The present study aims to assess slope stability and landslide susceptibility mapping of road-cut slopes along Mussoorie road in the Lesser Himalayan region. A total of 18 suspected unstable slope sites were selected for the investigation, and performed geo-mechanical classification techniques, including Rock Mass Rating (RMR), Slope Mass Rating (SMR), Geological Strength Index (GSI), and kinematic analysis. For the Landslide susceptibility mapping, the Frequency Ratio (FR) method was employed using the weightage of various causative factors which includes slope, aspect, curvature, elevation, distance from streams, distance from lineaments, lithology, and rainfall. The finding indicates that out of 18 selected slopes, 4 slopes are bad slope or unstable, which includes slope 3,4 and 6 in the lower part of the Mussoorie area near Jharipani, while slope 10 near Hathi Paon-Mussoorie Road is also unstable. The slopes around Junu waterfall are stable. Partially unstable slopes may vulnerable to slope failure in the future due to heavy rainfall and unstructured construction. Additionally, the Area Under Curve (AUC) and predictive rate curve values are 61% and 78% respectively, indicating acceptable overall accuracy. This study highlights the landslide issues in Mussoorie region due to rapid urbanization & climate change and demonstrates the effectiveness of the employed methods for future risk analysis.