Complex Set Research Articles

AUTOMATIC SYSTEM FOR ADVANCED SPEED CONTROL OF THE DRILLING HOE

Drilling consists of the operation of creating a well, which involves a complex set of activities. In a narrower sense, it includes the dislocation of rocks and the extraction of rock fragments to the surface. A drilling tool called a bit is used to dislodge rocks, being connected to the surface drilling rig via a drill string. Automating the processes associated to drilling is extremely important to ensure its development in optimal conditions. This work is focused on the control of the drilling hoe rotation speed, in order to damage it as little as possible.

Responding to financing uncertainty in complex settings: the case of immigrant entrepreneurs from the Arab world in Sweden

ABSTRACT This paper investigates the financing decisions of immigrant entrepreneurs (IEs) in complex settings in host countries, where uncertainties surrounding access to finance persist. While previous studies have acknowledged the presence of financing barriers, they have not sufficiently explained how IEs manage to sustain their entrepreneurial activities. To address this gap, this study presents a dynamic analysis of the financing decision-making process of IEs based on qualitative data gathered from 30 interviews with IEs from the Arab world residing and operating in Sweden. Findings reveal a three-phase decision-making process influenced by situational and dispositional factors. Additionally, the study captures distinct patterns of financing choices made by IEs when confronting uncertainty in complex settings. Specifically, it captures a behavioural aspect of being financially ‘ambidextrous’ or ‘non-ambidextrous’ across multiple contexts.

CAR-T Therapy in Treating Solid Tumor: Application and Limitations

CAR-T therapy has emerged as a revolutionary approach in the battle against cancer. By leveraging the body's own immune system, this technique harnesses the power of T cells, which are genetically engineered to recognize specific antigens found on cancer cells. The procedure involves isolating lymphocytes from patients, followed by laboratory-based genetic modifications to infuse them with distinct CAR genes. Once these enhanced CAR-T cells are reintroduced into the patient, they actively target and neutralize cancer cells. However, the journey of CAR-T therapy is not without challenges. The selection of the right antigen is paramount, as an incorrect choice can lead to unintended consequences. Moreover, the application of this therapy in solid tumors presents its own set of complexities. This review aims to shed light on the clinical potential of CAR-T therapy, the obstacles faced in the realm of solid tumor treatment, and the ongoing research endeavors to refine and improve this promising therapeutic strategy.

Lighting up Lipidic Nanoflares with Self-Powered and Multivalent 3D DNA Rolling Motors for High-Efficiency MicroRNA Sensing in Serum and Living Cells.

Intelligent DNA nanomachines are powerful and versatile molecular tools for bioimaging and biodiagnostic applications; however, they are generally constrained by complicated synthetic processes and poor reaction efficiencies. In this study, we developed a simple and efficient molecular machine by coupling a self-powered rolling motor with a lipidic nanoflare (termed RMNF), enabling high-contrast, robust, and rapid probing of cancer-associated microRNA (miRNA) in serum and living cells. The lipidic nanoflare is a cholesterol-based lipidic micelle decorated with hairpin-shaped tracks that can be facilely synthesized by stirring in buffered solution, whereas the 3D rolling motor (3D RM) is a rigidified tetrahedral DNA scaffold equipped with four single-stranded "legs" each silenced by a locking strand. Once exposed to the target miRNA, the 3D RM can be activated, followed by self-powered precession based on catalyzed hairpin assembly (CHA) and lighting up of the lipidic nanoflare. Notably, the multivalent 3D RM that moves using four DNA legs, which allows the motor to continuously and acceleratedly interreact with DNA tracks rather than dissociate from the surface of the nanoflare, yielded a limit of detection (LOD) of 500 fM at 37 °C within 1.5 h. Through the nick-hidden and rigidified structure design, RMNF exhibits high biostability and a low false-positive signal under complex physiological settings. The final application of RMNF for miRNA detection in clinical samples and living cells demonstrates its considerable potential for biomedical imaging and clinical diagnosis.

Pre-collisional architecture of the European distal margin: Inferences from the high-pressure continental units of central Corsica (France)

Abstract The Lower Units of Alpine Corsica, France, are fragments of continental crust strongly deformed and metamorphosed under high-pressure metamorphic conditions. Three slices of Lower Units are well exposed in the area between the Asco and Tavignano valleys, Central Corsica. Despite their complex structural setting, they provide the opportunity for a reconstruction of the pristine stratigraphic setting of the Lower Units. In our reconstruction, these units consist of a Paleozoic basement topped by Triassic to Early Jurassic sedimentary rocks unconformably covered by Middle to Late Eocene foredeep deposits. However, the three units exposed in the study area display strong differences mainly in the thickness of the Mesozoic sequence. These differences are here interpreted as acquired during the first stage of the rifting process in a setting controlled by normal faults. During the collision-related tectonics and the accretion of the Lower Units to the Alpine orogenic wedge, these normal faults were probably reactivated with a reverse kinematics. The stratigraphic logs of the Lower Units strictly resemble those of the Pre-Piedmont Units from Western Alps. This similarity indicates a common origin of the Lower Units and the Pre-Piedmont Units from the same domain (i.e., the European distal continental margin).

Lethal perturbation of an Escherichia coli regulatory network is triggered by a restriction-modification system's regulator and can be mitigated by excision of the cryptic prophage Rac.

Bacterial gene regulatory networks orchestrate responses to environmental challenges. Horizontal gene transfer can bring in genes with regulatory potential, such as new transcription factors (TFs), and this can disrupt existing networks. Serious regulatory perturbations may even result in cell death. Here, we show the impact on Escherichia coli of importing a promiscuous TF that has adventitious transcriptional effects within the cryptic Rac prophage. A cascade of regulatory network perturbations occurred on a global level. The TF, a C regulatory protein, normally controls a Type II restriction-modification system, but in E. coli K-12 interferes with expression of the RacR repressor gene, resulting in de-repression of the normally-silent Rac ydaT gene. YdaT is a prophage-encoded TF with pleiotropic effects on E. coli physiology. In turn, YdaT alters expression of a variety of bacterial regulons normally controlled by the RcsA TF, resulting in deficient lipopolysaccharide biosynthesis and cell division. At the same time, insufficient RacR repressor results in Rac DNA excision, halting Rac gene expression due to loss of the replication-defective Rac prophage. Overall, Rac induction appears to counteract the lethal toxicity of YdaT. We show here that E. coli rewires its regulatory network, so as to minimize the adverse regulatory effects of the imported C TF. This complex set of interactions may reflect the ability of bacteria to protect themselves by having robust mechanisms to maintain their regulatory networks, and/or suggest that regulatory C proteins from mobile operons are under selection to manipulate their host's regulatory networks for their own benefit.

Machine Learning Prediction of the Experimental Transition Temperature of Fe(II) Spin-Crossover Complexes.

Spin-crossover (SCO) complexes are materials that exhibit changes in the spin state in response to external stimuli, with potential applications in molecular electronics. It is challenging to know a priori how to design ligands to achieve the delicate balance of entropic and enthalpic contributions needed to tailor a transition temperature close to room temperature. We leverage the SCO complexes from the previously curated SCO-95 data set [Vennelakanti et al. J. Chem. Phys. 159, 024120 (2023)] to train three machine learning (ML) models for transition temperature (T1/2) prediction using graph-based revised autocorrelations as features. We perform feature selection using random forest-ranked recursive feature addition (RF-RFA) to identify the features essential to model transferability. Of the ML models considered, the full feature set RF and recursive feature addition RF models perform best, achieving moderate correlation to experimental T1/2 values. We then compare ML T1/2 predictions to those from three previously identified best-performing density functional approximations (DFAs) which accurately predict SCO behavior across SCO-95, finding that the ML models predict T1/2 more accurately than the best-performing DFAs. In addition, we study ML model predictions for a set of 18 SCO complexes for which only estimated T1/2 values are available. Upon excluding outliers from this set, the RF-RFA RF model shows a strong correlation to estimated T1/2 values with a Pearson's r of 0.82. In contrast, DFA-predicted T1/2 values have large errors and show no correlation to estimated T1/2 values over the same set of complexes. Overall, our study demonstrates slightly superior performance of ML models in comparison with some of the best-performing DFAs, and we expect ML models to improve further as larger data sets of SCO complexes are curated and become available for model training.

THE PLACE OF LEGAL NORMS ON APPEALING AGAINST ACTIONS (INACTION) AND DECISIONS IN THE CRIMINAL PROCEDURE LAW OF RUSSIA: PROBLEM STATEMENT

The article examines the criminal procedural norms governing criminal procedural appeal in the aspect of the main scientific directions on the topic under study. The purpose of the study is to determine the essence and the place of the legal norms of appeal in the criminal procedure law, the specifics of legal relations regulated by them. Materials and methods. To achieve the purpose of the study, the method of materialistic dialectics, logical, systemic and structural methods were used. The empirical basis of the study was made up of court decisions and the results of research performed by other authors. Study results. The nature of the emerging links between the legal norms of appeal in the Russian criminal procedure legislation is analyzed, the features of legal relations regulated by them are investigated, their place in the sectoral subject of criminal procedure regulation is determined. The main scientific directions on the subject under consideration, in conjunction with the author's conclusions, made it possible to identify a relatively independent area of the law on the topic under study. Conclusions. The author comes to the conclusion on heterogeneity of the legal norms of appeal, on different conditions for realizing the right to a complaint, depending on the stage of criminal proceedings and the subject of law. In connection therewith, a different legal regulation of legal relations in connection with the criminal procedure appeal is inevitable. The results of the study give the opportunity to conclude that the issues of appeal are not limited in their legal regulation by one universal legal institution of the industry and represent a more complex set of norms having relative independence within the branch of criminal procedure law.

Performance and Analysis of the Alchemical Transfer Method for Binding-Free-Energy Predictions of Diverse Ligands.

The Alchemical Transfer Method (ATM) is herein validated against the relative binding-free energies (RBFEs) of a diverse set of protein-ligand complexes. We employed a streamlined setup workflow, a bespoke force field, and AToM-OpenMM software to compute the RBFEs of the benchmark set prepared by Schindler and collaborators at Merck KGaA. This benchmark set includes examples of standard small R-group ligand modifications as well as more challenging scenarios, such as large R-group changes, scaffold hopping, formal charge changes, and charge-shifting transformations. The novel coordinate perturbation scheme and a dual-topology approach of ATM address some of the challenges of single-topology alchemical RBFE methods. Specifically, ATM eliminates the need for splitting electrostatic and Lennard-Jones interactions, atom mapping, defining ligand regions, and postcorrections for charge-changing perturbations. Thus, ATM is simpler and more broadly applicable than conventional alchemical methods, especially for scaffold-hopping and charge-changing transformations. Here, we performed well over 500 RBFE calculations for eight protein targets and found that ATM achieves accuracy comparable to that of existing state-of-the-art methods, albeit with larger statistical fluctuations. We discuss insights into the specific strengths and weaknesses of the ATM method that will inform future deployments. This study confirms that ATM can be applied as a production tool for RBFE predictions across a wide range of perturbation types within a unified, open-source framework.

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Virtual reality relaxation for mental health staff in complex care services: A feasibility and acceptability study

ObjectiveVirtual reality (VR) relaxation has been implemented in workplaces for staff wellbeing. However, little is known about potential benefits for mental health staff who work in challenging settings who can experience high stress levels and poor wellbeing. MethodsThis study evaluated implementation of a single-session VR relaxation intervention for staff in complex care mental health settings. Pre- and post-VR visual analogue scales (VAS) explored the effect of VR on wellbeing. Post-VR, VAS on helpfulness and immersiveness were recorded, participants planned a behavioural activity to explore the real-world application of VR relaxation, and narrative feedback was collected. ResultsParticipants (N = 22) were mostly of Black ethnicity, in their early forties, and just over half were female. There was an even mix of outpatient and inpatient staff from various professions. Most were nurses, occupational therapists, or doctors. Post-VR, for the total sample, there were significant increases in relaxation, happiness, connectedness to nature (all d>1.00 and p<0.00); and decreases in stress, anxiety (both d>1.00 and p<0.00) and sadness (d = 0.53, p = 0.02). There was no significant effect of sadness for outpatient staff, whereas inpatient staff experienced a significant decrease post-VR (d = 68, p = 0.03). Mean helpfulness and immersiveness ratings were high. All participants planned a behavioural activity, and 15 participants (68 %) carried it out. Participants found the VR intervention to be relaxing, soothing, exciting and an immersive respite from work. ConclusionThe positive findings indicate potential for wider implementation of VR-based wellbeing interventions in mental health settings and other workplaces where staff experience high stress levels.

Chinese Journalists’ Tactics for Reporting on Sexual Violence Against Women

ABSTRACT In 2018, Chinese journalists found themselves up against a complex set of challenges when engaging with reporting on sexual violence against women in the news. The Chinese media sphere promotes “positive energy” (zheng nengliang正能量) to maintain social harmony and what can be considered “positive” content. Since sexual violence incidents are categorized as a “negative” and “sensitive” social issue, they are often the target of complex censorship and editorial revisions. Through semi-structured in-depth interviews with 22 Chinese journalists, this study has identified four tactics Chinese journalists use when reporting on sexual violence against women in order to avoid this censorship: (1) citing official sources as a form of implicit permission or authorization; (2) stressing the educational value of sexual violence stories; (3) using social media and public opinion to indirectly disseminate the reporting of sexual violence; and (4) publishing stories before official directives are issued. This study thus aims to shed light on the challenges faced by Chinese journalists in terms of reporting on sexual violence as a result of the dual pressures from both state and market forces, as well as how they navigate these challenges.

Perceived trauma nursing core competency, interprofessional collaborative competency, and associated barriers among regional trauma center nurses

IntroductionAs key members of interprofessional teams working in complex settings, nurses in trauma centers require trauma nursing core competency. PurposeThis study sought to determine the levels and relationships of the perceived importance and performance of trauma nursing core competency, as well as the interprofessional collaborative competency and associated barriers among Korean regional trauma center nurses. MethodsThis cross-sectional, descriptive, and correlational survey involved a convenience sample of 190 Korean trauma center nurses. Data were collected using a web-based self-reporting questionnaire about the perceived importance and performance of trauma nursing core competency, as well as the interprofessional collaborative competency and associated barriers. Data were analyzed using descriptive statistics, Pearson’s correlation, and multiple regression (Enter method) analyses. ResultsThe perceived performance and importance of interprofessional collaborative competency, the perceived importance of trauma nursing core competency, and the perceived barriers to resources, training, competency, and interest significantly affected trauma nursing core competency performance, accounting for 64.5 % of the variance. ConclusionsTraining programs are needed to improve the core and interprofessional collaborative competencies of trauma nurses. Individual, team, and organizational approaches are essential to addressing the perceived barriers. The effects of training programs on the core competency of trauma nurses should be validated.

Exogenous abscisic acid and sugar induce a cascade of ripening events associated with anthocyanin accumulation in cultured Pinot Noir grape berries.

Fruit quality is dependent on various factors including flavour, texture and colour. These factors are determined by the ripening process, either climacteric or non-climacteric. In grape berry, which is non-climacteric, the process is signalled by a complex set of hormone changes. Abscisic acid (ABA) is one of the key hormones involved in ripening, while sugar availability also plays a significant role in certain ripening aspects such as anthocyanin production. To understand the relative influence of hormone and sugar signalling in situ can prove problematic due to the physiological and environmental (abiotic and biotic) factors at play in vineyards. Here we report on the use of in vitro detached berry culture to investigate the comparative significance of ABA and sugar in the regulation of Pinot noir berry anthocyanin production under controlled conditions. Using a factorial experimental design, pre-véraison berries were cultured on media with various concentrations of sucrose and ABA. After 15 days of in vitro culture, the berries were analysed for changes in metabolites, hormones and gene expression. Results illustrated a stimulatory effect of sucrose and ABA on enhancing berry colour and a corresponding increase in anthocyanins. Increased ABA concentration was able to boost anthocyanin production in berries when sucrose supply was low. The sucrose and ABA effects on berry anthocyanins were primarily manifested through the up-regulation of transcription factors and other genes in the phenylpropanoid pathway, while in other parts of the pathway a down-regulation of key proanthocyanindin transcription factors and genes corresponded to sharp reduction in berry proanthocyanidins, irrespective of sucrose supply. Similarly, increased ABA was correlated with a significant reduction in berry malic acid and associated regulatory genes. These findings suggest a predominance of berry ABA over berry sugar in coordinating the physiological and genetic regulation of anthocyanins and proanthocyanins in Pinot noir grape berries.

Exogenous melatonin orchestrates multiple defense responses against Botrytis cinerea in tomato leaves

Infection of tomato leaves and fruits by necrotrophic fungal pathogen Botrytis cinerea causes considerable economic loss. Melatonin has multiple physiological functions and is considered a potential biocontrol agent for its positive effects on plant innate immunity. In tomato, melatonin reduces Botrytis-caused post-harvest fruit decay by activating jasmonic acid (JA) signaling. In this study, we analyzed the effects of melatonin on resistance to Botrytis and associated defense mechanisms in tomato leaves. Treatment of detached tomato leaves with melatonin at a concentration as low as 100 µM induced resistance to Botrytis based on reduced lesion size and fungal growth. Melatonin-induced resistance to gray mold was associated with activation of a complex set of defense mechanisms in tomato leaves. First, melatonin suppressed Botrytis-induced cell death and accumulation of reactive oxygen species (ROS), which was correlated with increased activities of ROS-scavenging enzymes and enhanced expression of their corresponding genes. Second, melatonin treatment induced callose synthase genes and promoted rapid and strong callose deposition in tomato leaves. Melatonin also induced other anti-microbial genes encoding chitinases, β-1,3-glucanases, phenylalanine ammonialyases and polyphenol oxidases. Third, melatonin increased Botrytis-induced expression of genes encoding SlWRKY33, SlMYC2 and SlERF transcription factors, which activate defense-related genes expression through JA and ethylene (ET) signaling pathways. By contrast, melatonin suppressed salicylic acid (SA) signaling based on reduced expression of the SlPR1 marker gene, which is known to antagonize defense against Botrytis. These results collectively indicate that exogenous melatonin orchestrates multiple signaling pathways to activate a complex set of defense mechanisms against Botrytis in tomato leaves.

REHABS: An Innovative and User-Friendly Device for Rehabilitation.

Rehabilitation is a complex set of interventions involving the assessment, management, and treatment of injuries. It aims to support and facilitate an individual's recovery process by restoring a physiological function, e.g., limb movement, compromised by physical impairments, injuries or diseases to a condition as close to normal as possible. Innovative devices and solutions make the rehabilitation process of patients easier during their daily activities. Devices support physicians and physiotherapists in monitoring and measuring patients' physical improvements during rehabilitation. In this context, we report the design and implementation of a low-cost rehabilitation system, which is a programmable device designed to support tele-rehabilitation of the upper limbs. The proposed system includes a mechanism to acquire and analyze data and signals related to rehabilitation processes.

Genetics, genomics and mechanisms responsible for high levels of pyrethroid resistance in Musca domestica

Insecticide resistance is both economically important and evolutionarily interesting phenomenon. Identification of the mutations responsible for resistance allows for highly sensitive resistance monitoring and allows tools to study the forces (population genetics, fitness costs, etc.) that shape the evolution of resistance. Genes coding for insecticide targets have many well-characterized mutations, but the mutations responsible for enhanced detoxification have proven difficult to identify.We employed multiple strategies to identify the mutations responsible for the extraordinarily high permethrin resistance in the KS17-R strain of house fly (Musca domestica): insecticide synergist assays, linkage analysis, bulk segregant analyses (BSA), transcriptomics and long read DNA (Nanopore) sequencing. The >85,100-fold resistance in KS17-R was partially suppressed by the insecticide synergists piperonyl butoxide and S,S,S-tributylphosphorothionate, but not by diethyl maleate nor by injection. This suggests the involvement of target site insensitivity, CYP-mediated resistance, possibly hydrolase mediated resistance and potentially other unknown factors. Linkage analysis identified chromosomes 1, 2, 3 and 5 as having a role in resistance. BSA mapped resistance loci on chromosomes 3 and 5. The locus on chromosome 3 was centered on the voltage sensitive sodium channel. The locus on chromosome 5 was associated with a duplication of multiple detoxification genes. Transcriptomic analyses and long read DNA sequencing revealed overexpressed CYPs and esterases and identified a complex set of structural variants at the chromosome 5 locus.

The Impact of Physical Exercises on the Psychomotor Capacities of Middle School Pupils

The research aims to identify the adolescents’ avoidance causes from physical education lessons and to determine the means of improving students' satisfaction with physical education. The present research is of ascertaining – ameliorative type, carried out on a sample of 15 teachers and 180 pupils from secondary classes in the Secondary School "Alexandru Ioan Cuza" and the Secondary School no 10 Bacau, Romania, between March 1, 2022 and June 10, 2022. The methods used in the research included: the analysis of the specialized literature, the diagnosis questionnaire, and the mathematical-statistical method. The main conclusions refer to the identification of the passionate fields and the preferences for the sport of the pupils, the argumentation of the need to introduce in the physical education and sports classes competitive sports games, which, besides the training of some motor qualities, ensure an extremely complex set of psychic sentimental, emotional, volitional and cognitive nature acquisitions.

Cultural Adaptation and Integration of the Vietnamese Community in Australia

The Vietnamese community in Australia has faced numerous challenges during its integration into Australian society. Starting with language barriers and cultural differences, the community has had to navigate a complex set of social, economic, and political factors to establish its position in the country. Despite these obstacles, they have maintained their cultural identity while embracing the Australian way of life. One of the most significant contributions of the Vietnamese community to Australian society has been their ability to bring about fresh perspectives and opportunities for cultural exchange. The younger generations of Vietnamese Australians, in particular, have been instrumental in highlighting the values of inclusivity and multiculturalism. They have been at the forefront of promoting cultural diversity and understanding and acceptance of different communities. Our research aims to systematically document the adaptation and integration of the Vietnamese community in Australia across different periods. We employ qualitative methods to analyse the various factors that have contributed to the success of the Vietnamese community in Australia. By doing so, we hope to provide a comprehensive understanding of the challenges and opportunities faced by the Vietnamese community during their integration into Australian society.

Neurofilaments in health and Charcot-Marie-Tooth disease.

Neurofilaments (NFs) are the most abundant component of mature neurons, that interconnect with actin and microtubules to form the cytoskeleton. Specifically expressed in the nervous system, NFs present the particularity within the Intermediate Filament family of being formed by four subunits, the neurofilament light (NF-L), medium (NF-M), heavy (NF-H) proteins and α-internexin or peripherin. Here, we review the current knowledge on NF proteins and neurofilaments, from their domain structures and their model of assembly to the dynamics of their transport and degradation along the axon. The formation of the filament and its behaviour are regulated by various determinants, including post-transcriptional (miRNA and RBP proteins) and post-translational (phosphorylation and ubiquitination) modifiers. Altogether, the complex set of modifications enable the neuron to establish a stable but elastic NF array constituting the structural scaffold of the axon, while permitting the local expression of NF proteins and providing the dynamics necessary to fulfil local demands and respond to stimuli and injury. Thus, in addition to their roles in mechano-resistance, radial axonal outgrowth and nerve conduction, NFs control microtubule dynamics, organelle distribution and neurotransmission at the synapse. We discuss how the studies of neurodegenerative diseases with NF aggregation shed light on the biology of NFs. In particular, the NEFL and NEFH genes are mutated in Charcot-Marie-Tooth (CMT) disease, the most common inherited neurological disorder of the peripheral nervous system. The clinical features of the CMT forms (axonal CMT2E, CMT2CC; demyelinating CMT1F; intermediate I-CMT) with symptoms affecting the central nervous system (CNS) will allow us to further investigate the physiological roles of NFs in the brain. Thus, NF-CMT mouse models exhibit various degrees of sensory-motor deficits associated with CNS symptoms. Cellular systems brought findings regarding the dominant effect of NF-L mutants on NF aggregation and transport, although these have been recently challenged. Neurofilament detection without NF-L in recessive CMT is puzzling, calling for a re-examination of the current model in which NF-L is indispensable for NF assembly. Overall, we discuss how the fundamental and translational fields are feeding each-other to increase but also challenge our knowledge of NF biology, and to develop therapeutic avenues for CMT and neurodegenerative diseases with NF aggregation.