Motivated by the basic biological concern of attributing phenotypic variation to genotypic versus environmental causes, the general principles of assigning effects to two causal variables and specifying the operation by which they produce (phenotypic) responses are elaborated. It turns out that the effects of a cause can be defined only if the response to it is consistently distinguishable under the operation of the complementary causes. If consistency is realized for all causes of one variable, the effects of this causal variable are termed separable. Interaction occurs when separability is not realized and thus effects are not definable for at least some causes. As a consequence, separability or interaction may be realized for one but not for the other causal variable, or it may be realized for both variables simultaneously. The concept of unidirectional interaction or separability provides new means of interpretation of data. Additivity of effects, even in its most general sense, is shown to be a particular case of mutual separability (separability for both variables). It is shown that there are situations which can be transformed into additivity, thus implying mutual separability, which would definitely have been classified as interaction according to the classical concepts. Additive responses actually form only a vanishingly small proportion among the mutually separable ones. This is demonstrated with the help of necessary conditions for additivity. Therefore, the additive basis of the analysis of variance is not suitable for detecting interaction. The probabilistic interpretation of the concept of separability is outlined, and the problem of using averages for defining effects is discussed and demonstrated.
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