Setback Viscosity Research Articles

Ultrasound-Assisted Extraction of Mango (Mangifera indica) Kernel Starch: Chemical, Techno-Functional, and Pasting Properties

(1) Background: Starch is the main component of mango (Mangifera indica) kernel, making it an alternative to obtain an ingredient from a non-conventional source with potential application in food and other industrial applications; however, reports on the use of new extraction techniques for this material are scarce. The main objective of this research was to evaluate the effect of ultrasound-assisted extraction (UAE) on the yield, chemical, techno-functional, rheological, and pasting properties of starch isolated from a non-conventional source such as a mango kernel. (2) Methods: Different power sonication conditions (120, 300, and 480 W) and sonication time (10, 20, and 30 min) were evaluated along with a control treatment (extracted by the wet milling method). (3) Results: Ultrasound-assisted extraction increases starch yield, with the highest values (54%) at 480 W and 20 min. A significant increase in the amylose content, water-holding capacity, oil-holding capacity, solubility, and swelling power of ultrasonically extracted starches was observed. Similarly, mango kernel starch (MKS) exhibited interesting antioxidant properties. The sol-gel transition temperature and pasting parameters, such as the breakdown viscosity (BD) and the setback viscosity (SB), decreased with ultrasound application; (4) Conclusion: indicating that ultrasound caused changes in physical, chemical, techno-functional, rheological, and pasting properties, depending on the power and time of sonication, so it can be used as an alternative starch extraction and modification technique, for example, for potential application in thermally processed food products such as baked goods, canned foods, and frozen foods.

Effects of dry heating, acetylation, and acid pre-treatments on modification of potato starch with octenyl succinic anhydride (OSA)

Abstract Octenyl succinic anhydride (OSA) starch is widely used to stabilize emulsions. Nevertheless, the poor compatibility of starch with hydrophobic groups has restricted the performance of OSA modification. In this work, potato starch was pre-treated once or twice (dry heating, acetylation, and acid modification) prior to OSA modification. Pre-treatments increased the degree of substitution (DS), hydrophobicity, hydrophilicity, and decreased amylose content of OSA starches, with dual pre-treatments having greater effects. Among all pre-treatments, acid modification followed by dry heating resulted in the greatest OSA modification (DS: 0.015) and water-binding capacity (155%). Meanwhile, acid modification followed by acetylation produced OSA starch with the highest oil-binding capacity (290%). Scanning electron microscopy revealed that the granular deformation of dual pre-treated OSA starches was greater compared to single pre-treated and non-pre-treated OSA starches (O). Dual pre-treated OSA starches (ADO, 7%; ACO, 8%) had lower amylose contents than those of single pre-treated (AO: 12%, CO: 17%, DO: 21%) and O (36%). All the pre-treatments reduced the setback viscosity of OSA starch to a lower range (70–394 cP), simultaneously decreasing their retrograde tendency. This study suggested that dual pre-treatments could improve the efficiency of OSA modification and produce OSA starch with greater emulsifying potential.

Optimization of slow-release fertilizer application improves lotus rhizome quality by affecting the physicochemical properties of starch

To achieve the dual goals of high yield and good quality with low environmental costs, slow-release fertilizer (SRF) has been widely used in lotus cultivation as new type of fertilizer instead of traditional nitrogen fertilizer. However, the optimal amount of SRF and how it would promote lotus rhizome quality remain unclear. This study was designed to investigate the photosynthetic characteristics and the synthesis, accumulation, and physicochemical properties of lotus rhizome starches under six SRF levels (CK, S1, S2, S3, S4, and S5). Compared with CK (0 kg ha–1), the net photosynthetic rate (Pn) and SPAD values of leaves remained at higher levels under SRF treatment. Further research showed that SRF increased the lotus rhizome yield, the contents of amylose, amylopectin, and total starch, and the number of starch granules. Among the six SRF levels, S3 (1 035 kg ha–1) showed the greatest difference from CK and produced the highest levels. With the increasing SRF levels, the peak, hot and final viscosities decreased at first and then increased, but the setback viscosity and pasting temperature increased. In order to interpret these changes at the molecular level, the activities of key enzymes and relative expression levels of starch accumulation related genes were analyzed. Each of these parameters also increased under SRF treatment, especially under the S3 treatment. The results of this study show that SRF, especially S3 (1 035 kg ha–1), is a suitable fertilizer option for lotus planting which can improve lotus rhizome quality by affecting starch accumulations related enzymes and genes. These results will be useful for SRF application to high-quality lotus rhizome production with low environmental costs.

Physicochemical and functional properties of the protein-starch interaction in Chinese yam.

Protein-starch interaction has an important impact on the properties of starchy foods rich in protein, but the contribution of the interaction to Chinese yam still remains unclear. This study aimed to characterize the physicochemical and functional properties related to the possible interaction between starch and protein in Chinese yam. Differential scanning calorimetry and rapid viscosity analyzer results revealed that the gelatinization temperature increased in protein and starch cross-linked powder, while the peak viscosity and the setback viscosity decreased. The swelling power and solubility at 80°C and 95°C decreased with increasing protein ratio in the powder. In vitro starch digestibility test indicated that a high protein ratio could rapidly reduce digestible starch, but increase both slowly digestible starch and resistant starch. Protein could act as the physical barrier toward starch against heating and digestion to exert the influence on starch properties. Fourier transform infrared spectroscopy test revealed the interaction between protein and starch. These results revealed the role of protein-starch interaction and provided beneficial information for the utilization of Chinese yam.

Effects of Bran and Hull Retention on the Sensory and Nutrients Composition of “Ogi Flour” Prepared from Maize, Millet and Sorghum

The effect of Bran and Hull retention on the sensory and nutrient composition of “Ogi flour prepared from maize, millet are sorghum were investigated. The maize and sorghum were soaked for 72 hours, while the millet was soaked for 48 hours, decanted, washed and wet milled into a paste which was oven dried at 50°C for 24 hours and milled into fine flour. The proximate composition, functional properties, pasting properties of the “Ogi” flour was determined as well as evaluating the sensory properties. The moisture content, ash, fat, crude fibre, protein and carbohydrate respectively ranged thus: 2.63 – 8.67%, 1.13 – 1.41%, 4.57 – 7.20%, 1.30 – 5.75%, 4.27 – 7.87% and 75.84 – 82.88%. The presence of Bran and Hulls led to a decrease in moisture content, on increase in ash fat and protein content with decrease in carbohydrate values. The bulk density, water absorption capacity, swelling power and solubility respectively ranged as follows: 0.56 – 0.61g/ml, 1.00 – 2.00g/g, 5.50 – 6.95g/g, 16.10 – 24.40% the presence of Bran led to a decrease in bulk density in maize and millet ogi, a decrease in water absorption capacity in millet and sorghum ogi. The pastry properties of peak, trough breakdown, final and setback viscosities showed a decrease in values from the control samples values for peak time died not differ significantly (P<0.05) between the sieved and unseived ogi flours. While the presence of Bran and Hulls increased pasting temperature in maize and sorghum ogi flours. Result for sensory evaluation showed the control samples were the most preferred for all the attributes. This study revealed that ogi flour can be prepared with a by-pass of the sieving process to enhance nutrient retention.

Comparison of the physicochemical properties of starches from maize reciprocal F1 hybrids and their parental lines

Heterosis on maize yield and quality is highly variable and depends on parental selection. This study investigated and compared the starch structure and physicochemical properties among four sweet-waxy maize lines, four waxy maize lines, and their eight reciprocal F1 hybrids. Compared with the sweet-waxy maize, waxy maize and F1 hybrids had lower extent of branching of amylopectin and relative crystallinity, and larger starch granule size. Waxy maize starch had higher breakdown viscosity and retrogradation percentage, and lower setback viscosity and gelatinization enthalpy than the sweet-waxy maize starch. Meanwhile, the peak and setback viscosities, and retrogradation enthalpy of most F1 hybrid starches were higher than those of their female parent, while gelatinization enthalpy was the opposite. The F1 hybrid starches had higher onset temperature and retrogradation percentage and lower gelatinization enthalpy than their male parent in general. In conclusion, this study provides a framework for the production of new hybrids.

Gelatinization Properties of Parboiled Siramog Varieties Black Rice with Variation of Immersion Time and Sodium Citrate Concentration

Black rice still contains epidermis which is composed of several layers, including pericarp, lemma, aleuron and testa so that cooking takes a long time. To speed up the cooking time on rice can be modified by parboiling process so that it changes the character of gelatinization. The research aims to find out the effect of the concentration of sodium citrate solution and immersion time on the process of black rice parboiled Sirampog varieties on its gelatinization properties. Soaking in sodium citrate solution at a certain time is expected to accelerate the cooking time of black rice. The research using Completely Randomized Factorial design consisting of concentration of sodium citrate solution (0, 3, 5 and 7%) and immersion time (20, 30 and 40 minutes). The variables observed were gelatinization properties including gelatinization temperature, peak viscosity, final viscosity, breakdown viscosity, trough viscosity and setback viscosity. The results showed that the setback viscosity and peak viscosity of Sirampog rice were affected by the concentration of sodium citrate. The sodium citrate solution of 5% as a marinade produces Sirampog varieties of parboiled rice with high trough viscosity and lowest setback viscosity, which means it is easier to cook and more resistant to retrogradation during cooling.

Effect of soaking and extrusion on functional and pasting properties of cowpeas flour

Cowpea (Vigna anguiculata) is a leguminous crop that is widely grown and consumed in Asia and Africa as a major source of dietary protein. This study investigated the effect of varying extrusion conditions (temperature and moisture content) on the functional and pasting properties of cowpea flour. Particle size distribution, functional and pasting properties of cowpeas flour were determined. Moisture content had a significant effect on the functional and pasting properties of cowpeas flour followed by temperature. Increasing moisture content from 10% to 15%, increased the bulk density from 0.4 to 0.5, Oil Absorption Capacity 149 to 238, Water Absorption Capacity 407 to 422, Swelling Power 4.6 to 4.9, Water Absorption Index 3.9 to 4.2, as well as Peak & Breakdown viscosities. On the other hand, reducing the moisture content decreased Water Solubility Index (WSI) from 31 to 21. Lower extrusion temperature increased all the functional properties except WSI along with pasting properties namely Peak, Final and setback viscosities. Higher extrusion temperature increased WSI, breakdown, peak time, and pasting temperature. The results suggest that variation of extrusion conditions can be used to modify the functional and pasting properties of cowpea flour to suit different applications.

Nutritional, functional, and pasting properties of maize meal-sprouted soybean flour enriched with carrot powder and sensory properties of the porridge.

Maize meal fortification with plant protein enrich meal with adequate nutrients. The study aimed to investigate the nutritional, functional and pasting properties of composite flour samples of sprouted soybean flour (SSF) and maize flour (MF) in different ratios (0–100%) and 5% of carrot powder (CP) and sensory properties of porridges produced from the formulated flours. The ash (0.1–1.1 g/100 g), fat (3.5–7.0 g/100 g) and protein (6.3–15.8 g/100 g) contents of the flour blends significantly increased with the addition of SSF (10 – 30%) and CP (5%). The addition of SSF and CP to MF significantly increased the oil and water absorption capacity of the flour blends. The peak viscosity (1850.00–350.00 cP), breakdown viscosity (520.00– 90.00 cP) and setback viscosity (3880.50– 580.00 cP) significantly (p < 0.05) decreased with increasing the SSF. Porridge with 20, 25 and 30% SSF and 5% CP were acceptable by the panellists.

Heat-induced amorphous aggregates assembly of soy protein modulate in vitro digestibility of potato starch

This research focused on the characteristics of amorphous aggregates derived from soy protein (SPAA), and their effects on the structural, physicochemical, and digestive properties of potato starch (PS). The SPAA induced by different heating temperatures at pH 7.0 formed an inhomogeneous spherical structure. The presence of SPAA could improve the degree of short-range order of starch, increase thermal stability, reduce pasting viscosity and breakdown, and setback viscosity values of PS. For the PS complexed with SPAAs under simulated cooking conditions, the fraction of digested starch at 300 min (C300) decreased by 6–14 %, and rapid digestible starch content (RDS) decreased by 18–25 %, while the slowly digestible starch (SDS) and resistant starch (RS) increased by 0.4–3 % and 15–23 %, respectively. The SPAA at higher temperature treatment (SPAA130) reduced digestive rate coefficient (k) values more significantly than SPAA at a lower temperature (SPAA70, SPAA90, SPAA110). And the SPAA had no inhibitory effect on α-amylase. The results of this study would significantly contribute to expanding the theoretical information about protein regulation in starch digestion and promoting the development of healthy foods with digestion-resistant properties.

Relationship among gelatinization, retrogradation behavior, and impedance characteristics of potato starch

In this study, the physicochemical properties of potato starch from different varieties were investigated. Furthermore, the relationships among gelatinization, retrogradation behavior, and impedance characteristics of potato starch gels were evaluated by texture analysis, low-field nuclear magnetic resonance spectroscopy, and electrical impedance spectroscopy. The results indicated amylose content was positively correlated with setback viscosity, and negatively correlated with To and ΔH. In addition, impedance values of potato starch gels differed in a frequency-dependent manner. Notably, higher frequencies resulted in low diffusion of ions in prepared gels, which combined with the concentration of mobile ions in free water, led to a gradual decrease in impedance module. Compared with phase values, impedance module showed high correlation with gelatinization parameters (To, Tp, and Tc) and viscosity parameters (peak temperature and setback viscosity), more notably at frequencies below 100 Hz. In this context, the electric current flowed through mobile ions that interacted with bound water attached to the starch molecules at lower voltage frequencies, and were repressed by the formation of an ordered and compact gel network during retrogradation. Collectively, these results indicate that impedance spectroscopy can be potentially used as an efficient and reliable method to predict gelatinization and retrogradation behavior of potato starch.

Enhancement of Digestion Resistance and Glycemic Control of Corn Starch through Conjugation with Gallic Acid and Quercetin Using the Free Radical Grafting Method

The objective of this study was to synthesize different polyphenol–corn starch complexes including gallic acid–starch and quercetin–starch by conjugating corn starch with gallic acid and quercetin using the free radical grafting method. This process was effective in enhancing conjugations of starch molecules with gallic acid and quercetin (5.20 and 5.83 mg GAE/g, respectively) and imparted promising antioxidant capacity to the phenolic–starch complexes. Significant interactions between these phenolic compounds and corn starch molecules were revealed with an ultraperformance liquid chromatography electrospray ionization Q-time-of-flight mass spectrometry assay. It was revealed that significantly higher levels of resistant starch in the above gallic–starch and quercetin–starch complex samples (11.6 and 15.3 g/100 g, respectively) together with an obvious reduction in glycemic response (7.9% and 11.8%, respectively) observed over the control. Complex samples functionalized with gallic acid and quercetin have exerted modified physicochemical properties, particularly reduction in swelling ability (58.7–60.1%), breakdown viscosity (62.5–67.8%), and setback viscosity (37.7–44.5%). In sum, free radical grafting treatment could be a promising method for imparting corn starch with enhanced resistance to enzyme digestion along with changes in pasting properties for specific food applications.

Nitrogen fertilizer affects starch synthesis to define non-waxy and waxy proso millet quality

Understanding the effect of nitrogen fertilization on the quality of proso millet is key to expanding the use of this crop to address water scarcity and food security. Therefore, this study determined the impact of nitrogen fertilization on the proso millet quality. Nitrogen fertilization significantly increased the NR and GS activities and decreased the GBSSase activity, resulting in an increase in protein content and reduction in amylose content and L*, which decreased the appearance quality. Nitrogen fertilization increased the proportion of short amylopectin chains, resulting in a more disordered carbohydrate structure, and decreased the proportion of hydrophilic functional groups, contributing to an increase in setback viscosity and decrease in pasting temperature in the waxy (w139) variety. In contrast, the non-waxy (n297) variety exhibited a larger proportion of long amylopectin chains, lower ordered structure and hydrophobic functional groups after nitrogen fertilization, which strengthened the inter- and intramolecular forces of starch colloids.

Characterisation of Pasting, Structural and Volatile Properties of Potato Flour

Potato flour is an important raw material for potato staple food products; nevertheless, the quality and flavor vary significantly due to process changes. In this study, the physicochemical features of fresh and five different dehydration temperature potato samples, including the degree of starch gelatinization (DG), pasting, structure properties and volatile components, were compared to investigate the effect of hot air drying (HAD) on potato flour. The results showed that the degree of pasting, viscosity and volatile aroma components changed significantly with differences in drying temperature. With the increase in drying temperature, the gelatinization degree and peak viscosity of potato powder increased or decreased, the breakdown viscosity of HAD-50 was higher, the setback viscosity of HAD-90 was higher, while the crystallization zone of HAD-90 was destroyed due to the high temperature. The flavor components of potato flour are increased during processing due to lipid oxidation, Maillard reaction and thermal degradation. The level of aldehydes,3,5-Octadien-2-one and E,E)-3,5-Octadien-2-one gradually reduced as the processing temperature increased, while the content of furans grew and then decreased, nonanal and 2-Penty-l-Furan increased. Overall, lower HAD temperatures are beneficial for the quality and flavor of potato flour. The information presented here will be useful for the further development of potato flour products.

Characterization of multi-scale structure and physicochemical properties of starch from diverse Japonica waxy rice cultivars

Eight Japonica glutinous rice cultivars, collected from seven different provinces in China, were selected to explore the structural and physicochemical properties of their starch. The apparent amylose content ranged from 0.07% to 1.5%. Of parameters related to molecular structure, aggregation (crystalline, short-range order and lamellar structure) and granule structure, the amylopectin A chain (DP 6–12) and B2 chain (DP 25–36), the crystallinity and the average repeat distance of lamella of the starch granule are important in influencing physicochemical properties of waxy rice starch. The A chain level of amylopectin in waxy rice starch was negatively correlated with the onset temperature, gelatinization enthalpy and setback viscosity, but positively correlated with α-amylase hydrolysis coefficient. However, the B2 chain level of the amylopectin and the crystallinity of waxy rice starch had opposite effects on these parameters. Also, higher percentage of A was correlated with less order of the lamellar structure of waxy rice starch. Waxy rice starch with smaller granule size was more easily hydrolyzed by α-amylase. The difference in multi-scale structure and physicochemical properties can contribute to the variation in the development of waxy rice starch starch-based products.

Porous corn starch obtained from combined cold plasma and enzymatic hydrolysis: Microstructure and physicochemical properties

The combined effect of cold plasma treatment and enzymatic hydrolysis was investigated on the physicochemical and microstructural properties of porous corn starch. Scanning electron microscopy (SEM) images depicted that the combined treatment led to the creation of deeper pores on the surface of starch granules. The combined treatment indicated the highest swelling power (19.49 g/g), solubility (10.08 %), specific surface area (2.97 m2/g) and total pore volume (10.47 cm3/g). According to the X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC), the combined treatment, compared with the enzymatic hydrolysis, decreased the starch crystallinity, the order of the double-helix structure, and the starch gelatinization enthalpy. The rapid visco analyzer (RVA) pasting profile revealed that the combined treatment elevated the breakdown and setback viscosities. This study indicated that cold plasma pretreatment, as a green non-thermal technology, facilitated the performance of enzymes, resulting in the production of a porous starch with a higher absorption capacity.

A study on the structural, physicochemical, rheological and thermal properties of high hydrostatic pressurized pearl millet starch

Starch in native form has limited application due to functional and physicochemical characteristics. To overcome these limitations, starch can be modified by non-thermal technologies such as high hydrostatic pressure (HHP). This study investigates high-pressure-induced gelatinization and the effect of this process on the structural, functional, morphological, pasting, thermal, physical and rheological properties of millet starch. The suspension of millet starch and water was pressurized at 200, 400 and 600 MPa for 10, 20 and 30 min to modify the starch in terms of structure, morphology, some physicochemical and rheological properties. Swelling strength and starch solubility decreased as a result of treatment with HHP. All treatments caused to increase in water holding capacity of the starch (from 0.66 % for native starch to 2.19 % for 600 MPa-30 min). Thermal analysis showed a decrease in gelatinization temperature and enthalpy of gelatinization and the pasting properties showed a decrease in the peak viscosity after HHP treatment. In addition, HHP treatment caused to increase in the hydration ability of starch by creating porosity and gaps in the granule surface and increasing the specific surface area. HHP application resulted in an increase in the peak time and pasting temperature and a decrease in breakdown and peak viscosities, final viscosity and setback viscosity in comparison with native starch of millet. The starch sample treated with 600 MPa for 30 min had the lowest syneresis and retrogradation ability. Increasing pressure and the time led to an increase in the elastic nature of the starch samples. According to the results, it is possible to increase usage area of starches in the food industry by improving its technological with HHP. This green physical technology can influence the quality parameters of starch, which can provide benefits for product machining and economic purposes.

Sulphated and carboxymethylated polysaccharides from Lycium barbarum L. leaves suppress the gelatinisation, retrogradation and digestibility of potato starch

SummaryProcessing starch with polysaccharides promotes broader applications, and it is important to consider all possible structural interactions among the molecular ingredients. In this study, polysaccharides isolated from Lycium barbarum L. were chemically modified by sulphation (SLP) and carboxymethylation (CLP), and the effects on the gelatinisation, retrogradation, and digestibility properties of the potato starch were investigated. After chemical modification, the slope of the curve between the molar mass and molecular radius of polysaccharides decreased, revealing that the relatively rigid rod‐like structure of native polysaccharide (0.78) changed to SLP's random linear chain (0.53) and CLP's spherical conformation (0.23), and the water solubility increased from 63.6% to 70.4% (SLP) and 84.1% (CLP) in distilled water at 25 °C. Moreover, testing polysaccharides as an additive indicated that both derivatives significantly decreased the peak viscosity, breakdown, and setback viscosity during potato starch pasting. Furthermore, the possible interactions could be further examined by structure analyses, suggesting the polysaccharide and SLP hydrogen bonds interacted with the surfaces of starch granules, while CLP penetrated the interiors of pasted starch granules, destroyed the helixes and backbone structure of starch, and formed type V crystallinity with amylose. The results of in vitro digestion analysis revealed that polysaccharides could decrease the amount of resistant starch and increase the amount of slowly digestible starch, which might be related to the inhibition of starch gelatinisation. These results facilitate the application of chemical derivatives in starch‐based functional foods and understand the interaction mechanism between potato starch and polysaccharides.

Effects of post-silking low temperature on the starch and protein metabolism, endogenous hormone contents, and quality of grains in waxy maize.

Waxy maize has many excellent characteristics in food and nonfood industries. However, post-silking low temperature (LT) has severe limitations on its grain yield and quality. In this study, field and pot trials were conducted to investigate the effects of post-silking LT on the physiological, biochemical, and functional characteristics of two waxy maize grains. The field and pot trials were performed with sowing date and artificial climate chamber, respectively, for LT treatment from silking stage to maturity. Results in pot trial were used to explain and validate the findings in field trial. Compared with the ambient treatment, the LT treatment significantly reduced kernel weight during the grain filling stage (P < 0.05). LT treatment in both environments resulted in an average decrease in dry weight of SYN5 and YN7 at maturity by 36.6% and 42.8%, respectively. Enzymatic activities related to starch and protein biosynthesis decreased under the LT treatment during the filling stage, accompanied by a decrease in the accumulation amounts and contents of soluble sugar and starch, and a decrease in protein accumulation amount. Meanwhile, the contents of abscisic acid, indole-3-acetic acid, and gibberellin 3 in grains decreased under the LT treatment during the filling stage. Peak, trough, breakdown, final, and setback viscosities of grains decreased by LT. LT treatment decreased the gelatinization enthalpy of grains and increased the retrogradation percentage. In conclusion, post-silking LT stress altered the content of grain components by inhibiting the production of phytohormones and down-regulating the enzymatic activities involved in starch and protein metabolism, which resulted in the deterioration of grain pasting and thermal properties.

Deciphering multi-scale structures and pasting properties of wheat starch in frozen dough following different freezing rates

Starch is the main composition of frozen dough. How the freezing rate of frozen dough affected multi-scale structures and functionalities of starch in the frozen dough and the underlying mechanism are not systematically discussed yet. Both rapid and slow freezing treatment (0.20–1.50 ℃/min) increased the content of amylose, helical structures, crystalline structures, and short-range ordered structures and the thickness of crystalline lamellae and semi-crystalline lamellae, and thus increased swelling power, solubility, peak viscosity, breakdown viscosity, final viscosity, and setback viscosity of starch during heating. A slow freezing treatment (0.20 ℃/min) contributed to the formation of large ice crystals, and in turn, resulted in great changes in starch structures and functionalities. The corrosion of starch granules and the leach of amylose and small amylopectin during the thawing of ice crystals may be responsible for the changes in structures, swelling power, solubility, and pasting properties of starch in the frozen dough.