Objectives: Vaginal cytology is a sensitive method for assessing women’s hormonal status, showing variations in menstrual cycle phases. Studies suggest a higher rate of female suicides during the luteal phase. This study aimed to link vaginal cytomorphological profiles with the manner of death and age of the deceased. Materials and Methods: Vaginal smears from 64 autopsy cases were microscopically examined for maturation index (MI) and value, reflecting hormonal profiles. Statistical Analysis: ANOVA between the mean of maturation value with manner of death was found to be significant. Results: Among the 48 suicides, 54.2% showed predominantly intermediate cells in the luteal phase, indicating a mid-zone shift in the MI. The remaining 16 non-suicidal cases showed fewer intermediate cells. Suicides shared a similar cytological profile and MI, especially in the reproductive age group. Conclusions: While vaginal smears are typically used in sexual assault cases to detect sperm, they also offer insights into women’s hormonal profiles. Understanding these profiles could provide clues about psychological status and its relation to the manner of death beyond sexual assault.