Serum ALP Activity Research Articles

Influence of Dietary Supplementation of Condensed Tannins through Leaf Meal Mixture on Intake, Nutrient Utilization and Performance of Haemonchus contortus Infected Sheep.

The study assessed the effect of dietary supplementation of leaf meal mixture (LMM) containing condensed tannins (CT) on feed intake, nutrient utilization and performance of sheep infected with Haemonchus contortus. Eighteen adult sheep of similar age and body weight (25.03±1.52) were included in this study and out of these, 12 sheep were infected with single dose of infective third stage larvae of H. contortus at 2,000 larvae per sheep. The experimental sheep were allocated in three different groups’ i.e. negative control (NC; no infection), control (C; H. contortus infected) and treatment (T; H. contortus infected+CT at 1.5% of the DM through LMM) and the experiment was conducted for a period of 90 d. The intake of dry matter (DM), organic matter (OM) and digestibility of DM, OM, neutral detergent fibre (NDF) and acid detergent fibre (ADF) were comparable among three animal groups. However, digestibility of crude protein (CP) and ether extract (EE) were significantly (p<0.05) higher in NC group as compared to both C and T groups. Nitrogen (N) retention (g/d or % of N intake) was significantly (p = 0.038) lower in C group as compared to T and NC groups. Daily intake (g/kg W0.75) of digestible crude protein (DCP), digestible organic matter (DOM) and total digestible nutrient (TDN) did not differ significantly (p<0.05) in the three groups. Haemoglobin (Hb) and packed cell volume (PCV) were significantly (p<0.001) higher in treatment group as compared to control. The level of Hb and PCV reduced (p<0.001) after 30 days of experimental feeding. CT significantly (p<0.001) reduced serum urea in T group as compared to NC and C groups. Serum proteins differed significantly (p<0.01) among the three groups. The activity of serum enzymes AST, ALT, ALP and LDH were also statistically non significant (p<0.05) among treatments. The weight of abomasal lymph nodes (ALN) in T group was higher (p<0.05) than in C group. Treatment group had lower (p<0.05) total worms and fecal egg count compared to control group. It may be concluded that dietary supplementation of CT through LMM significantly improved the N retention, and inhibited the different developmental stages of Haemonchus contortus in experimental sheep.

うつ血性心不全時における肝障害の臨床的実験的研究

Eighty-nine rabbits weighing about 3Kg were employed. Introduction of talcum powder and slices of sponge into the pericardium of the animals resulted in constrictive pericarditis, which was complicated with congestive heart failure following the intake of salt and the loading with physical exertion. These animals were observed for 112 days postoperatively, as to serum bilirubin, protein fraction, colloidal reactions, ALP, ACP, ChE, GOT, GPT, G6P, ALD, PHI and LDH activities. In the meantime studies were made of enzymatic activities in the serum, liver tissue and myocardium, in reference to histological findings of liver tissue.1) About a week postoperatively there were observed an increase in total bilirubin and in direct bilirubin and a decrease in total protein, about seven weeks postoperatively abnormalities of cobalt reaction were observed.2) Regarding serum enzymatic activities, about a week postoperatively a marked increse was observed in glycolytic enzymes, transaminases especially G6P and LDH. Four-five weeks postoperatively the increase temporarily returned to normal, but again showed a slight increase about 10 weeks postoperatively.3) Enzymatic activities in the liver and the myocardium decreased about a week postoperatively, reaching nearly a normal range about four weeks postoperatively followed by a decrease again about ten weeks postoperatively. Thus they showed a mirror image relationship with serum enzymatic activities.4) Liver tissue findings showed acute liver congestion about a week postoperatively, revealing an increasing cellular vacuolization about four weeks postoperatively, followed by a proliferation of the connective tissue of the central lobule in some cases 16 weeks postoperatively.