Introduction and purpose of the study: The overall prevalence of HCV among adults in sub-Saharan Africa has increased from 3.82 to 3.94% (3). We find a disproportionate prevalence of viral hepatitis C in blood donors in several national blood transfusion centers in Sub-Saharan Africa and the aim of our work was to study the seroprevalence of hepatitis C in blood donors from the center national blood transfusion center of Bamako Methods: This was a descriptive cross-sectional study that took place at the CNTS national blood transfusion center in Bamako from July to December 2019. Our study concerned blood donors (volunteers and relatives) presenting to the CNTS in Bamako during the study period. It focused on an exhaustive random sample of CNTS blood donors, the variables studied were gender, marital status, type of donor HCV+, HCV-, age and number of donations. Serodiagnosis was determined by the ELISA technique. Results: We collected 250 samples, the 26-35 age group was the most represented with 49.6%; the prevalence of hepatitis C was 1%. Hepatitis C marker positivity has been observed in family donors. Conclusion: The seroprevalence of hepatitis C during our study was 1%. The ELISA technique with monolisa HCV reagent was used as the method. We found a relationship between seroprevalence and few sociodemographic aspects.