Serum Se Research Articles

Level of Serum Selenium in Bronchial Asthma Patients in a Tertiary Care Center: A Cross-sectional Study

Introduction: Selenium (Se) is a trace element that has role as an antioxidant in acute stress like; systemic inflammatory responses or trauma. Literature shows positive correlation between low serum Se levels and bronchial asthma. Aim: To determine the level of serum Selenium (Se) in bronchial asthma and to compare the Se levels with the age-matched control group without asthma. Materials and Methods: This cross-sectional study was conducted at the Departments of Physiology and Pulmonary Medicine of a Government Tertiary-care Hospital in Kerala, from November 2021 to March 2022. A total of 24 patients of Bronchial Asthma, between 20-55 years of age and 24 age-matched controls were selected. Serum Se levels were estimated in cases and controls, using Atomic Absorption Spectrometer (AAS). Statistical analysis of the data was done using unpaired sample t-test and correlation was tested with Chi-square test, using Statistical Package for Social Sciences (SPSS) version 27.0. Results: Serum Se levels of 24 patients with Bronchial asthma, aged between 25-55 years and the Se levels in the age-matched controls were obtained. The mean Se level in the study group was (67.21±39.57 ngm/L) significantly lower (p-value was 0.0001) than that of the control (108.38±19.22 ngm/L). The mean age was 39 years±10.8 years. Out of them, 40% were females and the remaining 60% were males. The severity of Asthma ranged between mild persistent asthma to moderate asthma. And the mean duration of asthma was 5.59 years. Conclusion: Serum Se in adult bronchial asthma patients aged between 20 years to 55 years, with mild to moderate severity was significantly lower than that in the control group

Comparative analysis of various sources of selenium on the growth performance and antioxidant status in broilers under heat stress.

The supplementation of Selenium-enriched probiotics is effective in reducing oxidative stress and maintaining meat quality stability in broiler chicken especially under heat stress. An experimental study was conducted to perform Comparative analysis of Selenium yeast with inorganic Se in broilers under heat stress. A total of 120 broilers chicks of one day were assigned to 4 groups each consisting 30 chicks fed on same basal diet but different selenium sources. The basal diet of group D1 was not supplemented with Se source (Negative control), group D2 basal diet was supplemented with inorganic selenium (Sodium selenite 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase), group D3 basal diet was supplemented with commercially available organic selenium (Seleno-methionine 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase) and group D4 basal diet was supplemented with self-developed organic selenium (Se-enriched yeast 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase). The performance parameters i.e. feed intake (FI), live body weight (BW) and FCR were not significantly (p>0.05) effected by selenium supplementation in the starter phase but were significantly (p<0.05) effected in the finisher phase. Selenium supplementation significantly (p<0.05) effected serum Se level in different supplemented groups. Higher serum Se value (58.20±0.06) was recorded in D4 group. Similarly significantly lower selenium value was recorded for D4 and higher was recorded for D1 (11.36±0.08). However lower serum Paraoxonase (PON) value was recorded for D4 (13.24±0.01) and higher for D1 (13.33±0.03). Comparatively self-developed Se enriched yeast increased the Se accumulation and improved antioxidant system. Glutathione peroxidase (GPx) was found higher in D4 (12.333±0.03) followed by D3, D2 and D1 respectively. Whereas superoxide dismutase (SOD) was significantly lower (p<0.05) in D4 (0.1437±0.003) followed by D3 (0.1457±0.002). Selenium supplementation increased the bird's survival rate. Birds fed on Se enriched yeast showed higher Se deposition and better antioxidant capacity as compared to other sources of selenium. Se-enriched yeast displayed an improved result on Se deposition in tissues, and oxidative capacity, meat tenderness and immune response level as compared to other sources of selenium.

A pilot study investigating the relationship between serum Se concentrations and Selenoprotein P activity at 28wks gestation in a high fish-eating sub-cohort of Seychellois pregnant women

A pilot study investigating the relationship between serum Se concentrations and Selenoprotein P activity at 28wks gestation in a high fish-eating sub-cohort of Seychellois pregnant women

Associations of trace element levels in paired serum, whole blood, and tissue: an example of esophageal squamous cell carcinoma.

Previous studies have extensively explored impacts of trace elements on human beings and complex relationships with cancers. However, contradictory conclusions may be more challenging to explain due to biological specimen differences. To investigate the distribution of trace elements inside body, we collected serum, whole blood and tissues from 77 patients with esophageal squamous cell carcinoma (ESCC), as well as serum and whole blood from 100 healthy individuals, and determined the concentrations of 13 elements (Al, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Sr, Cd, and Pb) with inductively coupled plasma-mass spectrometry (ICP-MS). Al, Ni, Cu, Sr, and Cd variations between patients and controls were found to be inconsistent in serum and blood. Concentrations of Cu, As, Se, and Sr in serum were positively correlated with that in whole blood in both case and control group (rs >0.450, P <0.01). Elements in serum had a higher accuracy (87.0%) than whole blood (74.0%) in classifying ESCC patients and healthy individuals with discriminant analysis. As, Cd, and Pb concentrations in cancerous tissues were positively correlated with those in normal epithelium (rs =0.397, 0.571, and 0.542, respectively), while Mn, Cu, and Se accumulated in malignant tissues, with V, Cr, Co, Ni, Sr, and Cd partitioning in normal epithelium (all P <0.05). Thus, certain elements in blood, such as Cu, As, Se, and Sr, were useful in assessing element exposure burdens and accumulation tendency of some elements (Mn, Cu and Se, etc.) was uncovered in tumors. Our investigation demonstrated the variations in trace element distribution for frequently used specimens and further evidence of etiological mechanism is necessary.

High throughput drug screening identifies resveratrol as suppressor of hepatic SELENOP expression

IntroductionSelenium (Se) is an essential trace element that exerts its effects mainly as the proteinogenic amino acid selenocysteine within a small set of selenoproteins. Among all family members, selenoprotein P (SELENOP) constitutes a particularly interesting protein as it serves as a biomarker and serum Se transporter from liver to privileged tissues. SELENOP expression is tightly regulated by dietary Se intake, inflammation, hypoxia and certain substances, but a systematic drug screening has hitherto not been performed. MethodsA compound library of 1861 FDA approved clinically relevant drugs was systematically screened for interfering effects on SELENOP expression in HepG2 cells using a validated ELISA method. Dilution experiments were conducted to characterize dose-responses. A most potent SELENOP inhibitor was further characterized by RNA-seq analysis to assess effect-associated biochemical pathways. ResultsApplying a 2-fold change threshold, 236 modulators of SELENOP expression were identified. All initial hits were replicated as biological triplicates and analyzed for effects on cell viability. A set of 38 drugs suppressed SELENOP expression more than three-fold, among which were cancer drugs, immunosuppressants, anti-infectious drugs, nutritional supplements and others. Considering a 90% cell viability threshold, resveratrol, vidofludimus, and antimony potassium-tartrate were the most potent substances with suppressive effects on extracellular SELENOP concentrations. Resveratrol suppressed SELENOP levels dose-dependently in a concentration range from 0.8 μM to 50.0 μM, without affecting cell viability, along with strong effects on key genes controlling metabolic pathways and vesicle trafficking. ConclusionThe results highlight an unexpected direct effect of the plant stilbenoid resveratrol, known for its antioxidative and health-promoting effects, on the central Se transport protein. The suppressive effects on SELENOP may increase liver Se levels and intracellular selenoprotein expression, thereby conferring additional protection to hepatocytes at the expense of systemic Se transport. Further physiological effects from this interaction require analyses in vivo and by clinical studies.

Investigation of Serum Trace Element Levels in Sheep in Diyarbakır Province and Districts

Background: In this study, the serum of sheep raised on pasture in the province and districts of Diyarbakır was tested for concentrations of the essential trace elements Cr, Cu, Se, Zn, Co, Mn, ultra-trace elements like Ni, V and Ag and non-essential toxic trace elements like Al, Pb, As, Cd and Ti. Methods: The animals used in the study consisted of 313 healthy sheep at the end of lactation obtained from different districts of Diyarbakır. The closed system method of wet decomposition was used to transfer serum trace elements to the soluble phase in blood samples. Serum trace elements were analyzed using an ICP-MS device. Result: This study contributed to obtaining normal levels of Al, Ag, Ni, Ti and V trace elements in ewes at the end of lactation. It was observed that there was no difference between all districts in terms of serum Al, Ni, Cu and Zn trace element levels. In the study, differences were found in the serum Cr, Se, As and Cd trace element levels of sheep between the districts; however, it was determined that no significant deficiency or excess occurred and these levels were within normal limits. The Co and Mn levels we obtained in Diyarbakýr are much lower than the average levels reported by the researchers. We can say that the addition of Co and Mn trace elements in appropriate proportions to the rations of animals can positively affect animal health and productivity.

Associations of serum zinc, copper, and selenium with sleep disorders in the American adults: Data from NHANES 2011–2016

BackgroundEven though various studies have been conducted to investigate the relationship between trace metals and sleep, few epidemiological studies have evaluated the relationship between trace metals and sleep disorders in American adults. ObjectiveThis study intended to evaluate the associations of serum zinc (Zn), copper (Cu), selenium (Se), Zn/Cu, Zn/Se, and Cu/Se ratios with sleep disorders in American adults. MethodsWe conducted a cross-sectional analysis of 3660 adults aged ≥18 years old who participated in the National Health and Nutrition Examination Survey (NHANES) 2011–2016. Binary logistic regression was employed to calculate the odds ratio (OR) and 95 % confidence interval (CI) of either serum trace metals or serum trace metals ratios with risks among sleep disorder phenotypes. The restricted cubic spline (RCS) model was additionally utilized to check the dose-response relationships between serum trace metals, serum trace metals ratios, and sleep disorders. ResultsLogistic regression demonstrated that higher serum Zn (OR: 0.70, 95 % CI: 0.51–0.97, p = 0.035), Zn/Cu (OR: 0.62, 95 % CI: 0.45–0.87, p = 0.007), and Zn/Se (OR: 0.68, 95 % CI: 0.49–0.95, p = 0.025) were related to a decreased likelihood of self-reported sleep disorders, and dose-response relationships were detected by the RCS models, after adjustment for sociodemographic, behavioral, and health characteristics. No associations between serum Cu, Se, Cu/Se, and sleep disorders were observed. The findings in the sensitivity analyses were consistent with these results. ConclusionOur study revealed that serum Zn, Zn/Cu, and Zn/Se were inversely associated with the risk of self-reported sleep disorders in US adults.

Neutrophil function and antibody production during the transition period: Effect of form of supplementary trace minerals and associations with postpartum clinical disease and blood metabolites.

Our objectives were to evaluate the effect of the form of supplementary trace minerals-inorganic salts (STM: Co, Cu, Mn, and Zn sulfates and Na selenite) or organic (OTM: Co, Cu, Mn, Zn proteinates, and selenized yeast)-fed at 100% of recommended levels in both pre- and postpartum diets on in vitro phagocytic activity of neutrophils, and in vivo IgG responses to an ovalbumin challenge during the transition period. In addition, we investigated the associations of these immunological responses with incidence of postpartum clinical diseases and the dynamic changes of metabolic markers during the transition period. Pregnant heifers and cows (n = 273) were enrolled at 45 ± 3 d before expected calving, blocked by parity and body condition score, and allocated randomly to STM or OTM supplementation. Cows in both treatments were fed the same diet, except for the form of supplementary trace minerals. Automatic feeding gates were used to assign treatments to individual cows. Blood was collected on d -7 ± 3 and 7 ± 3 relative to calving in a subgroup of cows (n = 131 and 133, respectively) to measure phagocytic activity of neutrophils in vitro using flow cytometry. Subcutaneous immunization with 0.5 mg of chicken egg ovalbumin was performed in a subgroup of cows (n = 181) on d -45, -21, and 3 relative to calving. Concentration of anti-ovalbumin IgG in serum was measured by ELISA on d -45, -21, 3, 7, and 21. Trace mineral concentrations in blood were measured by inductively coupled plasma mass spectrometry on d -45, -21, -7, 0, 7, and 21 relative to calving. Selected metabolites were measured on d -21, -10, -3, 0, 3, 7, 10, 14, and 21 relative to calving. Treatment did not affect the percentage of neutrophils performing phagocytosis on d -7 or 7 but the median fluorescence intensity of phagocytosis on d 7 was greater for OTM than STM. We found no differences between treatments in the level of anti-ovalbumin IgG in serum on any of the sampling days. Changes in neutrophil function from prepartum to postpartum were associated with incidence of postpartum clinical disease, postpartum feed intake and milk production, concentrations of Ca, K, Se, Mn, Co, and total protein in serum. Immunoglobulin G responses to ovalbumin injections were not associated with incidence of postpartum clinical disease but were associated with body weight, feed intake, energy balance, and concentrations of nonesterified fatty acids, aspartate aminotransferase, gamma-glutamyl transpeptidase, albumin, Na, P, and Cu in serum. In conclusion, replacement of STM by OTM improved one measure of phagocytic capacity of neutrophils in vitro, which was also greater in cows that did not develop postpartum clinical disease. The associations of innate and acquired immune responses with feed intake, energy balance, and circulating concentrations of key macro and micronutrients reinforce the importance of nutritional management for the health of dairy cows during the transition period.

The Effect of Chitosan and Chitosan Oligosaccharide on Serum Mineral and Vitamin Levels in Rats with Experimental Fluorosis

Fluorine toxicity occurs in different parts of the world. It is caused by the increase in the fluorine level in the waters, especially due to the dissolution of volcanic rocks over time. Water with high fluorine content can be taken with plant and animal irrigations, and foods obtained from plants and animals, or it can be taken directly with drinking water. In this study, the effects of chitosan (CS) and chitosan oligosaccharide (COS) on some serum trace minerals and vitamin levels in experimental fluorosis rats were investigated. Six groups were formed as control, fluorosis, fluorosis CS and COS groups, healthy CS and COS groups. CS and COS were administered orally for 28 days at a dose of 250 mg/kg. At the end of the study, ketamine anesthesia was administered and the heart was directly cannulated. Trace minerals (Cu, Zn, Mn, Fe, Se) and vitamins (retinol, α-tocopherol, D3) were analyzed. Cu, Zn, Se and Mn levels were found to be decreased in the F group compared to the control group. A significant increase was found in the Zn level in the F+CS group and in the Cu, Zn and Se levels in the F+CS group compared to the values in the F group. Serum retinol and α -tocopherol levels were decreased in the F, F+CS, F+COS and CS groups. In conclusion, our findings showed that there was a decrease in serum Zn, Cu, Se, retinol and α-tocopherol levels in rats with fluorosis, and COS was more effective than CS against the decrease in mineral and vitamin levels.

Association of COVID-19 mortality with serum selenium, zinc and copper: Six observational studies across Europe.

Certain trace elements are essential for life and affect immune system function, and their intake varies by region and population. Alterations in serum Se, Zn and Cu have been associated with COVID-19 mortality risk. We tested the hypothesis that a disease-specific decline occurs and correlates with mortality risk in different countries in Europe. Serum samples from 551 COVID-19 patients (including 87 non-survivors) who had participated in observational studies in Europe (Belgium, France, Germany, Ireland, Italy, and Poland) were analyzed for trace elements by total reflection X-ray fluorescence. A subset (n=2069) of the European EPIC study served as reference. Analyses were performed blinded to clinical data in one analytical laboratory. Median levels of Se and Zn were lower than in EPIC, except for Zn in Italy. Non-survivors consistently had lower Se and Zn concentrations than survivors and displayed an elevated Cu/Zn ratio. Restricted cubic spline regression models revealed an inverse nonlinear association between Se or Zn and death, and a positive association between Cu/Zn ratio and death. With respect to patient age and sex, Se showed the highest predictive value for death (AUC=0.816), compared with Zn (0.782) or Cu (0.769). The data support the potential relevance of a decrease in serum Se and Zn for survival in COVID-19 across Europe. The observational study design cannot account for residual confounding and reverse causation, but supports the need for intervention trials in COVID-19 patients with severe Se and Zn deficiency to test the potential benefit of correcting their deficits for survival and convalescence.

Mercury, silver and selenium in serum before and after removal of amalgam restorations: results from a prospective cohort study in Norway

Objective A prospective cohort study on changes of health complaints after removal of amalgam restorations was carried out at the request of the Norwegian Directorate of Health. The aim was to provide and evaluate experimental treatment to patients with health complaints attributed to dental amalgam fillings. Methods Patients (n = 32) with medically unexplained physical symptoms (MUPS), which were attributed to dental amalgam restorations had all their amalgam restorations removed and replaced with other dental restorative materials. Samples of blood were collected before and 1 year after removal of the fillings, and concentration of inorganic mercury (I-Hg), methylmercury (MeHg), silver (Ag) and selenium (Se) in serum was determined by inductively coupled plasma–sector field mass spectrometry. The comparison groups (one with MUPS but without attribution to amalgam [n = 28] and one group of healthy individuals [n = 19]) received no treatment. The participants responded to questionnaires at baseline and at follow-up after 1 and 5 years. Results Concentration of I-Hg and Ag in serum decreased significantly after removal of all amalgam restorations. Concentration of MeHg and Se in serum were not changed. Intensity of health complaints was significantly reduced after amalgam removal, but there were no statistically significant correlations between exposure indicators and health complaints. Conclusions Removal of all amalgam restorations is followed by a decrease of concentration of I-Hg and Ag in serum. The results support the hypothesis that exposure to amalgam fillings causes an increase of the daily dose of both I-Hg and Ag. Even though intensity of health complaints decreased after removal of all amalgam restorations there was no clear evidence of a direct relationship between exposure and health complaints. Trial registration: The project is registered at Clinicaltrials.gov (NCT01682278).

Correlations of selenium and selenoprotein P with asymmetric dimethylarginine and lipid profile in patients with polycystic ovary syndrome

BackgroundPolycystic ovary syndrome (PCOS) is associated with an increased risk of cardiovascular diseases (CVD). Accumulating evidence has suggested that selenium (Se) is of importance for optimal function of the cardiovascular system. This study aimed to investigate the associations of selenium and selenoprotein P (SePP) with asymmetric dimethylarginine (ADMA) and lipid profile in women with PCOS. MethodsIn this cross-sectional study, 125 females aged 18–45 years diagnosed with PCOS were recruited. An interviewer-administered questionnaire was applied to gather the relevant demographic characteristics, detailed clinical information, and lifestyle habits of participants. Fasting blood samples were obtained to measure biochemical parameters. Serum concentrations of total testosterone, sex hormone-binding globulin (SHBG), ADMA, and lipid profiles as well as anthropometric measurements were assessed across tertiles of serum Se and SePP concentrations. ResultsThere was a positive correlation between serum Se and SePP concentrations (r = 0.434, p < 0.001). Serum Se level was inversely correlated with ADMA (r = −0.21, p = 0.025) and TG (r = −0.17, p = 0.041) concentrations. There were also inverse correlations between SePP and ADMA (r = −0.34, p < 0.001), TG (r = −0.21, p = 0.019), and oxidized low density lipoprotein (ox-LDL) (r = −0.25, p = 0.007) levels. No significant relationship was found between serum Se and SePP concentrations with total cholesterol, low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein-A1 (Apo-A1), apolipoprotein-B (Apo-B100), total testosterone, SHBG, and free androgen index as well as anthropometric parameters (All p > 0.05). ConclusionThe present study found that Se and SePP levels were inversely correlated with ADMA and TG concentrations as well as ox-LDL levels.

Evaluation among trace elements, clinical parameters and type 1 diabetes according to sex: A new sight of auxiliary prediction in negative insulin auto-antibodies population

BackgroundType 1 diabetes (T1D) exhibited sex-specific metabolic status including oxidative stress with dynamic change of trace elements, which emphasized the importance of the evaluation of trace elements according to sex. Besides, the most significant characteristic, insulin auto-antibodies, could not be found in all T1D patients, which needed the auxiliary prediction of clinical parameters. And it would benefit the early detection and treatment if some high-risk groups of T1D could predict and prevent the occurrence of disease through common clinical parameters. Hence, there was an urgent need to construct more effective and scientific statistical prediction models to serve clinic better. This study aimed to evaluate the sex-specific levels of trace elements and the relationship between trace elements and clinical parameters in T1D, and construct sex-specific auxiliary prediction model combined with trace elements and clinical parameters. MethodsA total of 105 T1D patients with negative insulin auto-antibodies and 105 age/sex-matched healthy individuals were enrolled in First Hospital of Jilin University. Inductively Coupled Plasma Mass Spectrometry was performed for the measurement of calcium (Ca), magnesium (Mg), zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in the serum, and the data of clinical parameters were received from medical record system. The lambda-mu-sigma method was used to evaluate the relationship between abnormal clinical parameters and trace elements. Training set and validation set were divided for the construction of predictable models in males and females: clinical parameters model, trace element model and the combined model (clinical parameters and trace elements). Goodness fit test, decision curve analysis and other related statistical methods were used to perform data analysis. ResultsLower levels of Mg, Ca, Fe in the serum were found in T1D population in females compared with healthy population, while levels of Fe, Zn and Cu of serum in T1D individuals were higher than those of healthy population in males. Levels of serum Mg, Fe and Cu in T1D group were found with significant sex difference for (P < 0.05), and the levels of Fe and Cu in serum of males were higher than those of females, level of serum Mg in males was lower than those of females. Levels of serum Mg and Zn showed fluctuation trend with increased numbers of abnormal clinical parameters (NACP) in males. Serum Zn in females showed consistent elevated trend with NACP; serum Se increased first and then decreased with NACP in males and females. The auxiliary prediction model (Triglyceride, Total protein, serum Mg) was found with the highest predicted efficiency in males (AUC=0.993), while the model in females (Apolipoprotein A, Creatinine, Fe, Se, Zn/Cu ratio) showed the best predicted efficiency (AUC=0.951). The models had passed the verification in validation set, and Chi-square goodness-of-fit test, DCA results both confirmed their satisfactory clinical applicability. ConclusionSex-specific difference were found in serum Mg, Fe and Cu in T1D. The combination of triglyceride, total protein and serum Mg for males, and apolipoprotein A, creatinine, Fe, Se, Zn/Cu ratio for females could effectively predict T1D in patients with negative anti-bodies, which would provide alarm for the population with high-risk of T1D and serve the T1D prediction in patients with negative anti-bodies.

Association between Maternal Selenium Exposure and Congenital Heart Defects in Offspring: A Systematic Review and Meta-Analysis.

The association between congenital heart defects (CHDs) and selenium (Se) is still unclear. We aimed to systematically review and quantitative analyze the potential relationship between maternal Se exposure and CHDs in the offspring. PubMed, Embase, Web of Science and Scopus databases were searched from inception up to August 2021 for relevant studies. Methodological quality of the studies was assessed through Newcastle-Ottawa scale. The Standard mean difference (SMD) and corresponding 95% confidence interval (CI) were calculated to compare maternal Se levels between CHDs groups and control groups using a random-effects model. Four articles covering five studies were included in the systematic review, and three articles covering four studies were included in the meta-analysis. One study measured Se concentrations in maternal hair and found a positive correlation between high concentrations and increased risk of CHDs in offspring. However, one study on cord blood, and one on whole blood illustrated that Se exposure was associated with decreased risk of CHDs. There was no significant association found between serum Se levels and CHDs in two studies. Pooled results showed decreased Se levels in the circulation of mothers with CHDs offspring (SMD = -108.27, 95% CI: -192.72, -23.82), with statistically significant heterogeneity (I2 = 99.8%, P < 0.001) but not in hair, as compared with controls. Low maternal Se status may be associated with increased risk of CHDs in offspring. However, further larger-scale studies with strict and consistent design methods are still required to investigate this issue.

3 Impacts of Vitamin and Mineral Supplementation to Beef Heifers During Gestation on Performance Measures of the Neonatal Calf, Trace Mineral Status, and Organ Weights at 30 Hours After Birth

Abstract Objectives were to evaluate the effects of feeding a vitamin and mineral (VTM) supplement to beef heifers during gestation on calf performance, body measurements, trace mineral status, and organ weights at 30 h after birth. We hypothesized that VTM supplementation during gestation would improve mineral status in the neonatal calf but not impact calf performance parameters and body and organ weights at 30 hours after birth. Fourteen Angus-based heifers [initial body weight (BW)] = 273.7 ± 1.10 kg) were randomly assigned to receive either a basal diet (CON; n = 7) or a basal diet plus a VTM supplement (VTM; n = 7; 113 g•heifer-1•d-1, targeting gain of 0.45 kg/day) from 60 d pre-breeding through gestation. Immediately after parturition, blood samples were collected from dams and calves, then calves were separated from their dams. Calves were fed one feeding of colostrum replacer, followed by milk replacer every 12 h, then euthanized at 30 h. Body weight and measurements were recorded, then organs and viscera were removed, weighed, and sampled. Dam serum and neonatal serum, liver, and blood were analyzed for concentrations of minerals. Data were analyzed using the GLM Procedure of SAS with individual animal as the experimental unit. Dietary treatments did not impact calf weight (0 h or 30 h), calf body measurements, or body weight of the dam at calving (P ≥ 0.32). Further, neonatal organ weights were not influenced (P ≥ 0.21) by maternal VTM treatment. Concentrations of Se and Co in calf serum and Se in calf liver were increased (P ≤ 0.02) by VTM treatment; however, concentrations of Cu, Mn, Mo, and Zn in calf muscle, liver, and serum were not impacted (P ≥ 0.07) by VTM treatment. Concentrations of Co in serum of the dam was the only mineral affected by dietary treatments, being greater (P = 0.001) in VTM than CON dams. In the current experiment providing trace mineral supplementation throughout gestation did not impact calf weight or body measurements at birth. However, the implications of altered mineral status of the neonatal calves at birth, and presumably throughout gestation, may have additional postnatal effects that warrant further investigation.

Changes of serum Se selectin, ACTH, LPS and SIRT1 levels in patients with acute pancreatitis and their correlation with disease severity

This experiment was designed to explore the changes in serum Se selectin, ACTH, LPS and SIRT1 levels in patients with acute pancreatitis and their correlation with the severity of the disease. To do this research, from March 2019 to December 2020, 86 patients with different degrees of acute pancreatitis were selected as the research object. They were divided into mild acute pancreatitis (MAP) group (n = 43), moderately severe acute pancreatitis and severe acute pancreatitis (MSAP + SAP) group (n = 43) and healthy group (n = 43). At the same time, the serum levels of Se selectin, ACTH, LPS and SIRT1 were detected after hospitalization. Results showed that the levels of serum Se selectin, ACTH and SIRT1 in the MAP group and MSAP + SAP group were lower than those in the healthy group; The LPS of the MAP group and MSAP + SAP group were higher than that of the healthy group. The results showed that serum Se selectin, ACTH and SIRT1 decreased with the development of the disease, showing a negative correlation; The change of LPS in patients will increase with the development of the disease, showing a positive correlation. Serum Se selectin, ACTH, SIRT1, and LPS can be used as diagnostic criteria and indicators of acute pancreatitis to achieve early prevention and treatment and improve the prognosis and quality of life of patients.

Relationship between Selected Micronutrient Concentrations, Total Antioxidant Status, Pain Severity, and the Image of 1H MR Spectroscopy in Degenerative Spine Disease: A Case-Control Study.

Osteoarthritis of the spine is a common disease and constitutes a significant problem in highly developed countries. Due to the aging of the population, the number of patients with advanced degenerative changes continues to grow. Trace elements and antioxidant status may play an active role in the pathogenesis of osteoarthritis of the spine. The aim of this study was to assess the relationship between total antioxidant status (TAS), the concentration of selected elements (Zn, Cu, Se, Mn) in the serum, nutritional and environmental factors, the severity of pain, and images of degenerative changes in the spine demonstrated in proton magnetic resonance spectroscopy (1H MRS). The study included 90 patients with degenerative spine disease and 40 healthy people. Serum Zn, Cu, Se, and Mn concentrations were determined by atomic absorption spectrometry. The Cu/Zn molar ratio was calculated. TAS was determined spectrophotometrically using a ready-made Randox kit. The severity of spine pain was assessed using the visual analogue scale VAS. In the 1H MRS study, the fat/water ratio was determined in L1 and L5 vertebral bodies and in the L4/5 intervertebral disc. In patients with osteoarthritis of the spine, there was a reduced concentration of Zn and Cu in the serum (0.77 ± 0.22 mg/L, 1.1 ± 0.35 mg/L) compared to the control group (0.83 ± 0.13 mg/L, 1.25 ± 0.41 mg/L, respectively). In the study group, we also observed a significantly lower Cu concentration in smokers (1.07 ± 0.35 mg/L) compared to non-smokers (1.17 ± 0.34 mg/L). A relationship between the female sex and a decreased concentration of TAS in the serum, both in people with degenerative spine disease and in healthy people, has been demonstrated. In patients with serum TAS levels below the reference values, the fat/water ratio was higher in the L5 spine shaft, while in those with elevated Mn levels, the value was higher in the L4/L5 intervertebral disc, which indicates a greater degree of degeneration in both cases. Patients with normal serum Cu concentration experienced lower pain intensity assessed in the VAS scale. The study showed some abnormalities in serum trace element levels and TAS in patients with osteoarthritis of the spine.

Study the role of oxidative stress in helicobacter pylori induced gastro-intestinal diseases

Background: Helicobacter pylori (H. pylori) is a multi-flagellated, spiral-shaped, Gram-negative, microaerophilic, slow-growing bacteria and extremely mobile which colonizes the stomach mucosa of a human where it causes a long-lasting infection which consistent with either acute or chronic stomach inflammation. Current study was aimed to evaluate total antioxidant capacity (TAC) and selenium (Se) levels in the serum of H. pylori infected individuals and to elucidate the relationship between the two biomarkers. Methods: Two study groups were enrolled in a current case control study; first group was included fifty five subjects with H. pylori infection which considered as H. pylori-associated patients (HPP), and the second group included thirty five apparently healthy subjects as healthy control (HC). Subjects were selected during the period between November 2021 and June 2022 after stringent application of the eligibility criteria. The HPP group subjects were underwent endoscopy and stool antigen test (SAT) and then both study groups subjects were evaluated for their serum anti-H. pylori antibodies (Abs), TAC and Se. Results: The findings revealed a significantly lower serum TAC and Se in HPP group in comparison with HC group. Serum immunoglobulin gamma (IgG) anti-H.

Serum Selenium Levels in Patients With Graves Disease With or Without Thyroid Ophthalmopathy

BackgroundGraves disease is one of the most common autoimmune thyroid diseases. Thyroid has the highest concentration of selenium (Se) in the body. Se plays a crucial role in the functioning of some thyroid enzymes; however, there are controversial results regarding the administration of serum Se levels in patients with Graves disease. MethodsIn this study, patients with Graves disease with orbitopathy (GO group) or without orbitopathy (GD group) were recruited. Healthy individuals without a history of any disease were enrolled as the control group. Serum Se and thyroid hormone levels, including T3, T4, and thyroid-stimulating hormone (TSH), were measured using atomic absorption and radioimmunoassay techniques, respectively. ResultsIn this cross-sectional study, 60 and 56 patients and 58 healthy subjects were included in the GO, GD, and control groups. Serum Se levels in the GO, GD, and control groups were 94.53 ± 25.36 μg/dL, 96.82 ± 30.3 μg/dL, and 102.55 ± 16.53 μg/dL, respectively (P = .193). There was a reverse association between the serum Se level and thyroid hormones, including T3, T4, and TSH, in the GO group. However, serum Se levels exhibited a significant reverse association with T4 and TSH hormones but not with T3 in the GD group. ConclusionOur results showed no significant differences in the serum Se levels in the GO and GD groups compared with that in the control group. In addition, we did not detect any significant difference in the serum Se levels between the GO and GD groups.

Comparison between Macro and Trace Element Concentrations in Human Semen and Blood Serum in Highly Polluted Areas in Italy.

Macro and trace elements are important regulators of biological processes, including those ones linked to reproduction. Among them, Ca, Cu, Fe, K, Mg, Mn, Na, Se, and Zn ensure normal spermatic functions. Hence, the aim of this study was to evaluate the concentrations of 26 macro and trace elements (Al, As, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Sb, Se, Sn, Sr, U, V, and Zn) in blood serum and also in semen of healthy young men, homogeneous for age, anthropometric characteristics, and lifestyle, living in three highly polluted areas in Italy. Furthermore, a comparison among three geographical areas was performed to highlight any difference in the investigated parameters and, overall, to speculate any correlations between chemical elements and semen quality. Statistically significant differences (p < 0.05) among the three areas were found for each investigated element, in both semen and serum samples, where inter-area differences were more evident in semen than in blood serum, suggesting human semen as an early environmental marker. Considering the homogeneity of three cohorts, these differences could be due more to environmental conditions in the recruiting areas, suggesting that variations in those involved in reproductive-associated pathways can have an impact on male fertility. Nevertheless, more research is needed to evaluate threshold values for sperm dysfunction and male infertility. Actually, the role of different dietary intake and environmental exposure underlying the observed differences in the recruiting areas is under further investigation for the same cohort.