The supply of soil nitrogen to successive wheat crops was compared for direct-drilled and conventional tillage systems on a red earth in south-eastern Australia. There was no significant effect of tillage on the total plant uptake of nitrogen, nor on the decline of soil nitrogen during a 5-year cropping cycle. During the growth of the last two crops in the cycle, the rates of net mineralization, nitrate leaching from the topsoil and crop-nitrogen uptake were measured, for both tillage systems, by an in situ sequential sampling method. For the first of these crops the rates of net mineralization were unaffected by tillage, but for the second crop the rates were greater on the direct-drilled soil. Variation in the rates of mineralization within both seasons could largely be explained by changes in temperature and soil water content. Leaching of nitrate from the topsoil was consistently greater for the direct-drilled soil, where seedlings initially accumulated less nitrogen than those growing on conventionally-cultivated soil. However, the differences in uptake did not persist, and at maturity similar amounts of nitrogen, representing less than 50% of the seasonal net mineralization, were accumulated in the above-ground parts of crops grown under the two systems.