Serum Very Low Density Lipoprotein Research Articles

Blood Sugar Concentration, HbA1C, and Lipid Profile in Heart Patients

The study included 45 samples (30 patients with heart disease and have diabetes and 15 patients with Type 2 DM), their age between (40-60) years for both gender. Samples were collected from Kirkuk general hospital from April 2023 to September 2023. The individuals of this study were divided into two groups: The first group was for patients with heart disease n (30), and the second group for individual have diabetes only n (15). Result: Heart disease patients had higher mean serum cholesterol, triglyceride, LDL, and VLDL levels (225.1±25.2, 209.5±8.3, 140.7±17.9, 41.9±4.61) compared to diabetes patients (196.3±27.2, 165±6.1, 119.67±15.2, 33.00±3.22) at a P-value < 0.05. HDL levels were similar across groups at P > 0.05. The research demonstrates the mean FBS and HbA1C of 30 heart disease and 15 diabetic patients. The study found that heart disease patients had higher mean serum FBS and HbA1C levels (282.8±36.41 mg/dl, 10.35±3.18%) compared to diabetes patients (175.2±32.79 mg/dl, 8.22±2.51%). A P-value < 0.05. Conclusion: The study concluded appositive association between dyslipidemia and heart disease in which increased cholesterol, triglyceride, low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), fasting blood glucose and HbA1C and no differences between heart disease and diabetes patients.

Compromised very-low density lipoprotein induced polyunsaturated triglyceride accumulation in N-nitrosodiethylamine-induced hepatic steatosis

N-Nitrosodiethylamine (NDEA), a carcinogen in some foods and medications, is linked to liver damage similar to non-alcoholic fatty liver disease (NAFLD). This study explores how NDEA disrupts liver lipid metabolism. Sprague-Dawley rats were given two doses of NDEA (100 mg/kg) orally, 24 h apart. Liver response was assessed through tissue staining, blood tests, and biochemical markers, including fatty acids, lipid peroxidation, and serum very-low density lipoprotein (VLDL) levels. Additionally, lipidomic analysis of liver tissues and serum was performed. The results indicated significant hepatic steatosis (fat accumulation in the liver) following NDEA exposure. Blood analysis showed signs of inflammation and liver damage. Biochemical tests revealed decreased liver protein synthesis and specific enzyme alterations, suggesting liver cell injury but maintaining mitochondrial function. Increased fatty acid levels without a rise in lipid peroxidation were observed, indicating fat accumulation. Lipidomic analysis showed increased polyunsaturated triglycerides in the liver and decreased serum VLDL, implicating impaired VLDL transport in liver dysfunction. In conclusion, NDEA exposure disrupts liver lipid metabolism, primarily through the accumulation of polyunsaturated triglycerides and impaired fat transport. These findings provide insight into the mechanisms of NDEA-induced liver injury and its progression to hepatic steatosis.

Comparison of serum very low-density lipoprotein concentrations during transition in primiparous and multiparous cows.

This study was carried out as an observational study in order to examine the difference of change in serum very low-density lipoprotein (VLDL) between primiparous and multiparous cows. Twenty-one clinically healthy cows (10 primiparous and 11 multiparous) were selected at 21 days prior to expected calving. Blood samples were collected in the morning (before feeding) on days -21, -7, 7, 21 and 56 days in milk (DIM). At 7 and 21 DIM, the serum non-esterified fatty acid concentration of multiparous cows was significantly higher than that of primiparous cows. The serum β-hydroxybutyrate concentration was also markedly higher in multiparous cows than in primiparous cows at 21 DIM. These results suggested that the degree of negative energy balance was greater in multiparous cows than in primiparous cows during this period. In both, serum VLDL concentrations decreased at over 7 DIM, increased at 21 DIM, and then decreased at 56 DIM. On the other hand, triglyceride and total protein concentrations of VLDL in multiparous cows were significantly lower than in primiparous cows at 21 DIM. This suggests that multiparous cows have poor triglyceride secretion from the liver and that they become more susceptible to hepatic lipidosis.

Rumen-protected choline reduces hepatic lipidosis by increasing hepatic triacylglycerol-rich lipoprotein secretion in dairy cows

Objectives were to determine the effects of supplementing rumen-protected choline (RPC) on hepatic composition and secretion of triacylglycerol-rich lipoprotein when cows were subjected to feed restriction to develop fatty liver. It was hypothesized that RPC reduces hepatic triacylglycerol by enhancing secretion of hepatic lipoprotein. Pregnant, nonlactating parous Holstein cows (n = 33) at mean (± standard deviation) 234 ± 2.2 d of gestation were blocked by body condition (3.79 ± 0.49) and assigned to receive 0 g/d (CON), 25.8 g/d choline ion from a RPC product containing 28.8% choline chloride (CC; treatment L25.8), or 25.8 g/d of choline ion from a RPC product containing 60.0% CC (H25.8). Cows were fed for ad libitum intake for the first 5 d and restricted to 41% of the net energy for lactation required for maintenance and pregnancy from d 6 to 13. Intake of metabolizable methionine was maintained at 18 g/d during feed restriction by supplying rumen-protected methionine. Hepatic tissue was sampled on d 6 and 13 and analyzed for triacylglycerol and glycogen, and mRNA expression of hepatic tissue was investigated. On d 14, cows were not fed and received a 10% solution of tyloxapol intravenously at 120 mg/kg of body weight to block hydrolysis of triacylglycerols in very low density lipoprotein (VLDL). Blood was sampled sequentially for 720 min and analyzed for concentration of triacylglycerol and total cholesterol. Lymph was sampled 6 h after tyloxapol infusion, and analyzed for concentrations of fatty acids, β-hydroxybutyrate, glucose, triacylglycerol, and total cholesterol. A sample of serum collected at 720 min after tyloxapol was assayed for the metabolome composition. The area under the curve (AUC) of serum triacylglycerol, VLDL cholesterol, and total cholesterol were calculated. Orthogonal contrasts evaluated the effect of supplementing RPC (CON vs. [1/2 L25.8 + 1/2 H25.8]) and source of RPC (L25.8 vs. H25.8). Least squares means and standard errors of the means are presented in sequence as CON, L25.8, H25.8. During feed restriction, supplementation of RPC reduced hepatic triacylglycerol (9.0 vs. 4.1 vs. 4.5 ± 0.6%) and increased glycogen contents (1.9 vs. 3.5 vs. 4.1 ± 0.2%). Similarly, supplementation of RPC increased the expression of transcripts involved in the synthesis and assembly of lipoproteins (MTTP), cellular autophagy (ATG3), and inflammation (TNFA), and reduced the expression of transcripts associated with mitochondrial oxidation of fatty acids (HADHA, MLYCD) and stabilization of lipid droplets (PLIN2). After infusion of tyloxapol, RPC increased the AUC for serum triacylglycerol (21,741 vs. 32,323 vs. 28,699 ± 3,706 mg/dL × min) and VLDL cholesterol (4,348 vs. 6,465 vs. 5,740 ± 741 mg/dL × min) but tended to reduce the concentrations of triacylglycerol in lymph (16.7 vs. 13.8 vs. 11.9 ± 1.9 mg/dL). Feeding RPC tended to increase the concentrations of 89 metabolites in serum, after adjusting for false discovery, including 3 acylcarnitines, 1 AA-related metabolite, 11 bile acids, 1 ceramide, 6 diacylglycerols, 2 dihydroceramides, 1 glycerophospholipid, and 64 triacylglycerols compared with CON. Feeding 25.8 g/d of choline ion as RPC mediated increased hepatic triacylglycerol secretion to promote lipotropic effects that reduced hepatic lipidosis in dairy cows.

The study of serum lipid profile in patients with cirrhosis of liver

Background: Cirrhosis of liver is a chronic condition of hepatocellular failure due to different etiology which leads multiple complications and increases the morbidity and mortality. The aim of the study was to study the lipid profile in patients with cirrhosis and to assess if it relates to the severity of the cirrhosis. Hence, abnormal lipid profile may be the surrogate marker of the severity of cirrhosis of liver. Aims and Objectives: Study lipid profiles in cirrhotic patients and compare with healthy population and to relate severity of cirrhosis of liver with the finding of abnormal lipids. Materials and Methods: This study was a cross-sectional study conducted in the department of internal medicine over a period of 6 months. It was carried on 51 cirrhotic patients and 50 non-cirrhotic subjects who attended the outpatient and inpatient department. The cirrhosis of liver was established with history, examination, and investigations. Results: The mean total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very LDL (VLDL), respectively, of non-cirrhotic subjects was found to be higher than cirrhotic patients, respectively. All lipid profiles TC, TG, HDL, LDL, and VLDL value found to be (P<0.05) which were statically significant. Serum TC, TG, HDL, LD, and VLDL level decreased more in Child-Turcotte-Pough Criteria (CTP) Class C as compared to CTP Class B. The TC, HDL, and LDL belonged cases to the CTP class C and B showed (P<0.05) found statistical significance, which can be further correlated with the severity of cirrhosis. In the present study, the level of TG and VLDL was (P>0.05) found statistically non-significant. Conclusion: All the lipid parameters decrease in cirrhotic patients as compare to non-cirrhotic subjects and progressively lower the parameters as severity of liver disease increased.

Correlation of serum protein and lipid profile with OSMF severity among the rural population of Southern India

Background: Carcinogenesis is mostly observed due to alterations in serum protein and lipid levels. Objectives: In this present study, serum protein and lipid profile are to be evaluated in OSMF patients and compared with various clinical stages of OSMF. Materials and methods: Out of 100 study subjects, 45 OSMF patients were selected as cases; 55 apparently healthy individuals were chosen as controls. The level of serum total protein, albumin, and globulin; high density lipoprotein (HDL), very low density lipoprotein (VLDL), total cholesterol (TC), triglycerides (TG), and low density lipoprotein (LDL) were measured by COBAS-6000 autoanalyzer. The obtained data were processed with an independent sample t-test. Results: Statistically significant reduction in serum total protein and albumin, LDL, HDL, TC, and TG levels were detected in all stages of OSMF patients in comparison with controls, whereas changes in serum VLDL level were not statistically significant. Conclusion: The current study showed significant alterations in serum protein and lipid profile, with advancement in the clinical stages of OSMF. In the future, the alterations in these parameters might possess a diagnostic significance and can be utilized as a biochemical indicator for the detection of neoplastic processes like OSMF.

Lipid Profile as an Indicator of Severity in Cirrhosis of Liver: Hospital based Cross-sectional Study.

Various scoring systems are available to assess the severity of cirrhosis, that is, the Child-Pugh score and Model for End-Stage Liver Disease (MELD) score. Since the liver is the major site for converting excess carbohydrates into various lipids, the deranged lipid profile can act as a prognostic biomarker of cirrhosis. We assessed the lipid profile abnormalities among patients with cirrhosis of the liver and correlated them with the severity of cirrhosisMaterials and methods: This is an analytical cross-sectional study on lipid profile as an indicator of severity in cirrhosis of the liver among patients admitted to the medical ward of a tertiary care teaching hospital in Tamil Nadu. Following detailed investigation and confirmation of cirrhosis, a fasting serum lipid profile was measured in all eligible patients with cirrhosis. Total serum cholesterol, triglyceride (TGL), and high-density lipoprotein (HDL) were measured by direct method and serum low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) were calculated by using the Friedwald formulaResults: A total of 120 patients were studied. Of them, 76 (63%) were male. Of them, alcohol (84, 75.0%), hepatitis B (8, 7.1%), and nonalcoholic steatohepatitis (NASH) (6, 5.4%) were the most common cause of cirrhosis. A clear dose-response relationship (decreasing trend) is seen in the levels of lipids for increasing severity based on the Child-Pugh score and MELD score (except for a score of ≤10). Further, the cholesterol, LDL, and HDL were significantly lower among patients with ascites or with spontaneous bacterial peritonitis compared to their respective groups. However, none of the lipid profiles significantly differed based on the presence of upper gastrointestinal (UGI) bleeding Conclusion: This study observed that there is a significant reduction in levels of lipid profile parameters like serum total cholesterol, LDL, VLDL, TGL, and HDL in patients with cirrhosis as the severity increases. Further formulation of the scoring system in association with a preexisting scoring system may provide a better assessment of patients' prognosis in view of morbidity and mortality. We recommend it is necessary to assess the fasting lipid profile in all patients with cirrhosis and prognosticate their disease progression.

Dietary Supplementation with Resveratrol Attenuates Serum Melatonin Level, Pro-Inflammatory Response and Metabolic Disorder in Rats Fed High-Fructose High-Lipid Diet under Round-the-Clock Lighting.

This study aims to investigate the effect of resveratrol on systemic inflammatory response and metabolic disorder in rats fed a high-fructose high-lipid diet (HFHLD) and exposed to round-the-clock lighting (RCL). 21 adult male Wistar rats were randomly divided into 3 groups: control (group 1, n = 7); HFHLD for 8 weeks + round-the-clock lighting (RCL) (group 2, n = 7); HFHLD + RCL + Resveratrol (in a daily dose of 5 mg/kg intragastrically (group 3, n = 7). Results show that the combined effect of HFHLD and RCL reduces the serum melatonin (p < 0.001) and accelerates pro-inflammatory activities, oxidative stress, and metabolic disorder. There is a significant increase in the serum tumour necrosis factor-alpha (TNF-α) and C-reactive protein (CRP) (both p < 0.001), blood malondialdehyde-thiobarbituric acid adducts (MDA-TBA2) (p < 0.001), serum glucose (p < 0.01), insulin concentration, and the homeostatic model assessment insulin resistance (HOMA-IR) index (both p < 0.001), serum with very low-density lipoprotein (VLDL), and triacylglycerol (TAG) (both p < 0.001). At the same time, the decrease in the serum high-density lipoprotein (HDL) level (p < 0.001) is observed in the HFHLD + RCL group compared to the control. In the HFHLD + RCL + Resveratrol group, hypomelatonaemia (p < 0.001), pro-inflammatory actions, oxidative stress, and metabolic disorder were mitigated. Resveratrol can cause a significant rise in the serum melatonin and reduce serum TNF-α and CRP levels (both p < 0.001), blood MDA-TBA2 (p < 0.001), serum glucose (both p < 0.01), insulin concentration, and HOMA-IR (both p < 0.001), serum VLDL and TAG (both p < 0.001) compared to the group 2, while serum HDL level increases (p < 0.01). Resveratrol attenuates pro-inflammatory responses and prevents considerable metabolic disorder in rats fed HFHLD under RCL.

A STUDY OF THYROID PROFILE AND FASTING LIPID PROFILE IN CHRONIC KIDNEY DISEASE

Background: Chronic kidney disease [CKD] is associated with specific abnormalities in the thyroid and lipoprotein metabolism both in the early and in the advanced stages of chronic renal failure. Regardless of age, heart disease is a major cause of morbidity and mortality among patients with renal failure.Our study aimed at to estimate thyroid profile and dyslipidemia in CKD patients on conservative management,on haemodialysisand to compare with healthy controls. Methods: This was a prospective study conducted among the 100 CKD patients and controls over 18 months admitted in the department of general medicine at our hospital. Results: Hundred CKD cases with 70 male and 30 female in a M: F ratio of 2.3:1 were found to be in different stages CKD (12, 29 and 59 in stage-3 to stage-5 respectively). In 59 cases of stage-5 CKD, 32 were on HD and 27 on conservative management. In each grade of CKD, the mean age, eGFR, urea, creatinine, thyroid profile, and lipid profile were computed individually. The levels of urea, creatinine, and eGFR differed significantly across CKD grades 3-5.The thyroid profile differed significantly across CKD grades 3-5. The lipid profile differed significantly across CKD grades 3-5, with p=0.000, &gt;0.05, 0.000, &gt;0.05, &gt;0.05 for total cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) levels, respectively. Conclusions: The number of patients increases with decreasing T3 and T4 and increasing TSH proportionate to the severity of the renal failure, hypothyroidism is becoming more common in people with chronic renal disease.All lipid abnormalities found in CKD on HD have reduced HDL levels in serum along with significant rise in serum triglyceride, serum cholesterol serum LDL level and serum VLDL level.Dyslipidemia in CKD worsened as patientsprogresssed to severe stages with significant increase in TG, TC, VLDLc and TC/HDLc confirming presence of atherogenic lipid profile needing early intervention to prevent cardiovascular complications.

Atorvastatin remodels lipid distribution between liver and adipose tissues through blocking lipoprotein efflux in fish.

The regulation of cholesterol metabolism in fish is still unclear. Statins play important roles in promoting cholesterol metabolism development in mammals. However, studies on the role of statins in cholesterol metabolism in fish are currently limited. The present study evaluated the effects of statins on cholesterol metabolism in fish. Nile tilapia (Oreochromis niloticus) were fed on control diets supplemented with three atorvastatin levels (0, 12, and 24 mg/kg diet, ATV0, ATV12, and ATV24, respectively) for 4 wk. Intriguingly, the results showed that both atorvastatin treatments increased hepatic cholesterol and triglyceride contents mainly through inhibiting bile acid synthesis and efflux, and compensatorily enhancing cholesterol synthesis in fish liver (P < 0.05). Moreover, atorvastatin treatment significantly inhibited hepatic very-low-density lipoprotein (VLDL) assembly and thus decreased serum VLDL content (P < 0.05). However, fish treated with atorvastatin significantly reduced cholesterol and triglycerides contents in adipose tissue (P < 0.05). Further molecular analysis showed that atorvastatin treatment promoted cholesterol synthesis and lipogenesis pathways, but inhibited lipid catabolism and low-density lipoprotein (LDL) uptake in the adipose tissue of fish (P < 0.05). In general, atorvastatin induced the remodeling of lipid distribution between liver and adipose tissues through blocking VLDL efflux from the liver to adipose tissue of fish. Our results provide a novel regulatory pattern of cholesterol metabolism response caused by atorvastatin in fish, which is distinct from mammals: cholesterol inhibition by atorvastatin activates hepatic cholesterol synthesis and inhibits its efflux to maintain cholesterol homeostasis, consequently reduces cholesterol storage in fish adipose tissue.

Thyroid and lipid profile in chronic kidney disease in Southern India

Background: The progression of chronic kidney disease (CKD) is linked to a multitude of comorbidities, such as thyroid dysfunction, dyslipidemia, and cardiovascular disease. Objective were to determine the thyroid and serum lipid profile of CKD patients and to establish correlation between severity of renal disease with these 2 metabolic parameters.Methods: This was a prospective study conducted among the 100 CKD patients over 1 year admitted in the department of urology and nephrology at our hospital.Results: There were 66 (66%) male patients and 36 (36%) female patients among the 100 patients. There were no patients in grade 1, whereas there were 2, 20, 66, and 12 patients in grades 2-5 CKD, respectively. In each grade of CKD, the mean age, eGFR, urea, creatinine, thyroid profile, and lipid profile were computed individually. The levels of urea, creatinine, and eGFR differed significantly across CKD grades 2-5. The thyroid profile differed significantly across CKD grades 2-5. The lipid profile differed significantly across CKD grades 2-5, with p=0.000, &gt;0.05, 0.000, &gt;0.05, &gt;0.05 for total cholesterol, triglycerides, high-density lipoprotein (HDL), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) levels, respectively.Conclusions: The number of patients increases with decreasing T3 and T4 and increasing thyroid stimulating hormone (TSH) proportionate to the severity of the renal failure. In addition, hypothyroidism is becoming more common in people with chronic renal disease. Serum triglycerides, LDL, and VLDL levels rise statistically significantly in CKD grades 3-5 patients.

Comprassion the Lipid Profile among Children Suffreing from Nephrotic Syndrome before and after Remission

Aims: The study aimed to investigate serum cholesterol, triglycerides, LDL (low density lipoprotein), VLDL (very low-density lipoprotein), and HDL (high density lipoprotein) levels in nephrotic syndrome at the onset and during remission in first episode and relapse cases, as well as the relationship between dyslipidemia persistence and severity and disease duration and relapse frequency.&#x0D; Materials and Methods: A hospital-based prospective study including 30 children aged 0 to 12 years with nephrotic syndrome. They were steroid responsive in 22 cases and steroid dependent in 8 cases. They were assessed clinically and a lipid profile was taken at the start, during remission, and after treatment. A total of 30 children without liver or kidney disease were included as controls.&#x0D; Results: The mean blood cholesterol, triglycerides, LDL, and VLDL all increased significantly (p0.005). When compared to controls, HDL levels increased dramatically (P value 0.001) after nephrotic syndrome treatment. Lipid levels (serum cholesterol, triglycerides, LDL, VLDL) were significantly lower during remission in first-episode nephrotic syndrome cases, whereas lipid levels were significantly greater even during remission in recurrent cases. After treatment, total cholesterol and TGL levels were found to be higher, with P values of 0.004 and 0.004 respectively, as the duration of disease increased.&#x0D; Conclusion: The current investigation demonstrates that widespread hyperlipidemia is present in nephrotic syndrome. When compared to recently diagnosed with NS, this was much higher in relapse cases. Lipid profiles return to normal during remission recently diagnosed with NS, but they are considerably higher in recurrence instances, even during remission. As a result, there is a justification for treatment.

Is Coriandrum sativum Hypolipidemic in Alloxan Induced Diabetic Rats?

Introduction: Diabetes mellitus is a widely recognized metabolic condition. Due to various side effects of currently available medicines, studies have reported the use of many plants in various areas for the traditional management of diabetes.&#x0D; Aims &amp; Objectives: To determine the effects of Coriandum sativum seeds on lipid profile in diabetic rats.&#x0D; Place and duration of study: This experimental research was conducted at Physiology Department, Services Institute of Medical Sciences (SIMS), Lahore from August 2013 to January, 2014.&#x0D; Material &amp; Methods: Ninety rats were randomly distributed into three groups of (n=30) each. Group A was healthy control, group B was disease control and group C was diabetic experimental which was treated with Coriandrum sativum with a single dosage of 250 mg/kg of body weight/day through a gavage tube for 28 days. On next day, 4-5ml blood was collected through heart from each rat. The markers evaluated were high density lipoprotein (HDL), triglycerides, total cholesterol, low density lipoprotein (LDL) and very low density lipoprotein (VLDL). Data was analyzed by using SPSS version16 , p value &lt;0.05 was considered significant.&#x0D; Results: The experimental group showed highly significant (p=0.000) lower levels of serum LDL (25.47±3.90mg/dl), serum cholesterol (100.33±2.81 mg/dl), serum triglyceride (96.97±4.79 mg/dl) and serum VLDL (19.40±0.97 mg/dl) as compared to healthy control and disease control group. The level of serum high density lipoprotein revealed (55.27±3.69 mg/dl) highly significant rise (p=0.000).&#x0D; Conclusion: Oral administration of Coriandrum sativum has revealed the lipid lowering impacts in alloxan induced diabetic rats.

Immunometabolic response in Egyptian water buffalo cows during the transition period.

Background and Aim:The transition period is extremely critical for pregnant producing animals. However, there is very limited research on the metabolic and immunological changes in Egyptian water buffalo cows during the transition period. Therefore, this study was conducted to investigate the immunometabolic changes occurring during the transition period in Egyptian water buffalo cows.Materials and Methods:A total of 50 multiparous pregnant Egyptian water buffalo cows were subjected to weekly blood sampling 3 weeks before calving and 3 weeks after calving and on the day of parturition to determine the complete blood count, including red blood cell count, total leukocyte count (TLC), differential leukocyte count, hemoglobin level, and packed cell volume (PCV). Some selected serum biochemical and immunological parameters were analyzed, including serum glucose, beta-hydroxybutyric acid (BHBA), non-esterified fatty acids, triglycerides, high-density lipoprotein, low-density lipoprotein (LDL), very LDL (VLDL), cholesterol, total protein, albumin, globulin, creatinine, blood urea nitrogen (BUN), aspartate aminotransferase, alkaline phosphatase, alanine transaminase, gamma-glutamyl transferase, Haptoglobin, and C-reactive protein and the pro-inflammatory cytokines interleukin β1, interleukin 6 (IL-6), and tumor necrosis factor-alpha. All data were statistically analyzed using the IBM Statistical Package for the Social Sciences statistics software.Results:The neutrophil count showed a statistically significant increase at 2 weeks preparturition. There was also a significant increase in PCV, TLC, neutrophil count, and IL-6 and TNF-a level at the time of parturition and even at 2 weeks post parturition, except PCV that returned to normal levels in the 1st week post parturition. BHBA and BUN levels were increased significantly in the 2nd and 3rd weeks postcalving. Serum creatinine and VLDL levels were decreased significantly at the time of parturition, and VLDL levels showed a significant decrease even till the 3rd week postcalving, whereas creatinine levels gradually returned to the pre-calving levels in the 3rd week postcalving. Other parameters showed no significant changes.Conclusion:The most important immunometabolic changes occur in the first 2 weeks post parturition in Egyptian water buffalo cows, which exhibit a potent, remarkable physiological adaptation achieved by their functional liver, which can help the animal overcome the stressful conditions during the transition period.

Pemafibrate decreases triglycerides and small, dense LDL, but increases LDL-C depending on baseline triglycerides and LDL-C in type 2 diabetes patients with hypertriglyceridemia: an observational study

BackgroundPemafibrate, a selective PPARα modulator, has the beneficial effects on serum triglycerides (TGs) and very low density lipoprotein (VLDL), especially in patients with diabetes mellitus or metabolic syndrome. However, its effect on the low density lipoprotein cholesterol (LDL-C) levels is still undefined. LDL-C increased in some cases together with a decrease in TGs, and the profile of lipids, especially LDL-C, during pemafibrate administration was evaluated.MethodsPemafibrate was administered to type 2 diabetes patients with hypertriglyceridemia. Fifty-one type 2 diabetes patients (mean age 62 ± 13 years) with a high rate of hypertension and no renal insufficiency were analyzed. Pemafibrate 0.2 mg (0.1 mg twice daily) was administered, and serum lipids were monitored every 4–8 weeks from 8 weeks before administration to 24 weeks after administration. LDL-C was measured by the direct method. Lipoprotein fractions were measured by electrophoresis (polyacrylamide gel, PAG), and LDL-migration index (LDL-MI) was calculated to estimate small, dense LDL.ResultsPemafibrate reduced serum TGs, midband and VLDL fractions by PAG. Pemafibrate increased LDL-C levels from baseline by 5.3% (− 3.8–19.1, IQR). Patients were divided into 2 groups: LDL-C increase of > 5.3% (group I, n = 25) and < 5.3% (group NI, n = 26) after pemafibrate. Compared to group NI, group I had lower LDL-C (2.53 [1.96–3.26] vs. 3.36 [3.05–3.72] mmol/L, P = 0.0009), higher TGs (3.71 [2.62–6.69] vs. 3.25 [2.64–3.80] mmol/L), lower LDL by PAG (34.2 [14.5, SD] vs. 46.4% [6.5], P = 0.0011), higher VLDL by PAG (28.2 [10.8] vs. 22.0% [5.2], P = 0.0234), and higher LDL-MI (0.421 [0.391–0.450] vs. 0.354 [0.341–0.396], P < 0.0001) at baseline. Pemafibrate decreased LDL-MI in group I, and the differences between the groups disappeared. These results showed contradictory effects of pemafibrate on LDL-C levels, and these effects were dependent on the baseline levels of LDL-C and TGs.ConclusionsPemafibrate significantly reduced TGs, VLDL, midband, and small, dense LDL, but increased LDL-C in diabetes patients with higher baseline TGs and lower baseline LDL-C. Even if pre-dose LDL-C remains in the normal range, pemafibrate improves LDL composition and may reduce cardiovascular disease risk.

Effect of vegetable oils supplementation on serum biochemical profile of lactating surti goats and their kids

A study was conducted to observe the effect of soybean and rice bran oil supplementation on serum biochemical profile of Surti goats and their nursing kids during December 2016 to April 2017. Multiparous lactating Surti does (n=24) were distributed into four groups in completely randomized design for entire lactation period of 150 days. The goats of control (CON) group were offered a basal diet consisting of concentrate, chaffed green and dry fodder. The goats of treatment groups viz., SBO (soybean oil @ 3% of DMI (dry matter intake), RBO (rice bran oil @ 3% of DMI) and SRBO (soybean oil @ 1.5% + rice bran oil @ 1.5% of DMI) were supplemented with different vegetable oils along with basal diet. Kids from does of respective treatment groups were also assigned the same group for evaluating the growth performance. Blood sample were collected on 0, 30, 60, 90,120 and 150 days from lactating does and on 45 and 50 days of age in kids. Serum glucose, total protein, triglycerides, LDL (low-density lipoprotein) and VLDL (very low-density lipoprotein) cholesterol and urea nitrogen were neither affected by diet nor time in does of different treatment groups and their respective nursing kids. However, total and HDL (High-density lipoprotein) cholesterol were significantly (P<0.05) improved in lactating does supplemented with oils than the control. Further, maternal dietary lipid sources modified and improved circulatory lipid profile in suckling kids. However, average daily gain in nursing kids remained non-significant among the treatment groups. It was concluded that dietary supplementation of vegetable oil either alone or in combination can be effectively used to improvecirculatory lipidprofile of does andtheirnursing kids.

Association of serum VLDL level with hyperhomocysteinemia in hypertensive patients: A cross-sectional study

ABSTRACT Background Increasing evidence suggests that hyperhomocysteinemia (HHcy) and hyperlipidemia have been recognized as two independent risks for cardiovascular disease. However, the association between hyperlipidemia and HHcy in hypertensive patients has not been systemically elucidated. The aim of this study was to investigate the relation between very low-density lipoprotein (VLDL) and HHcy in hypertensive patients. Methods From July 2013 to March 2014, a large cross-sectional study was performed using 4012 participants from urban and rural communities in Hunan province, China. Participants underwent accurate assessment of lipid profiles, homocysteine (Hcy), anthropometric, blood pressure, and other biochemical indicators. Results Among 1257 participants with hypertension, 626 (49.80%) were men and 631 (50.20%) were women. In total, 1081 (86.00%) of the participants were found to have HHcy, of which 559 (44.47%) were men and 522 (41.53%) were women. In the univariate analysis, the OR for patients with hypertension associated with hyperhomocysteinemia was significantly enhanced as the quartiles of the Log VLDL were increased. OR for quartile 4 was significantly higher than that for quartile 1 (OR = 3.7, 95% CI: 2.6–5.1; P< .001). Additional adjustment for the confounding variables did not reduce the ORs for the association between the Log VLDL and hypertension associated with hyperhomocysteinemia (OR = 3.8, 95% CI: 2.7–5.5; P< .001; OR = 4.3, 95% CI: 1.6–11.8; P= .004, respectively). We also conducted analyses with Log VLDL as a continuous variable. Each unit increase in the Log VLDL was associated with the 1.3-fold increased risk of hypertension associated with hyperhomocysteinemia (95% CI: 1.9–2.9; P< .001). Adjusting for Cr, TG, TC, and HDL did not affect the relationship. Conclusions Our data indicate that the Log VLDL concentrations appear to be an independent contributor to hypertension associated with hyperhomocysteinemia, even after adjusting for age and other covariables. The utility of the Log VLDL as a diagnostic, prognostic, and therapeutic indicator for the disease warrants further investigation. Abbreviations HHcy: hyperhomocysteinemia; Hcy: homocysteine; VLDL: very low-density lipoprotein; CVD: cardiovascular disease; SBP: systolic blood pressure; DBP: diastolic blood pressure; BMI: body mass index; ALT: alanine aminotransferase; Cr: creatinine; UA: uric acid; TG: triglycerides; TC: total cholesterol; HDL: high-density lipoprotein; LDL: low-density lipoprotein; FBG: fasting blood glucose; CRP: C-reactive protein; MTHFR: methylene tetrahydrofolate reductase; NO: nitric oxide; HDL-C: high-density lipoprotein cholesterol.

Maternal hypercholesterolemia programs dyslipidemia in adult male mouse progeny.

As a collection of metabolic abnormalities including inflammation, insulin resistance, hypertension, hormone imbalance, and dyslipidemia, maternal obesity has been well-documented to program disease risk in adult offspring. Although hypercholesterolemia is strongly associated with obesity, less work has examined the programming influence of maternal hypercholesterolemia (MHC) independent of maternal obesity or high-fat feeding. This study was conducted to characterize how MHC per se impacts lipid metabolism in offspring. Female (n = 6/group) C57BL/6J mice were randomly assigned to: (1.) a standard chow diet (Control, CON) or (2.) the CON diet supplemented with exogenous cholesterol (CH) (0.15%, w/w) throughout mating and the gestation and lactation periods. At weaning (postnatal day (PND) 21) and adulthood (PND 84), male offspring were characterized for blood lipid and lipoprotein profile and hepatic lipid endpoints, namely cholesterol and triglyceride (TG) accumulation, fatty acid profile, TG production, and mRNA expression of lipid-regulatory genes. Both newly weaned and adult offspring from CH mothers demonstrated increased very low-density lipoprotein (VLDL) particle number and size and hepatic TG and n-6 polyunsaturated fatty acid accumulation. Further, adult CH offspring exhibited reduced fatty acid synthase (Fasn) and increased diglyceride acyltransferase (Dgat1) mRNA expression. These programming effects appear to be independent of changes in hepatic TG production and postprandial lipid clearance. Study results suggest that MHC, independent of obesity or high-fat feeding, can induce early changes to serum VLDL distribution and hepatic lipid profile that persist into adulthood.

Serum Lipids among Drug Naïve or Drug-Free Patients with Obsessive Compulsive Disorder and their Association with Impulsivity: A Comparative Study.

Background:The derangement of serum lipids is well documented in psychiatric disorders like schizophrenia, mania, and depression but not in obsessive compulsive disorder (OCD), where it has been inadequately examined. Also, serum lipid abnormalities are increasingly found in “impulsivity,” an important sub-construct of OCD. Our study aimed to examine serum lipid profile among patients with OCD and its association with clinical profile and impulsivity among them.Methods:Forty drug naïve or drug-free (four weeks for oral and eight weeks for any depot psychotropics) patients with OCD according to International Classification of Disease -10th version (ICD-10): Diagnostic Criteria for Research (DCR) by the World Health Organization (WHO), from outpatient and inpatient departments of a tertiary care psychiatric hospital were recruited. Measures like Yale–Brown Obsessive Compulsive Scale (Y-BOCS), Hamliton Rating Scale for Depression (HAM-D), Barratt's Impulsivity Scale (BIS-11), and Hamilton Rating Scale for Anxiety (HAM-A) were administered. Forty age and sex-matched healthy controls (HC) were recruited after screening with General Health Questionnaire 12 (GHQ-12). Serum lipids were assessed in both the groups.Results:Serum high density lipoproteins (HDL) (P < 0.001; partial η2 = 0.176) and apolipoprotein B (P < 0.001; partial η2 = 0.531) were significantly higher in OCD group than age- and sex-matched HC. A trend toward lower serum HDL (P = 0.06; partial η2 = 0.060) was observed among patients of OCD with high impulsivity. Serum HDL was negatively correlated with BIS attention (rs =-0.32; p = 0.03), BIS motor (rs = 0.40; P = 0.01), BIS non-planning (rs = - 0.36; P = 0.02), and BIS total (rs = - 0.36; P = 0.01) scores. Serum triglycerides (TG) (rs = 0.34; P = 0.03) and apolipoprotein B (rs = -0.32; P = 0.04) were negatively correlated with Y-BOCS compulsion score. Serum TG (rs = -0.45, P < 0.01) and serum very low density lipoprotein (VLDL) was negatively (rs = -0.39; P = 0.01) correlated with Y-BOCS total scores. Serum VLDL was positively (rs = 0.34; P = 0.03) correlated with BIS motor scores.Conclusions:Serum lipid fractions are deranged among patients with OCD. Different lipid fractions have different associations with clinical profiles of OCD. Impulsivity among patients with OCD may have a specific association with serum lipids. A small sample size, use of self-report measure without adaptation for impulsivity, a lack of metabolic profile assessment among participants, and a lack of assessment of impulsivity among HC were the limitations of our tudy.

Estimation of Serum Homeostasis Model Assessment-Insulin Resistance and Lipid Profile in Beta-thalassemia Major Patients and their Correlation with Iron Overload in Koya City

The current study focused on 43 patients who suffer from major beta-thalassemia at the hospital of shaheed Dr. Khaled in the Koya city. Out range, age of cases was 8.03 ± 4.0 and of controls was 7.81 ± 3.11 years. Our aim is to observe the prevalence of homeostasis model assessment-insulin resistance and other physiological and biochemical changes in major beta-thalassemia. While we a significant changes confirm that serum glucose concentration was significantly higher in the patients than in the controls (P ≤ 0.01) and lipid abnormality occurs in beta-thalassemia major patients, which include high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), serum total cholesterol (TC), serum triglycerides (TG), and very-low-density lipoprotein (VLDL) levels compared with normal healthy controls. There was no significant difference between the serum insulin level of cases and controls (P = 0.214), the mean values of serum HDL-C, LDL-C, TC, TG, and VLDL in cases were 29.5 ± 7.8, 65.2 ± 1.9, 121.9 ± 36.7, 182.09 ± 43.1, and 26.47 ± 12.13 mg/dl, respectively. Moreover, the mean values of serum HDL-C, LDL-C, TC, TG, and VLDL in controls were 48.6 ± 4.2, 79.7 ± 14.5, 178.7 ± 14.6, 124.14 ± 12.1, and 23.52 ± 5.47 mg/dl, respectively. In conclusion, the results suggested that revealed that beta-thalassemia patients had hypertriglyceridemia, hypocholesterolemia, and low LDL-C, and HDL-C levels.