Human activities have a complex and multidimensional impact on water quality. The concentration of inhabitants, production and services intensifies influence of urban agglomerations on water in rivers. Among many sources of surface water pollution, the most important are sewage discharges. The aim of the research was to determine the effect of point discharge of treated industrial and municipal wastewater on the distribution of selected water chemical parameters in the Vistula River in Puławy. The studies were carried out in 2018–2019. Samplings were collected in five sampling points and tested in the hydrochemical laboratory. The obtained data were statistically analysed to investigate differences between the sampling points. The negative impact of wastewater discharge on the water quality in the Vistula was found. However, the pollution level decreased with the flow of the river. The parameters tested at measurement point located 1200 m below the discharge approached the values recorded above the sewage outfall. The presented observations of changes in the concentration of pollutants indicate the self-purification capacity of a river. However, for each watercourse flowing through urbanized areas, it is an individual feature. It depends on a number of factors and requires regular monitoring studies taking into account hydrochemical analysis of watercourses.