Pain Sensation Research Articles

Специфика психических состояний осужденных женщин, содержащихся в местах лишения свободы с малолетними детьми

Introduction. The aim of the empirical study was to research the mental states of convicted women with young children. The study sample consisted of 153 convicted women with young children serving their sentences in a correctional institution. The study was conducted on the basis of the State Department of the Federal Penitentiary Service of Russia for the Kemerovo Region, the Federal Penitentiary Service of Russia for the Moscow Region, the Federal Penitentiary Service of Russia for the Vladimir Region, the Federal Penitentiary Service of Russia for the Republic of Mordovia. The scientific problem is to study the mental states of convicted women with young children. Research methods and techniques. The following set of methods was used to study mental states: ‘16-factor personality questionnaire - 16 PF’, form C (R. Kettell), “Depression scale” (A. Beck), clinical questionnaire for identification and assessment of neurotic states (K. K. Yakhin, D. M. Mendelevich), methodology for determining the dominant mental state “Dominant state” (L. V. Kulikov), questionnaire for studying the level of aggression (A. Bass and A. Darkey). The results of the conducted research were the identification of mental states of convicted women in prison with young children. It was established that negative mental states (tension, emotional instability, frustration, anxiety, irritation, depression, anxiety) prevail among these women.They are expressed fatigue, hysteria, vegetative disorders, which are manifested in unpleasant or painful somatic sensations. A high level of aggression and hostility has been diagnosed. The revealed negative mental states need timely psychological correction.

Immediate effects of thoracic mobilization versus soft tissue release on trunk motion, pain, and lumbar muscle activity in patients with chronic low back pain

IntroductionThoracic mobilization and tissue release are common manual techniques in clinical practice. However, the relative effects of these two treatments on chronic low back pain (CLBP) have not yet been explored. Thus, this study aimed to investigate and compare the immediate effects of thoracic mobilization with those of soft tissue release on trunk movement, pain sensation, and muscle activity in patients with CLBP. Methods28 participants were randomly assigned to two intervention groups. The mobilization group received mobilization treatment at the hypomobile joints of the trunk segment, while the soft tissue release group received thoracolumbar fascia release and massage in the lumbar region. The trunk range of motion (ROM), tissue hardness, pressure pain threshold (PPT), and erector spinae activity during light-weight lifting tasks were measured before and immediately after both interventions. FindingsAll measured outcomes in both groups improved after intervention (p < 0.05). The mobilization technique significantly improved side bending and rotation, PPT, and tissue hardness compared to soft tissue release. However, lumbar muscle activation reduced to a greater extent in the soft-tissue release group. ConclusionBoth techniques improved trunk ROM and PPT and reduced tissue hardness and muscle activation. Therefore, both these techniques are recommended for patients with CLBP. Trial registrationISRCTN75190733.

Inhibition of TRPV1 by an antagonist in clinical trials is dependent on cholesterol binding

TRP Vanilloid 1 (TRPV1) channel, one of the major members of the TRP family was discovered to play a critical role in pain sensation, particularly inflammatory pain, and is associated with hyperalgesia, an enhanced sensitivity to pain. A new study by Fanet al.“Structural basis of TRPV1 inhibition by SAF312 and cholesterol” sheds new light on the mechanistic structural basis of TRPV1 inhibition by SAF312 (Libvatrep), a TRPV1 antagonist, currently in phase II clinical trials. They discover that the binding site of SAF312 in TRPV1 is in close vicinity and partially overlaps with the binding site of cholesterol and that removal of cholesterol interferes with the ability of SAF312 to suppress TRPV1 current.

PEMIJATAN TITIK LI-4 DAPAT MENGURANGI NYERI KANULASI ARTERIOVENOUSFISTULA PADA PASIEN CKD YANG MENJALANI HEMODIALISA: STUDI KASUS

Background, one of the vascular accesses used for hemodialysis is arteriovenous fistula. Arteriovenous fistula cannulation causes pain problems in patients. The cannulation procedure is felt as a painful sensation that needs to be treated, because the size of the needle used when cannulating an arteriovenous fistula is quite large compared to cannulating when installing an IV, namely 16 gauge. General purpose, to provide acute pain nursing care for Chronic Kidney Disease (CKD) patients undergoing hemodialysis in the hemodialysis room at RSU Aghisna Medika Kroya. Case collection method, pain scale measurement using a VAS instrument for 5 CKD patients undergoing hemodialysis without massage intervention and with massage intervention at the LI-4 point for 2 minutes or approximately 30-50 pressures. The results of nursing care, nursing care for acute pain in CKD patients undergoing hemodialysis after nursing for 1x4 hours with the application of massage at point LI-4 showed a decrease in the pain scale in patients without massage and with massage at point LI-4. In conclusion, LI-4 point massage therapy can be an therapeutic option to reduce the pain of arteriovenousfistula cannulation in CKD patients undergoing hemodialysis. Recommendation is hoped that more and larger case subjects will be taken in the next research so that the results will be more effective.

Navigating the Controversies: Role of TRPM Channels in Pain States.

Chronic pain is a debilitating condition that affects up to 1.5 billion people worldwide and bears a tremendous socioeconomic burden. The success of pain medicine relies on our understanding of the type of pain experienced by patients and the mechanisms that give rise to it. Ion channels are among the key targets for pharmacological intervention in chronic pain conditions. Therefore, it is important to understand how changes in channel properties, trafficking, and molecular interactions contribute to pain sensation. In this review, we discuss studies that have demonstrated the involvement of transient receptor potential M2, M3, and M8 channels in pain generation and transduction, as well as the controversies surrounding these findings.

Learned Symptom-Specific Fear Toward a Visceral Sensation and Its Impact on Perceptual Habituation.

Impaired habituation of bodily sensations has been suggested as a contributing factor to chronic pain. We examined in healthy volunteers the influence of fear learning toward a nonpainful sensation in the esophagus on the perceptual habituation of this sensation. In a homoreflexive fear learning paradigm, nonpainful electrical sensations in the esophagus were used as a conditioned stimulus (CS). This sensation was presented 42 times before, during, and after fear learning. In the fear learning group ( n = 41), the CS was paired with a painful electrical sensation in the esophagus (unconditioned stimulus [US]). In the control group ( n = 41), the CS was not paired with the US. Ratings for CS intensity, US expectancy, startle electromyogram (EMG), skin conductance responses (SCR), and event-related potentials (ERPs) to the CS were assessed. Compared to the control group, fear learning was observed in the fear learning group as evidenced by potentiated startle responses after the CS relative to ITI ( t (1327) = 3.231, p = .001) and higher US expectancy ratings ( t (196) = 3.17, p = .002). SCRs did not differ between groups ( F1,817 = 1.241, p = .33). Despite successful fear learning, the fear learning group did not show a distinct pattern of habituation to the visceral CS relative to the control group (intensity ratings: F1,77.731 = 0.532, p = .47; ERPs: F1,520.78 = 0.059, p = .94). Acquired fear to nonpainful esophageal sensations does not affect their perceptual habituation patterns.

Supraclavicular versus infraclavicular brachial plexus block in upper limb orthopaedic surgery: a systematic review and meta-analysis of randomised controlled trials.

Clavicular brachial plexus blocks are a popular method to provide analgesia in upper limb surgery. Two common approaches include the infraclavicular (IC) and supraclavicular (SC) blocks. These two techniques have been compared previously; however, it is still being determined from the current literature whether one should be favoured. A search was performed on the following databases: Ovid Medline, EMBASE and the Web of Science from inception until 30.04.2023. All RCTs comparing SC and IC approaches in upper limb orthopaedic surgery were included. The primary outcome was block success rate. Eighteen RCTs comprising 1389 patients were included. The success rate of IC blocks was higher than SC blocks, odds ratio 0.61 (95% CI 0.41-0.91, p = 0.01). A small number of studies reported on secondary outcomes. A reduced rate of Horner's syndrome was observed in the IC group. Otherwise, no difference was noted between the approaches in terms of procedure time, sensory onset time, patient satisfaction, pain and vascular puncture. IC blocks demonstrate a higher success rate over SC blocks. Across all studies a large variance in outcome reporting and definitions was observed. Future studies should conform to an agreed definition set to facilitate comparison.

Assessing the Use of BotulinumtoxinA for Hyperactive Urinary Tract Dysfunction a Decade after Approval: A Single-Blind Study to Evaluate the Reduction in Pain in OnabotulinumtoxinA Detrusor Injection Using Different Injection Needles.

Overactive bladder (OAB) has a significant impact on the quality of life; thus, it requires treatment that can be adhered to over a long period without undue side effects. The current treatment which uses an anticholinergic or β-3 agonist may fail to improve symptoms and has side effects, leading to high discontinuation rates. OnabotulinumtoxinA (OnabotA) detrusor injection has been approved for idiopathic OAB as a second-line treatment with good effectiveness and tolerability. This study used a visual analog scale (VAS) to assess the impact of the type of needle used for OnabotA detrusor injections under local anesthesia on the pain levels after each injection. This study included 68 female patients. We used three different needles with thicknesses ranging from 22 to 27 gauge, lengths between 4 and 5 mm, and different cuts. The sensation of pain was rated at each standardized injection location. Regardless of the needle used, the patients' perceptions of pain at the beginning of the procedure were rated as being less than the subsequent injections. Most pain sensations were rated as low to moderate. The mean pain sensation on the VAS was 2.5 ± 0.3 overall, i.e., for all patients and needles used. Statistically significant differences in pain sensation were rated only at some locations of the bladder (on the back wall and the right side of the bladder). The single needles averaged the following pain scores: 2.8 ± 0.3 for needle A (20 G, 4 mm), 2.1 ± 0.3 for needle B (27 G, 5 mm), and 2.6 ± 0.4 for needle C (20 G, 4 mm, sharp cut 15°). The 27-gauge needle caused significantly less pain, and it had no negative impact due to its length, which was 1 mm longer than the other needles. Thus, the needle thickness was a decisive factor in the patients' perceptions of pain.

Spectrum‐Based Topological Indices and Their QSPR Studies of Nonsteroidal Anti‐Inflammatory Drugs

ABSTRACTSpectrum‐based topological indices (eigenvalue‐based topological indices), a valuable tool for analyzing molecular structure. These topological indices are metrics that reflect the inherent characteristics of chemical substances, were employed in conjunction with quantitative structure‐property relationship (QSPR) to investigate nonsteroidal anti‐inflammatory drugs (NSAIDs) which are used toalleviate or eliminate pain sensations in affected areas. We have to use mathematica to compute various eigenvalue‐based indices and utilized statistical software to identify correlations between these indices and key physical properties of NSAIDs. The analysis revealed that specific indices, including index, index, index, and index exhibited strong associations with properties like complexity and refractivity, boiling point, polarity, and molar weight, respectively.

Bleomycin triggers chronic mechanical nociception by activating TRPV1 and glial reaction-mediated neuroinflammation via TSLP/TSLPR/pSTAT5 signals

Chronic pain is a universal public health problem with nearly one third of global human involved, which causes significant distressing personal burden. After painful stimulus, neurobiological changes occur not only in peripheral nervous system but also in central nervous system where somatosensory cortex is important for nociception. Being an ion channel, transient receptor potential vanilloid 1 (TRPV1) act as an inflammatory detector in the brain. Thymic stromal lymphopoietin (TSLP) is a potent neuroinflammation mediator after nerve injury. Bleomycin is applied to treat dermatologic diseases, and its administration elicits local painful sensation. However, whether bleomycin administration can cause chronic pain remains unknown. In the present study, we aimed to investigate how mice develop chronic pain after receiving repeated bleomycin administration. In addition, the relevant neurobiological brain changes after noxious stimuli were clarified. C57BL/6 mice aged five- to six-weeks were randomly classified into two group, PBS (normal) group and bleomycin group which bleomycin was intradermally administered to back five times a week over a three-week period. Calibrated forceps testing was used to measure mouse pain threshold. Western blots were used to assess neuroinflammatory response; immunofluorescence assay was used to measure the status of neuron apoptosis, glial reaction, and neuro-glial communication. Bleomycin administration induced mechanical nociception and activated both TRPV1 and TSLP/TSLPR/pSTAT5 signals in mouse somatosensory cortex. Through these pathways, bleomycin not only activates glial reaction but also causes neuronal apoptosis. TRPV1 and TSLP/TSLPR/pSTAT5 signaling had co-labeled each other by immunofluorescence assay. Taken together, our study provides a new chronic pain model by repeated intradermal bleomycin injection by activating TRPV1 and glial reaction-mediated neuroinflammation via TSLP/TSLPR/pSTAT5 signals.

Investigation on cognition for insomnia and preference for acupuncture in breast cancer survivors: a in-depth interview study

To understand the cognition for insomnia and preference for acupuncture in breast cancer survivors based on the in-depth interview. Thirty breast cancer survivors with insomnia symptoms were collected for in-depth interview. The interview questions included three aspects, i.e. sleep expectation, cognition for insomnia (discomfort caused by insomnia, and underlying inducing factors of insomnia) and the preference for acupuncture (treatment methods used in the past, the reasons for not choosing acupuncture, and the tendency of acupuncture treatment). Using Colaizzi content analysis method, the data was analyzed. Regarding sleep expectation, most breast cancer survivors with insomnia symptoms were able to maintain normal activity in daytime. Insomnia symptoms often led to fatigue, and the inducing factors of insomnia referred to the treatment with endocrine therapy, anticipatory anxiety and inadequate sleep hygiene. All of the patients had received pharmacotherapy. The use proportion of non-pharmacological therapies was relatively low, and acupuncture was not chosen due to "not familiar with" and "fear of pain". Concerning to the preference for acupuncture, patients preferred the therapeutic methods of acupuncture with mild pain sensation and gentle stimulation; and the treatment should be more acceptable if delivered 2 or 3 times a week. Breast cancer survivors have the expectations for sleep, and are willing to receive the treatment with medication for their sleep disorders. Because of lack of the knowledge for acupuncture effect on insomnia and fear of strong needling sensation, a part of patients are unwilling to be treated with acupuncture therapy, but they are expected to receive the treatment with acupuncture while feeling more comfortable.

Pruritus: An Overlooked Symptom of Spinal Tumors

Background Chronic itch is often associated with pain due to its complex nature. Neuropathic pruritus is defined as a debilitating form of chronic itching, about which not much is known to date. This condition may develop as a result of various neurological events and is associated with damage to the somatosensory nervous system, accounting for approximately 10% of chronic itching cases. Syringomyelia, transverse myelitis, radiculopathies, thoracic spine masses, brain tumors, strokes, and abscesses are among the conditions associated with neuropathic itching to date. Case presentation A 65-year-old female patient, with no known diseases and no regular medication usage, presented with a complaint of itching that had been ongoing for the past two years. Itching initially started in approximately five cm areas on the bilateral below-knee flexor surfaces. The patient, whose complaints persisted, was referred to the internal medicine outpatient clinic by dermatology. On physical examination, erythematous excoriated papules were observed in the areas affected by itching. The patient's blood sugar, liver and kidney function tests, complete blood count, erythrocyte sedimentation rate, thyroid function tests, urinalysis, stool parasitology were all normal or negative. The patient reported a sensation of coldness in the same area, was referred to neurosurgery to investigate the etiology of possible neuropathic itching. The patient's spinal imaging, revealed a spinal mass. She underwent surgery performed by a neurosurgeon, during which the spinal mass was completely removed. She reported that her itching had completely disappeared post-operatively. Conclusions The concept of itching as a variant of pain is not very new. Any damage occurring in the central nervous system or peripheral nervous system that affects the neurons responsible for transmitting and processing itch can lead to neuropathic itching. Focusing on spinal cord pathologies, any condition that damages the spinal cord may cause itching, depending on the level of damage. In cases of itching with dermatomal localization, where pain, hot or cold sensations, and paroxysmal itching are present, additional imaging methods or investigations for etiology should be performed.

Notalgia Paresthetica Dermatologist Report of Symptom Burden and Treatment: Results from a Physician Survey.

Notalgia paresthetica (NP) is a sensory neuropathy characterized by chronic pruritus, skin pain, and other pathologic sensations affecting the mid-to-upper back. NP may be under-recognized and under-diagnosed, with limited data available on its symptom presentation and treatment patterns. NP-DERM was an internet-based survey of dermatologists (n = 650) from 8 different countries on their perspectives on NP symptoms and current treatment practices. Dermatologists typically treated a median of 12 patients with NP per month. Dermatologists reported that itch (pruritus) was the most common symptom for their patients with NP, followed by hyperpigmentation and sensitive skin. The most burdensome NP symptom was pruritus, followed by burning or hot sensation, and painful or raw skin. The most prescribed treatments included non-medicated skin care, topical corticosteroids, oral antihistamines, medicated topicals, and gabapentin or pregabalin. Physicians reported low satisfaction with available treatments. The most common reason for physicians to discontinue patients' therapy was lack of response.

Keratinocyte-Derived Exosomes in Painful Diabetic Neuropathy.

Painful diabetic neuropathy (PDN) is a challenging complication of diabetes with patients experiencing a painful and burning sensation in their extremities. Existing treatments provide limited relief without addressing the underlying mechanisms of the disease. PDN involves the gradual degeneration of nerve fibers in the skin. Keratinocytes, the most abundant epidermal cell type, are closely positioned to cutaneous nerve terminals, suggesting the possibility of bi-directional communication. Exosomes are small extracellular vesicles released from many cell types that mediate cell to cell communication. The role of keratinocyte-derived exosomes (KDEs) in influencing signaling between the skin and cutaneous nerve terminals and their contribution to the genesis of PDN has not been explored. In this study, we characterized KDEs in a well-established high-fat diet (HFD) mouse model of PDN using primary adult mouse keratinocyte cultures. We obtained highly enriched KDEs through size exclusion chromatography and then analyzed their molecular cargo using proteomic analysis and small RNA sequencing. We found significant differences in the protein and microRNA content of HFD KDEs compared to KDEs obtained from control mice on a regular diet (RD), including pathways involved in axon guidance and synaptic transmission. Additionally, using an in vivo conditional extracellular vesicle (EV) reporter mouse model, we demonstrated that epidermal-originating GFP-tagged KDEs are retrogradely trafficked into the DRG neuron cell body. Overall, our study presents a potential novel mode of communication between keratinocytes and DRG neurons in the skin, revealing a possible role for KDEs in contributing to the axonal degeneration that underlies neuropathic pain in PDN. Moreover, this study presents potential therapeutic targets in the skin for developing more effective, disease-modifying, and better-tolerated topical interventions for patients suffering from PDN, one of the most common and untreatable peripheral neuropathies.

Traconia Fish Sting in a City without a Sea.

The traconia fish fish poisons people it comes into contact with using the spiny structure on its back. In this case, we report the exposure of a 62-year-old housewife to a dead traconia fish while cleaning the fish. The patient came to the emergency room with unbearable pain and burning sensation in his hand. He was not relieved by the analgesia given after the examination in the emergency room and the hot water immersion methods applied. Local anesthesia-analgesia was provided by the orthopedist using the regional block technique. It should not be forgotten that such rare cases may occur in places that are not connected to the sea and/or even when the fish is dead.

Actualización de la tricodinia, un reto para los dermatólogos

Trichodynia is the sensation of pain in the scalp, which, in most cases, is associated with certain types of alopecia. Despite being a term coined by Rebora back in 1996 to described patients with diffuse alopecia consistent with telogen effluvium, this symptom has currently been reported in other entities. Androgenic alopecia, scarring alopecia, alopecia areata, trichotillomania, and chemotherapy-induced alopecia are common causes of trichodynia. Similarly, its association with psychiatric comorbidities, including depression, anxiety, obsessive-compulsive disorder and somatoform disorders has been reported with a higher prevalence among women. Although its pathogenesis is still to be elucidate, some factors involved are substanceP, psychiatric comorbidities and perifollicular inflammation. Clinically it exhibits pain or discomfort of the scalp, almost always in association with hair los. The sensation of pain can occur throughout the scalp or locally in some specific areas. Diagnosis is clinical and one of exclusion. Regarding treatment, there are no specific therapies for trichodynia. However, the use of botulinum toxinA, antidepressants, neuromodulators, propranolol, topical corticosteroids, oral corticosteroids and topical cannabinoids are therapeutic alternatives that should be taken into consideration. Since treatment of trichodynia is still therapeutically challenging for dermatologists more prospective studies are needed to evaluate new therapies.

Effects of firefighters’ protective gloves on physiological responses, psychological responses, and manual performance in a cold environment

This study aimed to investigate the effects of firefighters' protective gloves on physiological responses, psychological responses, and manual performance in a cold environment through human trials. Twelve participants wearing firefighter protective equipment were exposed to a 16 °C environment, while their hands were exposed to a small chamber of 0 °C with (FPG) and without (CON) firefighting protective gloves. During the trials, physiological responses (core temperature (Tc), the mean skin temperature (Tsk), and heart rate (HR)), psychological responses (thermal sensation vote (TSV) and pain sensation vote (PSV)), and manual performance (handgrip strength, manual dexterity, maximum finger flexion, and tactile sensitivity)) were obtained. The results indicated a significant difference (p < 0.05) between FPG and CON regarding Tsk. Furthermore, pain sensation occurred when the mean skin temperature of the hand was between 15 °C and 20 °C. Gloves significantly (p < 0.05) reduced handgrip strength, manual dexterity, and tactile sensitivity in the cold exposure. This study provides fundamental knowledge for cold strain assessment and high-performance protective glove development with the potential to improve firefighters’ safety and health.

Оценка болевого синдрома в реабилитационных мероприятиях после операции по поводу разрыва ахиллова сухожилия

INTRODUCTION. Patients who have been injured need an early start of rehabilitation measures. This is due to the fact that prolonged restriction of motor activity, lack of exercise, vascular and other changes lead to muscle atrophy, limited mobility in joints, impaired metabolic processes in the extremities, loss of functional conditions. AIM. To optimize the complex of measures for early rehabilitation after surgery for Achilles tendon rupture, taking into account the assessment of pain syndrome. MATERIAL AND METHODS. The research work was carried out at the Department of Medical Rehabilitation and Sports Medicine. To implement the tasks set, the following stages were introduced: a critical analysis of domestic and foreign sources, the development of an individual rehabilitation plan with an assessment of pain syndrome. RESULTS AND DISCUSSION. For effective rehabilitation in the postoperative period, it is important to objectively control the patient's pain sensations, gradually increasing the load on the damaged tendon. The pain monitoring and weight load dosing scale based on the visual analog scale has found wide application. Visual analog scale consists of a horizontal line on which the extreme points correspond to 0 points (no pain) and 10 points (maximum unbearable pain). The patient marks a point on this line corresponding to his current pain level. The pain syndrome was assessed on the day of surgery, and then on the 7th, 14th, 21st, 35th, 42nd and 49th days of follow-up after surgery. If by the end of the second week after surgery, the pain syndrome is 0-2 points, this indicates a favorable healing process and the possibility to begin early mobilization of the tendon. During this period, you can gradually increase the weight load on the limb, starting with partial support on the foot. If the pain syndrome persists, it is more than 2 points according to visual analog scale by the end of the second week, which may indicate the development of complications that prevent normal healing: inflammation, failure of sutures, suppuration of the surgical wound. CONCLUSION. The proposed technique using a visual analog scale is a tool for assessing pain and functional status in the preparation of a set of measures for early rehabilitation after surgery for rupture of the Achilles tendon. The assessment of pain symptoms is a determining factor in the introduction of early tendon mobilization. KEYWORDS: Achilles tendon rupture, pain syndrome, early rehabilitation

Interferon-gamma Treatment of Human Umbilical Cord Mesenchymal Stem Cells can Significantly Reduce Damage Associated with Diabetic Peripheral Neuropathy in Mice.

Diabetic peripheral neuropathy causes significant pain to patients. Umbilical cord mesenchymal stem cells have been shown to be useful in the treatment of diabetes and its complications. The aim of this study was to investigate whether human umbilical cord mesenchymal stem cells treated with interferon-gamma can ameliorate nerve injury associated with diabetes better than human umbilical cord mesenchymal stem cells without interferon-gamma treatment. Human umbilical cord mesenchymal stem cells were assessed for adipogenic differentiation, osteogenic differentiation, and proliferation ability. Vonfry and a hot disc pain tester were used to evaluate tactile sensation and thermal pain sensation in mice. Hematoxylin-eosin and TUNEL staining were performed to visualize sciatic nerve fiber lesions and Schwann cell apoptosis in diabetic mice. Western blotting was used to detect expression of the apoptosis-related proteins Bax, B-cell lymphoma-2, and caspase-3 in mouse sciatic nerve fibers and Schwann cells. Real-Time Quantitative PCR was used to detect mRNA levels of the C-X-C motif chemokine ligand 1, C-X-C motif chemokine ligand 2, C-X-C motif chemokine ligand 9, and C-X-C motif chemokine ligand 10 in mouse sciatic nerve fibers and Schwann cells. Enzyme-linked immunosorbent assay was used to detect levels of the inflammatory cytokines, interleukin- 1β, interleukin-6, and tumor necrosis factor-α in serum and Schwann cells. The adipogenic differentiation capacity, osteogenic differentiation capacity, and proliferation ability of human umbilical cord mesenchymal stem cells were enhanced after interferon-gamma treatment. Real-Time Quantitative PCR revealed that interferon-gamma promoted expression of the adipogenic markers, PPAR-γ and CEBP-α, as well as of the osteogenic markers secreted phosphoprotein 1, bone gamma-carboxyglutamate protein, collagen type I alpha1 chain, and Runt-related transcription factor 2. The results of hematoxylin-eosin and TUNEL staining showed that pathological nerve fiber damage and Schwann cell apoptosis were reduced after the injection of interferon-gamma-treated human umbilical cord mesenchymal stem cells. Expression of the apoptosis-related proteins, caspase-3 and Bax, was significantly reduced, while expression of the anti-apoptotic protein B-cell lymphoma-2 was significantly increased. mRNA levels of the cell chemokines, C-X-C motif chemokine ligand 1, C-X-C motif chemokine ligand 2, C-X-C motif chemokine ligand 9, and C-X-C motif chemokine ligand 10, were significantly reduced, and levels of the inflammatory cytokines, interleukin-1β, interleukin-6, and tumor necrosis factor-α, were decreased. Tactile and thermal pain sensations were improved in diabetic mice. Interferon-gamma treatment of umbilical cord mesenchymal stem cells enhanced osteogenic differentiation, adipogenic differentiation, and proliferative potential. It can enhance the ability of human umbilical cord mesenchymal stem cells to alleviate damage to diabetic nerve fibers and Schwann cells, in addition to improving the neurological function of diabetic mice.

Health Education for Adolescent Girls in Overcoming Dismenorhea without Medication

Dysmenorrhea is pain in the lower abdomen during menstruation which is generally caused by contractions of the myometrium due to prostaglandin production. In Indonesia, 43-93% girls experiencing dysmenorrhea. When experiencing unbearable menstrual pain, many women need medication to relieve it. However, the use of drugs often has side effects on the body. One of the non-pharmacological management methods that can be used is relaxation techniques that inhibit the brain from producing pain sensations and do not cause side effects for the body. The purpose of the community service activities carried out at Senior High School 1 Kandat, Kediri Regency is to provide education about non-pharmacological therapy methods in reducing the intensity of dysmenorrhea pain that are easy to apply, especially to adolescent girls when experiencing menstrual pain. The population of this community service is female students. The implementation of this community service activity is by providing health education on how to overcome menstrual pain without medication. The result of this activity was an increase in the level of knowledge of 85% of female students on how to overcome menstrual pain without medication. This activity concludes that it was implemented and resulted in changes by the target output, namely knowledge on overcoming menstrual pain without medication. It is hoped that the counseling provided can be implemented routinely and applied.