Serum GPT Activities Research Articles

Interference of DPPD with hepatic drug metabolizing enzyme system

Summary The direct effects by in vivo administered DPPD on hepatic microsomal enzyme activities have been investigated with the aim to elucidate the mechanism of protection by this compound against toxic liver injuries. The efficiency of hepatic drug metabolizing system has been evaluated both in vitro and in vivo . Furthermore, liver necrosis (serum GPT activity) was estimated in rats poisoned with carbon tetrachloride after DPPD treatment. The administration of DPPD lowers down the activity of liver aminopyrine demethylase and enlarges the hexobarbital sleeping time. CCl 4 alone inhibits the microsomal enzymes, while a preliminary treatment with DPPD fails in preventing but renders more remarkable the block induced by the halogenoalkane. However, the antioxidant completely protects against liver necrosis in CCl 4 -poisoned rats. These findings support the hypothesis that the biological activities of DPPD can be mediated also by inhibition of drug metabolizing enzymes bound to the endoplasmic reticulum.

A New Method for the Assay of Serum GPT Activity using a Selective Color Reaction of Pyruvic Acid with p-Dimethylaminobenzaldehyde

A New Method for the Assay of Serum GPT Activity using a Selective Color Reaction of Pyruvic Acid with p-Dimethylaminobenzaldehyde

Correlation between the Values of Serum GOT and GPT Activities Obtained by the Diazotized Sulfanilic Acid Method and the Improved Reitman-Frankel Method

Correlation between the Values of Serum GOT and GPT Activities Obtained by the Diazotized Sulfanilic Acid Method and the Improved Reitman-Frankel Method

Influence of Cigarette Smoking on Liver Function and Lipids Levels Tests: A comparative Study Directed among Smokers and Non-smokers in Babylon Governorate

Cigarette smoking currently is considered as one of the greatest problems in public health worldwide and is risk factor for peripheral vascular disorders and heart disease. The monitoring of liver function and total cholesterol are very important to give an estimation of the future cardiovascular diseases among smokers. The objective of this study is to evaluate the effect of cigarette smoking on the serum levels of liver enzymes: aspartate aminotransferase (GOT), alanine transaminase (GPT) and cholesterol activities among Iraqi smokers in Babylon City, compared to apparently healthy individuals (non-smokers) as a control group (all were males). A case- control study was carried out on forty Iraqi male smokers who smoke at least 10 cigarettes per day for at least15 years. The group includes smokers with age range between 15-55 years. Non-smokers, (control, n= 20) group were collected with the same range of age for statistical comparison. The whole blood samples were drawn by venipuncture from each individual; levels of cholesterol and liver functions test were estimated in the blood serum of smokers and non-smokers by diagnostic kit (Randox corporation-UK) using automatic analyzer.The findings of this study showed that there was a significant higher level of total cholesterol, ALT, and AST in the smokers group compared to non-smokers (P<0.05). As well as, the results showed a significant positive correlation between the smokers’ age and serum GOT, GPT and cholesterol activity (Increasing) especially in age between 45 to 55 years old, as compared to control. In addition, total cholesterol and liver function enzymes were significantly positive correlated with the duration of smoking (P<0.05).Cigarette smoking leads to oxidative stress by free radical generation by the mechanism of lipid peroxidation that is affected by the heaviness of smoking. Smoking exerts negative influence on liver functions test that should be carefully interpreted and preventive strategies needed to avoid the future cardiovascular diseases.

うつ血性心不全時における肝障害の臨床的実験的研究

Eighty-nine rabbits weighing about 3Kg were employed. Introduction of talcum powder and slices of sponge into the pericardium of the animals resulted in constrictive pericarditis, which was complicated with congestive heart failure following the intake of salt and the loading with physical exertion. These animals were observed for 112 days postoperatively, as to serum bilirubin, protein fraction, colloidal reactions, ALP, ACP, ChE, GOT, GPT, G6P, ALD, PHI and LDH activities. In the meantime studies were made of enzymatic activities in the serum, liver tissue and myocardium, in reference to histological findings of liver tissue.1) About a week postoperatively there were observed an increase in total bilirubin and in direct bilirubin and a decrease in total protein, about seven weeks postoperatively abnormalities of cobalt reaction were observed.2) Regarding serum enzymatic activities, about a week postoperatively a marked increse was observed in glycolytic enzymes, transaminases especially G6P and LDH. Four-five weeks postoperatively the increase temporarily returned to normal, but again showed a slight increase about 10 weeks postoperatively.3) Enzymatic activities in the liver and the myocardium decreased about a week postoperatively, reaching nearly a normal range about four weeks postoperatively followed by a decrease again about ten weeks postoperatively. Thus they showed a mirror image relationship with serum enzymatic activities.4) Liver tissue findings showed acute liver congestion about a week postoperatively, revealing an increasing cellular vacuolization about four weeks postoperatively, followed by a proliferation of the connective tissue of the central lobule in some cases 16 weeks postoperatively.

Serum transaminase (GOT, GPT) and lactic dehydrogenase activity during treatment with methyl testosterone

Oral administration of 30 mg of methyl testosterone in 40 patients caused an increase in the serum GOT and GPT activity within several days from normal to pathological levels in most cases. Less frequently the lactic dehydrogenase activity increased. After this initial rise, the elevated enzyme activity returned rapidly to normal even when treatment was continued. Similar, though less marked, changes in the enzyme activity were observed in 12 patients who received 10 mg of methyl testosterone daily. Ten patients were treated daily with 25 mg of testosterone propionate intramuscularly and another 10 patients received daily 25 mg of testosterone propionate orally over an equal period of time. Testosterone propionate caused no changes in the serum enzyme activity. The pathogenesis of the observed increase of serum GOT and GPT and of lactic dehydrogenase after methyl testosterone treatment is discussed.