Serial Broth Dilution Method Research Articles

A microfluidic device for antimicrobial susceptibility testing based on a broth dilution method

Bacterial resistance to antimicrobial compounds is increasing at a faster rate than the development of new antibiotics; this represents a critical challenge for clinicians worldwide. Normally, the minimum inhibitory concentration of an antibiotic, the dosage at which bacterial growth is thwarted, provides an effective quantitative measure for antimicrobial susceptibility testing, and determination of minimum inhibitory concentration is conventionally performed by either a serial broth dilution method or with the commercially available Etest® (Biomerieux, France) kit. However, these techniques are relatively labor-intensive and require a significant amount of training. In order to reduce human error and increase operation simplicity, a simple microfluidic device that can perform antimicrobial susceptibility testing automatically via a broth dilution method to accurately determine the minimum inhibitory concentration was developed herein. As a proof of concept, wild-type (ATCC 29212) and vancomycin-resistant Enterococcus cells were incubated at five different vancomycin concentrations on-chip, and the sample injection, transport, and mixing processes occurred within five reaction chambers and three reagent chambers via the chip's automatic dispensation and dilution functions within nine minutes. The minimum inhibitory concentration values measured after 24h of antibiotic incubation were similar to those calculated using Etest®. With its high flexibility, reliability, and portability, the developed microfluidic device provides a simple method for antimicrobial susceptibility testing in an automated format that could be implemented for clinical and point-of-care applications.

Imidazolium salts with antifungal potential for the control of head blight of wheat caused by Fusarium graminearum.

Evaluate the invitro effect of imidazolium salts (IMS) on the conidia germination and mycelial growth of Fusarium graminearum and their invivo efficacy for suppressing the symptoms of the disease and infection of kernels in wheat plants. The minimum inhibitory concentrations (MIC) of three IMS (C16 MImCl, C16 MImMeS and C16 MImNTf2 ) were determined for four F.graminearum isolates using serial broth dilution method. The MICs found for all IMS were either 3·12 or 6·25μgml(-1) across the isolates, with the former as the most frequent. In the mycelial growth assay on potato dextrose agar media, only the C16 MImCl among the IMS reduced 50% of mycelial growth of one isolate at an estimated concentration of 0·32mgml(-1) . The time-kill curves showed a strong fungicidal effect starting 1h after incubation at a concentration of 12·5μgml(-1) , representing a fourfold increase in the most frequent MIC. The C16 MImCl sprayed onto the spikes of potted wheat plants during the flowering stage reduced disease intensity at levels comparable to the commercial fungicide when applied preventatively (1h prior to fungal inoculation), rather than curatively, and at the higher dosage (2mgml(-1) ) rather than lower dosage (0·5mgml(-1) ). C16 MImCl proved to be a potent inhibitor of F.graminearum growth and provided good levels of control of the disease at levels comparable to a commercial fungicide, in wheat plants treated prior to fungal infection during flowering stages. This study suggests the potential of using IMS as alternative to the hazardous standard fungicides in the management of Fusarium head blight of wheat.

Synthesis, characterization and antimicrobial screening of thiazole based 1,3,4-oxadiazoles heterocycles

In an effort to find a new pharmacologically dynamic molecule, we report here the synthesis and in vitro antimicrobial activity of various N-(4-methyl-5-(4-((substituted phenylamino) methyl)-5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)thiazol-2-yl) benzamides. The structures of newly synthesized compounds were assigned on the bases of IR, 1H NMR, 13C NMR, and mass spectral data. Synthesized compounds were screened for their antibacterial and antifungal activities on gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and three fungi (Candida albicans, Aspergillus niger and Aspergillus clavatus) by serial broth dilution method. Compounds 5e and 5k were associated with considerable potential antibacterial and compounds 5a and 5b antifungal activities.

Design, synthesis and antimicrobial activity of 6-N-substituted chitosan derivatives

Three novel 6-N-substituted chitosan derivatives were designed and synthesised and characterized by FTIR and NMR. The degree of substitution was calculated by elemental analysis results. The antimicrobial activities of the target compounds were evaluated by twofold serial broth dilution method and poisoned food technique. The antifungal activities of 6-aminoethylamino-6-deoxy chitosan (3), 6-butylamino-6-deoxy chitosan (4) and 6-pyridyl-6-deoxy chitosan (5) were significantly increased against Rhizoctonia cerealis, Fusarium oxysporum and Botrytis cinerea, and the inhibition rate ranged from 22.48% to 63.56% at the concentration of 0.2mg/mL. The compound 3 had better antibacterial activities than chitosan, and the minimum inhibition concentration of which ranged between 6.25 and 25mg/L against gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis and Bacillus anthracis) and gram-negative bacteria (Escherichia coli, Salmonella typhi). The antibacterial activities of 6-N-substituted chitosan tended to increase with the increase of the number of –NH2 group.

A Study on Antitubercular and Antimicrobial Activity of Isoniazid Derivative

Background:: Hydrazones belonging to azomethine class of compounds have attracted the attention of many chemists owing to their wide spectrum of pharmacological activity profile with structural flexibility and ligating behavior. Objectives:: We aimed to synthesize a potential ligand containing both, isonicotinoylhydrazide and 3-ethoxy-2-hydroxybenzilidine moieties, linked through azomethine group. Materials and Methods:: In this descriptive study, a new ligand derived by the condensation of isonicotinoylhydrazide and 3-ethoxysalicylaldehyde have been synthesized. Ligand was characterized on the basis of various spectroscopic techniques like IR, 1H and 13C NMR studies, elemental analysis. The compound was subjected to antimicrobial and anti-tubercular activity screening using serial broth dilution method and minimum inhibitory concentration (MIC) is determined. The ligand was evaluated for its antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus ATCC 9144) and Gram-negative bacterium (Escherichia coli ATCC 11303). New compound synthesized showed good biological activity against tested bacteria. Furthermore, ligand shows high levels of activity against Mycobacterium tuberculosis (H37RV) in vitro. Results:: The elemental analysis, 1H and 13C NMR studies of ligand confirm the formation of title compound with the molecular formula C15H15N3O3. Ligand shows inhibition against mycobacterium at concentration 4 µg/mL. Conclusions:: The schiff bases ligand was readily prepared for evaluation against M. tuberculosis in good yield. Compound show good activity in vitro.

Detection of antimicrobial activity of banana peel (Musa paradisiaca L.) on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study.

Introduction and Aim:Banana is used widely because of its nutritional values. In past, there are studies that show banana plant parts, and their fruits can be used to treat the human diseases. Banana peel is a part of banana fruit that also has the antibacterial activity against microorganisms but has not been studied extensively. Since, there are no studies that relate the antibacterial activity of banana peel against periodontal pathogens. Hence, the aim of this study is to determine the antimicrobial activity of banana peel extract on Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans).Material and Methods:Standard strains of P. gingivalis and A. actinomycetemcomitans were used in this study which was obtained from the in-house bacterial bank of Department of Molecular Biology and Immunology at Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre. The banana peel extract was prepared, and the antibacterial activity was assessed using well agar diffusion method and minimum inhibitory concentration was assessed using serial broth dilution method.Results:In the current study, both the tested microorganisms showed antibacterial activity. In well diffusion method, P. gingivalis and A. actinomycetemcomitans showed 15 mm and 12 mm inhibition zone against an alcoholic extract of banana peel, respectively. In serial broth dilution method P. gingivalis and A. actinomycetemcomitans were sensitive until 31.25 μg/ml dilutions.Conclusion:From results of the study, it is suggested that an alcoholic extract of banana peel has antimicrobial activity against P. gingivalis and A. actinomycetemcomitans.

A microwave-assisted facile synthesis of novel coumarin derivatives containing cyanopyridine and furan as antimicrobial agents

In this paper, we have described the microwave-assisted method for the synthesis of novel 4-(substitutedphenyl)-2-(furan-2-ylmethyleneamino)-6-(2-oxo-2H-chromen-3-yl)nicotinonitriles (5a–m). Compound (I) 3-acetyl-2H-chromen-2-one was reacted with aromatic aldehydes, malononitrile and ammonium acetate in microwave conditions afforded compounds 3a–m, which on further microwave irradiation at 300W for 8–10min in the presence of trace amount of ZnCl2 furnished 5a–m. Structures of synthesized compounds have been confirmed by IR, 1H NMR, 13C NMR and mass spectra. Antimicrobial activity of the compounds was studied against several bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes) and fungi (Candida albicans, Aspergillus niger, Aspergillus clavatus) by using the serial broth dilution method. Five compounds 5d, 5f, 5j, 5k and 5l were found to possess high activity comparable to ampicillin at 50μg/ml against E. coli and P. aeruginosa.

Effect of aqueous and alcoholic Stevia (Stevia rebaudiana) extracts against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine: An in vitro study.

Introduction:Stevia (S. rebaudiana) a herb which has medicinal value and was used in ancient times as a remedy for a great diversity of ailments and sweetener. Leaves of Stevia contain a high concentration of Stevioside and Rebaudioside which are supposed to be sweetening agents.Aim:To compare the efficacy of aqueous and alcoholic S. rebaudiana extract against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine.Materials and Methods:In the first part of the study, various concentrations of aqueous and ethanolic Stevia extract were prepared in the laboratory of Pharmacy College. It was then subjected to microbiological assay to determine its zone of inhibition using Agar disk diffusion test and minimum inhibitory concentration (MIC) using serial broth dilution method against Streptococcus mutans and Lactobacillus acidophilus. Chlorhexidine was used as a positive control. One way Analysis of Variance (ANOVA) test was used for multiple group comparisons followed by Tukey post hoc for group wise comparisons.Results:Minimum inhibitory concentration (MIC) of aqueous and ethnolic Stevia extract against Streptococcus mutans and Lactobacillus acidophilus were 25% and 12.5% respectively. Mean zone of inhibition of the aqueous and alcoholic Stevia extracts against Streptococcus mutans at 48 hours were 22.8 mm and 26.7 mm respectively. Mean zone of inhibition of the aqueous and alcoholic Stevia extracts against Lactobacillus acidophilus at 48 hours were 14.4 mm and 15.1 mm respectively. Mean zone of inhibition of the chlorhexidine against Streptococcus mutans and Lactobacillus acidophilus at 48 hours was 20.5 and 13.2 respectively.Conclusion:The inhibitory effect shown by alcoholic Stevia extract against Streptococcus mutans and Lactobacillus acidophilus was superior when compared with that of aqueous form and was inferior when compared with Chlorhexidine.

Anticariogenic activity and phytochemical studies of crude extract from some Indian plant leaves

Aim:The aim was to screen the selected Indian plants for their antibacterial efficacy against four cariogenic bacteria Lactobacillus acidophilus (LA)(Microbial Type Culture Collection [MTCC]-*447), Lactobacillus casei (LC) (MTCC-1423), Streptococcus mutans (SMU) (MTCC-890) and Staphylococcus aureus (MTCC-96). To identify and characterize active principle present in these plants for the treatment of dental caries.Materials and Methods:The dried plant leaves materials are extracted by cold extraction using hexane, ethyl acetate, methanol, and distilled water. The solvents were evaporated, and the dried masses were suspended in dimethyl sulfoxide and used for anticariogenic activity by agar well diffusion method. Minimum inhibitory concentration (MIC) was evaluated by two-fold serial broth dilution method. Preliminary phytochemical analysis of effective extract was carried out by thin-layer chromatography (TLC) and bioautography.Results:Ethyl acetate and hexane extract of Eucalyptus globules was found most effective against L. acidophilus with MIC value 31 μg/ml and 62 μg/ml, respectively. Ethyl acetate extracts of Acacia nilotica and methanolic extract of E. globules also exhibited antibacterial activity against SMU and L. casei with MIC value of 50 μg/ml. Qualitative analysis of E. globules revealed the presence of alkaloids, terpenoids, phenolic compounds, and cardiac glycosides. The active principle responsible for the anticariogenic activity from E. globules were separated by TLC and subjected to bioautography using SMU, LA and LC.Conclusion:Anticariogenic activity and preliminary phytochemical analysis revealed that E. globule have potential to treat dental caries.

An efficient synthesis, characterization, and antimicrobial screening of tetrahydropyrimidine derivatives

Several new substituted 1,2,3,4-tetrahydropyrimidine derivatives 2a–h have been synthesized by the modified Biginelli and Hantzsch reactions and compounds were characterized by spectral techniques. All compounds were evaluated for their in vitro antimicrobial activity against different strains of Gram-negative (E. coli and P. aeruginosa) and Gram-positive (S. aureus and S. pyogenus) bacteria and selected fungi C. albicans, A. niger, and A. clavatus using serial Broth dilution method (Mueller–Hinton broth dilution method). Compound 2e was found active against both Gram-negative and -positive bacterial strains used for present study, while compound 2b was good active against Gram-negative and S. pyogenus bacterial strains. Compounds 2a–h were not that much significant against (C. albicans, A. niger, and A. clavatus) selected fungal strains.

Synthesis and characterization of novel benzimidazole bearing pyrazoline derivatives as potential antimicrobial agents

A new series of compounds N-(4-(2-(3-(1H-benzo[d]imidazol-2-yl)-5-(aryl)-4,5-dihydro-1H-pyrazol-1-yl)-2-oxoethoxy)phenyl)acetamides (5a–u) were synthesized and structures of these compounds were elucidated by spectral (IR, 1H NMR, 13C NMR, and mass spectra) analysis. Antimicrobial activity was measured against Escherichia coli (MTCC 443), Pseudomonas aeruginosa (MTCC 1688), Staphylococcus aureus (MTCC 96), Streptococcus pyogenes (MTCC 442), Candida albicans (MTCC 227), Aspergillus niger (MTCC 282), and Aspergillus clavatus (MTCC 1323) by serial broth dilution method. Evaluation of antimicrobial activity revealed that compounds 5f, 5i, 5q, and 5t were the most active antibacterial, while compounds 5e, 5g, 5h, 5j, 5p, 5r, and 5u were the most potent antifungal agents as compared to standard drugs and thus could be promising new lead molecules.

In vitro fungitoxic and cytotoxic efficacy of Chloroxylon swietenia DC. leaf essential oil

Essential oil of Chloroxylon swietenia was studied for its antifungal potential against clinically isolated fungal strains as well as the cytotoxic effect on Vero cells. The essential oil was analyzed by gas chromatography (GC) and GC–mass spectrometry (GC–MS). Antifungal activity was studied by disc diffusion method and serial broth dilution method. The toxicity of the essential oil was evaluated in normal Vero cell line by MTT assay. The GC and GC–MS analyses revealed that the essential oil contained 36 compounds and the major chemical compounds were germacrene-D (22.4%) followed by limonene (17.3%) and geijerene (15.4%). The antifungal assay showed that Candida albicans was the most sensitive fungal strain tested, with the strongest mean inhibition zone (22.0±1.32 mm) followed by C. parapsilosis (21.5±1.32 mm). The results of the minimum inhibitory concentration (MIC) indicated that the oil inhibited all the fungal strains tested at the highest concentration of 125 μg/mL. Toxicity on normal Vero cell line showed the IC50 value of 115.47 μg/mL. The results obtained in the present study will certainly be helpful in future clinical trials to validate its use as a phytotherapeutic product.

A new hybrid approach and in vitro antimicrobial evaluation of novel 4(3H)-quinazolinones and thiazolidinone motifs

We have synthesized some novel fluorine containing 5-arylidene derivatives bearing different pharmacophores and heterocyclic systems like quinazolinone along with 4-thiazolidinone, which may act as potential antimicrobial agents. They have been synthesized by the Knoevenagel condensation of N-(2-(4-fluorophenyl)-4-oxothiazolidin-3-yl)-4-(4-oxo-2-p-tolylquinazolin-3(4H)-yl)benzamide with aromatic aldehydes to form N-(5-(aryl)-2-(4-fluorophenyl)-4-oxothiazolidin-3-yl)-4-(4-oxo-2-p-tolylquinazolin-3(4H)-yl)benzamides (6a–t). The products were characterized by IR, 1H, 13C and 19F NMR and mass spectral techniques. The synthesized compounds were evaluated for their in vitro antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus clavatus) using serial broth dilution method. Compounds 6b, 6d, 6i, 6j, 6k, 6n and 6s were the most noteworthy derivatives identified in the present study because of their remarkable in vitro antimicrobial potency.

Synthesis, characterization and biological evaluation of 4-oxo-thiazolidine compounds

A series of compounds 4-oxo-thiazolidine derivatives AJ5a–j were synthesized by condensation reactions. The structure of synthesized compounds was characterized by IR, 1H NMR, 13C NMR and mass spectroscopy. The present paper deals with newly synthesized thiazolidine compounds AJ5a–AJ5j that were screened for their antimicrobial activity against different strains of bacteria and fungi using serial broth dilution method (Mueller–Hinton broth dilution method). In vitro antitubercular activity of compounds AJ5a–j was carried out against Mycobacterium tuberculosis H37Rv. Compounds AJ5a, AJ5d, AJ5e, AJ5f and AJ5g were found most active against selected bacterial strains (MIC=62.5μg/mL) and compounds AJ5a–j were found moderately active against M. tuberculosis.

Synthesis, characterization and antimicrobial screening of hybrid molecules containing quinoline, pyrimidine and morpholine analogues

In an attempt to find new bio-active molecules, a series of compounds N-(4-(2-chloroquinolin-3-yl)-6-(aryl)pyrimidin-2-yl)-2-morpholinoacetamides (5a–l) were synthesized by multistep reactions. Compounds were characterized by IR, NMR and mass spectra. Antimicrobial screening of title compounds (5a–l) was carried out against Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, Aspergillus clavatus) using serial broth dilution method. On the basis of statistical analysis, it is observed that these compounds gave significant co-relation. Newly synthesized compounds 5e, 5f, 5g, 5i and 5l showed significant potency against different microbial strains. A novel series of compounds N-(4-(2-chloroquinolin-3-yl)-6-(aryl)pyrimidin-2-yl)-2-morpholinoacetamides (5a–l) were synthesized by multi-step reactions. Newly synthesized compounds were characterized by various spectroscopic techniques and screened for in vitro antimicrobial activity.

Synthesis and Characterization of Clubbed Thiazolidin-4-one, Quinoline and Barbitone Derivatives as a Potent Antimicrobial Agents

In the present investigation, it was of interest to synthesized some new derivatives of pyrimidine- 2,4,6(1H,3H,5H)-trione moiety incorporated with different biologically active pharmacophores such as thiazolidin-4- one, barbitone and 2-chloroquinoline-3-carbaldehyde. The structures of synthesized compounds were elucidated by IR, 1H NMR, 13C NMR, and mass spectral data. Antimicrobial screening of title compounds were examined against Grampositive bacteria (Staphylococcus aureus and Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and three fungal species (Candida albicans, Aspergillus niger, and Aspergillus clavatus) by serial broth dilution method. Compounds 6b, 6e, 6f and 6h were associated with considerably higher antibacterial and antifungal activities. Keywords: Barbitone, Quinoline, Thiazolidin-4-one

Assessment of antimicrobial potential of 10% ginger extract against Streptococcus mutans, Candida albicans, and Enterococcus faecalis: an in vitro study.

Streptococcus mutans, Candida albicans, and Enterococcus faecalis are the three oral microorganisms most commonly implicated in the causation of oral infections. All these oral microorganisms have shown resistant to routinely used antimicrobials. There is a need for an antimicrobial agent which is effective, safe, and economical. Zingiber officinale, commonly known as ginger is one such plant product which has been used from ancient time. It has been shown to possess promising inhibitory effect on many of the oral microorganisms. On review of dental literature, there was scarcity of studies which had tried to assess antimicrobial potential of ginger extract against S. mutans, E. faecalis, and C. albicans; hence, the present study was designed. To evaluate the in vitro antimicrobial potential of 10% ginger extract against S. mutans, E. faecalis, and C. albicans. Laboratory setting and experimental design. In the first part of the study, 10% ethanolic ginger extract was prepared in the laboratory of Pharmacy College. It was then subjected to microbiological assay to determine its zone of inhibition using Agar disk diffusion test and minimum inhibitory concentration (MIC) using serial broth dilution method against S. mutans, C. albicans, and E. faecalis. 10% ethanolic ginger extract showed: (a) Maximum zone of inhibition of 8 mm, 14 mm, and 11 mm against S. mutans, C. albicans, and E. faecalis respectively. (b) MIC of 1.25%, 2.5%, and 2.5% against S. mutans, C. albicans, and E. faecalis respectively. 10% ethanolic ginger extract was found to possess antimicrobial potential against all the three pathogens used in the study.

Synthesis and study of 1,3,5-triazine based thiazole derivatives as antimicrobial agents

A series of novel compounds N′-(4-(arylamino)-6-(thiazol-2-ylamino)-1,3,5-triazin-2-yl)isonicotinohydrazides (3a−l) were synthesized by a series of multistep reactions. Newly synthesized compounds have been characterized by IR, 1H NMR, 13C NMR and mass spectral data. Antimicrobial screening of title compounds (3a−l) was examined against Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and three fungi (Candida albicans, Aspergillus niger, Aspergillus clavatus) by using serial broth dilution method. Synthesized compounds showed potent inhibitory action against test organisms. Screened compounds 3k and 3l were associated with considerably higher antibacterial and antifungal activities than commercially used antibiotics.

Microwave induced synthesis of fluorobenzamides containing thiazole and thiazolidine as promising antimicrobial analogs

New 5-arylidene derivatives, which bear a fluorine atom in the 4th position of the benzoyl group as starting compound, have been synthesized by the condensation of 4-fluoro-N-(4-methyl-5-(2-(4-oxo-3-phenylthiazolidin-2-ylidene)hydrazinecarbonyl)thiazol-2-yl)benzamide and Knoevenagel condensation with aromatic aldehydes to form N-(5-(2-(5-(arylidene)-4-oxo-3-phenylthiazolidin-2-ylidene)hydrazinecarbonyl)-4-methylthiazol-2-yl)-4-fluorobenzamides (6a–o) through conventional and microwave methods. The structures of synthesized compounds were established by spectroscopic as well as spectrometric techniques (IR, 1H NMR, 13C NMR, 19F NMR spectroscopy and mass spectrometry). Antimicrobial screening of 5-arylidene derivatives 6a–o was done against Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus clavatus) using serial broth dilution method. Compounds 6g and 6j were found to be most active at MIC 12.5μg/mL against selected bacterial strains and compound 6e was found to be most active at MIC 25μg/mL against selected fungal strains. It has been further observed that the presence of fluorine atom at the 4th position of the benzoyl group in the final compounds is essential for enhancing the antimicrobial activity.

Synthesis of promising antimicrobial agents: a novel series of N-(4-(2,6-dichloroquinolin-3-yl)-6-(aryl)pyrimidin-2-yl)-2-morpholinoacetamides

A series of novel compounds N-(4-(2,6-dichloroquinolin-3-yl)-6-(aryl)pyrimidin-2-yl)-2-morpholinoacetamides (5a–l) were synthesized by a series of multistep reactions. Newly synthesized compounds have been characterized by IR, 1H NMR, 13C NMR, and mass spectral data. Antimicrobial screening of title compounds 5a–l was examined against Gram-positive bacteria (Staphylococcus aureus and Streptococcus pyogenes), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and three fungi (Candida albicans, Aspergillus niger, and Aspergillus clavatus) by serial broth dilution method. Synthesized compounds showed potent inhibitory action against test organisms. Screened compounds 5e–g and 5i–l were associated with considerably higher antibacterial and antifungal activities than commercially used antibiotics.