Serum Vitamin B12 Levels Research Articles

Influence of metformin intake on serum vitamin B12 levels in patients with type 2 diabetes mellitus.

To describe the prevalence of vitamin B12 deficiency among Jordanian patients with type 2 diabetes mellitus treated with metformin and to compare the findings with those who did not receive metformin. Total 155 patients with type 2 diabetes mellitus, aged between 48 and 82 years were enrolled in the current study. They were divided into two groups; the first (n = 120) was treated with metformin while the second (n = 35) was not. Patients' demographics (age, gender, duration of type 2 diabetes mellitus, smoking status), medication parameters (daily dosage and duration of metformin therapy), and biochemical parameters (hemoglobin level, mean corpuscular volume (MCV), serum vitamin B12, and folate level) were recorded. Definite deficiency was defined as serum vitamin B12 levels of < 150 pg/ml, whereas < 200 pg/ml indicated possible deficiency. The mean serum ± standard deviation (SD) vitamin B12 level was significantly lower in patients who were treated with metformin (268.5 ± 35.8 vs. 389.5 ± 29.8 pg/ml, p = 0.029). The metformin group had significantly higher prevalence of definite deficiency (32% vs. 9%, p < 0.02) and possible deficiency (48% vs. 30%, p < 0.02). Within the metformin group, the mean serum ± SD vitamin B12 level was significantly lower in those on high dosage (175.2 ± 30.5 vs. 315.6 ± 37.8 pg/ml, p < 0.001). MCV (μm3) levels ± SD were higher in the metformin group (87.5 ± 2.9 vs. 83.7 ± 2.4) with no statistical significance. There is a significant association between metformin intake and vitamin B12 deficiency. Serum vitamin B12 levels should be checked by physicians and serial monitoring is necessary in patients who are treated with metformin.

The role of the deficiency of vitamin B12 and folic acid on homocysteinemia in children with Turner syndrome

Premature atherosclerosis and ischemic heart disease represent a major cause of comorbidities among children with Turner syndrome. The identification of non-traditional risk aspects is crucial for the early identification and management of such comorbidities through establishing effective preventive measures. The aim of the study is to explore the role of the deficiency of vitamin B12, folic acid and homocysteine in children withTurner syndrome. The study included 78 children with Turner syndrome and 67 healthy age and sex matched children. Karyotype was implemented for all patients. The serum levels of vitamin B12, folic acid and serum homocysteine were assessed. The prevalence of the deficiency of vitamin B12 and folic acid was estimated to study its correlation to hyperhomocysteinemia in Turner syndrome children. The karyotype analysis showed 45,X (monosomy X) in the 78 patients. Vitamin B12 and folic acid were significantly decreased in children with Turner syndrome in 65-73% of the patients, respectively, while the serum level of homocysteine significantly increased to 48.7% compared to healthy controls. Homocysteine level negatively correlated with vitamin B12 and folic acid. The deficiency of vitamin B12 and folic acid increased the risk of hyperhomocysteinemia in children with Turner syndrome (OR 2.49 and 2.36, respectively). This report highlights that hyperhomocyste-inemia in children with Turner syndrome may be related to the deficiency vitamin B12 and folic acid.

An assessment of serum vitamin B12 and folate in patients with Crohn’s disease

Crohn's disease is a chronic inflammatory condition that can involve any area in the gastrointestinal tract often involving the distal ileum where vitamin B12 is specifically absorbed. The aim of this study was to ascertain serum vitamin B12 and folate levels in order to investigate the correlation among these vitamin levels and disease activation, localization, duration and age at the onset of the disease. Study population included 103 patients with Crohn's disease and a healthy control group of 114 individuals. C-reactive protein, vitamin B12, folate levels were studied along with hemogram analyses. The results were evaluated in statistical comparisons. While serum vitamin B12 levels and serum folate levels were 161.9 ± 63.2(73-496) pg/mL and 4.9 ± 1.4(1.2-9.4) ng/mL in the Crohn's patient group respectively, they were 321.7 ± 126.3(85-680) pg/mL and 7.6 ± 3.8(3-25.1) ng/mL in the control group respectively. Vitamin B12 and folate levels were distinctly lower in patients with Chron's disease than those of the control group (P < .001). The intragroup analysis of the patient group revealed that low vitamin B12 levels were significantly lower in the moderate group classified according to the Crohn's Disease Activity Index (P < .001), along with those in the L1 group with terminal/distal ileal involvement (P < .001). Vitamin B12 and folate deficiencies are quite prevalent in patients with Crohn's disease while this condition can lead to various complications and they prove to be important risk factors associated especially with thrombosis and its complications. Patients must be regularly followed-up for vitamin B12 and folate levels to supplement them where needed.

COMPARATIVE OBSERVATIONAL STUDY OF SERUM VITAMIN B12 LEVELS IN NEWLY DIAGNOSED TYPE 2 DIABETES MELLITUS AND NON-DIABETIC PATIENTS

BACKGROUND:&#x0D; Methylcobalamin or Vitamin B12 is a coenzyme involved in the synthesis of methionine, pyrimidine and purine bases. Type 2 diabetes is associated with oxidative stress; Vitamin B12 deficiency in diabetic patients taking metformin has been linked with oxidative stress. Because of these associations, it is implied that Vitamin B12 deficiency must be considered a risk factor for complications related to diabetes. However, several studies also suggest that deficiency of Vitamin B12 starts from the early stages of Diabetes. It has also been suggested that Vitamin B12 supplementation may help in reducing the complications related to diabetes. Although, the use of prophylactic Vitamin B12 to reduce complications related to diabetes remains controversial.&#x0D; Objective: This study aimed to explore the clinical relevance of Vitamin B12 in newly diagnosed T2DM patients. This study measured Vitamin B12 levels in newly diagnosed patients with type 2 diabetes (T2DM) and explored the correlations between its serum levels and various metabolic parameters in T2DM.&#x0D; RESEARCH DESIGN AND METHODS&#x0D; This was a 1 year, single-center, prospective, comparative, observational study. To collect data on age, smoking history, alcohol consumption, physical activity status, family history of T2DM, and medications, all subjects completed a self-answered questionnaire. Plasma samples were used to assay the biochemical parameters such as FBS, PPBS, HbA1c, lipid profile, and serum Vitamin B12. The participants were divided into newly diagnosed T2DM patients (n = 300) and healthy control subjects (n = 300).&#x0D; RESULTS&#x0D; Serum Vitamin B12 was significantly lower in newly diagnosed T2DM patients than in healthy control subjects (362.07± 182.25 vs 559.23± 275.05 pg/mL; P &lt; 0.001). Serum Vitamin B12 correlated negatively with FBS, PPBS, and HbA1c in newly diagnosed T2DM patients.&#x0D; CONCLUSION&#x0D; Serum Vitamin B12 levels are significantly decreased in newly diagnosed T2DM patients. Our results suggest that adding Vitamin B12 as a supplement in the early stages of T2DM may contribute to possible microvascular (neuropathy, retinopathy, nephropathy, etc. ) complications related to Type 2 Diabetes Mellitus.

Vitamin B12 Status and Gut Microbiota among Saudi Females with Obesity.

Previous studies have suggested that dietary habits and dysbiosis of gut microbiota contributed to obesity development. Vitamin B12 is produced by microbes; however, the relationships between vitamin B12, gut microbiome, and obesity are understudied. We aimed to determine the association between vitamin B12 status and gut microbiota relative to obesity in 92 Saudi Arabian females aged 19-25 years who were obese (n = 44) or normal weight (n = 48). Anthropometric, biochemical data, and dietary data were collected. The microbial communities of stool samples were characterized using the shotgun metagenomic sequencing technique. The relationship between vitamin B12 status and gut microbiota composition was identified using Pearson correlation analysis. A statistically significant difference was found in bacterial α- and β-diversity between the groups relative to median serum vitamin B12 level (404.0 pg/mL) and body weight. In the total participants, dietary vitamin B12 intake was inversely correlated with Bifidobacterium kashiwanohense and Blautia wexlerae species. In obese participants, dietary vitamin B12 intake was inversely correlated with Akkermansia muciniphila species and species from the Verrucomicrobia phylum, whereas it was positively correlated with Bacteroides species. Our findings indicate that the abundance (frequency) and diversity (richness) of gut microbiota are associated with vitamin B12 levels and obesity in young females.

Electrophysiological profile of serum vitamin B12 levels, correlation with serum methylmalonic acid levels, and determination of subclinical peripheral nerve involvement

Background/Aim: Vitamin B12 is essential for normal neural conduction in peripheral nerves. This study aimed to investigate the electrophysiological profile for varying degrees of serum B12 levels and to determine whether a correlation existed between electrophysiological profiles and serum methylmalonic acid (MMA) levels. Also, determination of subclinical peripheral nerve involvement with cold administration was planned in serum B12 levels. Methods: A total of 101 (63 females, 38 males) subjects with known serum vitamin B12 levels were selected randomly from the neurology outpatient clinic for this study. The subjects were divided into three groups based on the serum total Vitamin B12 levels: (1) B12 deficiency (&lt;126 pg/mL), (2) low B12 (126-250 pg/mL), and (3) normal B12 (250–500 pg/mL). Serum MMA and nerve conduction studies (NCS) were assessed and compared between the three groups. After the cooling procedure was applied to the ulnar and sural nerves, NCS was repeated. Results: There were 13 subjects in the B12 deficiency group, 44 subjects in the low B12 group, and 44 subjects in the normal group. We found that ulnar sensory nerve action potential amplitudes were significantly decreased (P = 0.009), ulnar F latency (P = 0.003; P &lt; 0.001)) was prolonged, and peroneal combined muscle action potential amplitudes decreased (P = 0.026) in the B12 deficiency when compared with the low and normal B12 groups. Sural nerve amplitude and conduction velocities were found to be significantly abnormal after the cold application in all groups (P &lt; 0.001). The increase in sural nerve sensory nerve amplitude potentials (SNAP) amplitudes was higher in the B12 deficiency group than in the other groups. Mean serum MMA levels were high in all groups. A correlation of nerve conduction study (NCS) changes with serum vitamin B12 and MMA was not observed in the groups after cold application. Conclusion: Vitamin B12 deficiency may cause subclinical sensorial and motor axonal nerve conduction changes. Nerve conduction changes may not always reach pathological values based on electrophysiological studies but may be detected after cooling administration even in the normal serum B12 levels. A correlation between serum MMA and vitamin B12 levels was found. Therefore, serum levels of vitamin B12, which is important for nerve conduction, should be carefully evaluated in clinical practice.

STUDY OF VITAMIN B12 LEVELS IN PATIENTS WITH TEMPORARYDEFUNCTIONING ILEOSTOMY- A CASE SERIES

Pain abdomen is one of the the most common presentations of Ileal perforation. In emergency setting, ileostomy is the most commonly performed procedure for damage control and ileostomy closure is usually planned in the long run usually within 3 months. Naturally, after ileostomy there is alteration in the normal homeostasis of nutrient absorption in the gut. Vitamin B12 is the one of the most important micronutrient absorbed in the ileum.This study focuses on presence of any correlation between serum vitamin B12 levels and its absorption in patients of Temporarydefunctioning ileostomy over the period of time.

The Effects of Serum Folic Acid and Vitamin B12 on the Risk of Gestational Diabetes Mellitus.

In order to gain more knowledge on the risk of gestational diabetes mellitus (GDM), and to provide evidence for clinical guidance on the optimum level of serum folic acid and vitamin B12, this study aimed to clarify the relationship between serum folic acid and vitamin B12 and the risk of GDM. This retrospective case-control study was conducted based on the clinical information system of the Maternal and Child Health Hospital of Hubei Province. Clinical data including maternal socio-demographical characteristics, serum folic acid, and vitamin B12 were collected. Logistic regression analyses and restricted cubic splines were performed to examine the impact of serum folic acid and vitamin B12 on the risk of GDM. Significantly elevated risks of GDM were observed in groups with high serum folic acid concentration (OR = 1.84, 95% CI: 1.07-3.16), and in low vitamin B12 concentration (OR = 2.14, 95% CI: 1.26-3.65). After stratified by age, the increased risk of GDM was still noticed in a low level of vitamin B12 among mothers aged <30 years (OR = 4.76, 95% CI: 1.45-15.61). In mothers with pre-pregnancy BMI <24, elevated risk of GDM was significantly associated with a high folic acid (OR = 2.09, 95% CI: 1.11-3.93) or a low vitamin B12 concentration (OR = 2.24, 95% CI: 1.22-4.14). Moreover, the risk of GDM was on the decline with the increased level of folic acid in the beginning, and it started to manifest an upward trend when the serum folic acid reached 19.02 ng/mL. This study demonstrated that serum folic acid excess or vitamin B12 deficiency could contribute to the increased risk of GDM, and revealed the potential side effect of serum folic acid overdose. As serum folic acid and vitamin B12 tests are widely applied in clinical practice, this finding could help clinicians to evaluate maternal risk from a new perspective.

Nutritional status, nutrient imbalances, food-related behaviors and dietary supplements use among patients with celiac disease on a gluten free diet in Lebanon: a national cross-sectional study

Background: Celiac disease is an autoimmune disorder triggered by gluten, that occurs in susceptible individuals and is associated with dietary restriction and subsequent nutritional deficiencies. This study investigated the diet quality, nutrition imbalances and nutrition status among young children,adolescents and adults with CD who followed a gluten free diet an who were referred to several hospitals in Lebanon.Methods:A cross-sectional study in 50 individuals (31.74 ± 15.64 years) with CD was conducted, using biochemical parameters, anthropometric measurements, dietary and physical activity assessments.Results: Of the 50 participants, 38% and 16% were presenting low serum levels of iron and vitamin B12, respectively. The majority of participants were physically inactive and around 40% of them had low muscle mass. A weight loss of 10% to 30% indicating mild to moderate malnutrition was shown in 14% of individuals. The assessment of food-related behaviors shows that 80% of participants were reading nutrition labels and 96% of them were following gluten-free diets. Some barriers including family ignorance (6%), language of the nutrition labels (20%) and expensive GF products (78%) were limiting the adherence to GFD. The inadequacy of the daily energy intake along with insufficient intakes of calcium and vitamin D were remarked among individuals with CD. However, protein and iron intake were exceeding the recommendations among all age groups, except in males aged 4-8 years and 19-30 years. Half the study participants were using dietary supplements where 38%, 10%, 46%, 18%, 16% and 4% used vitamin D, vitamin B12, iron, calcium, folate and probiotics, respectively.Conclusion: GFD is the key treatment for CD. However, it is not without inadequacies and may cause certain deficiencies such as calcium and vitamin D leading to reduced bone density. This underlines the critical role of dietitians in education and maintenance of healthy GFD among individuals with CD.

Zinc Deficiency: The Association with Vitamin B12 in People of Different Ages

Background: Zinc and vitamin B12 are the foremost important micronutrients. About a quarter of Iranian households have a zinc deficiency. Objectives: Considering the importance of these micronutrients, this study aimed to investigate the association between zinc deficiency and vitamin B12 in people of different ages who had their blood tested in the Pasteur laboratory in Ahvaz. Methods: In this cross-sectional study, serum levels of zinc and vitamin B12 were measured using atomic absorption spectrophotometry and electro-chemiluminescence, respectively. Then, samples were divided into normal and zinc-deficient groups based on serum zinc levels. Serum vitamin B12 levels were also compared between two defined groups. The correlation between age and both zinc and vitamin B12 were determined through statistical tests. Results: In 60.5% of samples, serum zinc was lower than in the normal group. However, the two groups had no statistically significant difference in zinc levels (P = 0.085). Findings showed that with increasing one year of age, serum zinc level decreases by 0.13 units, and serum level of vitamin B12 increases by 0.19 units. Although the serum level of vitamin B12 in the group with normal zinc levels was higher than in the zinc-deficient group, this difference was not statistically significant (P = 0.743). Conclusions: Zinc and Vitamin B12 serum levels can have a clinically positive correlation, and zinc deficiency might be effective in manipulating vitamin B12 status in the body.

Micronutrients Deficiencies in Candidates of Bariatric Surgery: Results from a Single Institution over a 1-Year Period

BackgroundMicronutrient deficiencies represent a common condition after bariatric surgery (BS). The prevalence of these nutritional disorders before BS is still debated. The aim of our study was to retrospectively evaluate the prevalence of micronutrient deficiencies in candidates for BS.MethodsA prospectively maintained database of our institution was searched to find all patients who underwent surgery between January and December 2021. The following data were collected: age, gender, body mass index (BMI), obesity-associated diseases, and preoperative serum levels of vitamin B12, folate, and vitamin D.ResultsA total of 174 patients were included in our study. Mean age and BMI were 39.2 ± 11.4 years and 44.3 ± 7.1 kg/m2, respectively. One hundred and thirty-nine patients (79.9%) had at least one preoperative micronutrient disorder, with vitamin D deficiency being the most common (116, 66.7%), followed by a deficit of folate (76, 43.7%) and vitamin B12 (10, 5.7%). Forty-seven (27%) individuals had insufficient levels of vitamin D. Comparison of deficiencies between sexes showed that vitamin B12 < 20 ng/ml was significantly more frequent in women (p = 0.03). DLP showed a mild significant effect on folate levels (p = 0.01), while the association of HNT and T2DM had a mild significant effect on vitamin B12 (p = 0.02).ConclusionsPreoperative micronutrient deficiencies were frequently found in candidates for BS. Approximately 90% of patients had deficient or insufficient serum levels of vitamin D preoperatively. Almost half of the patients had a preoperative deficit of folate, and vitamin B12 deficiency was significantly more frequent in the female population. It is mandatory to screen all patients undergoing BS for vitamin deficiencies before surgery.

Higher frequencies of anemia, hematinic deficiencies, and gastric parietal cell antibody positivity in vitamin B12-deficient Taiwanese male oral submucous fibrosis patients

Oral submucous fibrosis (OSF) is a progressive fibrotic oral mucosal disease associated with betel quid chewing. This study evaluated whether Taiwanese male OSF patients with vitamin B12 deficiency (the serum vitamin B12 level ≤ 450pg/mL, B12-deficient OSF patients) had high frequencies of blood hemoglobin (Hb) and serum iron and folic acid deficiencies, and serum gastric parietal cell antibody (GPCA) positivity. The blood Hb and serum iron, vitamin B12, folic acid, and GPCA concentrations in 66 Taiwanese male B12-deficient OSF patients were measured and compared with the corresponding data in 132 age-matched healthy male control subjects. We found that 6 (9.1%), 19 (28.8%), 35 (53.0%), and 9 (13.6%) of the 66 B12-deficient OSF patients had blood Hb (<13g/dL) and serum iron (≤70μg/dL), and folic acid (≤6ng/mL) deficiencies, and serum GPCA positivity, respectively. Furthermore, 66 OSF patients had significantly higher frequencies of blood Hb and serum iron and folic acid deficiencies, and serum GPCA positivity than 132 healthy control subjects (all P-values < 0.05). Of the 6 anemic B12-deficient OSF patients, one had macrocytic anemia, two normocytic anemia, and three thalassemia trait-induced anemia. Taiwanese male B12-deficient OSF patients have high frequencies of blood Hb, serum iron and folic acid deficiencies, and serum GPCA positivity. The anemia and hematinic deficiencies in B12-deficient OSF patients are likely due to OSF symptoms and signs-caused insufficient intake, poor chewing, and malabsorption of hematinic elements from ingested food stuffs rather than the GPCA positivity.

Evaluation of serum vitamin B12 levels and its correlation with clinical presentation in patients with trigeminal neuralgia.

The objective of this study was to estimate serum vitamin B12 levels and its correlation with severity of clinical presentation in patients with trigeminal neuralgia (TN). A total of 80 participants were included and rendered into study group: 40TN patients and control group: 40 healthy individuals. The serum vitamin B12 estimation of each participant was carried out by using serum vitamin B12 ELISA kit and pain characteristics of TN patients were recorded in details. The mean serum vitamin B12 level was noticed to be significantly lower (p=0.042) in study group (296.87±248.75pg/mol) as compared to control group (423.5±296.41pg/mol). There was a statistically significant difference in serum vitamin B12 level between vegetarian TN patients and those who were on mixed diet (p=0.001). The pain associated with TN predominantly reported unilaterally involving the right side (55%), female gender (55%) and mandibular division (50%). Although pain intensity showed no significant relation (p=0.024), duration of pain reported a strong negative association with mean serum vitamin B12 levels in TN patients (p=0.001). Vitamin B12 supplements can be added to the established treatment protocol for the holistic management of TN patients particularly those who are on vegetarian diet.

Association of Vitamin B Status with Risk of Dementia in Cohort Studies: A Systematic Review and Meta-Analysis.

To examine the association between B vitamins status and the risk of dementia in older adults through a systematic review and meta-analysis of cohort studies. Systematic review and meta-analysis. Older adults aged ≥60years from community, nursing home, institution, or hospital. PubMed, Cochrane Library, EMBASE, Web of Science, CINAHL, ClinicalTrials, WHO-ICTRP, NHS Trusts, and ACTR were searched from the date of their inception up to November 28, 2021. We included cohort studies that assessed the association between serum B vitamins or B vitamins intake and the risk of dementia among older adults aged ≥60years. The quality of all studies was assessed by the modified Newcastle-Ottawa Scale (NOS). The hazard ratios (HRs) and 95% CIs were calculated by the random effects model. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach was used to rate the certainty of evidence. Eleven cohort studies with sample sizes ranging from 233 to 3634 were included in the meta-analysis. Levels of serum folate showed statistically significant association with the risk of dementia (≥10nmol/L: HR 1.57, 95% CI 1.01-2.46, low certainty; &lt;10nmol/L: HR 0.93, 95% CI 0.88-0.99, very low certainty). However, levels of vitamin B₆ intake showed no statistically significant effects on risk of dementia; levels of serum vitamin B₁₂ and vitamin B₁₂ intake also showed no statistically significant effects on risk of dementia in older adults. The results from our meta-analysis suggest that vitamin B₆, B₁₂, and folate may not be modifiable risk factors for dementia among older adults. Current evidence on the relationship between vitamin B status and dementia is not sufficient for development of vitamin B recommendations. More high-quality cohort studies are needed to confirm the relationship between the two in the future.

PSUN315 Elevated Serum B12 Levels are Associated with Diabetes

Abstract Background Vitamin B12 is an essential nutrient found in diverse food sources. Prokaryotic beings are capable of production through de novo aerobic and anaerobic biochemical reactions. It is common wisdom that diabetics, especially those receiving metformin, have vitamin B12 deficiency. However, little is known about the relationship between B12 levels and glucose tolerance. Methods A retrospective analysis of vitamin B12 levels and glucose tolerance in a representative U.S. population was performed. Records from the 2011-2014 National Health and Nutrition Examination Survey were reviewed. Individuals over the age of 20 were grouped according to a history of diabetes, Hb A1c levels, and fasting or 2-hour glucose tolerance test results into normal glucose (NG), abnormal glucose tolerance (AGT), and diabetes. A total of 19, 931 individuals were identified. Sample weighted analyses were performed with adjustment for the covariates of age, gender, body mass index, race/ethnicity, education, poverty index, current smoking, current alcohol consumption, family history of diabetes, vitamin B12 intake, and metformin use. Results Serum vitamin B12 levels were significantly higher among individuals with diabetes compared to those defined as AGT and NGT patients (496±14, 453±6, and 443±8 pmol/L, mean ± SE respectively, P=0.004). Diabetic individuals taking metformin had significantly lower serum vitamin B12 levels than diabetics not using metformin (445±16 vs. 518±13 pmol/L, P=0.0005). After exclusion of metformin users, diabetic subjects still had significantly higher serum vitamin B12 levels than NGT and AGT individuals (498±15, 456±6, and 445±8 pmol/L, respectively, P=0.006). The frequency of vitamin B12 supplement use was similar among diabetic, NGT, and AGT subjects (31%, 33%, and 31%, respectively, P=0.30). Likewise, the quantity of vitamin B12 supplementation was similar among the groups (233±34, 189±18, 192±24 mcg/day, respectively, P=0.50). Summary Retrospective analysis found higher serum B12 levels in diabetics compared to individuals with abnormal and normal glucose homeostasis. This novel finding was not secondary to exogenous B12 supplementation in diabetic individuals. Not unexpectedly, in individuals with diabetes, metformin exerted a negative effect on B12 levels. While the causes of elevated B12 in these individuals remain unknown, analysis of the intestinal bacterial flora and metabolome may be warranted. Presentation: Sunday, June 12, 2022 12:30 p.m. - 2:30 p.m.

Vitamin B12 deficiency may play an etiological role in atrophic glossitis and its grading: A clinical case-control study

BackgroundExisting studies have reported the significant association between atrophic glossitis (AG) and hematinic deficiencies, including iron, folate and vitamin B12 deficiency. However, these findings were inconsistent. AG can be graded as partial or complete atrophy. It is still unclear whether hematinic deficiencies are associated with the grading of AG.Methods236 AG patients and 208 sex- and age-matched healthy controls were enrolled in this study. Hematological tests including complete blood count, and serum levels of folate, ferritin and vitamin B12 were performed. The AG group was divided into those with partial AG and those with complete AG according to the extent of papillary atrophy. Statistical analysis was performed to assess whether hematinic deficiencies are risk factors for AG and its grading.ResultsCompared with the healthy controls, AG patients had significantly higher frequencies of vitamin B12 deficiency (68.22%), ferritin deficiency (13.98%) and anemia (21.61%). The differences in hematinic deficiencies and anemia between AG patients and healthy controls changed according to gender and age. The frequencies of serum vitamin B12 deficiency and anemia in the complete AG subgroup were significantly higher than those in the partial AG subgroup. Logistic regression analysis revealed that vitamin B12 deficiency and anemia were significantly correlated with AG and its grading. The AG patients with vitamin B12 deficiency responded well to supplement therapy.ConclusionAG could be an important clinical indicator for potential vitamin B12 deficiency, especially when the degree of tongue atrophy more than 50% and complete atrophy. Vitamin B12 deficiency might play an etiological role in the development of AG.

The effect serum iron, vitamin B12 and ferritin levels in adult patients infected with Helicobacter pylori

Objective : This study aimed to investigate the relationship between hematological parameters, serum iron, and vitamin B12 levels in adult hospitalized Palestinian patients infected with Helicobacter pylori.Methods : The collected the data, prospectively, all H.Pylori cases admitted in lab private during the study period between April 2022 and August 2022. During the five months , (30) cases with H.pylori and (30) control . patients had H.pylori positive. laboratory analysis of inflammatory indices and organ function was accomplished for the sum total of cases and controlsRESULT : The results in this research showed that there is a highly significant between stomach germs(H. pylori), iron, ferritin and vitamin B12, and there is non-significant with vitamin D.

Investigation of Serum Folate-Receptor-1 Levels in Patients with Non-Small Cell Lung Cancer

Objective: Histopathological overexpression of folate receptor-1 (FOLR1) involved in folate transport in cell growth has been reported in various cancers. Increased serum FOLR1(sFOLR1) has also been reported in epithelial ovarian cancer. The aim was to investigate sFOLR1 levels in non-small cell lung cancer (NSCLC) patients and the response prediction of the standard chemotherapy targeting folic acid metabolism.&#x0D; Method: In this prospective study, sFOLR1 levels were investigated in 30 healthy individuals and 60 patients with stage4 malign metastatic NSCLC before and after standard chemotherapy. The commercial immunoassay (ELISA) kit used for analysis of sFOLR1. Serum carcinoembryonic antigen (CEA), vitamin B12, and folate levels were also investigated. &#x0D; Result: In NSCLC patients sFOLR1 levels were significantly higher(p

Acquired Vitamin B12 Deficiency in Newborns: Positive Impact on Newborn Health through Early Detection.

The early diagnosis of and intervention in vitamin B12 deficiency in exclusively breastfed infants by mothers with low vitamin B12 is crucial in preventing possible irreversible neurologic damage, megaloblastic anemia, and failure to thrive. We assess the usefulness of the early detection of asymptomatic B12 deficiency related to acquired conditions and highlight the importance of monitoring serum vitamin B12 levels during pregnancy. We describe demographic, clinical, dietary, and biochemical data, including the evolution of a vitamin B12 deficiency’s functional biomarkers. We enrolled 12 newborns (5 males) with an age range of 1–2 months old that were exclusively breastfed and asymptomatic. These cases were referred to our metabolic unit due to alterations in expanded newborn screening: high levels of methylmalonic acid and/or total homocysteine (tHcy). All mothers were under a vegetarian diet except three who had abnormal B12 absorption, and all presented low or borderline serum B12 level and high plasma levels of tHcy. Supplementation with oral vitB12 re-established the metabolic homeostasis of the mothers. In infants, therapy with an intramuscular injection of 1.0 mg hydroxocobalamin led to the rapid normalization of the metabolic pattern, and a healthy outcome was observed. Acquired B12 deficiency should be ruled out before proceeding in a differential diagnosis of cobalamin metabolism deficits, methylmalonic acidemia, and homocystinuria.

Comparison of Serum Vitamin B12 Levels Among Drug-Naïve and Metformin-Treated Patients With Polycystic Ovary Syndrome.

Background Metformin is frequently prescribed for polycystic ovary syndrome (PCOS). There is a well-established causal relationship between metformin use in type 2 diabetes and vitamin B12 deficiency; such a relationship is seldom studied in PCOS. We conducted this study to compare vitamin B12 levels among newly diagnosed and metformin-treated patients with PCOS. Methodology This cross‑sectional comparative study was conducted from July 2021 to June 2022, among patients with PCOS attending a specialized endocrinology clinic of a tertiary hospital in Mymensingh, Bangladesh. A total of 50 patients newly diagnosed with PCOS and 52 with PCOS who had received metformin for at least six months were evaluated. The serum vitamin B12 level was measured by utilizing the chemiluminescent microparticle immunoassay principle. Results The new drug-naïve and metformin-treated subjects with PCOS had similar clinical and laboratory parameters except for the metformin group's lower hemoglobin levels and higher plateletcrit. Metformin receivers had lower serum vitamin B12 levels than the drug-naïve subjects (385.5 pg/mL [interquartile range, or IQR, 298.7-535.2] vs. 272.0 pg/mL [IQR 217.0-395.7];P< 0.001). The metformin group had higher frequencies of B12 deficiency and borderline deficiency (15.4% vs. 6% and 42.3% vs. 18%, respectively; P= 0.003). Conclusions This study observed lower serum B12 levels in PCOS patients using metformin than in the newly diagnosed ones. Large-scale data are needed to recommend routine periodic screening for B12 levels in metformin-treated PCOS.