Sewage Treatment Plant Influent Research Articles

An effective way to utilize solid waste resources: The application of modified fly ash in removing Cu2+ and Pb2+ in wastewater

The sustainable development of society requires the support of developed industrial production, which inevitably produces a series of industrial by-products, including heavy metal-containing wastewater and solid waste. Using solid waste to adsorb heavy metal ions in wastewater is a sustainable way. This not only reduces the environmental threat caused by solid waste accumulation, but also reduces the treatment cost of heavy metal ions in wastewater. In this study, fly ash was modified to prepare magnetic adsorbent (M-AT), which was used to adsorb Cu2+ and Pb2+ in wastewater. The results showed that M-AT has a good adsorption effect on Cu2+ and Pb2+ in wastewater. Its maximum adsorption capacity for Cu2+ and Pb2+ was 53.18 and 85.86 mg/g, respectively. Ion exchange and complexation reaction were major adsorption mechanisms of M-AT for Cu2+ and Pb2+, they played an important role in the adsorption process. M-AT maintained a good adsorption effect on Cu2+ and Pb2+ under different influence factors. This ensured that M-AT can be used in complex application environments. In addition, M-AT exhibited good reusability, its removal rate for Cu2+ and Pb2+ reached 50.21 % and 75.15 % after 5 adsorption–desorption cycles. And the removal rate of M-AT for Cu2+ and Pb2+ in domestic sewage treatment plant influent, tailings drainage, industrial wastewater was 82.80 %, 87.12 %, 85.12 % and 95.10 %, 91.20 %, 93.13 %, respectively. These results proved the feasibility of M-AT in treating Cu2+ and Pb2+ in wastewater. This study provides a new way for the resource utilization of fly ash. Applying solid waste to treat heavy metal-containing wastewater is a sustainable way to treat industrial waste.

Investigating antimicrobial resistance determinants and micropollutants in urban sewage treatment plants of India: Occurrence, removal and ecotoxicological risk

Environmental contamination caused by emerging pollutants (EPs) and their associated ecological risk have received limited attention in India. Thus, this study aims to address this gap by investigating the occurrence and removal of antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs) and pharmaceutical and personal care products (PPCPs) in two sewage treatment plants (STPs) in New Delhi. The study also assessed the ecological risk associated with PPCPs in aquatic environments. Among the targeted ARB and ARGs, erythromycin resistant bacteria and ermB gene, respectively were the most ubiquitous pollutants in the influent of both STPs. In the final effluent, there was a reduction ranging from 0.8 log to 2.8 log units for most ARB, while removal of the majority of ARGs was less than 1 log unit. The secondary treatment demonstrated better removal of total ARG concentrations compared to the tertiary treatment methods employed in both STPs. Regarding PPCPs, metformin was quantified in both influent and effluent samples at a concentration exceeding 19,000 ng/L, indicating its persistence in wastewater. High concentrations of ARB, ARGs, and PPCPs were observed in undigested biosolids, while low to moderate removal of these pollutants was observed after anaerobic digestion. To assess the ecological risk, a risk quotient (RQ) assessment was conducted. The results revealed that compounds such as erythromycin, terbinafine, 17-α ethinylestradiol (EE2), 17-β estradiol (E2), diclofenac, triclosan, carbamazepine, and mefenamic acid exhibited high risks in the effluent. Additionally, EE2 and E2 showed higher risk levels in the digested biosolids, indicating potential risks to the aquatic ecosystem.

Method validation and environmental monitoring of triethanolamine ester quaternary ammonium compounds

In this study water and sediment samples, collected from the River Nene (Northamptonshire) at several sites in the vicinity of the Great Billing sewage treatment plant (STP), were analysed for triethanolamine quaternary compounds (TEAQ, ester quats). A method was developed using liquid chromatography tandem mass spectrometry (LC/MS/MS) with a electrospray ionisation source (ESI). Ten components were determined using a characterised commercial sample of Tallow TEAQ as a standard. To our knowledge this is the first time environmental concentrations of a wide spectrum of individual homologues of TEAQ have been reliably quantified covering a broad range of environmental matrices (STP influent, STP effluent, surface waters and sediments), due to the challenging nature of the analytical method. The method featured novel solutions for the determination of long and multiple chain length alkyl quats, controlling loss processes, background contamination and chromatographic performance. TEAQ compounds were found to be highly removed in the sewage treatment plant resulting in low effluent concentrations. Low concentrations in both river water and sediment samples were found also. In many cases levels were below the Method Detection Limit (MDL). In river water samples, mean values of TEAQ compounds found were 210–398 ng/L for C16:0/C18:0 TEAQ diester and 126–287 ng/L for C18:0/C18:0 TEAQ diester. River sediment was found to contain mean TEAQ levels of 7.07–12.5, 19.7 to 40.3 and 7.04–35.1 μg/kg dry weight for C16:0/C16:0, C16:0/C18:0, and C18:0/C18:0 TEAQ, respectively. At Great Billing STP monoesters and diesters of TEAQ were shown to be efficiently removed (>97 and 99 %, respectively), although limited samples were taken on this occasion.

Distribution of Antibiotic Resistance Genes and Their Association with Microbes in Wastewater Treatment Plants: A Metagenomics Analysis

The extensive use of antibiotics has resulted in the generation and accumulation of antibiotic resistance genes (ARGs) in the environment, and domestic wastewater is one of the main reservoirs of ARGs and resistant bacteria. In this study, Illumina high-throughput sequencing and network analysis were used to study the microbial community characteristics, ARGs’ occurrence status, and resistance mechanism in the influent and effluent of a domestic sewage treatment plant in Changchun, China. A total of 29 phyla were found in the influent and effluent, including 23 bacterial phyla, 3 archaeal phyla, and 3 eukaryotic phyla. In total, 112 ARG subtypes were detected in the samples, and the dominant ARG subtypes were Erm(35) and tet(W/N/W). In this study, ARGs related to tetracycline and macrolide accounted for a high proportion, and the resistance mechanisms of ARGs detected in the samples were mainly antibiotic inactivation and antibiotic efflux pumps. Co-occurrence maps of ARGs and microbes demonstrated by network analysis indicated that the resistance genes kdpE, GES-5, and tetX may easily bind to microbes, potentially making them antibiotic-resistant bacteria. Fifty-seven bacteria in the genera Cupriavidus, Escherichia, and Collinsell are potential hosts of multiple ARGs. The findings can increase our understanding of the distribution of ARGs and their association with microbes in wastewater treatment plants, and also provide a research foundation for controlling the diffusion of ARGs in the environment.

Mass flow and consumption calculations of pharmaceuticals in sewage treatment plant with emphasis on the fate and risk quotient assessment

In Egypt, pharmaceuticals consumption increased dramatically owing to the population growth and the unrestricted sale manner. Accordingly, the occurrence and fate of nine common pharmaceutical active compounds (PhACs) were scrutinized at a sewage treatment plant (STP) in Giza, Egypt. The levels of these PhACs were assessed in different the wastewater treatment stages and dewatered sludge phase using high-performance liquid chromatography coupled with photodiode arrays detector. The average concentrations of the total PhACs detected in influent, primary sedimentation effluent (PSE) and final effluent (FE) were 227, 155 and 89 µg L−1, respectively. The overall removal efficiency of the individual PhACs ranged from 18 to 72% removal. The occurrence trend revealed that biodegradation and adsorption are the concurrently removal mechanisms of the studied PhACs. The overall consumption per day in West of Greater Cairo was estimated based on influent concentration of STP. Sulfamethoxazole, paracetamol and diclofenac were detected with the highest levels in the influent of STP, PSE and FE as well as in the dewatered sludge. Furthermore, the high concentrations of these compounds in the sludge confirm the adsorption pathway removal of theses PhACs. The risk quotient (RQ) assessment for the detected PhACs in FE is greatly higher than the predicted non-effect concentration (PNEC). Conclusively, the FE of STP is considered a risky source for PhACs in adjacent surface water.

Seasonal Variation in Physico-chemical Properties of Effluent Generated at a Sewage Treatment Plant in Varanasi and It’s Suitability for Irrigation

The current investigation was carried out to ascertain the suitability of the influent and effluent of Varanasi's Bhagwanpur sewage treatment plant for irrigation as well as variations in its physicochemical properties throughout the year 2018. The suitability of the water for irrigation was evaluated by taking water samples from the plant's inlet, aerator, and outlet tanks. Then, these samples were examined for heavy metals as well as parameters like pH, EC, TDS, BOD, COD, and dominant anions and cations.The plant's effluent is suitable for irrigating agricultural fields because parameters like pH, nitrate, phosphate, and other cations are below the permissible limit and will provide the necessary nutrients for crops. However, higher concentrations of some heavy metals were a notable concern that will need close attention and ongoing monitoring in the near future if effluent water is to be used for irrigation. The treatment process significantly lowers the effluent water's COD, sulphates, and other ions to levels that allow for surface water discharge, but it leaves the BOD, nitrate, and phosphate content just above levels which are recommended, making it unsuitable for discharge into the holy Ganges since it will cause eutrophication.

Occurrence and Quantitative Microbial Risk Assessment of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Sub-Catchment of the Yodo River Basin, Japan.

The occurrence of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) in a sub-catchment of the Yodo River Basin, a representative water system of a drinking water source in Japan, was investigated. The chromogenic enzyme-substrate medium method was used for the detection of S. aureus and MRSA by the presence or absence of antimicrobials in the medium for viable bacteria in a culture-based setting. The contributions of S. aureus and MRSA from wastewater to the rivers were estimated based on mass flux-based analysis, and quantitative microbial risk assessment (QMRA) was further conducted for S. aureus and MRSA in river environments. The mean abundance of S. aureus and MRSA was 31 and 29 CFU/mL in hospital effluent, 124 and 117 CFU/mL in sewage treatment plant (STP) influent, 16 and 13 CFU/mL in STP effluent, and 8 and 9 CFU/mL in river water, respectively. Contribution of the pollution load derived from the target STP effluent to river water ranged from 2% to 25%. The QMRA showed that to achieve the established health benchmarks, the drinking water treatment process would need to yield 1.7 log10 and 2.9 log10 inactivation in terms of infection risk and disability-adjusted life year (DALY) indexes, respectively. These findings highlight the link between medical environment and the importance of environmental risk management for antimicrobial-resistant bacteria in aquatic environments.

Identification by Liquid Chromatography-Tandem Mass Spectrometry and Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry of the Contributor to the Thyroid Hormone Receptor Agonist Activity in Effluents from Sewage Treatment Plants.

3,3',5-Triiodothyroacetic acid (TRIAC) was identified as a major contributor to the activity of thyroid hormone receptor (TR) agonists in environmental water. TRIAC contributed 60-148% of the TR-agonist activity in effluents from sewage treatment plants (STPs). Meanwhile, the contributions of 3,5,3'-triiodothyronine (T3), 3,3',5,5'-tetraiodothyronine (T4), and analogues were <1%. TRIAC concentrations in the range of 0.30-4.2 ng/L are likely enough to cause disruption of the thyroid system in living aquatic organisms. The origin of TRIAC in the STP effluents was investigated by analyzing both STP influents and effluents. Relatively high concentrations of T3 and T4 (2.5 and 6.3 ng/L, respectively) were found only in the influents. TRIAC was identified only in the effluents. These findings suggested that T3 and T4 in STP influents were potentially converted into TRIAC during activated sludge treatment or by other means. The evaluation of TRIAC at relevant environmental concentrations by in vivo assays and an appropriate treatment to reduce the TR activity in sewage are needed.

Distribution and characteristics of carbapenem-resistant and extended-spectrum β-lactamase (ESBL) producing Escherichia coli in hospital effluents, sewage treatment plants, and river water in an urban area of Japan

Occurrence of profiles of the carbapenem-resistant Escherichia coli (CRE-E) and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-E) in an urban river in a sub-catchment of the Yodo River Basin, one of the representative water systems of Japan was investigated. We conducted seasonal and year-round surveys for the antimicrobial-resistant bacteria (AMRB) and antimicrobial-resistance genes (AMRGs) in hospital effluents, sewage treatment plant (STP) wastewater, and river water; subsequently, contributions to wastewater discharge into the rivers were estimated by analyses based on the mass flux. Furthermore, the characteristics of AMRB in the water samples were evaluated on the basis of antimicrobial susceptibility tests. CRE-E and ESBL-E were detected in all water samples with mean values 11 and 1900 CFU/mL in the hospital effluent, 58 and 4550 CFU/mL in the STP influent, not detected to 1 CFU/mL in the STP effluent, and 1 and 1 CFU/mL in the STP discharge into the river, respectively. Contributions of the pollution load derived from the STP effluent discharged into the river water were 1 to 21%. The resistome profiles for blaIMP, blaTEM, and blaCTX-M genes in each water sample showed that AMRGs were not completely removed in the wastewater treatment process in the STP, and the relative abundances of blaIMP, blaTEM, and blaCTX-M genes were almost similar (P<0.05). Susceptibility testing of antimicrobial-resistant E. coli isolates showed that CRE-E and ESBL-E detected in wastewaters and river water were linked to the prevalence of AMRB in clinical settings. These results suggest the importance of conducting environmental risk management of AMRB and AMRGs in the river environment. To our knowledge, this is the first detailed study that links the medical environment to CRE-E and ESBL-E for evaluating the AMRB and AMRGs in hospital effluents, STP wastewater, and river water at the basin scale on the basis of mass flux as well as the contributions of CRE-E and ESBL-E to wastewater discharge into the river.

Silica removal at sewage treatment plants causes new silica deficiency

The dissolved silicate (DSi) concentration in coastal waters has decreased due to anthropogenic activities. Many studies have indicated that dam construction is a main reason for this reduction. However, recently, dam construction alone has not been sufficient to explain the DSi reduction in some coastal waters. In this study, we focused on silica removal at sewage treatment plants (STPs). DSi and particulate silica (PSi) concentrations were measured in STP influent and effluent waters from September 2020 to September 2021. Dissolution experiments on PSi were also conducted to estimate the fraction of soluble PSi in the STP influent. DSi and PSi were removed by 29.5% and 96.9%, respectively, at the STP. In addition, the soluble PSi in the STP influent accounted for 20.3% of the PSi removed. Therefore, in addition to the DSi removal in STPs, removal of soluble PSi can also cause potential DSi depletion in downstream and coastal waters. In addition to the effect of dams, the silica supply delivered to coastal waters may be further reduced in the future due to the progress of sewage treatment development in coastal areas.

Evaluation of the Correspondence between the Concentration of Antimicrobials Entering Sewage Treatment Plant Influent and the Predicted Concentration of Antimicrobials Using Annual Sales, Shipping, and Prescriptions Data.

The accuracy and correspondence between the measured concentrations from the survey and predicted concentrations on the basis of the three types of statistical antimicrobial use in Japan was evaluated. A monitoring survey of ten representative antimicrobials: ampicillin (APL), cefdinir (CDN), cefpodoxime proxetil (CPXP), ciprofloxacin (CFX), clarithromycin (CTM), doxycycline (DCL), levofloxacin (LFX), minocycline (MCL), tetracycline (TCL), and vancomycin (VMC), in the influent of sewage treatment plant (STP) located in urban areas of Japan, was conducted. Then, the measured values were verified in comparison with the predicted values estimated from the shipping volumes, sales volumes, and prescription volumes based on the National Database of Health Insurance Claims and Specific Health Checkups of Japan (NDB). The results indicate that the correspondence ratios between the predicted concentrations calculated on the basis of shipping and NDB volumes and the measured concentrations (predicted concentration/measured concentration) generally agreed for the detected concentration of antimicrobials in the STP influent. The correspondence ratio on the basis of shipping volume was, for CFX, 0.1; CTM, 2.9; LFX, 0.5; MCL, 1.9; and VMC, 1.7, and on the basis of NDB volume the measured concentration was CFX, 0.1; CTM, 3.7; DCL, 0.4; LFX, 0.7; MCL, 1.9; TCL, 0.6; and VMC, 1.6. To our knowledge, this is the first report to evaluate the accuracy of predicted concentrations based on sales, shipping, NDB statistics and measured concentrations for antimicrobials in the STP influent.

Sewage Markers as Determinants to Differentiate Origins of Emerging Organic Pollutants in an Urban Sri Lankan Water Drainage Network

Urban sanitation is a major challenge during the rapid urbanization being experienced by developing countries, as a low sewerage infrastructure capacity and irregular onsite wastewater treatment raise the risk of surface water contamination. The application of specific sewage markers to characterize contaminant sources is therefore essential for managing urban sanitation issues. In this study, we investigated the concentrations of eight sewage markers (acetaminophen, caffeine, carbamazepine, cotinine, sulfamethoxazole, sulfapyridine, atenolol, and acesulfame) in various water sources within urban area of the Galle City, Sri Lanka. The total concentration of the eight markers was in the order of hospital discharge &gt; sewage treatment plant (STP) influent &gt; surface drainage system. Among the eight selected markers, acetaminophen was dominant in hospital discharge (70.2–123.6 µg/L) while caffeine was the largest contributor to STP influent (16.2–68.7 µg/L) and surface drainage (0.95–21.73 µg/L). We then proposed and tested a set of criteria for evaluating the applicability of markers, including removal efficiency, concentration magnitude, excretion rate, and wastewater burden. The labile markers caffeine and acetaminophen were suitable for characterizing domestic gray and black wastewater, respectively. These results imply that the city’s drainage system receives both domestic graywater and human excretion, likely due to insufficient on-site sanitation systems. The conservative marker carbamazepine was useful for tracking hospital residues over long distances; these results imply that hospital wastewater treatment was not working properly, accounting for pharmaceutical residues reaching surface water via a hidden discharges connected to the drainage system.

Environmental Risk Assessment of Active Human Pharmaceutical Ingredients in Urban Rivers in Japan.

Active pharmaceutical ingredients (APIs) have become a public concern owing to their possible adverse effects on aquatic organisms. Ministry of Health, Labor and Welfare in Japan (MHLW) issued "Guidance on the Environmental Risk Assessment (ERA) in new pharmaceutical development" in 2016. To evaluate the validity of phase 1 in the MHLW's ERA guidance, we monitored the measured environmental concentrations (MECs) of approved APIs in urban rivers and sewage treatment plants (STPs) in Japan and compared these MECs with the predicted environmental concentration (PEC). We collected water samples from urban seven rivers and three STPs during each season. Fifty-one APIs for human and veterinary use and the artificial sweetener sucralose were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Forty-four APIs were observed in the rivers and 42 were found in the influent and effluent of STPs, with levels ranging from nanograms to micrograms per liter. The action limit in phase I of the MHLW's guidance was set to 10 ng/L, and there was no API except for ketoprofen, for which PEC of the MHLW's guidance (PECjapan) was lower than 10 ng/L and the maximum MEC (MECmax) was 10 ng/L or greater. Almost all APIs also had median MECs that were lower than those of the respective PECjapan. These results indicate that the PECjapan values in phase I of the MHLW's guidance were appropriate. However, some APIs had MECmax values that were greater than those of the respective PECjapan due to overestimation of the dilution factor of river water and/or underestimation of API production.

Antibiotic Resistance and Sewage-Associated Marker Genes in Untreated Sewage and a River Characterized During Baseflow and Stormflow.

Since sewage is a hotspot for antibiotic resistance genes (ARGs), the identification of ARGs in environmental waters impacted by sewage, and their correlation to fecal indicators, is necessary to implement management strategies. In this study, sewage treatment plant (STP) influent samples were collected and analyzed using quantitative polymerase chain reaction (qPCR) to investigate the abundance and correlations between sewage-associated markers (i.e., Bacteroides HF183, Lachnospiraceae Lachno3, crAssphage) and ARGs indicating resistance to nine antibiotics (belonging to aminoglycosides, beta-lactams, sulfonamides, macrolides, and tetracyclines). All ARGs, except blaVIM, and sewage-associated marker genes were always detected in untreated sewage, and ermF and sul1 were detected in the greatest abundances. intl1 was also highly abundant in untreated sewage samples. Significant correlations were identified between sewage-associated marker genes, ARGs and the intl1 in untreated sewage (τ = 0.488, p = 0.0125). Of the three sewage-associated marker genes, the BIO-ENV procedure identified that HF183 alone best maximized correlations to ARGs and intl1 (τ = 0.590). Additionally, grab samples were collected from peri-urban and urban sites along the Brisbane River system during base and stormflow conditions, and analyzed for Escherichia coli, ARGs, the intl1, and sewage-associated marker genes using quantitative polymerase chain reaction (qPCR). Significant correlations were identified between E. coli, ARGs, and intl1 (τ = 0.0893, p = 0.0032), as well as with sewage-associated marker genes in water samples from the Brisbane River system (τ = 0.3229, p = 0.0001). Of the sewage-associated marker genes and E. coli, the BIO-ENV procedure identified that crAssphage alone maximized correlations with ARGs and intl1 in river samples (τ = 0.4148). Significant differences in E. coli, ARGs, intl1, and sewage-associated marker genes, and by flow condition (i.e., base vs. storm), and site types (peri-urban vs. urban) combined were identified (R = 0.3668, p = 0.0001), where percent dissimilarities between the multi-factorial groups ranged between 20.8 and 11.2%. Results from this study suggest increased levels of certain ARGs and sewage-associated marker genes in stormflow river water samples compared to base flow conditions. E. coli, HF183 and crAssphage may serve as potential indicators of sewage-derived ARGs under stormflow conditions, and this merits further investigation. Data presented in this study will be valuable to water quality managers to understand the links between sewage pollution and ARGs in urban environments.

Solid-phase optimisation for simultaneous determination of thirteen pharmaceuticals in Ethiopian water samples with HPLC-DAD detection: an initial assessment.

Pharmaceutical consumption is increasing worldwide as it is essential to treat and prevent health issues but they end up in the environment. However, in many African countries like Ethiopia, the status of these compounds in various environmental samples is not very well known. In this study, a simple method for the extraction and determination of thirteen pharmaceutical compounds of different therapeutic classes in water samples using solid-phase extraction and HPLC-DAD was developed. Different parameters affecting extraction were optimised and obtained as hydrophilic-lipophilic balance (HLB) extraction cartridge, water sample pH of 5, elution solvent of 2% formic acid in water with methanol (20:80%, v/v), a sample volume of 150mL and addition of 0.5% w/v EDTA in the sample. The limits of detection and quantification of the optimised method were in the range of 0.1-0.8µg/L and 0.2-2.6µg/L, respectively. The relative recovery in the spiked environmental water sample was in the range of 70-117% except for amoxicillin and acetylsalicylic acid in influent wastewater. The precision for all ranged from 0.3 to 11%. The proposed method was successfully tested for the detection and quantification of different environmental water samples collected from Addis Ababa, Ethiopia. Trimethoprim, caffeine and albendazole concentrations of 7.8 (1.1), 3.2 (0.4) and 2.1 (0.1) µg/L were quantified in hospital wastewater, respectively. The concentration of norfloxacin was found to be below the limit of quantification in the same water. Trimethoprim and ciprofloxacin were also found in the sewage treatment plant influent sample at a concentration of 0.5 (0.02) and 0.3 (0.01) µg/L, respectively.

Disinfection of Antibiotic-resistant Bacteria in Sewage and Hospital Effluent by Ozonation

ABSTRACT The present status of antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) in hospital effluent, sewage treatment plant (STP) influent, STP secondary effluent, STP effluent (chlorination), STP effluent (ozonation), and river water were analyzed in an urban river in Japan by a 2-year monitoring survey. The results revealed the existence of multiple AMRB grouped into six classes whose numbers were in the hospital effluent at 19 to 2,800 CFU/mL, in the STP influent at 9 to 3,133 CFU/mL, in the STP secondary effluent at 1 to 148 CFU/mL, in the STP effluent (chlorination) at N.D. to 191 CFU/mL, and in the river water at N.D. to 28 CFU/mL In the case of the STP effluent (ozonation) the level of AMRB was, however, quite low ranging from N.D. to several CFU/mL, roughly 1/10 to 1/100 of the concentrations detected in the STP effluent (chlorination). The bacterial community structure analysis indicates that only ozonation changed the constitution of bacterial phyla. Rates of antimicrobial resistance were high among MDRA (82% in hospital effluent and 79% in STP influent), MDRP (35% and 40%), and MRSA (93% and 87%). MRSA showed resistance against chlorine treatment but could be gradually disinfected by ozonation. When the hospital effluent and the model STP wastewater prepared by mixing STP influent and STP secondary effluent in a 1:9 ratio were directly treated with ozone, both AMRB and AMSB were inactivated effectively with variance in the time among bacteria, accompanied by a change in the constitution of phyla similar to the observation in the case of the STP effluent (ozonation). Ozonation inactivated almost all target bacteria (>99.9%) after 10 min treatment (CT value: 1.0 mg·min/L). Changing taxonomic diversity of micro-organisms based on 16S rRNA gene sequencing by ozonation showed that ozonation inactivated not only AMRB but also removed antimicrobial resistance genes (AMRGs) in the wastewater. As a result of the present study, a recommendation was evoked to operate ozonation treatment directly for the hospital effluent immediately after flowing out, leading to desirable contribution to reduce environmental pollution, and the potential hazard to human health caused not only by the AMRB and AMSB but also infectious diseases. Extension of the present results opens a new way to assess environmental risks associated with the spread of AMRB and AMSB in aquatic environments to keep the water environment safe and to maintain human health. To our knowledge, this is the first report to show the inactivation profiles of AMRB and AMSB in real STP wastewater together with hospital effluent by direct ozonation.

On-site chlorination responsible for effective disinfection of wastewater from hospital.

Both hospital effluent and a model sewage treatment plant (STP) wastewater prepared by mixing STP influent and STP secondary effluent at a volume ratio of 1:9 were directly treated with chlorine for investigation of their effects on disinfection of antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB). The overall results indicate that the chlorine disinfection effectively inactivated the majority of AMRB and AMSB, expect for MRSA and Staphylococcus aureus in both wastewaters. No significant differences could further be observed in the taxonomic diversity of micro-organisms after the treatment. The degrees of disinfection given by the direct chlorination were comparable to those attained by combination of conventional activated sludge process and additional chlorine treatment at the STP. The results of this study evoked a recommendation to operate local chlorination treatment directly for the wastewater from medicinal facilities prior to its flow into the STP as sewage. Although additional disinfection treatment at the STP seems necessary to remove the recalcitrant MRSA and Staphylococcus aureus, the present study desirably contributes to a great reduction of the loads of STP and urgent prevention of spreading of infectious diseases in the present state.

Occurrence and Sources of Synthetic Musk Fragrances in the Sewage Treatment Plants and the Han River, Korea

Levels of synthetic musk fragrances (SMFs) and various personal care products (PCPs) were measured in the Han River and its tributaries in Seoul, Korea. The most abundant SMF in all river and PCP samples was 4,6,6,7,8,8-hexamethyl-1,3,4,7-tetrahydrocyclopenta(g)sochromene (HHCB), followed by 1-(3,5,5,6,8,8-hexamethyl-6,7-dihydronaphthalen-2-yl)ethanone (AHTN), musk ketone (MK), and 1,1,2,3,3-pentamethyl-2,5,6,7-tetrahydroinden-4-one (DPMI). The most abundant SMF in both PCPs and the Han River samples was HHCB, followed by AHTN. Moving from upstream to downstream in the Han River, the median SMF concentration was 6.756, 2.945, 0.304, and 0.141 μg/L in the sewage treatment plant (STP) influents, effluents, tributaries, and mainstream, respectively, implying that effective SMF removal was achieved during the sewage treatment process, followed by dilution in the receiving water. Four STPs using advanced biological treatment processes had removal efficiencies of 58.5%, 56.8%, and 38.1% for HHCB, AHTN, and MK, respectively. The highest SMF concentrations in the tributaries were observed at locations close to the STPs. Our study confirmed that the main source of SMFs in the receiving water were sewage effluents containing untreated SMFs, which largely originate from household PCPs, especially hair care products (e.g., shampoo) and perfumes.

Analysis of Microbial Communities and Pathogen Detection in Domestic Sewage Using Metagenomic Sequencing

Wastewater contains diverse microbes, and regular microbiological screening at wastewater treatment plants is essential for monitoring the wastewater treatment and protecting environmental health. In this study, a metagenomic approach was used to characterize the microbial communities in the influent and effluent of a conventional domestic sewage treatment plant in the metropolitan city of Jeddah. Bacteria were the prevalent type of microbe in both the influent and effluent, whereas archaea and viruses were each detected at &lt;1% abundance. Greater diversity was observed in effluent bacterial populations compared with influent, despite containing similar major taxa. These taxa consisted primarily of Proteobacteria, followed by Bacteroidetes and Firmicutes. Metagenomic analysis provided broad profiles of 87 pathogenic/opportunistic bacteria belonging to 47 distinct genera in the domestic sewage samples, with most having &lt;1% abundance. The archaea community included 20 methanogenic genera. The virus-associated sequences were classified mainly into the families Myoviridae, Siphoviridae, and Podoviridae. Genes related to resistance to antibiotics and toxic compounds, gram-negative cell wall components, and flagellar motility in prokaryotes identified in metagenomes from both types of samples. This study provides a comprehensive understanding of microbial communities in influent and effluent samples of a conventional domestic sewage treatment plant and suggests that metagenomic analysis is a feasible approach for microbiological monitoring of wastewater treatment.

Effects of natural sunlight on antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) in wastewater and river water

The effects of natural sunlight on antimicrobial-resistant bacteria (AMRB) and antimicrobial-susceptible bacteria (AMSB) were investigated in three types of water: sewage treatment plant (STP) influent, STP secondary effluent, and river water in an urban area of Japan. The AMRB were grouped into six classes: carbapenem-resistant Enterobacteriaceae (CRE), extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E), multi-drug-resistant Acinetobacter (MDRA), multi-drug-resistant Pseudomonas aeruginosa (MDRP), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE). The amount of each group of bacteria present was estimated using specific chromogenic agar formulations. AMRB were detected in all water samples, with 13–2,407 colony-forming units (CFU)/mL in the STP influent, N.D. to 202 CFU/mL in the secondary STP effluent, and N.D. to 207 CFU/mL in the river water. The distribution profiles of the AMSB in water samples were similar to those of AMRB. The degree to which AMRB and AMSB present in the river water were inactivated by natural sunlight was tested as the main aim of this study. Irradiation by natural sunlight was found to inactivate almost 100% of all the target AMRB after 5 h of exposure, with no significant differences (P < 0.05) observed in the effects that sunlight had on AMSB and AMRB. Analysis of the bacterial community structure based on 16S rRNA gene sequencing showed that the structure of the bacterial community was apparently not affected by the exposure to sunlight. In addition, the taxonomic diversity in the STP secondary effluent did not change as a result of additional disinfection with chlorine. The results of this study suggest that it is possible that exposure to sunlight could be used as an alternative to disinfection via chlorine. To our knowledge, this is the first report to demonstrate the mitigation of AMSB and AMRB pollution in a river environment via the exposure to natural sunlight.