Sequencing Batch Reactor Research Articles

The Study On The Effects Of Micropollutants Removal By 2 Stage PAC-AGS Process

In this study, a lab-scale sequencing batch reactor of effective volume 3.5 L was used to evalute the performance of mircopollutants removal by 2 Stage PAC-AGS process. It consisted of raw water tank, 1<sup>st</sup> AGS reactor, 2<sup>nd</sup> PAC-AGS reactor, reservoir, effluent tank. AGS has superior precipitation of SVI<sub>30</sub> 58 mL/g and high EPS concentration. For combination AGS with PAC, PAC was evenly distributed by attaching to AGS surface. The result of operating, BOD, TOC, SS, T-N, T-P of 2<sup>nd</sup> PAC-AGS effluent was 1.2 mg/L, 6.0 mg/L, 1.9 mg/L, 15.5 mg/L, 0.13 mg/L. SUVA of influent, 1<sup>st</sup> AGS effluent, 2<sup>nd</sup> PAC-AGS effluent was 0.97 L/mg/m<sup>-1</sup>, 1.30 L/mg/m<sup>-1</sup>, 2.03 L/mg/m<sup>-1</sup> respectively. It means that hydrophilic and small-molecule compounds was mainly removed of DOC. The 14 species of micropollutants was detected in influent from A STP. Micopollutants concentration of influent, effluent of bioreactor from A STP was 68,016.3 ng/L, 710.4 ng/L respectively and the micopollutants concentration of effluent from 2 Stage PAC-AGS process was 18.1 ng/L. It shows that 2 Stage PAC-AGS process has superior performance for micropollutants removal than bioreactor from A STP in operation. Specially, Diclofenac acid, Carbamazepine were known for high RQ(Risk Quotient) was not detected in effluent from 2 Stage PAC-AGS process.

The synthesis of ectoine enhance the assimilation of ammonia nitrogen in hypersaline wastewater by the salt-tolerant assimilation bacteria sludge

In contrast to nitrification-denitrification microorganisms that convert ammonia nitrogen in hypersaline wastewater into nitrogen for discharge, this research utilizes sludge enriched with salt-tolerant assimilation bacteria (STAB) to assimilate organic matter and ammonia nitrogen in hypersaline wastewater into ectoine - a biomass with high economic value and resistance to external osmotic pressure. The study investigates the relationship between the synthesis of ectoine and nitrogen removal efficiency of STAB sludge in three sequencing batch reactors (SBR) operated at different salinities (50, 75, and 100 g/L) and organic matter concentrations. The research reveals that, under low concentration carbon sources (TOC/N = 4, NH4+-N = 60 mg/L), the ammonia nitrogen removal efficiency of SBR reactors increased by 14.51 % and 17.25 % within 5 d and 2 d, respectively, when salinity increased from 50 g/L to 75 g/L and 100 g/L. Under high concentration carbon sources (TOC/N = 8, NH4+-N = 60 mg/L), the ammonia nitrogen removal efficiency of STAB sludge in the three reactors stabilized at 80.20 %, 76.71 %, and 72.87 %, and the total nitrogen removal efficiency was finally stabilized at 80.47 %, 73.15 %, and 65.53 %, respectively. The nitrogen removal performance by ammonium-assimilating of STAB sludge is more sustainable under low salinity, while it is more short-term explosive under high salinity. Moreover, the intracellular ectoine concentration of STAB sludge was found to be related to this behavior. Empirical formulas confirm that STAB sludge synthesizes ectoine from nutrients in wastewater through assimilation, and intracellular ectoine has a threshold defect (150 mg/gVss). The ectoine metabolism pathways of STAB sludge was constructed using the Kyoto Encyclopedia of Genes and Genomes (KEGG). The ammonia nitrogen in sewage is converted into glutamic acid under the action of assimilation genes. It then undergoes a tricarboxylic acid cycle to synthesize the crucial precursor of ectoine - aspartic acid. Subsequently, ectoine is produced through ectoine synthase. The findings suggest that when the synthesis of intracellular ectoine reaches saturation, it inhibits the continuous nitrogen removal performance of STAB sludge under high salinity. STAB sludge does not actively release ectoine through channels under stable external osmotic pressure.

Efficient nitrogen removal from real municipal wastewater and mature landfill leachate using partial nitrification-simultaneous anammox and partial denitrification process

Anaerobic ammonia oxidation (anammox) of municipal wastewater is a research focus, especially the combined treatment with mature landfill leachate is a current research hotspot. In this study, municipal wastewater was treated by partial nitrification via sequencing batch reactor (SBR), and its effluent and mature landfill leachate were then mixed into an up-flow anaerobic sludge blanket (UASB) for simultaneous anammox and partial denitrification reaction. Through partial nitrification, a high nitrite accumulation rate (93.0 ± 3.8 %) was achieved by low dissolved oxygen (0.5–1.6 mg/L) and controlled aerobic time (3.5 h) in SBR. The UASB system was responsible for 78.8 ± 2.1 % nitrogen removal of the entire system with a hydraulic reaction time (HRT) of 3.8 h, accompanied by the anammox contribution up to 89.4 ± 6.0 %. The overall partial nitrification-simultaneous anammox and partial denitrification (PN-SAPD) system was controlled at a total COD/TIN of 2.8 ± 0.3 and a total HRT of only 10.2 h, achieving the nitrogen removal efficiency and effluent TIN were 95.2 ± 2.2 % and 3.4 ± 1.5 mg/L, respectively. The qPCR results showed functional genes (hzsA(B), hdh) associated with anaerobic ammonia-oxidizing bacteria (AnAOB), whose high gene copy abundance and transcription expression ensured the removal of major nitrogen from municipal wastewater and mature landfill leachate. 16S amplicon sequencing showed that the Ca. Brocadia (9.72–12.6 %) was further enrichment after sodium acetate was added, and the transcription expression of Thauera (0.5–7.0 %) caused nitrate to nitrite. The high abundance of related enzymes (hao, hzs, hdh, narGHI) involved in anammox and partial denitrification processes were found in the macrogenomic sequencing, and only Ca. Brocadia was involved in multi-pathway nitrogen metabolism in AnAOB. Based on the efficient nitrogen removal by AnAOB and denitrifying bacteria, this modified PN-SAPD process provides a new option for the co-treatment of mature landfill leachate in municipal wastewater treatment plants.

Enhanced granulation of activated sludge in an airlift reactor for organic carbon removal and ammonia retention from industrial fermentation wastewater: A comparative study

Ammonia retention and recovery from high-nitrogenous wastewater are new concepts being used for nitrogen management. A microaerophilic activated sludge system was developed to convert organic nitrogen into ammonia and retain it for its recovery; however, the settleability of activated sludge remains a challenge. Therefore, this study proposed an aerobic granular sludge system as a potential solution. Two types of sequencing batch reactors—airlift and upflow reactors—were operated to investigate the feasibility of fast granule formation, the performance of organic carbon removal and ammonia retention, and the dynamics of microbial community composition. The operation fed with industrial fermentation wastewater demonstrated that the airlift reactor ensured a more rapid granule formation than the upflow reactor because of the high shear force, and it maintained a superior ammonia retention stability of approximately 85 %. Throughout the operational period, changes in hydraulic retention time (HRT), settling time, and exchange ratio altered the granular particle sizes and microbial community compositions. Rhodocyclaceae were replaced with Comamonadaceae, Methylophilaceae, Xanthomonadaceae, and Chitinophagaceae as core taxa instrumental in granulation, likely because of their extracellular polymeric substance secretion. As the granulation process progressed, a significant decrease in the relative abundances of nitrifying bacteria—Nitrospiraceae and Nitrosomonadaceae—was observed. The reduction of settling time and HRT enhanced granulation and inhibited the activity of nitrifying bacteria. The success in granulation for ammonia conversion and retention in this study accelerates the paradigm shift from ammonia removal to ammonia recovery from industrial fermentation wastewater.

Effect of thermal shock and sustained heat treatment on mainstream partial nitrification and microbial community in sequencing batch reactors.

In order to develop a promising means of achieving mainstream short-cut nitrification, this study evaluated the effect of thermal shock on nitrite accumulation using intermittent offline and continuous inline heat treatment of biomass in sequencing batch reactors (SBRs). The SBRs fed with municipal wastewater were operated at a solid retention time of 7 days and nitrogen loading rate of 0.04 gN/L·d to 0.08 gN/L·d without the application of pre-treatment. Contrary to literature studies that showed suppression of nitrite-oxidizing bacteria at temperature 60 to 80 °C, nitrite accumulation was achieved temporarily when 20% of the biomass was heated for 2 h at 47 °C, as well as in continuously heated SBRs at 37 °C and 42 °C. The continuously heated reactors at 37 °C and 42 °C produced a maximum nitrite accumulation ratio (NAR) of 0.59 and 0.79, respectively, whereas the intermittent offline heating at 47 °C-2 h produced a NAR of 0.37. Although nitrite accumulation was stable only for 10-12 days in all heated reactors, this study demonstrates the achievement of mainstream partial nitrification (PN) at lower temperature (42 °C) than that reported in literature and also highlights the potential for achieving PN by implementing heat treatment of a portion of the return activated sludge (RAS) in biological nitrogen removal (BNR) systems. During the time when full nitrification was achieved, Nitrospira was more dominant than Nitrosomonas in all reactors at ratios of 1.4:1, 2.4:1, 2.4:1, and 3.7:1 for the control SBR (22 °C), 47 °C -2 h offline heating SBR, 37 °C SBR, and 42 °C SBR, respectively, suggesting that it may have played a role as a comammox bacteria capable of degrading ammonia to nitrates at elevated temperature.

Efficiency of wastewater treatment from environmental laboratory of science and technology, RMUTP using ozonation

Wastewater from the laboratory in the Division of Environmental Science and Technology, Faculty of Science and Technology, Rajamangala University of Technology Phra Nakhon is a source of pollution without any treatment. Researchers realized that it would become an environmental problem in the future. For this reason, wastewater treatment from water laboratory would be studied using ozonation process because the researchers want to use the existing equipment in the laboratory and a simple technique that the students can do. This aims to treat the wastewater until it passes the water standard. The laboratory's wastewater characteristics were unclear based on the study. The pH values measured were 8.15, 3.35, and 2.49, respectively. COD levels were monitored of 10,480, 2,400, and 3,040 mg/L, while the measured BOD values were 4,649, 1,683, and 2,200 mg/L, respectively. Additionally, the SS concentrations were 682, 788, and 548 mg/L, respectively. Because of these characteristics using only the biodegradation process didn’t work. The ozonation techniques were a good option following the research objective. The results showed that the suitable condition for the ozonation process was pH6 and the optimal time was 40 minutes. Because after 40 minutes the COD removal increase only 5% but after 60 minutes the ozonation had no effect on COD removal. This condition was applied before using biological treatment via a sequence batch reactor (SBR). Using microorganisms and 8 hours of aeration via SBR, the efficiency of COD BOD and SS removal was only 41.66, 0 and 71.82 percent, respectively. Filling ozone in rate 0.25 gram/hour/liter wastewater with SBR reactor, the wastewater treatment efficiency increased. It can remove COD, BOD and SS of 63.15, 50.00 and 72.63 percent, respectively. The advantage of this research was that the ozone generator can be applied for wastewater treatment without additional costs.

Innovative determination of the specific anammox activity for anammox sludge from continuous flow reactors: A comparison between continuous flow test and batch test

A novel method for measuring specific anammox activity (SAA) was proposed based on continuous flow tests to accurately determine the SAA of anammox sludge from continuous flow reactors, resolving the challenges of inaccurate SAA assessment caused by substrate shock to anammox bacteria. Results showed SAA of expanded granular sludge bed sludge via batch tests (0.101 ± 0.018 g-N·g-VSS-1·d-1) was lower than continuous flow tests (0.206 ± 0.010 g-N·g-VSS-1·d-1) (p < 0.05), highlighting the impact of substrate shock. Conversely, SAA of sequencing batch reactor sludge assessed via batch tests (0.878 ± 0.008 g-N·g-VSS-1·d-1) was higher than continuous flow tests (0.809 ± 0.005 g-N·g-VSS-1·d-1) (p < 0.01), attributed to endogenous denitrification. The advantages of continuous flow tests over batch tests included milder feeding way, stricter anaerobic conditions, and minimal sampling impact on system. Our study contributes to more accurate measurements of SAA of anammox sludge from continuous flow reactors, favoring long-term robust operation of anammox reactors.

Combined recovery of polyhydroxyalkanoates and reclaimed water in the mainstream of a WWTP for agro-food industrial wastewater valorisation by membrane bioreactor technology

The present study investigated the combined production of reclaimed water for reuse purposes and polyhydroxyalkanoates (PHA) from an agro-food industrial wastewater. A pilot plant implementing a two-stage process for PHA production was studied. It consisted of a mainstream sequencing batch membrane bioreactor (SBMBR) in which selection of PHA-accumulating organisms and wastewater treatment were carried out in, and a side-stream fed-batch reactor (FBR) where the excess sludge from the SBMBR was used for PHA accumulation. The performance of the SBMBR was compared with that of a conventional sequencing batch reactor (SBR) treating the same wastewater under different food to microorganisms’ ratios (F/M) ranging between 0.125 and 0.650 kgCOD kgTSS−3 d−1. The SBMBR enabled to obtain very high-quality effluent in compliance with the relevant national (Italy) and European regulations (Italian DM 185/03 and EU, 2020/741) in the field of wastewater reclamation, whereas the performances in the SBR collapsed at F/M higher than 0.50 kgCOD kgTSS−1d−1.A maximum intracellular storage of 45% (w/w) and a production yield of 0.63 gPHA L−1h−1 were achieved when the SBMBR system was operated with a F/M ratio close to 0.50 kgCOD kgTSS−1d−1. This resulted approximately 35% higher than those observed in the SBR, since the ultrafiltration membrane avoided the washout of dispersed and filamentous bacteria capable of storing PHA.Furthermore, while maximizing PHA productivity in conventional SBR systems led to process dysfunctions, in the SBMBR system it helped mitigate these issues by reducing membrane fouling behaviour. The results of this study supported the possibility to achieve combined recovery of reclaimed water and high-value added bioproducts using membrane technology, leading the way for agro-food industrial wastewater valorization in the frame of a circular economy model.

Application of a hybrid-fruit-peel (HFP) coagulant in low carbon source wastewater treatment as an external carbon source.

The application of a hybrid-fruit-peel (HFP) coagulant used as an external carbon source (ECS) in both simulated water and real sewage having a low carbon source treated with sequencing batch reactor (SBR) was studied, compared with that of sodium acetate (NaAc). The impact of HFP on sludge properties (such as extracellular polymer substance (EPS), dehydrogenase activity (DHA), charged property, size, microscopic images and bacteria phase) was characterized. The results showed that as an ECS, HFP basically gave similar nitrogen removal to NaAc and also gave a similar developing trend of both dissolved oxygen (DO) and pH. HFP promoted more proliferation of microorganisms and posed higher levels of protein (PN) and polysaccharide (PS) than NaAc, but gave slightly lower DHA than NaAc. After HFP was added as an ECS, the types and quantities of microorganisms increased significantly, the effluent qualities were improved and the sludge size and extensibility became larger, which was conducive to direct contact and remove pollutants. HFP played a similar role to NaAc as ECS and can be used as a quality and slow-releasing ECS for low carbon source wastewaters.

Copper oxide nanoparticles influence on the performance of sequencing batch reactor and the transfer of antibiotic resistance genes by regulating bacterial quorum sensing system

The environmental pollution caused by copper oxide nanoparticles (CuO NPs) has attracted widespread attention. In this study, microbial community, key metabolic pathways and quorum sensing (QS) were investigated to reveal the impact of copper oxide nanoparticles (CuO NPs) on the performance of sequencing batch reactor (SBR) and the transfer of antibiotic resistance genes (ARGs). Results showed that under CuO NPs impact, the removal efficiency of COD, ammonium nitrogen (NH4+-N) and total nitrogen (TN) was stable at around 90%, 96% and 60%, respectively. However, removal efficiency of total phosphorus (TP) was reduced to below 60% under 0.1 mg/L and 1 mg/L CuO NPs exposure. Microbial community analysis showed CuO NPs was advantageous to the growth of key functional bacterial phyla Bacteroidetes, Proteobacteria and Patescibacteria. The relative abundance of Candidatus Accumulibacter genus and ppX gene were decreased, which then lower the TP removal efficiency. Besides, CuO NPs might trigger QS system, affecting Candidatus Accumulibacter, Aeromonas genus and ppX gene, and then regulated the removal of TP in the SBR system. The co-occurrence network analysis revealed a correlation among core ARGs, signaling molecule, QS-related microbial community and ARGs’ hosts, suggesting that the spread of ARGs might be driven by QS system under selective pressure of CuO NP. These findings could reveal the effect of CuO NPs on the performance of activated sludge system and provides new ideas for ARGs control through regulating the QS system.

Mesophilic, thermophilic and thermal hydrolysis process coupled anaerobic digestion of sewage sludge: Biomethane potential, pathogen removal and energy feasibility

Conventional Anaerobic digestion is one of the most preferred sludge management techniques however, it has limitations such as low hydrolysis rate, longer retention time, larger digester volume, and reduced methane generation etc., To overcome these problems, Thermal hydrolysis process (THP) has been used as an effective pretreatment method for enhancing methane generation. However, the effect of the thermal hydrolysis process in heavy metal solubilization and methane production from high SRT (solids retention time) sludge is still not clear. Anaerobic digestion of sewage sludge sourced from sequencing batch reactor (SBR) and conventional activated sludge process (CASP) systems were evaluated under mesophilic (MAD), thermophilic (TAD), and thermal hydrolysis process (THP) mediated anaerobic digestion (AD) in terms of methane generation and, VS and pathogen removal. THP resulted in almost 38% COD solubilization for SBR and CASP sludge. Anaerobic digestion of SBR sludge resulted in methane generation of 101, 166, and 390 mL/gVS under mesophilic, thermophilic, and THP pretreatment conditions, respectively. For CASP sludge, the highest methane generation was observed for THP pretreated sludge (587 mL/gVS), followed by thermophilic (298 mL/gVS) and mesophilic digestion (241 mL/gVS). The fecal coliforms in the mesophilic and thermophilic digested SBR sludge were 3000 and 620 MPN/g, respectively, while 6100 and 740 MPN/g were found in digested CASP sludge, respectively. The THP-MAD and TAD processed SBR, and CASP sludges met the Class A sludge fecal coliforms and Salmonella density criteria of <1000 MPN/g TS (dry basis) and 3 MPN/4g TS (dry basis), respectively, over MAD processed sludges. Even though THP pretreatment resulted in the release of heavy metals, the concentration of heavy metals in the THP effluent was lesser than the limits prescribed by USEPA. The energy demand of the THP unit (160 °C, 6 bar) was estimated to be 162 MJ/m3. The energy balance analysis revealed that THP pretreatment would lead to energy self-sufficiency upon integration in a wastewater treatment plant (WWTP). This study can provide an experimental basis to apply THP pretreatment to enhance hydrolysis rate in anaerobic digesters while maximizing methane production and achieving energy benefits over MAD and TAD.

C4-HSL-mediated quorum sensing regulates nitrogen removal in activated sludge process at Low temperatures

The activated sludge process faces challenges in achieving adequate nitrification ability under low-temperature conditions. Therefore, we investigated the effects of different concentrations of exogenous N-butyryl-homoserine lactone (C4-HSL) on nitrogen removal in lab-scale sequencing batch reactors (SBRs) at 10 °C. The results revealed that both 10 and 100 μg/L of C4-HSL could improve NH4+-N removal efficiency by 26% and reduce the effluent TN concentration to below 15 mg/L. Analysis of extracellular polymeric substances (EPS) revealed that adding C4-HSL (especially 100 μg/L) reduced the protein-like substance content while increasing the humic and fulvic acid-like substance content in EPS. Protein-like substances could serve as carbon sources for denitrifiers, thus promoting denitrification. Moreover, exogenous C4-HSL increased the abundance of bacteria and genes associated with nitrification and denitrification. Further analysis of quorum sensing (QS) of microorganisms indicated that exogenous C4-HSL (especially 100 μg/L) promoted regulation, transportation, and decomposition functions in the QS process. Furthermore, CS, sdh, fum, and mdh gene expressions involved in the tricarboxylic acid (TCA) cycle were enhanced by 100 μg/L C4-HSL. Exogenous C4-HSL promoted microbial communication, microbial energy metabolism, and nitrogen metabolism, thereby improving the nitrogen removal efficiency of activated sludge systems at low temperatures. This study provides a feasible strategy for enhancing denitrogenation performance at low temperatures through exogenous C4-HSL.

Long-term presence of microplastics in aerobic and anaerobic sequential batch reactors: Effect on treatment, microbial diversity, and microplastics morphology

Sewage treatment plants (STPs) are identified as the significant sink and source of microplastics (MPs) in aquatic bodies and terrestrial systems. A major fraction of MPs gets retained in STPs for a longer duration, and their potentiality for secondary MPs generation and additives leaching remain under investigated. Therefore, this study focussed on the effect of long-term exposure of aerobic and anaerobic biological sewage treatment units on MPs, along with the effect of MPs on treatment efficiency and microbial consortium. A mixture of polyvinyl chloride, polystyrene, and nylon MPs at 262 MPs/L was spiked in the aerobic and anaerobic sequential batch reactors (SBRs) for 120 days at the start of study. The study revealed a release of noteworthy fraction of secondary MPs into the reactors from spiked MPs. At the end of 120th day, the presence of secondary generated MPs was estimated as 1000 ± 71 MPs/L and 650±141 MPs/L in aerobic and anaerobic SBRs respectively. Most of the observed secondary MPs were of size < 300 µm. Leaching of additives, i.e. cyclohexylamine, cyclotetradecane, octadecanol, pipericine etc., into the SBR effluents were also observed. The depuration capacities of the reactors were not affected with the presence of MPs during the study. While the microbial diversity and abundance were negatively impacted in aerobic SBRs, no such impacts were observed in anaerobic SBRs due to MPs. These results do suggest such exposures to potentially cause secondary MPs and chemical pollution in receiving matrices for the treated effluent, as well as effect on the native microbial community.

Pre-anaerobic phase persists benefits for nitrogen removal in high carbon/nitrogen scenarios: Comparative study of aerobic–anoxic and anaerobic–aerobic–anoxic sequencing batch reactors

The pre-anaerobic phase in anaerobic–aerobic–anoxic (AOA) process is critical to intensifying nitrogen removal at low C/N ratios, yet its applicability to high C/N scenarios remains vague. Here, four bench-scale aerobic–anoxic sequencing batch reactors (OA-SBRs) were established at different C/N ratios (3–16.5) and compared with the parallel AOA-SBRs. The results showed that the NH4+–N and TN removal in the OA-SBRs increased by 24.7% and 112.2%, respectively, as the C/N ratio elevated from 3 to 16.5, but they remained lower than those in the AOA-SBRs till carbon leakage. Typical cycle tests indicated that the pre-anaerobic phase probably endowed the endogenous nitrification/denitrification in the AOA-SBRs with kinetic advantages over the canonical ones in the OA-SBRs, supported by the improved enzymatic activities. Further, microbial insights identified more intensive niche competition between heterotrophic nitrifiers/denitrifiers and non-functional heterotrophs in the OA-SBRs, which could explain the poorer nitrogen removal without the pre-anaerobic phase. This study underscores the implications of pre-anaerobic phase in nitrogen removal of AOA process in high C/N scenarios.

New insight into the granule formation in the reactor for enhanced biological phosphorus removal.

While granulated activated sludge exhibits high productivity, the processes of granule formation are incompletely studied. The processes of granule formation and succession of communities were investigated in a laboratory sequencing batch reactor (SBR) under conditions for enhanced biological phosphorus removal (EBPR) using microbiological and molecular techniques. Active consumption of acetate, primarily by the phosphate-accumulating organisms (PAO), commenced at day 150 of cultivation. This was indicated by the high ratio of molar P-released/acetate uptake (0.73-0.77 P-mol/C-mol), characteristic of PAO. During this period, two types of granule-like aggregates formed spontaneously out of the activated sludge flocs. The aggregates differed in morphology and microbial taxonomic composition. While both aggregate types contained phosphorus-enriched bacterial cells, PAO prevailed in those of morphotype I, and glycogen-accumulating organisms (GAOs) were predominant in the aggregates of morphotype II. After 250 days, the elimination of the morphotype II aggregates from the reactor was observed. The subsequent selection of the community was associated with the development of the morphotype I aggregates, in which the relative abundance of PAO increased significantly, resulting in higher efficiency of phosphorus removal. Metagenomic analysis revealed a predominance of the organisms closely related to Candidatus Accumulibacter IС and IIС and of Ca. Accumulibacter IIB among the PAO. Based on the content of the genes of the key metabolic pathways, the genomes of potential PAO belonging to the genera Amaricoccus, Azonexus, Thauera, Zoogloea, Pinisolibacter, and Siculibacillus were selected. The patterns of physicochemical processes and the microbiome structure associated with granule formation and succession of the microbial communities were revealed.

Metagenomic analysis revealed the evolution of microbial communities, metabolic pathways, and functional genes in the heterotrophic nitrification-aerobic denitrification process under La3+ stress

Trivalent lanthanum (La3+) exists widely in ammonia nitrogen (NH4+-N) tailing water from ionic rare earth mines; however, its effect on heterotrophic nitrification-aerobic denitrification (HN-AD) is unknown, thereby limiting the application of the HN-AD process in this field. In this study, we conducted an HN-AD process using a sequencing batch reactor (5 L) that was continuously operated to directly treat acidic (NH4)2SO4 wastewater (influent NH4+-N concentration of approximately 110 mg/L and influent pH of 5) containing different La3+ concentrations (0–100 mg/L). The NH4+-N removal efficiency of the reactor reached 98.25 % at a La3+ concentration of 100 mg/L. The reactor was in a neutral-to-alkaline environment, which favored La3+ precipitation and complexation. Metagenomic analysis revealed that the relative abundance of Thauera in the reactor remained high (88.62–92.27 %) under La3+ stress. The relative abundances of Pannonobacter and Hyphomonas significantly increased, whereas that of Azoarcus significantly decreased. Metabolic functions in the reactor were mainly contributed by Thauera, and the abundance of metabolic functions under low La3+ stress (≤5 mg/L) significantly differed from that under high La3+ stress (≥10 mg/L). The relative abundance of ammonia assimilation-related genes in the reactor was high and significantly correlated with ammonia removal. However, traditional ammonia oxidation genes were not annotated, and unknown ammonia oxidation pathways may have been present in the reactor. Moreover, La3+ stimulated amino acid biosynthesis and translocation, the citrate cycle, sulfur metabolism, and oxidative phosphorylation and promoted the overproduction of extracellular polymeric substances, which underwent complexation and adsorbed La3+ to reduce its toxicity. Our results showed that the HN-AD process had a strong tolerance to La3+, stable NH4+-N removal efficiency, the potential to recover La3+, and considerable application prospects in treating NH4+-N tailing water from ionic rare earth mines.

Effect of different feeding strategies on performance of aerobic granular sludge: From perspective of extracellular polymeric substances and microorganisms

Long-term structural stability of aerobic granular sludge (AGS) is a major challenge in AGS biotechnology. This work investigated variations in the sludge performance, structural stability, and intrinsic mechanisms of AGS under three feeding strategies (R1 direct aeration after fast feeding, R2 anaerobic stirring after fast feeding and R3 anaerobic plug-flow slow feeding). The AGS sequencing batch reactor used in this study was in stable operation with biomass of 6–10 gMLSS/L and a hydraulic retention time of 4 h. The results showed that the best sludge performance was demonstrated in R3, followed by R2 and R1. These differences were caused by extracellular polymeric substances (EPS) and microbial communities. The excellent sludge performance in R3 was attributed to the high hydrophobicity in the EPS as well as the gel properties due to the high hydrogen bonding content in the exopolysaccharide (PS). Similarly, the gel properties formed by the high hydrogen bonding content maintain the sludge stability in R1. Slow-growing dominant bacteria were enriched in the AGS of R2 and R3 with alternating anaerobic/aerobic operation modes, resulting in outstanding sludge performance. In contrast, high abundance of the filamentous bacteria Neomegalonema was enriched in the AGS of R1 without an anaerobic phase, resulting in relatively poor sludge stability. Variations in EPS characteristics and microbial community under the different feeding strategies were the main mechanisms responsible for the long-term operation of AGS. This study could provide a theoretical foundation and technical assistance for the sustainable operation of AGS reactors.

Treatment of sludge from intensive whiteleg shrimp farming using a sequencing batch reactor

The wastewater/sludge generated from the shrimp aquaculture industry contains high levels of nitrogen (N), phosphorous (P), and carbon (C). This study aimed to evaluate the efficacy of N, P, and C removal and recovery from sludge obtained during the siphoning process of intensive white leg shrimp farming by using a sequencing batch reactor (SBR) with two trials. In the first trial, reactors were operated aerobically (3 - 5 days) and anaerobically (4 - 6 days) in sequence, resulting in a total cycle time of 9 days. In trial 2, the reactors were run aerobically for the first 3, 4, &amp; 5 days, respectively, succeeded by anoxic conditions until the end of the experiment on day 14. The results showed that the removal of total ammonia nitrogen and chemical oxygen demand was about 60 - 70%, but the treatment efficiencies of total N and P were extremely low. Moreover, the anaerobic mode improved the mineralization of P, while aerobic condition promoted nitrate production. Further studies are needed to improve the nutrient and organic removal performance of the SBR.

Chloroxylenol positively affects the aerobic sequencing batch reactor performance and reshapes microbial communities and antibiotic resistance genes

The antibacterial ingredient chloroxylenol (PCMX) has been frequently detected in various environments, raising increasing concerns about its potential risks to organisms and ecosystems. Herein, the impacts of PCMX on the performance and microbial communities of aerobic sequencing batch reactors were investigated. The results demonstrated that 0.1 and 1.0 mg/L PCMX did not affect the removal performance of COD and NH4+-N, as well as the production of extracellular polymeric substances. However, a notable enhancement in these performances was observed when the concentration of PCMX reached 10 mg/L. Analysis of microbial community revealed that exposure to PCMX did not alter α diversity but significantly shifted the community structure. Spirosoma and Ferruginibacter were identified as dominant genera across all stages in the sludge, with Ferruginibacter being inhibited by 10 mg/L PCMX (from 20.3 % to 11.1 %). Network analysis revealed that PCMX had a diminishing effect on the interaction and complexity of microbial. High throughput qPCR analysis indicated that PCMX reshaped the antibiotic resistance genes (ARGs). The relative gene copy number increased from 0.23 to 0.29 at a concentration of 0.1 mg/L PCMX, while it decreased from 0.51 to 0.40 by 10 mg/L PCMX. Genes tetX and sul2 were identified as dominant genes unaffected by 0.1 and 1.0 mg/L but significantly inhibited by 10 mg/L PCMX. These findings suggested that environmental levels of PCMX might promote the enrichment of ARGs, while higher concentrations impeded gene propagation. This study provides new information regarding the roles, potential risks, and removal strategies of PCMX in wastewater treatment processes.

Effects of combined polystyrene nanoplastics and ibuprofen on the activated sludge processes: Short-term exposure in pure cultures versus long-term stress in mixed communities

Given their large production and widespread application, polystyrene nanoplastic (Nano-PS) and ibuprofen (IBU), as particularly relevant compounds, are found to coexist in wastewater treatment plants. In this study, the effect of combined Nano-PS and IBU on the activated sludge processes was investigated through short-term exposure in pure cultures and long-term stress in mixed communities. This study found that the inhibition of SO42− removal by sulfate-reducing bacteria was more pronounced for small-sized Nano-plastics in motion state. Compared to individual pollutants, the toxicity of Nano-PS + IBU mixture showed antagonistic effects at low dosage and synergistic effect at high dosage. In mixed communities, COD and TN removal of sequencing batch reactor decreased from 93.3 % and 90.7 % to 80.1 % and 64.5 % with increasing Nano-PS/IBU. The membrane integrity was destroyed by excess reactive oxygen species, further reducing the protein of extracellular polymeric substances. Furthermore, Nano-PS/IBU altered the community structure, decreased microbial diversity and affected the distribution of abundance and rare taxa. These adverse effects, accompanied by a decline in the relative abundance of nitrifiers (e.g., Nitrosomonas and Ellin6067) and genes (e.g., AmoCAB and Hao), directly accounted for the strong deterioration observed in ammonia oxidation. This study provided important information on the effects of nanoplastic and pharmaceutical compounds on activated sludge system, which is a hidden danger to nitrogen removal in biological treatment and should give more attention.