Severe Health Research Articles

The Relationship Between Childhood Trauma, Depression and PTSD, Etiology and Treatment Methods

This paper will explore the relationship between childhood trauma and its impact on mental health, feelings of helplessness, and negative cognitive perceptions that lead to these conditions. Moreover, it highlights the important role of parental influence in shaping emotional well-being, with a specific focus on depression and post-traumatic stress disorder (PTSD). Childhood trauma, encompassing experiences such as abuse, neglect, and violence, can significantly elevate the risk of severe mental health issues. Research understates childhood trauma as a predictor of depression and PTSD, both psychosocially and neurobiologically, as trust issues. The paper provides the underlying mechanisms of this relationship, exploring psychosocial perspectives concerning attachment issues, negative beliefs, and emotion dysregulation. Cognitive emotion regulation strategies are emphasized when taking steps for the recovery of childhood trauma and preventing the development of mental disorders; other interventions and treatments, including psychotherapy and medication, offer avenues for healing and recovery. By recognizing the profound impact of childhood trauma on mental health and implementing effective interventions, individuals affected by trauma can embark on a journey toward healing, resilience, and improved well-being.

The Mediterranean diet and periodontitis: A systematic review and meta-analysis

Periodontitis is a severe oral health condition that affects the soft tissue and bone supporting the teeth. The Mediterranean diet has been proposed as a potential contributor to reducing the risk of periodontitis. This systematic review and meta-analysis aims to explore the association between adherence to the Mediterranean diet and periodontitis. A comprehensive literature search from 1992 to January 2024 was conducted across multiple databases, including PubMed, Scopus, Web of Science, and Google Scholar. The included studies were clinical trials, randomized controlled trials, and observational studies that evaluated the impact of the Mediterranean diet on periodontitis. Data extraction and quality assessment of the included studies were performed using standardized protocols. A meta-analysis was conducted to combine effect sizes from multiple studies. This review included seven studies, comprising one cohort study, five cross-sectional studies, and one randomized controlled trial. While some studies reported a potential link between Mediterranean diet adherence and periodontitis, the overall analysis did not demonstrate a significant association. The meta-analysis revealed an Odds Ratio (95 % Confidence Interval) of 0.77 (0.58, 1.03) for the association between adherence to the Mediterranean diet and periodontitis (p = 0.08). This systematic review and meta-analysis found no statistically significant association between periodontitis and Mediterranean diet adherence. Future research should prioritize the implementation of rigorous clinical studies with longer follow-up periods to better understand the causal association between the Mediterranean diet and periodontitis. Observational studies with larger sample sizes are needed to establish more conclusive evidence regarding the impact of dietary patterns on periodontal health.

The Therapeutic Role of Lamotrigine in Borderline Personality Disorder: A Comprehensive Review of Outcomes, Mechanisms, and Treatment Strategies.

Borderline personality disorder (BPD) is a severe mental health condition characterized by pervasive instability in mood, self-image, and interpersonal relationships, significantly impacting individuals' personal, social, and occupational functioning. Current treatment strategies primarily include psychotherapy and pharmacotherapy, but there remains a need for more effective and targeted pharmacological options. This review examines the therapeutic role of lamotrigine in BPD, focusing on its efficacy, safety, mechanisms of action,and practical treatment strategies. A comprehensive review of the existing literature was conducted, including clinical trials, observational studies, and relevant pharmacological data. Key focus areas included lamotrigine's impact on BPD symptoms, pharmacological profile, and comparative effectiveness with other treatments. Lamotrigine has shown promise in managing BPD symptoms, particularly in stabilizing mood and reducing emotional dysregulation and impulsivity. Clinical trials suggest that lamotrigine can effectively address core symptoms of BPD, with a safety profile generally comparable to other treatments. The medication's mechanism of action, which involves modulation of glutamate release and mood stabilization, aligns with the therapeutic goals for BPD. Lamotrigine represents a potential adjunctive treatment for BPD, offering benefits in mood stabilization and symptom management. Integrating psychotherapy and other pharmacological options should be considered within a multimodal treatment approach. Further research is needed to better understand its long-term efficacy and safety and its role in combination therapy.

Study on the influence of active oxygen on the natural oxidation of arsenopyrite under different temperature conditions

Arsenic (As), a toxic element, contaminates farmlands, rivers, and groundwater, posing severe environmental and health risks. Notably, As-containing materials in tailings are affected by temperature variations during long-term storage, and this considerably impact the oxidation and migration of elements in arsenopyrite.This study focused on arsenopyrite and investigated the process of its oxidative dissolution and release of arsenic under different temperature conditions by using in-situ XRD, in-situ XPS and electron paramagnetic resonance spectroscopy(EPR), The role of oxygen free radicals in the oxidation of arsenopyrite was elucidated. It has been established that under high-temperature conditions As, iron (Fe), and sulfur (S) are primarily present As(V)/As(IV), Fe(III), and SO42−, respectively. The O2⋅− generated during the oxidation of As(III) by O2, OH⋅ produced by the Fe(II)/FeOH2+ reaction, and H2O2 formed via their interaction play a crucial role in the photochemical oxidation of arsenopyrite. These findings provide a theoretical basis for the formation of ferric arsenate precipitation, contributing in the adsorption and immobilisation of oxidatively released arsenic.

Stress Coping Framework As an Effort To Improve Quality of Life Patients with Chronic Obstructive Pulmonary Disease: A Literature Review

ABSTRACT COPD patients usually experience a decrease in quality of life due to COPD symptoms such as coughing, shortness of breath,exacerbationAcute disease and comorbidities that occur in COPD patients cause limitations in daily activities. Stress coping is one of the factors that causes good or bad self-care behavior in COPD patients. To describe the influence, stress coping and level of selfcare in COPD patients on their quality of life. The method of this study is a literature review. Literature searches using electronic databases such as PubMed, Google Scholar and Science Direct. A literature review was carried out on international journals published in the last 5 years, available in full text. The keywords used were "self-care", stress coping" and "quality of life" for COPD patients. The selection of articles followed the PRISMA approach. This review summarizes eighteen articles. Five articles discussed self-care, the SelfEfficacy scale, and discriminative validity with higher self-care scale scores in individuals with greater COPD severity and poorer health status. Five articles discuss coping stress in COPD sufferers. COPD patients who have good knowledge will have more coping mechanism strategies so that their quality of life becomes more prosperous. Seven articles discussed the quality of life of COPD sufferers. If a COPD patient has good stress coping and strong confidence and confidence in his or her abilities, this will influence self-care actions and improve the COPD patient's quality of life. Future researchers are encouraged to explore similar research themes using other journal search engines to enrich the study. Keywords: Self Care, Stress Coping, Quality Of Life, COPD

Long-Term Effectiveness and Outcomes of Bariatric Surgery: A Comprehensive Review of Current Evidence and Emerging Trends.

Obesity is a global epidemic associated with an increased risk of severe health conditions such as type 2 diabetes, cardiovascular diseases, and certain cancers. Bariatric surgery has become a pivotal treatment for severe obesity, offering significant improvements in weight loss and comorbidity resolution. This comprehensive review aims to assess the long-term effectiveness and outcomes of various bariatric surgical procedures, highlighting current evidence and emerging trends in the field. We extensively reviewed the literature, including randomized controlled trials, cohort studies, and meta-analyses, to evaluate long-term weight loss, resolution of obesity-related comorbidities, quality of life (QoL), and complications associated with different bariatric procedures. Bariatric surgery has demonstrated substantial and sustained weight loss over the long term, with varying degrees of effectiveness among different procedures. Gastric bypass and sleeve gastrectomy are associated with significant improvements in comorbidities such as type 2 diabetes and hypertension. QoL outcomes are generally positive, improving physical health, mental well-being, and social functioning. However, long-term complications, including nutritional deficiencies and the need for reoperations, remain challenges. Emerging trends such as minimally invasive techniques and nonsurgical interventions show promise in enhancing patient outcomes. Bariatric surgery remains a highly effective intervention for managing severe obesity and its related health issues. While long-term outcomes are generally favorable, continued advancements in surgical techniques and postoperative care are crucial for optimizing results and minimizing complications. Future research should focus on personalized approaches to patient management and the development of novel treatment modalities to further improve outcomes in the long term.

The Flavivirus Non-Structural Protein 5 (NS5): Structure, Functions, and Targeting for Development of Vaccines and Therapeutics.

Flaviviruses, including dengue (DENV), Zika (ZIKV), West Nile (WNV), Japanese encephalitis (JEV), yellow fever (YFV), and tick-borne encephalitis (TBEV) viruses, pose a significant global emerging threat. With their potential to cause widespread outbreaks and severe health complications, the development of effective vaccines and antiviral therapeutics is imperative. The flaviviral non-structural protein 5 (NS5) is a highly conserved and multifunctional protein that is crucial for viral replication, and the NS5 protein of many flaviviruses has been shown to be a potent inhibitor of interferon (IFN) signalling. In this review, we discuss the functions of NS5, diverse NS5-mediated strategies adopted by flaviviruses to evade the host antiviral response, and how NS5 can be a target for the development of vaccines and antiviral therapeutics.

Side effects and perceptions among young adults in Bangladesh following COVID-19 vaccination: a single center study

Background and objectives: COVID-19, caused by SARS-CoV-2, has led to a global pandemic with severe health, economic, and social impacts. Vaccination has emerged as a crucial mitigation strategy. Despite the pivotal role of COVID-19 vaccines in controlling the pandemic, vaccine hesitancy remains a significant concern globally, particularly among young adults. This study aimed to explore the side effects and perceptions of the young adults in Bangladesh following COVID-19 vaccination. Materials and methods: The study, conducted in April 2021 among 325 young Bangladeshi adults who received two doses of Sinopharm (BBIBP-CorV) vaccine against SAR-CoV-2. Participants completed a self-administered online questionnaire covering demographics, health history, post-vaccination adverse events, and perceptions about COVID-19 vaccine. A symptom scoring system, based on the interquartile range (IQR), was used to categorize the severity of the side effects. Data analysis utilized SPSS version 26.0, with appropriate tests for significance. Result: Total 325 participants (male - 64.6%, female - 68.9%) were enrolled. The mean age was 22 ± 1.6 years. Social media (43·1%) was the primary source of information about COVID-19. Vaccine related side effects were experienced by 40.9% and 47.1% participants following 1st and 2nd dose of vaccination respectively. Side effects were more prevalent after the second dose of vaccine, particularly in females (31·3% vs. 8·2%, p<0·001). Common side effects included fatigue (41·6%), injection site pain/swelling (36·7%) and headache (32·6%). In over 50% of participants, symptoms appeared within 8 hours following both doses. Symptoms resolved by taking rest at home in majority of participants. Participants with comorbidity reported significantly higher rate of side effects after the first dose (61.8% vs. 37·3%, p <0.05). Despite side effects, 69·8% felt reassured post-vaccination, 63·7% believed in its long-term safety, and 98·8% recommended vaccination to others. Conclusion: The Sinopharm COVID-19 vaccine was well-tolerated among young adults in Bangladesh. Though higher side effects after the second dose were observed in female participants, yet most maintained a positive perception, underscoring its acceptability and recommended vaccination to others. July 2024; Vol. 18(2):011. DOI:https://doi.org/10.55010/imcjms.18.023 *Correspondence: Md. Faizul Ahasan, Department of Pharmacology, Ibrahim Medical College, 1/A Ibrahim Sarani, Segunbagicha, Dhaka 1000, Bangladesh. Email: arronnoo_shuvro@live.com

From not knowing, to knowing more needs to be done: health care providers describe the education they need to care for sex trafficked patients

BackgroundSex trafficking is highly prevalent, pernicious, and under-recognized. When an individual is trafficked for the purpose of sexual exploitation within the borders of a single country, it is termed domestic sex trafficking. Sex trafficked persons can experience severe physical and mental health outcomes requiring medical attention and treatment. However, health care providers often fail to identify sex trafficked patients, missing opportunities to provide needed care and support.MethodsIn this qualitative study, we interviewed 31 health care providers (physicians, nurses, and social workers) working in Ontario, Canada to learn what they identified as their specific education and training needs to recognize and care for sex trafficked persons. Interviews were conducted over Zoom, recorded, and transcribed. Coding of the transcripts followed a standard framework for qualitative studies. Codes related to the education and training needs of providers were identified as a core issue suited to further analysis.ResultsThree themes related to providers’ education and training needs emerged. These acknowledge basic (Foundational knowledge), as well as more specific learning needs (Navigating the encounter). The final theme, (“It just seems so much bigger than me”) suggests that even with some knowledge of domestic sex trafficking, participants still experienced considerable distress and multiple challenges due to gaps in the broader system impacting the provision of appropriate care.ConclusionsParticipants voiced their need for specialized sex trafficking education as well as role specific training to combat their sense of inadequacy and provide better care for their patients. Participants’ education needs ranged from requiring the definition of domestic sex trafficking and the frequency of its occurrence, to the various circumstances associated with increased risk of recruitment into sex trafficking. In terms of desired training and specific skills, participants wanted to learn how to identify a person being sex trafficked, broach the subject with a patient, know what to do next including access to local resources and referrals, as well as connections to other critical services, such as legal and housing. The results can be used to inform the design and content of education and training on sex trafficking for health care providers.

Magnitude and determinants of road traffic accidents in North Gondar Zone; Amhara Region, Ethiopia

Background Road traffic accidents are a severe health concern in Ethiopia. This study aims to examine the primary contributing variables of road traffic accidents in Ethiopia, North Gondar Zone, under 24 administrative wereda’s. Methods This study utilized secondary data collected from both the werda-level and North Gondar central-level offices within the study areas. The sector police typically submit reports to the zonal-level offices annually. Information regarding crashes, fatalities, and injuries during the study period was extracted from these police reports. The research focused on analyzing the frequency and severity of traffic accidents attributed to various factors of drivers, vehicles, road conditions, pedestrians, and environmental influences. Results In a sample of 603 aggregate road traffic accidents, the data reveals that the majority of damage results are attributed to young drivers, accounting for 58% of the cases, with junior grade level drivers contributing 29.4%. Driving without a license was a significant factor, representing 87%, while accidents involving recruited driver stood at 63.2%. Vehicles with small to articulated trucks contributing to 39%. Mechanical failures were prevalent, with an unknown diagnosis rate of 48.3%. Vehicles in service for 2-5 years accounted for 29.4%. Road surface conditions were 46%, while rural roads saw a higher percentage at 51.2%. Weather conditions played a role also influential, as dry asphalt and gravel roads under main access ownership were linked to of accident, with 91.2% occurring during visible daylight hours between 8:00-12:00 and 15:00-17:00. The victims were predominantly farmers at 49.6%, followed by students at 23%. Conclusions To reduce road traffic accidents in the study area, it is essential to manage causative factors such as driver and road users, vehicles, roads, pedestrians, and environmental behaviors. The study suggests the development of a successful, up-to-date traffic accident database system regionally within respective offices.

Childhood obesity is associated with a high degree of metabolic disturbance in children from Brazilian semi-arid region

Projected to impact 310 million children by the next decade, childhood obesity is linked to serious health issues like metabolic disturbance and cardiovascular diseases. This study introduces a novel approach for the integrated assessment of inflammatory, glycemic and lipid disorders in obese children in resources-limited settings and also identifies key factors contributing to these changes. Conducting a cross-sectional analysis of 231 children aged 5–12 years from public schools in Brazil's semi-arid region, the research involved collecting medical history, anthropometric measurements, and blood samples to analyze glycemic and lipid profiles, along with C-reactive protein levels. We used an adapted the Molecular Degree of Perturbation model to analyze deviations in metabolic markers from a healthy control group. Statistical analyses included Mann–Whitney and Fisher exact tests, backward logistic regression, and hierarchical cluster analysis. The study identified a direct and independent association between elevated Metabolic Disturbance Degree and both overweight and obesity in children, with significant differences in CRP, Triglycerides, and HDL levels noted between obese and healthy-weight groups. The findings highlight the critical need for early detection and comprehensive understanding of obesity-related changes to mitigate the severe health risks associated with childhood obesity.

Prenatal cigarette smoke exposure sensitizes acetaminophen-induced liver injury by modulating miR-34a-5p in male offspring mice.

Introduction: Cigarette smoke (CS) exacerbates the severity of diseases not only in lungs, but also in systemic organs having no direct contact with smoke. In addition, smoking during pregnancy can have severe health consequences for both the mother and the fetus. Therefore, our aim was to evaluate effects of prenatal exposure to CS on acetaminophen (APAP)-induced acute liver injury (ALI) in offspring. Methods: Female C57BL/6 mice on day 6 of gestation were exposed to mainstream CS (MSCS) at 0, 150, 300, or 600μg/L for 2h a day, 5days a week for 2weeks using a nose-only exposure system. At four weeks old, male offspring mice were injected intraperitoneally with a single dose of APAP at 300mg/kg body weight to induce ALI. Results: Maternal MSCS exposure significantly amplified pathological effects associated with ALI as evidenced by elevated serum alanine aminotransferase levels, increased hepatocellular apoptosis, higher oxidative stress, and increased inflammation. Interestingly, maternal MSCS exposure reduced microRNA (miR)-34a-5p expression in livers of offspring. Moreover, treatment with a miR-34a-5p mimic significantly mitigated the severity of APAP-induced hepatotoxicity. Overexpression of miR-34a-5p completely abrogated adverse effects of maternal MSCS exposure in offspring with ALI. Mechanistically, miR-34a-5p significantly decreased expression levels of hepatocyte nuclear factor 4 alpha, leading to down-regulated expression of cytochrome P450 (CYP)1A2 and CYP3A11. Discussion: Prenatal exposure to MSCS can alter the expression of miRNAs, even in the absence of additional MSCS exposure, potentially increasing susceptibility to APAP exposure in male offspring mice.

In Vivo Antischistosomicidal and Immunomodulatory Effects of Dietary Supplementation with Taraxacum officinale.

Bilharziasis is a widespread trematode parasite that poses a severe public health burden. Dandelion (Taraxacum officinale) has several pharmacological and traditional properties critical for treating several hepatic disorders. The present study was designed to assess the potential efficacy of T. officinale root (TOR) dietary supplementation with or without praziquantel (PZQ) against liver and intestinal disorders in mice infected with Schistosoma mansoni. This study was conducted on five groups; G1: uninfected control, G2: untreated S. mansoni-infected mice, G3: infected animals treated with 250 mg/kg PZQ for three alternative days, G4: infected animals were orally administered 600 mg/kg bw TOR daily for 10 days, and G5: infected animals that received both PZQ and TOR as previously described. The current findings after different treatments indicated topographical scanning electron microscopy alterations of male adult worms and a critical reduction in worm burden, ova count, granuloma diameter, hepatic and intestinal histological abnormalities, fibrosis, immunohistochemical expression of CD3+ and CD20+ cells, oxidative stress, and interleukin-10, also upregulation of interferon-gamma, and antioxidant enzymes, when compared to the infected untreated mice. The best results were obtained in mice administered PZQ+TOR together because of their antioxidant properties and ability to promote the host immune response to parasitic infection.

Identification of steroid-induced osteonecrosis of the femoral head biomarkers based on immunization and animal experiments

BackgroundSteroid-induced osteonecrosis of femoral head (SONFH) is a severe health risk, and this study aims to identify immune-related biomarkers and pathways associated with the disease through bioinformatics analysis and animal experiments.MethodUsing SONFH-related datasets obtained from the GEO database, we performed differential expression analysis and weighted gene co-expression network analysis (WGCNA) to extract SONFH-related genes. A protein-protein interaction (PPI) network was then constructed, and core sub-network genes were identified. Immune cell infiltration and clustering analysis of SONFH samples were performed to assess differences in immune cell populations. WGCNA analysis was used to identify module genes associated with immune cells, and hub genes were identified using machine learning. Internal and external validation along with animal experiments were conducted to confirm the differential expression of hub genes and infiltration of immune cells in SONFH.ResultsDifferential expression analysis revealed 502 DEGs. WGCNA analysis identified a blue module closely related to SONFH, containing 1928 module genes. Intersection analysis between DEGs and blue module genes resulted in 453 intersecting genes. The PPI network and MCODE module identified 15 key targets enriched in various signaling pathways. Analysis of immune cell infiltration showed statistically significant differences in CD8 + t cells, monocytes, macrophages M2 and neutrophils between SONFH and control samples. Unsupervised clustering classified SONFH samples into two clusters (C1 and C2), which also exhibited significant differences in immune cell infiltration. The hub genes (ICAM1, NR3C1, and IKBKB) were further identified using WGCNA and machine learning analysis. Based on these hub genes, a clinical prediction model was constructed and validated internally and externally. Animal experiments confirmed the upregulation of hub genes in SONFH, with an associated increase in immune cell infiltration.ConclusionThis study identified ICAM1, NR3C1, and IKBKB as potential immune-related biomarkers involved in immune cell infiltration of CD8 + t cells, monocytes, macrophages M2, neutrophils and other immune cells in the pathogenesis of SONFH. These biomarkers act through modulation of the chemokine signaling pathway, Toll-like receptor signaling pathway, and other pathways. These findings provide valuable insights into the disease mechanism of SONFH and may aid in future drug development efforts.

Antimicrobial Resistance in Equines: A Growing Threat to Horse Health and Beyond-A Comprehensive Review.

The equine industry holds substantial economic importance not only in the USA but worldwide. The occurrence of various infectious bacterial diseases in horses can lead to severe health issues, economic losses, and restrictions on horse movement and trade. Effective management and control of these diseases are therefore crucial for the growth and sustainability of the equine industry. While antibiotics constitute the primary treatment strategy for any bacterial infections in horses, developing resistance to clinically important antibiotics poses significant challenges to equine health and welfare. The adverse effects of antimicrobial overuse and the escalating threat of resistance underscore the critical importance of antimicrobial stewardship within the equine industry. There is limited information on the epidemiology of antimicrobial-resistant bacterial infections in horses. In this comprehensive review, we focus on the history and types of antimicrobials used in horses and provide recommendations for combating drug-resistant bacterial infections in horses. This review also highlights the epidemiology of antimicrobial resistance (AMR) in horses, emphasizing the public health significance and transmission dynamics between horses and other animals within a One Health framework. By fostering responsible practices and innovative control measures, we can better help the equine industry combat the pressing threat of AMR and thus safeguard equine as well as public health.

The Prevention of Viral Infections: The Role of Intestinal Microbiota and Nutritional Factors.

Viral infections pose significant global challenges due to their rapid transmissibility. Therefore, preventing and treating these infections promptly is crucial to curbing their spread. This review focuses on the vital link between nutrition and viral infections, underscoring how dietary factors influence immune system modulation. Malnutrition, characterized by deficiencies in essential nutrients such as vitamins A, C, D, E, and zinc, can impair the immune system, thereby increasing vulnerability to viral infections and potentially leading to more severe health outcomes that complicate recovery. Additionally, emerging evidence highlights the role of commensal microbiota in immune regulation, which can affect hosts' susceptibility to infections. Specific dietary components, including bioactive compounds, vitamins, and probiotics, can beneficially modify gut microbiota, thus enhancing immune response and offering protection against viral infections. This review aims to elucidate the mechanisms by which dietary adjustments and gut microbiota impact the pathogenesis of viral infections, with a particular focus on strengthening the immune system.

When Needs Don’t Translate into Wants: Choosing the Right Tone to Communicate About Healthy Behaviors

ABSTRACT Although individuals should benefit from adhering to communication that promotes healthier behaviors, such communication often encounters mixed success. To understand this incongruity, we identify a misconception about the roles of two distinct drivers of health behavior: needs (which are more objective) and wants (which are more subjective). While needs generally translate into wants in ordinary consumption contexts, they sometimes clash with wants in the context of health behaviors. We propose that communicators’ failure to anticipate this misalignment leads them to choose an inadequate communication tone (more or less assertive). Across three studies conducted in the field and online, adopting the perspectives of both communicators and receivers, we examine how the misalignment of needs and wants in health contexts affects the selection of communication tone and reactions to this communication. We find that communicators (and study participants taking the role of communicators) frequently select the tone (more or less assertive) of their communication based on the receivers’ (objective) needs. Still, conversely, receivers’ responses depend on how the tone of communication matches their (subjective) wants. This gap tends to disappear for more severe health issues, where wants align better with needs. The frequent misalignment of needs and wants in the health domain challenges health communication effectiveness. Communicators should seek ways to address this misalignment, for example, by increasing the perceived severity of the health issue or the perceived authority of the source.

Tracking Childhood Lead Exposure in Early Industrial Romanians

Childhood lead exposure has been linked to severe adverse health outcomes throughout life. Measurements of lead in teeth have established that individuals living in contaminated environments show higher levels compared to individuals living further away, although less is known about when individuals are most susceptible to these exposures. We examined lead (Pb208) concentrations (ppm) in teeth over the first 2.5 years of life in 16 children born in the late 19th to early 20th century throughout Romania. This period of intense industrialization was characterized by increases in mining, coal burning, and oil refining—activities that contaminate air, water, and food with Pb. We hypothesized the distance from an operational mine or oil refinery, or being born in a city, would be positively associated with cumulative dentine Pb exposure (CDPE). We also predicted that Pb exposures would peak in the first six months of life when gastrointestinal (GI) absorption of Pb is likely highest. Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) of sectioned tooth dentine followed by Bayesian statistical analyses revealed that living 30 km or more from a mine or oil refinery did not explain CDPE. However, being born in a city explained 42% of CDPE. All individuals showed maximum Pb exposures after six months of age, likely due to contaminated solid food and/or non-milk liquids. This research demonstrates how tooth formation can be coupled with comprehensive elemental mapping to analyse the context and timing of early-life neurotoxicant exposures, which may be extended to well-preserved teeth from clinical and historic populations.

Isolation of aflatoxin biosynthetic inhibitor from Chondrostereum purpureum mushroom culture filtrate

Aflatoxins (AFs) are highly toxic mycotoxins produced by the fungi, Aspergillus flavus and Aspergillus parasiticus. AFs pose severe health risks owing to their acute toxicity and carcinogenic properties. The control of AF contamination remains significantly challenging despite the extensive efforts toward controlling it. Here, we investigated the potential of mushroom extracts as a source of AF biosynthetic inhibitors. The A.parasiticus mutant strain, NFRI-95, that accumulates an AF biosynthesis intermediate, norsolorinic acid, was used in the bioassay to detect the inhibitory activity against AF biosynthesis. The screening of 195 mushroom extracts revealed that the culture filtrate extract of Chondrostereum purpureum exhibited strong inhibitory activity against AF biosynthesis. Next, large-scale culturing of C.purpureum was performed to isolate the compounds accounting for the inhibitory activity. The culture filtrate was extracted with ethyl acetate, after which the active compound was isolated by silica gel column chromatography and preparative high performance liquid chromatography (HPLC). The active compound was identified as cyclo(Val-Pro) by spectroscopic analyses. Further, four stereoisomers of cyclo(Val-Pro) were synthesized by the condensation of the N-Boc derivatives of d- and l-valine with the methyl esters of d- and l-proline. The naturally isolated compound was identified as cyclo(l-Val-l-Pro) by comparing its retention time with those of synthetic compounds by chiral HPLC analysis and CD spectra. The IC50 value of cyclo(L-Val-L-Pro) was 2.4mM, whereas the LD, DL, and DD isomers exhibited weaker activities, with IC50 values of >5mM.

Early vs. Later Experiences of Violence and Polysubstance Use Among Adults Who Inject Drugs

Background People who inject drugs (PWID) experience high rates of violence, especially in early childhood, increasing their likelihood of engaging in risky substance use behavior in adulthood. Additionally, complex trauma has been reported among PWID due to witnessing and experiencing an overdose, further highlighting the need to examine the role of multiple experiences of trauma on their vulnerability to substance misuse. Methods Our study of 350 PWID from rural Kentucky examined differences in polysubstance use between participants who experienced violence earlier (≤15 years old) versus later (≥16 years old) in their childhood. Results Findings highlighted a direct association between experiences of early childhood violence and polysubstance use in adulthood. Additionally, our sample of PWID who experienced violence early in their childhood reported higher rates of severe substance use disorder and mental health distress. Conclusions Tailored approaches that incorporate trauma-informed care may help to address the disproportionate rates of substance use and related adverse effects among PWID.