Sharp Tip Research Articles

Quantitative Field Emission Imaging for Studying the Doping-Dependent Emission Behavior of Silicon Field Emitter Arrays.

Field emitter arrays (FEAs) are a promising component for novel vacuum micro- and nanoelectronic devices, such as microwave power amplifiers or fast-switching X-ray sources. However, the interrelated mechanisms responsible for FEA degradation and failure are not fully understood. Therefore, we present a measurement method for quantitative observation of individual emission sites during integral operation using a low-cost, commercially available CMOS imaging sensor. The emission and degradation behavior of three differently doped FEAs is investigated in current-regulated operation. The measurements reveal that the limited current of the p-doped emitters leads to an activation of up to 55% of the individual tips in the array, while the activation of the n-type FEA stopped at around 30%. This enhanced activation results in a more continuous and uniform current distribution for the p-type FEA. An analysis of the individual emitter characteristics before and after a constant current measurement provides novel perspectives on degradation behavior. A burn-in process that trims the emitting tips to an integral current-specific ideal field enhancement factor is observed. In this process, blunt tips are sharpened while sharp tips are dulled, resulting in homogenization within the FEA. The methodology is described in detail, making it easily adaptable for other groups to apply in the further development of promising FEAs.

A Quasi-2D Exploration of Mixed-Mode Fracture Propagation in Concrete Semi-Circular Chevron-Notched Disks

Most semi-circular bend (SCB) tests on concrete have been conducted with a pre-crack with a straight-through tip, thereby undermining the determination of the tensile fracture toughness (KIc). Therefore, the present study involved mixed-mode (tensile–shearing) fracture propagation in concrete semi-circular chevron-notched disks (i.e., with a sharp notch tip) using SCB tests and the FRANC2D numerical simulation software. The inclined notch angle (β) was varied from 0° to 70° while the other settings remained fixed, and the crack mouth opening displacement (CMOD) of the notch was measured constantly. The stress distribution was analyzed using finite-element simulations, and the experimental results showed that this testing method was robust. The maximum failure load and the fracture propagation angle increased with β, and wing fracture was observed. With FRANC2D simulating these SCB tests successfully, it was found that the tensile stress concentration around the notch tip moved toward the upper face of the notch, and the compressive stress concentration formed on the notch tip. The tensile mode was generated as the CMOD kept increasing for β = 0–30°, whereas the mixed mode became more evident as the CMOD kept decreasing for β = 45–70°. The fracture process zone was found for β = 0–30° but not for β = 45–70°. This mixed-mode fracture is predicted better by the criterion of extended maximum tangential strain than by other criteria, and there is a linear relationship between CMOD and KIc, as examined previously for pavement and concrete materials.

Inferring mechanical properties of the SARS-CoV-2 virus particle with nano-indentation tests and numerical simulations

The pandemic caused by the SARS-CoV-2 virus has claimed more than 6.5 million lives worldwide. This global challenge has led to accelerated development of highly effective vaccines tied to their ability to elicit a sustained immune response. While numerous studies have focused primarily on the spike (S) protein, less is known about the interior of the virus. Here we propose a methodology that combines several experimental and simulation techniques to elucidate the internal structure and mechanical properties of the SARS-CoV-2 virus. The mechanical response of the virus was analyzed by nanoindentation tests using a novel flat indenter and evaluated in comparison to a conventional sharp tip indentation. The elastic properties of the viral membrane were estimated by analytical solutions, molecular dynamics (MD) simulations on a membrane patch and by a 3D Finite Element (FE)-beam model of the virion's spike protein and membrane molecular structure. The FE-based inverse engineering approach provided a reasonable reproduction of the mechanical response of the virus from the sharp tip indentation and was successfully verified against the flat tip indentation results. The elastic modulus of the viral membrane was estimated in the range of 7–20 MPa. MD simulations showed that the presence of proteins significantly reduces the fracture strength of the membrane patch. However, FE simulations revealed an overall high fracture strength of the virus, with a mechanical behavior similar to the highly ductile behavior of engineering metallic materials. The failure mechanics of the membrane during sharp tip indentation includes progressive damage combined with localized collapse of the membrane due to severe bending. Furthermore, the results support the hypothesis of a close association of the long membrane proteins (M) with membrane-bound hexagonally packed ribonucleoproteins (RNPs). Beyond improved understanding of coronavirus structure, the present findings offer a knowledge base for the development of novel prevention and treatment methods that are independent of the immune system.

Multifunctional Microneedle Patches via Direct Ink Drawing of Nanocomposite Inks for Personalized Transdermal Drug Delivery.

Additive manufacturing, commonly known as 3D printing, allows decentralized drug fabrication of orally administered tablets. Microneedles are comparatively favorable for self-administered transdermal drug delivery with improved absorption and bioavailability. Due to the cross-scale geometric characteristics, 3D-printed microneedles face a significant trade-off between the feature resolution and production speed in conventional layer-wise deposition sequences. In this study, we introduce an economical and scalable direct ink drawing strategy to create drug-loaded microneedles. A freestanding microneedle is efficiently generated upon each pneumatic extrusion and controlled drawing process. Sharp tips of ∼5 μm are formed with submillimeter nozzles, representing 2 orders of magnitude improved resolution. As the key enabler of this fabrication strategy, the yield-stress fluid inks are formulated by simply filling silica nanoparticles into regular polymer solutions. The approach is compatible with various microneedles based on dissolvable, biodegradable, and nondegradable polymers. Various matrices are readily adopted to adjust the release behaviors of the drug-loaded microneedles. Successful fabrication of multifunctional patches with heterogeneously integrated microneedles allows the treatment of melanoma via synergistic photothermal therapy and combination chemotherapy. The personalized patches are designed for cancer severity to achieve high therapeutic efficacy with minimal side effects. The direct ink drawing reported here provides a facile and low-cost fabrication strategy for multifunctional microneedle patches for self-administering transdermal drug delivery.

Pathology caused by introduced Neoergasilus japonicus (Copepoda: Ergasilidae) to the skin of indigenous Tilapia sparrmanii in South Africa and scanning electron microscopy study of wound-inflicting structures.

Neoergasilus japonicus, a fish ectoparasite indigenous to eastern and southern Asia, has been introduced to various regions, including Europe and North and South America, where it spreads at an alarming rate. The parasite is not host-specific. It was also collected from the Vaal-Orange and Limpopo river systems in South Africa. This report describes the morphology of the wound-inflecting structures in N. japonicus and relates their morphology to the pathological effect on Tilapia sparrmanii. Host tissue with parasites attached was imbedded in resin for histological sectioning and stained with haematoxylin and eosin. Additional specimens were prepared for scanning electron microscopy. Adult female parasites attach by inserting the hooked tips of the antennae into the epidermis. The maxillulae are minute but have sharp tips. The maxillae have sharp tips on the endopod and brush-like setae on the exopod. The mandibles are armed with sharp tips. These structures are used to remove the epidermis of the host and brush it into the buccal cavity. Host tissue in the intestine confirms that adult females consume host tissue leaving the dermis exposed to opportunistic diseases. The microscopic size of the parasite prevents casual observation, and its occurrence in Africa is, therefore, probably underreported.

A Planar-Type Micro-Biopsy Tool for a Capsule-Type Endoscope Using a One-Step Nickel Electroplating Process.

Millimeter-scale biopsy tools combined with an endoscope instrument have been widely used for minimal invasive surgery and medical diagnosis. Recently, a capsule-type endoscope was developed, which requires micromachining to fabricate micro-scale biopsy tools that have a sharp tip and other complex features, e.g., nanometer-scale end-tip sharpness and a complex scalpel design. However, conventional machining approaches are not cost-effective for mass production and cannot fabricate the micrometer-scale features needed for biopsy tools. Here, we demonstrate an electroplated nickel micro-biopsy tool which features a planar shape and is suitable to be equipped with a capsule-type endoscope. Planar-type micro-biopsy tools are designed, fabricated, and evaluated through in vitro tissue dissection experiments. Various micro-biopsy tools with a long shaft and sharp tip can be easily fabricated using a thick photoresist (SU8) mold via a simple one-step lithography and nickel electroplating process. The characteristics of various micro-biopsy tool design features, including a tip taper angle, different tool geometries, and a cutting scalpel, are evaluated for efficient tissue extraction from mice intestine. These fabricated biopsy tools have shown appropriate strength and sharpness with a sufficient amount of tissue extraction for clinical applications, e.g., cancer tissue biopsy. These micro-scale biopsy tools could be easily integrated with a capsule-type endoscope and conventional forceps.

Polarization Sensitivity in Scattering-Type Scanning Near-Field Optical Microscopy—Towards Nanoellipsometry

Electric field enhancement mediated through sharp tips in scattering-type scanning near-field optical microscopy (s-SNOM) enables optical material analysis down to the 10-nm length scale and even below. Nevertheless, the out-of-plane electric field component is primarily considered here due to the lightning rod effect of the elongated s-SNOM tip being orders of magnitude stronger than any in-plane field component. Nonetheless, the fundamental understanding of resonantly excited near-field coupled systems clearly allows us to take profit from all vectorial components, especially from the in-plane ones. In this paper, we theoretically and experimentally explore how the linear polarization control of both near-field illumination and detection can constructively be implemented to (non-)resonantly couple to selected sample permittivity tensor components, e.g., explicitly to the in-plane directions as well. When applying the point-dipole model, we show that resonantly excited samples respond with a strong near-field signal to all linear polarization angles. We then experimentally investigate the polarization-dependent responses for both non-resonant (Au) and phonon-resonant (3C-SiC) sample excitations at a 10.6 µm and 10.7 µm incident wavelength using a tabletop CO2 laser. Varying the illumination polarization angle thus allows one to quantitatively compare the scattered near-field signatures for the two wavelengths. Finally, we compare our experimental data to simulation results and thus gain a fundamental understanding of the polarization’s influence on the near-field interaction. As a result, the near-field components parallel and perpendicular to the sample surface can be easily disentangled and quantified through their polarization signatures, connecting them directly to the sample’s local permittivity.

Effect of different manual puncture methods on donkey embryo before vitrification

The application of embryo recovery and transfer technology in the donkey industry is far lower than that of horses and cattle. Sometimes the recovered embryos could not be transferred in time, which required embryo cryopreservation. The embryo cryopreservation technology is more conducive to the preservation and transportation of recovered embryos with excellent genetic traits. However, this technique for donkey embryos is not efficient and needs further optimization. The objective of this study was to evaluate the effect of different manual puncture methods on the viability and pregnancy rates of vitrified-thawed donkey embryos. A total of 117 donkey embryos were recovered on day 7–8 post-ovulation and were divided into four groups by random assortment. There were 28 embryos without puncture or cryopreservation (Control). 58 embryos were manually punctured using a 29G needle (VG, n = 29) or microneedle with a sharp tip of <10 μm (VM, n = 29), then vitrified in 15% EG + 15% DMSO + 0.5 M sucrose. Another 31 embryos were punctured with a microneedle and vitrified with 10% EG + 10% DMSO +0.5 M sucrose +2 mol/L proline (VMP). Both fresh embryos and vitrified-thawed embryos were incubated for 3 h (38.5 °C, 5% CO2 in air) before embryo transfer. The results showed that the embryo recovery rates on day 7.5 and 8 were higher than day 7 (P < 0.05). After incubation, dead cells were assessed and the percentages of dead cells in VM and VMP were lower than that in VG (P < 0.05), although both were higher than those in Control (P < 0.05). The pregnancy rates after 23 days post transfer were assessed and the results showed that the pregnancy rate in VG (8.0%) was lower than that in Control (41.7%), VM (24.0%) and VMP (29.6%) (P < 0.05). No pregnancies resulted from the 10 embryos with diameters ≤650 μm in VG, which lower than either VM (33.3%) or VMP (38.9%) (P < 0.05). While, there was no difference in pregnancy rates among all vitrification groups when embryos were >650 μm in diameter (P > 0.05). In conclusion, the embryo recovery rate on day 7 after ovulation was relatively low, and it was more appropriate to extend it to day 8. Microneedle puncture could reduce embryo damage and achieve a higher pregnancy rate compared with 29G needles. Proline has the potential to improve donkey embryo cryopreservation.

The Rise of Structurally Anisotropic Plasmonic Janus Gold Nanostars.

Nanostructures intrinsically possessing two different structural or functional features, often called Janus nanoparticles, are emerging as a potential material for sensing, catalysis, and biomedical applications. Herein, we report the synthesis of plasmonic gold Janus nanostars (NSs) possessing a smooth concave pentagonal morphology with sharp tips and edges on one side and, contrastingly, a crumbled morphology on the other. The methodology reported herein for their synthesis - a single-step growth reaction - is different from any other Janus nanoparticle preparation involving either template-assisted growth or a masking technique. Interestingly, the coexistence of lower- and higher-index facets was found in these Janus NSs. The general paradigm for synthesizing gold Janus NSs was investigated by understanding the kinetic control mechanism with the combinatorial effect of all the reagents responsible for the structure. The optical properties of the Janus NSs were realized by corelating their extinction spectra with the simulated data. The size-dependent surface-enhanced Raman scattering (SERS) activity of these Janus NSs was studied with 1,4-BDT as the model analyte. Finite-difference time-domain simulations for differently sized particles revealed the distribution of electromagnetic hot-spots over the particles resulting in enhancement of the SERS signal in a size-dependent manner.

Plasmonic Dodecahedral-Walled Elongated Nanoframes for Surface-Enhanced Raman Spectroscopy.

Here, elongated pseudohollow nanoframes composed of four rectangular plates enclosing the sides and two open-frame ends with four ridges pointing at the tips for near-field focusing are reported. The side facets act as light-collecting domains and transfer the collected light to the sharp tips for near-field focusing. The nanoframes are hollow inside, allowing the gaseous analyte to penetrate through the entire architecture and enabling efficient detection of gaseous analytes when combined with Raman spectroscopy. The resulting nanostructures are named Au dodecahedral-walled nanoframes. Synthesis of the nanoframes involves shape transformation of Au nanorods with round tips to produce Au-elongated dodecahedra, followed by facet-selective Pt growth, etching of the inner Au, and regrowth steps. The close-packed assembly of Au dodecahedral-walled nanoframes exhibits an attomolar limit of detection toward benzenethiol. This significant enhancement in SERS is attributed to the presence of a flat solid terrace for a large surface area, sharp edges and vertices for strong electromagnetic near-field collection, and open frames for effective analyte transport and capture. Moreover, nanoframes are applied to detect chemical warfare agents, specifically mustard gas simulants, and 20 times higher sensitivity is achieved compared to their solid counterparts.

Interlaminar Response of LSI-Produced C/SiC Ceramic Matrix Composites: Experiments and Modelling

This work investigates the interlaminar properties of a C/SiC composite produced by Liquid Silicon Infiltration, combining experiments based on Double Cantilever Beam tests and numerical analyses. Experimentally, a method to obtain pre-cracks with sharp tips at precise locations is proposed, and specimens with different thickness are used to investigate the effects of bending stress states in the delamination process. The properties of tri-linear Cohesive Zone Models for the modelling of delamination are identified numerically, by using automatic regression techniques without requiring additional assumptions or testing. Fiber bridging effects were observed and modelled, including the evaluation of the process zone lengths with different experimental, analytical, and numerical methods. Overall, the work provides a qualitative insight in the delamination process of long fiber reinforced C/SiC laminates produced with a cost-affordable technique and proposes an experimental and numerical protocol to characterize and model delamination phenomena, taking into account the scattering of material properties.

The effects of impactor shape on the compression after impact strength of carbon/epoxy face sheet foam core sandwich structure

This study presents experimental results of compression after impact (CAI) strength testing of foam core sandwich structure with carbon/epoxy face sheets impacted at three different energies with either a blunt or sharp tip impactor. The impact energies used were chosen to span the barely visible impact damage (BVID) thresholds for the sharp (4.1 J) and blunt (12.9 J) impactors with an impact energy approximately halfway between these (8.1 J) also used. While most impact testing on composites utilize a hemispherical (blunt) impactor, actual damage to a part may be due to an object impacting the part that is not blunt, but sharp and the impact response and resulting CAI strength values may be different for a given impact energy level. In this study, with regards to the impact response, the sandwich specimens showed larger transverse displacements (by about a factor of two) during the impact event when impacted by a sharp impactor versus a blunt impactor. The maximum load of impact was larger for the blunt impactor by about a factor of three. The barely visible impact damage (BVID) threshold energy was lower by about a factor of three for the sharp impactor. The absorbed energy of impact was higher for the sharp impactor. The CAI strength results showed that the sharp impactor gave lower average CAI strength values at the lowest impact energy level used, slightly lower average CAI strength values for the medium impact energy used and about the same average CAI strength values for the highest impact energy used.

Promote Stable Lithium Deposition by Curvature Optimization Using Lithiophilic Ni Nano‐starfish@Al Structured Current Collector

AbstractLithium(Li) metal is an anode candidate for rechargeable batteries due to its ultra‐high theoretical capacity and extremely low standard potential. However, the dendritic Li and dead Li have hindered the practical application of next‐generation batteries. Constructing 3D anode current collectors can not only decrease the local current density but also relieve the volume expansion. Herein, the 3D Ni nano‐starfish@Al(NSA) is synthesized as anode current collectors. Compared with the 3D Ni nano‐cone@Al(NCA) with sharp cone tips, the NSA shows a superior cycle performance, especially at high current density. This is because the NSA has a more uniform surface curvature than NCA, which makes the electric field distribution more homogeneous and reduces the local electric field, thereby inducing the even deposition of Li and suppressing the growth of Li dendrites. The NSA@Li anode gives superior cycling performance over 2800 h at a current density of 4 mA cm−2 in a symmetric cell. A full cell paired with LiFePO4 cathode shows high Coulombic efficiency of 96.8% approaching 200 cycle‐life at 0.5 °C. This work provides a view of the microstructure design of current collectors for achieving better performance Li metal batteries.

Role of Flexible Bronchoscopy using Biopsy Forceps as the Initial Attempt for Headscarf Pin Aspiration Extraction.

Flexible bronchoscopy is a less invasive procedure for extracting foreign bodies from the airways. However, studies on the extraction of headscarf pins are still very limited to determine the efficacy and safety of headscarf pin extraction using flexible bronchoscopy with biopsy forceps. This retrospective study was conducted at Persahabatan Hospital, Jakarta, Indonesia, on patients who had been treated in this hospital for headscarf pin extraction between January 2013 and February 2023. Fibreoptic bronchoscopy was performed under general anaesthesia. The pin was removed using Radial Jaw 4 mm single-use pulmonary biopsy forceps. The impacted sharp tip of the pin was freed first, and the proximal part of the pin body was gripped using biopsy forceps. Once a firm hold of the sharp end or the proximal part of the pin was secured, the bronchoscope and forceps were both slowly withdrawn under direct vision. Thirty-two cases with headscarf pin aspiration were managed by fibreoptic bronchoscopy. A total of 12 patients (37.5%) came without any respiratory complaints; however, an equal number complained of cough and 6 cases (18.7%) of haemoptysis. All the cases in which the pins were visible in the airway were found with the round head down and the sharp tip oriented superiorly in the airway and impacted in the mucosa. Fibreoptic bronchoscopy extraction succeeded in 31 cases (96.8%). Only one case was converted to surgery. There were no major complications. Fibreoptic bronchoscopy with biopsy forceps under general anaesthesia is safe and effective for the removal of headscarf pin aspiration.

Reducing Screening Effect in Close-Packed Si Field Emitter Arrays

Electric field screening effect in close-packed semiconductor field emitter arrays (FEAs) changes the current distribution among emitter tips, such that those on the edges of the array emit much higher current than those in the middle, leading to poor reliability characteristics. Using analysis, numerical simulations, and experimental verification, three different techniques to suppress electric field screening and achieve uniform current distribution are proposed. These techniques are: 1) utilizing very sharp emitter tips; 2) reducing the anode–cathode distance (ACD) to be in the order of emitter pitch; and 3) using extremely low-doped but long field emitter tips. By adopting these techniques together, a more uniform emission from the array and a high emission current density can be achieved. FEAs with 160 K emitter tips with different ACDs and emitter sharpness were fabricated using electron beam lithography. In an array with sharp emitter tips, reliable emission with a maximum current of 0.7 mA (4.4 nA/tip) and a maximum current density of 1.75 A/cm <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$_{\text{2}}$</tex-math> </inline-formula> was achieved.

A Next-Generation qPlus-Sensor-Based AFM Setup: Resolving Archaeal S-Layer Protein Structures in Air and Liquid.

Surface-layer (S-layer) proteins form the outermost envelope in many bacteria and most archaea and arrange in two-dimensional quasicrystalline structures via self-assembly. We investigated S-layer proteins extracted from the archaeon Pyrobaculum aerophilium with a qPlus sensor-based atomic force microscope (AFM) in both liquid and ambient conditions and compared it to transmission electron microscopy (TEM) images under vacuum conditions. For AFM scanning, a next-generation liquid cell and a new protocol for creating long and sharp sapphire tips was introduced. Initial AFM images showed only layers of residual detergent molecules (sodium dodecyl sulfate, SDS), which are used to isolate the S-layer proteins from the cells. SDS was not visible in the TEM images, requiring more thorough sample preparation for AFM measurements. These improvements allowed us to resolve the crystallike structure of the S-layer samples with frequency-modulation AFM in both air and liquid.

Lower hardness than strength: The auxetic composite microstructure of limpet tooth.

The limpet tooth is widely recognized as nature's strongest material, with reported strength values up to 6.5 GPa. Recently, microscale auxeticity has been discovered in the leading part of the tooth, providing a possible explanation for this extreme strength. Utilizing micromechanical experiments, we find hardness values in nanoindentation that are lower than the respective strength observed in micropillar compression tests. Using micromechanical modeling, we show that this unique behavior is a result of local tensile strains during indentation, originating from the microscale auxeticity. As the limpet tooth lacks ductility, these tensile strains lead to microdamage in the auxetic regions of the microstructure. Consequently, indentation with a sharp indenter always probes a damaged version of the material, explaining the lower hardness and modulus values gained from nanoindentation. Micropillar tests were found to be mostly insensitive to such microdamage due to the lower applied strain and are therefore the suggested method for characterizing auxetic nanocomposites. STATEMENT OF SIGNIFICANCE: This work explores the micromechanical properties of limpet teeth, nature's strongest biomaterial, using micropillar compression testing and nanoindentation. The limpet tooth microstructure consists of ceramic nanorods embedded in a matrix of amorphous SiO2 and arranged in a pattern that leads to local auxetic behavior. We report lower values for nanoindentation hardness than for compressive strength, a unique behavior usually not achievable in conventional materials. Utilizing micromechanical finite element simulations, we identify the reason for this behavior to be microdamage formation resultant of the auxetic behavior, sharp indenter tip and lack of ductility of the limpet tooth microstructure. This formation of microdamage is not expected in micropillar compression tests due to lower locally imposed strain.

Electrochemical Etching Method to Fabricate Tips for Scanning Tunnelling Microscopes using Tungsten

The goal of this project was to create a tungsten tip for use in STM machines. This project started with the research of existing methods for creating STM tips through electrochemical etching. During initial testing, the electrolyte solution was identified as a powerful variable. Once a suitable solution had been found and optimized, we made changes to the etching procedure while observing the difference between outcomes. In this way we developed a method, which could create consistently sharp tips. The focus moved from finding a method for constant power etching to pulse etching. The advantage of constant power etching was that it gave a good indication of how sharp the tips could get while being very fast, allowing for rapid testing of variables and their impact on the outcome. The advantage of pulsed etching was the ability to control how sharp the tips created were. The final procedure was to pass 4V through the equipment (see figure 9), pulsed on for 1 second and off for 8 seconds. When the wire breaks the voltage is to be turned off and the tip retrieved. Excellent tips have been produced using this method, as evidenced in this report.

Friction of graphene oxide with water nanodroplets under high relative humidity

Tribological properties of graphene oxide (GO) in high-humidity environments are important for its application as a solid lubricant. Topography, friction, and adhesion of GO with water nanodroplet are investigated at high relative humidity using atomic force microscopy. The friction of GO inside the nanodroplet depends on the tip radius. For a sharp tip, the friction of GO inside the nanodroplet is higher than that outside because the resisting force of the nanodroplet to the tip is greater than the assisting force due to the mildly decreased adhesion. For a blunt tip, the significantly decreased adhesion inside the nanodroplet results in much lower friction inside the nanodroplet than that outside. These studies enhance the understanding of the friction behaviors of GO.

How to Choose the Right AFM Probe for Your Experiment

Abstract Atomic force microscopes (AFMs) have emerged as the principal enabling tool for nanotechnology research. They are used ubiquitously in a wide range of fields: from 2D materials, semiconductors, ferroelectrics, and batteries to biomolecules, polymers, and cell biology. As the name implies, AFMs are microscopes. However, rather than using focused light or electrons to magnify sample features, AFMs scan a mechanical probe with a very sharp tip over the surface to create a high-resolution 3D topographical image. Further, by modifying the probe composition or structure, other material properties (electrical, mechanical, magnetic, etc.) can be simultaneously measured and mapped onto the topographic image for precise structure/property correlation. Clearly, the probe is key to unlocking the power of the AFM, thus, choosing the right probe is critical. In this article, we will provide novice and experienced users with basic information and guidelines to simplify the AFM probe selection process.