The Big Five Inventory-2 (BFI-2), as a comprehensive measure encompassing the Big Five domains and their facets, has undergone numerous validations in various languages. To validate a Serbian adaptation of the BFI-2, data from two samples, comprising a total of 1016 adult participants, along with a US sample (N = 1000), were utilized. The originally proposed factor structure of Serbian adaptation of the BFI-2 was confirmed, along with partial scalar invariance across Serbian and US samples. Convergent validity was established in relation to an alternative Big Five measure, as well as six-factor and seven-factor models. Criterion validity was confirmed through correlations with various behavior, cognitive, and emotional indicators. These findings contribute to evidence supporting the cross-cultural validity of BFI-2.