Introduction: Nowadays, most people are suffering from dandruff which when not treated becomes flaky and causes major damage to the scalp of a person. Dandruff is a common scalp illness caused by the yeast Pittosporum. It is not possible to completely eradicate dandruff; it can only be appropriately handled. Methods: When used as indicated, a shampoo's surfactant, often referred to as a surface-active agent, in its proper form—liquid, solid, or powder—will safely remove skin fragments, dirt, and surface oil from the scalp and hair shaft. Numerous hair care solutions use a variety of anti-fungal substances to treat dandruff. Many adverse reactions to these products have been reported, includ-ing hair loss, heightened headache, nausea, scaling, itching, and irritation. Results: Thus, an attempt was undertaken to develop a herbal anti-dandruff shampoo form safe and more successful in treating the dandruff problem by using a marketed herbal tincture. The anti-dandruff formulation was prepared by combining natural polyherbal tincture, and these tincture shows the different medicinal effects on hair. Conclusion: A number of parameters, including visual observation, pH, consistency, particle per-centage, filth dispersion, surface phenomena, and foaming content were used to assess the shampoo formulations. Also, the estimation of an anti-microbial activity by using the inoculation method. The greatest zone of inhibition demonstrated a strong antimicrobial effect for the formulation.