Sensitive Profile Research Articles

Investigations of Antibiotic Susceptibilities of S. aureus Strains Isolated from Various Clinical Samples

Objective: Staphylococcus aureus (S. aureus) is a critical microorganism that causes a range of infections with high morbidity and mortality rates, including skin and soft tissue infections, urinary tract infections, endocarditis, pneumonia, septic arthritis, osteomyelitis, and sepsis in both community and healthcare settings. The objective of this study was to ascertain the antimicrobial resistance rates of methicillin-resistant S. aureus (MRSA) and Methicillin-susceptible S. aureus (MSSA) isolates derived from a range of clinical samples submitted to the Medical Microbiology Laboratory of our hospital and to examine the resistance profile specific to our hospital. Methodology: The study included 229 S. aureus isolates collected between 2022 and 2023. The isolates were identified through the application of conventional methods and the MALDI-TOF-MS system (VITEK MS, bioMerieux France). The antimicrobial susceptibility of the isolates was determined by the BD Phoenix automated system (Becton Dickinson, USA) in accordance with the criteria set forth by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: The MRSA rate in the one-year period was 25.77%. When the distribution of S. aureus isolates was analyzed, it was determined that blood cultures were the most common clinical specimens from which S. aureus was isolated. Resistance to glycopeptides and linezolid was determined in MRSA isolates, albeit at a low rate. No resistance to glycopeptide and linezolid was detected in MSSA isolates. MSSA isolates were found to have a more sensitive profile to other antibiotics than MRSA isolates. The highest resistance rate was detected against penicillin with 100% and 87.06% in MRSA and MSSA isolates, respectively. In addition, the most sensitive antibiotics were determined to be glycopeptide, linezolid, daptomycin, and aminoglycoside. Conclusion: In conclusion, knowing the resistance profiles of S. aureus isolates in our hospital, will guide empirical treatment. Furthermore, the implementation of effective infection control measures and a cautious approach to antibiotic use will contribute to the management of MRSA infections.

Fabrication of Famotidine Loaded Sepiolite/Pectin Bionanocomposite Hydrogels for Controlled Drug Delivery

AbstractDevelopment of drug delivery systems is an extensively researched area in the biomedical field. In recent years, there has been an increasing interest in fabrication of biocompatible nanofibrous drug delivery systems. The present study describes the fabrication of calcium chloride‐crosslinked bionanocomposite hydrogels based on pectin for the use of gastro‐retentive delivery system. Sepiolite/famotidine/pectin nanocomposite hydrogel was fabricated by using Ionotropic gelation process. The thermal, structural and morphological properties were evaluated via different experimental techniques such as TGA‐DSC, FTIR, and SEM. The prepared hydrogels showed a pH sensitive swelling profile with maximum water absorption at pH 1.2. Analysis of in‐vitro drug release data revealed that the sepiolite/famotidine/pectin nanocomposite hydrogels exhibited a slight initial burst release, followed by a sustained release over a 24 h period. This was observed both in simulated gastric fluid and in phosphate‐buffered saline, suggesting that these hydrogels are well‐suited for prolonged drug delivery. To investigate the drug release kinetics, various models were applied. The release of famotidine was predominantly governed by diffusion, as described by the Korsmeyer‐Peppas model.

Contextually Sensitive Polyvictimization Profiles and Physical and Mental Health Outcomes among South African Adolescents from Low-Resource Communities: An Extended Latent Class Analysis

Adolescents in South Africa may experience violence victimizations in many forms (abuse, bullying, assault) and across many contexts (home, school, community). Polyvictimization is a valuable framework to examine the diversity of violence in adolescents’ lives, particularly when employed alongside person-centered methods such as latent class analysis. This study builds on previous work examining contextually sensitive patterns of polyvictimization among South African adolescents and uses longitudinal Young Carers data (n = 3401) from highly-deprived, low-resource settings to investigate the associations between latent class polyvictimization profiles and physical and mental health outcomes using the Bolck–Croon–Hagenaars (BCH) method. Key results found that adolescents who experienced high polyvictimization alongside contextual factors (poverty, disability, etc.) had greater odds of suicidality and higher scores for depression and anxiety measures than their peers who experienced moderate or low polyvictimization. These outcomes were often worse within the high polyvictimization classes for those participants experiencing burdens of HIV/AIDS and disability. Results were mixed for the physical health outcomes (chronic health condition; recent poor health) and when distinguishing between the moderate and low polyvictimization classes, as some classes characterized by decreased polyvictimization had worse health outcomes. These findings highlight the need to consider polyvictimization as a relevant health risk and contextual factor when addressing the health and well-being needs of South African adolescents. Health programming and policy efforts should seek to screen for and address the causes of and distress from polyvictimization when targeting adolescents and provide a cohesive response to the adolescent violence and health burdens in low-resource communities.

Machine learning-based pathomics signature of histology slides as a novel prognostic indicator in primary central nervous system lymphoma

PurposeTo develop and validate a pathomics signature for predicting the outcomes of Primary Central Nervous System Lymphoma (PCNSL).MethodsIn this study, 132 whole-slide images (WSIs) of 114 patients with PCNSL were enrolled. Quantitative features of hematoxylin and eosin (H&E) stained slides were extracted using CellProfiler. A pathomics signature was established and validated. Cox regression analysis, receiver operating characteristic (ROC) curves, Calibration, decision curve analysis (DCA), and net reclassification improvement (NRI) were performed to assess the significance and performance.ResultsIn total, 802 features were extracted using a fully automated pipeline. Six machine-learning classifiers demonstrated high accuracy in distinguishing malignant neoplasms. The pathomics signature remained a significant factor of overall survival (OS) and progression-free survival (PFS) in the training cohort (OS: HR 7.423, p < 0.001; PFS: HR 2.143, p = 0.022) and independent validation cohort (OS: HR 4.204, p = 0.017; PFS: HR 3.243, p = 0.005). A significantly lower response rate to initial treatment was found in high Path-score group (19/35, 54.29%) as compared to patients in the low Path-score group (16/70, 22.86%; p < 0.001). The DCA and NRI analyses confirmed that the nomogram showed incremental performance compared with existing models. The ROC curve demonstrated a relatively sensitive and specific profile for the nomogram (1-, 2-, and 3-year AUC = 0.862, 0.932, and 0.927, respectively).ConclusionAs a novel, non-invasive, and convenient approach, the newly developed pathomics signature is a powerful predictor of OS and PFS in PCNSL and might be a potential predictive indicator for therapeutic response.

Developmentally sensitive multispectral cortical connectivity profiles serving visual selective attention

Throughout childhood and adolescence, the brain undergoes significant structural and functional changes that contribute to the maturation of multiple cognitive domains, including selective attention. Selective attention is crucial for healthy executive functioning and while key brain regions serving selective attention have been identified, their age-related changes in neural oscillatory dynamics and connectivity remain largely unknown. We examined the developmental sensitivity of selective attention circuitry in 91 typically developing youth aged 6 – 13 years old. Participants completed a number-based Simon task while undergoing magnetoencephalography (MEG) and the resulting data were preprocessed and transformed into the time-frequency domain. Significant oscillatory brain responses were imaged using a beamforming approach, and task-related peak voxels in the occipital, parietal, and cerebellar cortices were used as seeds for subsequent whole-brain connectivity analyses in the alpha and gamma range. Our key findings revealed developmentally sensitive connectivity profiles in multiple regions crucial for selective attention, including the temporoparietal junction (alpha) and prefrontal cortex (gamma). Overall, these findings suggest that brain regions serving selective attention are highly sensitive to developmental changes during the pubertal transition period.

A Rare Agent in Wound Infection: Leclercia adecarboxylata

As a rare infectious agent, Leclercia adecarboxylata is a gram-negative bacillus belonging to the Enterobacteriaceae family. It can be isolated from human blood, sterile body fluids, sputum, urine, fecal, and wound samples. In this case report, the isolation of L. adecarboxylata as a causative agent in the wound and tissue sample cultures of a soft tissue infection that developed after arthrodesis surgery on the right foot of a 47-year-old female patient who was followed up with Charcot foot treatment due to diabetes in our hospital is presented. The patient's general condition was good, his leukocyte count was 6000/ml, leukocyte distribution was 58% neutrophil predominance, erythrocyte sedimentation rate was 105 mm/hour, and C-reactive protein level was 90 mg/L. Wound and tissue samples from the area considered to be the focus of infection were sent to our laboratory for culture examination. In the samples inoculated into routine identification media and subjected to standard incubation and identification procedures, a growth consisting of pure colonies (monomicrobial) was detected on the culture media. The isolate was identified as L. adecarboxylata by colony morphology, microscopic examinations (gram-negative bacilli), other basic identification procedures, and mass spectrometry (MALDI-TOF MS). Since it is a rare infectious agent, additional biochemical tests were performed; glucose-sucrose-lactose utilization, motility, indole production, and esculin hydrolysis tests were found to be positive, while oxidase, ornithine decarboxylase, hydrogen-sulfide production, citrate utilization, and urease tests were found to be negative. Antibiotic susceptibility tests were performed with the VITEK 2® automated system (Biomérieux, France), and the results were evaluated according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) v13.0 criteria. The isolate, which has a generally sensitive antibiotic profile, was evaluated as the infectious agent and the patient was treated with intravenous ampicillin/sulbactam for five days. The patient, whose laboratory values tended to improve (leukocyte count 4400/ml, neutrophil rate 52.9%), was discharged with peroral amoxicillin/clavulanic acid treatment.

Behavior Rating Inventory of Executive Function in Preschool (BRIEF-P) and Attention-Deficit and Hyperactivity Disorders (ADHD): A Systematic Review and Meta-Analysis of Floor and Ceiling Effects.

ADHD is a neurodevelopmental disorder that is accompanied by executive challenges. To obtain evidence of the usefulness of the BRIEF-P and to analyze the possible ceiling and floor effect of its scores in the assessment of executive function in preschoolers with signs compatible with a possible diagnosis of ADHD. A search was performed in Science Direct, NCBI (PubMed), and ProQuest Education Journals during the period 2012-2022. We included studies that evaluated samples of individuals with symptomatology compatible with ADHD, with an age range between 2 and 6 years, published in English or Spanish. Of a total of 2538 articles, only seven met the inclusion criteria. The risk of bias was assessed using the QUADAS-2 questionnaire. The main variables were age and executive functioning. Executive deficits in early-age individuals with symptoms compatible with ADHD are more extensive than just deficits in working memory. A floor effect has been found in tests associated with hot executive functions and a ceiling effect in cold executive functions. This makes it necessary to use different tests to assess executive performance in preschoolers with ADHD-compatible symptomatology and to design intervention proposals accordingly. The BRIEF-P is an instrument that facilitates obtaining a sensitive and discriminative executive profile, although it should be used in combination with other neuropsychological performance tests.

Assessing biases in atmospheric parameters for radiative effects estimation in tropical regions

Many studies highlight the importance of water vapor in tropical atmospheric radiative transfer calculations. However, limited research focuses on understanding the effects of biases in different atmospheric factors, such as pressure, temperature, water vapor, and ozone densities. These biases stem from various sources, including satellites, reanalysis data, and the standard TROPICAL atmosphere. This study explores how these biases influence estimates of two key things: atmospheric radiative forcing (ARF) and heating rate (HR) profiles over the tropical region. The study is undertaken at Gadanki, a rural background site in the tropics. It considers different situations - clear skies and those with aerosols - across different parts of the electromagnetic spectrum and throughout the seasons. Additionally, this study investigates how sensitive ARF and HR profiles are to biases in each of these atmospheric factors within specific spectral ranges. The study results show that the standard TROPICAL atmosphere often differs significantly from real-time measurements, leading to varied effects on radiation. Among the various factors, biases in water vapor density have the most substantial impact on ARF and HR, followed by biases in temperature. Moreover, longwave radiation is more sensitive to water vapor and temperature changes. This study emphasizes the importance of considering the exact state of the atmosphere, especially in tropical regions, when estimating ARF and HR. To improve estimates when real-time data isn't available, the study suggests combining data from the Microwave Limb Sounder (MLS) and the Atmospheric Infrared Sounder (AIRS), and using reanalysis data for broader assessments. It's also important to be cautious when using the TROPICAL atmosphere to analyze the radiative effects of aerosols.

CRISPR/Cas9 Screen Identifies CPX-351 and 7+3 Regimens Response Modulators with Distinct Sensitive and Resistant Profiles

CPX-351 (dual-drug liposomal encapsulation of daunorubicin and cytarabine in a synergistic 1:5 molar ratio) is approved for newly diagnosed high-risk and secondary AML as an alternative to the widely used cytarabine and daunorubicin (7+3) regimen. Despite newly approved drugs, many AML patients still developed treatment resistance and relapse, leading to poor survival. Amid mounting research into the molecular mechanisms of AML therapies, treatment resistance remains a major concern. To overcome treatment resistance, it is necessary to examine the molecular mechanisms underlying the CPX-351 and 7+3 treatment response. We conducted custom CRISPR/Cas9 synthetic-lethal screens targeting 2440 genes (AML-relevant genes, cytarabine and daunorubicin pharmacology, and druggable genes) in six AML cell lines, profiled drug-sensitive/-resistant modulators, and identified novel targets for overcoming drug resistance in CPX-351 and 7+3 regimen. Six AML cell lines were transduced with a custom library and puromycin was selected before pool transduced cells were treated with DMSO, the IC30-IC50 of cytarabine, daunorubicin, or CPX-351 for 7 doubling-times at 500x coverage. All samples were processed with genomic DNA, sgRNAs were amplified by PCR, and Illumina NovaSeq 6000 SP 100SR sequencing was performed. The abundance of sgRNA was analyzed using MAGeCK-RRA to estimate the RRA drug response score (Drug vs. Control conditions at 7-doubling times) or RRA essential score (Control at 7-doubling time vs. Control at Day 0). Genes with an average RRA score of &amp;lt; -1 across six AML cell lines were defined as drug-resistant or essential genes (negative selection), whereas genes with an average RRA score of &amp;gt; 1 were categorized as sensitive or suppressor genes (positive selection). Next, comparisons of functional pathways with over-representation analysis were performed on resistant and sensitive genes across three drugs to determine differences in their functions. At the average RRA response score cut-off, 61, 163, and 112 were identified as drug-resistant genes, while 46, 93, and 86 were sensitive genes to cytarabine, daunorubicin, or CPX-351 (provided by Jazz Pharmaceuticals), respectively. Fig 1A showed a Venn diagram of all significant resistant and sensitive genes across all three drugs with 14 common significant genes and 73, 174, and 118 unique genes to cytarabine, daunorubicin and CPX-351, respectively, along with their essential score. Among these significant common response genes, some notable genes included ABCC1, SAMHD1 and TP53, where CRISPR knocking out screens led to depletion or enrichment of sgRNA when treated with drugs across all AML cell lines. ABCC1 is a drug-resistant gene that encodes the ATP Binding Cassette C1-mediated drug efflux transporter; therefore, knocking out the efflux transporter causing drug resistance, would increase the lethality of AML cells . Our result also showed ABCC1 isa tumor suppressor gene; because knocking out the ABCC1 gene improves cell survival and growth . SAMHD1 is another notable gene that encodes for deoxynucleoside triphosphate (dNTP) tri-phosphohydrolase that cleaves physiological dNTPs into deoxyribonucleosides and inorganic triphosphate. SAMHD1 has been shown to cause resistance in cytarabine, but there is limited evidence of its response to other drugs. In this study, we showed that SAMHD1 is a resistant gene for CPX-351 and a sensitive gene for daunorubicin, and it is a tumor suppressor gene. Lastly, TP53 is one of the significant common sensitive genes to cytarabine, daunorubicin, and CPX-351. TP53 encodes for the well-known tumor suppressor gene tumor protein 53, and mutations in TP53 have been shown to affect drug response. In this study, we demonstrated that knocking out TP53 in all AML cell lines contributed to cell survival and growth as a tumor suppressor gene and a drug-sensitive gene across the three drugs. Comparing significant resistant and sensitive genes across CPX-351, cytarabine, and daunorubicin, as shown in Fig 1B, revealed that resistant genes shared some common involvement in the cell cycle and cellular response to stress, but resistant genes for each drug displayed more distinct functional pathways. Moreover, each drug's sensitive genes revealed distinct functional enrichment pathways. Our current research is focused on identifying novel drug targets to combat drug resistance in the CPX-351 and 7+3 regimens.

Experience with the interleukin 6 inhibitor (Olokizumab) in patients with rheumatoid arthritis in real clinical practice: Influence on inflammatory activity and disease outcomes

The aim of the study was to evaluate in real clinical practice the effectiveness of therapy with an interleukin-6 inhibitor (olokizumab) in patients with rheumatoid arthritis (RA) in terms of clinical and laboratory activity of the disease, as well as patient-reported outcomes (PROs). Material and methods. 10 patients with a reliable diagnosis of RA were examined: the average age of the patients was 45.70±17.9 years, the duration of the disease was 9.0 (3.0; 12.0) years. Patients were with moderate or high disease activity: DAS28-ESR – 5.13 (4.34; 5.80) points; CDAI – 30.00 (24.00; 35.00); SDAI – 31.86 (24.36; 38,59). All patients were prescribed treatment with olokizumab (OKZ) at a dose of 64 mg subcutaneously every 4 weeks against the background of therapy with methotrexate, leflunomide, non-steroidal anti-inflammatory drugs and glucocorticoids (GC) (up to 10 mg/day in terms of prednisolone). Three patients had previously received tocilizumab (intravenously once a month at a dose of 8 mg/kg), the administration of which was discontinued for administrative reasons 6–12 months before the appointment of OKZ. The results of treatment were assessed by the dynamics of clinical, laboratory parameters (DAS28-ESR, SDAI, СDAI, CRP, ESR, IgM RF, ACCP) and outcomes assessed by the patients themselves (PROs): HAQ-DI index, general assessment of the health status of patients (OSZB) according to VAS, pain according to VAS; scales FACIT, SF-36. As psychometric methods, the questionnaire “Type of attitude towards the disease (TOBOL)”, the Hospital Anxiety and Depression Scale (HADS), and the Toronto Alexithymic Scale (TAS-26) were used. Observation was carried out before treatment, after 3 and 6 months of therapy. Results. Against the background of OKZ therapy, after 3 and 6 months, compared with the baseline, there was a significant decrease in the clinical indices of RA activity: DAS28-ESR – 5.13 (4.34; 5.80), 3.53 (2.83; 4.26) and 3.48 (2.8; 4.10) points respectively; CDAI – 30.00 (24.00; 35.00), 11.00 (6.0; 16.00) and 10.0 (5.0; 15.0) points respectively; SDAI – 31.86 (24.36; 38.59), 11.05 (6.07; 16.07) and 10.17 (7.02; 15.02) points respectively; CRP – 14.30 (7.00; 24.70), 0.70 (0.40; 0.90) and 0.65 (0.20; 3.0) mg/l respectively. No significant dynamics of ESR, RF IgM and ACCP was noted. After 3 and 6 months of treatment with OKZ, there was a significant decrease in OSZB and pain severity according to the VAS scale, and an improvement in the functional state of patients was observed according to the HAQ-DI questionnaire of fatigue indicators (FACIT-F) (p&lt;0.05). The physical component of the SF-36 scale increased significantly only by the 6th month of therapy (p&lt;0.01), while the mental component did not undergo significant changes (p&gt;0.05). In the process of treatment of OKZ, the attitude of patients to the disease changed from the ergopathic, neurasthenic and sensitive components in the TOBOL profile, before it began, then by its end, the dominant ones were harmonious, ergopathic and sensitive profiles. In addition, starting from the 3rd month of treatment in patients with RA, an adaptive response to the disease prevailed. A decrease in the level of anxiety was revealed, compared with the baseline, after 3 and 6 months of observation, depression indicators did not change significantly. Conclusion. In general, the results of this study indicate the effectiveness of ICD in RA, not only in terms of reducing the clinical and laboratory activity of the disease, but also in terms of outcomes reported by the patient himself, characterizing the quality of life and the psycho-emotional state of patients.

Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation.

Cis-regulatory elements (CREs) can be classified by the shapes of their transcription start site (TSS) profiles, which are indicative of distinct regulatory mechanisms. Massively parallel reporter assays (MPRAs) are increasingly being used to study CRE regulatory mechanisms, yet the degree to which MPRAs replicate individual endogenous TSS profiles has not been determined. Here, we present a new low-input MPRA protocol (TSS-MPRA) that enables measuring TSS profiles of episomal reporters as well as after lentiviral reporter chromatinization. To sensitively compare MPRA and endogenous TSS profiles, we developed a novel dissimilarity scoring algorithm (WIP score) that outperforms the frequently used earth mover's distance on experimental data. Using TSS-MPRA and WIP scoring on 500 unique reporter inserts, we found that short (153 bp) MPRA promoter inserts replicate the endogenous TSS patterns of ∼60% of promoters. Lentiviral reporter chromatinization did not improve fidelity of TSS-MPRA initiation patterns, and increasing insert size frequently led to activation of extraneous TSS in the MPRA that are not active in vivo. We discuss the implications of our findings, which highlight important caveats when using MPRAs to study transcription mechanisms. Finally, we illustrate how TSS-MPRA and WIP scoring can provide novel insights into the impact of transcription factor motif mutations and genetic variants on TSS patterns and transcription levels.

Ultimate Bearing Capacity Calculation of Soil With Finite Element Method

Ultimate bearing capacity calculation is one of the most important factor to design foundations in geotechnical engineering. In this paper, ultimate bearing capacity values were calculated with finite element and analytical methods and compared. A case study of Bozozuk (1972) article is used for ultimate bearing capacity evaluations. On 30 September 1970, 600-ton weight concrete silo suddenly overturned and failure. After failure, in-situ Vane shear test and laboratory tests carried out to determine soil strength parameters for back analysis. In finite element analysis, Plaxis 2D was use. From analytical method maximum factor of safety FS= 1,09 from Terzaghi (1943) and minimum factor of safety FS= 0,97 from Meyerhof (1963). According to finite element analysis results, factor of safety was calculated as FS=0,64. All these results indicate that especially for complex and sensitive soil profiles, finite element analysis method may be used instead of analytical method.

Identification of sequence mutations in Phytophthora cactorum genome associated with mefenoxam resistance and development of a molecular assay for the mutant detection in strawberry (F. × ananassa)

Phytophthora crown rot (PhCR) caused by Phytophthora cactorum is one of the most damaging diseases of strawberry worldwide. Mefenoxam is one of the major fungicides currently used to manage PhCR. However, the emergence and spread of resistant isolates have made controlling the pathogen in the field problematic. In the present study, using whole genome sequencing analysis, mutations associated with mefenoxam-resistant isolates were identified in six different genomic regions of P. cactorum. The 95.54% reads from a sensitive isolate pool and 95.65% from a resistant isolate pool were mapped to the reference genome of P. cactorum P414. Four point mutations were in coding regions while the other two were in noncoding regions. The genes harboring mutations were functionally unknown. All mutations present in resistant isolates were confirmed by sanger sequencing of PCR products. For the rapid diagnostic assay, SNP-based high-resolution melting (HRM) markers were developed to differentiate mefenoxam-resistant P. cactorum from sensitive isolates. The HRM markers R3-1F/R3-1R and R2-1F/R2-1R were suitable to differentiate both sensitive and resistant profiles using clean and crude DNA extraction. None of the mutations associated with mefenoxam resistance found in this study were in the RNA polymerase subunit genes, the hypothesized target of this compound in oomycetes. Our findings may contribute to a better understanding of the mechanisms of resistance of mefenoxam in oomycetes since serves as a foundation to validate the candidate genes as well as contribute to the monitoring of P. cactorum populations for the sustainable use of this product.

A Study on the Retrieval of Ozone Profiles Using FY-3D/HIRAS Infrared Hyperspectral Data

Atmospheric ozone is a pollutant gas that has an important influence on the process of atmospheric radiation transmission and climate change. The Fengyun-3D (FY-3D) satellite Hyperspectral Infrared Atmospheric Sounder (HIRAS) has better spectral performance than other remote sensing payloads. Its observation radiation data contains abundant atmospheric vertical information, which can be used for ozone retrieval, but there are no ozone profile business products being generated at present. Therefore, for the mainland of Hong Kong, based on HIRAS infrared hyperspectral observation data, we used the traditional one-dimensional variational (1D-VAR) physical retrieval algorithm, combined with the radiative transfer model for TOVS (RTTOV), and selected the spectrum channel according to the optimal sensitive profile algorithm. The artificial neural network (ANN) algorithm was used to optimize the prior profiles, and the atmospheric ozone profile retrieval system was established. Finally, a set of ozone profile retrieval schemes suitable for FY-3D/HIRAS were summarized. We used ERA5 reanalysis data and World Ozone and Ultraviolet Radiation Data Centre (WOUDC) data to determine true values. The retrieval results were compared with Global Forecast System (GFS) forecast data, Ozone Mapping and Profile Suite (OMPS) ozone products, and Atmospheric Infrared Sounder (AIRS) ozone products. The results show that our ozone profile retrieval scheme makes up for the shortcomings of the conventional physical methods in some atmospheric pressure levels. The overall root-mean-square error (RMSE) of the ozone from the ground to the top of the stratosphere is within 30% on average, which was better than that for the GFS forecast data; the retrieval accuracy RMSE (%) was less than 20% in the pressure layer with the highest ozone concentration (15–25 hPa), which is better than that of OMPS ozone products and AIRS ozone products. The retrieval results prove that FY3D/HIRAS observation data allow ozone profile retrieval. This paper provides a reference for generating independent HIRAS ozone profile product data sets in business, and provides support for the subsequent application of Fengyun-3 series meteorological satellites in atmospheric parameter remote sensing.

Lebanese Consumer Perception towards Moringa Oleifera : Sensory Evaluation of Moringa Oleifera Fortified Labneh

Labneh is one of the most consumed dairy products in the Lebanese community, on breakfast, as a snack or on dinner, plane or mixed with herbs. Its various health benefits plus its acceptability among people makes it an important part of the Lebanese diet. In both its synthetic and homemade forms, several additives are usually incorporated to enhance its flavor and offer a variety of tastes. The problem with Labneh is its short shelf life, as well as its sensitive sensory profile. That is why most industries mix chemical additives with Labneh to prolong its shelf life and conserve its taste. The aim of this research is to preserve Labneh naturally by adding Moringa Oleifera dried leaves to extend its shelf life and inhibit rapid spoilage. Working on a natural preservation of Labneh instead of chemical preservation will be assessed by the survey’s results and prepare for studying the overall acceptability of the product by making a sensory analysis. The results of the questionnaire showed that the Lebanese community which is not totally aware of Moringa Oleifera benefits and availability, is willing to try fortified Labneh with Moringa as a new product with extended shelf life and more health benefits. The results of the sensory analysis showed that the sensory attributes of the samples with Moringa after 21 days of storage, where not significantly different than normal plane Labneh, which indicate that adding Moringa did not affect the sensory attributes of Labneh giving it more acceptability to consumers.

Efficient improvement in chemo/photothermal synergistic therapy against lung cancer using Bi@Au nano-acanthospheres

Novel highly hydrophilic and biocompatible bismuth nanospheres with gold nanoparticles growing outside (Bi@Au nano-acanthospheres, Bi@Au NASs) were synthesized through a simple procedure, which demonstrated to be a promising photothermal agent owing to the ultrahigh photothermal conversion efficiency (η = 46.6 %). The as-prepared Bi@Au NASs showed excellent blood compatibility and fairly low cytotoxicity to human lung cancer A549 cells, as well as efficient photothermal ablation (PTA) therapy induced by a near-infrared laser. Under the 808 nm laser radiation, the tumour temperature could be elevated by ∼25 °C high enough to kill the cancer cells. Moreover, the anticancer drug doxorubicin hydrochloride (DOX) was successfully loaded in Bi@Au NASs with a loading content as high as 16.78 % and released under a pH sensitive release profile, a characteristic beneficial for intravenous delivery of DOX into cancer cells for chemotherapy. The presence of the Bi element enabled Bi@Au NASs to act as a favourable computed tomography (CT) contrast medium for CT imaging-guided tumour treatment. Compared with cancer treatment through either photothermal therapy or chemotherapy, the chemo-photothermal synergistic therapy using Bi@Au NASs as both a photothermal agent and a drug carrier has efficiently enhanced the in vitro and in vivo therapeutic effects in cancer treatment.

Securing IoT Devices by Exploiting Backscatter Propagation Signatures

The low-power radio technologies open up many opportunities to facilitate Internet-of-Things (IoT) into our daily life, while their minimalist design also makes IoT devices vulnerable to many active attacks. Recent advances use an antenna array to extract fine-grained physical-layer signatures to identify the attackers, which adds burdens in terms of energy and hardware cost to IoT devices. In this paper, we present ShieldScatter, a lightweight system that attaches low-cost tags to single-antenna devices to shield the system from active attacks. The key insight of ShieldScatter is to intentionally create multi-path propagation signatures with the careful deployment of tags. These signatures can be used to construct a sensitive profile to identify the location of the signals’ arrival, and thus detect the threat. In addition, we also design a tag-random scheme and a multiple receivers combination approach to detect a powerful attacker who has the strong priori knowledge of the legitimate user. We prototype ShieldScatter with USRPs and tags to evaluate our system in various environments. The results show that even when the powerful attacker is close to the legitimate device, ShieldScatter can mitigate 95 percent of attack attempts while triggering false alarms on just 7 percent of legitimate traffic.

Application of T-cell receptor repertoire as a novel monitor in dynamic tracking and assessment: A cohort-study based on RA patients.

T-cell receptor repertoire (TCRR) sequencing has been widely applied in many fields as a novel tool. This study explored characteristics of TCRR in detail with a cohort of 598 rheumatoid arthritis (RA) patients before and after anti-rheumatic treatments. We highlighted the abnormal TCRR distribution in RA characterized by decreased diversity and increased proportion of hyperexpanded clones (HECs), which was potentially attributed to skewed usage of global V/J segments but not a few certain ones. Enriched motifs analysis in RA community demonstrated the huge heterogeneity of CDR3 sequences, so that individual factors are strongly recommended to be taken into consideration when it comes to clinical application of TCRR. Disease-modifying antirheumatic drugs (DMARDs) can regulate immune system through recovery of TCRR richness to relieve symptoms. Remarkably, sensitive gene profile and advantageous gene profile were identified in this study as new biomarkers for different DMARDs regimens.

Synthesis of magnetic/pH dual responsive dextran hydrogels as stimuli-sensitive drug carriers

Hydrogels loaded with magnetic nanoparticles have been widely researched recently as biomaterials, due to their good biocompatibility and unique magnetic characteristics. In this study, water-soluble superparamagnetic iron oxide nanoparticles (Fe3O4) prepared by coprecipitation were physically doped into the dextran hydrogels which were formed via Schiff base reactions between ethylenediamine and oxidized dextran. The combination of magnetic nanoparticles and chemical cross-linked hydrogels leads to magnetic/pH dual-sensitive hydrogels which can be used as stimuli-responsive carrier. Magnetic properties, swelling, and rheology behaviors of the resulted magnetic hydrogels were strongly affected by the Fe3O4 nanoparticle content. Moreover, doxorubicin (DOX⋅HCl) was embedded into the magnetic hydrogels and pH/magnetic sensitive release profiles were identified. The release mechanism analysis indicated that the release behaviors of DOX⋅HCl were controlled by the diffusion, swelling, and erosion processes simultaneously. The prepared hydrogel/Fe3O4 composites with dual magnetic/pH stimuli-responsiveness hold the promise to be used in various applications such as drug release.

Multidimensional Profiles of Addictive Smartphone Use: A Latent Profile Analysis

Given that crucial psychological attributes of smartphone addiction have been studied in isolation from each other, we examined latent profiles of emotional distress (depression, stress, loneliness, and fear of missing out; i.e., FoMO); protective traits (self-control, mindfulness, grit); the behavioral inhibition system (BIS) and approach system (BAS; drive, reward responsiveness, and fun seeking) in relation to addictive smartphone use. We identified three distinctive profiles, using five fit statistics: AIC, BIC, adjusted BIC, an entropy, and LRT. The self-controlled, gritty, and mindful profile (22.7%) was characterized by heightened levels of self-control, grit, and mindfulness but lower levels of emotional distress, BIS, and BAS. The emotionally distressed profile (29.8%) was distinguished by elevated levels of depression, stress, loneliness, FoMO, and BIS, but relatively lower protective traits and BAS. Lastly, the approach sensitive profile (47.5%) corresponded to the normative group characterized by relatively higher BAS but mostly average levels of emotional distress and protective traits. When both global and pairwise comparisons between profiles were performed using Wald tests, we found that the self-controlled, gritty, and mindful profile was associated with significantly lower smartphone addiction tendencies than emotionally distressed or approach sensitive profiles, while the latter two did not differ from each other. These results still held when multiple covariates (age, sex, and income) were controlled for. Using a sophisticated person-centered approach, our findings underscore multidimensional psychological profiles that have different associations with smartphone addiction.