Shear Stress Conditions Research Articles

Improved system for measuring rheological properties of thickened liquid using an inclined parallel plate and optical sensor.

The purpose of this study was to improve the method of using an inclined parallel plate (IPP) in directly estimating the yield stress and evaluating the properties of a thickened liquid. The flow curve of the relationship between the shear rate and shear stress of a liquid thickened with a xanthan gum agent was predicted using the Herschel-Bulkley fluid model ( ). We supposed that the yield stress and the result of a line spread test (LST) indicate the deformation state and the flow state of shear stress ( ), respectively. , the yield stress estimated adopting a rotational viscometer and LST, was investigated for three liquids thickened with xanthan gum at four concentrations (C) at intervals of 0.5 wt% within the range 0.5-2.0 wt%. Linear plots of the relations of versus and and the LST show that with an increase in , the resistance force ( and ) increases until flow starts, after which viscosity increases. We suggest that yield stress estimated with the IPP method effectively indicates the rheological properties of thickened liquids.

Undrained cyclic behaviour of loess with initial shear stress: A focus on failure mode

Soils in the field conditions, such as in cut slopes or under embankments, often sustains an initial static shear stress prior to the cyclic loadings. Current knowledge on the undrained cyclic behaviour with the presence of initial shear stress has been mainly built on sands. Experimental studies for loess that contains adequate information in this respect is lacking. To fill this gap, this paper presents results from a series of undrained cyclic triaxial tests on the reconsititued and intact loess samples, subjected to different initial static shear stresses. It was found that the existence of initial static shear stress has a significant impact on the failure modes of loess. Three failure modes were observed in the loess samples, including the flow failure, the cyclic mobility and the plastic strain accumulation, among which the flow failure in the reconsitituted loess sample was more destructive because of its sudden nature. On the basis of the experiments, we propose a chart to facilitate the identification of the failure modes of loess by taking into account the influence of initial stress state and the excess pore water pressure (Δu). Specifically, the changes of Δu in the first two cycles are used to distinguish the flow failure and other failure modes. The proposed metod is further validated using the literature data, showing a satifactory predictive peformance. The outcome of this study provides a guideline that is useful not only for evaluating undrained cyclic behaviour of loess, but also for predicting the failure modes without the need to load samples to failure.

Understanding fabric evolution and multiple liquefaction resistance of sands in the presence of initial static shear stress

Recent field observations have shown that mainshock and subsequent aftershock events can induce multiple-liquefaction events, in which soil liquefies several times at the same site. However, the fundamental mechanism of multiple liquefaction in most previous studies is under level ground conditions, and the cyclic loading condition is symmetric under such conditions. In this study, we conduct 3D DEM simulations of multiple-liquefaction processes considering initial static shear stress. 14-sphere clumped models are used to reconstruct the realistic grain shape of Toyoura sand particles. Cyclic liquefaction and reconsolidation tests are carried out alternately five times. The soil samples with various initial static shear stresses are sheared up to different maximum shear strains in each liquefaction stage. We investigate the liquefaction behavior and volumetric compression during multiple-liquefaction stages considering initial static shear stress. In addition, the evolution of contact-based fabrics, including coordination number (Z) and degree of fabric anisotropy (ac), are analyzed during the whole liquefaction and reconsolidation tests. Furthermore, we establish the relations among contact-based fabrics (Z and ac), volumetric compression and liquefaction resistance. The results reveal that in stress reversal cases, the maximum shear strain is the governing factor that influences the multiple-liquefaction resistance. Conversely, the static shear stress becomes the dominant factor affecting the multiple-liquefaction resistance in non-stress reversal cases. Moreover, changes in multiple-liquefaction resistance are attributed to the microstructure of soils represented by Z after reconsolidation.

Nonlinear deflection characteristics of damaged composite structure theoretical prediction and experimental verification

This research derived a nonlinear computational model for the prediction of deflection responses of the damaged layered composite structures. The model validity was verified using in-house experimentation. The composite panel model has been derived mathematically using the higher-order polynomial kinematics without non-zero shear stress conditions and Green’s strain for the geometrical nonlinearity. The nonlinear deflection responses of combined damage curved shell structures are evaluated numerically utilizing the nonlinear isoparametric finite element technique in conjunction with the direct iterative method. By setting the necessary convergence criteria, the model accuracy has been explored by comparing the deflections of the published literature (the maximum deviation observed is 6.97%). Additionally, the experimentation is done on damaged laminated shell structures, and results are compared to show the proposed model’s efficacy (maximum deviation observed is 2.17%). Finally, a number of numerical examples are solved to assess the effects of the material, geometry and damage-related parameters on the nonlinear deflections of the shell structure.

Cyclic failure characteristics of silty sands with the presence of initial shear stress

Liquefaction of silty sands remains an outstanding issue since it continues to lead to catastrophic consequences in recent earthquake events. The cyclic characteristics, such as cyclic failure pattern and cyclic resistance, are the fundamental aspects of liquefaction analysis. The difficulty and uncertainty of characterization and evaluation of cyclic behavior and liquefaction resistance of silty sand mainly come from the complicated interactions of various influencing factors, such as packing density, confining pressure, initial shear stress, cyclic loading amplitude, soil properties, and soil fabric. This study presents a series of laboratory testing results to identify the cyclic failure patterns of silty sands considering different soil states, fines contents, initial static shear stress, etc. It is found that the failure patterns are related to the states of soils and the cyclic loading characteristics, i.e., the combination of initial shear stress and cyclic loading amplitude for silty sands with similar soil fabric, and that the cyclic resistance of silty sands is also a function of soil states and initial static shear stress for similar soil fabric. Critical state soil mechanics is implemented to characterize the cyclic failure patterns and cyclic resistance. Implications of the present study to the existing liquefaction assessment methods are also discussed.

Abstract 3606: HDAC8-mediated inhibition of EP300 drives a neural crest-like transcriptional state that increases melanoma brain metastasis

Abstract Melanomas are heterogeneous and adopt multiple transcriptional states that can confer an invasive phenotype and resistance to therapy. Little is known about the epigenetic drivers of these cell states, limiting our ability to regulate melanoma heterogeneity and tumor progression. Here we identify stress-induced HDAC8 activity as the driver of a neural crest stem cell (NCSC)-like transcriptional state that increased the formation of melanoma brain metastases (MBM). Exposure of melanocytes and melanoma cells to multiple different stresses led to HDAC8 activation, a switch to a NCSC gene expression signature and the adoption of an amoeboid, invasive phenotype. This cell state enhanced the survival of melanoma cells under shear stress conditions and increased the formation of metastases in the brain. Single cell RNA-seq analyses showed that HDAC8 expression was correlated with the NCSC cell state in clinical MBM specimens. ATAC-Seq and ChIP-Seq analysis showed HDAC8 to alter chromatin structure by increasing H3K27ac and accessibility at c-Jun binding sites without changing global histone acetylation. The increased accessibility of Jun binding sites was paralleled by decreased H3K27ac and accessibility at MITF binding sites and loss of melanoma-lineage gene expression. Mass spectrometry-based acetylomics demonstrated that HDAC8 deacetylated the histone acetyltransferase (HAT) EP300 leading to its enzymatic inactivation. This, in turn, led to an increased binding of EP300 to Jun-transcriptional sites and decreased binding to MITF-transcriptional sites. Increased expression of EP300 decreased invasion and increased the sensitivity of melanoma cells to multiple stresses while inhibition of EP300 function increased invasion and resistance to stress. We identified HDAC8 as a novel mediator of transcriptional co-factor inactivation and chromatin accessibility that increases MBM development. Citation Format: Michael Emmons, Richard Bennett, Alberto Riva, Chao Zhang, Robert Macaulay, Daphne Dupéré-Richér, Bin Fang, John Koomen, Jiannong Li, Ann Chen, Edward Seto, Jonathan Licht, Keiran Smalley. HDAC8-mediated inhibition of EP300 drives a neural crest-like transcriptional state that increases melanoma brain metastasis. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3606.

Abstract 3618: The role of endothelial ACKR1 in triple-negative breast cancer metastasis

Abstract Metastasis is the most common cause of breast cancer mortality. Of the breast cancer subtypes, triple-negative breast cancer (TNBC) is the deadliest due to its increased likelihood to metastasize. Tumor cell extravasation is a critical step of metastasis and allows circulating tumor cells to exit the vasculature and seed distant tissues. Clear understanding of the major regulators of tumor cell extravasation will provide insights into the progression of TNBC metastasis. One potential regulator of TNBC cell extravasation is ACKR1. In many contexts, ACKR1 expression is required in endothelial cells (EC) for leukocyte extravasation. Endothelial ACKR1 binds CXCL2, a promigratory chemokine, and localizes it to EC junctions to guide neutrophils through leukocyte extravasation. CXCL2 expression in TNBC cells is also necessary for tumor cell extravasation from lung microvasculature and for tumor metastasis. Our preliminary data show that ACKR1 is required in at least one stromal cell type for TNBC metastasis from the primary tumor to the lung. These data suggest that endothelial ACKR1-CXCL2 interactions may mediate tumor cell extravasation in TNBC metastasis. Therefore, we hypothesize that endothelial ACKR1 promotes metastasis via retention of CXCL2 at EC junctions, increasing tumor cell chemotaxis and extravasation. To address this hypothesis, we will examine the in vivo significance of endothelial ACKR1 expression using our validated ACKR1 endothelial cell-specific knockout mouse model. We will test the requirement for endothelial ACKR1 for metastasis of orthotopically implanted TNBC tumors to distant sites in the lung and for extravasation of circulating tumor cells into lung tissue. We will determine which steps of extravasation require ACKR1 by evaluating ACKR1-low and ACKR1-overexpressing ECs using an Ibidi flow co-culture system that recapitulates the shear stress conditions of pulmonary microvasculature. We will examine whether these steps are dependent on CXCL2 by introducing CXCL2-neutralizing antibodies to the Ibidi flow system and observing their effects on each extravasation step. Our proposed studies will establish the role of endothelial ACKR1 in TNBC metastatic progression and determine the specific steps of tumor cell extravasation in which endothelial ACKR1 and CXCL2 function. Understanding these processes may guide development of ACKR1 as a prognostic marker for metastasis and can provide mechanistic insight into candidate chemokine and chemokine receptor inhibitors under evaluation for treatment of breast cancer. Citation Format: Samuel Tanner Roach, Chinwe Ewenighi-Amankwah, J Dufraine, L.A. Naiche, Jan K. Kitajewski. The role of endothelial ACKR1 in triple-negative breast cancer metastasis. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3618.

Effect of Concentration on Shear Stress of Nanyang Expansive Soil Slurry

The yield stress of mud is the basis for analyzing the flow and desilting of debris flow. Nanyang expansive soil mud was used as experimental materials, and the rheological curves of mud with different solid volume concentrations were obtained by using the stress scanning rheological experiment of Antonpa mcr702 rheometer. For Nanyang expansive soil slurry with different solid volume concentration, the change process of shear stress with shear rate is analyzed. According to the experimental results, the rheological process of Nanyang expansive soil slurry is divided into three stages: stress growth, stress attenuation and stress stability. The slurry concentration has obvious influence on the dynamic and static shear stress of mud.

Shear Damage Simulations of Rock Masses Containing Fissure-Holes Using an Improved SPH Method

Fissures and holes widely exist in rock mechanics engineering, and, at present, their failure mechanisms under complex compress and shear stress states have not been well recognized. In our work, a fracture mark, ξ, is introduced, and the kernel function of the smoothed-particle hydrodynamics (SPH) is then re-written, thus realizing the fracture modelling of the rock media. Then, the numerical models containing the fissures and holes are established, and their progressive failure processes under the compress and shear stress states are simulated, with the results showing that: (1) the improved SPH method can reflect the dynamic crack propagation processes of the rock masses, and the numerical results are in good agreement with the previous experimental results. Meanwhile, the improved SPH method can get rid of the traditional mesh re-division problems, which can be well-applied to rock failure modeling; (2) the hole shapes, fissure angles, fissure lengths, fissure numbers, and confining pressure all have great impacts on the final failure modes and peak strengths of the model; and (3) in practical engineering, the rock masses are in the 3D stress state, therefore, developing a high performance 3D SPH program and applying it to engineering in practice will be of great significance.

Role of triggering receptor expressed on myeloid cells-1 in the mechanotransduction signaling pathways that link low shear stress with inflammation

This study sought to investigate the role of triggering receptor expressed on myeloid cells-1 (TREM-1) in the mechanotransduction signaling pathways that link low shear stress with inflammation. Human coronary artery endothelial cells, human coronary artery smooth muscle cells, and THP-1 monocytes were co-cultured and exposed to varying endothelial shear stress (ESS) conditions: low (5 ± 3 dynes/cm2), medium (10 ± 3 dynes/cm2), and high (15 ± 3 dynes/cm2). We showed that low ESS increased the expression of TREM-1 by the cultured cells leading to increased production of inflammatory mediators and matrix-degrading enzymes, whereas high ESS did not have a significant effect in the expression of TREM-1 and inflammatory mediators. Furthermore, TREM-1 transcriptional inhibition with siRNA in endothelial cells, smooth muscle cells, and monocytes exposed to low ESS, led to a significant reduction in the production of vascular inflammatory mediators and matrix-degrading enzymes. Additionally, we identified the transcription factors that appear to upregulate the TREM-1 gene expression in response to low ESS. To the best of our knowledge, this is the first study to investigate the pathophysiologic association and molecular pathways that link low ESS, TREM-1, and inflammation using a sophisticated in-vitro model of atherosclerosis. Future studies on animals and humans are warranted to investigate the potential of TREM-1 inhibitors as adjunctive anti-atherosclerotic therapies.

Experiment and particle flow simulation of unloading induced shearing properties for jointed sandstone materials

Shear tests on sandstone containing non-connected joints under the unloading normal stress but fixed shear stress conditions were conducted with various initial normal stress and unloading rate. During the unloading process, continuous shear dilation could be observed. For initial normal stress = 5–20 MPa, the sliding initiation normal displacement decreased by 17.14–66.32 % but the sliding initiation shear displacement increased by 21.91–98.19 %. The sliding initiation normal stress declined by 7.18–15.57 % for unloading rate = 0.002–0.01 MPa/s but increased by a factor of 3.18–3.53 with the initial normal stress. Shear sliding of the jointed samples was more prone to occur at a smaller initial normal stress with a larger unloading rate. From the numerical simulation results via PFC2D, during the unloading process, cracking was first initiated from central part of the rock bridge and propagated gradually to coalesce with cracks developed from the joint tips. The penetrating fracture surfaces led to shear sliding of the jointed samples, and shear wear occurred on the fractures as shear displacement continued increasing. For unloading rate = 0.002–20 MPa/s, quantity of cracks corresponding to shear displacement of 1.2 mm decreased by 12.18–36.34 %. Cracking degree was more serious for the samples at a low unloading rate due to progressively developed cracks near the main failure planes before shear sliding and more accumulated cracks in the shearing wear stage for fractures under a larger normal stress.

The effect of shear-dependent flocculation on the multimodality of effective particle size distributions in a gravel-bed river during high flows

PurposeMultimodal effective particle size distributions (EPSDs) develop as flocculation and particle breakage occur dynamically in a fluid shear and such distributions have been previously reported in coastal and estuarine waters to understand flocculation processes. Here, we use time varying multimodal EPSDs and hydraulic parameters (discharge and bed shear stress) to assess freshwater flocculation in a gravel-bed river in southern Alberta, Canada.MethodsInstantaneous discharge, volume concentration (VC), and EPSD of suspended solids were measured during three high discharge events at four study sites in a 10 km reach of the Crowsnest River. The EPSD and VC of suspended solids (< 500 µm) were measured in the centroid of flow with a LISST-200x. Bed shear stress for measured discharge was obtained using a flow model, MOBED.ResultsMultimodal EPSDs consisted of primary particles, flocculi, microflocs, and macroflocs. Shear dependent flocculation was consistently observed for all sites and events, due to low and high shear stress flocculation, particle breakage, and mobilization of tributary sub-catchment derived particles. Higher shear stress limited flocculation to smaller floc sizes, while lower bed shear stress conditions created higher volumes of macroflocs.ConclusionFlocculation and particle breakage processes based on relationships between particle size and hydraulic properties presented herein have implications for advancing fine sediment transport models by a variable cohesion factor as a function of floc size class.

Combined effects of initial static shear and continuous principal stress rotation on the liquefaction behavior of granular materials

Extensive efforts have been made separately to investigate the effects of the initial static shear and the continuous principal stress rotation on the liquefaction behavior of sandy soils. However, the interplay and the combined effects of these two important factors have not been adequently studied, which may simply be attributed to the difficulty of implementing such complex loading path in the labrotory tests without introducing severe stress consentration. In this study, a newly-developed discrete element approach that avoids stress concentration and can apply arbitrary undrained loading path was implemented. Based on this approach, the cyclic resistance of both inherently anisotropic and isotropic granular materials under continuous principal stress rotation were studied considering different initial static shear levels. The internal structure evolution of the specimens were quantified through a contact-normal-based fabric tensor that describes the load-bearing structure of the granular specimens. The simulation results show that under otherwise identical condition, the specimen with higher initial static shear stress level is more prone to plastic shear strain accumulation and has weaker cyclic resistance under continuous principal stress rotation, which can be well explained by the fabric evolution.

Evaluation of static responses for layered composite arches

Abstract Layered composite materials are widely used across a variety of sectors, including the automotive industry, aerospace engineering, offshore, and various mechanical domains, because of their strong yet lightweight structures. Therefore, various emergent theories are available on the deformation of layered beams. The previous research studies are insufficient as they are based on deformation of layered composite and sandwich arches with simply supported (SS) end conditions. Therefore, it is a good opportunity for researchers to investigate the arches using exponential shear deformation and normal deformation theory. The leading hypothesis mainly adds to the research of bending for sandwich and layered composite arches adopting the exponential theory. The present theory does not require any shear correction factor to satisfy zero transverse shear stress condition at the bottom and top fibers of arches. Governing equations and associated end conditions are derived through principle of virtual work. Navier’s techniques used for sandwich and layered composite arches are SS boundary conditions subjected to uniformly distributed load. The results of the current study showed that the exponential normal and shear deformation theories may be used to evaluate static responses for layered composite and sandwich arches. The obtained results from the present theory are validated through the results available in published literature.

Natural Frequencies of Soft-Core Composite Sandwich Plates Reinforced by FG Graphene Platelets

Superior mechanical properties such as ultrahigh stiffness and strength along with amazing physical properties have led to the use of nano graphene platelets (GPLs) as a reinforcement in polymer composite structures such as sandwich plates. In this research, free vibration analysis of rectangular sandwich plates with functionally graded (FG) GPL-reinforced face sheets and a soft core is investigated. A high-order three-layer accurate sandwich plate theory is employed to model the soft-core sandwich structure. Third order shear deformable plate theory is applied for functionally graded graphene nano-composite face sheets while second and third order polynomials are used for displacements of the soft core. In this theory, the transverse flexibility of the core as well as the continuity conditions of transverse shear stresses are assumed. Hamilton’s principle is applied to extract the motion equations and the corresponding analytical solutions are presented. The effects of materials properties and geometrical parameters on the natural frequencies of the sandwich plates are studied. A comparison of the present results in particular cases with those of published studies confirms the accuracy of the present analysis. Results show that the natural frequencies of the sandwich plates are enhanced by reinforcing the face sheets with GPLs.

Cyclic mobility following plastic strain accumulation in sand

Hybrid failure modes/mechanisms under non-symmetrical cyclic loading (partial stress reversal) have received little attention. This paper presents data from cyclic undrained triaxial tests of sand, with initial static shear stress, to investigate a hybrid failure mechanism that controls the behaviour of dilative sand in a narrow range of non-symmetric stress reversal levels. The results indicate that cyclic mobility (Mob) can emerge following significant accumulation of plastic strain. Transition from plastic strain accumulation to Mob can be associated with the instability and phase transformation lines in extension. Two different types of transition and the mechanisms involved are discussed.

A refined stiffened plate element for stability analysis of the stiffened sandwich structures

The stiffened plate is one type of representative thin-walled structure consisting of plates reinforced by varying stiffeners to strengthen its resistance to buckling deformation. However, the mechanism by which stiffeners could efficiently improve the buckling resistance of such structures has not been well reported. Therefore, to investigate the mechanism and predict buckling behavior of the structures accurately, a refined Reddy-type global-local stiffened plate model (RGLS) is established firstly, which can fulfill continuous condition of in-plane displacement and transverse shear stress among the adjacent layers between the plate and stiffeners. In conjunction with the established model, a refined triangular element which can accommodate the continuous condition of transverse shear stresses at the interface between the plate and the stiffeners is constructed. Subsequently, on the basis of the unidirectional stiffened sandwich structures, the symmetric grid-stiffened sandwich structures are proposed. The buckling behavior of grid-stiffened structures is further explored by the present element. A full experimental study and a system of numerical examples have been performed to assess the reliability of the established elements. These examples demonstrate that the buckling loads could be captured by the developed element with high computational efficiency and accuracy. However, the models will significantly overrate buckling loads of the stiffened sandwich structures without considering the continuum condition of the transverse stresses at the plate-stiffener interface.

Shear Stress Markedly Alters the Proteomic Response to Hypoxia in Human Pulmonary Endothelial Cells.

Blood flow produces shear stress that homeostatically regulates the phenotype of pulmonary endothelial cells, exerting antiinflammatory and antithrombotic actions and maintaining normal barrier function. Hypoxia due to diseases, such as chronic obstructive pulmonary disease (COPD), causes vasoconstriction, increased vascular resistance, and pulmonary hypertension. Hypoxia-induced changes in endothelial function play a central role in the development of pulmonary hypertension. However, the interactive effects of hypoxia and shear stress on the pulmonary endothelial phenotype have not been studied. Human pulmonary microvascular endothelial cells were cultured in normoxia or hypoxia while subjected to physiological shear stress or in static conditions. Unbiased proteomics was used to identify hypoxia-induced changes in protein expression. Using publicly available single-cell RNA sequencing datasets, differences in gene expression between the alveolar endothelial cells from COPD and healthy lungs were identified. Sixty proteins were identified whose expression changed in response to hypoxia in conditions of physiological shear stress but not in static conditions. These included proteins that are crucial for endothelial homeostasis (e.g., JAM-A [junctional adhesion molecule A], ERG [ETS transcription factor ERG]) or implicated in pulmonary hypertension (e.g., thrombospondin-1). Fifty-five of these 60 have not been previously implicated in the development of hypoxic lung diseases. mRNA for 5 of the 60 (ERG, MCRIP1 [MAPK regulated corepressor interacting protein 1], EIF4A2 [eukaryotic translation initiation factor 4A2], HSP90AA1 [heat shock protein 90 alpha family class A member 1], and DNAJA1 [DnaJ Hsp40 (heat shock protein family) member A1]) showed similar changes in the alveolar endothelial cells of COPD compared with healthy lungs in females but not in males. These data show that the proteomic responses of the pulmonary microvascular endothelium to hypoxia are significantly altered by shear stress and suggest that these shear-hypoxia interactions are important in the development of hypoxic pulmonary vascular disease.

Analysis of the Shear Stress State at the Native Material/Repair Material Interface in Cooling Towers

The structure of the cooling tower shell locally made of materials differing in their specifications is considered. Such structures are created as a result of cooling tower shell repairs or reinforcements. The results of an analysis of the distribution and magnitude of shear stress at the interface between layers made of different materials are presented.

Influence of Consolidation Shear Stress Magnitude and Orientation on Stress–Strain Behavior of Sand under Traffic Loading

Traffic-load-induced principal stress rotation has been widely studied. Most of these tests were conducted using a hollow cylinder apparatus to simulate ground-level traffic conditions, and only one shear direction was considered. However, fewer studies focused on the stress conditions of subsoil below slopes or embankments, which involved bidirectional shear. In this study, a series of bidirectional cyclic simple shear tests were performed using the variable-direction dynamic cyclic simple shear apparatus to investigate the effects of multidirectional principal stress rotation on sand induced by traffic loads. The specimens were first Kα-consolidated with various static shear stresses and then subjected to traffic-induced cyclic shearing in different directions. The experimental results showed that both the magnitude and orientation of the initial static shear stress had significant effects on sand strain development and stress–strain behavior. A larger angle led to a higher axial strain, while larger initial shear stresses led to stiffer sand responses.