Sensu Research Articles

Incidence of symptomatic Lyme borreliosis in nine European countries

ObjectivesTo better understand the Lyme borreliosis (LB) burden in Europe, we aimed to estimate the incidence of symptomatic Borrelia burgdorferi sensu lato (Bbsl) infections after adjusting public health LB surveillance data for under-detection of symptomatic Bbsl infections. MethodsData from seroprevalence studies and estimates of the symptomatic proportion and duration of antibody detection in Bbsl-infected individuals, derived from reviews of the published literature, were used to adjust public health LB surveillance data to estimate the incidence of symptomatic Bbsl infection in nine European countries from 2018 to 2022. ResultsThe prevalence of anti-Bbsl antibodies ranged from 2.3% in Romania to 9.4% in Germany. Under-detection multipliers varied across surveillance systems; using 10-year duration of antibody detection, multipliers were 2.4-10.5 in countries reporting all LB cases and 54.6-722.2 in countries reporting only Lyme neuroborreliosis cases. The incidence of symptomatic Bbsl infection adjusted for under-detection was highest in Finland, Germany, Norway, Poland, and Switzerland, intermediate in the Czech Republic and Denmark, and lowest in Ireland and Romania. ConclusionAdjustment of LB surveillance for under-detection found a high incidence of symptomatic Bbsl infection in several European countries. Differences in LB surveillance systems should be considered when comparing surveillance data between countries and when estimating LB disease burden.

Antimicrobial and Phylogenomic Characterization of Bacillus cereus Group Strains Isolated from Different Food Sources in Italy.

Background:Bacillus cereus is a widespread environmental Gram-positive bacterium which is especially common in soil and dust. It produces two types of toxins that cause vomiting and diarrhea. At present, foodborne outbreaks due to Bacillus cereus group bacteria (especially Bacillus cereus sensu stricto) are rising, representing a serious problem in the agri-food supply chain. Methods: In this work, we analyzed 118 strains belonging to the Bacillus cereus group, isolated from several food sources, for which in vitro and in silico antibiotic resistance assessments were performed. Results: Many strains showed intermediate susceptibility to clindamycin, erythromycin, and tetracycline, suggesting an evolving acquisition of resistance against these antibiotics. Moreover, one strain showed intermediate resistance to meropenem, an antibiotic currently used to treat infections caused by Bacillus cereus. In addition to the phenotypic antimicrobial resistance profile, all strains were screened for the presence/absence of antimicrobial genes via whole-genome sequencing. There was inconsistency between the in vitro and in silico analyses, such as in the case of vancomycin, for which different isolates harbored resistance genes but, phenotypically, the same strains were sensitive. Conclusions: This would suggest that antibiotic resistance is a complex phenomenon due to a variety of genetic, epigenetic, and biochemical mechanisms.

On Seedling Morphoanatomy of Asteraceae Campestral Species: Some Adaptative and Taxonomic Considerations

Theoretical Framework: The vegetation of Parana State fields is composed mainly of woody grassy savanna, and one of the largest families in specific richness is Asteraceae. The campestral Asteraceae occurs in three microhabitats as dry fields (steppes “stricto sensu”), rocky outcrops (rupestrian vegetation refuges) and humid fields (higrophyllous steppes). Morphology and structure of Asteraceae seedlings can be useful for species recognition in the field at the juvenile stage, and to understand how seedlings establish themselves in different microhabitats. Objective: The aim of this paper was to analyze the seedling structure of 17 Asteraceae species, and to summarize some considerations on anatomical characters of the seedlings, which may be variations environmentally induced and they have some taxonomic or evolutionary value. Method: Cipselae of 17 species of Asteraceae were collected in the state parks of Paraná, Brazil, and placed to germinate in Petri plates. The seedling development occurred in greenhouse. Root, hypocotyl, cotyledons, epicotyl and eophylls were sectioned in rotation microtome, and analyzed in light microscope. Cotyledons and eophylls were investigated in a scanning electronic microscope. Results: Roots are diarch or tetrarch. Hypocotyl may have the root structure and partially or wholly as transition root-stem structure. Epicotyl shows the stem structure. Cotyledons and eophylls are dorsiventral. Trichome density and amphistomaty are xeromorphic characters that occur in the cotyledons and eophylls of the species. Conclusion: Some structural characters have diagnostic value for separation of investigated species, such as hypocotyl structure, root/stem transition type, epidermis, mesophyll tissues and midrib saliency of the cotyledons and eophylls, and presence of secretory ducts.

Comparison of three LAMP protocols for the simultaneous detection of DNA from species that produce cystic echinococcosis

Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by species of the Echinococcus granulosus sensu lato complex. Different types of canids may act as definitive hosts by eating raw viscera infected with fertile hydatid cysts. The intermediate host (mainly ungulates) and humans acquire the infection through the fecal oral route (i.e. egg ingestion). Globally, more than 1 million people are affected by CE, causing a loss of 1–3 million disability-adjusted life years (DALYs) and a financial burden of US$ 3 billion annually. Loop mediated isothermal amplification (LAMP) protocols promise to be a useful tool to detect DNA, providing a low cost and thermocycle-free methodology. Given that surveillance for CE can be performed in feces from canids or other environmental matrixes contaminated with eggs, the characteristics of a LAMP protocol would favor implementation in endemic areas with basic resources. Herein, we compared three LAMP protocols for the simultaneous detection of E. granulosus s.l. species that cause CE. This comparation was carried with DNA obtained from different stages of E. granulosus s.l. Two of these are newly developed protocols that showed good analytical sensitivity and specificity. In both cases, the use of malachite green dye to directly visualize the test result was possible. From these two new LAMP protocols, one had better values for the detection of DNA from different types of E. granulosus s.l. DNA samples. Therefore, through this study, we provide a low-cost new tool for DNA detection of E. granulosus s.l. in poorly equipped laboratories from endemic areas.

Hand Carriage of Yeast in Student of Medicine, Nursing, and Medical Laboratory Science: Impact of Infection Control Measures.

We studied yeast hand carriage of 260 healthcare students. We isolated yeasts in 27 students (10.4%), without differences between medicine, nursing, and medical laboratory science programs and gender. A significant lower prevalence of carriage was shown in the clinical cycle (2.7%) compared to the basic cycle (13.5%) (p = 0.022) and the preclinical cycle (13.5%) (p = 0.014). Increased handwashing frequency and the use of alcohol gel and antiseptic soap decreased yeast carriage. Students who applied moisturizing hand cream two or more times a day had a lower frequency of yeast carriage (3.4%) than those who did not use it or used it once a day (16.5%), showing a significant difference (p = 0.016). The most prevalent species was C. parapsilosis sensu stricto (81.5%), followed by Meyerozyma guilliermondii (C. guillermondii) (7.4%), Trichosporon mucoides (7.4%), and R. mucilagenosa (3.7%). One case showed mixed carriage of C. parapsilosis and C. albicans. All strains were sensitive to voriconazole, caspofungin, and anidulafungin. This study shows hand carriage of yeast in health students, mainly by C. parapsilosis, and the frequency of infection control measures and moisturizing hand cream is associated with colonization control.

Wave attenuation by Cystoseira sensu lato in two shallow Mediterranean coastal waters

Coastal protection from erosion is an important ecosystem service provided by marine vegetation. Seaweeds and seagrasses can attenuate wave action in coastal areas and increase sediment retention. Here we investigated for the first time the effects of erect Cystoseira sensu lato forests in attenuating the wave energy in two Mediterranean shallow areas (Greece and Tunisia). The hydrodynamic effects were measured using the dissolution rate of gypsum cones (fd) r. Experiments were carried out in Greece, on the species Ericaria barbatula, and in Tunisia on the species E. brachycarpa and Cystoseira crinitophylla. Wave attenuation was directly related to percent cover of these macroalgal forests under different spatial and temporal conditions. On average, Cystoseira s.l. forests with 100 % cover reduced the wave action by 38 % more than the control (0 % cover). This study shows that Cystoseira s.l. forests under specific conditions play a key role for protecting the Mediterranean coasts from erosion, and can present a nature-based solution as an alternative to unsustainable and costly artificial constructions.

A novel strategy for enhancing high solid anaerobic digestion of fecal slag and food waste using percolate recirculation and dosage of nano zero-valent iron

To speed up reaching UN Sustainable Development Goal 6 for safe sanitation by 2030, integrating high-solid anaerobic digestion (HSAD) into decentralized systems could recycle fecal slag (FS) and food waste (FW), aiding a circular economy and toilet revolution. In this study, a percolate recirculation system and conductive material were used to improve mass transfer, stability, and enhance methane production in HSAD of FS and FW. This setup consists of a percolate tank and a digester tank, where nano-zero valent iron (nZVI) was dosed in the percolate tank (PnZVI in P) and the digester tank (PnZVI in D) and compared with a control with no additive (PControl). The highest cumulative methane yield of 519.43 mL/gVS was achieved in PnZVI in D, which was 4.52 and 3.59 times higher than that of PControl (144.59 mL/gVS) and PnZVI in P (114.96 mL/gVS). This finding demonstrates that the dosing strategy of PnZVI in D facilitated effective interaction among organic matter, microbial communities, and nZVI, resulting in organics removal efficiencies of 67.42 % (total solid) and 77.22 % (volatile solid). Moreover, microbial community analysis supported the efficacy of the PnZVI in D strategy, revealing the enrichment of Clostridium sensu stricto 1 (46.91 %), which potentially engaged in interspecies electron transport (Interspecies hydrogen transfer (IHT) and direct interspecies electron transfer (DIET)) with Methanobacterium (81.19 %) and Methanosarcina (17.11 %). These interactions contribute to enhanced methane yield and stability maintenance in the HSAD system with percolate recirculation. The findings of this study demonstrate that the implementation of HSAD of FS and FW, coupled with percolate recirculation and the addition of nZVI, holds promise for enabling sustainable sanitation practices in developing regions. Moreover, this approach not only facilitates resource recovery but also eliminates the requirement for water.

Diversity and impact of single-stranded RNA viruses in Czech Heterobasidion populations.

Heterobasidion annosum sensu lato comprises some of the most devastating pathogens of conifers. Exploring virocontrol as a potential strategy to mitigate economic losses caused by these fungi holds promise for the future. In this study, we conducted a comprehensive screening for viruses in 98 H. annosum s.l. specimens from different regions of Czechia aiming to identify viruses inducing hypovirulence. Initial examination for dsRNA presence was followed by RNA-seq analyses using pooled RNA libraries constructed from H. annosum and Heterobasidion parviporum, with diverse bioinformatic pipelines employed for virus discovery. Our study uncovered 25 distinct ssRNA viruses, including two ourmia-like viruses, one mitovirus, one fusarivirus, one tobamo-like virus, one cogu-like virus, one bisegmented narna-like virus and one segment of another narna-like virus, and 17 ambi-like viruses, for which hairpin and hammerhead ribozymes were detected. Coinfections of up to 10 viruses were observed in six Heterobasidion isolates, whereas another six harbored a single virus. Seventy-three percent of the isolates analyzed by RNA-seq were virus-free. These findings show that the virome of Heterobasidion populations in Czechia is highly diverse and differs from that in the boreal region. We further investigated the host effects of certain identified viruses through comparisons of the mycelial growth rate and proteomic analyses and found that certain tested viruses caused growth reductions of up to 22% and significant alterations in the host proteome profile. Their intraspecific transmission rates ranged from 0% to 33%. Further studies are needed to fully understand the biocontrol potential of these viruses in planta.IMPORTANCEHeterobasidion annosum sensu lato is a major pathogen causing significant damage to conifer forests, resulting in substantial economic losses. This study is significant as it explores the potential of using viruses (virocontrol) to combat these fungal pathogens. By identifying and characterizing a diverse array of viruses in H. annosum populations from Czechia, the research opens new avenues for biocontrol strategies. The discovery of 25 distinct ssRNA viruses, some of which reduce fungal growth and alter proteome profiles, suggests that these viruses could be harnessed to mitigate the impact of Heterobasidion. Understanding the interactions between these viruses and their fungal hosts is crucial for developing effective, environmentally friendly methods to protect conifer forests and maintain ecosystem health. This study lays the groundwork for future research on the application of mycoviruses in forest disease management.

Whole-genome sequencing of major malaria vectors reveals the evolution of new insecticide resistance variants in a longitudinal study in Burkina Faso

BackgroundIntensive deployment of insecticide based malaria vector control tools resulted in the rapid evolution of phenotypes resistant to these chemicals. Understanding this process at the genomic level is important for the deployment of successful vector control interventions. Therefore, longitudinal sampling followed by whole genome sequencing (WGS) is necessary to understand how these evolutionary processes evolve over time. This study investigated the change in genetic structure and the evolution of the insecticide resistance variants in natural populations of Anopheles gambiae over time and space from 2012 to 2017 in Burkina Faso.MethodsNew genomic data have been generated from An. gambiae mosquitoes collected from three villages in the western part of Burkina Faso between 2012 and 2017. The samples were whole-genome sequenced and the data used in the An. gambiae 1000 genomes (Ag1000G) project as part of the Vector Observatory. Genomic data were analysed using the analysis pipeline previously designed by the Ag1000G project.ResultsThe results showed similar and consistent nucleotide diversity and negative Tajima’s D between An. gambiae sensu stricto (s.s.) and Anopheles coluzzii. Principal component analysis (PCA) and the fixation index (FST) showed a clear genetic structure in the An. gambiae sensu lato (s.l.) species. Genome-wide FST and H12 scans identified genomic regions under divergent selection that may have implications in the adaptation to ecological changes. Novel voltage-gated sodium channel pyrethroid resistance target-site alleles (V402L, I1527T) were identified at increasing frequencies alongside the established alleles (Vgsc-L995F, Vgsc-L995S and N1570Y) within the An. gambiae s.l. populations. Organophosphate metabolic resistance markers were also identified, at increasing frequencies, within the An. gambiae s.s. populations from 2012 to 2017, including the SNP Ace1-G280S and its associated duplication. Variants simultaneously identified in the same vector populations raise concerns about the long-term efficacy of new generation bed nets and the recently organophosphate pirimiphos-methyl indoor residual spraying in Burkina Faso.ConclusionThese findings highlighted the benefit of genomic surveillance of malaria vectors for the detection of new insecticide resistance variants, the monitoring of the existing resistance variants, and also to get insights into the evolutionary processes driving insecticide resistance.

A sustainable integration of mining activity in a tourist mountain territory: The case of GermanascaValley

Every mining activity shows a footprint on the territory. The signs left by mining operations are physical, such as tunnels, extractive waste facilities, dressing plants, but also economic and social, due to job placement, income and knowledge connected to mining activity sensu lato. In the extraordinary context of the Germanasca Valley, mining has coexisted for hundreds of years with the mountain environment of the Alps and with the local population, and has become a fundamental part of local development, intimately connected with the territory and the local economic and social fabrics.The progressive migration of mining crops at lower altitudes has left behind old mining structures that here, more than elsewhere, guaranteed new forms of industrial and geo-tourism. Similarly, the technological advance and the evolution of mining production, towards a higher quality product, with a consequent reduction in the quantities exploited, led over time to a progressive reduction in the number of employed miners. The resilience of the local population, however, balanced the contraction of work in the mine, transforming former miners in tourist guides and increasing the induced activities, passing from the previous “in house” model to an “outsourcing” model, characterized by external management of mine-related activities. The transformation of old mines into museums has certainly contributed to the development of the area, particularly considering a slow tourism that shows scarce attitude to a “fast and junk” tourism. The challenge is to understand if and how geotourism influence, in specific mining areas (such as Germanasca Valley), the attractiveness of a place.

Phaeobacter italicus JN-W1: A pathogenic bacterium causing bleaching disease in Porphyra sensu lato (Bangiales, Rhodophyta)

The recent emergence of bleaching disease in the shell-borne conchocelis (SBC) of Porphyra sensu lato has escalated with alarming speed, inflicting severe economic losses on the Porphyra aquaculture industry. Despite the severity of the outbreak, the causative agents of this disease have remained elusive. In this study, we successfully isolated and purified the pathogenic bacterium JN-W1 from SBC affected by bleaching disease. Our infection assays revealed that the bacterium induced the degradation of algal cell organelles and the formation of autophagosomes, ultimately leading to the conversion of dead cells into empty-walled remnants, likely accounting for the bleaching symptoms observed in Porphyra. Additionally, the infection caused severe impairment of the algal photosynthetic apparatus, evidenced by a marked reduction in photosynthetic efficiency as the infection advanced. Through a combination of single-copy orthologous gene analysis and detailed morphological, physiological, and biochemical characterization, strain JN-W1 was definitively classified as Phaeobacter italicus JN-W1. The bacterium demonstrated optimal growth and infection capabilities at 25 °C, pH 7.0, and a salinity of 20. JN-W1's pathogenicity was found to be contact-dependent, mediated by intracellular substances. This study laid a crucial foundation for further exploration of JN-W1's virulence mechanism and the development of effective strategies to prevent and control the outbreaks of Porphyra bleaching disease.

The impact of Bacillus thuringiensis var. israelensis (Vectobac® WDG) larvicide sprayed with drones on the bio-control of malaria vectors in rice fields of sub-urban Kigali, Rwanda

BackgroundThe core vector control tools used to reduce malaria prevalence are currently long-lasting insecticidal nets (LLINs), and indoor residual spraying (IRS). These interventions are hindered by insecticide resistance and behavioural adaptation by malaria vectors. Thus, for effective interruption of malaria transmission, there is a need to develop novel vector control interventions and technologies to address the above challenges. Larviciding using drones was experimented as an innovative tool that could complement existing indoor interventions to control malaria.MethodsA non-randomized larviciding trial was carried out in irrigated rice fields in sub-urban Kigali, Rwanda. Potential mosquito larval habitats in study sites were mapped and subsequently sprayed using multirotor drones. Application of Bacillus thuringiensis var. israelensis (Bti) (Vectobac® WDG) was followed by entomological surveys that were performed every two weeks over a ten-month period. Sampling of mosquito larvae was done with dippers while adult mosquitoes were collected using CDC miniature light traps (CDC-LT) and pyrethrum spraying collection (PSC) methods. Malaria cases were routinely monitored through community health workers in villages surrounding the study sites.ResultsThe abundance of all-species mosquito larvae, Anopheles larvae and all-species pupae declined by 68.1%, 74.6% and 99.6%, respectively. Larval density was reduced by 93.3% for total larvae, 95.3% for the Anopheles larvae and 61.9% for pupae. The total adult mosquitoes and Anopheles gambiae sensu lato collected using CDC-Light trap declined by 60.6% and 80% respectively. Malaria incidence also declined significantly between intervention and control sites (U = 20, z = − 2.268, p = 0.023).ConclusionsThe larviciding using drone technology implemented in Rwanda demonstrated a substantial reduction in abundance and density of mosquito larvae and, concomitant decline in adult mosquito populations and malaria incidences in villages contingent to the treatment sites. The scaling up of larval source management (LSM) has to be integrated in malaria programmes in targeted areas of malaria transmission in order to enhance the gains in malaria control.

Phylogenomic and molecular marker based studies to clarify the evolutionary relationships amongst Anoxybacillus species and demarcation of the family Anoxybacillaceae and some of its constituent genera.

The family Anoxybacillaceae was recently proposed encompassing the genera Anoxybacillus, Geobacillus, Parageobacillus, Saccharococcus and Thermolongibacillus. Of these genera, Anoxybacillus contains >50% of the Anoxybacillaceae species. However, Anoxybacillus species form multiple unrelated clades in phylogenetic trees and their evolutionary relationships are unclear. To clarify the evolutionary relationships of Anoxybacillus and other Anoxybacillaceae species, detailed phylogenomic and comparative analyses were conducted on 38 Anoxybacillaceae species with available genomes. In a phylogenomic tree based on 1148 core proteins, all Anoxybacillus, Geobacillus, Parageobacillus, Saccharococcus and Thermolongibacillus species, excepting Anoxybacillus sediminis, formed a strongly supported clade representing the family Anoxybacillaceae. Five conserved signature indels (CSIs) reported here are also uniquely found in these species, providing robust means for the demarcation of family Anoxybacillaceae in molecular terms. In our phylogenomic tree and in the Genomic Taxonomy Database, Anoxybacillus species formed four distinct clades designated as Anoxybacillus sensu stricto (containing the type species A. pushchinoensis), Anoxybacillus_A, Anoxybacillus_B and Anoxybacillus_C. Our analyses have identified 17 novel CSIs which offer means to reliably distinguish species from these clades based upon multiple uniquely shared molecular characteristics. Additionally, we have identified three and seven CSIs specific for the genera Geobacillus and Brevibacillus, respectively. All seven Brevibacillus-specific CSIs are also shared by Anoxybacillus sediminis, which branches reliably with this genus. Based on the strong phylogenetic and molecular evidence presented here, we are proposing that the genus Anoxybacillus should be restricted to only the species from Anoxybacillus sensu stricto clade, whereas the species from Anoxybacillus_A, Anoxybacillus_B, and Anoxybacillus_C clades should be transferred into three novel genera Anoxybacteroides gen. nov., Paranoxybacillus gen. nov. and Thermaerobacillus gen. nov., respectively. Additionally, we are also proposing the transfer of Anoxybacillus sediminis to the genus Brevibacillus. The proposed changes, which reliably depict the evolutionary relationships among Anoxybacillaceae species, should be helpful in the studies of these organisms.

How life became colourful: colour vision, aposematism, sexual selection, flowers, and fruits.

Plants and animals are often adorned with potentially conspicuous colours (e.g. red, yellow, orange, blue, purple). These include the dazzling colours of fruits and flowers, the brilliant warning colours of frogs, snakes, and invertebrates, and the spectacular sexually selected colours of insects, fish, birds, and lizards. Such signals are often thought to utilize pre-existing sensitivities in the receiver's visual systems. This raises the question: what was the initial function of conspicuous colouration and colour vision? Here, we review the origins of colour vision, fruit, flowers, and aposematic and sexually selected colouration. We find that aposematic colouration is widely distributed across animals but relatively young, evolving only in the last ~150 million years (Myr). Sexually selected colouration in animals appears confined to arthropods and chordates, and is also relatively young (generally <100 Myr). Colourful flowers likely evolved ~200 million years ago (Mya), whereas colourful fruits/seeds likely evolved ~300 Mya. Colour vision (sensu lato) appears to be substantially older, and likely originated ~400-500 Mya in both arthropods and chordates. Thus, colour vision may have evolved long before extant lineages with fruit, flowers, aposematism, and sexual colour signals. We also find that there appears to have been an explosion of colour within the last ~100 Myr, including >200 origins of aposematic colouration across nine animal phyla and >100 origins of sexually selected colouration among arthropods and chordates.

Large-Scale Sampling Reveals the Strain-Level Diversity of Burkholderia Symbionts in Riptortus pedestris and R. linearis (Hemiptera: Alydidae).

Burkholderia (sensu lato) is a diverse group of β-Proteobacteria that exists worldwide in various environments. The SBE clade of this group was thought to be mutualistic with stinkbugs. Riptortus-Burkholderia was suggested as an ideal model system for studying insect-microbe symbiosis. To explore the strain-level diversity of Burkholderia at the individual and population levels of Riptortus stinkbugs (Hemiptera: Alydidae), and to uncover the factors affecting the Burkholderia community, large-scale sampling of two Riptortus species and deep sequencing data (16S amplicon) were used in the present study. Our results showed that: (1) the proportions of facultative symbiotic bacteria Burkholderia were very high, with an average proportion of 87.1% in the samples; (2) only six out of 1373 Burkholderia amplicon sequence variants (ASVs) did not belong to the SBE clade, accounting for only 0.03% of Burkholderia; (3) a relatively small number of Burkholderia ASVs had a large number of sequences, with 22, 54, and 107 ASVs accounting for more than 1.0%, 0.1%, and 0.01% of the total Burkholderia sequences, respectively; (4) multiple Burkholderia ASVs were present in most Riptortus individuals, but there was one dominant or two codominant ASVs, and codominance was more likely to occur when the genetic distance between the two codominant ASVs was small; and (5) the beta diversity of Burkholderia was significantly different between the two host species (PerMANOVA: both Jaccard and Bray-Curtis, p < 0.001) and among localities (PerMANOVA: both Jaccard and Bray-Curtis, p < 0.001). Two-way PerMANOVA also indicated that both the host (Bray-Curtis, p = 0.020; Jaccard, p = 0.001) and geographical location (Bray-Curtis, p = 0.041; Jaccard, p = 0.045) influence Burkholderia communities; furthermore, Mantel tests showed that the Burkholderia communities were significantly correlated with the geographical distance of sample locations (R = 0.056, p = 0.001). Together, our findings demonstrate the fine-scale diversity of Burkholderia symbionts and suggest a region- and host-dependent pattern of Burkholderia in Riptortus stinkbugs.

Dietary supplementation with a blend composed of carvacrol, tannic acid derived from Castanea sativa and Glycyrrhiza glabra, and glycerides of medium chain fatty acids for weanling piglets raised in commercial farm.

This study aimed to evaluate the dietary administration of a blend composed of carvacrol, tannic acid derived from Castanea sativa mill and Glycyrrhiza glabra, medium chain fatty acids (MCFAs) glycerides for weanling piglets. An in vitro digestion followed by total phenolic content (TPC) and total antioxidant activity (TAC) assessment was performed before the in vivo application. At weaning, a total of 210 piglets were randomly allocated to two experimental treatments (7 replicates/15 piglets for each replicate). Control group (CTR) was fed a standard basal diet while the treated group (T) was fed the basal diet mixed with 1.500mg/kg of blend. After in vitro digestion, TPC and TAC evidenced peaks at the end of oral and gastric phases in comparison to the intestinal one in line with the high content of phenolic compound (P < 0.05). Treatment conditioned body weight and average daily gain (P < 0.05), fecal score on 6, 7, and 8 d after weaning (P < 0.05). At 35d, the T group showed a decrease in salivary cortisol compared to CTR (P < 0.05). Duodenum and jejunum sections of T piglets revealed higher villi (P < 0.05), deeper crypts (P < 0.01), and increased V/C ratio (P < 0.01). CTR showed a higher expression of duodenal Occludin (P < 0.05). Jejunal E-cadherin and Occludin were more expressed in T jejunum sections (P < 0.05). Twelve differentially abundant genera were identified in T group caecal samples. Potentially harmful Clostridium sensu stricto 13 was reduced by the treatment (P < 0.05). In conclusion, the tested blend positively affected salivary stress markers and the gut health of weaned piglets.

233 Exploring the impact of intrauterine growth restriction on fecal microbiota and metabolome in pigs

Abstract Intrauterine growth restriction (IUGR) poses a significant challenge in swine production, affecting approximately 20% of piglets and resulting in increased mortality rates, metabolic disturbances, compromised intestinal development, and altered microbial colonization. This phenomenon arises as the IUGR fetus adapts to limited oxygen and nutrient supply in utero by reallocating blood flow towards vital organs, particularly the brain, consequently leading to a greater brain-to-liver weight ratio (BrW/LW) at birth. Our study aimed to comprehensively investigate the repercussions of IUGR on fecal microbiota composition and plasma metabolome profiles in pigs from birth through to slaughter. To elucidate these effects, we conducted computed tomography scans on piglets within 24 h of birth to assess brain and liver weights accurately. Subsequently, we selected two groups of piglets based on the BrW/LW. Specifically, 12 piglets with the greatest BrW/LW (IUGR; BrW/LW: 1.00 ± 0.09) and 12 with the least BrW/LW (NORM; BrW/LW: 0.36 ± 0.04). From these piglets we collected fecal and blood samples at specific time points: during lactation (T1: d 16 ± 0.6), after the starter period (T2: d 63 ± 8.6), and at the onset (T3: d 119 ± 11.4) and conclusion (T4: d 162 ± 14.3) of the finisher period. Throughout the study, all pigs received identical diets to eliminate dietary confounders. In the lactation, starter and grower but not finisher period, IUGR piglets grew 47, 19 and 14% slower, respectively compared with their NORM littermates. Our findings revealed that fecal microbial α-diversity remained unaffected by IUGR across all time points examined, indicating a stable microbial richness within the gut microbiota of IUGR and NORM pigs. However, β-diversity, reflecting inter-group microbial community variation, exhibited significant differences at T1 (P = 0.002), T2 (P = 0.079), and T3 (P = 0.027), suggesting distinct microbial compositions between IUGR and NORM pigs at these stages. Specifically, IUGR pigs displayed a greater abundance of Clostridium sensu stricto 1 and Romboutsia at T1, Prevotellaceae NK3B31 group, Rikenellaceae RC9 gut group, and Alloprevotella at T2, and p-2534-18B5 gut group at T3. Conversely, the NORM group exhibited increased concentrations of Ruminococcus at T1, HT002 at T2, and Prevotella_9 at T3. Furthermore, while plasma metabolite analysis revealed no significant differences between IUGR and NORM pigs at T1, decreased arginine levels were observed in the IUGR group compared with NORM at T2 (P &amp;lt; 0.05). In conclusion, our study underscores the profound and persistent impact of IUGR on fecal microbiota composition in pigs from birth to the initial stages of the finisher period, while suggesting minimal alterations in the blood metabolome profile throughout various growth stages. These findings provide valuable insights into the long-term consequences of IUGR on swine health and production. This research received funding from the European Union’s Horizon 2020 research and innovation program under a Marie Sklodowska-Curie grant (agreement no. 955374).

Dog ectoparasites as sentinels for pathogenic Rickettsia and Bartonella in rural Guatemala

Fleas and ticks serve as vectors of multiple pathogens in the genera Rickettsia and Bartonella that cause diseases in humans and other animals. Although human rickettsiosis and bartonellosis have been reported in all countries in Central America, limited research has been conducted to investigate the natural cycles of flea- and tick-borne rickettsiosis and bartonellosis, especially in Guatemala. We evaluated dog parasites as sentinels for zoonotic disease risk in rural Guatemala by sampling ticks and fleas from dogs, which were then identified and individually screened for Rickettsia and Bartonella. A total of 77 households were surveyed and 80.5 % of them had dogs. Overall, 133 dogs were examined for fleas and ticks, of which 68.4 % had fleas and 35.3 % had ticks. A total of 433 fleas and 181 ticks were collected from the infested dogs, with an additional 33 ticks collected from house walls. Three flea species were identified: Ctenocephalides felis (70.0 %), Echidnophaga gallinacea (11.8 %), and Pulex sp. (17.8 %). Among the collected ticks, 97 % were identified as Rhipicephalus sanguineus sensu lato with the rest being Amblyomma cajennense, A. auricularium, and A. ovale. Rickettsia felis were detected in six C. felis, in one Pulex sp., and in two R. sanguineus sensu lato, while Candidatus R. senegalensis was detected in one C. felis. Bartonella was detected only in fleas, including three Pulex sp. infected with B. vinsonii subsp. berkhoffii, B. henselae, and Bartonella sp., respectively, and 11 C. felis infected with B. henselae. This study reports Candidatus R. senegalensis and B. vinsonii subsp. berkhoffii in Guatemala for the first time, and indicates the potential risk of human and dog exposure to Rickettsia and Bartonella species. These results show that dogs provide critical information relevant to managing human potential exposure to flea- and tick-borne pathogens in rural Guatemala. This approach can potentially be expanded to other regions in Central America where domestic dogs are abundant and suffer from ectoparasite infestation.

Integrative taxonomy of Metarhizium anisopliae species complex, based on phylogenomics combined with morphometrics, metabolomics, and virulence data

Metarhizium anisopliae (Clavicipitaceae, Hypocreales) is a globally distributed entomopathogenic fungus, which has been largely studied and used in agriculture for its potent entomopathogenicity. Since its taxonomic establishment as a member of Metarhizium, many closely related taxa have been described with highly similar morphology (cryptic species). A holotype specimen of M. anisopliae is not extant, and the ex-neotype strain (CBS 130.71) does not form a monophyletic clade with other strains, up to now, recognized as M. anisopliae sensu stricto. In this study, we have conducted an integrative taxonomic treatment of M. anisopliae sensu lato by including the ex-neotype strain of M. anisopliae, other unknown strains from our collections identified as M. anisopliae s. lat., as well as other known species that have been previously delimited as closely related but distinct to M. anisopliae. By including whole-genome sequencing, morphometric analysis, LC–MS based metabolomics, and virulence assays, we have demonstrated that M. anisopliae s. str. should also include M. lepidiotae (synonym), and that M. anisopliae s. str. differentiates from the other species of the complex by its metabolome and less severe entomopathogenicity. New taxa, namely M. hybridum, M. neoanisopliae and M. parapingshaense spp. nov., are proposed. The novel taxa proposed here have strong phylogenomics support, corroborated by fine-scale differences in the length/width of conidia/phialides, while the metabolomics and virulence data still largely overlap. We have also demonstrated via population genomics data the existence of local clonal lineages, particularly the one corresponding to the persistence of a biocontrol candidate strain that has been used in the field application for three years. This study showcases the utility of combining various data sources for accurate delimitation of species within an important group of fungal biocontrol agents against pest insects.

A review of the Margarodidae sensu stricto (Hemiptera: Coccomorpha) in China, with descriptions of three new species

The research history of the family Margarodidae Cockerell sensu stricto (Hemiptera: Coccomorpha) from China is reviewed. Four species of Porphyrophora Brandt and three species of Neomargarodes Green from China are described and illustrated, including three new species: Neomargarodes chenopodium Zheng &amp; Wu, sp. nov. based on the morphology of the adult female, first-instar nymph and second-instar female and male nymphs (cysts); Porphyrophora ejinensis Zheng &amp; Wu, sp. nov. based on the adult female, second-instar female nymph (cyst) and third-instar female nymph (cyst); and Porphyrophora neimonggolensis Zheng &amp; Wu, sp. nov. based on the adult female. Porphyrophora ningxiana Yang, 1979, stat. rest. is resurrected from synonymy under Porphyrophora sophorae (Archangelskaya, 1935), and lectotypes of Neomargarodes gossypii Yang, 1979 and P. ningxiana Yang are designated. A record of Porphyrophora ussuriensis Borchsenius, 1949 from China was found to be based on a misidentification. An identification key to genera and species of Margarodidae s.s. found in China is provided, based on adult female morphology.