28% and 106% higher in wheat–legume rotations compared with wheat monoculture. Increasing crop diversity was related to increased soil microbial activity, but not increased microbial richness or diversity, which depended more on known abiotic drivers of microbial community structure. The δ15N mixing models revealed an increasing dependence on legume-derived N with increasing legume species in rotation. This suggests that enhanced N cycling and yield in crop–legume rotations is not a result of microbial diversity per se, but rather increased microbial activity when keystone legume species and their associated N2-fixing symbionts were present.

Domestic and ritual use of plants and fuels in the neolithic cave of Alepotrypa, southern Peloponnese, Greece: The wood charcoal and phytolith evidence

The study presents the combined results of wood charcoal and phytolith analysis at Alepotrypa Cave, southern Peloponnese, Greece. The cave preserves rich cultural remains (hearth and floor constructions, pits and platforms, human bone scatters, massive quantities of fine pottery, lithic artefacts and ornaments) spanning the late Early to the Final Neolithic. The studied macro and micro-remains come from two distinct areas of the cave, the anterior chamber (close to the entrance of the cave) and the interior chambers (including a small fresh water lake), which, as has been suggested by several lines of evidence (analyses of cultural remains, human bones and micromorphology), were used for domestic and ritualistic purposes respectively. The aim of this study is two-fold: a) to investigate the local vegetation, and woodland management, b) to understand the use of plants and use of space along the habitation history of the cave exploring the possibility of a domestic setting for the anterior chamber and a ritualistic one for the interior. Wood charcoal and phytolith analyses support the two modes of usage; different fuel types in the hearths of the interior and anterior chambers of the cave along with different activities are documented. The anterior preserves well prepared clay floors and platforms with some cereal remains indicating light processing or consumption. The hearths in this area were fed with leafy branches from the open vegetation of the surrounding rocky slopes that included various scrub plants and scattered drought-resistant trees. Τhrough time and probably as a response to increased demand due to more frequent and longer-lasting use of the cave, neolithic people expanded their fuel-procurement activities to nearby evergreen woodland and deciduous oak thickets. By contrast the interior preserves evidence of ritualistic activities supported by the use of selected types of fuel, i.e. composted sheep dung along with firewood from scrub vegetation and small diameter wood of Fabaceae, Cistus sp. and Phillyrea/Rhamnus alaternus. The excellent burning qualities of composted dung and the ease of transportation of such material as well as of the small size firewood would explain their preferential use in the interior chambers where access was exceptionally difficult. Nevertheless, the slow-burning glow and smell of dung under the light of Pinus nigra resinous wood torches may have enhanced the powerfully evocative atmosphere of the interior chambers serving ritual purposes.

Potential of biogas production to reduce firewood consumption in remote high-elevation Himalayan communities in Nepal

Remote communities in the Nepalese mountains above 2500 m a.s.l. belong to the most precarious in the world. Inhabitants struggle for the minimum in terms of safe drinking water, food and sanitation. Reliable, affordable and clean energy for cooking, room heating and warm water for personal hygiene is often lacking and dependency on firewood very high. The remoteness and unlikeliness of electric grid connection in the coming decades make a diversified energy supply from renewable local resources crucial. Small-scale anaerobic digestion (AD) of organic substrates has been used for long in rural areas of developing countries to produce biogas as energy source and recover residue as organic fertilizer. AD is challenging at high elevations due to year around lower ambient temperatures and lower annual biomass production per area compared to lowlands. Nevertheless, examples of operational household AD exist even above 3000 m a.s.l. in the Andes. Here we compare firewood consumption with biogas potential from organic substrates in a community with 39 households at 3150 m a.s.l. in Jumla District, Nepal. In five households with varying numbers of members and animals kept, mean firewood use and its energy content per capita (cap) and day (d) were 2.1 kg or ca. 25 MJ in spring and 2.3 kg or ca. 28 MJ in winter. Easily available substrates include cow, sheep and horse dung from overnight shelters and human excrements from pit latrines, amounting on average to 1.7 kg wet weight (kgww ) cap−1 d−1 in spring and 2.2 kgww cap−1 d−1 in winter. Adjusted to normal conditions (Nm3 at 0 °C, 1013.15 hPa), these substrates yielded on average 0.08 Nm3 cap−1 d−1 biogas in spring and 0.12 Nm3 cap−1 d−1 in winter (35–60% methane content) in biochemical methane potential (BMPs) tests at 36 °C. This could provide up to 60% of basic cooking needs on average and up to 75% in a “typical” household in terms of members and animals kept. Of the overall thermal energy needs including also room heating ca. 10–20% could be covered, substituting 0.1–0.4 (mean: 0.2) kg firewood cap−1 d−1 . If only animal dung and human excrements are considered, no competition for resources arises as residues can still be used as organic fertilizer. This study supports the design and introduction of planned pilot digesters integrated into on-going community development including pit latrines for substrate availability, greenhouses as possible way of thermal insulation, and planned pico-hydropower plants to use excess electricity during the night for digester heating.

Animal dung availability and their fertilizer values in a context of low soil fertility conditions for forage seed and crops production in Benin (West Africa)

Livestock manure, feed biomass fed to animals that pass through digestive tract undigested and urine excreted from subsequent tissue metabolism, is conventionally termed as wastes. To optimize the use of animal manure for the purpose of agronomic processing or valorization, it is essential to know its availability and plant nutrients composition. The use of reference values ​​is a quick method of estimation. However, books on farm fertilizers generally offer only an average value that is not representative of the diversity of situations. The aim of this study was to (1) estimate the quantity of manures from cattle, sheep, goats, pigs and poultry, (2) determine the physico-chemical characteristics and plant nutrient contents of these droppings and (3) identify the inter-relationships between the physical characteristics (pH, EC and dry matter(DM)) and the most essential macronutrients (N, P and K). A total of 30 animal groups (herds or flocks) were survived per species and a total of 30 samples were collected over twelve months (January 1st to December 31, 2016) for DM, pH, electrical conductibility, nitrogen, phosphorous, potassium, calcium, magnesium and sodium contents for each sample. According to animal population of the country, the results of the study showed that, an amount of 1.630600 tons DM of cattle manure, 227800 tons DM of sheep dung, 136,900 tons DM of goat dung, 122,400 tons DM of pig dropping and 36,500 tons of poultry excreta are annually available in Benin. Physico-chemical and analyzes of droppings showed significant differences (P˂0.01) between mineral compositions of these dropping. Poultry manure are richer in macronutrients than other types of animal manure (N = 11.7 ± 3.9, P = 4.6 ± 2.3, K = 7.6 ± 1.3, Ca = 41.2 ± 16.7 g/kg), followed by goat and sheep manure (N = 6.0 ± 3.7, P = 4.9 ± 3.9, K = 7.3 ± 3.3, Ca = 7.7 ± 3.8 g / kg and N = 6.7 ± 2.3, P = 4.4 ± 1.5, K = 7.3 ± 3.6, Ca = 7.7 ± 2.6 g / kg, respectively). Mean macronutrient compositions of swine dropping were: (N = 4.5 ± 2.0, P = 1.4 ± 0.8, K = 2.9 ± 0.8, Ca = 1.8 ± 0.9 g / kg). The animal manure that showed the lowest levels of these three macronutrients were those of cattle (N = 3.0 ± 0.6, P = 0.6 ± 0.1, K = 4.1 ± y, Ca = 6.4 ± 3.1 g/kg). Correlations between physico-chemical properties (pH, EC, DM) and nutrient concentration showed that dry matter (DM) and electrical conductivity (EC) could be used to estimate nutrient (nitrogen, phosphorus and potassium) concentrations. The results vary widely depending on the source and type of dejection but they are a good basis for choosing rational and optimal soil fertilization for crop and forage productions.

Do grazing intensity and herbivore type affect soil health? Insights from a semi‐arid productivity gradient

Summary Grazing is one of the most widespread forms of intensive management on Earth and is linked to reductions in soil health. However, little is known about the relative influence of herbivore type, herbivore intensity and site productivity on soil health. This lack of knowledge reduces our capacity to manage landscapes where grazing is a major land use. We used structural equation modelling to assess the effects of recent (cattle, sheep, goats, kangaroos and rabbit dung) and historic (cattle, sheep/goat livestock tracks) herbivore activity on soil health at 451 sites across 0·5 M km2 of eastern Australia. We assessed the direct and indirect effects of increasing herbivore intensity, using dung and livestock tracks, on 15 morphological, physical and chemical attributes that are indicative of soil health, and we used these attributes to derive three indices representing the capacity of the soil to maintain its structural integrity (stability), cycle nutrients (nutrients) and maintain water flow (infiltration). Grazing had negative effects on the three soil health indices, but these effects varied with productivity. Grazing intensity was associated with strong reductions in the stability and nutrient indices under low productivity, but these effects diminished with increasing productivity. Herbivore effects on individual attributes varied in relation to productivity level and were strongly herbivore specific, with most due to cattle grazing, and to a lesser extent, sheep, goats and rabbits. Few effects due to kangaroos or historic grazing by livestock were observed. Synthesis and applications. Our study shows that livestock and rabbits degrade soil health through grazing, and its effects are strongest under low or moderate productivity; however, kangaroo effects are benign. Our findings support calls for resource management agencies to consider site productivity, as well as herbivore type and intensity, when developing strategies to manage grazing by livestock, and feral and native herbivores.

Nitrogen loss factors of nitrogen trace gas emissions and leaching from excreta patches in grassland ecosystems: A summary of available data

Patches of excreta voided by grazing animals are nitrogen (N) transformation hotspots in grassland ecosystems and an important source of N trace gas emissions and leaching. Previous studies have focused on individual N losses from excreta, but no quantitative analysis has been performed on all the N losses via N trace gas emissions and leaching. To better understand the fate of N in excreta patches, we summarized 418, 15, 65, 22, 54, 11, and 81 measurements of nitrous oxide (N2O), nitric oxide (NO), ammonia (NH3), and ammonium (NH4+) leaching, nitrate (NO3−) leaching, dissolved organic nitrogen (DON) leaching, and aboveground plant N uptake, respectively. The results based on field studies indicated that the average fractions of N lost via N2O were 0.28%, 0.76%, 0.08%, and 0.35% for cattle dung, cattle urine, sheep dung, and sheep urine, respectively. Only 0.01–0.12% of excreta N was lost via NO, whereas 1.69–12.7%, 0–4.58%, 16.4–24.6%, and 1.43–5.91% were lost by NH3 and NH4+, NO3−, and DON leaching, respectively. Aboveground plant parts assimilated 10.4–31.4% of the excreta N. The N lost via N2O from urine patches decreased as NH3 losses increased, and greater NO3− leaching occurred with lower plant N uptake. The combined N2O emission factors for dung and urine from cattle and sheep were 0.59% and 0.26%, respectively. Each N loss factor was much higher in urine patches than in dung patches, irrespective of animal type. This study provides general estimates of N losses and plant N uptake from excreta patches on grazed grassland based on currently available field data. More field studies are needed in the future with longer measurement periods from a wide range of climate zones to refine these N loss factors.

Bone alterations in fumiers: Experimental approach

This paper focuses on the study of bone alterations in fumier deposits from an experimental point of view. Fumiers are characteristic of livestock enclosures in the Mediterranean area used from the Neolithic to the Bronze Age. They originate from the process of burning livestock dung piles to reduce the volume of dung accumulation and to rid these spaces of parasites. Therefore, diverse taphonomic processes take part in the formation of these types of deposits, making it difficult to interpret them, despite numerous multidisciplinary studies. This work specifically focuses on bone burning alterations.Two experiments were set up consisting of bone burning in dung piles. Sheep and goat dung was used with different matrix, texture and humidity features. The sample was made up of defleshed fresh, boiled and dry bone fragments from small and medium-sized species.As seen in previous experiments, the burning time and temperature in the two experiments varied due to these different dung features. The resulting bone sample was analyzed using taphonomic methods. Bone alterations essentially depend on the time and intensity of the dung burning. Bone condition is a secondary determining factor. The color degrees were high and coherent with the degree of dung alteration. Cortical damage was more abundant in fresh and boiled bones than in dry bones. No meaningful differences were identified with regard to bones burned in hearth combustions.