Serum Antioxidant Activity Research Articles

The Role of Plant-Based Protein Functional Food in Preventing Acute Respiratory Disease: A Case Study.

The Kaliningrad region is known for its specific climate, which can negatively affect the adaptive potential of the body. This manifests in an increased incidence of respiratory diseases and skin conditions. To prevent high morbidity, a plant protein product was included in the diet of first-year university students. This study aimed to assess the effectiveness of this food intervention in preventing the most common diseases among Kaliningrad students. Two groups of university students took part in the food trial. In the control group, catabolic processes prevailed in nutrient metabolism. Disadaptation manifested itself in the metabolism of proteins, vitamins, minerals, hematopoiesis and humoral immunity. Inflammation was indicated by α1- and α2-globulins, a weak immune response, and IgM and IgG. High oxidative stress and low antioxidative ability of blood serum were observed. The plant-based protein product (FP) helped preserve testosterone level and prevent an increase in catabolic reactions. Moreover, it had a positive effect on both red blood cell hematopoiesis (a smaller increase in the average volume of erythrocytes, the same average concentration and content of hemoglobin, an increased relative red cell distribution width (RDW) and white blood cell hematopoiesis (a beneficial effect for the immune system: lymphocytes, the relative content of neutrophils, monocytes, basophils and eosinophils). The stimulation of humoral immunity was evidenced by beta- and gamma-globulins, an active immune response, the level of IgM and IgG, antioxidant protection, reduction of peroxides and an increase in antioxidant activity of blood serum. The 34-week observation showed a 1.7-fold decrease in the incidence of respiratory illnesses and a 5.7-fold decrease in skin and subcutaneous tissue diseases. Acute respiratory infections were reduced 1.8-fold. There were no cases of community-acquired pneumonia in the treatment group, compared with 55.1‰ in the control group. The incidence of respiratory diseases was 3.3–10.6 times lower in the treatment group than in the control group in weeks 6–19. The findings testify to the prophylactic effect of functional food during social adaptation and acclimatization of students.

Clinical efficiency of antioxidant therapy in patients with liver cirrhosis and acute blood loss

Objective:to determine the clinical efficiency of antioxidant therapy in patients with liver cirrhosis and acute blood loss.Material and methods.The study included 286 patients with liver cirrhosis and acute blood loss of varying degrees of severity. The patients were divided into two series of studies. In the first series, the patients received the therapy according to the clinical diagnostic and treatment protocols, in the second series 1 according to the same protocols in combination with an intravenous administration of the antioxidant complexes “Reamberin” and “Fluimucil”. After 48 hours from admission, the antioxidant status of blood serum, the blebbing index of lymphocytes, the concentration of uric acid, and the level of hemoglobin were analyzed. The indicators of the clinical efficiency of the treatment were determined: risk of absolute mortality, risk of relative mortality, the number needed to treat.Results.The blood serum of the patients from the first series of studies was characterized by pronounced prooxidant activity, high values of the lymphocyte blebbing index, and a small amount of uric acid, which was indicative of the development of oxidative stress. The mortality rate in this series of studies was 26.0 %. The patients of the second series of studies were characterized by pronounced antioxidant activity of blood serum, low values of the lymphocyte blebbing index, and high concentrations of uric acid, which indicated a decrease in the activity of systemic oxidation processes. The mortality rate in this series of studies was 18.2 %. The duration of the treatment decreased from 16.5 days (first series) to 13.0 days (second series).Conclusion.The combined use of the “Reamberin” and “Fluimucil” complexes as part of the therapy according to the clinical diagnostic and treatment protocols leads to a relief of oxidative stress in patients with liver cirrhosis complicated by acute blood loss. The blood serum of these patients is characterized by pronounced antioxidant activity, low values of the lymphocyte blebbing index, and a high concentration of uric acid, which reduces the risk for the development of multiple organ failure syndrome. This therapy decreases the mortality rate to 18.2 % and the duration of the treatment to 13.0 days.

Chitosan vitamin E nanocomposite ameliorates the growth, redox, and immune status of Nile tilapia (Oreochromis niloticus) reared under different stocking densities

A 70-days feeding investigation was undertaken to highlight the influence of dietary incorporation with the conventional form of vitamin E (VE) and chitosan vitamin E nanocomposite (CVEN) on different health aspects of Oreochromis niloticus (O. niloticus) reared under different stocking densities. Growth performance, antioxidant status, and immune response were assessed. Fish (n = 405, average weight: 14.74 ± 0.06 g) were randomly assigned into six groups in triplicates. Three experimental diets were used; 1) basal diet with no additives, 2) basal diet +300 mg/kg VE, 3) basal diet +300 mg/kg CVEN, with two stocking densities for each; standard stocking density (SSD) (15 fish/ aquaria) and high stocking density (HSD) (30 fish/ aquaria). Furthermore, a subsequent challenge with pathogenic Aeromonas sobria (A. sobria) was performed at the end of the feeding period. A significant improvement in the final body weight, total weight gain, feed intake, specific growth rate, and average daily weight gain was found in the CVEN group raised under SSD. Vitamin E supplementation, especially the nano form (CVEN), ameliorates the bad effect of high stocking density on fish growth. Marked elevation in the serum level of vitamin E and growth hormone and decreased AST and ALT levels were observed in the CVEN group reared under SSD compared with other groups. An improvement in the serum and hepatic antioxidant activities and the immunological indicators (lysozyme activity, nitric oxide, phagocytic index, and IL-10) was observed in Vitamin E supplemented groups reared under SSD. Supplementation of vitamin E, especially the nano form, improved the serum and hepatic antioxidant enzymes and decreased lipid peroxidation of fish reared under HSD. Increased survivability of fish reared under HSD by CVEN supplementation. Vitamin E supplementation counteracts the negative impact of HSD on the histoarchitecture of the liver. This study suggested that; supplementation of the fish diet with CVEN (300 mg/kg) could promote the growth performance, antioxidant status, immune response, and increase fish tolerance to rearing under HSD. Moreover, dietary supplementation with CVEN enhanced fish resistance to A. sobria infection.

Promising Antidiabetic and Antimicrobial Agents Based on Fused Pyrimidine Derivatives: Molecular Modeling and Biological Evaluation with Histopathological Effect.

Diabetes is the most common metabolic disorder in both developing and non-developing countries, and a well-recognized global health problem. The WHO anticipates an increase in cases from 171 million in 2000 to 366 million by 2030. In the present study, we focus on the preparation of pyrimidine derivatives as potential antidiabetic and antimicrobial agents. Thein vivoeffect on total serum glucose concentration, cholesterol and antioxidant activity was assessed in adult male albino Wister rats and compared to the reference drug glimperide. Promising results were observed for compound 5. The histopathological study confirms that compound 5 results in significant activity with liver maintenance. The antimicrobial activities were evaluated against several bacterial strains such as Salmonella typhimurium ATCC 25566, Bacillus cereus, Escherichia coli NRRN 3008, Pseudomonas aeruginosa ATCC 10145, Staphylococcus aureus ATCC 6538and fungi such as Rhizopus oligosporus, Mucor miehei and Asperillus niger. Compounds 4 and 5 showed a good inhibition of the bacterial zone compared to the reference drug cephradine. Finally, we suggest protein targets for these drugs based on computational analysis, and infer their activities from their predicted modes of binding using molecular modeling. The molecular modeling for compounds 4 and 5 resulted in improved docking scores and hydrogen bonding. The docking studies are in good agreement with the in vitro and in vivo studies.

Olive Fruit Extracts Supplement Improve Antioxidant Capacity via Altering Colonic Microbiota Composition in Mice.

Oxidative stress, one of the most common biological dysfunctions, is usually associated with pathological conditions and multiple diseases in humans and animals. Chinese olive fruit (Canarium album L.) extracts (OE) are natural plant extracts rich in polyphenols (such as hydroxytyrosol, HT) and with antioxidant, anti-hyperlipidemia, and anti-inflammatory potentials. This study was conducted to investigate the antioxidant capacity of OE supplementation and its related molecular mechanism in mice. Mice (25.46 ± 1.65 g) were treated with 100 mg/kg body weight (BW) OE or saline solution for 4 weeks, and then the antioxidant and anti-inflammatory capacities of mice were examined. The results showed that OE supplement significantly increased the serum antioxidative enzyme activities of total antioxidant activity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase and decreased the serum malondialdehyde (MDA) level, indicating that OE treatment enhanced the antioxidant capacity in mice. qPCR results showed that the transcriptional expression of antioxidant SOD1, CAT, Gpx1, and Gpx2 were significantly down-regulated in the small intestine (jejunum and ileum) after OE administration. Meanwhile, OE treatment significantly decreased the T-AOC and increased the MDA level in the small intestine. Furthermore, OE administration dramatically reduced the mRNA expression of pro-inflammatory cytokines (TNF-α and IL-1β), which confirmed its antioxidant and anti-inflammatory capacities with OE administration. Using amplicon sequencing technology, 16S rRNA sequencing results showed that OE supplement significantly increased the colonic Firmicutes/Bacteroidetes ratio, which also had a negative correlation with the serum MDA level and positively correlated with serum GSH-Px activity through Pearson correlation analysis. Besides that, Alloprevotella was negatively correlated with serum T-AOC. Colidextribacter was positively correlated with serum MDA and negatively correlated with serum T-AOC, SOD, and GSH-Px levels. In summary, this study showed that treatment with 100 mg/kg BW polyphenol-rich OE could alter colonic microbiota community, which was strongly associated with improved antioxidant capacity in mice.

The role of antioxidant system dysfunction in the course of gastroesophageal reflux disease in young patients with autoimmune thyroiditis

Objective — to determine the state of the antioxidant defense system by studying the total antioxidant activity and indicators of the glutathione system in young patients with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT).
 Materials and methods. The study involved 120 patients with GERD and AIT (mean age — 21.9 ± 2.7 уears) and 45 subjects with isolated GERD (mean age — 21.2 ± 2.4 уears). All the investigated patients were the students of higher educational institutions. The general antioxidant activity was investigated as the aggregate indicator of the first phase of antioxidant defense and the state of the glutathione link of the antioxidant system (the activity of glutathione reductase, glutathione peroxidase and reduced glutathione).
 Results. It has been established that in patients with isolated GERD the total antioxidant activity of blood serum was significantly lower in comparison with the control group and was significantly suppressed with the addition of AIT.
 The decreased synthesis of glutathione reductase, glutathione peroxidase and reduced glutathione was reveled at the comorbid pathologies, as well as dependence of these parameters from the morphological forms of the esophageal mucous lesions: the most intensive alterations were observed at the erosive form of the disease.
 Conclusions. The combination of GERD and AIT was accompanied by the changes in the antioxidant defense system parameters. This was manifested in the inhibition of the indicator of the total antioxidant activity and the enzymatic component of the second phase of the system — the glutathione link. These changes were dependent on the morphological form of the esophageal lesions.

Effects of Green Tea and Black Tea Supplementation on Serum Lipids and Antioxidant Enzyme Activity in Rats on a High Fat Diet

본 연구에서 고지방식이를 섭취한 성장기 암컷 쥐를 대상으로 녹차 및 홍차 첨가식이가 혈중 지질 농도와 항산화 효소 활성에 미치는 영향을 분석하였다. 그 결과 체중 증가량은 실험식이에 따른 유의적인 차이가 없었으나 식이효율은 고지방군이 대조군보다 유의적으로 높았다. 혈중 중성지방 농도와 동맥경화지수는 고지방홍차군이 고지방 대조군보다 유의적으로 낮았다. 혈중 총콜레스테롤과 LDL-콜레스테롤 농도는 식이군 간에 차이가 없었고, 혈중 HDL-콜레스테롤 농도는 고지방 대조군이 대조군보다 유의적으로 낮았으나 고지방 녹차군과 고지방 홍차군은 유의적인 차이가 없었다. 간의 총콜레스테롤과 중성지방 함량은 고지방식이군 간에 유의적인 차이가 없었다. 간 조직의 MDA 함량은 고지방 녹차군과 고지방 홍차군이 대조군보다 유의적으로 낮았다. 간 조직의 CAT 활성은 고지방 홍차군이 고지방 대조군에 비하여 유의적으로 높았고, 간 조직의 SOD 활성, GSH-Px 활성은 식이군 간에 유의적인 차이가 없었다. 결론적으로 고지방식이를 섭취한 성장기 암컷 쥐에게 녹차와 홍차를 섭취시켰을 때, 고지방 홍차군은 혈중 중성지방과 동맥경화지수가 고지방 대조군에 비해서 유의적으로 낮았고, 간 조직의 CAT 활성은 유의적으로 높았으며, 간 조직의 MDA 함량은 녹차군과 홍차군이 대조군보다 유의적으로 낮아 차의 섭취는 혈중 지질 개선과 항산화 효소 활성에 유리한 것으로 나타났다.

The Combination of Turmeric (Curcuma domestica) Rhizome Extract and Collagen in A Serum Formulation as an Antioxidant

Turmeric (Curcuma domestica) has nutritious compounds called curcuminoids, which can be used as antioxidants. As an antioxidant, C. domestica extract can be used to ward off free radicals that damage collagen and elastin, a protein that keeps skin moist. This study aimed to determine the antioxidant activity of serum combined with the addition of collagen using the DPPH method. The DPPH was made at a concentration of 80 μg/mL, and the absorption was read at a wavelength of 520 nm using a microplate rider. The study was conducted by making six formulations, namely F0, F1, F2, F3, F4, and F5 obtained the results of serum made from C. domestica extract that can inhibit free radicals and meet the physical evaluation test requirements of serum. Furthermore, the formula is made using only one active ingredient and only collagen to determine the antioxidant activity influenced by the extract or collagen. The results obtained indicate that collagen has a supporting role in adding antioxidant activity apart from the extract used. The highest % inhibition value at F5 with 90.526% can be said to ward off free radicals.

Growth, serum biochemical parameters, salinity tolerance and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss) in response to dietary taurine levels.

This study evaluated the effect of dietary taurine levels on growth, serum biochemical parameters, salinity adaptability, and antioxidant activity of rainbow trout (Oncorhynchus mykiss). Four diets were formulated with taurine supplements at 0, 0.5, 1, and 2% w/v (abbreviated as T0, T0.5, T1, and T2, respectively). Rainbow trouts (initial weight of 80.09 ± 4.72g) were stocked in tanks (180 L capacity), and were fed these diets for six weeks and subsequently underwent salinity acclimation. Physiological indicators were determined before salinity acclimation at 1, 4, 7, and 14days afterwards. Results showed that there were no significant differences in growth performance (final mean weight ranged from 182.35g to 198.48g; percent weight gain was between 127.68% and 147.92%) of rainbow trout in freshwater stage, but dietary taurine supplement significantly increased serum-free taurine content. After entering seawater, the Na+-K+-ATPase activity of T2 group returned to its freshwater levels, and the serum cortisol content was significantly higher than T0 and T0.5 groups. At the end of this experiment, the liver superoxide dismutase activity in the T0 and T0.5 groups was significantly lower than in the T1 and T2 groups, and the liver catalase in the T0 group was the lowest whereas that in the T2 group was the highest. Muscle malondialdehyde content was the highest in the T0 group, and the lowest in the T2 group. Based on the results of this study, supplement of dietary taurine (0.5-2%) enhanced the salinity tolerance in rainbow trout, which increased with the higher taurine concentration.

Effects of Soybean Isoflavone and Astragalus Polysaccharide Mixture on Colostrum Components, Serum Antioxidant, Immune and Hormone Levels of Lactating Sows

Simple SummaryThe reproductive performance of sows plays an important role in the development of the pig industry. The health status during gestation and lactation capacity of sows determines the healthy development and later growth status of piglets. This study aimed to investigate the effects of soybean isoflavone and astragalus polysaccharide mixture on the colostrum components, and serum antioxidant activities, immune function, and hormone levels of lactating sows. Results showed that the soybean isoflavone and astragalus polysaccharide mixture increased the total lactation yield in the lactating phase, and improved the antioxidant, immune and hormone levels in the serum of lactating sows. In conclusion, the soybean isoflavone and astragalus polysaccharide mixture can improve the average daily feed intake, lactation yield, serum antioxidant activity, immune function, and the hormone levels of lactating sows, and the optimum dosage in this study was 200 mg/kg. This study provides a theoretical basis for the application of the soybean isoflavone and astragalus polysaccharide mixture application in the production of lactating sows and a reference basis for the development of green feed additives.The objectives of this study were to investigate the effects of soybean isoflavone (SI) and astragalus polysaccharide (APS) mixture on the colostrum components, serum antioxidant, immune and hormone levels of lactating sows. A total of 72 healthy Yorkshire × Landrace lactating sows, were randomly divided into four treatments with six replicates and three lactating sows for each replicate. The control group was fed the basal diet, while the experimental groups were fed the basal diet with 100, 200 and 300 mg/kg SI and APS mixture in the form of powder, respectively. Compared with the control group, (a) the total lactation yield of the 200 mg/kg group was significantly higher (p < 0.05) at 21 days, (b) there was no significant difference in colostrum composition, (c) TG, CHO and MDA content in each treatment group were significantly decreased (p < 0.05), (d) IgA, GH, IGF-1, TNF-α and SOD contents in the 200 mg/kg group were significantly increased (p < 0.05). The SI and APS mixture could improve the average daily feed intake, lactation yield, serum antioxidant activities, immune function, and hormone levels of lactating sows, and the optimum dosage in this study was 200 mg/kg.

Effects of periodical application of bioactive peptides derived from cottonseed on performance, immunity, total antioxidant activity of serum and intestinal development of broilers

This experiment aimed to examine the effect of periodical application of bioactive peptides derived from cottonseed (BPC) in comparison with using sub-therapeutic doses of lincomycin and the excessive inclusion of vitamin E on performance, immunity, total antioxidant capacity of serum and intestinal morphology of broiler chickens. A total of 240 one-d-old male broiler chicks with similar initial weight (Ross strain) were randomly assigned to 6 groups (8 chicks/pen): non-treated group (basal diet), basal diet supplemented with 2 mg/kg lincomycin, basal diet supplemented with 50 IU vitamin E, basal diet supplemented with 6 g BPC/kg in starter period, basal diet supplemented with 6 g BPC/kg in starter and grower periods and basal diet supplemented with 6 g BPC/kg throughout the whole experiment. The highest final body weight was obtained in the group supplemented with BPC in starter and grower periods. In the finisher phase, broilers fed the diet containing BPC in the starter period and in the whole trial had significantly (P < 0.05) better feed conversion ratios (FCR). Jejunal villus height was significantly elevated in broilers supplemented with antibiotic (P < 0.001), furthermore it tended to be greater in broilers fed BPC in the starter period. The jejunal villus height-to-crypt depth ratio was significantly (P < 0.01) higher in broilers fed the diet containing antibiotic in comparison to other groups. Humoral immune response against Newcastle disease vaccine tended to be elevated in broilers fed the diet containing BPC in the whole trial (P > 0.05). Broilers supplemented with BPC in starter and grower, and in the whole trial had significantly (P < 0.05) higher antibody titers against sheep red blood cells (SRBC). The highest total antioxidant capacity was obtained in broilers supplemented with the excessive level of vitamin E, furthermore it tended to improve in broilers fed the diet containing BPC in the whole trial. In summary, the results of the study indicated that addition of BPC in broiler diets in the whole trial could improve FCR, immune responses and total antioxidant activity of serum, and BPC could be used in broiler diets as an alternative to in-feed antibiotics.

Качество индюшат и безопасность их мяса при трансовариальном использовании глутатиона

Turkey breeding is one of the most stress loaded areas of poultry farming. In order to remedy negative conditions caused by the stress factors associated with artificial incubation, the team of authors suggests transovarian use of a promising and all-pervasive antioxidant in the body – Glutathione. The article presents evidence of the presence of such properties in the claimed tripeptide, which was expressed in a decrease in the products of the peroxide cascade and an increase in the antioxidant activity of blood serum. It is proved that their implementation determines conditions to obtain biologically adequate offspring, promotes an implementation of their productive qualities in further ontogenesis. According to the results of the meat Biosafety study, it is recognized that glutathione does not negatively affect the quality and safety of meat.

CHANGES IN BIOCHEMICAL BLOOD VALUES DURING COMPLEX TREATMENT OF RHINOSINUSITIS POLYPOSA PATIENTS

The aim: To increase the effectiveness of treatment of sinusitis polyposa patients, develop and introduce a new non-traditional complex method of influencing several links of the pathogenesis of this disease, including laser therapy and laser puncture, used in the postoperative period. Materials and methods: In 60 rhinosinusitis polyposa patients the indicators of lipid peroxidation and the state of antioxidant protection were studied. Depending on the type of treatment, the patients were divided into two groups: the first included 30 people who underwent traditional surgical intervention, and the second - 30 people who used complex therapy, including in the postoperative period endonasal laser exposure and laser puncture. Methods: clinical data, the functional state of the nasal mucosa, indicators of lipid peroxidation (hydroperoxide, malondialdehyde) and antioxidant activity (glutathione peroxidase, glutathione reductase, reduced glutathione, superoxide cismutase, catalase) of erythrocyte membranes and blood serum were studied. Results: The proposed complex method for treating lipids of erythrocyte membranes and serum, which includes endonasal surgical intervention, endonasal laser therapy and laser puncture leads to the normalization of physiological functions of the nose, activates antioxidant protection and reduces the peroxide activity of lipids in the membranes of erythrocytes and blood serum. Conclusions: When examining patients in the long-term (after 1 year) period, a significant improvement in 85.7% of cases, an improvement in 10.7%, and absence of effect in 3.6% was achieved. Thus, the proposed method of therapy can be recommended for widespread use in medical institutions.

Liver-targeted delivery of asiatic acid nanostructured lipid carrier for the treatment of liver fibrosis

Liver fibrosis is a major global health concern. Management of chronic liver disease is severely restricted in clinics due to ineffective treatment approaches. However, a lack of targeted therapy may aggravate this condition. Asiatic acid (AA), a pentacyclic triterpenoid acid, can effectively protect the liver from hepatic disorders. However, the pharmaceutical application of AA is limited by low oral bioavailability and poor targeting efficiency. This study synthesized a novel liver-targeting material from PEG-SA, chemically linked to ursodeoxycholic acid (UA), and utilized it to modify AA nanostructured lipid carriers (UP-AA-NLC) with enhanced targeting and improved efficacy. The formulation of UP-AA-NLC was optimized via the Box–Behnken Experimental Design (BBD) and characterized by size, zeta potential, TEM, DSC, and XRD. Furthermore, in vitro antifibrotic activity and proliferation of AA and NLCs were assessed in LX-2 cells. The addition of UP-AA-NLC significantly stimulated the TGF-beta1-induced expression of α-SMA, FN1, and Col I α1. In vivo near-infrared fluorescence imaging and distribution trials in rats demonstrated that UP-AA-NLC could significantly improve oral absorption and liver-targeting efficiency. Oral UP-AA-NLC greatly alleviated carbon tetrachloride-induced liver injury and fibrosis in rats in a dosage-dependent manner, as reflected by serum biochemical parameters (AST, ALT, and ALB), histopathological features (H&E and Masson staining), and antioxidant activity parameters (SOD and MDA). Also, treatment with UP-AA-NLC lowered liver hydroxyproline levels, demonstrating a reduction of collagen accumulation in the fibrotic liver. Collectively, optimized UP-AA-NLC has potential application prospects in liver-targeted therapy and holds great promise as a drug delivery system for treating liver diseases.

Патогенетическое значение мембранных и метаболических нарушений при бронхитах у детей

Study Objective: To study the features of intracellular and extracellular free-radical oxidation (FRO) processes with reference to the cellular membrane condition in various clinical forms of paediatric bronchitis. Study Design: prospective cohort study. Materials and Methods. The study included 237 children aged 2 to 15.5 years old with bronchitis. The patients were divided into three groups depending on the clinical form of bronchitis: acute bronchitis (AB) — 91 children (mean age: 9.8 years old); acute obstructive bronchitis (AOB) — 32 children (mean age: 7.5 years old); and recurrent bronchitis (RB) — 14 children (mean age: 10.2 years old). The control group included 43 conditionally healthy children (mean age: 9.5 years old). We conducted clinical examination, as well as standard instrumental and laboratory tests and interviewed all children. We studied FRO activity (using spontaneous and zymozan-induced luminol-depend chemicoluminescence, ICL), cumulative serum antioxidant activity (AOA), and ceruleoplasmin (CP). Red-cell membranes permeability by sodium was measured using determination of the maximum rate of Na+-Li+-exchange transport (ET) in RBC. Study Results. All patients demonstrated increased rate of Na+-Li+-ET, with the mean value in AB being 232 ± 7.0 (р &lt; 0.05), in OAB — 269 ± 10.0 (р &lt; 0.05), in RB — 328 ± 11.0 μmol Li (р &lt; 0.01). In AB, ICL level was relatively constant vs. normal value irrespective of the disease phase (137.61 ± 22.3 counts/min/1,000 PMNL). In early OAB, ICL was significantly more active (up to 208.98 ± 7.6 counts/min/1,000 PMNL) which normalised later at clinical recovery. In patients with RB, peak ICL was as low as 107.06 ± 26.42 counts/min/1,000 PMNL; at clinical recovery, the value was even lower — 66.41 ± 8.9 counts/min/1,000 PMNL (р &lt; 0.001 vs. healthy children). In acute phase of all forms of the disease, we recorded a significant raise in malondialdehyde; however, its peak concentration was seen in patients with RB; the value was significantly different not only from controls, but also from patients with AB and OAB; and at clinical stabilisation, the value reached 3.72 ± 0.08 μmol (р &lt; 0.05). Patients with AB at recovery had the cumulative AOA of 39.9 ± 0.59% (р &lt; 0.05); however, the control value was not achieved (42.39 ± 0.49%). CP levels were raised as well and reached 64.98 ± 0.43 mg%, but did not differ significantly from the initial value. In OAB, the cumulative OAO did not reach its peak value. Its mean value remained the same. In patients with RB, the cumulative AOA did not reach the normal value, but also reduced a lot (р &lt; 0.05), together with reduced serum CP concentration from 60.20 ± 0.52 mg% to 57.83 ± 0.41 mg% (р &lt; 0.05). Conclusion. Persistence and intensity of membrane and metabolic shifts in children with bronchitis are of great significance in the pathogenesis of a recurrent inflammation in bronchi and can be used as prediction criteria of the disease, allowing to set up a management plan. Keywords: children, bronchitis, free-radical oxidation of lipids, ion exchange system, antioxidant activity, ceruleoplasmin.

Effects of the substitution of oregano essential oil for antibiotics in the plateau-broiler diet

This study was aimed to evaluate the effects of the substitution of oregano essential oil (OEO) for antibiotics in the plateau-broiler diet. Partridge Shank chicken is a broiler breed which has stronger adaptability and disease resistance compared with commercial broiler strain. It is generally slaughtered at 75 -d-old. Here, after a 12-d pre-experiment, 144 57-d-old healthy Partridge Shank chickens were randomly divided into three groups, and fed a basal diet supplemented with OEO (group OEO), a basal diet supplemented with aureomycin (group aureomycin), or a basal diet alone (group control) for 28 days. At the end of the experimental period, production performance, mortality ratio, carcass traits, nutrient digestibility, serum antioxidant performance, intestinal morphology, and intestinal flora structure were determined in each group of broilers. We found that the chickens in group OEO and group aureomycin were improved in average daily gain, average daily feed intake, thigh yield, breast yield, and digestibility of crude protein, reduced in mortality ratio compared with group control (p < 0.05), while there were no significant differences in these indices between these two groups (p > .05). Besides, dietary supplementation with OEO improved serum antioxidant activity and intestinal morphology in the plateau broilers, as well as the richness of beneficial bacteria in the intestine. Thus, OEO improved production performance, nutrient digestibility and reduced mortality ratio of Partridge Shank chickens in the plateau by improving intestinal flora structure. Conclusively, it is practicable that use of OEO to replace growth-promoting antibiotic additives in the plateau-broiler diet. Highlights Oregano essential oil may improved production performance, nutrient digestibility, carcass characteristics and reduced mortality ratio. Dietary supplementation with Oregano essential oil improved the serum antioxidant performance of plateau broilers. Oregano essential oil may improved intestinal flora structure of plateau-broilers.

Effect of Different Levels of Cottonseed Meal Bioactive Peptides on Production Efficiency and Serum Antioxidant Activity of Broiler Chickens

Effect of Different Levels of Cottonseed Meal Bioactive Peptides on Production Efficiency and Serum Antioxidant Activity of Broiler Chickens

PSIX-13 Influence of an integrated fodder additive on productivity and metabolic homeostasis of dairy cattle in transition period

Abstract The transition period is the most stressful for dairy cows in terms of metabolic rate and characterized by dynamic changes in the hormonal profile, loss of appetite, negative energy balance, nutrient and vitamin deficiencies, compromised immunity and oxidative stress. The aim of the research was to balance the increase in cows’ productivity and the consumption of nutrients from the feed during the transition period through the use of energy feed additives (EFS), high protein feed concentrate (PFC) in combination with biologically active (BA) substances. Thirty-six dairy cows of black-motley breed were divided into 3 groups: (1) The main diet (G0, n = 12), not supplemented with additives, (2) additive 1 (G1; supplemented with 0.5 kg EFS / cow per day, n = 12), (3) additive 2 (G2; supplemented with 0.5 kg EFS; 0.5 kg PFC and 0.06 kg BA / cow per day, n = 12). The experimental part of the feeding lasted for 20 days before calving and the first 100 days after calving (DPP). Blood samples were collected during the first and the third months after calving. The biochemical analyzer was used to measure blood serum components. The milk yield was recorded monthly throughout lactation. BCS losses from 7 days prior to calving to 28 DPP were -0.29 for Gr2, -0.42 for Gr1 and -0.60 for Gr0. Feeding with additive 2 (Gr2) increased milk yield (p≤0.05) in the first 100 DPPs and (p≤0.001) 305 DPPs compared to G0. The number of samples with SCC ≥ 500 000 / ml. per 100 DPP was 51% in the Gr0 group, 27% in Gr1, 25% in Gr2. Also, microbiological control showed an excess of Bacterial cells in the milk of cows from group G0, which correlates with SCC indicators. Cows from Gr2had a lower content of ketone bodies (P≥0.001) and higher (P≥0.001) antioxidant activity of blood serum compared to Gr0. Cows from Gr2 had an increase in ALT activity by1.4 times (P &amp;lt;0.05) from the first to the third months after calving.

Blueberry Consumption Challenges Hepatic Mitochondrial Bioenergetics and Elicits Transcriptomics Reprogramming in Healthy Wistar Rats.

An emergent trend of blueberries’ (BB) “prophylactic” consumption, due to their phytochemicals’ richness and well-known health-promoting claims, is widely scaled-up. However, the benefits arising from BB indiscriminate intake remains puzzling based on incongruent preclinical and human data. To provide a more in-depth elucidation and support towards a healthier and safer consumption, we conducted a translation-minded experimental study in healthy Wistar rats that consumed BB in a juice form (25 g/kg body weight (BW)/day; 14 weeks’ protocol). Particular attention was paid to the physiological adaptations succeeding in the gut and liver tissues regarding the acknowledged BB-induced metabolic benefits. Systemically, BB boosted serum antioxidant activity and repressed the circulating levels of 3-hydroxybutyrate (3-HB) ketone bodies and 3-HB/acetoacetate ratio. Moreover, BB elicited increased fecal succinic acid levels without major changes on gut microbiota (GM) composition and gut ultra-structural organization. Remarkably, an accentuated hepatic mitochondrial bioenergetic challenge, ensuing metabolic transcriptomic reprogramming along with a concerted anti-inflammatory pre-conditioning, was clearly detected upon long-term consumption of BB phytochemicals. Altogether, the results disclosed herein portray a quiescent mitochondrial-related metabolomics and hint for a unified adaptive response to this nutritional challenge. The beneficial or noxious consequences arising from this dietary trend should be carefully interpreted and necessarily claims future research.

Evaluation of cottonseed bioactive peptides on growth performance, carcase traits, immunity, total antioxidant activity of serum and intestinal morphology in broiler chickens

The objective of the present experiment was to evaluate the effect of bioactive peptides derived from cottonseed (BPC) on chicken performance, immunity, the total antioxidant activity of serum and intestinal morphology. A total of 280 one-day-old male broiler chicks (Ross 308) were randomly allocated into 1 of the following 7 experimental treatments (5 replicates per treatment with 8 broilers per pen). Five diets were formulated to contain 0 (control), 3, 4, 5 and 6 g BPC/kg of diet in comparison with control + 50 U excessive dietary vitamin E and control + 2 mg lincomycin. At 40 d the BW tended to improve in broilers supplemented with an antibiotic, 3, 5 and 6 g BPC/kg groups (p > .05). In the whole trial, supplementing 5 g BPC/kg increased feed intake of broilers in comparison to other groups (p < .05). In the whole trial broilers fed diets supplemented with 6 g BPC/kg had a significantly better FCR value (p < .001). Supplementation of 3 g BPC/kg increased antibody titres against Newcastle disease virus and sheep red blood cell (p < .01). Dietary supplementation of vitamin E, antibiotic, 3, 4 and 5 g BPC/kg significantly (p < .001) increased total antioxidant activity of serum compared with those fed the basal diet. In conclusion, the results indicated that supplementation of 6 g BPC/kg in broiler diets could induce favourable influences on growth performance, immune responses and total antioxidant activity of serum and it could be used in broiler diets as an alternative to antibiotics. HIGHLIGHTS Broilers fed diets supplemented with 6 g bioactive peptides (BPC)/kg had better FCR. Supplementation of BPC increased humoral immune responses.