Shade Trees Research Articles

Shade tree trait diversity and functions in agroforestry systems: A review of which traits matter

Abstract Shade trees in agroforestry systems confer ecosystem services, such as enhanced soil fertility from diverse litter inputs, microclimate regulation via shade, and disease mitigation through trophic and abiotic interactions. With this thriving role of agroforestry in sustainable agriculture, particularly for tree crops, systematic and reliable methods to select shade trees for specific agroecosystem outcomes are crucial. Plant functional traits offer a framework to describe, select and manage shade trees. Over the last decade, shade tree leaf functional traits and whole plant traits have been assessed in agroforestry systems. Yet, we lack amalgamated information on (i) what we know about shade tree trait relationships with functions to achieve desired agroecosystem outcomes, (ii) how decades of shade tree selection by farmers impacts agroforests inter‐ and intraspecific trait diversity, and (iii) which shade tree traits should be considered for achieving farmer priorities. We consolidate literature on Coffea arabica (coffee) and Theobroma cacao (cocoa) agroforestry systems to summarize the role of shade tree functional traits in three key ecosystem functions: soil fertility, microclimate modification and crop productivity. We compile global and regional datasets on tree functional trait diversity to show the functional space of agroforestry tree species compared with the overall functional space observed in plants. Despite, or maybe because of, high shade tree diversity, shade tree trait characterization remains coarse and commonly measured at the community scale in the literature. Based on published trait data, we show that farmers adjust the functional composition of shade trees to increase the recycling of soil nutrients (high leaf nitrogen), the production of wood (skewing towards lower wood densities) and the production of fruits (tendency towards high seed size). Common shade trees in coffee and cocoa systems fall in the mid‐range of leaf acquisitive to conservative strategies, providing evidence that expanding shade tree portfolios can improve, or even accelerate, functions. Synthesis and applications: Based on the agroforestry literature and on trait‐environment relationships, we propose a matrix of shade tree traits that influence desirable agroecosystem outcomes for farmers, which can guide fine‐scale coordination of trait expression and agroforestry functions.

An assessment of the shade and ground cover influence on the mitigation of water-driven soil erosion in a coffee agroforestry system

Coffee cultivation under agroforestry systems is one of the main agricultural activities in Central America, but climate change is putting its sustainability at risk. Rainfall erosivity has worsened, thus driving soil losses. Although the vegetal covers in coffee agroforestry systems play a crucial role in controlling erosion, the specific influence of each cover layer remains unclear. In this study, we assessed the influence of the canopy (tree and banana cover and coffee cover) and ground cover (weed and litter) on water driven soil erosion, to determine which type of cover has the most influence on soil erosion control. The study site is situated in the core and buffer zones of the Macizo de Peñas Blancas National Park (Nicaragua), where seventeen coffee sampling plots with an agroforestry system composed of an Inga spp and Musa spp canopy cover were analysed. The results showed that the 19.2 ± 3.4% of the soil surface was affected by erosion and it was mainly related to the litter ground cover (r = –0.95, P < 0.001). Also, this cover presented the best partial correlation (ryl.tcw = –0.93, P < 0.001) when the effects of the other vegetal covers were eliminated. Specifically, the litter cover accounted for 90% of the erosion variability, while the impact of the other types of cover was negligible, accounting for just 1% of the erosion. We conclude that litter layer is more important than canopy cover for effective erosion control, and the main function of shade trees is as a source of litter biomass.

Redesain Penataan Pohon Pada Rest Area Jalibar Desa Oro Oro Ombo, Kota Batu

Title: Redesign of Tree Arrangement in Jalibar Rest Area, Oro Oro Ombo Village, Batu City The Jalibar Rest Area is one of the tourist support facilities in Oro Oro Ombo Village. However, this rest area does not yet have a good green planning plan, even though one of the supporting comfort areas in the rest area is the proper arrangement of trees. This research identifies existing tree species and analyzes their functions, evaluates tree arrangements, and recommends tree arrangements based on their function and planting location. The research was conducted in the Jalibar Rest Area in Oro Oro Ombo Village, Batu District, Batu City, East Java. The analytical methods used are Key Performance Index (KPI), Comparative Study, and Spatial Mapping. Based on field observations, it is known that the Jalibar Rest Area has 123 trees with 16 types of trees. Based on the evaluation results, it is known that the suitability of the tree's function to its location, namely that the outer zone of the rest area should be planted with shade trees, guides and trees of aesthetic value. The parking zone is planted with trees that function as shade trees, guides and absorb pollutants. As well as service zones planted with trees with the functions of shade, absorbing pollutants and aesthetics. Recommendations for redesigning tree arrangements in the Jalibar Rest Area include adjusting the function of trees in each zone by maintaining appropriate tree species, replacing inappropriate tree species, adding and subtracting trees based on their planting distance.

Effects of Coloring Liquid Dipping Time and Surface Finishing Procedures on the Optical Properties of Monolithic Zirconia.

Monolithic zirconia restorations can be produced from self-colored or uncolored blocks. The coloring procedure of uncolored blocks and surface treatments may affect the appearance of the restoration. The purpose of this study is to evaluate the effect of liquid coloring and surface treatments on optical properties of translucent monolithic zirconia. All specimens were cut from zirconia blocks with a thickness of 1 mm. Specimens were dipped in the coloring liquids for 3 min, 5 min, and 7 min and then divided into groups of 10 specimens for surface treatments. Glaze and mechanical polishing were applied, and the color of the specimens was measured under the D65 lighting condition with a spectrophotometer device. Color values obtained from the CIE Lab formula and the translucency parameter (TP), opalescence parameter (OP), and contrast ratio (CR) were calculated. The normality of the data was confirmed with the Shapiro-Wilk test. A three-way analysis of variance (ANOVA) was used to assess the effect of dipping time, liquid shade, and surface treatments. The effect of liquid shade and dipping time on the TP, OP, and CR parameters were statistically significant, and the TP values were reduced with the application of coloring liquid. Mechanical polishing groups had higher OP values and lower TP values than glazed groups. In cases where high translucency is required, it may be advisable to use self-colored blocks and apply glaze as a surface treatment to achieve the desired optical properties.

Effect of planting material and pre-planting storage method on rhizome sprouting, crop stand, and seed rhizome yield of Turmeric (Curcuma longa L.) at Teppi, Southwestern Ethiopia

A two-year study experiment was conducted from January 2021 to December 2022 at the Teppi Agricultural Research Center (TARC) with the aim of identifying suitable planting material and an appropriate pre-planting storage method that can enhance turmeric seed quality and yield under rain-fed conditions. The experiment consisted of three planting materials (mother rhizomes, finger rhizomes, and mixed rhizomes) and five pre-planting storage methods (cemented floor, under tree shade, raised mesh-wired bed, pit with grass cover, and pit with soil cover). These factors were factorially arranged using a randomized complete block design with three replications. The results from the analysis of variance revealed that the seed quality, growth, and seed rhizome yield of turmeric were significantly (p&lt;0.05) influenced by the main effect of both planting material and storage method. However, their interaction effect did not significantly affect these attributes (p&gt;0.05). The highest clean and sprouted seed rhizomes, as well as total sprouts, were recorded in mother seed rhizomes and seed rhizomes stored under tree shade. Conversely, storing mother seed rhizomes on cemented floors resulted in the highest seed weight losses. Finger seed rhizomes stored in soil-covered pits produced the highest percentages of non-sprouted and decayed seed rhizomes. Regarding growth attributes, mother seed rhizomes led to the highest plant stands, plant height, stem girth, leaf growth, and tiller number. Storing seed rhizomes under tree shade performed the best across these growth attributes. Turmeric plants grown from mother seed rhizomes yielded a higher number of mother and finger rhizomes per plant and the highest fresh rhizome yield. Likewise, storing seed rhizomes under tree shade resulted in the highest fresh rhizome yield. In general, utilizing mother seed rhizomes and storing them under tree shade improved turmeric seed quality, growth, and seed rhizome yield. Therefore, farmers in the area are recommended to adopt these practices, but further research is needed to assess their economic feasibility.

Low-emissions and profitable cocoa through moderate-shade agroforestry: Insights from Ghana

Cocoa production is a leading driver of deforestation in the humid-tropics of West Africa. Reconciling climate change mitigation with livelihoods of farmers requires identification of production strategies to concurrently improve yield and profit while curtailing emissions of greenhouse gases (GHG). Using a 2021 plot-survey conducted in Ghana’s Eastern region, we evaluated yields, GHG emissions, and value of production (VOP) (a profit indicator) across a typology representing the diversity of systems at plot-level. The typology was constructed by first stratifying plots according to shade levels and variety (hybrid vs. Amazonia) which resulted in three systems: Hybrid sun, hybrid variety under full-sun (little to no shade); Hybrid shade, hybrid and moderate shade (13–25 shade trees ha−1); and Amazonia, Amazonia under predominantly moderate shade. Next, factor analysis and clustering were used to group plots within each system according to cocoa yield, vegetation, management, and (local) climate conditions. Cluster analysis showed that fertiliser, weeding, pruning, hand pollination, cocoa tree density, and shade tree densities of differing heights were most influential for determination across systems. Hybrid shade had the highest net GHG removal rate at −6.8 ± 1.7 (± 95% CI) Mg CO2eq ha−1 yr−1: 48% and 127% higher (emissions more negative) respectively over Amazonia and Hybrid sun. Hybrid shade additionally had the highest average and least variable VOP among production systems at 669 ± 564 USD ha−1 yr−1, compared to Hybrid sun and Amazonia at 404 ± 442 and 213 ± 280 USD ha−1 yr−1 respectively. These results point to hybrid cocoa grown under moderate shade of 13–25 shade trees ha−1 as optimal for reconciling climate change mitigation with development in West African cocoa.

Penerapan Pertanian Regeneratif pada Perkebunan Kopi

Agronomy not only studies cultivation technology and agricultural production, but also examines the application of agricultural concepts and systems that continue to develop. The rapid and fundamental transformation of the agricultural system called the green revolution is based on the use of high inputs to increase agricultural production. The use of inputs that are not environmentally friendly and the emergence of environmental awareness encourages the transformation of agricultural systems into environmentally friendly agriculture such as ecofarming, organic farming, alternative farming, sustainable agriculture and regenerative agriculture. This article reviews the concepts of sustainable agriculture, degenerative agriculture, regenerative agriculture and the practice of implementing regenerative agriculture on coffee plantations. Degenerative agriculture is an agricultural practice that causes productivity to continue to decline as a result of a decrease in the carrying capacity of the agroecosystem, including soil, plants and other flora, fauna, microbes, climate and society. Sustainable agriculture aims to maintain a desired or improved level of ecosystem function, while regenerative agriculture aims to regenerate, renew, and further improve soil function and ecosystem capabilities in an ever-increasing process. The ecological pillars of regenerative agriculture include (1) soil health, (2) biodiversity, and (3) livestock-crop integration. Regenerative coffee plantation practices include the use of superior clones or varieties, grafting, planting shade trees and mixed crops, and livestock integration.Keywords : Biodiversity, degenerative, integrated, sustainable, regenerative agriculture

Risk analysis and cacao pod survivorship curves to improve yield forecasting methods

Cocoa attainable yields are affected by a complex of pests and diseases in a particular agro-environment. Estimation of yield loss is critical for agronomic and economic decision-making at the farm level. For over 15 months, we monitored 1100 pods from six cocoa clones (PMCT-58, CC-137, CATIE-R1 CATIE-R4, ICS-95, and CCN-51) to assess the incidence of pests and diseases and to elucidate their survival behavior. Cocoa clones grow in a 12-year timber-based cocoa agroforestry plot (1330 cocoa plants ha-1, 100 shade trees ha-1, and 65% shade cover), in La Montaña, Turrialba, Costa Rica. Between 6-12 trees and 120-140 pods per clone of four size classes (very small – 2.5-7 cm, small – 7.1-14 cm, medium – 14.1-21 cm, and large – ≥21 cm) were tagged and measured fortnightly. Pods were selected at two vertical strata along the tree trunk (below and above 1.5 m) and grouped into three generations (rainy, transition, and dry). Competitive risk and survival analysis were performed per clone, generation, pod size class, and pod position along the tree trunk. Overall, pods reached maturity at the age of 155-165 days and were exposed to several hazards that disrupted their development. The Cox model that better described the cumulative risk during pod development included two covariables with substantial explicative power, generation, and clones. Regardless of clone and pod generation, a combination of diseases/pests accounted for about 45% to 65% of yield losses. The Kaplan-Meier survival analysis showed that pod survival was similar between generations but differed among clones within generations. Pods from CATIE-R1, CATIE-R4 and CC-137 clones showed greater survival rates than those of ICS-95, CCN-51 and PMCT-58 clones. The position of the pod along the tree trunk did not influence pod survival rates. We combined pod counting and pod survival rates calculated in this study and contrasted our yield estimations against a widely used prediction method proposed by a certification body. Overall, yield estimated using the UTZ method applied to 90-day-old pods of CATIE-R1 clone consistently overestimated yield predictions in the range of 27-45%. We proposed the combination of pod counting and pod index with a set of clone-specific discount factors; the survival rates, to reliably forecast on-farm cacao yields over time.

Tolerance to mild shading levels in cattail as related to increased photosynthesis and changes in its leaf area and anatomy.

Shading is an environmental factor that has been little investigated regarding its effects on emergent aquatic plants. Typha domingensis Pers. is an emergent macrophyte that demonstrates some plasticity for self-shading, and as it can shade other species in the same area, the effect of shading on its traits deserves further investigation. The objective of the present study was to evaluate the gas exchange, leaf anatomy, and growth of T. domingensis cultivated under increasing shading intensities. The plants were collected and propagated in a greenhouse, and the clones were subjected to four shading intensities: 0% (unshaded), 35%, 73%, and 83% shading created by black nets. Growth traits, clonal production, photosynthesis, transpiration, and leaf anatomy were evaluated. The 73% and 83% shading promoted the death of all plants, but all plants survived in the 35% and unshaded treatments. Compared with the unshaded treatment, the 35% shading treatment promoted a higher photosynthetic rate and greater transpiration, supporting increased growth and production of clones. The increase in the photosynthetic rate in the 35% shading was related to the increase in leaf area which increased the photosynthesis of the whole plant. The 73% and 83% treatments inhibited the development of photosynthetic parenchyma and stomata in T. domingensis, leading to a drastic reduction in photosynthesis and energy depletion. Therefore, T. domingensis does not tolerate intense shading, but its photosynthetic characteristics and growth are favored by mild shading, a factor that may be of great importance for its competitiveness and invasive behavior.

Carbon and Methane as Indicators of Environmental Efficiency of a Silvopastoral System in Eastern Amazon, Brazil

Livestock systems have been identified as major emitters of greenhouse gases due to the use of extensive areas with degraded pastures. The objective of this study was to analyze carbon (CO2) and methane (CH4) fluxes in the atmosphere as indicators of environmental sustainability in silvopastoral systems. CO2 and CH4 fluxes from soil to the atmosphere were monitored in a degraded pasture (predominant species: Panicum maximum cv. Mombaça) grown in full sun and compared with areas with tree species (Bertholletia excelsa, Dipteryx odorata, and Khaya grandifoliola) and productive pasture (Panicum maximum cv. Mombaça) grown in full sun. The study area was in Mojuí dos Campos, western Pará state, Eastern Amazon, Brazil. The evaluations were conducted in a Technological Reference Unit with a silvopastoral system, where animals used the shade of trees during high-temperature periods. The fluxes were measured using an ultraportable greenhouse gas analyzer coupled with static polyvinyl chloride ring chambers installed at the soil–air interface. In conclusion, areas with integrated systems (B. excelsa + pasture and K. grandifoliola + pasture) were better mitigators of CO2 emissions; the highest emissions occurred in the degraded pasture area during the rainiest months. The CH4 fluxes were more intense in the areas with degraded pasture and K. grandifoliola + pasture. Converting degraded pasture areas into integrated crop–livestock–forest systems reduced greenhouse gas emissions in the Amazon over 10 years of implementation. The implementation of integrated crop–livestock–forest systems in long-deforested areas with degraded pastures and a low production capacity showed high potential for changes focused on developing sustainable agriculture in the Amazon.

Determination of feed yield and quality parameters of whole crop durum wheat (Triticum durum Desf.) biomass under agrivoltaic system

AbstractAgrivoltaics represents a key technology for reaching sustainable development goals by reducing the competition between land used for food, for feed, and for electricity. It has been demonstrated that Agrivoltaics can increase land productivity and play a role in the expansion of renewable energy production. This work aimed to study the yield and nutritional characteristics, as well as feeding value for ruminants of Durum wheat biomass grown under agrivoltaic. Two years of controlled experiments revealed that the reduction in light moderately limited wheat yields in the phenological phase of soft dough in standard agrivoltaic trackers (i.e. with a Ground Coverage Ratio (GCR) = 13%), otherwise under extended trackers (i.e. GCR = 41%), the yields was reduced compared to control in whole light. The digestible neutral detergent fiber evaluated after 24 h of in situ rumen incubations increased with shading, resulting in different ratios of acid detergent lignin. More shaded theses also had superior crude and soluble proteins, acid detergent-insoluble protein, acid and neutral detergent fiber than the control. The biomass in the shaded treatments showed a better Ca: P ratio for ruminant nutrition. These characteristics are strategic in forage production, allowing a more flexible harvesting strategy. This additional contribution of the nutritional characteristics of Durum wheat produced on Agrivoltaic Systems could allow a better inclusion of the different types in diets and better management of silage or hay harvesting sites. These results might be helpful in improving biomass production and give valuable information on Durum Wheat under the Agrivoltaic System.

Vehicle-Integrated Photovoltaics—A Case Study for Berlin

Recent developments in vehicle-integrated photovoltaics (VIPV) offer prospects for enhancing electric vehicle range, lowering operating costs, and supporting carbon-neutral transport, particularly in urban settings. This study evaluates the solar energy potential of parking spaces in Berlin, considering challenges like building and tree shading using digital surface models and weather data for solar simulations. Utilizing open datasets and software, the analysis covered 48,827 parking spaces, revealing that VIPV could extend vehicle range by 7 to 14 km per day, equating to a median annual increase of 2527 km. The findings suggest median yearly cost savings of 164 euros from reduced grid charging. However, the environmental benefits of solar vehicle charging were found to be less than those of traditional grid-connected photovoltaic systems. The study introduces a method to pinpoint parking spaces that are most suitable for solar charging.

Growth, Physiological, and Transcriptome Analyses Reveal Mongolian Oak Seedling Responses to Shading

Mongolian oak (Quercus mongolica) is a common building material and landscaping tree species in northern China, with significant economic and ecological value. Its seedling growth is inhibited by high light intensity, but the mechanism by which light stress affects the growth and development of its seedlings remains unclear. In this study, we investigated the phenotypes, physiological processes, and molecular responses of 3-year-old Mongolian oak seedlings under different light treatments: full light (Sck), light shading (S1; 40% light), moderate shading (S2; 20% light), and severe shading (S3; 3% light). Compared to Sck, the S1 and S2 treatments resulted in higher leaf area, photosynthetic pigment content, photosynthesis rates, soluble sugar contents, and soluble protein contents in Mongolian oak seedlings. The S1 and S2 treatments also promoted seedling height and diameter growth and resulted in lower degrees of membrane lipid peroxidation, cell membrane permeability, and antioxidant enzyme activity. In contrast, severe shading (S3) significantly inhibited seedling height and diameter growth due to the lower net photosynthetic rate, and exhibiting higher degrees of membrane lipid peroxidation and cell membrane permeability. Shading treatments (S1 and S2) alleviated the negative effects of strong light on the growth and development of Mongolian oak seedlings, with the S2 treatment having the greatest effect. However, severe shading (S3) inhibited growth and development. A total of 3726 differentially expressed genes (DEGs) were detected in leaves under different shading treatments in RNA sequencing analysis. Among these, 1691, 3150, and 824 DEGs were detected in the Sck-S1, Sck-S2, and S1-S2 comparison groups, respectively. The different shading treatments determined common expression regulation pathways, including carotenoid biosynthesis, photosynthetic antenna proteins, and mitogen activated protein kinase (MAPK) signal transduction. Shading induced increases in gene expression levels in light harvesting complexes, which are related to changes in gene expression in the photosynthetic system, leading to changes in photosynthetic physiology. The expression levels of genes related to reactive oxygen species signal perception and activation enzymes were upregulated in Sck. Together, these findings revealed the response mechanisms of Mongolian oak seedlings to different shading levels at the physiological and molecular levels, providing a scientific basis and technical support for the cultivation and large-scale production of Mongolian oak seedlings.

Effects of Flooding and Shade on Survival, Growth, and Leaf Gas Exchange of Bottomland Tree Species across the Great Lakes Region (USA)

Forested wetlands are common ecosystems within the Great Lakes region (Michigan, Minnesota, and Wisconsin), USA. Projected increases in extreme flooding events and shifting disturbance regimes create challenges for tree regeneration. Forest managers are considering the use of enrichment planting to increase tree species diversity, but limited information is available that quantifies the interactions between the flooding and shade tolerances of candidate tree species. We used a microcosm experiment to manipulate shade and flooding conditions to determine the effects on early survival, growth, and leaf gas exchange of 23 different tree species that vary in shade and flood tolerance. Seedlings were planted in pots and placed in 227 L tanks that were randomly assigned to light reduction (full sun, 40% and 70% reduced sunlight) and flood treatments (water levels of 0, 14, or 27 cm below the soil surface). In general, flooding treatments had a greater influence on seedling growth and leaf gas exchange rates than light reduction treatments. Of the species studied, bald cypress (Taxodium distichum (L.) Rich.) was the most flood-tolerant, but American sycamore (Platanus occidentalis L.) and river birch (Betula nigra L.) were also highly tolerant of flooding conditions throughout the entire growing season. The flood tolerances of the remaining species varied, but none were tolerant of water table depths within 14 cm of the soil surface for the entire growing season. Most species did not respond to the shade treatments in terms of early growth, survival, and leaf gas exchange. When considering species for planting in forested wetlands, matching the flood tolerance of candidate species to local site hydrology is an important step.

LoRa Communication Quality Optimization on Agriculture Based on the PHY Anti-Frame Loss Mechanism

Agricultural environments are usually characterized by height differences and tree shading, which pose challenges for communication in smart agriculture. This study focuses on optimizing the packet loss rate and power consumption of LoRa’s practical communication quality. The research includes the investigation of the PHY anti-frame loss mechanism, encompassing PHY frame loss detection and the response mechanism between gateways and nodes. By implementing a closed loop for transmission and reception, the study enhances the communication network’s resistance to interference and security. Theoretical performance calculations for the SX1278 radio frequency chip were conducted under different parameters to determine the optimal energy efficiency, reducing unnecessary energy waste. An experimental assessment of the packet loss rate was conducted to validate the practical efficacy of the research findings. The results show that the LoRa communication with the anti-frame loss mechanism and the optimal energy ratio parameter exhibits an adequate performance. In the presence of strong and weak interferences, the reception rates are maximally improved by 37.8% and 53.4%, with effective distances of 250 m and 600 m, corresponding to enhancements of 100 m and 400 m, respectively. This research effectively reduces LoRa energy consumption, mitigates packet loss, and extends communication distances, providing insights for wireless transmission in agricultural contexts.

A Kalman Filtering Algorithm for Measurement Interruption Based on Polynomial Interpolation and Taylor Expansion.

Combined SINS/GPS navigation systems have been widely used. However, when the traditional combined SINS/GPS navigation system travels between tall buildings, in the shade of trees, or through tunnels, the GPS encounters frequent signal blocking, which leads to the interruption of GPS signals, and as a result, the combined SINS/GPS-based navigation method degenerates into a pure inertial guidance system, which will lead to the accumulation of navigation errors. In this paper, an adaptive Kalman filtering algorithm based on polynomial fitting and a Taylor expansion is proposed. Through the navigation information output from the inertial guidance system, the polynomial interpolation method is used to construct the velocity equation and position equation of the carrier, and then the Taylor expansion is used to construct the virtual measurement at the moment of the GPS signal interruption, which can make up for the impact of the lack of measurement information on the combined SINS/GPS navigation system when the GPS signal is interrupted. The results of computer simulation experiments and road measurement tests based on the loosely combined SINS/GPS navigation system show that when the carrier faces a GPS signal interruption situation, compared with a combined SINS/GPS navigation algorithm that does not take any rescue measures, our proposed combined SINS/GPS navigation algorithm possesses a higher accuracy in the attitude angle estimation, a higher accuracy in the velocity estimation, and a higher accuracy in the positional localization, and the system possesses higher stability.

Influence of Shade in Cocoa Agroforestry Systems on Physicochemical and Functional Characteristics of Cocoa Beans in Bonon, Central-West Côte d'Ivoire.

Côte d'Ivoire remains the world's leading producer of cocoa beans. However, cocoa farming is now recognized as a primary cause of deforestation in the country. To combat deforestation, the Ivorian government recently advocates for agroforestry, a farming technique involving the cultivation of cocoa trees with fruit or forest trees. Yet, the impact of these associated trees and their shade on the quality of produced cocoa beans remains relatively unknown. This study is aimed at evaluating the influence of tree shade in cocoa farms on the quality of cocoa beans produced in the Bonon area. Morphological, biochemical, and functional analyses were performed on cocoa beans from shaded, partially shaded, and sunny subplots. Overall, only beans from shaded subplots showed better commercial quality. Regarding nutritional potential, results demonstrated that acidity, protein content, and vitamin C levels were influenced by shade. Low protein levels were observed in beans from sunny areas. The presence of moderate shade significantly favored good foaming power and foam stability. These findings play a key role in the perceived quality and application of these beans in the food and cosmetic industry. Moreover, these discoveries open new research perspectives in the field of food biochemistry and sustainable agriculture.

Effect of planting material and pre-planting storage method on rhizome sprouting, crop stand, and seed rhizome yield of turmeric (Curcuma longa L.) at Teppi, Southwestern Ethiopia

A two-year study experiment was conducted from January 2021 to December 2022 at the Teppi Agricultural Research Center (TARC) with the aim of identifying suitable planting material and an appropriate pre-planting storage method that can enhance turmeric seed quality and yield under rain-fed conditions. The experiment consisted of three planting materials (mother rhizomes, finger rhizomes, and mixed rhizomes) and five pre-planting storage methods (cemented floor, under tree shade, raised mesh-wired bed, pit with grass cover, and pit with soil cover). These factors were factorially arranged using a randomized complete block design with three replications. The results from the analysis of variance revealed that the seed quality, growth, and seed rhizome yield of turmeric were significantly (p&lt;0.05) influenced by the main effect of both planting material and storage method. However, their interaction did not significantly affect these attributes (p&gt;0.05). The highest clean and sprouted seed rhizomes, as well as total sprouts, were recorded in mother seed rhizomes and seed rhizomes stored under tree shade. Conversely, storing mother seed rhizomes on cemented floors resulted in the highest seed weight losses. Finger seed rhizomes stored in soil-covered pits produced the highest percentages of non-sprouted and decayed seed rhizomes. Regarding growth attributes, mother seed rhizomes led to the highest plant stands, plant height, stem girth, leaf growth, and tiller number. Storing seed rhizomes under tree shade performed the best across these growth attributes. Turmeric plants grown from mother seed rhizomes yielded a higher number of mother and finger rhizomes per plant and the highest fresh rhizome yield. Likewise, storing seed rhizomes under tree shade resulted in the highest fresh rhizome yield. In general, utilizing mother seed rhizomes and storing them under tree shade improved turmeric seed quality, growth, and seed rhizome yield. Therefore, farmers in the area are recommended to adopt these practices, but further research is needed to assess their economic feasibility.

Transcriptome and metabolome analysis of preharvest internal browning in Nane plum fruit caused by high temperature

The preharvest internal browning of Nane plum fruit, with no visible effects on the appearance of the fruit, has become a serious problem in recent years in its production area in Guangdong Province, China. This study investigated the effects of environmental factors, including temperature, on Nane plum internal browning. Plum orchards at different elevations with different incidences of internal browning were selected. Using fruits with different internal browning incidence levels, the internal browning mechanism was analyzed with transcriptome and metabolome analyses. The results revealed decreased internal browning at high altitudes. Shading treatment significantly reduced internal browning, whereas bagging and insect-proof net-covering treatments significantly increased internal browning. Because bagging and net coverings increase the local ambient temperature, the findings suggest that high temperature is an important factor influencing the internal browning of Nane plum. The metabolome experiments showed that with increased internal browning, the levels of phenolic hydroxyls such as catechol increased, with simultaneous increases in hydrogen peroxide content and oxidase activity. It can be speculated that the oxidation of phenolic hydroxyl substances is the main cause of the preharvest browning of Nane plum. Transcriptome analysis revealed the increased expression of calcium signaling-related and downstream effector genes and indicated an important role of calcium in internal browning, possibly due to its increased content in the fruit. Further, with increasingly serious internal browning, genes related to photosynthesis were down-regulated, while genes related to senescence were up-regulated, thus suggesting the up-regulation of the process of cell senescence during internal browning. In conclusion, heat stress should be eliminated to reduce preharvest internal browning in Nane plum.

Effect of shading on trichome formation and CAPRICE-like gene expression in tea (Camellia sinensis var. sinensis) leaves

Tea (Camellia sinensis var. sinensis) is one of the most popular beverages worldwide and is highly regarded for its unique taste and health benefits. The traditional practice of shading tea plants before harvest is employed to ensure the production of high-quality Japanese green tea. However, the precise effects of shading on trichome formation, leaf tissue structure, and the expression of related genes remain largely unknown. In the present study, we conducted a comparative analysis to investigate the effect of shading on the aforementioned aspects of tea leaves. Our findings revealed a significant decrease in the number of trichomes in the youngest 1st stage leaves under shaded conditions compared with that under full-light conditions. Histological analyses revealed that epidermal cells in tea leaves under shaded conditions were thinner, further indicating the influence of light on leaf structures. To delve deeper into the regulatory mechanisms, the expression levels of six Camellia sinensis CAPRICE (CsCPC) genes encoding R3 MYB transcription factors, which have been implicated in trichome formation in tea plants, were examined using real-time reverse transcription PCR. Among the six genes, CsCPC-1, Camellia sinensis ENHANCER OF TRY AND CPC 1–2 (CsETC1–2), and CsETC3 exhibited increased expression under shaded conditions, indicating their potential involvement in the light-mediated regulation of tea leaf characteristics. The expression levels of the phenylpropanoid pathway structural genes Camellia sinensis cinnamic acid 4-hydroxylase (CsC4H), Camellia sinensis cinnamoyl CoA reductase (CsCCR), and Camellia sinensis flavanone 3′ hydroxylase (CsF3′H), were significantly reduced under shaded conditions, suggesting the regulation of phenylpropanoid content. Our study demonstrated that shading treatment induces significant changes in the number of trichomes in younger leaves, alters the width of epidermal cells, and affects the expression levels of CsCPCs and genes related to the phenylpropanoid pathway. These findings have the potential to enhance tea leaf quality, and offer pragmatic implications for tea farming.