Serum Biochemical Profile Research Articles

Molecular Detection of Anaplasma in Cattle of Morang, Rupandehi and Surkhet districts of Nepal

Anaplasma, a vector transmitted rickettial parasite, infects blood cells of bovine causing asymptomatic to clinical bovine anaplasmosis. This study was conducted to estimate the prevalence of Anaplasma sp. in blood samples of dairy cattle in Morang, Rupandehi, and Surkhet districts of Nepal and to determine the risk factors and biochemical alterations of the respective animal. In total, 120 blood samples (40 from each district) were randomly collected from dairy cattle. The blood smears were prepared and fixed with 10% methanol on the field, and fresh blood was collected in EDTA tubes for molecular analysis and in clot activating tubes to yield serum for biochemical tests. 9 (7.5%) blood samples had produced the 577 base pairs of DNA fragments specific for the 16S rRNA gene of Anaplasma sp. by PCR amplification. 5 (4.16%) blood samples in the smear were found positive by microscopic examination. Serum biochemical profile was not different between Anaplasma sp. positive cattle and negative cattle (p > 0.05). The prevalence rate between Jersey and Holstein Friesian crossbred was also not different (p>0.05). There was a higher prevalence among cattle aged more than 2 years compared to cattle less than 2 years (p<0.05) indicating older cattle were more susceptible to the Anaplasma infection. In conclusion, serum biochemical alterations and risk factors should be considered in order to achieve prognosis and initiation of an appropriate therapeutic regimen for a favorable outcome of the tick-borne haemoparasitic diseases along with molecular detection for improved detection.

Potential Influence of Shading in Freshwater Ponds on the Water Quality Parameters and the Hematological and Biochemical Profiles of Nile tilapia (Oreochromis niloticus Linnaeus, 1758)

Nile tilapia is a high-demand commodity in most developing countries including the Philippines, but its production is hampered by fluctuating water temperature (WT) and dissolved oxygen (DO) concentrations, which are mostly influenced by temperature warming. Net shading has been demonstrated to reduce pond temperature and increase the rate of spawning in cultured tilapia. The current study aimed to investigate the potential effects of shading on the water quality parameters, blood indices, and serum biochemical profiles of cultured Nile tilapia. Fish were collected from unshaded (control) and shaded (experimental) ponds in three breeding cycles. WT and DO concentrations were measured, while hematological and biochemical analyses were performed. The results showed that the average WT was considerably decreased in the shaded pond with no significant difference in the DO concentrations. Red blood cells, hemoglobin, and lymphocyte counts were significantly lower in fish raised in shaded ponds, although mean corpuscular volume and neutrophil counts were significantly higher. Likewise, Chole, TP, and Alb values were significantly affected by the interaction of cycle and shading setup. Cortisol, Glb, K+, Cl−, Mg2+, and TCa values showed significant differences and were only affected by the cycle. Similarly, AST and ALT values showed significant differences and were affected by both cycle and shading setup. Water quality parameters (WT and DO), hematological blood indices, and serum biochemical variables were correlated positively with each other. In brief, prior data imply that net shading can reduce WT in aquaculture ponds, reduce stress in Nile tilapia, and lessen the consequences of temperature warming on species production.

Effect of feeding different levels of forages and concentrate ration on production performance, serum biochemical and hematological profile in Ghaljo sheep (Ovis aries)

Effect of feeding different levels of forages and concentrate ration on production performance, serum biochemical and hematological profile in Ghaljo sheep (Ovis aries)

Case report on successful treatment for brain abscess in a Japanese monkey (Macaca fuscata)

BackgroundA brain abscess in human beings is a focal infection of the central nervous system frequently characterized by areas of localized cerebritis and central necrosis surrounded by a well vascularized capsule. A brain abscess, although sporadically reported, is relatively rare disease in domestic animals (horses, cattle, goats and alpacas), companion animals (dogs and cats) and laboratory nonhuman primates. Brain abscesses are life threatening disease that needs early and aggressive veterinary therapy.Case presentationThe purpose of this study on a brain abscess in a Japanese monkey was to report the investigational and therapeutic processes including clinical observations, hematological and serum biochemical profiles, and magnetic resonance imaging (MRI) features, probiotic and antibiotic therapy. In clinical observation, the monkey presented with slowly progressive gentle and depressed behavioral change. Hematological findings showed that slightly declined platelet counts gradually increased in the course of the treatment. Serum biochemical profiles revealed initial markedly elevated. A series of chemotherapy provide prominent relief from the influence of the brain abscess. MRI images illustrated that a brain abscess was located in the right frontal lobe and the mass was delineated by a thick rim, indicating the capsule formation stage. The lesion chronologically decreased in size over the course of treatment. Until 11 weeks after treatment of the brain abscess, the size of brain abscess continued to reduce, leaving an organized lesion trace. To the best of my knowledge, this is the first report on successful treatment for a brain abscess in a Japanese monkey (Macaca fuscata).ConclusionsMedical management of simian brain abscesses is possible based on the controlled and resolving nature of the lesions as determined by MRI and completion of a of chemical antibiotic treatment presented in this study.

Dietary Ulva lactuca and CAZyme supplementation improve serum biochemical profile and hepatic composition of weaned piglets

Ulva lactuca is a seaweed with antinutritional cell wall for monogastrics. Carbohydrate-Active enZymes (CAZymes) supplementation can potentially cause its disruption. This study evaluates four diets: Ctrl—control diet; UL—control + 7% U. lactuca (wild caught, powdered form); ULR—UL + 0.005% Rovabio® Excel AP; ULU—UL + 0.01% ulvan lyase on piglets’ haematologic and serologic profiles, hepatic lipids and minerals. White blood cells and lymphocytes reached the highest values in piglets fed UL compared to control, and to control and ULR; respectively (P < 0.05). IgG levels were boosted by seaweed incorporation compared to control (P = 0.015). The glycaemic homeostasis was assured by the seaweed inclusion. Dietary seaweed decreased serum lipids (P < 0.001), with the exception of ULU, due to HDL-cholesterol increase (P < 0.001). Cortisol was decreased in ULR and ULU (P < 0.001). No systemic inflammation was observed (P > 0.05). While hepatic n-3 PUFA increased in piglets fed with seaweed diets due to increment of beneficial 22:5n-3 and 22:6n-3 fatty acids (P < 0.05), the opposite occurred for n-6 PUFA, PUFA/SFA and n-6/n-3 ratios (P < 0.05). Hepatic pigments were unchanged (P > 0.05). ULR reduced α-tocopherol levels (P = 0.036) and increased serum potassium levels (P < 0.001) compared to control. Seaweed contributed to overcome piglets’ weaning stress, with some benefits of including CAZyme supplementation.

Measure the Serum Biochemical Profiles (Glucose, Protein, Cholesterol) of Female Black Bengal Goats during Growing and Pregnancy Period

Measure the Serum Biochemical Profiles (Glucose, Protein, Cholesterol) of Female Black Bengal Goats during Growing and Pregnancy Period

A Study of Heavy Metal Status and its Relationship with Hematologic and Biochemical Indices in River Buffaloes in Southwest Iran.

Heavy metals are among the most important environmental pollutants which accumulate in various organs and are associated with several toxic effects. This study was performed to determine the status of heavy metals in river buffaloes in Khuzestan province, Iran, and its relationship with hematologic and serum biochemical parameters. A total of 103 apparently healthy buffaloes were sampled from the region. The concentration of heavy metals, including lead (Pb), mercury (Hg), and cadmium (Cd), was determined in serum samples by atomic spectroscopy. In addition, complete blood counts and serum biochemical profiles were assessed. The serum concentration of Cd, Pb, and Hg in the sampled buffaloes, as mean±standard error, were 0.55±0.01, 6.51±0.10, and 6.28±0.09 µg/l, respectively, which are within the permissible serum levels in the livestock. Serum Cd and Hg levels showed no significant relationship with hematologic or biochemical analytes. However, there were significant negative correlations between Pb levels and phosphorus, magnesium, sodium, as well as potassium concentrations, while serum iron was positively correlated with lead (P<0.05). In addition, there was a significant positive correlation between Hg level and serum aspartate aminotransferase activity (P<0.05). Despite the fact that river buffaloes in Khuzestan spend a long time daily in the Karun River with high industrial pollution, no serum evidence of heavy metal toxicity was found in these animals. It can be suggested that river buffaloes in Khuzestan seem to be resistant to the environmental pollution caused by heavy metals. However, further studies are required to confirm this issue and identify its possible explanations.

Protective effect of aqueous extract of Pleurotus eous against Dalton's lymphoma ascites tumor in mice

The antitumor efficacy of Pleurotus eous aqueous extract (PEAE) was investigated both in vitro and in vivo. The MTT test was used to determine in vitro cytotoxicity against the Dalton's Lymphoma Ascites (DLA) cell line. PEAE's in vivo antitumor effect was compared to that of the conventional drug cyclophosphamide in a DLA-induced solid tumor model. After 24 h following DLA inoculation, experimental animals were given PEAE (500 and 1000 mg/kg bw) and cyclophosphamide (CTX, 25 mg/kg bw) orally for 30 days. PEAE was also subjected to preliminary phytochemical screening as part of the research. PEAE has a high total phenolic (9.63 GAE mg/g) and total flavonoid (3.81 CAE mg/g) content, according to phytochemical analyses. The phytochemical components of PEAE were identified by HPTLC analysis, which revealed three phenolic and one flavonoid compound peaks. PEAE exhibited antioxidant capacity, as per ABTS and FRAP assays. At 1000 μg/mL, anti-proliferative activity against the DLA cell line was moderately inhibited. PEAE reduced tumor burden in a dose-dependent way. At 500 and 1000 mg/mL PEAE treatment, there was a significant decrease in tumor volume (62.47 and 72.13%), tumor weight (53.32 and 73.72%), and improved longevity (30.89 and 58.14%). PEAE restored normal haematological and serum biochemical profiles in a dose-dependent manner, with a concurrent decrease in lipid peroxides and an increase in enzymic and non-enzymic antioxidants. Major chemical components of PEAE may be responsible for the anticancer effect, which was attributed to its enhancing endogenous mechanism, according to this study. The usefulness of PEAE as a possible naturally sourced therapeutic target for tumor prevention is emphasised.

Studies on serum biochemical profile in Amrit Mahal breed of cattle

Studies on serum biochemical profile in Amrit Mahal breed of cattle

Haematological and Serum Biochemical Indices of West African Dwarf Rams Fed Ensiled Elephant Grass and Gmelina Arborea Leaves

In a study to evaluate the haematological parameters and serum indices of rams fed with ensiled Elephant grass and Gmelina arborea leaves, 16 WAD rams were randomly assigned to four dietary treatments for 4 weeks. Each treatment was replicated thrice in a Completely Randomized Design (CRD). The data obtained were subjected to analysis of variance (ANOVA) using procedure of SAS (2002); where analysis indicated significant difference, the significant means were compared using the Least Significant Difference method. Water and feed were given ad-libitum. Major parameters measured included: Red Blood Cell (RBC), Packed Cell Volume (PCV), White blood Cell (WBC), Hemoglobin, lymphocytes, neutrophils, monocytes, Total Protein (TP), Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), albumin, glucose, urea, creatinine, cholesterol, High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL). The four dietary treatments were 80% Elephant grass+ 20% cassava peels+0% Gamhar (T1), 60% Elephant grass+ 20% cassava peels+20% Gamhar (T2), 20% Elephant grass+ 20% cassava peels+60% Gamhar (T3), and 0% Elephant grass+ 20% cassava peels+80% Gamhar (T4). Except for red blood cell (RBC), white blood cell (WBC), monocytes, creatinine, urea and LDL, all the haematological and biochemical parameters measured were significantly (p˂0.05) different across the dietary treatments. It was observed that the ensiled diets offered to the rams did not have deleterious effect on the haematological and serum biochemical indices as the values registered across the dietary treatments falls within the normal ranges. It was concluded that all four test diets were suitable for dry season ram feeding and that Gmelina arborea could be included in ram diets up to 80% without any harmful effect on their haematological and serum biochemical profile.

Ganoderma lingzhi culture enhance growth performance via improvement of antioxidant activity and gut probiotic proliferation in Sanhuang broilers.

The experiment was conducted to evaluate the effects of Ganoderma lingzhi culture (GLC) as a fermented feed on growth performance, serum biochemical profile, meat quality, and intestinal morphology and microbiota in Sanhuang broilers. In addition, the association between gut bacteria and metabolites was investigated via untargeted metabolomic analysis. A total of 192 Sanhuang broilers (112 days old) with an initial body weight of 1.62 ± 0.19 kg were randomly allocated to four treatments, six replicate pens per treatment with 8 broilers per pen. The four treatments contain a control diet (corn-soybean meal basal diet, CON), a positive control diet (basal diet + 75 mg/kg chlortetracycline, PCON), and the experimental diets supplemented with 1.5 and 3% of GLC, respectively. The trial includes phase 1 (day 1-28) and phase 2 (day 29-56). The results showed that broilers in PCON and GLC-added treatments showed a lower FCR (P < 0.05) in phase 2 and overall period and a higher ADG (P < 0.05) in phase 2. On day 56, the concentrations of serum SOD (P < 0.05), and HDL (P < 0.05) and cecal SCFA contents (P < 0.05) were increased in broilers fed GLC diets. Broilers fed GLC also showed a higher microbiota diversity and an elevated abundance of SCFA-related bacteria in the caecum. The association between intestinal bacteria and metabolites was investigated via correlation analysis. The differential metabolites in the caecum, such as L-beta-aspartyl-L-aspartic acid and nicotinamide riboside, were identified. In summary, dietary GCL supplementation could increase growth performance to some extent. Moreover, GLC might benefit broilers' health by improving serum HDL content, antioxidant status, SCFAs contents, bacterial diversity, and probiotic proliferation in the caecum.

Role of Clove and Tulsi on broiler health and meat production

The role of Clove (Cv) and Tulsi (Ts) supplementation on broiler growth performance and gut health as an alternative to antibiotic growth promoters has already been established. Therefore, the objectives of this study were to investigate the role of Cv and Ts on the serum biochemical profile and meat quality traits in broilers. A total of sixty (60) one-day-old commercial broiler chicks were randomly allotted into four homogenous groups (15 birds per group). They were then fed Cv powder and Ts extract from day (d) 8 to d 28 with drinking water i.e. zero level of Cv or Ts (T0), 0.5% Cv + 2% Ts (T1), 1.0% Cv + 3% Ts (T2), 1.5% Cv + 4% Ts (T3). Blood and meat samples were collected on d 14, 21, and 28 to analyze the serum-biochemical profile and meat quality. Supplementation of Cv and Ts improved serum-biochemical profile by reducing total cholesterol and low-density lipoprotein levels in broilers. However, they did not affect the serum protein levels while the 0.5–1% Cv and 2–3% Ts supplemented groups had higher glucose levels on d 21. Production of breast and thigh meat increased with increased size, and density of myofibers while fed 0.5–1% Cv and 2–3% Ts. On the contrary, 1.5% Cv powder and 4% Ts extract supplementation improved thigh meat color, breast and thigh meat pH as well as the water-holding capacity. The current study findings suggest that Cv and Ts can be used in combination to improve broiler health, production, and meat quality.

Evaluation of herbal leaf meal as an alternative to in-feed antibiotics on haematological parameter and serum biochemical indices of growing sexed pigs

This study was conducted at the Federal University of Agriculture, Abeokuta in September 2019 to evaluate the influence of sex and sole or combined herbal leaf meal as alternative in-feed antibiotics on serum biochemical indices. A total of thirty large white sexed pigs of eight weeks were allotted into five (5) dietary treatments for 12 weeks. Dietary treatments had no significant effect on haematological parameters and serum biochemical indices. Sex did not influence the haematological and serum biochemical profile of pigs except for monocyte, albumin, aspartate aminotransferase, and cholesterol respectively. Dietary supplementation did not influence the haematological serum biochemical profile of pigs. This indicates that the haematological indices were not influenced by sex and diet except the significant values which were within the normal range for sexed pigs which reveals that sex and diet does not compromise the immune system of the sexed pigs.

Effects of Feeding Oak Acorn Flour on Hematology and Serum Biochemical Profile, and Carcass Characteristics of Japanese Quail

Effects of Feeding Oak Acorn Flour on Hematology and Serum Biochemical Profile, and Carcass Characteristics of Japanese Quail

Serum biochemical changes in cats with naturally acquired feline cytauxzoonosis

The geographical distribution of feline cytauxzoonosis is expanding in the US. Clinical signs of feline cytauxzoonosis, including lethargy, anorexia, and icterus, are similar to hepatic lipidosis and cholangiohepatitis. Hematologic and serum biochemical abnormality patterns may assist practitioners in prioritizing feline cytauxzoonosis as a differential diagnosis over hepatic lipidosis and cholangiohepatitis. Hematology and serum biochemical profiles of cats with naturally acquired feline cytauxzoonosis, hepatic lipidosis, or cholangiohepatitis. Retrospective search and analysis of the Kansas State Veterinary Diagnostic Laboratory or Kansas State University Veterinary Health Center records between January 2007 and June 2018 for cats with cytauxzoonosis, hepatic lipidosis, or cholangiohepatitis. Patients with acute feline cytauxzoonosis presented with frequent nonregenerative anemia (20/28 [71%]), leukopenia (23/28 [82%]), thrombocytopenia (23/23 [100%]), hyperbilirubinemia (27/28 [97%]), hypoalbuminemia (26/28 [93%]), reduced (18/28 [64%]) or low normal (10/28 [36%]) serum ALP activity, and hyponatremia (23/28 [82%]). Reduced ALP activity was unique to cats with feline cytauxzoonosis relative to hepatic lipidosis and cholangiohepatitis. No correlation between the severity of anemia and the magnitude of hyperbilirubinemia was identified in feline cytauxzoonosis patients. The combination of nonregenerative anemia, leukopenia, thrombocytopenia, hyperbilirubinemia, and reduced serum ALP activity in icteric cats may increase the clinical suspicion, but is not pathognomonic, for acute feline cytauxzoonosis. Hematologic and serum biochemical abnormalities of naturally acquired feline cytauxzoonosis are like those reported with feline bacterial sepsis. Blood smear evaluation for intraerythrocytic Cytauxzoon felis piroplasms, tissue aspirates for schizont-laden macrophages, and/or molecular testing are required to diagnose feline cytauxzoonosis.

Serum biochemical reference interval determination inwild Siberian weasel (Mustela sibirica).

Determining reference intervals (RI) isavaluable asset for assessing the health ofwildlife species. This isthe first study toestablish serum biochemical RIs inSiberian weasels. Forty-two healthy free-ranging Siberian weasels were captured live and brought toSeoul Wildlife Center between June 2021 and August 2022. Blood samples from 42healthy Siberian weasels ofboth sexes were used tocalculate RIs. Anautomated analyser was used toperform serum biochemistry profiles. The American Society for Veterinary Clinical Pathology recommendations were used tocalculate anonparametric RI with 90% confidence intervals. The RIs ofalbumin, total protein, globulin, calcium, glucose, blood urea nitrogen, phosphorus, amylase, cholesterol, alanine aminotransferase, total bilirubin, alkaline phosphatase, creatinine, and creatine kinase were determined. The RIs established inthis study will serve asagood starting point for analysing serum biochemical data inSiberian weasels.

Lactation stages modulate the hematological, serum biochemical, and endocrinological profiles and oxidative stress markers in crossbred cows under tropical humid island ecosystem of Andaman and Nicobar Islands

The present study was designed to assess the interrelationship between hematological, serum biochemical, and endocrinological profiles and oxidative stress markers and lactational stages in crossbred (CB) dairy cows of Andaman and Nicobar Islands (ANI). Healthy (n = 6) CB cows (50-62.50% exotic inheritance; Holstein Friesian × Andaman local) of 4th parity with age of 7-9years and body weight of 350-400kg were selected from Cattle Breeding Farm, ICAR-Central Island Agricultural Research Institute (ICAR-CIARI), Port Blair, ANI. These experimental cows were synchronized with Ovsynch protocol, and parturition was planned to happen in the month of May-June. Lactation was allowed for 305days. Hematological profiles, serum biochemical profiles, oxidative stress markers and endocrinological profiles were measured at a 15-day interval from day 07 to 305 of lactation (after 6days of colostrum). The lactation period was divided into first (day 07 to 90), second (day 91 to 180), and third (day 181 to 305) stage of lactation. Average daily milk yield (L) did not vary among the stages of lactations; however, first (8.56 ± 1.26) and second (9.79 ± 0.87) stages had higher milk yield compared to third (7.93 ± 0.79) stage of lactation. Hematological profiles did not vary among the stages of lactation; however, these values were within the range of bovine species at lactation. Serum glucose, triglyceride, total cholesterol, total protein, globulin, and blood urea nitrogen (BUN) increased (P < 0.05) and albumin and creatinine decreased (P < 0.05) gradually as lactation stages advanced. Activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and concentration of calcium, phosphorous, and magnesium were nearly similar among the stages of lactation. Similarly, triiodothyronine (T3), and thyroxine (T4) increased and prolactin and cortisol decreased (P < 0.05) gradually as stages of lactation advanced. Total antioxidant capacity (TAC) increased and malondialdehyde (MDA) decreased (P < 0.05) as lactation stages advanced. The results of the present study indicated that the lactating CB cows suffered nutritional stress (deficiency of protein, carbohydrate, lipids, and minerals), physiological stress (higher cortisol), oxidative stress (higher MDA and deficiency of total antioxidant capacity), and hormonal imbalance (higher prolactin and cortisol and deficiency of thyroid hormones) during the early stages of lactation. Thus, the first and second stages are more stressful events compared to the third stage of lactation in the CB cows in ANI. Therefore, regular monitoring of blood components and accordingly suitable feeding strategies with balanced nutrients and minerals, supplementation of suitable antioxidants, and appropriate management practices need to be implemented to mitigate these stresses and to prevent metabolic disorders with maximum milk production during different stages of lactation in CB cows under humid tropical island ecosystem of Andaman and Nicobar Islands.

Thymic cavernous haemangioma in a dog

A 12-year-old male neutered Australian Shepherd Dog was presented to Charlotte Animal Referral & Emergency with a history of a thoracic mass. On physical examination, physiological parameters were within the normal ranges, and a complete haemogram and serum biochemistry profile were unremarkable except for mild thrombocytopenia. A computed tomography scan revealed a 21 × 15 × 12.7 cm thoracic mass encompassing the cranial mediastinum and extending to the right caudal thorax. The mass was surgically removed and histopathological evaluation revealed that it comprised remnants of the thymus and a neoplasm composed of large blood-filled vascular spaces lined by a single layer of endothelial cells with mild anisocytosis and anisokaryosis. The neoplastic cells had diffuse strong immunolabeling for endothelial cell marker CD31. Multifocally, there were large cystic degenerated areas of thymic tissue lined by plump cytokeratin AE1/AE3-positive epithelial cells. Based on these findings, a diagnosis of thymic cavernous haemangioma (CH) was made. Thymic CH is rare in animals, with the only reported case in a cross bred cow. To our knowledge, this is the first case of a thymic CH in a dog.

Proximate, Electrolyte and Biochemical Profile of Rabbits Fed with Tridax procumbens Residue Meal in Replacement of Vitamins and Minerals in Lapai Niger state Nigeria

Proximate, electrolyte and biochemical profile of rabbits fed with Tridax residue meal replacing Vitamin and Mineral were evaluated in Lapai, Niger State, Nigeria using standard techniques. The dietary treatments evaluated were 20%, 40%, 60%, 80% and 0% inclusion level of Tridax. The results obtained did not follow any pattern except Urea, Globulin and Creatinine which had significant (P&lt;0.05) decrease in the serum biochemical profile. The highest value for crude fat was seen in feed3 with value of 11.33±0.30 and the lowest was recorded in the control feed with value of 10.38±0.21. There was no significant difference in crude protein between feeds 1 and 2 with values of 58.13±0.15 and 56.13±0. 15 but feeds feeds3, 4 and the control feed showed significant difference at (P&lt;0.05) The result of Chloride shows no significant differences (P&lt;0.05) in feeds1, 2 and 3, with values of 7.26±0.96, 7.25 ±3.74 and 7.55±4.81 also feeds4 and the control feed shows no significant difference with values of 6.55±2.67 and 6.46 ± 0.96 but there is significant difference between feeds1, 2, 3 and feeds4 and control feed. The prepared feed supplemented with 20 and 40% Tridax is promising compared to others, hence could be used as a supplement in the feed of rabbit which can be consumed in place of red meat. Also the low cholesterol level in the feeds makes it idle for people with cardiovascular related problem. Thus, the cost of production of feed can be reduced using Tridax plant as a substitute for Vitamin and mineral premix. It is recommended that further studies on the optimum level of inclusion of Tridax plant to be established.

Comparative Assessment of Serum Biochemical Markers from Cattle and Buffaloes Vaccinated with Three Different Oil Adjuvant Vaccines of Hemorrhagic Septicemia from Punjab, Pakistan

Haemorrahgic Septiceamia (H.S) is a deadly, sub-acute livestock disease of cattle and buffalo in Southeast Asia including Pakistan. A whopping 98% case fatality rate renders vaccination a mandatory prophylactic control measure to counter HS. Very little information is available on serum biochemical profile of the HS vaccinated animals. This study was conducted to investigate the serum biochemistry of the vaccinated cattle and buffalo calves and adults alike. Three different experimental oil adjuvant vaccines were produced for Pasturella multocida serotype B:2 (locally prevalent). N=80 healthy cattle and buffalo (calves and adults) were divided into four groups. Each group consisted of 20 animals (10 calves and 10 adults). Group A was injected with vaccine prepared with Montanide ISA-50; Group B with vaccine prepared from Montanide ISA-206 while Group C with a vaccine produced from Liquid paraffin and Lanolin. Group D animals served as non-immunized control. Blood samples were collected from each animal at zero-day, after booster at 90 days, and then every quarter for one year. Twelve biochemical parameters were assessed by ANOVA and Kruskal Wallis Test. Post hoc multiple comparisons were done for glucose levels. It was concluded that vaccinating both cattle and buffalo calves and adults with H.S. vaccine formulated with three different adjuvants did not have a statistically significant effect on serum biochemistry of vaccinated animals compared to controls.