Serum Trace Metal Research Articles

Analysis by pattern recognition techniques of changes in serum levels of 14 trace metals after acute myocardial infarction

Serial changes in the concentrations of 14 trace metals (Cu, Fe, Al, Ni, Sr, Ba, Mn, Cs, Sn, Cr, Zn, Pb, Mo, and Cd) in blood serum of patients who had sustained an acute myocardial infarction and in patients who had sustained other cardiac trauma were determined by emission spectrometry. Comparable data for both patient groups were obtained for the serum enzymes, creatine phosphokinase, glutamic oxaloacetic transaminase, and lactic dehydrogenase. Multivariate analysis of variance indicated that changes in serum levels of copper, iron, aluminum, and nickel during the first 7 days following an acute myocardial infarction might be useful in the classification of patients. Using such a trace-metal profile, the discriminant analysis technique of pattern recognition provided almost complete (91%) success in distinguishing the two patient groups. Complete discrimination was achieved when the covariates age, race, and sex were included pairwise with the four trace metals, Cu, Fe, Al, and Ni. The studies suggest that trace metals may play an important role in cardiovascular metabolism and the chemical pathology of myocardial infarction.

An endogenous mediator of depression of amino acids and trace metals in serum during typhoid fever.

Concentrations of most amino acids and of zinc in serum were depressed during periods of incubation and illness in 11 volunteers who developed symptoms after an oral dose of 105 virulent Salmonella typhosa. When a 1.0-ml sample of sterile serum from volunteers who were ill with typhoid fever was injected into normal rats, it stimulated a prompt and significant depression of the concentration of zinc in the rats' sera and a flux of amino acids into their livers. These observations support the hypothesis that an endogenous factor (similar to endogenous pyrogen released by polymorphonuclear leukocytes) was present in the blood during typhoid fever and served as a mediator for the observed depression in zinc and amino acids in serum. The magnitude and pattern of infection-related depression in individual amino acids in serum may be, in part, a function of the amount of endogenous mediator released and of the rates of utilization of amino acids by tissues.

Instrumental and radiochemical neutron activation analysis techniques for protein bound trace metals in human serum

Gel filtration and neutron activation analysis methods were combined in work aimed at the development of methods for the determination of metals firmly bound to protein in human blood serum. The values obtained are semiquantative. A purely instrumental technique afforded data on Cu, Fe, Zn, Al and Mn but values for the latter two only were determined since Cu, Fe and Zn are already well known. The concentrations for Al and Mn were found to be 300 ppb and 0.4 ppb, respectively. Despite the high resolution of Ge(Li) detectors, no other metals were found. For the determination of any others, radiochemical separations are necessary and were used to obtain data on the following elements: As 3 ppb, Ag 3 ppb, Au 0.003 ppb, Cr 1 ppb, Sb 1 ppb, Co, Cd, Mo and Sn could not be detected and upper limits were estimated to be 0.1 ppb, 2 ppb, 2 ppb, and 1 ppb, respectively.

Recorder curve integration for quantitating trace metals in serum by atomic absorption spectroscopy.

Journal Article Recorder Curve Integration for Quantitating Trace Metals in Serum by Atomic Absorption Spectroscopy Get access Denis O. Rodgerson, M.S., F.I.M.L.T., Denis O. Rodgerson, M.S., F.I.M.L.T. Pediatric Microchemistry Laboratory, Department of Pediatrics, University of Colorado Medical Center, 4200 East Ninth Avenue, Denver, Colorado 80220 Search for other works by this author on: Oxford Academic Google Scholar Ray E. Helfer, M.D. Ray E. Helfer, M.D. Pediatric Microchemistry Laboratory, Department of Pediatrics, University of Colorado Medical Center, 4200 East Ninth Avenue, Denver, Colorado 80220 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 46, Issue 1, 1 July 1966, Pages 63–64, https://doi.org/10.1093/ajcp/46.1.63 Published: 01 July 1966 Article history Received: 13 December 1965 Published: 01 July 1966