The purpose of the present study was to assess the bonding capacity and efficacy of acrylic teeth to denture bases following two different chemical surface treatments. A two-metal mold measuring 35 mm in length and 12 mm in diameter was created specifically for the investigation in order to standardize the wax pattern-based tooth attachment at 45°. Following standard protocol, 75 wax cylinder specimens were flasked, dewaxed, and surface treatment of teeth was done as follows with 25 samples in each group-group I: control group, group II: monomethyl methacrylate monomer group, group III: acetone group. The curing process was completed following the packing of the denture base material. The samples' shear bond strength was assessed using a universal testing machine. Every sample was taken out when it fractured, and the shear load (Newton, N) was noted. The significance of the variation in applied shear load was assessed using one-way analysis of variance (ANOVA) and post hoc ANOVA Tukey's honestly significant difference (HSD) test at the 5% level of significance. The maximum shear bond strength was found in the samples treated with acetone (183.21 ± 0.06) followed by samples treated with monomethyl methacrylate monomer (171.64 ± 0.12) and the control group (149.32 ± 0.04). A statistically significant difference was found between the different groups (p < 0.001). In conclusion, according to the current study's findings, acetone chemical surface treatment of acrylic teeth produced the strongest bond when compared with the control group and monomethyl methacrylate monomer. In prosthodontic practice, artificial teeth regularly de-bond and separate from the denture base. A weak interface is produced when certain clinical conditions, such as ridge prominence, cause excessive cutting of the acrylic teeth and base. Where the denture base polymer meets the teeth's highly cross-linked matrix, it de-bonds adhesively. Therefore, the bonding between the acrylic teeth and the denture base material can be improved by the chemical surface treatment. How to cite this article: Chaudhuri NG, Lahiri B, Francis NT, et al. Evaluation of the Bond Strength of Acrylic Teeth to Denture Base after Various Chemical Surface Treatments: An In Vitro Study. J Contemp Dent Pract 2024;25(6):514-517.