Serum Biochemical Profile Research Articles

Exploring the Role of Serum Osteonectin and Hsp27 in Pediatric MAFLD Diagnosis and Cardiometabolic Health.

Childhood obesity is one of the major challenges of public health policies. The problem of fatty liver in childhood, known as MAFLD (metabolic dysfunction-associated fatty liver disease), is of particular interest as the gold standard diagnosis technique is invasive (liver biopsy). Hence, efforts are made to discover more specific biomarkers for the MAFLD signature. Therefore, the aim of the study was to evaluate Osteonectin and Hsp27 as biomarkers for MAFLD diagnosis and to assess their links with auxological and biochemical profiles of overweight and obese pediatric subjects. A cross-sectional study in which we (re)analyzed data from the MR PONy cohort comprising 71 pediatric subjects. Auxological data, liver ultrasonography and biochemical serum profile were recorded. Lipid-derived indices and body composition indices were calculated. Nevertheless, serum Osteonectin and Hsp27 levels were assessed using an ELISA approach. MAFLD prevalence was 40.8%. Higher Osteonectin levels were noted in MAFLD subjects versus non-MAFLD subjects and in dyslipidemic children regardless of their liver function status. Lipid-derived indices had good diagnostic capacity for MAFLD. We confirm Osteonectin as a MAFLD diagnosis biomarker in children. Also, lipid-derived indices are useful as metabolic-associated organ impairment markers in children even before the onset of obesity.

Cymbopogon schoenanthus (L) extract ameliorates high fat diet-induced obesity and dyslipidemia via reducing expression of lipogenic and thermogenic proteins

Globally, obesity has become one of the major health problems. This study was conducted to evaluate the anti-obesity potential of Cymbopogon schoenanthus methanolic extract (CS) in rats. Fifty male Wistar rats of six to eight weeks old, 100–120 g body weight (BW) were randomly assigned into 5 groups (n = 10): The control group was fed a basal diet. CS-group was supplied with basal diet and orally given CS (200 mg/kg BW) for 12 weeks. HFD-group was fed a high-fat diet (HFD) for 18 weeks. HFD + CS-group was fed on HFD and CS HFD then CS-group was fed HFD for 12 weeks then shifted to basal diet and CS for another 6 weeks. Phytochemical analysis of CS indicated the presence of various terpenes and flavonoid compounds. Among the compounds characterized are quercetin, apigenin, luteolin, orientin, eudesmene, cymbopogonol, caffeic acid, coumaric acid, and linolenic acid. Supplementation of HFD significantly increased the body weight, levels of serum triacylglycerol, total cholesterol, very low-density lipoprotein, low-density lipo-protein (HDL), glucose, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities. In addition, HFD up-regulated the protein expression of uncoupling protein (UCP)-1 in both brown and white adipose tissue; and the expression of hepatic mRNA of sterol regulatory element-binding protein (SREBP)-1c and SREBP-2. However, it decreased the serum level of HDL, and protein expression level of UCP-1 in both brown and white adipose tissue. Treatment of HFD-fed animals with CS extract either concurrently (HFD + CS-group), or after obesity induction (HFD then CS-group) significantly reversed all HFD-induced alterations in body weight; food intake; serum biochemical profile (including hyperglycemia, dyslipidemia); and tissue gene expressions. These results indicate that CS methanolic extract ameliorated HFD-induced obesity, serum biochemical, hepatic, and adipose tissue gene expression alterations. CS extract accomplished these effects mostly through its various identified bioactive compounds which have been proven to have anti-obesity and anti-diabetic activities.

Response of serum biochemical profile, antioxidant enzymes, and gut microbiota to dietary Hong-bailanshen supplementation in horses.

Traditional Chinese medicine (TCM) is widely used in humans and animals, which is very important for health. TCM affects the body 's immunity and changes in intestinal flora. This study was conducted to investigate the effects of dietary Hong-bailanshen (HBLS) supplementation in horses on serum biochemical profile, antioxidant enzymes and gut microbiota. In this study, five horses were selected. On day 0, 14, 28, blood samples and feces were collected on days 0, 14, and 28 to analyse gut microbiota, serum biochemical and redox indexes. The results showed that the addition of HBLS to horse diets significantly decreased the level of alanine aminotransferase, alkaline phosphatase, creatine kinase and malondialdehyde (p < 0.05, p < 0.01) and significantly increased the activity of total antioxidant capacity, superoxide dismutase and catalase (p < 0.05, p < 0.01). Compared with day 14, the levels of alanine aminotransferase, alkaline phosphatase and creatine kinase were significantly decreased; however, the level of catalase was significantly increased in the horses continuously fed with HBLS for 28 days (p < 0.05, p < 0.01). Alpha diversity analysis was performed that chao1 (p < 0.05), observed_specicies, faith'pd and goods_coverage upregulated in the horses fed HBLS. A total of 24 differential genera were detected adding HBLS to diet increased the abundance of Bacillus, Lactobacillaceae, Leuconostocaceae, Christensenellaceae, Peptostreptococcaceae, Faecalibacterium, Erysipelotrichaceae, Pyramidobacter, Sphaerochaeta, WCHB1-25, Bacteria, Oscillospira, and Acetobacteraceae, while reduced Aerococcus, EtOH8, Syntrophomonas, Caulobacter, Bradyrhizobiaceae, W22, Succinivibrionaceae, and Desulfovibrio (p < 0.05, p < 0.01). Adding HBLS to the diet could be a potentially effective strategy to improve horses' health.

Preparation, Characterization, and Sub-Chronic Toxicity of Carvacrol-Self-Nano-Emulsifying Drug Delivery System Towards Healthy Sprague Dawley Rats

Background: Carvacrol (CAR) is the active component in essential oils (EOs) of many fragrant plants, including oregano and thyme; however, its high toxicity restricts its usage in biomedical fields, and the self-nano-emulsifying drug delivery system (SNEDDS) was suggested to overcome this issue. Objective: To prepare and characterize the CAR-loaded SNEDDS and to assess its toxicity profile towards healthy rats using the in vivo sub-chronic study. Methods: SNEDDS was prepared from olive oil, dimethyl sulfoxide, Tween-80, and distilled water, then CAR-SNEDDS was prepared by adding 0.5% CAR to SNEDDS and both composites were gently agitated for 72 h at room temperature. Later on, both composites were physiochemically characterized for size/charge (Zetasizer), shape (TEM), crystallinity (XRAD), composition (FTIR), and quantitated (UV–Vis). Additionally, the sub-chronic toxicity of both composites at different doses was conducted by orally treating healthy Sprague Dawley for 4 weeks. Then, the treated rats were checked for toxicological symptoms, food/water intake, and behavioral abnormality. In addition, the blood samples were tested for hematologic/biochemical changes, while vital organs (liver and kidney) were assessed for histopathological alterations. Results: The average globule size, zeta potential, and polydispersity index of CAR-SNEDDS were 158.93 ± 22.18 nm, −22.56 ± 1.77 mV, 0.553 ± 0.31, respectively. All treated animal tissues, serum biochemical profiles, and total hemograms were normal. At 30-90 mg/kg oral doses, CAR-SNEDDS was not toxic and did not cause mortality. Conclusions: CAR-SNEDDS was successfully synthesized and characterized, and the results from sub-chronic oral toxicity studies showed that the CAR-SNEDDS were non-toxic and safe for biomedical fields.

Comparative evaluation of the serum biochemical and lipid profile changes in spayed male and female rats

Background and aims: This study investigated the effects of gonadal removal in male and female rats on changes in serum biochemical parameters. Methods: Twenty-eight adult male and female rats were divided into four groups of 7 animals for a period of 9 weeks. The first and second groups of intact male and female rats, as well as the third and fourth groups of male and female rats, were gonadectomized, respectively. At the end of the ninth week of the study, the rats were anesthetized with chloroform, and the amount of glucose, some lipid parameters in serum, and the activity of a number of serum enzymes were measured after taking blood from the heart. Results: Alanine aminotransferase, and alkaline phosphatase (ALP) levels were higher in intact male rats than in intact female rats, respectively (P=0.03, P=0.015). The amounts of glucose and cholesterol of low-density lipoprotein (LDL) in intact female rats were higher than in the ovariectomized rats, respectively (P=0.07, P=0.039, P=0.03). The amount of lactate dehydrogenase (LDH) in spayed female rats demonstrated a significant increase compared to other groups (P=0.001). However, there was a significant decrease in calcium levels in gonadectomized rat groups in comparison to intact female rats (P=0.02). Finally, a significant increase was found in phosphorous levels in the intact male rats group compared to other groups (P=0.002). Conclusion: Decreased sex hormones in gonadectomized rats compared with intact rats could lead to increased serum levels of LDH, ALP, and LDL cholesterol that may result in the development of metabolic and atherogenesis syndromes and acute liver failure.

Effects of Choline Supplementation in Diets on Juvenile Pacu (Piaractus mesopotamicus): Productive Performance, Proximate Composition and Serum Lipid Level.

Pacu (Piaractus mesopotamicus) is a Brazilian fish species of high commercial value and has emerged as one of the main species with potential for intensive farming. This study aimed to investigate the impact of choline supplementation on productive performance, visceral fat, hepatosomatic indices, proximate composition, serum biochemical profile, and liver coloration in juvenile pacu (Piaractus mesopotamicus). The 60-day experiment was conducted in the experimental hatchery of the Study Group of Aquaculture Management - GEMAq of the State University of West Paraná, Brazil. The completely randomized design consisted of five treatments with four replicates each. A total of 200 fish, with an average initial weight and length of 4.25 g and 5.97 cm, were distributed among 20 experimental hapas, resulting in a stocking density of 10 fish per experimental unit. Fish fed a choline-deficient diet exhibited the lowest values for final weight, weight gain, visceral fat, and hepatosomatic index. Conversely, in terms of proximate composition, fish fed choline-deficient diets had the highest moisture content but the lowest crude fat content. Triglyceride levels in juvenile pacu were significantly higher in groups fed diets containing choline (400 to 1200 mg kg-1 ). We also observed significant differences in the cyan color of the liver (P < 0.05). Notably, we observed significant differences in the cyan coloration of the liver (P < 0.05). Other parameters did not exhibit significant changes in response to varying choline levels in the diets. Our study demonstrated that choline supplementation in P. mesopotamicus juveniles at an optimal concentration of 400 mg kg-1 significantly impacted growth, metabolism, productive performance, proximate composition, biochemical parameters, and liver color. These findings offer crucial guidance for aquaculture professionals, enabling them to enhance growth and health in P. mesopotamicus farming. Further research on choline's influence on lipid metabolism holds potential for more precise dietary recommendations, ultimately improving the efficiency and sustainability of aquaculture practices for this species.

Effect of Nano Vitamin E and Selenium Supplementation on Serum Biochemical Profile in Japanese Quails

This study investigated the impact of nano vitamin E with Selenium on serum parameters in Japanese quails. The experiment involved three groups: a control group (C1) fed a basal diet, a T1 group receiving vitamin E (150mg/kg) and Selenium (0.3mg/kg), and a T2 group receiving nano vitamin E (15mg/kg) and Selenium (0.03mg/kg). Results revealed significant differences (p&lt;0.05) in serum analyses among the groups. The T2 group showed the highest total protein and globulin levels, while the T1 group exhibited the highest total albumin levels. Total cholesterol levels were significantly lower in the T2 group, with the highest HDL cholesterol. LDL cholesterol levels were lower in the T2 group. SGOT and SGPT levels were significantly lower in the T2 group. These findings suggest the potential benefits of nano vitamin E with Selenium in modulating serum biochemistry in Japanese quails.

Serum biochemical profiles between repeat breeder and normal cyclic jersey crossbred cows

Serum biochemical profiles between repeat breeder and normal cyclic jersey crossbred cows

Haemogram and blood cell morphological changes in haemoparasitic infection of poultry birds

Aim and Background: The poultry industry faces myriads challenges, necessitating effective management and control strategies. Thus, this study was designed to identify haemoparasite and determine the impact of haemoparasitic infection on blood cells and serum biochemical profiles in some poultry birds in Ibadan, Nigeria. Methods: The study examined 390 birds of different types and of various types for the prevalence and type of haemoparasites, haematological parameters, and associated risk factors. Thin smears were prepared from each bird and examined microscopically. Erythrocyte morphology, including size variation (anisocytosis), shape abnormalities (poikilocytosis), and other abnormalities were evaluated in the monolayer region of the smear. Results: The study found that 44.6% were infected with haemoparasites. Five species of hemoparasites were found during this study, including Plasmodium, Haemoparoteus, Leucocytozoon, Babesia and Microfilaria. In single infection Plasmodium spp was the most prevalent haemoparasite (51.72%) followed by Haemoproteus spp 3.45%, Babesia 3.45% and Microfilaria 1.72%. In mixed infection Plasmodium and Haemoproteus (19 %), Plasmodium and Microfilaria (8.62%), Babesia and Plasmodium (6.90%), Babesia and Haemoproteus (1.72%), Plasmodium and Leukocytozoon 1.72%, in triple infection Babesia, Haemoproteus and Plasmodium (1.72%). Infected birds showed significantly lower haematological parameters, with globulin and creatinine being crucial, while albumin was lower, and the difference between red blood cell diameters was not significant. Conclusion: The study reveals that haematological parameters and biochemical profiles in poultry birds are crucial indicators of their health and offer practical insights for poultry health management.

Comparative study on serum biochemical profiles during different states of reproduction in Jersey crossbred cows

Comparative study on serum biochemical profiles during different states of reproduction in Jersey crossbred cows

Effect of feeding back soldier fly larvae meal on immune response and serum biochemistry profile in broilers

Effect of feeding back soldier fly larvae meal on immune response and serum biochemistry profile in broilers

Efeitos do colírio de cetorolaco de trometamol sobre o hemograma, a bioquímica sérica e a urinálise em cães saudáveis

ABSTRACT This study aimed to evaluate whether the use of trometamol ketorolac for 30 consecutive days may change the blood count, the serum biochemistry profile, and the urinalysis of healthy dogs. Eleven small breed dogs (4.6-10kg), with ages ranging from 1 to 9 years were enrolled in the study. Dogs received 40μL of 0.4% trometamol ketorolac eye drops, every 12 h in both eyes for 30 consecutive days. Blood and urine samples were collected at baseline, and following 15 and 30 days of the beginning of the treatment. Creatinine levels increased significantly at day 15 (1.21±0.1mg/dL) and 30 (1.22±0.1mg/dL) when compared with baseline (0.94±0.1mg/dL) (P&lt;0.01). Significantly increased values of serum potassium were observed only at day 30 (4.66±0.15mEq/L), when compared with day 15 (4.32±0.12mEq/L) and baseline (4.36±0.15mEq/L) (P&lt;0.05). The other hematological and biochemical parameters did not change significantly during the study (P&gt;0.05). From observations of our study, it can be concluded that the instillation of trometamol ketorolac for 30 consecutive days did not cause clinically relevant changes in the blood count, biochemistry profile and in the urinalysis of healthy dogs.

Pathophysiological and pro-inflammatory cytokine surveys on livestock normally infected with Taenia saginata cysticercosis.

Cattle and buffaloes can contract cysticercosis, an infection of the muscles brought on by Taenia saginata larvae. Despite having a global spread, cysticercosis is more prevalent in impoverished nations due to impaired hygiene standards. It has been discovered that Taenia saginata cysticercosis routine visual diagnosis is not very effective, especially in mild infections. Therefore, a more trustworthy in vivo test might be used as an alternative in slaughterhouses and epidemiological studies. Biochemical assays are possibly utilized as an alternative to detect cysticercosis inside a topical environment. Investigating serum biochemical alterations in cattle with cysticercosis was the goal of the current research. As a further method of diagnosis, it was also determined how Cysticercus bovis affected pro-inflammatory cytokines and histopathology. Blood samples from 42 slaughtered cattle (21 healthy and 21 sick animals) were taken from Assiut abattoir. Using an ELISA and spectrophotometer, respectively, their serum's pro-inflammatory cytokines and biochemical profile were evaluated. These cattle were chosen between March 2023 and February 2024. A percentage of 4.6% of the 455 cattle examined after being slaughtered had T. saginata cysticerci infections. All values in the serum biochemistry were considerably different (p < 0.01), whereas the majority of biochemical parameters increased significantly (p < 0.01) in infected animals. In contrast, there was a substantial (p < 0.01) decline in HDL-c, SOD, CAT, and GSH. On the other hand, procytokine inflammatory indices for both TNF-α and IL-1β indicated a substantial increase (p < 0.01) in infected cattle. Additionally, the histological results revealed significant alterations in the tissues of infected livestock. This has been inferred cysticercosis possesses negative impacts on cattle's plasma biochemical profiles, indicating the field applicability of biochemical measures in outbreaks of bovine cysticercosis. Pro-inflammatory cytokine indices and histological changes could be included as further indicators of T. saginata cysticercosis in cattle.

Safety Evaluation of Vatari Guggulu through Acute and 90-day Repeated Dose Toxicity Study in Experimental Animals

Abstract Background: Vatari Guggulu is an Ayurvedic Medicine used in the treatment of Vata disorders. It contains Vatari taila (Castor oil), Shuddha gandhaka (purified Sulphur), Haritaki (Terminalia chebula Retz.), Vibhitaki (Terminalia bellirica [Gaertn.] Roxb.), Amla (Emblica officinalis Gaertn) and guggul (Purified Commiphora mukul Hook). As per the Ayurvedic texts, it is widely used clinically in the treatment of rheumatoid arthritis, hip pain, sciatica, gout, joint pain, and burning sensation. However, there are no systematic toxicity studies were performed to scientifically evaluate the safety of Vatari Guggulu. Objectives: The present study was designed to investigate the safety of Vatari Guggulu in acute and 90-days repeated dose oral toxicity as per Organization for Economic Cooperation and Development guidelines 423 and 408. Materials and Methods: During the acute toxicity, Vatari Guggulu was orally administered at a single limit dose of 2000 mg/kg. The mortality and clinical signs were observed for 14 days. Similarly, the 90-days repeated dose toxicity was studied by administering the Vatari Guggulu at a limit dose of 1000 mg/kg. Results: In the acute toxicity, there were no signs of toxicity, and the LD50 was calculated as &gt;2000 mg/kg. During the 90 days of repeated dose toxicity, the Vatari Guggulu at the dose of 1000 mg/kg did not show any signs of toxicity as compared to the control. The hematological, serum biochemical, and urine analysis profile of the Vatari Guggulu at the dose of 1000 mg/kg did not show any significant difference as compared to the control (P &gt; 0.05). Further, the histopathological data of control and Vatari Guggulu-treated rats were found to be normal. Conclusions: It was concluded that the test drug Vatari Guggulu is found to be safe upto 2000 mg/kg in acute toxicity and 1000 mg/kg in 90-days repeated dose oral toxicity study. The NOAEL of Vatari Guggulu is established as 1000 mg/kg/day.

Effect of dietary Chinese herbal preparation on dry matter intake, milk yield and milk composition, serum biochemistry, hematological profile, and reproductive efficiency of Holstein dairy cows in early postpartum period.

The present study investigated the effects of various inclusion levels of dietary Chinese herbal medicine (CHM) preparation on feed consumption, milk yield and milk composition, serum biochemistry, hematological profile, and reproductive efficiency of Holstein dairy cows. A total of 117 lactating Holstein cows were randomly divided into four groups as control (n = 27; without CHM supplementation) and treatment groups CHM-0.5 (n = 31), CHM-0.75 (n = 29), and CHM-1 (n = 30) fed diet supplemented with 0.5, 0.75, and 1 kg/cow/d for 30 days, respectively. The study began at d 20 postpartum (d 0 of the study). At d 50 postpartum, the cows in all groups were subjected to estrus synchronization using a modified Ovsynch protocol (GPGMH) and observed for reproductive variables. Feed intake, milk yield and milk composition, serum biochemistry and hematological profile, and reproductive efficiency were measured. A significantly higher milk yield with improved milk lactose, milk protein and milk fat were found in the CHM-0.75 group compared to the other groups (p < 0.05). Besides, the estrus response, ovulation rate, ovulatory follicle diameter, and pregnancy rate increased in CHM-0.75 compared to CHM-0 or CHM-0.5 group (p < 0.05). The serum metabolites (glucose, AST, arginine, BUN, and NO) showed variations among the treatment groups at different time points (synchronization, AI, or post-AI). In conclusion, CHM supplementation improves the milk yield, milk composition, and serum metabolites in dairy cows. Daily supplementation of 0.75 kg CHM before the GPGMH protocol application enhances the reproductive traits in dairy cows under summer conditions.

Haematological and serum biochemical profile of bovines infected with infectious bovine rhinotracheitis

Haematological and serum biochemical profile of bovines infected with infectious bovine rhinotracheitis

Proteínas de fase aguda, perfis hematológicos e bioquímicos séricos de cadelas em diestro, mucometra e piometra

ABSTRACT Blood samples from 200 female dogs divided into 6 groups (diestrus, mucometra, pyometra) were evaluated, with the pyometra group categorized according to the ASA classification (American Society of Anesthesiologists), that is, from ASA II to V, totaling six groups. Aiming to analyze the acute phase proteins (APP), hematological and serum biochemical profiles of the female dogs in the study, establishing the differential diagnosis and prognosis according to the group. The SDS-PAGE method was used for protein fractionation, complete blood count using an automated hematological analyzer and histogram, biochemical tests performed using a semi-automatic spectrophotometer and measurement of serum concentrations of sodium and ionic calcium using the selective ion method. The results showed that female dogs with pyometra categorized in the ASA II to V classification revealed biochemical alterations between the study groups, as well as the acute phase proteins, presented variations according to the systemic involvement, degree of inflammatory response and ASA classification of the female dogs with pyometra, in which ceruloplasmin (p=0.07) and transferrin (p=0.07) did not show statistical significance, but showed an increase in their concentration according to the inflammatory evolution, albumin (p&lt;0.0001), IgGCP (p &lt;0.0001), haptoglobin (p&lt;0.0001), alpha acid glycoprotein (p&lt;0.0001), IgGCL (p&lt;0.0001) and the one identified by its atomic weight 23,000 Da (p=0.0031), enabling the assessment of the acute phase response and aiding in the early detection of the systemic inflammatory response and better therapeutic guidance for the patient.

Haematological and serum biochemical indices of growing female pigs fed dietary Spondias mombin leaf meal

Blood serves as physiological indicator of assessing quality of feed offered to an animal, their health status and body functionality. An experiment was carried out to evaluate the haematological and serum biochemical profiles of growing female pigs fed diets with varying levels of Spondias mombin leaf meal (SMLM). Sixteen growing female pigs weighing 10.5 to 14.0 kg were randomly allotted to four treatments supplemented maize feed ingredient with 0.0, 2.5, 5.0 and 7.5% in two phases of feeding with Spondias mombin leaf meal w/w in a Completely Randomized Design with four animals per treatment and replicated twice. Haematological and serum biochemical parameters were assessed. The results show that Packed Cell Volume (PCV), Red Blood Cell (RBC), Platelets and White Blood Cell (WBC) differentials were similar (p&gt;0.05) in mean values, except for Hb which in T4 was significantly (p&lt;0.05) different from other treatments in Hb (p&lt;0.05). There were similarities in mean values of serum biochemical indices across treatments, except albumin and Alkaline Phosphatase (ALP) values in T3 indicating SMLM inclusion improves serum protein synthesis giving rise to more tissue formation. From this study, supplementation of SMLM up to 5.0% maize in growing female pigs’ diet have no deleterious effect on physiological health status and has potential to improve tissue development of animals.

Efficacy of Star Gooseberry (Phyllanthus acidus L.) Feed Additive on the Performance of Broilers with Serum Biochemical Profile

A study was done to find out how star gooseberry (Phyllanthus acidus L.) Fruit juice affected commercial broiler live weight gain, feed consumption, feed conversion ratio, carcass quality, and lipid profiles. A total of 210day-old broiler chicks (Cobb 500) were divided into five dietary treatment groups. The collected data were analyzed by using ANOVA with SPSS version 25. These groups were T0 (control diet), T1 (5 ml/L in water), T2 (7 ml/L in water), T3 (9 ml/L in water), T4 (11 ml/kg in feed), T5 (13 ml/kg in feed), and T6 (15 ml/kg in feed).The T5 group consumed the most feed overall (2829.15±19.85 g), while the T0 group consumed the least feed overall (2767.25±17.55 g). At 5 weeks of age, the T5 group had the highest body weight (2193.3315.18g), followed by the T6 group (2146.00±17.51g), T2 (2088.00±11.15g), T1 (2031.33±20.12 g), and T0 (1871.67± 13.02g), all in that order. The FCR was discovered to be lowest in T5 (1.31) and greatest in T0 (1.51), whereas the FCR of T1, T2, T3, T4, and T6 was 1.41, 1.38, 1.36, 1.35, and 1.32, respectively. The amount of star gooseberry juice has a big impact on the meat's quality. However, there were alterations that were significant (P &lt;0.05) in the carcass weight, breast meat, drumstick meat, wing, shank weight, gizzard, head, liver, lung, skin, thigh bone, and drumstick bone. Only abdominal fat (P &gt; 0.05) was not significant. However, there were significant (P&lt;0.05) changes in total cholesterol, triglycerides, HDL, and LDL. According to the findings, star gooseberry juice can be utilized as a cost-efficient and effective natural feed addition at a rate of 13 ml per kilogram of feed to enhance the broiler chicken's overall performance.

Palmitoleic acid-rich oleaginous yeast Scheffersomyces segobiensis DSM 27193 exerts anti-obesity effects by ameliorating hepatic steatosis and adipose tissue hypertrophy.

Yeast biomass, encompassing fatty acids, terpenoids, vitamins, antioxidants, enzymes, and other bioactive compounds have been extensively utilized in food-related fields. The safety and potential bioactivities of Scheffersomyces segobiensis DSM 27193, an oleaginous yeast strain, are unclear. Scheffersomyces segobiensis DSM 27193 accumulated large palmitoleic acid (POA) levels (43.4 g kg-1 biomass) according to the results of whole-cell components. We annotated the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and predicted the categories and host of the pathogen-host interactions (PHI) genes in S. segobiensis DSM 27193. However, S. segobiensis DSM 27193 did not exert toxic effects in mice. Administration of S. segobiensis DSM 27193 led to substantial weight reduction by diminishing food intake in an obesity mouse model. Additionally, it reversed hepatic steatosis and adipose tissue hypertrophy, and improved abnormalities in serum biochemical profiles such as triglyceride, total cholesterol, low-density lipoprotein cholesterol, lipopolysaccharide, tumor necrosis factor-α, interleukin-1β, and interleukin-6. This study is the first to illustrate the safety and effects of S. segobiensis DSM 27193 against obesity and offers a scientific rationale for its application in functional food supplements. © 2023 Society of Chemical Industry.